A temperature-controlled incubation shaker for reagent reactions

Abstract

The invention relates to the field of biomedical research, and in particular to a temperature-controlled incubation shaker for reagent reactions, comprising a control box and an elliptical incubation turntable, wherein a rotating motor is arranged in the control box, wherein the output shaft of the rotating motor is fixedly connected to the bottom of the elliptical incubation turntable, wherein a semiconductor refrigeration plate for adjusting the incubation temperature and a temperature sensor for measuring the real-time temperature are arranged in the elliptical incubation turntable, wherein a plurality of centrifugal test tubes for incubation are arranged in the elliptical incubation turntable, wherein a speed knob for controlling the speed of the elliptical incubation turntable, a touch screen for human-computer interaction and a switch button for turning on and off power are arranged on the control box, wherein the temperature-controlled incubation shaker for reagent reactions of the invention can provide the temperature conditions required for incubation while performing rocking incubation on biological reagents, and wherein the elliptical structure causes the centrifugal test tubes at different positions to be subjected to different centrifugal forces, thereby facilitating the study of the influence of the incubation conditions on the biological reagents.

Description

Technical Field

[0001] The invention relates to the field of biomedical research, and in particular to a temperature-controlled incubation shaker for reagent reactions. Background Art

[0002] The biological shakers in the laboratory now have a single function and can only shake at room temperature. However, some biomedical experimental reagents often need to be shaken and incubated at a certain temperature. For example, Western blot experiments require shaking and incubation at 4°C. At this time, the conventional laboratory shakers cannot meet the conditions required for the experiment. Therefore, we urgently need a biological shaker that can control the temperature. Summary of the invention

[0003] The object of the present invention is to provide a temperature-controlled incubation shaker for reagent reactions in view of the deficiencies of the prior art.

[0004] In order to solve the above problems, the present invention provides the following technical solutions:

[0005] A temperature-controlled incubation shaker for reagent reactions comprises a control box and an elliptical incubation turntable, wherein a rotating motor is arranged in the control box, an output shaft of the rotating motor vertically penetrates the control box upward and is fixedly connected to the bottom of the elliptical incubation turntable directly above the control box, a semiconductor refrigeration sheet for adjusting the incubation temperature and a temperature sensor for measuring the real-time temperature are arranged in the elliptical incubation turntable, a plurality of centrifugal test tubes for incubation are arranged in the elliptical incubation turntable, a speed knob for controlling the speed of the elliptical incubation turntable, a touch screen for human-computer interaction and a switch button for turning the power on and off are arranged on the control box.

[0006] Preferably, a shaft sleeve which is sleeved on the output shaft of the rotating motor is provided at the bottom of the oval incubation turntable, and the shaft sleeve and the output shaft of the rotating motor are locked by a latch which runs through the two.

[0007] Preferably, the elliptical incubation turntable includes a base plate and a cover plate, both of which are elliptical structures, and the top edge of the base plate and the bottom edge of the cover plate are snap-fitted with each other.

[0008] Preferably, a lower sponge for placing the centrifuge test tube is provided in the bottom plate, and an upper sponge for cooperating with the lower sponge to press the centrifuge test tube is provided at the bottom of the cover plate.

[0009] Preferably, the top of the lower sponge and the bottom of the upper sponge are both provided with tube grooves for accommodating centrifugal test tubes.

[0010] Preferably, the semiconductor refrigeration sheet is arranged at the bottom of the inner side of the chassis, the cold end of the semiconductor refrigeration sheet is in contact with the bottom of the lower sponge, and the hot end of the semiconductor refrigeration sheet is in contact with the inner bottom of the chassis.

[0011] Preferably, a heat dissipation grille for dissipating heat from the hot end of the semiconductor refrigeration plate is provided at the bottom of the chassis corresponding to the position of the semiconductor refrigeration plate.

[0012] Beneficial effects: The temperature-controlled incubation shaker for reagent reactions of the present invention, when in use, places the centrifugal test tube between the upper sponge and the lower sponge, the hollowed-out tube groove in the lower sponge can be used to place centrifugal test tubes of different specifications, and in the process of rotating incubation, the compression of the upper sponge is coordinated to ensure that the centrifugal test tubes in the elliptical incubation turntable will not collide with each other and break, after the centrifugal test tubes filled with reagents are placed in the elliptical incubation turntable, the cover plate is placed on the bottom plate to prevent the centrifugal test tube from falling off during rotation, and after the experimenter inputs the temperature data required for incubation through the touch screen, the control box controls the semiconductor refrigeration chip to start cooling, and the low temperature generated by the semiconductor refrigeration chip is transmitted to the upper centrifugal test tube through the lower sponge, thereby cooling the living organisms in the centrifugal test tube The biological reagents are provided with the required incubation temperature, and the temperature sensor arranged on the lower sponge displays the real-time temperature in the elliptical incubation turntable on the touch screen for the experimenter to know. The air flow during rotation enhances the heat dissipation efficiency of the hot end of the semiconductor refrigeration plate, and the heat of the hot end is taken away from the heat dissipation grille, ensuring that the cold end of the semiconductor refrigeration plate can be stably and effectively cooled during the rotating incubation process, thereby being able to stably provide the required temperature conditions for the biological reagents. The temperature-controlled incubation shaker for reagent reactions of the present invention can provide the required temperature conditions for incubation while shaking and incubating the biological reagents, and the elliptical structure causes the centrifugal test tubes in different positions to be subjected to different centrifugal forces, which is convenient for studying the influence of incubation conditions on biological reagents. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 Shown is a schematic diagram of the three-dimensional structure of the present invention;

[0014] Figure 2 Shown is a plan schematic diagram of the present invention;

[0015] Figure 3 Shown is the three-dimensional structure decomposition of the present invention Figure 1 ;

[0016] Figure 4 Shown is the three-dimensional structure decomposition of the present invention Figure 2 ;

[0017] Explanation of the reference numerals: control box 1, oval incubation turntable 2, semiconductor refrigeration sheet 3, temperature sensor 4, centrifuge tube 5, speed knob 6, touch screen 7, switch button 8, bushing 9, latch 11, chassis 12, cover plate 13, upper sponge 14, lower sponge 15, pipe groove 16, heat dissipation grille 17. DETAILED DESCRIPTION

[0018] The specific embodiments of the present invention are further described in detail below in conjunction with the accompanying drawings and embodiments of the specification:

[0019] Reference Figures 1 to 4 A temperature-controlled incubation shaker for reagent reactions shown in the figure comprises a control box 1 and an elliptical incubation turntable 2, wherein a rotating motor is arranged in the control box 1, and an output shaft of the rotating motor vertically penetrates the control box 1 upward and is fixedly connected to the bottom of the elliptical incubation turntable 2 directly above the control box 1, a semiconductor refrigeration plate 3 for adjusting the incubation temperature and a temperature sensor 4 for measuring the real-time temperature are arranged in the elliptical incubation turntable 2, a plurality of centrifugal test tubes 5 for incubation are arranged in the elliptical incubation turntable 2, and a speed knob 6 for controlling the speed of the elliptical incubation turntable 2, a touch screen 7 for human-computer interaction and a switch button 8 for turning the power on and off are arranged on the control box 1.

[0020] The bottom of the elliptical incubation turntable 2 is provided with a shaft sleeve 9 sleeved on the output shaft of the rotating motor. The shaft sleeve 9 and the output shaft of the rotating motor are locked by a latch 11 running through the two. The bottom of the elliptical incubation turntable 2 is synchronously locked with the rotating motor through the latch 11. After pressing the switch button 8, the control box 1 controls the rotating motor to start rotating. The rotation of the rotating motor drives the elliptical incubation turntable 2 to rotate, and then the centrifugal test tube 5 in the elliptical incubation turntable 2 rotates, thereby swinging and incubating the biological reagent in the centrifugal test tube 5. The elliptical structure makes the centrifugal force on the centrifugal test tube 5 at different positions different, so that the amplitude of swing incubation of the biological reagent is more diversified.

[0021] The elliptical incubation turntable 2 includes a base plate 12 and a cover plate 13, both of which are elliptical structures. The top edge of the base plate 12 and the bottom edge of the cover plate 13 are engaged with each other, and the edges of the base plate 12 and the cover plate 13 are provided with mutually engaging grooves. After the centrifugal test tube 5 filled with reagents is placed into the elliptical incubation turntable 2, the cover plate 13 is covered on the base plate 12 to prevent the centrifugal test tube 5 from falling off during rotation.

[0022] The bottom plate 12 is provided with a lower sponge 15 for placing the centrifuge test tube 5, and the bottom of the cover plate 13 is provided with an upper sponge 14 for cooperating with the lower sponge 15 to press the centrifuge test tube 5. The centrifuge test tube 5 is placed between the upper sponge 14 and the lower sponge 15. When the elliptical incubation turntable 2 rotates, the centrifuge test tube 5 can be well buffered and protected.

[0023] The top of the lower sponge 15 and the bottom of the upper sponge 14 are both provided with tube grooves 16 for accommodating centrifugal test tubes 5. The hollowed-out tube grooves 16 in the lower sponge 15 can be used to place centrifugal test tubes 5 of different specifications. During the rotating incubation process, the upper sponge 14 is pressed tightly to ensure that the centrifugal test tubes 5 in the elliptical incubation turntable 2 will not collide with each other and break.

[0024] The semiconductor refrigeration sheet 3 is arranged at the bottom of the inner side of the chassis 12, the cold end of the semiconductor refrigeration sheet 3 is in contact with the bottom of the lower sponge 15, and the hot end of the semiconductor refrigeration sheet 3 is in contact with the inner bottom of the chassis 12. After the experimenter inputs the temperature data required for incubation through the touch screen 7, the control box 1 controls the semiconductor refrigeration sheet 3 to start cooling. The low temperature generated by the operation of the semiconductor refrigeration sheet 3 is transmitted to the upper centrifuge test tube 5 through the lower sponge 15, thereby providing the required incubation temperature for the biological reagent in the centrifuge test tube 5. At the same time, the temperature sensor 4 arranged on the lower sponge 15 displays the real-time temperature in the elliptical incubation turntable 2 on the touch screen 7 for the experimenter to know.

[0025] A heat dissipation grille 17 for dissipating heat from the hot end of the semiconductor refrigeration plate 3 is provided at the bottom of the chassis 12 corresponding to the position of the semiconductor refrigeration plate 3. When the cold end of the semiconductor refrigeration plate 3 is cooled, the hot end will generate heat. The heat dissipation efficiency of the hot end of the semiconductor refrigeration plate 3 is enhanced by the flow of air during rotation, and the heat at the hot end is taken away from the heat dissipation grille 17, ensuring that the cold end of the semiconductor refrigeration plate 3 can be cooled stably and effectively during the rotary incubation process, thereby being able to stably provide the temperature conditions required by the biological reagent.

[0026] Working principle: When in use, the centrifugal test tube 5 is placed between the upper sponge 14 and the lower sponge 15. The hollowed-out tube groove 16 in the lower sponge 15 can be used to place centrifugal test tubes 5 of different specifications. During the rotational incubation process, the upper sponge 14 is pressed tightly to ensure that the centrifugal test tubes 5 in the elliptical incubation turntable 2 will not collide with each other and break. After the centrifugal test tube 5 filled with reagents is placed in the elliptical incubation turntable 2, the cover plate 13 is covered on the bottom plate 12 to prevent the centrifugal test tube 5 from falling off during rotation. After the experimenter inputs the temperature data required for incubation through the touch screen 7, the control box 1 controls the semiconductor refrigeration chip 3 to start cooling. The low temperature generated by the semiconductor refrigeration chip 3 is transmitted to the upper centrifugal test tube 5 through the lower sponge 15, thereby providing the required incubation temperature for the biological reagent in the centrifugal test tube 5. At the same time, the lower sponge 15 is set The temperature sensor 4 on the elliptical incubation turntable 2 displays the real-time temperature on the touch screen 7 for the experimenter to know. The air flow during rotation enhances the heat dissipation efficiency of the hot end of the semiconductor refrigeration plate 3. The heat of the hot end is taken away from the heat dissipation grille 17, ensuring that the cold end of the semiconductor refrigeration plate 3 can be stably and effectively cooled during the rotating incubation process, thereby being able to stably provide the temperature conditions required by the biological reagent.

[0027] The above description is only a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any slight modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A temperature-controlled incubation shaker for reagent reactions, characterized in that: The invention comprises a control box (1) and an elliptical incubation turntable (2), wherein a rotating motor is arranged in the control box (1), an output shaft of the rotating motor vertically passes through the control box (1) upward and is fixedly connected to the bottom of the elliptical incubation turntable (2) directly above the control box (1), a semiconductor cooling plate (3) for adjusting the incubation temperature and a temperature sensor (4) for measuring the real-time temperature are arranged in the elliptical incubation turntable (2), a plurality of centrifugal test tubes (5) for incubation are arranged in the elliptical incubation turntable (2), a speed knob (6) for controlling the speed of the elliptical incubation turntable (2), a touch screen (7) for human-computer interaction and a switch button (8) for turning on and off the power are arranged on the control box (1); a shaft sleeve (9) sleeved on the output shaft of the rotating motor is arranged at the bottom of the elliptical incubation turntable (2), and the shaft sleeve (9) and the output shaft of the rotating motor are locked by a latch (11) passing through the two; the elliptical incubation turntable (2) comprises a chassis (12 ) and a cover plate (13), the bottom plate (12) and the cover plate (13) are both elliptical structures, the top edge of the bottom plate (12) and the bottom edge of the cover plate (13) are mutually engaged; a lower sponge (15) for accommodating the centrifugal test tube (5) is arranged in the bottom plate (12), and an upper sponge (14) for cooperating with the lower sponge (15) to press the centrifugal test tube (5) is arranged at the bottom of the cover plate (13); the top of the lower sponge (15) and the upper sponge (14) are connected to each other. The bottom of each of the semiconductor refrigeration plates (12) is provided with a tube groove (16) for accommodating a centrifugal test tube (5); the semiconductor refrigeration plate (3) is arranged at the bottom of the inner side of the chassis (12); the cold end of the semiconductor refrigeration plate (3) is in contact with the bottom of the lower sponge (15); and the hot end of the semiconductor refrigeration plate (3) is in contact with the inner bottom of the chassis (12); a heat dissipation grille (17) for dissipating heat from the hot end of the semiconductor refrigeration plate (3) is arranged at a position on the bottom of the chassis (12) corresponding to the semiconductor refrigeration plate (3);When in use, the centrifugal test tube (5) is placed between the upper sponge (14) and the lower sponge (15). The hollowed-out tube groove (16) in the lower sponge (15) can be used to place centrifugal test tubes (5) of different specifications. During the rotational incubation process, the upper sponge (14) is pressed tightly to ensure that the centrifugal test tubes (5) in the elliptical incubation turntable (2) will not collide with each other and break. After the centrifugal test tube (5) containing the reagent is placed in the elliptical incubation turntable (2), the cover plate (13) is placed on the bottom plate (12) to prevent the centrifugal test tube (5) from falling off during rotation. After the experimenter inputs the temperature data required for incubation through the touch screen (7), the control box (1) controls the semiconductor cooling plate (3) to work. The temperature starts to drop, and the low temperature generated by the semiconductor refrigeration sheet (3) is transmitted to the upper centrifugal test tube (5) through the lower sponge (15), thereby providing the required incubation temperature for the biological reagent in the centrifugal test tube (5). At the same time, the temperature sensor (4) arranged on the lower sponge (15) displays the real-time temperature in the elliptical incubation turntable (2) on the touch screen (7) for the experimenter to know. The airflow during rotation enhances the heat dissipation efficiency of the hot end of the semiconductor refrigeration sheet (3), and the heat of the hot end is taken away from the heat dissipation grille (17), ensuring that the cold end of the semiconductor refrigeration sheet (3) can stably and effectively drop the temperature during the rotation incubation process, thereby stably providing the required temperature conditions for the biological reagent. ;

Images