Liquid chromatographic fraction collector and use method

Abstract

The invention provides a liquid chromatographic fraction collector and a use method. The fraction collector and a spray drying system are organically combined, components prepared and enriched throughliquid chromatography are atomized by a spray drying nozzle and then dried under negative pressure or at high temperature in a drying room to form solids, the solids are caught by a receiving pipe, solid products of the components are collected, labor and time cost are saved on the basis of high separation efficiency and low labor intensity, and the collector has the characteristics of being compact in structure, small in size, easy to operate and automatize, convenient to move and clean and good in drying effect, and solids of liquid chromatographic separation components can be rapidly, effectively and intelligently collected.

Description

technical field

[0001] The invention belongs to the technical field of chromatographic instruments and equipment, and in particular relates to a liquid chromatographic fraction collector and a usage method. Background technique

[0002] With the development of analytical instruments, liquid chromatography is widely used in the fields of life science, traditional Chinese medicine, biochemical industry, medicine and scientific research as an effective method for sample recovery or sample purification. However, the sample prepared by liquid chromatography is a liquid. In order to obtain a solid state sample, a series of post-treatments are required, including rotary evaporation, spray drying, enrichment, centrifugation, freeze-drying and other steps. These offline operations need to be processed step by step, which has problems such as time-consuming, cumbersome steps, and easy introduction of pollutants. Two-dimensional liquid chromatography has received widespread attention ...

Examples

Embodiment 1

[0032] see figure 1 , figure 1 It is a schematic diagram of the work flow of a liquid chromatography fraction collector of the present invention. Such as figure 1 As shown, the liquid chromatography fraction collector includes two parts: a spray drying device (not shown) and a vacuum trapping device (not shown).

[0033] The above two parts are described below:

[0034] read on figure 1 , figure 1 It is a schematic diagram of the work flow of a liquid chromatography fraction collector of the present invention. Such as figure 1 As shown, the spray drying device includes an electric heater (not shown), a spray drying nozzle 3, an auxiliary gas interface (not shown), a spray drying chamber 4 and a movable spray drying bracket 5, and the spray drying nozzle 3 is connected to the liquid phase The chromatographic system 1 is connected through a liquid pipeline 2, the auxiliary gas interface is connected to the spray drying nozzle 3, and the auxiliary gas interface is connecte...

Embodiment 2

[0037] The present invention also introduces another square material-receiving pipe seat structure, which is the same as that of Embodiment 1, with only two differences. For specific differences, please refer to Figure 4 and Figure 5 , Figure 4 It is a structural schematic diagram of a receiving tube seat of a liquid chromatography fraction collector of the present invention in embodiment 2; Figure 5 It is a schematic top view of the structure of a liquid chromatography fraction collector of the present invention in Example 2. Such as Figure 4 and Figure 5 As shown, the spray drying support 5 can move in the X and Y axis directions, and the material receiving tube base 7 is square.

technical field

[0001] The invention belongs to the technical field of chromatographic instruments and equipment, and in particular relates to a liquid chromatographic fraction collector and a usage method. Background technique

[0002] With the development of analytical instruments, liquid chromatography is widely used in the fields of life science, traditional Chinese medicine, biochemical industry, medicine and scientific research as an effective method for sample recovery or sample purification. However, the sample prepared by liquid chromatography is a liquid. In order to obtain a solid state sample, a series of post-treatments are required, including rotary evaporation, spray drying, enrichment, centrifugation, freeze-drying and other steps. These offline operations need to be processed step by step, which has problems such as time-consuming, cumbersome steps, and easy introduction of pollutants. Two-dimensional liquid chromatography has received widespread attention ...

Examples

Embodiment 1

[0032] see figure 1 , figure 1 It is a schematic diagram of the work flow of a liquid chromatography fraction collector of the present invention. Such as figure 1 As shown, the liquid chromatography fraction collector includes two parts: a spray drying device (not shown) and a vacuum trapping device (not shown).

[0033] The above two parts are described below:

[0034] read on figure 1 , figure 1 It is a schematic diagram of the work flow of a liquid chromatography fraction collector of the present invention. Such as figure 1 As shown, the spray drying device includes an electric heater (not shown), a spray drying nozzle 3, an auxiliary gas interface (not shown), a spray drying chamber 4 and a movable spray drying bracket 5, and the spray drying nozzle 3 is connected to the liquid phase The chromatographic system 1 is connected through a liquid pipeline 2, the auxiliary gas interface is connected to the spray drying nozzle 3, and the auxiliary gas interface is connecte...

Embodiment 2

[0037] The present invention also introduces another square material-receiving pipe seat structure, which is the same as that of Embodiment 1, with only two differences. For specific differences, please refer to Figure 4 and Figure 5 , Figure 4 It is a structural schematic diagram of a receiving tube seat of a liquid chromatography fraction collector of the present invention in embodiment 2; Figure 5 It is a schematic top view of the structure of a liquid chromatography fraction collector of the present invention in Example 2. Such as Figure 4 and Figure 5 As shown, the spray drying support 5 can move in the X and Y axis directions, and the material receiving tube base 7 is square.

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