A high-concentration, long-term cell preservation and transportation device at room temperature

Abstract

The present invention relates to a high-concentration, long-term cell preservation and transportation device at room temperature, comprising a first outer shell, a first inner shell arranged in the inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell arranged on the side wall of the first inner shell, and a permeation membrane connected to the first inner shell arranged at the permeation hole. The high-concentration, long-term cell preservation and transportation device at room temperature of the present invention is suitable for long-distance, long-term transportation of high-concentration cell fluid through the arrangement of the first outer shell and the first inner shell, and the cell fluid is not easily contaminated.

Description

Technical Field

[0001] The invention relates to a cell transport device, in particular to a preservation and transportation device which can transport and preserve high-concentration cells for a long time at room temperature. Background Art

[0002] At present, the method used to store and transport fresh cells at room temperature is to seal the cells and culture medium in a culture dish. In order to ensure that the cells can be provided with sufficient nutritional support during transportation and to ensure that the cell metabolic products can be effectively diluted, the cells are usually distributed in the culture medium at a lower concentration, such as 1×10 5 / ml. After arriving at the destination, the transported cells need to be centrifuged and concentrated to a suitable concentration, or they need to be cultured for several days. The former increases the process and the cells are easily contaminated, while the latter treatment method prevents laboratory and clinical operators from using the cells immediately. Both require the receiving unit to have certain laboratory equipment. Therefore, it is not conducive to the direct clinical use of cell therapy products. On the other hand, in order to prevent the vibration of the culture medium during transportation from causing the cells to collide with the wall of the culture dish and cause cell death, and to avoid the formation of air bubbles in the culture medium and cause protein inactivation, the culture dish is generally filled with culture medium to remove residual air. However, this approach cannot guarantee that the cells continue to get air during long-term transportation, especially the high concentration of carbon dioxide therein. In addition, since the culture medium is in direct contact with the mouth of the culture dish, if there is any carelessness in the operation, external bacteria may enter the test tube through a trace amount of culture medium in the sealing plug, thereby contaminating the cells in the test tube.

[0003] Due to the above reasons, after long-distance transportation, the survival rate of cells is often low (usually around 50% to 60%), which cannot meet the requirements of use. If the transportation is long-distance and long-term, the cells may even be unable to recover due to the low survival rate.

[0004] Obviously, the current solutions to several key issues in cell transportation methods cannot take into account several issues such as cell concentration, cell culture medium, and carbon dioxide-rich air (for example, as mentioned earlier, in order to ensure the survival rate of cells during transportation, sufficient culture medium needs to be stored in the test tube to provide nutrients for the cells. However, the addition of culture medium reduces the concentration of cells. After arriving at the transportation destination, operators also need to concentrate the cell fluid before use, which not only increases the workload, but also increases the possibility of external contamination). These limitations seriously affect the long-distance and long-term transportation of fresh cells at room temperature. Therefore, in the case of large-scale clinical cell therapy, the current cell transportation method urgently needs to be revolutionary.

[0005] In view of the above-mentioned defects, the designers have actively carried out research and innovation in order to create a cell preservation device with a new structure to make it more valuable for industrial use. Summary of the invention

[0006] In order to solve the above technical problems, the purpose of the present invention is to provide a high-concentration long-term cell preservation and transportation device at room temperature that is suitable for long-distance and long-term transportation of high-concentration cell fluid and is not easily contaminated.

[0007] The high-concentration, long-term cell preservation and transportation device at room temperature of the present invention comprises a first outer shell, a first inner shell is arranged in the inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell is arranged on the side wall of the first inner shell, and a permeation membrane connected to the first inner shell is arranged at the permeation hole.

[0008] Through the above scheme, the present invention has at least the following advantages: the high-concentration long-term cell preservation and transportation device at room temperature of the present invention comprises a first outer shell and a first inner shell, and a permeable membrane is arranged on the first inner shell. When in use, the operator places the cell fluid into the first inner shell and the culture fluid into the first outer shell. The setting of the permeable membrane enables the culture fluid in the inner cavity of the first outer shell to enter the first inner shell through the permeable membrane, thereby providing nutrients for the cells in the first inner shell. In this way, the operator can store a high-concentration cell fluid in the first inner shell. At the same time, the setting of the external culture fluid ensures that the high-concentration cell fluid will not reduce its survival rate due to long-term transportation. After being transported to the predetermined target, the cell fluid can be used directly without concentration. In addition, since the cell fluid is stored in the first inner shell, it is not directly arranged in the culture fluid, and the cells and the culture fluid are arranged separately, thereby reducing the risk of contamination.

[0009] In summary, the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention is suitable for long-distance, long-term transportation of high-concentration cell fluid, and the cell fluid is not easily contaminated.

[0010] Furthermore, in the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention, the top end of the first outer shell is provided with a top cover connected to the first outer shell, and the top end of the first inner shell is provided on the top cover.

[0011] Furthermore, in the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention, the first outer shell and the first inner shell are both connected to the top cover via threads.

[0012] Furthermore, in the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention, the top cover includes an upper cover body, a lower cover body located below the upper cover body, and a first air-permeable membrane located between the upper cover body and the lower cover body, and the upper cover body and the lower cover body are provided with ventilation holes connected to the inner cavity of the first outer shell or the inner cavity of the first inner shell. (The ventilation holes are for air or carbon dioxide to enter the cell fluid or culture fluid, otherwise the carbon dioxide and air set in the upper shell cannot enter the lower shell, and the upper shell loses its function)

[0013] The provision of the vent holes allows external air to enter the first shell through the vent holes and the breathable membrane, thereby providing air for the culture solution in the first shell, thereby increasing the air content in the culture solution and further increasing the survival rate of the cells.

[0014] Furthermore, in the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention, a second shell is provided above the first shell.

[0015] Furthermore, in the high-concentration, long-time cell preservation and transportation device at room temperature of the present invention, a second inner shell is further provided in the second outer shell, the second outer shell and the second inner shell are both connected to the top cover, the side wall of the second inner shell is provided with an air hole penetrating the side wall of the second inner shell, a second air permeable membrane connected to the second inner shell is provided at the air hole, the air hole is located in the middle of the top cover, and the air hole is respectively connected to the inner cavity of the second inner shell and the inner cavity of the first inner shell.

[0016] During long-distance transportation, multiple high-concentration, long-term cell preservation and transportation devices at room temperature are simultaneously arranged in a relatively closed space, such as a storage tank in a box body, so the air content is relatively low. For this reason, the second outer shell is arranged so that the operator can store a certain amount of air in the second outer shell in advance, thereby increasing the survival rate of the cells and providing the possibility for long-term storage and transportation of the cell fluid. The second inner shell is arranged so that the gas in the first inner shell can enter the first inner shell through the second breathable membrane, the vent and the first breathable membrane on the second inner shell, thereby further isolating the external air from the inner cavity of the first inner shell, thereby preventing the cell fluid from being contaminated.

[0017] Furthermore, in the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention, rubber stoppers are provided on the side walls of the first shell and the second shell.

[0018] The provision of the rubber stopper enables the operator to inject culture fluid into the first shell or inject gas into the second shell through the rubber stopper, and the culture fluid or gas is not easily contaminated.

[0019] Furthermore, in the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention, a mixed gas is arranged in the second shell, and the mixed gas includes air and carbon dioxide, and the volume ratio of the carbon dioxide to air is 5% to 10%.

[0020] The carbon dioxide and air ratio of 5% is most suitable for cell growth. Carbon dioxide can be formed by baking soda arranged in the second shell.

[0021] Furthermore, in the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention, the volume ratio of the first inner shell to the first outer shell is 1:10 to 1:50, and the volume ratio of the first inner shell to the second outer shell is 1:10 to 1:50.

[0022] Furthermore, in the high-concentration, long-term cell preservation and transportation device at room temperature of the present invention, cell fluid is provided in the first inner shell, and culture fluid is provided in the first outer shell.

[0023] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the structure of the high-concentration, long-term cell preservation and transportation device at room temperature according to Example 1;

[0025] Figure 2 This is a schematic diagram of the structure of a high-concentration, long-term cell preservation and transportation device at room temperature according to Example 2;

[0026] Figure 3 This is a schematic diagram of the structure of a high-concentration, long-term cell preservation and transportation device at room temperature according to Example 2;

[0027] Figure 4 1 is a schematic diagram of the structure of a high-concentration, long-term cell preservation and transportation device at room temperature according to Embodiment 4;

[0028] Figure 5 1 is a schematic diagram of the structure of a high-concentration, long-term cell preservation and transportation device at room temperature according to Embodiment 5;

[0029] Figure 6 is a schematic diagram of the structure of a high-concentration, long-term cell preservation and transportation device at room temperature according to Example 6;

[0030] Figure 7 It is a cell viability curve graph corresponding to Table 1 of Example 1.

[0031] In the figure, 1: first outer shell; 2: first inner shell; 3: permeable membrane; 4: top cover; 5: upper cover body; 6: lower cover body; 7: first breathable membrane; 8: vent hole; 9: rubber plug; 10: cell fluid; 11: culture medium; 12: second outer shell; 13: second inner shell; 14: second breathable membrane. DETAILED DESCRIPTION

[0032] The specific implementation of the present invention is further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0033] Embodiment 1:

[0034] See also Figure 1 , 7 The high-concentration, long-term cell preservation and transportation device at room temperature of this embodiment includes a first outer shell 1, a first inner shell 2 is arranged in the inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell is arranged on the side wall of the first inner shell, and a permeation membrane 3 connected to the first inner shell is arranged at the permeation hole.

[0035] Preferably, a top cover 4 connected to the first outer shell is provided at the top end of the first outer shell, and a top end of the first inner shell is provided on the top cover.

[0036] Preferably, the first outer shell and the first inner shell are connected to the top cover via threads.

[0037] Preferably, the top cover includes an upper cover body 5, a lower cover body 6 located below the upper cover body, and a first breathable membrane 7 located between the upper cover body and the lower cover body. The upper cover body and the lower cover body are correspondingly provided with ventilation holes 8 connected to the inner cavity of the first shell.

[0038] Preferably, a rubber stopper 9 is provided on the side wall of the first shell.

[0039] Preferably, cell fluid 10 is provided in the first inner shell, and culture fluid 11 is provided in the first outer shell.

[0040] During specific use, the operator places the cell fluid into the first inner shell and the culture fluid into the first outer shell. The setting of the osmotic membrane allows the culture fluid in the inner cavity of the first outer shell to enter the first inner shell through the osmotic membrane, thereby providing nutrients for the cells in the first inner shell. In this way, the operator can store a high-concentration cell fluid in the first inner shell. At the same time, the setting of the culture fluid ensures that the high-concentration cell fluid will not reduce its survival rate due to long-term transportation. After being transported to the predetermined target, the cell fluid can be used directly without concentration. In addition, since the cell fluid is stored in the first inner shell, it is not directly set in the culture fluid, and the cells and the culture fluid are set separately, thereby reducing the risk of contamination.

[0041] The following table 1 is the initial concentration of 4×107 The results showed that after one week of storage, the high-concentration cells preserved by the traditional method had been reduced to half, while the cells preserved by the present method still had a survival rate of more than 80%.

[0042]

[0043] Table 1: 4X10 7 Comparison of cell / ml survival rate at 25℃

[0044] Embodiment 2:

[0045] See also Figure 2 The high-concentration, long-term cell preservation and transportation device at room temperature of this embodiment includes a first outer shell 1, a first inner shell 2 is arranged in the inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell is arranged on the side wall of the first inner shell, and a permeation membrane 3 connected to the first inner shell is arranged at the permeation hole.

[0046] Preferably, a top cover 4 connected to the first outer shell is provided at the top end of the first outer shell, and a top end of the first inner shell is provided on the top cover.

[0047] Preferably, the first outer shell and the first inner shell are connected to the top cover via threads.

[0048] Preferably, the top cover includes an upper cover body 5, a lower cover body 6 located below the upper cover body, and a first breathable membrane 7 located between the upper cover body and the lower cover body. The upper cover body and the lower cover body are correspondingly provided with ventilation holes 8 connected to the inner cavity of the first inner shell.

[0049] Preferably, a rubber stopper 9 is provided on the side wall of the first shell.

[0050] Preferably, cell fluid 10 is provided in the first inner shell, and culture fluid 11 is provided in the first outer shell.

[0051] In this embodiment, the vent hole is located in the middle of the top cover, which connects the atmosphere with the inner cavity of the first inner shell, so that the cell fluid can directly absorb air from the atmosphere.

[0052] Embodiment three:

[0053] See also Figure 3 The high-concentration, long-term cell preservation and transportation device at room temperature of this embodiment includes a first outer shell 1, a first inner shell 2 is arranged in the inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell is arranged on the side wall of the first inner shell, and a permeation membrane 3 connected to the first inner shell is arranged at the permeation hole.

[0054] Preferably, a top cover 4 connected to the first outer shell is provided at the top end of the first outer shell, and a top end of the first inner shell is provided on the top cover.

[0055] Preferably, the first outer shell and the first inner shell are connected to the top cover via threads.

[0056] Preferably, the top cover includes an upper cover body 5, a lower cover body 6 located below the upper cover body, and a first breathable membrane 7 located between the upper cover body and the lower cover body. The upper cover body and the lower cover body are correspondingly provided with ventilation holes 8 connected to the inner cavity of the first shell.

[0057] A second housing 12 is disposed above the first housing.

[0058] Preferably, rubber plugs are provided on the side walls of the first shell and the second shell.

[0059] Preferably, cell fluid is provided in the first inner shell, and culture fluid is provided in the first outer shell.

[0060] This embodiment adds a second shell. During long-distance transportation, multiple high-concentration, long-term cell preservation and transportation devices at room temperature are simultaneously arranged in a relatively closed space, such as a storage tank in a box body, so the air content is relatively small. Therefore, the second shell is arranged so that the operator can store a certain amount of air in the second shell in advance, thereby increasing the survival rate of the cells and providing the possibility for long-term storage and transportation of cell fluid.

[0061] Embodiment 4:

[0062] See also Figure 4 The high-concentration, long-term cell preservation and transportation device at room temperature of this embodiment includes a first outer shell 1, a first inner shell 2 is arranged in the inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell is arranged on the side wall of the first inner shell, and a permeation membrane 3 connected to the first inner shell is arranged at the permeation hole.

[0063] Preferably, a top cover 4 connected to the first outer shell is provided at the top end of the first outer shell, and a top end of the first inner shell is provided on the top cover.

[0064] Preferably, the first outer shell and the first inner shell are connected to the top cover via threads.

[0065] Preferably, the top cover includes an upper cover body 5, a lower cover body 6 located below the upper cover body, and a first breathable membrane 7 located between the upper cover body and the lower cover body. The upper cover body and the lower cover body are correspondingly provided with ventilation holes 8 connected to the inner cavity of the first inner shell.

[0066] A second housing 9 is provided above the first housing.

[0067] Preferably, rubber plugs are provided on the side walls of the first shell and the second shell.

[0068] Preferably, cell fluid is provided in the first inner shell, and culture fluid is provided in the first outer shell.

[0069] Compared with the third embodiment, the vent hole in this embodiment is located in the middle of the top cover, which connects the inner cavity of the first outer shell and the first inner shell, so that the cell fluid can directly absorb air from the first outer shell.

[0070] Embodiment five:

[0071] See also Figure 5 The high-concentration, long-term cell preservation and transportation device at room temperature of this embodiment includes a first outer shell 1, a first inner shell 2 is arranged in the inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell is arranged on the side wall of the first inner shell, and a permeation membrane 3 connected to the first inner shell is arranged at the permeation hole.

[0072] Preferably, a top cover 4 connected to the first outer shell is provided at the top end of the first outer shell, and a top end of the first inner shell is provided on the top cover.

[0073] Preferably, the first outer shell and the first inner shell are connected to the top cover via threads.

[0074] Preferably, the top cover includes an upper cover body 5, a lower cover body 6 located below the upper cover body, and a first breathable membrane 7 located between the upper cover body and the lower cover body. The upper cover body and the lower cover body are correspondingly provided with ventilation holes 8 connected to the inner cavity of the first shell.

[0075] A second housing 9 is provided above the first housing.

[0076] A second inner shell 13 is also provided in the second outer shell. Both the second outer shell and the second inner shell are connected to the top cover. A ventilation hole penetrating the side wall of the second inner shell is provided on the side wall of the second inner shell. A second ventilation membrane 14 connected to the second inner shell is provided at the ventilation hole. The ventilation hole is located in the middle of the top cover, and the ventilation holes are respectively connected to the inner cavity of the second inner shell and the inner cavity of the first inner shell.

[0077] Preferably, rubber plugs are provided on the side walls of the first shell and the second shell.

[0078] Preferably, cell fluid is provided in the first inner shell, and culture fluid is provided in the first outer shell.

[0079] This embodiment adds a second inner shell, which makes the cell fluid less likely to be contaminated by the outside.

[0080] Preferably, a mixed gas is arranged in the second shell, the mixed gas includes air and carbon dioxide, and the volume ratio of carbon dioxide to air is 5% to 10%.

[0081] The carbon dioxide and air ratio of 5% is most suitable for cell growth. Carbon dioxide can be formed by baking soda arranged in the second shell.

[0082] Preferably, the volume ratio of the first inner shell to the first outer shell is 1:10 to 1:50, and the volume ratio of the first inner shell to the second outer shell is 1:10 to 1:50.

[0083] Embodiment six:

[0084] See also Figure 6 The high-concentration, long-term cell preservation and transportation device at room temperature of this embodiment includes a first outer shell 1, a first inner shell 2 is arranged in the inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell is arranged on the side wall of the first inner shell, and a permeation membrane 3 connected to the first inner shell is arranged at the permeation hole.

[0085] Preferably, a top cover 4 connected to the first outer shell is provided at the top end of the first outer shell, and a top end of the first inner shell is provided on the top cover.

[0086] Preferably, the first outer shell and the first inner shell are connected to the top cover via threads.

[0087] Preferably, the top cover includes an upper cover body 5, a lower cover body 6 located below the upper cover body, and a first breathable membrane 7 located between the upper cover body and the lower cover body. The upper cover body and the lower cover body are correspondingly provided with ventilation holes 8 connected to the inner cavity of the first shell.

[0088] A second inner shell 13 is also provided, the second inner shell is connected to the top cover, a ventilation hole penetrating the side wall of the second inner shell is provided on the side wall of the second inner shell, a second ventilation membrane 14 connected to the second inner shell is provided at the ventilation hole, the ventilation hole is located in the middle of the top cover, and the ventilation hole is respectively connected to the inner cavity of the second inner shell and the inner cavity of the first inner shell.

[0089] Preferably, a rubber stopper is provided on the side wall of the first shell.

[0090] Preferably, cell fluid is provided in the first inner shell, and culture fluid is provided in the first outer shell.

[0091] Compared with the fifth embodiment, the second housing is not provided in this embodiment.

[0092] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that those skilled in the art can implement the present invention in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive in all respects, and the scope of protection of the present invention is limited by the appended claims rather than the above description.

[0093] In addition, the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention. At the same time, it should be understood that although this specification is described in accordance with the implementation mode, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A high-concentration, long-term cell storage and transportation device at room temperature, characterized in that: It comprises a first outer shell, wherein a first inner shell is arranged in an inner cavity of the first outer shell, a permeation hole penetrating the side wall of the first inner shell is arranged on a side wall of the first inner shell, and a permeation membrane connected to the first inner shell is arranged at the permeation hole; The top end of the first outer shell is provided with a top cover connected to the first outer shell, and the top end of the first inner shell is provided on the top cover; The top cover comprises an upper cover body, a lower cover body located below the upper cover body, and a first breathable membrane located between the upper cover body and the lower cover body, and the upper cover body and the lower cover body are respectively provided with vent holes communicating with the inner cavity of the first outer shell or the inner cavity of the first inner shell; The first inner shell contains cell fluid, and the first outer shell contains culture fluid; A second housing is provided above the first housing; A second inner shell is further provided in the second outer shell, and the second outer shell and the second inner shell are both connected to the top cover. A vent hole penetrating the side wall of the second inner shell is provided on the side wall of the second inner shell, and a second vent film connected to the second inner shell is provided at the vent hole. The vent hole is located in the middle of the top cover, and the vent hole is communicated with the inner cavity of the second inner shell and the inner cavity of the first inner shell respectively. A mixed gas is arranged in the second shell, and the mixed gas includes air and carbon dioxide.

2. The high-concentration, long-term cell storage and transportation device at room temperature according to claim 1, characterized in that: The first outer shell and the first inner shell are both connected to the top cover via threads.

3. The high-concentration, long-term cell storage and transportation device at room temperature according to claim 1, characterized in that: Rubber plugs are disposed on the side walls of the first shell and the second shell.

4. The high-concentration, long-term cell storage and transportation device at room temperature according to claim 1, characterized in that: A mixed gas is arranged in the second shell, and the mixed gas includes air and carbon dioxide, and the volume ratio of the carbon dioxide to the air is 5% to 10%.

5. The high-concentration, long-term cell storage and transportation device at room temperature according to claim 1, characterized in that: The volume ratio of the first inner shell to the first outer shell is 1:10-1:50, and the volume ratio of the first inner shell to the second outer shell is 1:10-1:50.

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