A clamping type immunoblotting analyzer and its control system

Abstract

The present invention provides a clamping type immunoblot analyzer and a control system thereof. The clamping type immunoblot analyzer comprises: a base, a sample film clip, a sample transfer zone, a reaction temperature zone and a transfer unit. The transfer unit comprises a chuck and an XYZ three-axis moving mechanism. The chuck is provided with a mechanism for taking and placing the sample film clip, and the XYZ three-axis moving mechanism drives the chuck to move between the sample transfer zone and each reaction temperature zone. The present invention also provides a reagent storage area to facilitate the recycling of expensive reagents and reduce experimental costs. The immunoblot analyzer can insert a new sample film for reaction at any time, and the test steps and conditions of each sample film can be set separately, which is more suitable for the test operation of laboratory personnel, greatly improving the use efficiency of the immunoblot analyzer, reducing the waiting time in line, and improving the test speed.

Description

Technical Field

[0001] The invention relates to a biological test auxiliary equipment, in particular to a clamping type immunoblotting analyzer and a control system thereof. Background Art

[0002] Immunoblotting is also known as protein blotting (Western Blot). It is a commonly used experimental method in biochemistry and immunogenetics. It is to transfer the total protein of cells or tissues separated by electrophoresis from the gel to the solid phase carrier NC membrane or PVDF membrane, and then use the protein or polypeptide on the solid phase carrier as an antigen to react with the corresponding antibody, and then react with the enzyme or isotope-labeled second antibody, and finally detect the protein components expressed by the specific target gene separated by electrophoresis through substrate color development or radioautography. In the entire immune reaction test, the solid phase carrier membrane needs to be washed-incubated-washed with multiple test solutions and multiple frequencies, which is time-consuming, high-frequency operation, and high-intensity work for the experimenter.

[0003] The small-scale immunoblot analyzers currently used in laboratories generally place samples in reaction dishes. During the experiment, test solutions are added to the reaction dishes. After a single reaction is completed, the test solutions are sucked out with a pipette and cleaned. The discarded primary antibody test solution and secondary antibody test solution contain a large amount of unconsumed antibodies, resulting in a serious waste of primary and secondary antibodies. In addition, the prices of primary and secondary antibodies are relatively expensive, resulting in higher experimental costs.

[0004] In the patent document of publication number CN109187990A, a kind of immunoblot analyzer is disclosed, which is clamped by a sample film clamping mechanism, and then driven by a mobile mechanism to move between each test liquid box, and an immune reaction is performed. Compared with the method of sucking test liquid in a sample film reaction dish, it can greatly save the usage of test liquid. The above-mentioned immunoblot analyzer can only perform a single group of synchronous experiments, and the experiment of the next group of sample films can only be performed after the single group of programs are completely finished. In actual scientific research experiments, there are often multiple groups that need to use different programs to perform immunoblot experiments, and the experiment start time is also different. For a group of immunoblot experiments that take several hours for a single time, the utilization rate of the instrument is not high, and the laboratory needs to be equipped with multiple units at the same time, which seriously occupies the experimental space and also increases the purchase cost. In addition, for some expensive reusable primary and secondary antibody test solutions, low temperature storage is required immediately after the reaction is completed. In the existing immunoblot analyzer, it is generally realized by using temperature control area temperature change, and the temperature change process is relatively slow, time-consuming, and energy-consuming. Summary of the invention

[0005] In order to overcome the above-mentioned deficiencies of the prior art, the object of the present invention is to provide a clamp-type immunoblotting analyzer and a control system thereof.

[0006] In order to achieve the above-mentioned object, the technical solution adopted by the present invention to solve the technical problem is: a clamp-type immunoblot analyzer, comprising:

[0007] Base;

[0008] A sample membrane clamp, which is used to clamp a sample membrane containing a protein or polypeptide to be analyzed;

[0009] A sample placement transfer area, which is provided with a receiving block for placing sample film clips to be analyzed or analyzed;

[0010] Multiple reaction temperature zones, each of which provides temperature conditions that match the test steps, and each reaction temperature zone is equipped with a temperature bath block for placing the reagent kit;

[0011] The transfer unit includes a chuck and an XYZ three-axis moving mechanism. The chuck is provided with a mechanism for taking and placing the sample film clip. The fixed end of the XYZ three-axis moving mechanism is installed on the base. The chuck is installed on the end moving block of the XYZ three-axis moving mechanism. The XYZ three-axis moving mechanism drives the chuck to move between the sample placement transfer zone and each reaction temperature zone.

[0012] Compared with the prior art, the present invention places the reagent kit required for the test in the reaction temperature zone, clamps the sample membrane to be analyzed by the sample membrane clamp, and places the sample membrane clamp in the sample placement transfer zone, and the transfer unit automatically clamps the sample membrane clamp from the sample placement transfer zone to the reagent kit in each reaction temperature zone for reaction; when a new sample membrane needs to be tested and analyzed, it only needs to be clamped on another sample membrane clamp and then directly placed in the sample placement transfer zone, and the transfer unit will automatically move the new sample membrane clamp into the reaction temperature zone for reaction according to the test steps; the immunoblot analyzer can insert new sample membranes for reaction at any time, and the test steps and conditions of each sample membrane can be set separately, which is more suitable for the test operation of laboratory personnel, greatly improving the use efficiency of the immunoblot analyzer, reducing queuing waiting time, and increasing the test speed.

[0013] Furthermore, it also includes a washing area, which includes a washing box, a washing liquid adding mechanism and a washing liquid discarding mechanism, the washing liquid adding mechanism is used to add washing liquid into the washing box, and the washing liquid discarding mechanism is used to discharge the waste liquid in the washing box; the transfer unit can drive the sample membrane clip to move to the washing area and place the sample membrane clip into the washing box.

[0014] By adopting the above preferred scheme, since the washing liquid is relatively cheap, the addition and disposal of liquid can improve the washing effect, reduce the operation frequency of the transfer mechanism, reduce the conflict of the timing of various membrane reactions, and reduce the impact of the timing conflict on the test results after the rearrangement of the test results.

[0015] Furthermore, a slot for placing a reagent kit is provided on the receiving block of the sample placement transfer area, and a mechanism for taking and placing the reagent kit is provided on the chuck of the transfer unit.

[0016] Furthermore, it also includes a reagent storage area, which provides a low-temperature environment suitable for reagent storage, and the reagent storage area is provided with a warm bath block for placing the reagent kit; the transfer unit can drive the reagent kit to move to the reagent storage area and place the reagent kit on the warm bath block in the reagent storage area.

[0017] By adopting the above-mentioned preferred scheme, slots for placing the reagent kits are respectively provided in the sample placement transfer area and the reagent storage area, and a transfer unit is used to automatically move the reagent kit. After the reagent kit is used up, the reagent kit is automatically moved to the sample placement transfer area or the reagent storage area for temporary storage for recovery, thereby preventing the reagent kit from being placed in the reaction temperature zone for a long time and causing the reagent to become inactivated, thereby improving the reuse rate of the reagent kit for high-priced reagents and saving experimental costs.

[0018] Furthermore, the XYZ three-axis moving mechanism includes an X-axis moving mechanism, a Y-axis moving mechanism and a Z-axis moving mechanism, the base of the Z-axis moving mechanism is fixed on the base, the base of the Y-axis moving mechanism is installed on the moving block of the Z-axis moving mechanism, the base of the X-axis moving mechanism is installed on the moving block of the Y-axis moving mechanism, and the chuck is installed on the moving block of the X-axis moving mechanism.

[0019] Furthermore, the X-direction moving mechanism, the Y-direction moving mechanism and the Z-direction moving mechanism are provided with displacement sensors for detecting the movement positions of the chuck in the X-direction, Y-direction and Z-direction respectively.

[0020] By adopting the above preferred scheme, the transfer is more stable and the position accuracy is high. The small displacement action of the Z-axis moving mechanism can also realize repeated up and down oscillation of the sample membrane, which can promote the mixing and incubation of the test solution and the sample membrane and fully carry out the immune response.

[0021] Furthermore, the reaction temperature zone includes a bath block, a heating element, a heat dissipation element and a temperature sensor. The heating element is used to heat the bath block, the heat dissipation element is used to dissipate heat and cool the bath block, the temperature sensor is used to detect the temperature of the bath block, and the bath block is provided with a plurality of slots for placing the reagent kit.

[0022] By adopting the above preferred scheme, each reaction temperature zone can be set with a constant temperature condition according to the experimental conditions. In this way, once each reaction temperature zone reaches the set temperature, the temperature will no longer change, which reduces the waiting time required for temperature change and also reduces the energy waste caused by repeated heating and cooling.

[0023] Furthermore, the reagent storage area includes a warm bath block, a refrigeration plate, a heat dissipation element and a temperature sensor. The refrigeration plate is used to cool the warm bath block, the heat dissipation element is used to dissipate heat and cool the heating surface of the refrigeration plate, and the temperature sensor is used to detect the temperature of the warm bath block. The warm bath block is provided with a plurality of slots for placing the reagent kit.

[0024] The above preferred solution uses a refrigeration plate for refrigeration, which has a simple structure and can conveniently provide a stable low-temperature environment for the reagent.

[0025] Furthermore, the sample film clamp includes a main sheet body, a film pressing spring, a tensioning spring, an unlocking button and a cover plate, the main sheet body is a long strip sheet structure, the main sheet body is provided with a flat area for sample film attachment, the top end of the main sheet body is a clamping portion adapted to the clamp, a film pressing spring is installed between the clamping portion and the flat area, a rotating center axis is provided at the middle position of the film pressing spring, the lower end of the film pressing spring is a holding portion for holding the sample film, a tensioning spring is provided between the upper end of the film pressing spring and the main sheet body, and an unlocking button is provided on the cover plate at a position opposite to the upper end of the film pressing spring.

[0026] By adopting the above preferred solution, the sample film can be clamped and removed by only pressing the unlocking button, the sample film is convenient to clamp, and the sample film clip can be reused repeatedly.

[0027] A control system for a clip-on immunoblot analyzer, based on the above-mentioned clip-on immunoblot analyzer, comprising:

[0028] The test program input module is used by the test personnel to input the test steps and set the reaction conditions;

[0029] A main control module receives information transmitted by the test program input module, processes it, and sends a timing signal to the driving module; it also includes a timing rearrangement submodule, when the test program input module transmits test information again, the timing rearrangement submodule integrates the new test information and the timing that has not been implemented by the driving module to rearrange the timing, and transmits the rearranged timing to the driving module again;

[0030] The driving module sends out a command signal to control the movement of the XYZ three-axis moving mechanism and the picking and placing action of the chuck according to the timing signal transmitted by the main control module.

[0031] During the current sample membrane analysis process, the present invention can insert a new sample membrane analysis program at any time to rearrange the timing. When encountering a timing conflict, the conflicting timing can be rearranged in order according to the priority level set in the reaction stage. Since the sample membrane transfer action time is short, even if the timing is rearranged after the timing conflict, it will not affect the experimental results, and the new sample membrane can be inserted immediately for immunoblotting reaction, which greatly improves the convenience of experimental operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0033] Figure 1 It is a structural schematic diagram of an implementation mode of the present invention;

[0034] Figure 2 is a schematic structural diagram of a sample film clip in one embodiment of the present invention;

[0035] Figure 3 is a schematic structural diagram of a sample film clip in one embodiment of the present invention;

[0036] Figure 4 It is a structural schematic diagram of the control system of the present invention.

[0037] The numbers and letters in the figure represent the names of the corresponding parts:

[0038] 1-base; 2-sample membrane clamp; 21-main body; 22-film pressing spring; 23-tensioning spring; 24-unlocking button; 25-cover plate; 26-sample membrane; 3-sample placement transfer area; 4-reaction temperature zone; 5-chuck; 6-XYZ three-axis moving mechanism; 61-X-axis moving mechanism; 62-Y-axis moving mechanism; 63-Z-axis moving mechanism; 7-reagent storage area. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] like Figure 1 As shown, a clip-on immunoblotting analyzer comprises:

[0041] Base 1;

[0042] A sample membrane clamp 2, which is used to clamp a sample membrane containing a protein or polypeptide to be analyzed;

[0043] A sample placement transfer area 3, which is provided with a receiving block for placing sample film clips to be analyzed or analyzed;

[0044] Multiple reaction temperature zones 4, each of which provides temperature conditions suitable for the test steps, and each of which is provided with a temperature bath block for placing the reagent kit;

[0045] The transfer unit includes a chuck 5 and an XYZ three-axis moving mechanism 6. The chuck 5 is provided with a mechanism for taking out the sample membrane clip 2. The fixed end of the XYZ three-axis moving mechanism 6 is installed on the base 1. The chuck 5 is installed on the end moving block of the XYZ three-axis moving mechanism 6. The XYZ three-axis moving mechanism 6 drives the chuck 5 to move between the sample transfer zone 3 and each reaction temperature zone 4.

[0046] The beneficial effects of adopting the above technical scheme are: placing the test kit required for the test in the reaction temperature zone, clamping the sample membrane to be analyzed by the sample membrane clamp, and placing the sample membrane clamp in the sample placement transfer zone, and the transfer unit automatically clamps the sample membrane clamp from the sample placement transfer zone to the test kit in each reaction temperature zone for reaction; when a new sample membrane needs to be tested and analyzed, it is only necessary to clamp the new sample membrane to another sample membrane clamp and then directly place it in the sample placement transfer zone, and the transfer unit will automatically move the new sample membrane clamp into the reaction temperature zone for reaction according to the test steps; the immunoblot analyzer can insert new sample membranes for reaction at any time, and the test steps and conditions of each sample membrane can be set separately, which is more suitable for the test operation of laboratory personnel, greatly improving the use efficiency of the immunoblot analyzer, reducing queuing waiting time, and increasing the test speed.

[0047] In some other embodiments of the present invention, a washing area is further included, the washing area includes a washing box, a washing liquid adding mechanism and a washing liquid discarding mechanism, the washing liquid adding mechanism is used to add washing liquid into the washing box, and the washing liquid discarding mechanism is used to discharge the waste liquid in the washing box; the transfer unit can drive the sample membrane clip to move to the washing area and place the sample membrane clip into the washing box. The beneficial effects of adopting the above technical solution are: since the washing liquid is relatively cheap, the use of liquid adding and liquid discarding methods can improve the washing effect, reduce the operation frequency of the transfer mechanism, reduce the conflict of the reaction sequence of multiple membranes, and reduce the impact of the timing conflict on the test results after the rearrangement of the test sequence.

[0048] In other embodiments of the present invention, the receiving block in the sample transfer area is further provided with a slot for placing the reagent kit, and the chuck of the transfer unit is further provided with a mechanism for taking and placing the reagent kit. The specific forms of the mechanism for taking and placing the sample film clip and the mechanism for taking and placing the reagent kit on the chuck are not limited, and can be a mechanical clamp type or an electromagnetic magnetic suction type. Figure 1As shown, it also includes a reagent storage area 7, which provides a low-temperature environment suitable for reagent storage. The reagent storage area 7 is provided with a warm bath block for placing the reagent kit; the transfer unit can drive the reagent kit to move to the reagent storage area 7, and place the reagent kit on the warm bath block in the reagent storage area. The beneficial effects of the above technical solution are: a slot for placing the reagent kit is respectively provided in the sample transfer area and the reagent storage area, and the transfer unit is used to automatically realize the movement of the reagent kit. After the reagent kit is used, it is automatically moved to the sample transfer area or the reagent storage area for temporary storage for recycling, so as to prevent the reagent kit from being placed in the reaction temperature zone for a long time and the reagent from being inactivated, thereby improving the reuse rate of the reagent kit of the high-priced reagent and saving the experimental cost.

[0049] like Figure 1 As shown, in some other embodiments of the present invention, the XYZ three-axis moving mechanism 6 includes an X-direction moving mechanism 61, a Y-direction moving mechanism 62 and a Z-direction moving mechanism 63, the base of the Z-direction moving mechanism 63 is fixed on the base 1, the base of the Y-direction moving mechanism 62 is installed on the moving block of the Z-direction moving mechanism 63, the base of the X-direction moving mechanism 61 is installed on the moving block of the Y-direction moving mechanism 62, and the chuck 5 is installed on the moving block of the X-direction moving mechanism 61. The specific structures of the X-direction moving mechanism 61, the Y-direction moving mechanism 62 and the Z-direction moving mechanism 63 are not limited, and a motor can be used to drive a lead screw pair, or a motor can be used to drive a transmission wheel and a transmission belt to rotate. The X-direction moving mechanism, the Y-direction moving mechanism and the Z-direction moving mechanism are provided with displacement sensors for detecting the X, Y and Z moving positions of the chuck respectively. The beneficial effects of adopting the above technical solution are: the transfer is more stable, the positioning accuracy is high, and the small displacement action of the Z-axis moving mechanism can also realize repeated up and down oscillation of the sample membrane, which can promote the mixing and incubation of the test solution and the sample membrane to fully carry out the immune response.

[0050] In other embodiments of the present invention, the reaction temperature zone includes a bath block, a heating element, a heat dissipation element and a temperature sensor, the heating element is used to heat the bath block, the heat dissipation element is used to dissipate heat and cool the bath block, the temperature sensor is used to detect the temperature of the bath block, and the bath block is provided with a plurality of slots for placing the reagent kit. The beneficial effect of adopting the above technical solution is that each reaction temperature zone can be set to a constant temperature condition according to the test conditions, so that each reaction temperature zone will no longer change temperature once it reaches the set temperature, which reduces the waiting time required for temperature change and also reduces the energy waste caused by repeated temperature rise and fall.

[0051] In other embodiments of the present invention, the reagent storage area includes a warm bath block, a cooling sheet, a heat dissipation element and a temperature sensor, the cooling sheet is used to cool the warm bath block, the heat dissipation element is used to dissipate heat and cool the heating surface of the cooling sheet, the temperature sensor is used to detect the temperature of the warm bath block, and the warm bath block is provided with a plurality of slots for placing the reagent kit. The beneficial effect of adopting the above technical solution is: using the cooling sheet for cooling, the structure is simple, and a stable low-temperature environment can be conveniently provided for the reagent.

[0052] like Figure 2 , 3 As shown, in some other embodiments of the present invention, the sample film clamp 2 includes a main sheet body 21, a film pressing spring 22, a tensioning spring 23, an unlocking button 24 and a cover plate 25. The main sheet body 21 is a long strip sheet structure, and a plane area for attaching the sample film 26 is provided on the main sheet body 21. The top end of the main sheet body 21 is a clamping portion adapted to the chuck 5, and a film pressing spring 22 is installed between the clamping portion and the plane area. A rotation center axis is provided in the middle position of the film pressing spring 22, and the lower end of the film pressing spring 22 is a holding portion for holding the sample film 26. A tensioning spring 23 is arranged between the upper end of the film pressing spring 22 and the main sheet body 21, and an unlocking button 24 is arranged on the cover plate 25 at a position opposite to the upper end of the film pressing spring 22. The beneficial effect of the above technical solution is that the sample film can be clamped and removed by pressing the unlock button 24. After releasing the unlock button 24, the top of the sample film is fixed, the sample film is easy to clamp, and the sample film clip can be reused repeatedly. When clamped in this way, the sample film will be adsorbed on the surface of the main sheet due to the surface tension of the liquid after being separated from the test liquid, and the sample film will be separated from the surface of the main sheet after being immersed in the test liquid (except for the upper clamping and fixing position), so that the sample film will be fully in contact with the test liquid, improving the reaction effect.

[0053] like Figure 4 As shown, a control system of a clip-on immunoblot analyzer is based on the above-mentioned clip-on immunoblot analyzer, and comprises:

[0054] The test program input module is used by the test personnel to input the test steps and set the reaction conditions;

[0055] A main control module receives information transmitted by the test program input module, processes it, and sends a timing signal to the driving module; it also includes a timing rearrangement submodule, when the test program input module transmits test information again, the timing rearrangement submodule integrates the new test information and the timing that has not been implemented by the driving module to rearrange the timing, and transmits the rearranged timing to the driving module again;

[0056] The driving module sends out a command signal to control the movement of the XYZ three-axis moving mechanism and the picking and placing action of the chuck according to the timing signal transmitted by the main control module.

[0057] The beneficial effect of adopting the above technical solution is: during the current sample membrane analysis process, a new sample membrane analysis program can be inserted at any time to rearrange the timing. When encountering a timing conflict, the conflicting timing can be rearranged in order according to the priority level set according to the reaction stage. Since the sample membrane transfer action time is short, even if the timing is rearranged after the timing conflict, it will not affect the experimental results, and the new sample membrane can be inserted immediately for immunoblotting reaction, which greatly improves the convenience of experimental operation.

[0058] The following describes the operation process of one embodiment of the clip-on immunoblotting analyzer in combination with an immunoblotting experiment process of a biotin-labeled secondary antibody.

[0059] Western blot experiment flow chart for biotinylated secondary antibodies:

[0060]

[0061] Operation and action process of clip-on immunoblot analyzer:

[0062] 1. Add enough No. 1 1×TBST washing solution into the washing solution cylinder in the washing and liquid adding mechanism;

[0063] 2. Clamp the sample film onto the sample film clamp;

[0064] 3. In the test program input module, input the test steps and the corresponding reaction time and temperature. The main control module prompts to place the sample film clamp with the sample film in the corresponding slot of the sample placement transfer area according to the input test information, and prompts to place the reagent kits containing No. 1 solution, No. 2 solution, and No. 4-7 solutions in the corresponding slots of the reagent storage area;

[0065] 4. After the sample membrane clip and the reagent kit are placed in place, the immunoblot analyzer raises the temperature of a reaction temperature zone to 30°C, and automatically transfers the sample membrane clip between the washing zone and the reaction temperature zone according to the reaction program. If necessary, the required reagent kit is transferred from the reagent storage area to the reaction temperature zone in advance to increase the temperature. After the relevant reaction is completed, the reagent kit is transferred to the reagent storage area until the test is completed according to the reaction program; during the reaction, in order to improve the adequacy of the reaction, the Z-direction moving mechanism is used to drive the sample membrane clip to oscillate up and down for several rounds at intervals;

[0066] 5. When a control test is required, the sample film can be clamped onto another sample film clip immediately. In the test program input module, the control test steps and the corresponding reaction time and temperature are input. The main control module rearranges the reaction sequence according to the input test information, and prompts to place the sample film clip with the sample film in the corresponding slot of the sample placement transfer area, and prompts to place the reagent kit containing No. 3 solution or other reagent kits that need to be added in the corresponding slot of the reagent storage area (when there is a timing conflict between the two test programs, only the conflicting timing of the transfer mechanism action will be fine-tuned. If there is a situation where the same type of reagent kits cannot be used together, a prompt message that the reagent kit needs to be added will be issued in advance);

[0067] 6. After the new sample membrane clip and reagent kit are in place, the immunoblot analyzer continues according to the rearranged sequence until the reaction is completed.

[0068] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable ordinary technicians in the field to understand the content of the present invention and implement it. They cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A clamp-on immunoblot analyzer, characterized in that: include: Base; A sample membrane clamp, which is used to clamp a sample membrane containing a protein or polypeptide to be analyzed; A sample placement transfer area, which is provided with a receiving block for placing sample film clips to be analyzed or analyzed; Multiple reaction temperature zones, each of which provides temperature conditions that match the test steps, and each reaction temperature zone is equipped with a temperature bath block for placing the reagent kit; A transfer unit, comprising a chuck and an XYZ three-axis moving mechanism, wherein the chuck is provided with a mechanism for taking and placing the sample film clip, the fixed end of the XYZ three-axis moving mechanism is mounted on the base, the chuck is mounted on the end moving block of the XYZ three-axis moving mechanism, and the XYZ three-axis moving mechanism drives the chuck to move between the sample placement transfer zone and each reaction temperature zone; the XYZ three-axis moving mechanism comprises an X-direction moving mechanism, a Y-direction moving mechanism and a Z-direction moving mechanism, the base of the Z-direction moving mechanism is fixed on the base, the base of the Y-direction moving mechanism is mounted on the moving block of the Z-direction moving mechanism, the base of the X-direction moving mechanism is mounted on the moving block of the Y-direction moving mechanism, and the chuck is mounted on the moving block of the X-direction moving mechanism; The sample film clamp comprises a main sheet body, a film pressing spring, a tensioning spring, an unlocking button and a cover plate, wherein the main sheet body is a long strip sheet body structure, a plane area for sample film attachment is provided on the main sheet body, the top end of the main sheet body is a clamping portion adapted to the clamp head, a film pressing spring is installed between the clamping portion and the plane area, a rotating central axis is provided at the middle position of the film pressing spring, the lower end of the film pressing spring is a holding portion for holding the sample film, a tensioning spring is provided between the upper end of the film pressing spring and the main sheet body, and an unlocking button is provided on the cover plate at a position directly opposite to the upper end of the film pressing spring; It also includes a control system, which includes: The test program input module is used by the test personnel to input the test steps and set the reaction conditions; A main control module receives information transmitted by the test program input module, processes it, and sends a timing signal to the driving module; it also includes a timing rearrangement submodule, when the test program input module transmits test information again, the timing rearrangement submodule integrates the new test information and the timing that has not been implemented by the driving module to rearrange the timing, and transmits the rearranged timing to the driving module again; The driving module sends out a command signal to control the movement of the XYZ three-axis moving mechanism and the picking and placing action of the chuck according to the timing signal transmitted by the main control module.

2. The clip-on immunoblot analyzer according to claim 1, characterized in that: It also includes a washing area, which includes a washing box, a washing liquid adding mechanism and a washing liquid discarding mechanism, wherein the washing liquid adding mechanism is used to add washing liquid into the washing box, and the washing liquid discarding mechanism is used to discharge waste liquid in the washing box; The transfer unit can drive the sample film clip to move to the washing area and place the sample film clip into the washing box.

3. The clip-on immunoblot analyzer according to claim 1, characterized in that: A slot for placing a reagent box is also provided on the receiving block of the sample placement transfer zone, and a mechanism for taking and placing the reagent box is also provided on the clamping head of the transfer unit.

4. The clip-on immunoblot analyzer according to claim 3, characterized in that: It also includes a reagent storage area, which provides a low-temperature environment suitable for reagent storage. The reagent storage area is provided with a warm bath block for placing the reagent kit; the transfer unit can drive the reagent kit to the reagent storage area and place the reagent kit on the warm bath block in the reagent storage area.

5. The clip-on immunoblot analyzer according to claim 1, characterized in that: The X-direction moving mechanism, the Y-direction moving mechanism and the Z-direction moving mechanism are provided with displacement sensors for respectively detecting the moving positions of the clamp in the X-direction, Y-direction and Z-direction.

6. The clip-on immunoblot analyzer according to claim 1, characterized in that: The reaction temperature zone includes a bath block, a heating element, a heat dissipation element and a temperature sensor. The heating element is used to heat the bath block, the heat dissipation element is used to dissipate heat and cool the bath block, the temperature sensor is used to detect the temperature of the bath block, and the bath block is provided with a plurality of slots for placing the reagent kit.

7. The clip-on immunoblot analyzer according to claim 4, characterized in that: The reagent storage area includes a warm bath block, a refrigeration plate, a heat dissipation element and a temperature sensor. The refrigeration plate is used to cool the warm bath block, the heat dissipation element is used to dissipate heat and cool the heating surface of the refrigeration plate, and the temperature sensor is used to detect the temperature of the warm bath block. The warm bath block is provided with a plurality of slots for placing reagent kits.

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