A calibration device for microbial identification and drug sensitivity analysis system

Abstract

The present invention discloses a microbial identification and drug sensitivity analysis system calibration device, including a calibration component and a charging component; the calibration component includes a calibration component control circuit, a charging interface male head, a control switch and a plurality of temperature optical calibration units; the charging component includes an electrical housing, a charging interface female head, a housing charging indicator light, a charging housing charging indicator light, a USB interface, a charging housing switch and a charging component control circuit. The microbial identification and drug sensitivity analysis system calibration device of the present invention can replace known bacterial genus quality control strains as a microbial identification and drug sensitivity analysis system calibration medium, and its performance is stable and reliable. In the calibration operation, it is not necessary to prepare a known bacterial genus quality control strain positive culture solution and a large number of drug sensitivity identification plates, and the use cost is low. The cause of false negatives in the microbial identification and drug sensitivity analysis system can be effectively discovered, and the synchronous calibration of temperature parameters and optical parameters can be realized, which saves calibration time and has a wide range of applications.

Description

Technical Field

[0001] The invention belongs to the technical field of quality control of microbial identification and drug sensitivity analysis systems, and in particular relates to a microbial identification and drug sensitivity analysis system calibration device. Background Art

[0002] The microbial identification and antimicrobialsusceptibility testing system (hereinafter referred to as the system) is an instrument that can perform bacterial identification and antimicrobial susceptibility testing at the same time. The system uses traditional photoelectric colorimetry and rapid fluorescence methods for bacterial identification, and uses photoelectric turbidimetry and fluorescence determination methods for antimicrobial susceptibility testing. When the system is working, the constant temperature circuit in the incubator is controlled by the CPU to maintain a constant temperature. There is a photoelectric reader in the box. The monochromatic light emitted by the optical path part scans each well of each test card. The color, turbidity or fluorescence intensity changes caused by the decomposition or utilization of the substrate by bacteria are determined by photoelectric colorimetry, photoelectric turbidimetry or fluorescence. The absorbance or fluorescence intensity value of the change is converted into an electrical signal by the reader, and then analyzed and calculated by the microcomputer.

[0003] According to CNAS-CL02:2012 "Quality and Competence Accreditation Criteria for Medical Laboratories" (ISO15189:2012, IDT), calibration of microbial identification and antimicrobial susceptibility analysis systems is necessary. Although the calibration of microbial identification and antimicrobial susceptibility analysis systems can refer to YY / T 1531-2017 "Bacterial Biochemical Identification System" which uses temperature sensors to test accuracy and uses known bacterial quality control strains to conduct positive tests, it is impossible to detect possible false negatives in the system. At the same time, there is a lack of performance evaluation methods for the temperature difference between the internal wells of the system incubation module and the optical system, and the calibration method is very imperfect. At the same time, CLSIM52 "Verification of Commercial Microbial Identification and Antimicrobial Susceptibility Testing Systems" requires the evaluation and functional verification of the constant temperature incubator and photoelectric reader of the microbial identification and antimicrobial susceptibility analysis system, but there is currently a lack of corresponding dedicated testing equipment to carry out. Summary of the invention

[0004] The purpose of the present invention is to provide a microbial identification and drug sensitivity analysis system calibration device, which uses the integration of temperature sensors and optical sensors to monitor the changes in temperature and optical parameters during the operation of the microbial identification and drug sensitivity analysis system in real time to simultaneously calibrate the temperature parameters and optical parameters of the system; it solves the problems of complex positive test procedures using known bacterial quality control strains and inability to detect false negatives, and the inability to synchronize the calibration of the temperature system with the positive test of known bacterial quality control strains, as well as the lack of optical system calibration methods.

[0005] To achieve the above object, the present invention adopts the following technical solution:

[0006] A microbial identification and drug sensitivity analysis system calibration device, comprising a calibration component and a charging component;

[0007] The calibration component includes a calibration component control circuit, a charging interface male plug, a control switch and a plurality of temperature optical calibration units;

[0008] The temperature optical calibration unit includes a coupled temperature sensor and an optical sensor, wherein the temperature sensor is arranged at the bottom of the calibration component and is used to monitor the temperature signal; the optical sensor includes one or more silicon photocells and is used to monitor the emitted light emitted by the light source of the microbial identification and drug sensitivity analysis system in a direction perpendicular to the drug sensitivity identification plate;

[0009] The calibration component control circuit is used to control the temperature sensor and the optical sensor to perform signal monitoring and storage;

[0010] The male charging interface matches the female charging interface of the charging assembly and is used for integrated charging of the calibration device;

[0011] The control switch is used to control the on and off state of the calibration component;

[0012] The charging assembly includes an electrical housing, a charging interface female head, a housing charging indicator light, a charging housing charging indicator light, a USB interface, a charging housing switch and a charging assembly control circuit;

[0013] The structure of the charger housing matches the structure of the calibration component and is used to place the calibration component for charging;

[0014] The female charging interface matches the male charging interface of the calibration component, and the calibration component is inserted into the charging component for integrated charging;

[0015] The housing charging indicator light is used to indicate whether the calibration component is charged normally after being placed in the housing;

[0016] The charging indicator light of the charging housing is used to indicate whether the charging component is powered normally after being connected to an external power source;

[0017] The USB interface is used for charging with an external power source;

[0018] The charging housing switch is used to control the on and off state of the charging assembly;

[0019] The charging component control circuit is used to control the charging process of the charging component.

[0020] Preferably, the calibration component further includes a working indicator light, a lithium battery and an antenna;

[0021] The working indicator light is used to indicate the working status of the calibration component;

[0022] The lithium battery is used to provide the energy required for the normal endurance of the calibration component;

[0023] The antenna is coupled to the calibration component control circuit and is used to wirelessly transmit the temperature and optical data stored in the calibration component control circuit to the mobile phone APP end, and the transmitted data information is used for subsequent evaluation of the calibration results.

[0024] Preferably, the temperature optical calibration units are symmetrically distributed at the edge and central area of ​​the location where the drug sensitivity identification plate of the microbial identification and drug sensitivity analysis system is placed.

[0025] Preferably, a cosine corrector is provided in the optical sensor to eliminate optical interface problems in the light collection and sampling geometry of the optical sensor of the calibration unit.

[0026] Preferably, the temperature sensor and the optical sensor are arranged on opposite surfaces of the calibration component.

[0027] Preferably, the calibration component control circuit automatically stores records according to proportional changes in the temperature signal detected by the temperature sensor and the optical signal detected by the optical sensor.

[0028] Preferably, the calibration component control circuit includes a signal conditioning module, an analog / digital conversion module, a programmable amplifier, a main controller, a Bluetooth communication module and a lithium battery conversion module;

[0029] The signal conditioning module corresponds to the temperature sensor and the optical sensor and is used for collecting temperature signals and optical signals;

[0030] The analog / digital conversion module is used for analog-to-digital conversion processing of the collected signal;

[0031] The program-controlled amplifier is controlled by instructions sent by a mobile phone APP and is used to amplify optical signals;

[0032] The main controller is used for processing temperature signals and optical signals;

[0033] The Bluetooth communication module is used for wireless transmission of data;

[0034] The lithium battery conversion module is used to provide the electric energy required by the calibration component control circuit.

[0035] Preferably, the calibration component is a plate-like structure with a minimum length of 91 mm, a width of 56.8 mm, and a thickness of 4 mm, which is consistent with the size of clinical drug sensitivity identification plates, and is configured with inlaid sets of different sizes, which are inlaid on the outside of the calibration component to match the position of the drug sensitivity identification plates placed inside the microbial identification and drug sensitivity analysis systems of different manufacturers and models during calibration.

[0036] Preferably, the charging component control circuit includes a power input conversion module and a battery output charging module;

[0037] The power input conversion module includes a USB input interface, a charging chip and a lithium battery;

[0038] The USB input interface is connected to a 5V voltage, and the charging chip is used to charge the internal lithium battery;

[0039] The battery output charging module includes a conversion control switch, a boost module, a charging chip and a charging output interface;

[0040] The conversion control switch converts the charging board function into an output function to charge the calibration component;

[0041] The boost module increases the voltage of the lithium battery to 5V;

[0042] The charging chip and the charging output interface are used to charge the lithium battery of the calibration component.

[0043] The present invention has the following advantages:

[0044] The microbial identification and drug sensitivity analysis system calibration device of the present invention can replace known bacterial quality control strains as the microbial identification and drug sensitivity analysis system calibration medium, and has stable and reliable performance. During the calibration operation, it is not necessary to prepare positive culture fluids of known bacterial quality control strains and a large number of drug sensitivity identification plates. The use cost is low, and the cause of false negatives in the microbial identification and drug sensitivity analysis system can be effectively discovered. The synchronous calibration of temperature parameters and optical parameters can be realized, thus saving calibration time and having a wide range of applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 It is a block diagram of the design principle of an embodiment of the present invention;

[0046] Figure 2 A schematic diagram of a structural model of an embodiment of the present invention;

[0047] Among them, 1- charging interface male head, 2- temperature optical calibration unit, 3- shell charging indicator light, 4- working indicator light, 5- charging shell charging indicator light, 6- USB interface, 7- control switch, 8- charging shell switch, 9- charger shell, 10- charging interface female head;

[0048] Figure 3 It is a principle block diagram of a control circuit of a calibration component according to an embodiment of the present invention;

[0049] Figure 4 This is a principle block diagram of a charging component control circuit according to an embodiment of the present invention. DETAILED DESCRIPTION

[0050] The following is a description of the implementation of the present invention by means of specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0051] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention, so they have no substantial technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the present invention without affecting the effects and purposes that can be achieved by the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle", etc. cited in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of the present invention without substantially changing the technical content.

[0052] Example 1

[0053] like Figure 1 and Figure 2 As shown, a microbial identification and drug sensitivity analysis system calibration device of the present invention includes a calibration component and a charging component;

[0054] In the embodiment, the calibration component is a plate-like structure with a minimum length of 91 mm, a width of 56.8 mm, and a thickness of 4 mm, which is consistent with the size of the clinical drug sensitivity identification plate, and is configured with inlay plates of different sizes, which are inlaid on the outside of the calibration component to match the position of the drug sensitivity identification plate placed inside the microbial identification and drug sensitivity analysis system of different manufacturers and models during calibration.

[0055] The calibration component includes a calibration component control circuit, a charging interface male connector 1, a control switch 7 and five temperature optical calibration units 2;

[0056] In the embodiment, the temperature optical calibration units 2 are symmetrically distributed at the edge and central area of ​​the microbial identification and drug sensitivity analysis system where the drug sensitivity identification plate is placed, and the calibration does not affect the normal working state of the microbial identification and drug sensitivity analysis system.

[0057] The temperature optical calibration unit 2 includes a temperature sensor and an optical sensor coupled;

[0058] The temperature sensor is arranged at the bottom of the calibration component to monitor the temperature signal; the optical sensor includes one or more silicon photocells to monitor the emitted light emitted by the light source of the microbial identification and drug sensitivity analysis system in a direction perpendicular to the drug sensitivity identification plate;

[0059] When the microbial identification and drug sensitivity analysis system calibration device is placed in the position of the microbial identification and drug sensitivity analysis system for placing the drug sensitivity identification plate, the temperature signal and optical signal at the position where the drug sensitivity identification plate is placed are monitored by the temperature sensors and optical sensors of different calibration units on the microbial identification and drug sensitivity analysis system calibration device according to the temperature changes of the temperature sensor and the optical changes monitored by the optical sensor. The changes in temperature parameters and optical parameters of the microbial identification and drug sensitivity analysis system after being placed in the microbial identification and drug sensitivity analysis system calibration device can be read through a mobile phone APP.

[0060] In an embodiment, a cosine corrector is provided in the optical sensor, and a calibration unit of the calibration component collects radiation (light) within a 180° solid angle, thereby eliminating optical interface problems in the light collection sampling geometry of the optical sensor of the calibration unit.

[0061] In the embodiment, the temperature sensor and the optical sensor are arranged on opposite surfaces of the calibration component. The measurement range of the temperature sensor is (0-50)°C, and the maximum allowable error is ±0.1°C; the wavelength range of the optical sensor is (360-720)nm, the illumination is (0-200000)lx, and the maximum allowable error is ±5%.

[0062] When the calibration device of the microbial identification and drug sensitivity analysis system is working, the five calibration units on the calibration component monitor in real time the temperature and optical parameters of the five drug sensitivity identification plates in different positions of the microbial identification and drug sensitivity analysis system. Each calibration unit consists of a coupled temperature sensor and an optical sensor, which are located on opposite surfaces of the calibration component. During the operation of the microbial identification and drug sensitivity analysis system, the temperature of the incubation module needs to be kept constant, and the light source output signal of the system changes periodically.

[0063] The calibration component control circuit is used to control the temperature sensor and the optical sensor to perform signal monitoring and storage;

[0064] In an embodiment, the calibration component control circuit automatically stores records according to proportional changes in the temperature signal detected by the temperature sensor and the optical signal detected by the optical sensor.

[0065] The male charging interface 1 matches the female charging interface 10 of the charging assembly and is used for integrated charging of the calibration device;

[0066] The control switch 7 is used to control the on and off state of the calibration component;

[0067] The charging assembly includes an electrical housing 9, a charging interface female head 10, a housing charging indicator light 3, a charging housing charging indicator light 5, a USB interface 6, a charging housing switch 8 and a charging assembly control circuit;

[0068] The structure of the charger housing 9 matches the structure of the calibration component and is used to place the calibration component for charging;

[0069] The charging interface female connector 10 matches the charging interface male connector 1 of the calibration component, and the calibration component is inserted into the charging component for integrated charging;

[0070] The housing charging indicator light 3 is used to indicate whether the calibration component is charged normally after being placed in the housing;

[0071] The charging indicator light 5 of the charging housing is used to indicate whether the charging component is powered normally after being connected to an external power source;

[0072] The USB interface 6 is used for charging with an external power source;

[0073] The charging housing switch 8 is used to control the on and off state of the charging assembly;

[0074] The charging component control circuit is used to control the charging process of the charging component.

[0075] In the embodiment, the calibration component further includes a working indicator light 4, a lithium battery and an antenna;

[0076] The working indicator light 4 is used to indicate the working status of the calibration component;

[0077] The lithium battery is used to provide the energy required for the normal endurance of the calibration component;

[0078] The antenna is coupled to the calibration component control circuit and is used to wirelessly transmit the temperature and optical data stored in the calibration component control circuit to the mobile phone APP end, and the transmitted data information is used for subsequent evaluation of the calibration results.

[0079] like Figure 3As shown, in the embodiment, the calibration component control circuit includes a signal conditioning module, an analog / digital conversion module, a programmable amplifier, a main controller, a Bluetooth communication module and a lithium battery conversion module;

[0080] The signal conditioning module corresponds to the temperature sensor and the optical sensor and is used for collecting temperature signals and optical signals;

[0081] The analog / digital conversion module is used for analog-to-digital conversion processing of the collected signal;

[0082] The program-controlled amplifier is controlled by instructions sent by a mobile phone APP and is used to amplify optical signals;

[0083] The main controller is used for processing temperature signals and optical signals;

[0084] The Bluetooth communication module is used for wireless transmission of data;

[0085] The lithium battery conversion module is used to provide the electric energy required by the calibration component control circuit.

[0086] In the embodiment, the optical sensor outputs a current signal, which is converted into a voltage signal by a signal conditioning module and directly input into an STM32 single-chip computer. The built-in programmable amplifier of the STM32F3 series is used to amplify the voltage representing the optical signal by 2, 4, 8, or 16 times, and the operation can be regulated by sending instructions by a mobile phone APP. The temperature sensor inputs the output voltage signal differentially into an analog / digital conversion module for analog-to-digital conversion, and can amplify the voltage signal, and then communicates to the main control single-chip computer through an SPI interface. After sorting and calculating, the temperature data is transferred to the Bluetooth communication module for transmission.

[0087] like Figure 4 As shown, in the embodiment, the charging component control circuit includes a power input conversion module and a battery output charging module;

[0088] The power input conversion module includes a USB input interface, a charging chip and a lithium battery;

[0089] The USB input interface is connected to a 5V voltage, and the charging chip is used to charge the internal lithium battery;

[0090] The battery output charging module includes a conversion control switch, a boost module, a charging chip and a charging output interface;

[0091] The conversion control switch converts the charging board function into an output function to charge the calibration component;

[0092] The boost module increases the voltage of the lithium battery to 5V;

[0093] The charging chip and the charging output interface are used to charge the lithium battery of the calibration component.

[0094] Although the present invention has been described in detail above by general description and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements can be made to the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A microbial identification and drug sensitivity analysis system calibration device, characterized in that: Including calibration components and charging components, The calibration component comprises a calibration component control circuit, a charging interface male connector (1), a control switch (7) and a plurality of temperature optical calibration units (2); the temperature optical calibration unit (2) comprises a coupled temperature sensor and an optical sensor, the temperature sensor being arranged at the bottom of the calibration component for monitoring temperature signals; the optical sensor comprises one or more silicon photocells, which are used to calibrate the optical interface problem in the optical sensor light collection sampling geometry through the cosine corrector provided thereon, and monitor the emitted light emitted by the light source of the microbial identification and drug sensitivity analysis system in a direction perpendicular to the drug sensitivity identification plate; the calibration component control circuit is used to control the temperature sensor and the optical sensor for signal monitoring and storage; the charging interface male connector (1) matches the charging interface female connector (10) of the charging component for integrated charging of the calibration device; the control switch (7) is used to control the on and off state of the calibration component; The charging assembly comprises a charger housing (9), a charging interface female head (10), a housing charging indicator light (3), a charging housing charging indicator light (5), a USB interface (6), a charging housing switch (8) and a charging assembly control circuit; the structure of the charger housing (9) matches the structure of the calibration assembly and is used to place the calibration assembly for charging; the charging interface female head (10) matches the charging interface male head (1) of the calibration assembly, and the calibration assembly is inserted into the charging assembly for integrated charging; the housing charging indicator light (3) is used to indicate whether the calibration assembly is charged normally after being inserted; the charging housing charging indicator light (5) is used to indicate whether the charging assembly is powered on normally after being connected to an external power source; the USB interface (6) is used to charge with an external power source; the charging housing switch (8) is used to control the on and off state of the charging assembly; and the charging assembly control circuit is used to control the charging process of the charging assembly.

2. A microbial identification and drug sensitivity analysis system calibration device according to claim 1, characterized in that: The calibration component also includes a working indicator light (4), a lithium battery and an antenna; the working indicator light (4) is used to indicate the working status of the calibration component; the lithium battery is used to provide energy required for the normal endurance of the calibration component; the antenna is coupled to the calibration component control circuit and is used to wirelessly transmit the temperature and optical data stored in the calibration component control circuit to the mobile phone APP end, and the transmitted data information is used for subsequent evaluation of the calibration results.

3. A microbial identification and drug sensitivity analysis system calibration device according to claim 1, characterized in that: The temperature optical calibration units (2) are symmetrically distributed at the edge and central area of ​​the location where the drug sensitivity identification plate of the microbial identification and drug sensitivity analysis system is placed.

4. The microbial identification and drug sensitivity analysis system calibration device according to claim 1, characterized in that: The temperature sensor and the optical sensor are arranged on opposite surfaces of the calibration component.

5. The microbial identification and drug sensitivity analysis system calibration device according to claim 1, characterized in that: The calibration component control circuit automatically stores records according to proportional changes of the temperature signal detected by the temperature sensor and the optical signal detected by the optical sensor.

6. A microbial identification and drug sensitivity analysis system calibration device according to claim 1, characterized in that: The calibration component control circuit includes a signal conditioning module, an analog / digital conversion module, a programmable amplifier, a main controller, a Bluetooth communication module and a lithium battery conversion module; the signal conditioning module corresponds to the temperature sensor and the optical sensor, and is used for collecting temperature signals and optical signals; the analog / digital conversion module is used for analog-to-digital conversion processing of the collected signals; the programmable amplifier is regulated by instructions sent by a mobile phone APP, and is used for amplifying optical signals; the main controller is used for processing temperature signals and optical signals; the Bluetooth communication module is used for wireless transmission of data; and the lithium battery conversion module is used to provide the electrical energy required by the calibration component control circuit.

7. A microbial identification and drug sensitivity analysis system calibration device according to any one of claims 1 to 6, characterized in that: The calibration component is a plate-like structure with a minimum length of 91 mm, a width of 56.8 mm, and a thickness of 4 mm, which is consistent with the size of clinical drug sensitivity identification plates. It is equipped with inlaid sets of different sizes, which are inlaid on the outside of the calibration component to match the position of the drug sensitivity identification plates placed inside the microbial identification and drug sensitivity analysis systems of different manufacturers and models during calibration.

8. The microbial identification and drug sensitivity analysis system calibration device according to claim 1, characterized in that: The charging component control circuit includes a power input conversion module and a battery output charging module; the power input conversion module includes a USB input interface, a charging chip and a lithium battery; the USB input interface is connected to a 5V voltage, and the charging chip is used to charge the internal lithium battery; the battery output charging module includes a conversion control switch, a boost module, a charging chip and a charging output interface; the conversion control switch converts the charging board function into an output function to charge the calibration component; the boost module increases the lithium battery voltage to 5V; the charging chip and the charging output interface are used to charge the lithium battery of the calibration component.

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