processing device

Abstract

The present invention provides a processing device that can efficiently perform microwave irradiation. The processing device includes a container 101 made of a material having microwave reflectivity and having a first end portion 1015a and an irradiation opening portion 1013 that is an exit portion of microwaves irradiated into the interior; a first filter 105 disposed so as to divide the container 101 and separate solid objects from the contents in the container 101; and a first reflection member 106 disposed so as to divide the container 101 on the side of the first end portion 1015a from the exit portion, and allowing the contents to pass through at least the first filter 105 and reflecting microwaves.

Description

TECHNICAL FIELD

[0001] The present application relates to a processing apparatus used for solid phase synthesis and the like. BACKGROUND

[0002] A device used for peptide solid phase synthesis known in the prior art is one including a reaction tank having transparency to microwave irradiation, a passage for adding a liquid to the reaction tank, a passage for taking out a liquid from the reaction tank without taking out a solid, a microwave cavity for holding the reaction tank, and a microwave source in wave communication with the cavity (see, for example, Patent Document 1).

[0003] PRIOR ART DOCUMENTS

[0004] PATENT DOCUMENTS

[0005] Patent Document 1 Japanese Patent Application Laid-Open No. 2005-15483 (page 1, Fig. 1, etc.) SUMMARY

[0006] PROBLEMS TO BE SOLVED BY THE INVENTION

[0007] However, in the device used for peptide solid phase synthesis in the prior art as described above, in which a microwave irradiation device that generates a single mode microwave from the outside of a reaction tank having transparency to microwave irradiation, the single mode microwave is concentrated in a very narrow region, and thus it is difficult to efficiently irradiate the microwave. For example, it is not possible to uniformly irradiate the microwave in a container such as a reaction tank, and thus it is difficult to efficiently perform a process in the entire container. Therefore, for example, even if the reaction tank of the prior art device is simply enlarged, the microwave irradiation region is limited, and thus it is difficult to increase the process amount.

[0008] Further, in the device in the prior art as described above, in which a container used for solid phase synthesis is a reaction tank having transparency to microwave irradiation, the microwave irradiated into the reaction tank penetrates from the side surface, the upper portion, the bottom surface, and the like of the reaction tank to the outside, and thus it is difficult to confine the irradiated microwave in the reaction tank, and thus it is difficult to efficiently perform microwave irradiation in the container.

[0009] Further, when the device as described above is used for a process using a microwave other than peptide solid phase synthesis, the same problems occur.

[0010] As described above, the prior art has a problem in that it is difficult to efficiently perform microwave irradiation.

[0011] The present application has been achieved in order to solve the above problems, and has an object to provide a processing apparatus that can efficiently perform microwave irradiation.

[0012] TECHNICAL SOLUTION TO THE PROBLEMS

[0013] The processing device of the present application is provided with: a container made of a material having microwave reflectivity and having a first end portion and an exit portion for irradiating microwaves into the interior; a first filter disposed so as to divide the container and separating solid matter that is a separation target from the contents in the container; and a first reflection member disposed so as to divide the container on the first end portion side from the exit portion and allowing passage of the contents through at least the first filter and reflecting microwaves.

[0014] With this configuration, microwave irradiation can be efficiently performed. Also, by reflecting microwaves with the first reflection member, microwaves are less likely to be irradiated to the region on the first end portion side from the first filter for separating solid matter that is a separation target and on the first end portion side from the first reflection member, and microwave irradiation can be efficiently performed to regions where solid matter cannot be separated or where there is no solid matter, and so on.

[0015] Also, the processing device of the present application is as follows: in the processing device, the first reflection member is provided on the first end portion side from the first filter or between the first filter and the exit portion.

[0016] With this configuration, microwaves are less likely to be irradiated to the region on the first end portion side from the first reflection member, and microwave irradiation can be efficiently performed to regions where solid-phase resin or solid matter cannot be separated or where there is no solid matter, and so on.

[0017] Also, the processing device of the present application is as follows: in the processing device, the first filter and the first reflection member are disposed in an overlapping manner.

[0018] With this configuration, the first filter can be reinforced by the first reflection member. Thereby, for example, filter selectivity can be increased.

[0019] Also, the processing device of the present application is as follows: in the processing device, the first reflection member is disposed between the first filter and the exit portion and allows passage of the solid matter.

[0020] With this configuration, microwaves are less likely to be irradiated to the region on the first end portion side from the first reflection member, and microwave irradiation can be efficiently performed.

[0021] Also, the processing device of the present application is as follows: in the processing device, the first filter and the first reflection member are integrated, and a first reflection filter is configured, which is a filter that separates solid matter that is a separation target from the contents in the container and reflects microwaves.

[0022] With this configuration, the microwave is less likely to be radiated to a region where the solid matter cannot be separated or a region where the solid matter does not exist, i.e., a region on the first end portion side of the first reflection filter, and the microwave radiation can be efficiently performed. Also, the first filter is easily handled by being integrated with the first reflection member.

[0023] Also, the processing device of the present application is as follows: in the processing device, the container further has a second end portion, the exit portion is provided at a position between the first end portion and the second end portion of the container, and the processing device further includes: a second filter configured to separate the container on the second end portion side of the first filter and the first reflection member and to separate the solid matter that is the separation target from the contents in the container; and a second reflection member configured to separate the container on the second end portion side of the first filter and the exit portion and to allow the contents to pass through at least the second filter and to reflect the microwave.

[0024] With this configuration, the microwave is reflected by the first reflection member and the second reflection member, whereby the microwave is less likely to be radiated to: a region on the first end portion side of the first filter to separate the solid matter that is the separation target and a region on the first end portion side of the first reflection member; and a region on the second end portion side of the second filter to separate the solid matter that is the separation target and a region on the second end portion side of the second reflection member; the microwave is less likely to be radiated to a region where the solid matter cannot be separated or a region where the solid matter does not exist, and the microwave radiation can be efficiently performed.

[0025] Also, the processing device of the present application is as follows: in the processing device, the second reflection member is provided between the second filter and the exit portion on the second end portion side of the second filter.

[0026] With this configuration, the microwave is less likely to be radiated to a region on the second end portion side where the solid-phase resin or the solid matter cannot be separated or a region where the solid matter does not exist, and the microwave radiation can be efficiently performed.

[0027] Also, the processing device of the present application is as follows: in the processing device, the second filter is configured to overlap the second reflection member.

[0028] With this configuration, the second filter can be reinforced by the second reflection member. Thereby, for example, the selectivity of the second filter can be increased.

[0029] Also, the processing device of the present application is as follows: in the processing device, the second reflection member is configured between the second filter and the exit portion and allows the solid matter to pass through.

[0030] With this configuration, the microwave is less likely to be radiated to the area on the second end portion side of the second reflection member, and the microwave radiation can be efficiently performed.

[0031] Further, the processing device of the present application is as follows: in the processing device, the second filter is integrated with the second reflection member, and a second reflection filter is configured, which is a filter that separates the solid matter that is the separation object from the content in the container and reflects the microwave.

[0032] With this configuration, the microwave is less likely to be radiated to the area on the second end portion side of the second reflection filter where the solid matter cannot be separated or where the solid matter does not exist, and the microwave radiation can be efficiently performed. Further, the second filter and the second reflection member are integrated, and thus the operation is facilitated.

[0033] Further, the processing device of the present application is as follows: in the processing device, a first opening portion that performs at least one of the supply and discharge of the content is provided on the first end portion side of the container with respect to the first filter and the first reflection member.

[0034] With this configuration, the supply of the content into the container and the discharge of the content from the container can be performed by the first opening portion.

[0035] Further, the processing device of the present application is as follows: in the processing device, a first opening portion that performs at least one of the supply and discharge of the content is provided on the first end portion side of the container with respect to the first filter and the first reflection member, and a second opening portion that performs at least one of the supply and discharge of the content is provided on the second end portion side of the container with respect to the second filter and the second reflection member.

[0036] With this configuration, the supply of the content into the container and the discharge of the content from the container can be performed by the first opening portion and the second opening portion.

[0037] Further, the processing device of the present application is as follows: in the processing device, the first end portion is the lower end portion of the container, and the first opening portion is a discharge port that performs the discharge of the content.

[0038] With this configuration, the discharge of the content from the container can be performed by the first opening portion.

[0039] Further, the processing device of the present application is as follows: in the processing device, the microwave radiation from the emission portion is performed in a state where the content flows in the container between the first opening portion and the second opening portion.

[0040] With this configuration, the processing can be performed in a state where the content flows in the container, for example, the processing can be performed in a state where the material used for the reaction and the like continuously flow, and the like.

[0041] Further, the processing device of the present application is a processing device in which the following processes are performed: a process of supplying contents from the first opening portion and discharging the contents from the second opening portion, and a process performed inside the container; and a process of supplying contents from the second opening portion and discharging the contents from the first opening portion, and a process performed inside the container.

[0042] With this configuration, the direction in which contents are supplied into the container can be changed, and appropriate processing can be performed. For example, the direction in which contents are supplied can be changed in response to the processing.

[0043] Further, the processing device of the present application is a processing device in which the following processes are performed: a process of supplying contents from the first opening portion and discharging the contents from the second opening portion, and a process performed inside the container; and a process of supplying contents from the second opening portion and discharging the contents from the first opening portion, and a process performed inside the container.

[0044] With this configuration, the direction in which contents are supplied into the container can be changed, and appropriate processing can be performed. For example, the direction in which contents are supplied can be changed in response to the processing.

[0045] Further, the processing device of the present application is a processing device in which the following processes are performed: a process of supplying contents from the first opening portion and discharging the contents from the second opening portion, and a process performed inside the container; and a process of supplying contents from the second opening portion and discharging the contents from the first opening portion, and a process performed inside the container.

[0046] With this configuration, the direction in which contents are supplied into the container can be changed, and appropriate processing can be performed. For example, the direction in which contents are supplied can be changed in response to the processing.

[0047] Further, the processing device of the present application is a processing device in which the following processes are performed: a process of supplying contents from the first opening portion and discharging the contents from the second opening portion, and a process performed inside the container; and a process of supplying contents from the second opening portion and discharging the contents from the first opening portion, and a process performed inside the container.

[0048] With this configuration, the direction in which contents are supplied into the container can be changed, and appropriate processing can be performed. For example, the direction in which contents are supplied can be changed in response to the processing.

[0049] Further, the processing device of the present application is a processing device in which the following processes are performed: a process of supplying contents from the first opening portion and discharging the contents from the second opening portion, and a process performed inside the container; and a process of supplying contents from the second opening portion and discharging the contents from the first opening portion, and a process performed inside the container.

[0050] With this configuration, the direction in which contents are supplied into the container can be changed, and appropriate processing can be performed. For example, the direction in which contents are supplied can be changed in response to the processing.

[0051] Further, the processing device of the present application is a processing device in which the following processes are performed: a process of supplying contents from the first opening portion and discharging the contents from the second opening portion, and a process performed inside the container; and a process of supplying contents from the second opening portion and discharging the contents from the first opening portion, and a process performed inside the container.

[0052] This configuration allows for more efficient microwave irradiation compared to using single-mode microwaves.

[0053] Furthermore, the processing apparatus of the present invention is as follows: the processing apparatus further includes a device for irradiating microwaves into the container by the emission section.

[0054] This configuration allows for efficient microwave irradiation.

[0055] Invention Effects

[0056] The processing apparatus according to the present invention can efficiently perform microwave irradiation. Attached Figure Description

[0057] Figure 1 This is a perspective view showing an example of the processing apparatus in Embodiment 1 of the present invention. Figure 1 a)) and its Ib-Ib line profile ( Figure 1 (b)).

[0058] Figure 2 This is a perspective view of the filter of the processing device viewed from an angle above. Figure 2 (a) A three-dimensional view of the reflective component viewed from an oblique angle ( Figure 2 (b) A perspective view of the reflective element and the filter disposed on the reflective element, viewed from an oblique angle. Figure 2 (c) and a perspective view of the reflective element and the filter disposed on the reflective element from an oblique downward view. Figure 2 (d)).

[0059] Figure 3 This is a cross-sectional view used to illustrate a modified example of the processing device. Figure 3 (a)- Figure 3 (c)).

[0060] Figure 4 This is a perspective view showing an example of the processing apparatus in Embodiment 2 of the present invention. Figure 4 (a) and its cross-sectional view of IVb-IVb line ( Figure 4 (b)).

[0061] Figure 5 This is a perspective view showing an example of the processing apparatus in Embodiment 3 of the present invention. Figure 5 a)) and its Vb-Vb line profile ( Figure 5 (b)).

[0062] Figure 6 This is a cross-sectional view used to illustrate a first modified example of the processing apparatus. Figure 6 (a)- Figure 6 (c)).

[0063] Figure 7 is a sectional view to explain the second modification of the processing device (a) - (c). Figure 7 Figure 7

[0064] Figure 8 is a perspective view showing an example of the processing device in Embodiment 4 of the present application (a) and a sectional view thereof along lines VIIIb-VIIIb (b). Figure 8 Figure 8

[0065] Figure 9 is a perspective view showing an example of the column in Embodiment 5 of the present application (a) and a sectional view thereof along lines IXb-IXb (b). Figure 9 Figure 9

[0066] Figure 10 is a perspective view of the filter of the column viewed from an oblique upper side (a), a perspective view of the reflecting member viewed from an oblique upper side (b), a perspective view of the reflecting member and the filter in a state where the filter is disposed on the reflecting member (c), and a perspective view of the reflecting member and the filter in a state where the filter is disposed on the reflecting member viewed from an oblique lower side (d). Figure 10 Figure 10 Figure 10 Figure 10

[0067] Figure 11 is a perspective view of the column in Embodiment 5 of the present application installed in a state of a device to be processed (a) and a sectional view thereof along lines XIb-XIb (b). Figure 11 Figure 11

[0068] Figure 12 is a sectional view to explain the first modification of the column (a) - (c). Figure 12 Figure 12

[0069] Figure 13 is a sectional view to explain the second modification of the column (a) - (c). Figure 13 Figure 13

[0070] Figure 14 is a perspective view showing an example of the column in Embodiment 6 of the present application (a) and a sectional view thereof along lines XIVb-XIVb (b). Figure 14 Figure 14 DETAILED DESCRIPTION

[0071] ​​​​​​​​​​​​​​​​​​The following description refers to the accompanying drawings to illustrate embodiments of the processing apparatus, etc. Furthermore, since components marked with the same symbols in the embodiments perform the same actions, repeated descriptions are sometimes omitted.

[0072] (Implementation Form 1)

[0073] Figure 1 This is a perspective view showing an example of the processing apparatus in this embodiment. Figure 1 (a) and its cross-sectional view of the Ib-Ib line ( Figure 1 (b)). But Figure 1 (b) Omits the cross-section of the valve, etc.

[0074] Figure 2 This is a perspective view of the first filter of the processing device in this embodiment, viewed from an oblique angle. Figure 2 (a) A three-dimensional view of the first reflecting component viewed from an oblique angle ( Figure 2 (b) A perspective view of the first reflective member and the first filter disposed on the reflective member, viewed from an obliquely upward angle. Figure 2 (c) and a perspective view of the reflective element and the filter disposed on the reflective element from an oblique downward view. Figure 2 (d)).

[0075] The processing device 1 includes a container 101, an irradiation device 102, a first valve 103a, a first filter 105, and a first reflective member 106. The container 101 has a first opening 1011, a second opening 1012, and an irradiation opening 1013.

[0076] In this embodiment, processing apparatus 1 is described as an example of a processing apparatus used for solid-phase synthesis of peptides already bonded to a solid-phase resin. However, processing apparatus 1 may also be used for processing other than peptide solid-phase synthesis, as described later.

[0077] Here, we will first briefly describe an example of peptide solid-phase synthesis. However, solid-phase synthesis using the processing apparatus 1 of this embodiment is not limited to the solid-phase synthesis described below, and other solid-phase synthesis is also possible. For example, solid-phase synthesis using substances other than those described below is possible.

[0078] Solid-phase synthesis of peptides is one of the chemical methods for synthesizing peptides, also known as polypeptide solid-phase synthesis. In solid-phase synthesis, a solid resin is used, and the desired amino acid is bonded to the surface of the resin, which is suspended in a suitable solvent. The amino acid is then further bonded sequentially through a condensation reaction, elongating the peptide chain, thereby synthesizing a peptide bonded to the solid resin. Then, for example, the peptide bonded to the solid resin is cleaved from the resin to obtain the target peptide.

[0079] In solid-phase synthesis, the synthesis of a peptide is performed from the C-terminal side toward the N-terminal side. Therefore, first, an amino acid having a C-terminal amine group is bound to the surface of a solid-phase resin, and the synthesis is started. After the reaction of the solid-phase resin surface with the amino acid is completed, the solid-phase resin is washed with a solvent to remove the residual amino acid and the like. After the removal, if the protecting group of the amino acid bound to the solid-phase resin is removed (deprotected), the amine group of the next reaction site appears on the surface of the solid-phase resin again. Further, an N-terminally protected amino acid is added, and the C-terminal side of the N-terminally protected amino acid is bound to the N-terminal side of the amino acid appearing on the surface of the solid-phase resin through a condensation reaction. Then, the procedure is repeated while sequentially changing the amino acid to be used, and thus a peptide having a target sequence can be synthesized with high accuracy.

[0080] An example of a material and the like used in the above-described solid-phase synthesis will be described. As the solid-phase resin, a polymer such as polystyrene or polyamide can be used. However, the solid-phase resin can be a resin other than the above. For example, a resin in the form of particles (for example, beads) having a diameter of 10 μm to 1000 μm is used as the solid-phase resin. As a solvent in which the solid-phase resin is suspended, DMF (N,N-dimethylformamide) can be used, for example. However, another solvent can be used. The solid-phase resin is used as a carrier for solid-phase synthesis, for example, to bind and synthesize a peptide and the like.

[0081] Further, the solid-phase resin can be bound to the C-terminal amino acid indirectly through a linker molecule and the like, instead of being directly bound to the C-terminal amino acid. As the linker molecule, 4-hydroxymethylphenoxyacetic acid (HMP) or a benzhydrylamine derivative can be used, for example.

[0082] As the protecting group of the amino acid, Boc (tert-butyloxycarbonyl) or Fmoc (9-fluorenylmethyloxycarbonyl) and the like can be used, for example.

[0083] The removal of the protecting group, that is, deprotection, can be performed by treatment with a base such as piperidine used as a deprotection agent, for example.

[0084] The process of sequentially binding amino acids to the solid-phase resin is performed in a solution containing the amino acid. For example, the process of sequentially binding amino acids to the solid-phase resin is performed in a solution having an amino acid, an activating agent, a racemization inhibitor, and the like. The activating agent can be used to promote the binding of the deprotected amino acid that has been bound to the solid-phase resin (including an amino acid bound to the terminal of a peptide bound to the solid-phase resin) to an N-terminally protected amino acid in the solution. The activating agent is also referred to as a condensing agent. Further, the racemization inhibitor can be used to inhibit the occurrence of racemization and prevent a decrease in the reaction and the like due to racemization. As the activating agent, DIPC1 (N,N'-diisopropylcarbodiimide) or HBTU (N,N,N',N'-tetramethyl-O-(benzotriazol-1-yl)uronium hexafluorophosphate) and the like can be used, for example. Further, as the racemization inhibitor, Oxyma (ethyl(hydroxyimino)cyanoacetate) and the like can be used, for example.

[0085] Among the various processes performed in solid phase synthesis, the processes can be promoted and performed at high speed by irradiating microwaves. For example, Patent Literature 1 and the like disclose that when a protecting group is removed from the N-terminus of an amino acid bound to a solid phase resin, that is, when deprotection is performed, the deprotection step can be promoted and the process time can be shortened by irradiating microwaves. Further, the so-called amino acid bound to a solid phase resin can be considered to also include an amino acid bound to the terminal of a peptide bound to a solid phase resin. The same applies hereinafter. Also, when a deprotected amino acid bound to a solid phase resin is condensed with an amino acid in a solution, the process time for condensation can be shortened by irradiating microwaves.

[0086] The processing device 1 of the present embodiment is a device for performing one or more processes among the processes constituting such solid phase synthesis. For example, two or more processes performed using the processing device 1 of the present embodiment can be consecutive two or more processes or discontinuous two or more processes. The one or more processes performed using the processing device 1 of the present embodiment can be any of the processes of solid phase synthesis. The one or more processes performed using the processing device 1 of the present embodiment preferably include one or more processes including, for example, a process of irradiating microwaves to a solid phase resin suspension. For example, the one or more processes can include a process of deprotecting an N-terminally protected amino acid bound to a solid phase resin. Also, the one or more processes can include a process of performing a condensation reaction on a deprotected amino acid bound to a solid phase resin, thereby condensing the amino acid. Also, the one or more processes performed using the processing device 1 of the present embodiment preferably include one or more processes including a process of separating a solid phase resin by filtration. The solid phase resin here is, for example, a solid phase resin to which one or more amino acids are bound. For example, the process can be a process of filtering a solid phase resin from a solid phase resin suspension after the amino acid has been bound to the solid phase resin, or a process of separating a solid phase resin by filtration from a solid phase resin suspension after a deprotection process has been performed on a solid phase resin to which an N-terminally protected amino acid is bound. Also, the process can be a process of filtering a solid phase resin after the solid phase resin has been washed with a washing liquid such as a solvent. Also, the one or more processes performed using the processing device 1 of the present embodiment can be a combination of two or more of the above-described processes. For example, the one or more processes performed using the processing device 1 of the present embodiment can be a repetition of a peptide chain elongation process composed of the following processes: a process of deprotecting a solid phase resin to which an N-terminally protected amino acid is bound, a process of filtering and washing a solid phase resin that has been deprotected, a process of condensing a deprotected amino acid of the solid phase resin after washing with an N-terminally protected amino acid, and a process of filtering and washing the solid phase resin.

[0087] The container 101 is a container in which one or more solid-phase synthesis processes are performed. For example, a substance used for solid-phase synthesis, an intermediate product, a solvent, and the like are supplied and held in the container 101. The substance used for solid-phase synthesis is, for example, a solid-phase resin, an amino acid, a deprotection agent, an activating agent, a racemization inhibitor, and the like. The solid-phase resin is, for example, a solid-phase resin to which one or more amino acids have been previously bound, a solid-phase resin to which a linker molecule and one or more amino acids have been bound, or a solid-phase resin to which no amino acid has been bound. Also, the solid-phase resin can be a solid-phase resin to which only a linker molecule has been bound. For example, the substance or the intermediate product is held in the container 101 together with a suitable solvent. Also, for example, a liquid used for washing and the like, a gas used for stirring and the like, and the like can be supplied from the outside into the container 101. Further, the supply of the contents of the container 101 and the discharge of the contents can be performed continuously, and the process can be performed continuously while the contents flow in the container 101.

[0088] The container 101 is made of a material having microwave reflectivity. The material having microwave reflectivity is, for example, an electric conductor. The electric conductor having microwave reflectivity is, for example, a metal such as stainless steel. The container 101 is preferably made of a material having excellent corrosion resistance. For example, the container 101 is preferably made of stainless steel. The thickness of the outer wall and the like of the container 101 is not limited. The inner wall of the container 101 can be coated with a material such as polytetrafluoroethylene (PTFE) or glass, which has high microwave permeability and excellent corrosion resistance and the like. For example, the container 101 can be a double-structured container in which the inner wall is made of the material having high microwave permeability and excellent corrosion resistance and the like, and the outer wall is made of a microwave-reflective material such as stainless steel.

[0089] The container 101 is preferably structured such that microwave does not leak to the outside when microwave is irradiated into the inside. For example, the container 101 is preferably structured such that the inside of the container 101 can be sealed when microwave is irradiated.

[0090] The container 101 has a first end portion 1015a and a second end portion 1015b. The end portion is, for example, a portion or a region of the tip of the container 101. The end portion can be a point, a line, or a surface. The first end portion 1015a and the second end portion 1015b are, for example, opposite to each other. The first end portion 1015a and the second end portion 1015b are, for example, portions of both ends in the length direction of the container 101. Here, a case where the container 101 is a vertical container is described. The vertical container means, for example, a container in which the length direction is substantially vertical. The container 101 has a capsule shape in which the upper and lower portions are hemispherical and the intermediate portion is cylindrical. Therefore, in a cross-sectional shape obtained by cutting the container 101 in the vertical direction, the end portions in the length direction are, for example, semicircular. Figure 1(b) is shown to have a semicircular shape. Here, a case where the first end portion 1015a is the lower end portion of the container 101 and the second end portion 1015b is the upper end portion is taken as an example to be described. The lower end portion of the container 101 is the end portion of the lower side of the container 101. The lower end portion can also be, for example, a portion of the lower surface or the bottom of the container 101. Also, the upper end portion of the container 101 is the end portion of the upper side of the container 101. The upper end portion can also be, for example, a portion of the upper surface of the container 101. Furthermore, a case where a portion on the first end portion 1015a side (e.g., the lower end portion) of the container 101 has a shape in which the size continuously or stepwise decreases as it approaches the first end portion 1015a is described here. Also, a case where a portion on the second end portion 1015b side (e.g., the upper end portion) of the container 101 has a shape in which the size continuously or stepwise decreases as it approaches the second end portion 1015b is described. Furthermore, the size of the portion on the first end portion 1015a side of the container 101 and the size of the portion on the second end portion 1015b side of the container 101 can be, for example, the width of the container 101, the size (e.g., the cross-sectional area, etc.) in a direction perpendicular to the length direction of the container 101, the length in a direction perpendicular to the length direction of the container 101, or the width of the container 101.

[0091] Furthermore, the container 101 can also be any shape, such as a shape other than the above. For example, the shape of the container 101 can be a shape other than a capsule shape, such as a cylindrical shape, a polygonal column shape, a conical shape, a combination of the above shapes, or the like. Also, the shape on the upper end portion side of the container 101 can not be a semispherical shape, such as a flat surface. The container 101 preferably has a shape that is a shape sought by rotating a shape (e.g., a circular shape, an elliptical shape, a rounded square shape, etc.) about an axis of extension in a vertical direction or a horizontal direction as a center of rotation, but can not have a shape that is a rotated shape. Also, the container 101 can be a shape that is a face-symmetrical shape with respect to a plane perpendicular to the length direction, or can not be a face-symmetrical shape. The portion on the first end portion 1015a side (e.g., the lower end portion) of the container 101 is preferably, for example, a shape in which the size continuously or stepwise decreases as it approaches the first end portion 1015a. Also, the portion on the second end portion 1015b side (e.g., the upper end portion) of the container 101 is preferably, for example, a shape in which the size continuously or stepwise decreases as it approaches the second end portion 1015b.

[0092] The dimensions of the container 101 of the present embodiment are a diameter of 1 m and a height of 2 m, but these dimensions are merely examples, and the container 101 can be larger or smaller, and the size of the container 101 is not limited. Also, the ratio of the height, the width, and the depth of the container 101, etc. is not limited.

[0093] The shape, size, etc. of the container 101 are determined, for example, in accordance with the distribution of microwaves applied to the container 101. For example, the shape and size of the container 101 are preferably set so that the mode of microwaves within the container 101 becomes multimode. By multimode of microwaves, for example, is meant a mode in which standing waves of microwaves do not occur within the container 101.

[0094] Although not shown in the drawings, a warm water jacket, a cold water jacket, a heater, etc. for adjusting the temperature of the container 101 can be provided on the outer periphery of the container 101.

[0095] The lower portion of the container 101 is provided with a first opening portion 1011. The lower portion of the container 101 herein is, for example, the side of the first end portion 1015a of the container 101, that is, the lower end portion side. The first opening portion 1011 is provided, for example, at the first end portion 1015a of the container 101. The first opening portion 1011 is an opening portion, that is, a discharge port, for discharging the contents within the container 101. The contents discharged herein can not necessarily be all of the contents. By the contents within the container 101, for example, are meant the substances, solvents, etc. used in the solid-phase synthesis described above, the liquid for cleaning (for example, a solvent), etc. The liquid contents are a concept including solutions and suspensions, etc. The contents discharged from the first opening portion 1011, which is a discharge port, are, for example, the portion of the contents held within the container 101 before discharge that is filtered by the first filter 105 described below, and the portion of the contents from which the solid-phase resin separated by filtration is removed. An example in which one first opening portion 1011 is provided in the container 101 is shown herein, but a plurality of first opening portions 1011 can be provided. The first opening portion 1011 is preferably provided at the lowermost portion of the container 101 in such a manner that the contents within the container 101 are naturally discharged. The lowermost portion of the container 101 is usually the lower end portion 1015a of the container 101. The size and shape of the first opening portion 1011 are not limited.

[0096] Further, the size of the first opening portion 1011 is preferably smaller than the size of the portion of the container 101 away from the first end portion 1015a (for example, the portion near the center in the length direction of the container 101, or the portion provided with the first filter 105 or the first reflecting member 106).

[0097] The container 101 is provided with a second opening portion 1012 for supplying a substance, a solvent, a cleaning liquid, a gas, or the like used for solid-phase synthesis into the container 101. The size and the like of the second opening portion 1012 are not limited. Here, an example in which the second opening portion 1012 is provided at the upper portion of the container 101 is described, but the second opening portion 1012 can be provided at a position other than the upper portion (for example, the side portion or the lower portion of the container 101, or the like). The second opening portion 1012 is provided, for example, at a position higher than the first opening portion 1011, that is, at the side of the second end portion 1015b. However, the second opening portion 1012, which supplies the solid-phase resin at a position higher than the first filter 105 described later, is preferably provided at a position higher than the first filter 105 described later. Further, the upper portion of the container 101 is, for example, a portion on the side of the second end portion 1015b, that is, on the side of the upper end portion, of the container 101. The second opening portion 1012 is preferably blocked by a lid or a plug, or the like, not shown in the drawing, when not being supplied. Here, an example in which the second opening portion 1012 is one is described, but the second opening portion 1012 can be a plurality of openings. The second opening portion 1012 can be connected to a pipe (not shown) or the like for sending the content supplied into the container 101 to the second opening portion 1012. Further, the second opening portion 1012 can be provided with one or more nozzles (not shown) or the like extending into the container 101 for supplying the content or the like into the container 101. The nozzles can be detachable members. Here, an example in which the second opening portion 1012 is closed by the lid 1012a that can be opened and closed is described.

[0098] Further, a stirring device (not shown) for stirring the content in the container 101 can be provided. The stirring device can be realized by, for example, a stirring blade, a rotation shaft provided at the center of rotation of the stirring blade, and a motor or the like that rotates the rotation shaft. The number and the shape of the stirring blade are not limited. Further, the direction in which the rotation shaft extends can be the vertical direction, the horizontal direction, or a direction other than the above. Further, the stirring device can be a device that stirs the content by blowing bubbles into the liquid content (that is, by froth stirring). The stirring device can be, for example, a combination of an opening portion or a nozzle provided at the lower portion or the side portion of the content or the like that blows an inert gas such as nitrogen, and a gas supply device such as a cylinder that is connected to the opening portion or the nozzle and supplies the gas thereto. Further, a gas that does not affect the solid-phase synthesis or affects the solid-phase synthesis little can be used instead of the inert gas.

[0099] Further, the container 101 can be provided with a temperature sensor or the like inside or outside to measure the temperature inside the container 101 (not shown). The temperature inside the container 101 can be feedback controlled using the value measured by the temperature measuring device. Further, a sensor other than the temperature sensor can be provided inside the container 101. Further, the container 101 can be provided with an observation window (not shown) to observe the inside of the container 101. The observation window is, for example, made of a material having high microwave reflectivity and has a size that does not leak microwaves from the inside of the container 101, and can be implemented by a cylindrical member blocked with glass or the like. However, the configuration of the observation window is not limited. Further, the container 101 can be a container in which the internal pressure can be changed. For example, the container 101 can be a container in which the internal pressure can be reduced or increased. The container 101 can be connected to a device (not shown) such as a pump that changes the pressure inside the container 101, for example.

[0100] The irradiation device 102 is a device that irradiates microwaves into the container 101. The irradiation device 102 is, for example, attached to the container 101 in such a manner that microwaves can be irradiated into the container 101. The irradiation device 102 has three sets of microwave oscillators 1021 and waveguides 1022, and irradiates microwaves into the container 101 at three positions. The three waveguides 1022 of the irradiation device 102 are attached to the container 101 in such a manner that the opening portions at the respective end portions communicate with the irradiation opening portions 1013 provided at three different positions on the container 101. The irradiation opening portions 1013 are opened in such a manner that the inside of the container 101 communicates with the outside. Further, the irradiation opening portions 1013 are, for example, opening portions used to irradiate microwaves into the inside of the container 101. The inside of the container 101 can be the inside of the container 101 or the inside of the container 101. Further, the irradiation opening portions 1013 are, for example, a guide inlet to guide microwaves into the container 101 or a portion that causes microwaves irradiated into the container 101 to be emitted. The end portion of each waveguide 1022 connected to the irradiation opening portion 1013 of the container 101 is, for example, the end portion of each waveguide 1022 on the side opposite to the end portion connected to the microwave oscillator 1021. The end portion of each waveguide 1022 connected to the irradiation opening portion 1013 of the container 101 is the end portion from which the irradiation device 102 emits microwaves, and the position to which the end portion is connected is, for example, the position from which the irradiation device 102 emits microwaves. Here, the end portions of the three waveguides 1022 are connected to the same positions as the height positions on the upper portion of the container 101, and the end portions of the three waveguides 1022 are arranged at equal intervals around the assumed central axis of the container 101 in the longitudinal direction.

[0101] Each microwave oscillator 1021 generates microwaves. The microwaves generated by each microwave oscillator 1021 are transmitted to a waveguide 1022 connected to each microwave oscillator 1021, and are emitted into the container 101 through the irradiation opening portion 1013 from the end portion of the waveguide 1022 connected to the container 101, respectively. Thus, the microwaves generated by each microwave oscillator 1021 become the microwaves emitted by the irradiation device 102. The frequency, intensity, and the like of the microwaves emitted by each microwave oscillator 1021 are not limited. The frequency of the microwaves emitted by each microwave oscillator 1021 can be, for example, 915 MHz, 2.45 GHz, 5.8 GHz, or a frequency in the range of 300 MHz to 300 GHz. The microwave oscillator 1021 is, for example, a magnetron, a klystron, a gyrotron, or a semiconductor oscillator.

[0102] The waveguide 1022 is used as a transmission portion that transmits microwaves. The waveguide 1022 is generally a waveguide having a shape that matches the frequency of the microwaves generated by the microwave oscillator 1021. Furthermore, the irradiation opening portion 1013 connected to the end portion of the waveguide 1022 can be blocked by a material such as a fluorinated polymer such as PTFE, glass, rubber, and nylon, which has high microwave permeability. For example, it can be blocked by a plate or the like made of the material. The position at which the irradiation device 102 emits microwaves into the container 101 can be, for example, the center 1023 of the opening portion of the end portion of the waveguide 1022 connected to the container 101. The position at which microwaves are emitted can be the center of the irradiation opening portion 1013. Furthermore, the waveguide 1022 can be attached to the container 101 such that the end portion on the container 101 side protrudes into the container 101. For example, the end portion of the waveguide 1022 on the container 101 side can also protrude into the container 101 through the irradiation opening portion 1013. Also, the waveguide 1022 can be blocked by a material such as a fluorinated polymer such as PTFE, glass, rubber, and nylon, which has high microwave permeability. For example, it can be blocked by a plate or the like made of the material. The position at which the waveguide 1022 is blocked is generally the portion on the container 101 side (for example, the end portion on the container 101 side), but can be a portion on the microwave oscillator 1021 side or another position, and the position is not limited. Furthermore, the irradiation opening portion 1013 can also be blocked by a material having high microwave permeability, and as a result, the waveguide 1022 can also be blocked.

[0103] Furthermore, the irradiation device 102 can be a member other than the above, as long as it is a member that can irradiate microwaves into the container 101. For example, the irradiation device 102 is not limited to irradiating microwaves into the container 101 from three positions, but can irradiate microwaves into the container 101 from one or more than two positions. For example, the irradiation device 102 can have one set or more than two sets of microwave oscillators 1021 and waveguides 1022 connected to the irradiation opening portions 1013 provided to the container 101, and can emit the microwaves generated by each microwave oscillator 1021 into the container 101 through the waveguide 1022.

[0104] Further, instead of connecting one waveguide 1022 to one microwave oscillator 1021, a plurality of waveguides 1022 having a structure branched into two or more can be connected to one microwave oscillator 1021, and the ends of the branched waveguides 1022 can be connected to different irradiation opening portions 1013 provided in the container 101. In this case, the microwaves generated by one microwave oscillator 1021 are branched by the waveguides 1022, and are emitted into the container 101 from a plurality of positions in the container 101.

[0105] The position at which the irradiation device 102 emits microwaves is not limited. The position at which the ends of one or a plurality of waveguides 1022 are connected to the container 101, i.e., the position at which the irradiation device 102 is connected to the container 101, can be the upper portion, or can not be the upper portion. For example, the ends of the waveguides 1022 can be connected to the side portion of the container 101, or the like. The connection position is determined, for example, in accordance with the shape of the container 101, the height of the contents held in the container 101, the wavelength of the microwaves emitted by the irradiation device 102, or the like. The upper portion of the container 101, for example, is a region including the upper end portion of the container 101. The following example is described in the present embodiment: the ends of the waveguides 1022 are connected to the upper portion of the container 101, and the irradiation device 102 emits microwaves into the container 101 from a position higher than the first opening portion 1011 of the container 101, for example.

[0106] Further, the irradiation device 102 can further include an antenna (not shown) connected to the ends of the waveguides 1022 connected to the container 101 or the irradiation opening portions 1013 to which the ends are connected, for example. The antenna is provided in the container 101 to emit microwaves into the container 101. In this case, the antenna becomes a portion of the irradiation device 102 that emits microwaves, and the position at which the irradiation device 102 emits microwaves can be the position at which the antenna emits microwaves, for example.

[0107] Further, in the irradiation device 102 described above, a transmission path that transmits the microwaves generated by the microwave oscillator 1021 can be a coaxial cable or another transmission portion instead of the waveguide 1022. For example, the other end of a coaxial cable having one end connected to the microwave oscillator 1021 can be inserted into the irradiation opening portion 1013 provided in the container 101, and microwaves can be emitted into the container 101 through the coaxial cable. Further, in the case where the microwaves generated by the microwave oscillator 1021 are emitted into the container 101 without using a transmission portion such as the waveguide 1022, the transmission portion can be omitted.

[0108] Further, the irradiation device 102 can further have an antenna (not shown) connected to the end portion of the container 101 (or the irradiation opening portion 1013 connected to the end portion) via a transmission portion such as a coaxial cable. The antenna is provided in the container 101 to radiate microwaves into the container 101. In this case, the antenna becomes a portion of the irradiation device 102 from which microwaves are radiated, and the position from which the irradiation device 102 radiates microwaves can be, for example, the position from which the antenna radiates microwaves.

[0109] Further, the irradiation device 102 can be a member that radiates microwaves of different frequencies into the container 101. For example, the irradiation device 102 can have a plurality of microwave oscillators 1021 that generate microwaves of different frequencies, and radiate microwaves of different frequencies from each microwave oscillator 1021. The irradiation device 102 can radiate microwaves of different frequencies into the container 101 at the same time, or can switch the microwaves of different frequencies and radiate them at different times. Further, the irradiation device 102 can have one or more microwave oscillators 1021 that can change the frequency of the microwaves radiated therefrom, and can cause the microwaves radiated into the container 101 to be microwaves of different frequencies by appropriately changing the frequency of the microwaves radiated from each microwave oscillator 1021.

[0110] The first filter 105 is a member that separates the solid-phase resin used for solid-phase synthesis from the contents in the container 101. The solid-phase resin used for solid-phase synthesis is, for example, a solid that is the target of separation from the contents. The first filter 105 is a member that separates, for example, by filtration, a solid having a size (e.g., particle diameter, etc.) smaller than the solid-phase resin (e.g., a liquid, a solid having a size smaller than the solid-phase resin, etc.) and a solid having a size equal to or greater than the solid-phase resin. The first filter 105 is, for example, formed of a microwave-transmissive material. The microwave-transmissive material is, for example, a material having high microwave transmissivity. The material having high microwave transmissivity is, for example, a material having a small relative dielectric loss. Further, the microwave-transmissive material is preferably formed of a chemically inert material having excellent corrosion resistance. For example, the first filter 105 is formed of a fluorinated polymer such as PTFE, polypropylene, quartz, glass, nylon, rubber, or the like.

[0111] The first filter 105 has, for example, a plurality of pores for separating the solid phase resin used for the solid phase synthesis from the contents in the container 101. The pores are, for example, pores that are communicated from the upper surface 1051 to the back surface. The upper surface 1051 of the first filter 105 is, here, the surface on the side of the second end 1015b of the first filter 105. The first filter 105 is, for example, constituted of a porous material. However, the first filter 105 can also be a mesh or the like. The contents in the container 101 are, for example, a liquid (for example, a suspension) containing the solid phase resin used for the solid phase synthesis as described above. The liquid is, for example, a liquid for dissolving or suspending the above-described activating agent, the amino acid or the like used for the solid phase synthesis. Also, the liquid containing the solid phase resin can be a suspension or the like of the solid phase resin and a liquid such as a solvent for washing the solid phase resin. The plurality of pores of the first filter 105 are, for example, pores of a size that cannot pass the solid phase resin contained in the contents, and are pores of a size that can pass the contents other than the solid phase resin. Further, the size of the plurality of pores can be, for example, a size that can pass all of the contents other than the solid phase resin of the contents, or a size that can pass a part of the contents other than the solid phase resin. By the contents other than the solid phase resin, for example, there are the solvent, the activating agent, the deprotecting agent or the like used for the solid phase synthesis in a state of being dissolved in the solvent, the amino acid or the like in a state of being dissolved or suspended in the solvent, and the like, and they are smaller in size than the solid phase resin. The plurality of pores of the first filter 105 can be, for example, pores that are smaller in size than the solid phase resin contained in the contents and larger in size than the contents other than the solid phase resin. For example, the plurality of pores of the first filter 105 are smaller in size than the particle diameter of the solid phase resin contained in the contents. By this, only the solid phase resin in the contents in the container 101 is separated by filtration and remains on the upper surface 1051 of the first filter 105, and the activating agent or the deprotecting agent or the like dissolved in the solvent, or the amino acid or the like dissolved or suspended in the solvent, passes through the pores of the first filter 105 along with the solvent. For example, in a case where a particulate solid phase resin having a diameter of 50 μm to 150 μm is used as the solid phase resin, the diameter of the pores of the first filter 105 is preferably smaller than the value, for example, smaller than 50 μm or the like.

[0112] Further, the solid-phase resin separated by the first filter 105 can be a solid-phase resin monomer, or a solid-phase resin to which a linker molecule, one or more amino acids, or a peptide, or the like has been bound. For example, the first filter 105 can have a plurality of pores for separating a solid-phase resin to which an amino acid or a peptide has been bound from the contents in the container 101. The solid-phase resin separated from the contents by the first filter 105 can be referred to as a resin-containing solid that contains a solid of a solid-phase resin, regardless of whether or not a linker molecule, one or more amino acids, or a peptide, or the like has been bound. For example, the first filter 105 can be a member that separates a resin-containing solid from the contents in the container 101. The same applies to the first reflecting member 106 described later. However, the size of the solid-phase resin that can be used is not limited to this size.

[0113] The first filter 105 is disposed so as to divide the container 101 between the first opening portion 1011 of the container 101 and the position at which the irradiation device 102 emits microwaves. The position at which the irradiation device 102 emits microwaves is hereinafter referred to as the emission position. The emission position can be the center 1023 of the opening portion of the waveguide 1022 described above. Further, the emission position can be, for example, the position of the irradiation opening portion 1013. The first filter 105 is disposed so as to divide the container 101 into two regions vertically between the first opening portion 1011 and the emission position. By the first filter 105 being disposed between the first opening portion 1011 and the emission position, for example, the first filter 105 is disposed so that the position of the first filter 105 in the longitudinal direction of the container 101 is between the position of the first opening portion 1011 and the emission position. By the first filter 105 being disposed so as to divide the container 101, for example, the first filter 105 is disposed so that the contents in the container 101 do not pass through the first filter 105 and do not move. In the present embodiment, an example is shown in which the first filter 105 is disposed so that no gap is present between the outer periphery of the first filter 105 and the side surface of the container 101. In the case where the first filter 105 has a substantially planar surface, for example, the first filter 105 is preferably disposed so that the surface is perpendicular to the longitudinal direction of the container 101. By the first filter 105 being disposed so as to divide the container 101 between the first opening portion 1011 and the emission position of the irradiation device 102, for example, the first filter 105 is disposed between the first opening portion 1011 and the emission position of the irradiation device 102, and at the same time, the first filter 105 is disposed so that the contents in the container 101 do not pass through the first filter 105 and do not move between the region on the side of the irradiation device 102 with respect to the first filter 105 and the region on the side of the first opening portion 1011 with respect to the first filter 105.

[0114] In the present embodiment, as an example, a sheet-shaped first filter 105 made of a porous material made of PTFE is used. The first filter 105 has a substantially flat upper surface 1051, and is arranged in the container 101 so that the upper surface 1051 is horizontal. In the present embodiment, the first filter 105 is arranged in the container 101 in the above-described manner, and thus the container 101 is divided into upper and lower portions by the first filter 105. However, the upper surface 1051 of the first filter 105 can not be substantially flat, and can have, for example, a concave-convex shape. Also, the first filter 105 can be arranged so that the upper surface 1051 is inclined with respect to the horizontal.

[0115] Further, although the first filter 105 is described herein as being sheet-shaped, the first filter 105 can be a first filter having a shape other than a sheet shape, such as a flat plate shape. Also, the thickness, strength, and the like of the first filter 105 are not limited. The first filter 105 preferably has a substantially uniform thickness, but can have a non-uniform thickness.

[0116] Further, the first filter 105 is preferably arranged at a position at a height of 1 / 4 or less of the height of the container 101 in order to sufficiently secure a region in which the solid-phase growth process is performed, but the first filter 105 can be arranged at a position higher than 1 / 4 of the height of the container 101.

[0117] The first reflecting member 106 is arranged so as to divide the container 101 between the first filter 105 and the first opening portion 1011 in the container 101. The first reflecting member 106 is arranged at a position away from the first opening portion 1011 in the length direction (in this case, the height direction) of the container 101. In this case, as described above, the first reflecting member 106 is arranged so that the upper surface 1061 is horizontal in the container 101. Figure 2 (c) and Figure 2 (d), the first filter 105 is arranged so as to overlap the first reflecting member 106, that is, on the side of the second end portion 1015b of the first reflecting member 106. By "arranged so as to overlap the first reflecting member 106", it is meant, for example, that the first filter 105 is arranged on the side of the second end portion 1015b of the first reflecting member 106, that is, on the upper surface 1061. In this case, the first reflecting member 106 is a flat plate-shaped member, and the upper surface 1061 is substantially flat, and the first filter 105 is arranged so as to overlap the upper surface 1061 in the container 101. Further, the upper surface 1061 of the first reflecting member 106 can not be substantially flat. Also, the first reflecting member 106 can not be arranged so that the upper surface 1061 is horizontal. Also, the first reflecting member 106 can not be a flat plate-shaped member. The upper surface 1061 of the first reflecting member 106 is preferably a shape in which the first filter 105 can be stably placed. Further, the configuration in which the first filter 105 overlaps the first reflecting member 106 and the like described herein can also be applied to a configuration in which another filter or the like is arranged so as to overlap a reflecting member or the like.

[0118] The material of the first reflecting member 106 is a material having microwave reflectivity, i.e., stainless steel. The first reflecting member 106 is a plurality of holes 106a having a planar shape of a circular shape, which are sized to allow passage of the contents in the container 101 through the at least first filter 105. The holes 106a are, for example, holes that communicate from the upper surface 1061 to the back surface 1062. The diameter of each hole 106a is sized to allow passage of the contents in the container 101 through the at least first filter 105, and is sized to the microwave emitted by the irradiation device 102. By the sized to allow passage of the contents in the container 101 through the at least first filter 105, it is meant, for example, a size that allows passage of the contents in the container 101 through the first filter 105, and can be, for example, a size that allows passage of the portion of the contents in the container 101 other than the solid-phase resin separated by the first filter 105, or a size that allows passage of the contents including the solid-phase resin. As long as the diameter of the hole provided on the plate composed of a material having microwave reflectivity, or the width of the widest portion of the opening of the mesh composed of a material having microwave reflectivity, is smaller than the half wavelength of the microwave emitted by the irradiation device 102, the microwave is reflected in the plate or mesh, and as long as the diameter of the plurality of holes 106a provided in the first reflecting member 106 is, for example, smaller than the half wavelength of the microwave emitted by the irradiation device 102, and larger than the size that allows passage of the contents through the first filter 105. Further, the size of the hole 106a of the first reflecting member 106 is preferably, for example, a size that is smaller than the half wavelength of the microwave emitted by the irradiation device 102, and does not hinder passage of the contents through the first filter 105. The number or arrangement pattern of the plurality of holes 106a, etc. are not limited. In the present embodiment, the first reflecting member 106 is, for example, a punched metal sheet made of stainless steel having a plurality of holes 106a. In general solid-phase synthesis, the solid-phase resin is the largest in size among the contents in the container 101, and as long as the solid-phase resin can pass through the first reflecting member 106, the contents other than the solid-phase resin can also pass through the first reflecting member 106. Further, Figure 2 (b)、 Figure 2 (d) and the like are diagrams for illustration, and the size of the plurality of holes provided on the first reflecting member 106, the arrangement of the holes 106a, or the number of holes 106a, etc. are for illustration, and are not necessarily the same as the actual first reflecting member 106.

[0119] Further, the reflection of the microwave by the first reflecting member 106 here can be the entire reflection of the microwave irradiated to the first reflecting member 106, but can also not be the entire reflection. For example, a portion of the irradiated microwave can pass through the first reflecting member 106 and the remainder can be reflected, or a portion of the irradiated microwave can be absorbed by the first reflecting member 106.

[0120] Further, the first reflection member 106 can be made of any material as long as it reflects microwaves emitted from the irradiation device 102, and can have any shape and size. For example, the first reflection member 106 can be made of a material other than stainless steel that reflects microwaves, such as a metal other than stainless steel. However, the material of the first reflection member 106 is preferably a material that is excellent in corrosion resistance and stable in chemical properties. Further, the material of the first reflection member 106 is a metal, and the surface thereof can be coated with a material that is corrosion-resistant and transmits microwaves, such as PTFE, whereby the corrosion resistance can be improved without impairing the microwave reflection. Also, the planar shape of the plurality of holes provided in the first reflection member 106 can be a shape other than the above-described circular shape (for example, a polygonal shape) as long as it has a size that reflects microwaves. The first reflection member 106 can be configured, for example, to be made of a material that reflects microwaves and have a plate shape that has a plurality of holes that do not transmit microwaves emitted from the irradiation device 102.

[0121] Also, the first reflection member 106 can have any shape and size as long as it has a shape that allows the contents in the container 101 to pass through the at least first filter 105 and reflects microwaves, and can not be a flat plate-shaped member having a plurality of holes as described above. For example, the first reflection member 106 can be a mesh made of a material that reflects microwaves and have a plurality of opening portions that are holes having a size that does not allow microwaves to pass therethrough. Also, the first reflection member 106 can be a member other than the above-described member, such as a porous metal filter having holes through which a solid-phase resin can pass, as long as it allows the contents to pass through the at least first filter 105 and reflects microwaves. Further, when the first filter 105 is supported by the first reflection member 106 from below, the material of the first reflection member 106 is preferably a material that is high in strength, such as a metal.

[0122] The first reflection member 106 is disposed in a manner that divides the container 101 into upper and lower portions. By the first reflection member 106 being disposed in a manner that divides the container 101, for example, the first reflection member 106 is disposed in the container 101 in a manner that microwaves emitted from above the first reflection member 106 into the container 101 do not pass to a portion lower than the first reflection member 106. Also, by the container 101 being divided into upper and lower portions, for example, the container 101 is divided into regions in the upper and lower directions. For example, in the present embodiment, the first reflection member 106 is disposed in the container 101 in a manner that the upper surface 1061 of the first reflection member 106 is horizontal, whereby the first reflection member 106 divides the container 101 into the upper and lower directions, and divides the container 101 into an upper region 101a and a lower region 101b by the first reflection member 106.

[0123] In the processing device 1 of the present embodiment, microwaves are irradiated from the irradiation device 102 into the container 101 made of a microwave-reflective material, whereby the microwaves are confined in the container 101, and the microwaves can be efficiently irradiated to the contents. Also, the microwaves in the container 101 are made to be multimode, and the microwaves are not concentrated in one place, compared to the case of single mode, and the microwaves can be more uniformly irradiated to the contents. Therefore, even if the container 101 is made large, the microwaves can be efficiently and uniformly irradiated to the contents. Thus, in the present embodiment, the container 101 can be made large, and the amount of the peptides and the like processed by solid-phase synthesis can be increased.

[0124] Also, the processing device 1 of the present embodiment is provided with the first filter 105, whereby the solid-phase resin can be separated from the contents supplied above the first filter 105 and left in the container 101. Thus, the solid-phase resin can be washed with a washing solvent supplied in the container 101 in which the solid-phase resin is separated, or other materials or solvents, solutions, and the like can be supplied in the container 101 in which the solid-phase resin is separated, and other processes constituting solid-phase synthesis can be performed without temporarily taking out the solid-phase resin.

[0125] In the large container in which a large amount of contents can be processed, the size of the discharge port is usually small relative to the size of the entire container 101. Therefore, in the processing device 1 of the present embodiment, in order to easily discharge the contents at the time of discharge, the container 101 is often shaped such that the portion of the container 101 on the first end portion 1015a side, that is, the lower portion, becomes narrower toward the first opening portion 1011, which is used as the discharge port on the second end portion 1015b side, that is, the upper side. At this time, if the first filter 105 used to separate the solid-phase resin is disposed in a manner of dividing the container 101 into upper and lower portions near the first opening portion 1011 as described above, the upper surface area of the first filter 105 becomes smaller than when the first filter 105 is disposed at a position away from the first opening portion 1011. If the area of the upper surface 1051 of the first filter 1055 becomes smaller in this way, the speed of filtering the contents at the time of discharging the contents decreases, and the first filter 105 is easily clogged with the solid-phase resin filtered and left on the first filter 105, so that the filtering speed becomes slower or the filtering stops. Therefore, in the present embodiment, in order to increase the upper surface area of the first filter 105, the first filter 105 is preferably disposed at a position away from the first opening portion 1011 in the height direction in a manner of dividing the container 101 into upper and lower portions as described above.

[0126] In the processing apparatus 1, if the contents including the solid-phase resin or the solvent or the like used for the solid-phase synthesis are supplied to the area above the first filter 105, the first filter 105 is provided in the area below the first filter 105, that is, the area on the first end portion 1015a side in the container 101, and thus the contents held in the area above the first filter 105 are supplied through the first filter 105 from which the solid-phase resin is removed. Further, the area here can be a space.

[0127] However, in the processing apparatus 1 of the embodiment, in the processing apparatus in which the first filter 105 through which the solid-phase resin does not pass has the microwave permeability, when the first reflecting member 106 is not provided, in order to promote the processing of the solid-phase synthesis, the microwaves irradiated from the second end portion 1015b side, that is, the area above the first filter 105, of the first filter 105 are permeated through the first filter 105 having the microwave permeability and are also irradiated to the contents in the area below the first filter 105. The contents in the area below the first filter 105 do not include the solid-phase resin, and thus even if the microwaves are irradiated to the contents in the area below the first filter 105, the processing of the solid-phase synthesis is not performed, and the microwaves are not directly used for the solid-phase synthesis but are wasted.

[0128] In the processing apparatus 1 of the embodiment, the first reflecting member 106 that reflects the microwaves is provided on the first end portion 1015a side, that is, the lower side of the first filter 105. Thus, the microwaves irradiated from the area above the first filter 105 are permeated through the first filter 105 having the microwave permeability, are reflected by the first reflecting member 106 below the first filter 105, and are returned to the area above the first filter 105 in which the contents including the solid-phase resin are held. Thus, the microwaves are not easily irradiated to the area below the first filter 105 in which the solid-phase synthesis is not performed, and the microwaves reflected by the first reflecting member 106 can be used for the solid-phase synthesis. Thus, the microwaves can be efficiently used for the solid-phase synthesis. In particular, when the container 101 is enlarged in order to increase the processing amount of the solid-phase synthesis, the area below the first filter 105 is also enlarged, and thus in the processing apparatus 1 of the embodiment, the waste of the energy can be suppressed and the solid-phase synthesis can be efficiently performed. Further, the first reflecting member 106 has a shape and a size through which the contents can pass through the first filter 105, and thus the first reflecting member 106 does not interfere with the discharge of the contents or the like.

[0129] Further, in the processing device 1 of the present embodiment, the first filter 105 is arranged so as to overlap the first reflecting member 106 made of a reflective material such as metal, and thus the first filter 105 is reinforced and supported by the first reflecting member 106 on the surface, i.e., the upper surface 1061, of the first reflecting member 106 on the side of the second end portion 1015b. Therefore, for example, the first filter 105 made of a low-hardness material or a thin sheet-like first filter 105, which is not easily arranged in the partitioned container 101, can be arranged in the partitioned container 101. This increases the selectivity of the first filter 105.

[0130] An example of a process of solid-phase synthesis using the processing device 1 of the present embodiment will be described below. The process described herein is a process of deprotecting a solid-phase resin to which an N-terminal protected amino acid has been bound, and further binding the amino acid by condensation reaction. However, the substances used in the solid-phase synthesis or the like, or the combination thereof, are examples, and other substances or the like can be used. Further, the flow of the process described herein is an example, and the process can be performed in another flow. Further, the process described herein can be performed in the container 101, and only part of the process described herein can be performed.

[0131] First, in a state in which the first valve 103a is closed and the content is not discharged from the first opening portion 1011, a deprotection solution such as a piperidine solution in DMF or the like, and a solid-phase resin to which an N-terminal protected amino acid such as an Fmoc group has been bound, are supplied to the container 101 through the second opening portion 1012 arranged above the first filter 105, and the lid 1012a is closed. Then, nitrogen gas is supplied to the content in the container 101 through a nozzle or the like not shown in the drawing, and the content is stirred by bubbling, and at the same time, a deprotection process is performed by irradiation of microwaves of 915 MHz from the irradiation device 102. The container 101 is provided with the first filter 105, and thus the deprotection solution and the solid-phase resin are held above the first filter 105, and only the deprotection solution is held in the region below the first filter 105, i.e., the region between the first filter 105 and the first end portion 1015a. However, the deprotection solution moves through the first filter 105 or the first reflecting member 106. The microwaves emitted from the irradiation device 102 are reflected by the first reflecting member 106 to the region 101a above the first reflecting member 106, and thus the microwaves are less likely to be irradiated to the region 101b below the first reflecting member 106, i.e., the region in which the solid-phase resin is not present, i.e., the region that does not need the deprotection process, and the first reflecting member 106 is present below the first filter 105.

[0132] After the deprotection treatment, if the first valve 103a is opened, the deprotection solution below the first filter 105 in the content of the container 101 is discharged to the outside through the first opening portion 1011, the first valve 103a, and the pipe 104, and the deprotection solution above the first filter 105 is discharged to the outside through the first opening portion 1011, the first valve 103a, and the pipe 104 after passing through the first filter 105 and the first reflecting member 106. The deprotected solid-phase resin in the content of the container 101 is separated from the deprotection solution and remains on the upper surface 1051 of the first filter 105.

[0133] After that, DMF is supplied from the second opening portion 1012 into the container 101 and is held, and then the DMF is discharged to wash the deprotected solid-phase resin remaining on the upper surface 1051 of the first filter 105. This washing treatment is performed several times.

[0134] After that, in order to bond an amino acid to the deprotected solid-phase resin, HBTU, DIPEA, an amino acid for bonding, and DMF as a solvent are supplied into the container 101 from the second opening portion 1012, HBTU, DIPEA, and the amino acid for bonding are dissolved in DMF, microwaves are irradiated from the irradiation device 102, and the N-terminal protected amino acid and the deprotected amino acid that has been bonded to the solid-phase resin are bonded in a condensation reaction. As described above, the first filter 105 is provided in the container 101, and therefore, in the region above the first filter 105 in the region 101a above the first reflecting member 106, the bonding solution of HBTU, DIPEA, and the amino acid for bonding dissolved in DMF and the deprotected solid-phase resin are held, and only the bonding solution is held in the region below the first reflecting member 106 and below the first filter 105. However, the bonding solution moves through the first filter 105 and the first reflecting member 106. The microwaves emitted from the irradiation device 102 are reflected by the first reflecting member 106 to the region 101a above the first reflecting member 106, so that the microwaves are less likely to be irradiated to the region 101b below the irradiation device 102 where there is no solid-phase resin, that is, a region where the bonding of the amino acid is not performed.

[0135] After the bonding of the amino acid is completed, if the first valve 103a is opened, the bonding solution is discharged from the container 101, and the solid-phase resin to which the N-terminal protected amino acid is newly bonded is not discharged and remains on the upper surface 1051 of the first filter 105.

[0136] After that, the washing treatment using DMF is repeated several times as described above.

[0137] By this, the peptide to which the solid-phase resin is bonded is obtained on the upper surface 1051 of the first filter 105.

[0138] Further, when the peptide is bonded to a new amino acid, the above-described deprotection treatment and the series of treatments for bonding the amino acid are repeated.

[0139] When the peptide bonded to the solid-phase resin is cleaved from the solid-phase resin remaining on the upper surface 1051 of the first filter 105, for example, the solid-phase resin remaining on the upper surface 1051 of the first filter 105 is treated with trifluoroacetic acid (TFA) and H2O. Also, the cleaved peptide is collected by, for example, precipitating it with diethyl ether or the like, drying, or the like.

[0140] In the above, according to the present embodiment, the microwave is irradiated in the container 101 having microwave reflectivity, whereby the container 101 can be made large and the amount of processing of the peptide or the like by solid-phase synthesis can be increased.

[0141] Also, according to the present embodiment, the first filter 105 is overlaid on the first reflecting member 106, whereby the first reflecting member 106 reflects the microwave that has passed through the first filter 105, the microwave is less likely to be irradiated to the content not containing the solid-phase resin below the first filter 105, and the microwave can be efficiently used for the processing of the solid-phase synthesis.

[0142] Also, according to the present embodiment, the first filter 105 is overlaid on the first reflecting member 106, whereby the first filter 105 can be reinforced by the first reflecting member 106, whereby, for example, the strength or the hardness or the like required for the first filter 105 can be reduced, and the selectivity of the first filter 105 can be increased.

[0143] (Modified Example)

[0144] Figure 3 (a)- Figure 3 The cross-sectional view of (c) is a modified example of the processing apparatus of the present embodiment, and corresponds to the cross-sectional view of (b). Figure 1 (b).

[0145] The above-described embodiment describes the case where the first filter 105 is overlaid on the first reflecting member 106, that is, the side of the second end portion 1015b of the first reflecting member 106, but the first reflecting member 106 can be disposed between the first filter 105 and the first end portion 1015a, preferably between the first filter 105 and the first opening portion 1011, and the first filter 105 can not be directly overlaid on the first reflecting member 106.

[0146] For example, Figure 3(a) shown, the first filter 105 and the first reflecting member 106 can be arranged so as not to overlap directly. In this case, microwaves that have passed through the first filter 105 are reflected by the first reflecting member 106 arranged below the first filter 105, preventing the microwaves from being radiated to the area below the first reflecting member 106, and the microwaves can be efficiently utilized for the solid-phase synthesis. However, in this case, the microwaves are radiated to the contents other than the solid-phase resin between the first filter 105 and the first reflecting member 106, and thus the microwaves cannot be efficiently utilized compared to the case where the first filter 105 overlaps the first reflecting member 106. Also, in this case, the first filter 105 cannot be reinforced by the first reflecting member 106, and thus the first filter 105 needs to be a first filter 105 having sufficient strength even without reinforcement, or a reinforcing member (e.g., a reinforcing frame) having microwave-transmitting properties needs to be additionally provided to the first filter 105, which limits the materials or the structure that can be used as the first filter 105 or complicates the configuration and increases the cost due to the provision of the reinforcing member or the like.

[0147] Also, Figure 1 and Figure 3 The processing device 1 described in (a) can be an example in which the first reflecting member 106 is provided on the side of the first filter 105 closer to the first end portion 1015a, and particularly, can be an example in which the first filter 105 is arranged between the microwave emission position and the first end portion 1015a, and the first reflecting member 106 is provided on the side of the first filter 105 closer to the first end portion 1015a.

[0148] Also, instead of being arranged between the first filter 105 and the first end portion 1015a, the first reflecting member 106 is preferably arranged between the first filter 105 and the first opening portion 1011, as shown in Figure 3 (b) shown, the first reflecting member 106 can be arranged between the first filter 105 and the microwave emission position, and in this case, the microwaves are reflected by the first reflecting member 106, whereby the microwaves are less likely to be radiated to the area below the first filter 105, and the microwaves can be efficiently utilized. However, in this case, in order to allow the solid-phase resin to exist in the area above the first filter 105, the first reflecting member 106 needs to allow the solid-phase resin to pass therethrough. For example, a member having a shape and a size that allow the solid-phase resin to pass therethrough is used as the first reflecting member 106. Also, in this case, the microwaves are less likely to be radiated to the contents containing the solid-phase resin existing between the first reflecting member 106 and the first filter 105, and thus if the distance between the first reflecting member 106 and the first filter 105 becomes wide, the processing efficiency of the solid-phase synthesis can be reduced. Also, in this case, the first filter 105 can be configured of a material other than the microwave-transmitting material.

[0149] Also, as one example when the first reflecting member 106 is arranged between the first filter 105 and the microwave emission position, as shown inFigure 3 As shown in (c), the first reflective member 106 can be overlapped on the upper surface side of the first filter 105. Overlapping the first reflective member 106 on the upper surface side of the first filter 105 allows the first filter 105 to be overlapped on the lower end side of the first reflective member 106. In this case, there is no distance between the first reflective member 106 and the first filter 105, preventing a reduction in the processing efficiency of solid-phase synthesis as described above. The first filter 105 is attached to the lower surface of the first reflective member 106 with an adhesive, or fixed with a fastener or screw (not shown in the figure), thereby reinforcing the first filter 105 with the first reflective member 106. Through the above embodiments or as... Figure 3 (c) Generally, the first filter 105 and the first reflective member 106 are arranged in an overlapping manner, and the first reflective member 106 can reinforce the first filter 105, thereby increasing the selectivity of the first filter 105. In addition, the method of fixing the first reflective member 106 of the first filter 105 described herein can also be used, for example, to fix other filters to other reflective members.

[0150] Figure 3 (b) and Figure 3 The processing device 1 described in (c) may be a case where the first filter 105 is disposed between the first end 1015a and the microwave emission position, and is an example where the first reflective member 106 is provided between the first filter 105 and the microwave emission position.

[0151] Furthermore, as can be seen from the above embodiments or their variations, in the processing apparatus 1, as long as the first opening 1011 is provided at the lower part of the container 101, the first filter 105 is disposed between the first opening 1011 and the second end 1015b in a manner that separates the container 101 so that solid substances such as solid resin can be separated from the contents, and the first reflective member 106 is disposed between the irradiation position and the first opening 1011 in a manner that separates the container 101 so that microwaves are less likely to irradiate the side of the first reflective member 106 that is closer to the first end 1015a.

[0152] Also, such as Figure 1 and Figure 3As illustrated in FIG. 1, the processing device 1 according to the present embodiment and its modified examples is configured such that the first filter 105 is disposed between the first opening portion 1011 and the microwave emission position, and the first reflecting member 106 is disposed between the first opening portion 1011 and the irradiation position. Therefore, the first opening portion 1011 of the processing device 1 can be disposed on the side of the first end portion 1015a of the container 101, which is closer to the first filter 105 and the first reflecting member 106. The first opening portion 1011 is disposed on the side of the first end portion 1015a of the container 101, which is closer to the first filter 105 and the first reflecting member 106, for example, means that the first opening portion 1011 is disposed on the side of the first end portion 1015a of the container 101, which is closer to both the first filter 105 and the first reflecting member 106. Further, the first opening portion 1011a described later is disposed on the side of the first end portion 1015a of the container 101, which is closer to the first filter 105 and the first reflecting member 106, the second opening portion 1011b is disposed on the side of the second end portion 1015b of the container 101, which is closer to the second filter 107 and the second reflecting member 108, the second filter 107 is disposed on the side of the first filter 105 and the first reflecting member 106, which is closer to the second end portion, the second reflecting member 108 is disposed on the side of the first filter 105 and the emission position, which is closer to the second end portion 1015b, the guide inlet 5013 is disposed on the side of the first filter 502 and the first reflecting member 503, which is closer to the second end portion 5011b of the region of the side surface of the cylindrical member 501, and the guide inlet 5013 is disposed on the side of the second filter 504 and the second reflecting member 505, which is closer to the first end portion 5011a of the region of the side surface of the cylindrical member 501, and the like are also explained in the same manner as the above.

[0153] Further, as described above, the first opening portion 1011 can be disposed on the first end portion 1015a of the container 101, and the like. In this case, the processing device 1 can be configured such that the first filter 105 is disposed between the first end portion 1015a and the emission position so as to partition the container 101, the first reflecting member 106 is disposed between the first end portion 1015a and the irradiation position so as to partition the container 101, and the first opening portion 1011 is disposed on the side of the first end portion 1015a of the container 101, which is closer to the first filter 105 and the first reflecting member 106.

[0154] Further, as described above, the first opening portion 1011 can be disposed on the first end portion 1015a of the container 101, and the like. In this case, the processing device 1 can be configured such that the first filter 105 is disposed between the first end portion 1015a and the emission position so as to partition the container 101, the first reflecting member 106 is disposed between the first end portion 1015a and the irradiation position so as to partition the container 101, and the first opening portion 1011 is disposed on the side of the first end portion 1015a of the container 101, which is closer to the first filter 105 and the first reflecting member 106. Figure 3(a) In the modification shown in the figure, the first reflecting member 106 is disposed on the side of the first end portion 1015a relative to the first filter 105, and when the first filter 105 is disposed separately from the first reflecting member 106, the irradiation device 102 can irradiate microwaves into the container 101 from a position between the first filter 105 and the first reflecting member 106 of the container 101. That is, the irradiation position can be set to a position between the first filter 105 and the first reflecting member 106 of the container 101. In this case, the solid substance such as the solid-phase resin that is the separation target is supplied, for example, to a position between the first filter 105 and the second end portion 1015b above the first filter 105, and the contents are supplied, for example, to a position higher than the first filter 105 in the container 101. In this configuration, the first filter 105 is also disposed between the first end portion 1015a and the second end portion 1015b of the container 101, and the first reflecting member 106 is disposed between the first end portion 1015a and the irradiation position. In this configuration, microwaves are less likely to be irradiated to a region below the first reflecting member 106, and the same effects as described above can be achieved. In this case, when the contents are in a liquid state, microwaves are irradiated, for example, from the liquid. In addition, the position between the first filter 105 and the first reflecting member 106 of the container 101 means, for example, a position in the height direction of the container 101 that is between the first filter 105 and the first reflecting member 106. For example, the irradiation opening portion 1013 is provided at a position between the first filter 105 and the first reflecting member 106 of the container 101, and the irradiation device 102 irradiates microwaves into the container 101 from the irradiation opening portion 1013.

[0155] In addition, in the processing device in which the irradiation position is set to a position between the first filter 105 and the first reflecting member 106, and the processing device according to the embodiment or the modification described above, in the processing device 1, the first opening portion 1011 is provided on the side of the first end portion 1015a of the container 101, the first filter 105 is disposed between the first opening portion 1011 and the second end portion 1015b in a manner of partitioning the container 101, so that the solid substance such as the solid-phase resin can be separated from the contents, and the first reflecting member 106 is disposed between the irradiation position and the first opening portion 1011 in a manner of partitioning the container 101, so that microwaves are less likely to be irradiated to the side of the first end portion 1015a relative to the first reflecting member 106.

[0156] Also, as described above, from the fact that the first opening portion 1011 can be provided at the first end portion 1015a of the container 101 or the like, in the processing apparatus 1, for example, the first filter 105 can be disposed in a manner of partitioning the container 101, and at the same time, the first reflection member 106 can be disposed in a manner of partitioning the container 101 on the first end portion 1015a side from the irradiation position. However, in this case, the first filter 105 and the first reflection member 106 are disposed separately from the first opening portion 1011. For example, in the processing apparatus 1, as long as the first filter 105 is disposed in a manner of partitioning the container 101 between the first end portion 1015a and the second end portion 1015b, the first reflection member 106 is disposed in a manner of partitioning the container 101 between the irradiation position and the first end portion 1015a of the container 101, and the first opening portion 1011 is provided on the first end portion 1015a side from the first filter 105 and the first reflection member 106, the same effects as described above can be obtained in this case as well.

[0157] Further, the irradiation opening portion 1013 described in the above embodiment can be, for example, an opening portion used to irradiate microwaves into the container 101. The irradiation opening portion 1013 can be, for example, an introduction port to introduce microwaves into the container 101 or a portion to emit microwaves into the container 101, that is, a microwave exit portion, or the like. The same applies to other embodiments.

[0158] Also, the irradiation position described in the above embodiment can be, for example, the position of the irradiation opening portion 1013, or the position of the above-described exit portion. Also, the positional relationship of the first filter 105, the first reflection member 106 described later, the second filter 107, the second reflection member 108, or the like with respect to the irradiation position can be alternatively interpreted as the positional relationship with respect to the irradiation opening portion 1013 or the exit portion. The same applies to other embodiments.

[0159] (Embodiment 2)

[0160] The processing apparatus of the present embodiment is a processing apparatus in which filters that reflect microwaves are used instead of the first filter and the first reflection member in the processing apparatus described in the above Embodiment 1.

[0161] Figure 4 is a perspective view showing an example of the processing apparatus in the present embodiment (a), and a cross-sectional view taken along the line IVb-IVb thereof (b). However, Figure 4 The cross section of the valve and the like is omitted in (b). Figure 4 Figure 4

[0162] ​​The processing device 2 of this embodiment includes the container 101, the irradiation device 102, the first valve 103, and the first reflection filter 205. The container 101 includes the first opening portion 1011, the second opening portion 1012, and the irradiation opening portion 1013. The other components of the first reflection filter 205 are the same as those of the first embodiment, and thus detailed description thereof is omitted.

[0163] The first reflection filter 205 is a filter in which the first filter 105 and the first reflection member 106 are integrated, and is a filter that separates the solid-phase resin from the contents in the container 101 and reflects microwaves irradiated by the irradiation device 102 into the container 101. The first reflection filter 205 is a filter made of stainless steel, which is a microwave-reflective material, and has a plurality of holes for separating the solid-phase resin from the contents in the container 101. However, the first reflection filter 205 can be made of a microwave-reflective material other than stainless steel, as long as it reflects microwaves and can separate the solid-phase resin from the contents in the container 101. The first reflection filter 205 is preferably made of a chemically stable material having excellent corrosion resistance. For example, the first reflection filter 205 can be a metal filter having a coating of a material such as PTFE, which has corrosion resistance and microwave permeability, or the like. The size and the like of the plurality of holes of the first reflection filter 205 for separating the solid-phase resin from the contents in the container 101 are the same as those of the holes of the first filter 105 of the above-described embodiment, and thus detailed description thereof is omitted. Known metal filters include, for example, a metal mesh made of stainless steel or the like, or a metal filter in which a plurality of metal meshes are integrated by sintering.

[0164] The first reflection filter 205 is a member in which the first filter 105 and the first reflection member 106 are integrated, and thus can be disposed at a position at which the first filter 105 can be disposed and at a position at which the first reflection member 106 can be disposed. Therefore, the first reflection filter 205 is disposed between the first end portion 1015a and a position at which microwaves are emitted by the irradiation device 102, in a manner that separates the container 101, as with the first reflection member 106 of the above-described embodiment. In addition, the first opening portion 1011 can be provided on the side of the lower end portion of the first reflection filter 205, that is, on the side of the first end portion 1015a.

[0165] In addition, when the first reflection filter 205 is a metal filter made of stainless steel or the like, the filter can be disposed alone in the container 101, but the hardness or the strength can be insufficient, and thus it can be difficult to dispose the first reflection filter 205 alone in the container 101. In this case, a frame or the like for reinforcement can be attached to the first reflection filter 205. The same applies to the second reflection filter 206, which will be described later.

[0166] In this embodiment as well, as in the above embodiment, the container 101 can be made large and the amount of peptides and the like subjected to solid-phase synthesis can be increased. Further, by providing the first reflective filter 205, the solid-phase resin can be separated from the contents. Further, in the region below the first reflective filter 205 in the container 101, the solid-phase resin is prevented from moving above the first reflective filter 205, and the region is made to contain no solid-phase resin. By the first reflective filter 205, microwaves irradiated by the irradiation device 102 are reflected to the region above the first reflective filter 205, whereby the microwaves are less likely to be irradiated to the contents containing no solid-phase resin below the first reflective filter 205, and the microwaves can be efficiently used for the process of solid-phase synthesis.

[0167] (Embodiment 3)

[0168] The process device of this embodiment is the process device described in Embodiment 1, further provided with a second filter identical to the first filter and a second reflective member identical to the first reflective member on the second end portion side in the container.

[0169] Figure 5 is a perspective view of an example of a process device in this embodiment (a) and a sectional view of Vb-Vb line thereof (b). However, Figure 5 (a) and (b) omit the sectional view of the valve. Figure 5 Figure 5

[0170] The process device 3 is provided with a container 301, an irradiation device 302, a first valve 103a, a second valve 103b, a first filter 105, a first reflective member 106, a second filter 107, and a second reflective member 108. The container 301 has a first opening portion 101 la, a second opening portion 101 lb, a third opening portion 101 lc, and an irradiation opening portion 1013. The same symbols as in Figure 1 The same symbols as in

[0171] In this embodiment as well, as in the above embodiment, the container 101 can be made large and the amount of peptides and the like subjected to solid-phase synthesis can be increased. Further, by providing the first reflective filter 205, the solid-phase resin can be separated from the contents. Further, in the region below the first reflective filter 205 in the container 101, the solid-phase resin is prevented from moving above the first reflective filter 205, and the region is made to contain no solid-phase resin. By the first reflective filter 205, microwaves irradiated by the irradiation device 102 are reflected to the region above the first reflective filter 205, whereby the microwaves are less likely to be irradiated to the contents containing no solid-phase resin below the first reflective filter 205, and the microwaves can be efficiently used for the process of solid-phase synthesis.

[0172] ​​The container 301 is a container for one or more processes of solid-phase synthesis inside. The container 301 has a first end portion 1015a and a second end portion 1015b. Here, as with the container 101, a case where the container 301 is a vertical container and the first end portion 1015a of the container 301 is the lower end portion of the container 301 and the second end portion 1015b is the upper end portion is exemplified and described.

[0173] Further, the container 301 has a first opening portion 1011a, a second opening portion 1011b, and a third opening portion 1011c instead of the first opening portion 1011 and the second opening portion 1012, and the irradiation device 302 emits microwaves to a position inside the container 301, i.e., an emission position is between the first end portion 1015a and the second end portion 1015b, and the like, and is the same as the container 101 of the above-described Embodiment 1 except the above, and thus detailed description is omitted here.

[0174] Further, the container 301 can also have a stirring device (not shown) for stirring the contents inside, as with the container 101 of the above-described Embodiment 1. Also, the container 301 can also have a device (not shown) for measuring the temperature inside the container 301, such as a temperature sensor, or a sensor other than the temperature sensor, such as a pressure sensor, inside or outside the container 101, as with the container 101. Also, the temperature inside the container 301 or the output or frequency of the microwaves emitted to the container 301 can be feedback-controlled using the value obtained by the sensor or the like. Also, the container 301 can have an observation window (not shown) for observing the inside of the container 301, as with the container 101. Also, the container 301 can be a container whose internal pressure can be changed. For example, the container 301 can be a container whose internal pressure can be reduced or increased, as with the container 101.

[0175] The irradiation device 302 irradiates microwaves into the container 301 from a position between the first end portion 1015a and the second end portion 1015b of the container 301. Here, the irradiation device 302 irradiates microwaves into the container 301 from a position between the lower end portion and the upper end portion of the container 301. Specifically, the irradiation device 302 irradiates microwaves into the container 301 from a position in the longitudinal direction of the container 301 between the first end portion 1015a and the second end portion 1015b of the container 301. Here, a case where the irradiation device 302 has a combination of one set of microwave oscillator 1021 and a waveguide 1022 connected to the microwave oscillator 1021 at one end portion, and irradiates microwaves into the container 301 from one position is described. Specifically, the waveguide 1022 of the irradiation device 302 is attached to the container 301 in a manner to communicate with the irradiation opening portion 1013 of the container 301, and the irradiation device 302 irradiates microwaves into the container 301 through the irradiation opening portion 1013. The irradiation opening portion 1013 is provided at a position in the longitudinal direction of the container 301 between the first end portion 1015a and the second end portion 1015b of the container 301. The position in the longitudinal direction of the container 301 can be a position in the height direction of the container 301. Here, a case where the irradiation opening portion 1013 is provided on the side surface of the container 301 near the center in the height direction of the container 301 is described. However, the position of the irradiation opening portion 1013 is not limited as long as the irradiation device attached to the irradiation opening portion 1013 can irradiate microwaves into the container 301 from a position between the first end portion 1015a and the second end portion 1015b of the container 301. Also, here, a case where the waveguide 1022 is attached to the side surface of the container 301 in a manner that the axial direction of the waveguide 1022 is perpendicular to the longitudinal direction of the container 301 is described, but the angle at which the waveguide 1022 is attached to the side surface of the container 301 is not limited. For example, the waveguide 1022 can be attached in a manner that the axial direction is inclined to the longitudinal direction of the container 301. As for the irradiation device 302 or the microwave oscillator 1021 and the waveguide 1022 of the irradiation device 302, the same as the irradiation device 102 of the above-described Embodiment 1 is used except for the position at which microwaves are irradiated into the container 301 and the like, and detailed description thereof is omitted here.

[0176] Further, the irradiation device 302 can be a member other than the irradiation device 102 of the above-described Embodiment 1 as long as it can irradiate microwaves into the container 301. Also, for example, the irradiation device 302 is not limited to irradiate microwaves into the container 301 from one position, but can irradiate microwaves into the container 301 from one or more positions between the first end portion 1015a, i.e., the lower end portion, and the second end portion 1015b, i.e., the upper end portion of the container 301. The configuration to irradiate microwaves from multiple positions can use the same configuration as the irradiation device 102 described in the above-described Embodiment 1, and detailed description thereof is omitted here.

[0177] Further, the irradiation device 302 is a member that irradiates microwaves of a different frequency into the container 301, like the irradiation device 102 of Embodiment 1 described above.

[0178] The first filter 105 is disposed between the first end portion 1015a of the container 301 and the position at which the irradiation device 302 irradiates microwaves, in a manner that partitions the container 301. The first filter 105 is disposed at a position in the length direction (in this case, the height direction) of the container 301 that is away from the first end portion 1015a. By the first filter 105 being disposed between the first end portion 1015a and the irradiation position, it is meant, for example, that the first filter 105 is disposed in a manner such that the position of the first filter 105 in the length direction of the container 301 is between the first end portion 1015a and the irradiation position. Further, the first filter 105 is the same as the first filter 105 of Embodiment 1 described above, except for the above point, and thus detailed description thereof is omitted here.

[0179] Further, the first filter 105 is preferably disposed at a position at a height of 1 / 4 or less of the height of the container 301, in terms of sufficiently ensuring the region in which the solid phase growth process is performed, like Embodiment 1 described above, but the first filter 105 can also be disposed at a position at a height of more than 1 / 4.

[0180] The first reflection member 106 is disposed between the first end portion 1015a of the container 301 and the irradiation position, in a manner that partitions the container 301. The first reflection member 106 is disposed at a position in the length direction (in this case, the height direction) of the container 301 that is away from the first end portion 1015a. By the first reflection member 106 being disposed between the first end portion 1015a and the irradiation position, it is meant, for example, that the first reflection member 106 is disposed in a manner such that the position of the first reflection member 106 in the length direction of the container 301 is between the first end portion 1015a and the irradiation position. Here, an example is shown in which the first reflection member 106 is disposed between the first filter 105 and the first end portion 1015a of the container 301, in a manner that partitions the container 301. Here, in particular, an example is shown in which the first filter 105 is disposed on the first reflection member 106, that is, on the side of the second end portion 1015b of the first reflection member 106. Further, the first reflection member 106 is the same as the first reflection member 106 of Embodiment 1 described above, except for the above point, and thus detailed description thereof is omitted here.

[0181] The second filter 107 is disposed between the second end portion 1015b of the container 301 and the discharge position in a manner partitioning the container 301. The second filter 107 is disposed at a position away from the second end portion 1015b in the length direction (in this case, the height direction) of the container 301. By the second filter 107 being disposed between the second end portion 1015b and the discharge position, for example, the second filter 107 is disposed in a manner such that the position of the second filter 107 in the length direction of the container 301 is between the second end portion 1015b and the discharge position. The second filter 107 is disposed in a manner partitioning the container 301, for example, in the same manner as when the first filter 105 is disposed in a manner partitioning the container 101 or the container 301.

[0182] The second filter 107 is a member that separates the solid-phase resin used in the solid-phase synthesis from the contents in the container 301. The second filter 107 can use the same member as the first filter 105 described above, and thus detailed description thereof is omitted here. In addition, the second filter 107 can be the same as the first filter 105, or can be different. For example, the material of the second filter 107 can be the same material as the first filter 105, or can be a different material, as long as the material is a microwave-transmissive material. Also, for example, the plurality of pores of the second filter 107 can be the same size or shape as the pores of the first filter 105, or can be different, as long as the plurality of pores are of a size or shape that separates the solid-phase resin used in the solid-phase synthesis from the contents in the container 301.

[0183] In addition, the second filter 107 is preferably disposed at a position at a height of 3 / 4 or more of the height of the container 301, or can be disposed at a position lower than 3 / 4, in order to sufficiently secure a region in which the solid-phase growth process is performed.

[0184] The second reflection member 108 is disposed between the second end portion 1015b of the container 301 and the discharge position in a manner partitioning the container 301. The second reflection member 108 is disposed at a position away from the second end portion 1015b in the length direction (in this case, the height direction) of the container 301. Here, an example is described in which the second reflection member 108 is disposed in a manner partitioning the container 301 between the second filter 107 and the second end portion 1015b in the container 301. Here, an example is particularly described in which the second reflection member 108 is disposed in a manner overlapping the second filter 107, that is, on the side of the second end portion 1015b of the second filter 107.

[0185] The second reflection member 108 is disposed, for example, so as to be above the second filter 107, that is, on the side of the second end portion 1015b of the second filter 107, with respect to the microwave emitted from the emission position into the container 301, and is disposed, except for the different point, in the same manner as the first reflection member 106 so as to partition the container 301.

[0186] Like when the first filter 105 is disposed so as to overlap the first reflection member 106, the second reflection member 108 is disposed so as to overlap the second filter 107, that is, on the side of the second end portion 1015b of the second filter 107. Further, the second filter 107 is disposed so as to overlap the second reflection member 108 on the side of the first end portion 1015a, and therefore the second filter 107 is preferably fixed to the side of the first end portion 1015a of the second reflection member 108. The fixing can be performed in any manner, for example, when the second filter 107 is a thin filter, or when the second filter 107 is not sufficiently hard, the second filter 107 can be adhered to the side of the second reflection member 108 with an adhesive or the like, or a fixture (not shown) or the like can be used to fix the second filter 107 so as not to fall to the side of the second reflection member 108.

[0187] The second reflection member 108 allows the contents in the container 301, for example, the contents after separation of the solid-phase resin used for synthesis by the second filter 107, to pass therethrough, and reflects the microwaves emitted from the emission device 302. The material of the second reflection member 108 is, for example, a material having microwave reflectivity, that is, stainless steel. The second reflection member 108 has, for example, a plurality of holes 108a having a circular planar shape, and the plurality of holes 108a are sized to allow the contents in the container 301 to pass therethrough. The second reflection member 108 can be formed using the same member as the first reflection member 106 described above, and therefore detailed description thereof is omitted here.

[0188] Further, the second reflection member 108 can be the same as the first reflection member 106, or can be different. For example, the material of the second reflection member 108 can be the same material as the first reflection member 106, or can be a different material, as long as the material has microwave reflectivity. Also, for example, the plurality of holes of the second reflection member 108 can have the same size or shape as the first reflection member 106, or can have a different size or shape, as long as the plurality of holes are sized or shaped to allow the contents from which the solid-phase resin used for synthesis is removed to pass therethrough.

[0189] A first opening portion 1011a is provided on the container 301 on the side of the first filter 105 and the first reflecting member 106 closer to the first end portion 1015a. That is, the first opening portion 1011a is provided on the side of the first filter 105 closer to the first end portion 1015a and on the side of the first reflecting member 106 closer to the first end portion 1015a. The first opening portion 1011a is an opening portion for discharging the contents of the container 301. The contents discharged here can not discharge all of the contents. The contents in the container 301 are, for example, the substances, solvents, and the like used in the solid-phase synthesis described above, or the cleaning liquid (for example, a solvent), and the like. The liquid contents include solutions, suspensions, and the like. The contents discharged from the first opening portion 1011a are, for example, the portion of the contents held in the container 301 before discharge that is filtered by the first filter 105 described below, and the portion of the contents from which the solid-phase resin separated by filtration is removed. Here, an example in which one first opening portion 1011a is provided is described, but a plurality of first opening portions 1011a can be provided. The first opening portion 1011a is preferably provided at the lowermost portion of the container 301 in a manner that the contents in the container 301 are naturally discharged. The size and shape of the first opening portion 1011a are not limited. The first opening portion 1011a can be, for example, the same opening portion as the first opening portion 1011.

[0190] Further, the size of the first opening portion 1011a is preferably smaller than the size of the portion of the container 101 away from the first end portion 1015a (for example, the portion near the center in the length direction or the portion in which the first filter 105 or the first reflecting member 106 is provided). The portion away from the first end portion 1015a is, for example, the portion in the length direction of the container 101 away from the first end portion 1015a. The portion away from the first end portion 1015a can be the portion away from the first opening portion 1011a.

[0191] The second opening portion 1011b is provided on the side of the second end portion 1015b, i.e., the upper end portion side, of the container 301, relative to the second filter 107 and the second reflection member 108. That is, the second opening portion 1011b is provided at a position on the side of the second end portion 1015b relative to the second filter 107 and on the side of the second end portion 1015b relative to the second reflection member 108. The second opening portion 1011b is an opening portion for supplying a content into the container 301. The content supplied from the second opening portion 1011b is, for example, a substance or a solvent used for solid-phase synthesis, a cleaning liquid, a gas, or the like. The content supplied from the second opening portion 1011b can be all or a part of the content supplied into the container 301. The content supplied from the second opening portion 1011b is, for example, a content that can pass through the second filter 107. The content supplied from the second opening portion 1011b is, for example, a substance or a solvent used for solid-phase synthesis other than the solid-phase resin, a cleaning liquid, a gas, or the like. In this embodiment, an example in which one second opening portion 1011b is provided is described, but a plurality of second opening portions 1011b can be provided. The size and shape of the second opening portion 1011b are not limited. The second opening portion 1011b can be blocked by a cap, a lid, a plug, or the like, not shown in the drawing, when the content is not supplied, when the second opening portion 1011b does not have a second valve 103b or the like described later. The second opening portion 1011b can be, for example, an opening portion having the same configuration or arrangement as the second opening portion 1012. However, the second opening portion 1011b is different from the second opening portion 1012, and is not generally used for supplying the solid-phase resin separated by the first filter 105 and the second filter 107.

[0192] Further, the size of the second opening portion 1011b is preferably smaller than the size of a portion of the container 101 away from the second end portion 1015b, such as a portion near the center in the length direction or a portion in which the second filter 107 or the second reflection member 108 is provided. The portion away from the second end portion 1015b is, for example, a portion of the container 101 away from the second end portion 1015b in the length direction. The portion away from the second end portion 1015b can be a portion away from the second opening portion 1011b.

[0193] A third opening 1011c is provided in the region between the first filter 105 and the first reflective member 106 and the second filter 107 and the second reflective member 108 in container 301. The region between the first filter 105 and the first reflective member 106 and the second filter 107 and the second reflective member 108 refers, for example, the region between both the first filter 105 and the first reflective member 106 and both the second filter 107 and the second reflective member 108. Similarly, the region between the first filter 105 and the first reflective member 106 and the second filter 107 and the second reflective member 108 refers to the region between the portion of the first filter 105 and the first reflective member 106 near the second end 1015b and the portion of the second filter 107 and the second reflective member 108 near the first end 1015a. The third opening 1011c is an opening within container 301 used to supply substances or solvents used in solid-phase synthesis, cleaning liquids, gases, etc., into container 301. The contents supplied through the third opening 1011c may be, for example, substances or solvents used in solid-phase synthesis, cleaning liquids, gases, etc. The contents supplied through the third opening 1011c may be all or a portion of the contents supplied to the container 301. The contents supplied through the third opening 1011c may be, for example, contents that cannot pass through the first filter 105 and the second filter 107. The contents supplied through the third opening 1011c may be, for example, solid-phase resin or substances or solvents used in solid-phase synthesis containing solid-phase resin. For example, in the area within the container 301 separated by the first filter 105 and the second filter 107, solid-phase resin cannot be supplied through the second opening 1011b; therefore, solid-phase resin is supplied through the third opening 1011c in that area. This example illustrates the provision of one third opening 1011c, but multiple third openings 1011c may also be provided. The size and shape of the third opening 1011c are not limited.

[0194] The third opening 1011c is preferably blocked by a cap or plug (not shown) when not in use. The piping (not shown) for supplying the contents of the container 301 to the third opening 1011c can be connected via a valve (not shown). Furthermore, the third opening 1011c may be equipped with one or more nozzles (not shown), which are used to supply the contents into the container 301 and extend into it. These nozzles can be detachable components. The case where the third opening 1011c is closed with an openable / closable cap 1012a is shown here.

[0195] The first opening 1011a of container 301 is equipped with a first valve 103a, similar to the first opening 1011 described above. The first valve 103a is further connected to a piping 104a. The first valve 103a is used as a discharge device to control the discharge of contents from the container 301 through the first opening 1011a. The first valve 103a is the same as the first valve 103a described in Embodiment 1 above, so a detailed description is omitted here. When the contents of container 301 are discharged directly through the first valve 103a, the piping 104a can be omitted. Furthermore, the first valve 103a can be directly installed in the first opening 1011a as described above, or it can be indirectly installed through piping or the like, which is not shown in the figure.

[0196] Furthermore, similar to Embodiment 1 described above, other discharge devices besides the first valve 103a can be provided to replace the first valve 103a. In addition, the discharge devices such as the first valve 103a can be part of the processing device 3, or they can be installed outside the processing device 3 without being part of the processing device 3.

[0197] A second valve 103b is installed at the second opening 1011b of container 301. The second valve 103b is further connected to a pipe 104b. The second valve 103b is used as a supply device to control the supply of contents from the second opening 1011b to container 301. Opening the second valve 103b allows the contents to be supplied to container 301 through the pipe 104b connected to the second valve 103b. Closing the second valve 103b stops the supply of contents to container 301. The second valve 103b uses the same components as the first valve 103a. When the contents are supplied directly to container 301 through the second valve 103b, the pipe 104b can be omitted. Furthermore, the second valve 103b can be directly installed at the second opening 1011b as described above, or it can be indirectly installed through a pipe (not shown in the figure).

[0198] Furthermore, while this embodiment describes the installation of a second valve 103b, any supply device other than the second valve 103b can be installed on the second opening 1011b of the container 301 and capable of controlling the supply of contents into the container 301. Also, when the contents are directly supplied into the container 301 through the second opening 1011b, the second valve 103b may not be required. In this case, a plug or cap that can open and close the second opening 1011b may be provided. Furthermore, for example, a pipe 104b can be directly connected to the second opening 1011b, and the contents can be supplied into the container 301 via the pipe 104b. In addition, this embodiment exemplifies the case where the second valve 103b is part of the processing device 3, but the second valve 103b and other supply devices may be part of the processing device 3 or may be installed outside the processing device 3.

[0199] In the processing apparatus 3 of this embodiment, microwaves are irradiated by an irradiation device 302 within a container 301 made of a microwave-reflective material. This encloses the microwaves within the container 301, allowing for efficient irradiation of the contents. Furthermore, the microwaves within the container 301 can be multimode, which, compared to single-mode irradiation, prevents the microwaves from concentrating in one place and allows for more even irradiation of the contents. Therefore, even with a larger container 301, microwaves can be efficiently and evenly irradiated onto the contents. Thus, in this embodiment, the container 301 can be enlarged, and the processing capacity of peptides and the like synthesized via solid-phase synthesis can be increased.

[0200] Furthermore, the processing apparatus 3 of this embodiment includes a first filter 105, which allows solid resin to be separated from the contents supplied between the first filter 105 and the second filter 107 and remain in the container 301. By supplying a cleaning solvent to the container 301 for separating the solid resin and cleaning the solid resin, or by supplying other materials, solvents, or solutions to the container 301 for separating the solid resin, other solid-phase synthesis processes can be performed without temporarily removing the solid resin.

[0201] Furthermore, in the processing apparatus 3 of this embodiment, a first reflective member 106 for reflecting microwaves is provided on the lower side of the first end 1015a side of the first filter 105. This allows microwaves irradiated from above the first filter 105 to pass through the microwave-permeable first filter 105, but be reflected by the first reflective member 106 below it, returning to the area above the first filter 105 where the contents containing the solid resin are held. Therefore, microwaves are less likely to irradiate the area below the first filter 105 where solid-phase synthesis is not performed, and the microwaves reflected by the first reflective member 106 can also be used for solid-phase synthesis, thus efficiently utilizing microwaves for solid-phase synthesis. This allows the processing apparatus 3 of this embodiment to suppress excess energy consumption and efficiently perform solid-phase synthesis. Furthermore, the first reflective member 106 has a shape and size that allows the contents of the first filter 105 to pass through, so the first reflective member 106 does not obstruct the discharge of the contents.

[0202] Furthermore, for example, if the materials used in solid-phase synthesis are supplied into the container without reaching the top, and microwaves are applied to the container to perform solid-phase synthesis while the top side is empty, the microwaves will only reach the top side, which lacks the materials used in solid-phase resin synthesis. Since the top side does not contain the materials for solid-phase resin synthesis, the microwaves applied to that area do not directly contribute to solid-phase synthesis and will waste energy. Especially when the container 301 is enlarged to increase the solid-phase synthesis throughput, the area on the top side without materials also becomes larger, making it impossible to efficiently utilize the microwaves.

[0203] In this embodiment, the processing device 3 is provided with a second filter 107 and a second reflector 108. Therefore, the microwaves irradiated by the microwave irradiation device will be reflected by the second reflector 108, making it difficult to irradiate the area on the side closer to the second end 1015b of the second reflector 108, that is, the area on the upper end side. This prevents the microwaves from irradiating the area on the upper end side of the container 301, which does not contain any contents and does not undergo solid-phase synthesis, and allows for efficient use of the microwaves. Furthermore, since the second filter 107 is disposed between the microwave irradiation position and the second reflective member 108, it is assumed that even if the contents of the container 301 are filled to a position higher than the second reflective member 108, the solid resin supplied between the first filter 105 and the second filter 107 will not move to the area above the second filter 107, that is, the area on the side of the second end 1015b. Moreover, the solid resin will not exist in the area between the second reflective member 108 and the second end 1015b in the container 301, which is not easily irradiated by microwaves. Therefore, the solid resin exists in the area between the first reflective member 106 and the second reflective member 108 that irradiate microwaves in the container 301, which can reduce the amount of solid resin that does not contribute to solid-phase synthesis and efficiently carry out solid-phase synthesis.

[0204] Furthermore, solid resin is supplied to the region between the first filter 105 and the second filter 107 and exists in this region. It can reflect microwaves between itself and the first reflective member 106 and the second reflective member 108, which are arranged in an overlapping manner. By adjusting the installation positions of the first filter 105 and the second filter 107 within the container 301, the region where solid resin exists can be set. This region can be designated as the area to be irradiated with microwaves and solid-phase synthesis can be performed. Therefore, even without changing the dimensions of the container 301, the size of the region where solid resin exists within the container 301 can be set to the desired size, and solid-phase synthesis can be performed in this region, avoiding unnecessary energy consumption. For example, the size of the region where solid resin exists can be set according to the number of solid resins used in solid-phase synthesis, and microwaves are irradiated almost exclusively in this region. This allows the region for solid-phase synthesis to be set to a size suitable for the number of solid resins, thus avoiding unnecessary energy consumption.

[0205] Furthermore, in the processing apparatus 3 of this embodiment, similarly to embodiment 1, the first filter 105 is overlapped and disposed on the first reflective member 106 made of a reflective material such as metal. Therefore, the first filter 105 can be reinforced and supported by the first reflective member 106. The first filter 105 is disposed on the surface of the second end 1015b side of the first reflective member 106, i.e., the upper surface 1061. This increases the selectivity of the first filter 105.

[0206] Furthermore, in the processing apparatus 3 of this embodiment, a second reflective member 108 made of a reflective material such as metal is overlapped with the second filter 107. Therefore, the second filter 107 can be reinforced and supported by the second reflective member 108, which is disposed on the surface of the second reflective member 108 at its first end 1015a, i.e., the lower surface side. Thus, for example, a second filter 107 made of a low-hardness material that is not easily disposed separately in the partition container 301, or a sheet-like second filter 107, can be disposed within the partition container 301. This increases the selectivity of the second filter 107.

[0207] The following describes an example of solid-phase synthesis processing using the processing apparatus 3 of this embodiment. This description focuses on a case where the same solid-phase synthesis process as described in Embodiment 1 is performed. However, the substances used in this solid-phase synthesis, or their blending, are only one example; other substances may also be used. Furthermore, the processing flow described here is also an example; other processes may also be used. Additionally, solid-phase synthesis processes other than those described herein may be performed within container 301, or only a portion of the processes described herein may be performed.

[0208] First, with the first valve 103a closed, a solid resin containing amino acids with N-terminal protection such as Fmoc groups bonded to it is supplied through the third opening 1011c between the first filter 105 and the second filter 107 inside the container 301, and the cover 1012a is closed. Then, a deprotection solution, such as a piperidine solution using DMF as a solvent, is supplied through the second opening 1011b of the container 301. Inside the container 301, the deprotection solution is supplied to a position higher than the second reflector 108. Next, nitrogen gas is supplied to the contents of the container 301 through a nozzle (not shown) and agitated with bubbles, and the contents are irradiated with 915MHz microwaves by the irradiation device 302 for deprotection treatment. The deprotection solution and solid resin are maintained between the first filter 105 and the second filter 107 of the container 301, while only the deprotection solution is maintained in the area below the first filter 105 and above the second filter 107. However, the deprotection solution moves through the first filter 105, the first reflective member 106, the second filter 107, and the second reflective member 108. The microwaves emitted by the irradiation device 302 are reflected in the area between the first reflective member 106 and the second reflective member 108, and between the first reflective member 106 and the second reflective member 108. This prevents the microwaves from easily irradiating the areas below the first filter 105 and above the second filter 107 where there is no solid resin, i.e., the areas that do not require deprotection treatment.

[0209] After the deprotection treatment is completed, if the first valve 103a is opened, the deprotection solution above the second reflective member 108 in the contents of container 301 will be discharged to the outside through the second reflective member 108, the second filter 107, the first filter 105, the first reflective member 106, the first opening 1011a, the first valve 103a, and the pipe 104a. The deprotection solution between the first filter 105 and the second filter 107 will be discharged to the outside through the first filter 105, the first reflective member 106, the first opening 1011a, the first valve 103a, and the pipe 104a. The deprotection solution below the first reflective member 106 will be discharged to the outside through the first opening 1011a, the first valve 103a, and the pipe 104a. In the contents of container 301, the deprotected solid resin will be separated by the deprotection solution and remain on the upper surface 1051 of the first filter 105.

[0210] Next, the second valve 103b is opened, and DMF supplied via piping 104b is supplied into container 301 through the second opening 1011b and held thereafter. Then, the DMF is discharged, and the deprotected solid resin remaining on the upper surface 1051 of the first filter 105 is cleaned. This cleaning process is performed multiple times.

[0211] Then, the second valve 103b is opened, and HBTU, DIPEA, N-terminal bonding amino acids protected with Fmoc groups, and DMF as a solvent are supplied into the container 301 through the second opening 1011b. HBTU, DIPEA, and bonding amino acids are dissolved in the DMF. Microwaves are irradiated by the irradiation device 302, causing the N-terminal protected amino acids and the deprotected amino acids already bonded to the solid resin to bond through a condensation reaction. Similarly, the container 301 is provided with a first filter 105 and a second filter 107. Therefore, the area between the first filter 105 and the second filter 107 contains the bonding solution containing HBTU, DIPEA, and bonding amino acids dissolved in DMF, as well as the deprotected solid resin. The area below the first filter 105 and the area above the second filter 107 only contains the bonding solution. However, the bonding solution moves through the first filter 105, the first reflective member 106, the second filter 107, and the second reflective member 108. The microwaves emitted by the irradiation device 302 are similar to those described above, making it difficult for the microwaves to irradiate the areas below the first filter 105 where there is no solid resin and the areas above the second filter 107 where there is no solid resin, i.e., areas that do not require the treatment of bonding amino acids.

[0212] After the amino acid bonding is completed, if the first valve 103a is opened, the bonding solution will be discharged from the container 301, and the solid resin of the amino acid with the N-terminus of the new bond protected will not be discharged but will remain on the upper surface 1051 of the first filter 105.

[0213] The cleaning process using DMF was then repeated multiple times in the same manner as described above.

[0214] This allows peptides bonded to the solid resin to be obtained on the upper surface 1051 of the first filter 105. Subsequent processing is the same as in Embodiment 1 described above, and therefore will not be described here.

[0215] According to the present embodiment, microwaves are irradiated into the microwave-reflective container 301, thereby enabling the container 301 to be enlarged and increasing the processing capacity of peptides and the like synthesized by solid phase.

[0216] Furthermore, according to this embodiment, a first filter 105 is stacked on top of a first reflective member 106, and a second reflective member 108 is stacked on top of a second filter 107. In this way, the first reflective member 106 reflects microwaves that pass through the first filter 105, and the second reflective member 108 reflects microwaves that pass through the second filter 107. This makes it difficult for microwaves to irradiate the contents without solid resin below the first filter 105 and above the second filter 107, and the microwaves can be used efficiently for solid-phase synthesis processing.

[0217] Furthermore, according to this embodiment, a first filter 105 is superimposed on the first reflective member 106, thereby reinforcing the first filter 105 through the first reflective member 106, thereby reducing, for example, the required strength or hardness of the first filter 105, and increasing the selectivity of the first filter 105.

[0218] Furthermore, according to this embodiment, the second reflective member 108 is overlapped and disposed on the second filter 107, thereby reinforcing the second filter 107 through the second reflective member 108, thereby reducing, for example, the limitations required for the strength or hardness of the second filter 107, and increasing the selectivity of the second filter 107.

[0219] (First variation)

[0220] Figure 6 (a)- Figure 6 (c) is a cross-sectional view illustrating a first modified example of the processing apparatus of this embodiment, which is equivalent to Figure 5 (b) is a cross-sectional view.

[0221] The above embodiment describes the case where the first filter 105 is overlapped on the first reflective member 106, that is, on the second end 1015b side of the first reflective member 106. However, as long as the first reflective member 106 is disposed between the first filter 105 and the first end 1015a, preferably between the first filter 105 and the first opening 1011a, the first filter 105 may not be directly overlapped on the first reflective member 106.

[0222] For example Figure 6 As shown in (a), the first filter 105 and the first reflective member 106 can be configured such that they do not directly overlap. This configuration of the first filter 105 and the first reflective member 106 is equivalent to the variation used in embodiment 1 described above. Figure 3 (a) A variation of the example is described, with the detailed description of the variation omitted.

[0223] Furthermore, instead of the first filter 105 and the first end portion 1015a, the first reflective member 106 is preferably disposed between the first filter 105 and the first opening 1011a, such as... Figure 6 As shown in (b), a first reflective member 106 can be disposed between the first filter 105 and the microwave emission position. This arrangement of the first filter 105 and the first reflective member 106 is used in a variation of embodiment 1 described above. Figure 3 (b) A modified example is described, with detailed description of the modified example omitted. In addition, in this case, the first filter 105 may be made of a material other than a microwave-permeable material.

[0224] Furthermore, as an example of the case where the first reflective member 106 is disposed between the first filter 105 and the microwave emission position, such as... Figure 6 As shown in (c), the first reflective member 106 can be disposed overlapping on the upper surface side of the first filter 105. This arrangement of the first filter 105 and the first reflective member 106 is used in a variation of embodiment 1 described above. Figure 3 (c) The variation of the example is described, and the detailed description of the variation is omitted.

[0225] (Second variation)

[0226] Figure 7 (a)- Figure 7 (c) is a cross-sectional view of a second modified example of the processing apparatus used to illustrate this embodiment, which is equivalent to Figure 5 (b) is a cross-sectional view.

[0227] The above describes a modified example of the first filter 105 and the first reflective member 106. However, as will be explained below, the second filter 107 and the second reflective member 108 may also be modified in the same way as the first modified example described above.

[0228] For example, in the above embodiment, the second reflective member 108 is superimposed on the second filter 107. However, as long as the second reflective member 108 is disposed between the second filter 107 and the second end 1015b, preferably between the second filter 107 and the second opening 1011b, the second reflective member 108 may not be directly superimposed on the second filter 107.

[0229] For example Figure 7 As shown in (a), the second filter 107 and the second reflective member 108 can be arranged in a manner that does not directly overlap. Even in this case, the microwaves passing through the second filter 107 are reflected by the second reflective member 108 positioned above the second filter 107 to prevent microwaves from irradiating the area above the second reflective member 108, and the microwaves can still be efficiently utilized for solid-phase synthesis. However, at this time, the microwaves irradiate the contents that do not contain solid-phase resin between the second filter 107 and the second reflective member 108, so compared to the case where the second filter 107 is overlapped on the second reflective member 108, the microwaves cannot be utilized efficiently. Furthermore, the second filter 107 cannot be reinforced by the second reflective member 108 at this time, so the second filter 107 must be a second filter 107 that has sufficient strength even without reinforcement, or a reinforcing member (such as a reinforcing frame) made of microwave-permeable material must be installed on the second filter 107, which limits the materials or structures that can be used as the second filter 107, as the installation of reinforcing members will complicate the structure and increase costs.

[0230] also, Figure 5 and Figure 7 The processing device 3 described in (a) may be an example in which a second reflective member 108 is provided on the side of the second filter 107 closer to the second end 1015b. In particular, it may be an example in which the second filter 107 is disposed between the microwave emitting position and the second end 1015b, and a second reflective member 108 is provided on the side of the second filter 107 closer to the second end 1015b.

[0231] Furthermore, instead of the second reflective member 108, it is disposed between the second filter 107 and the second end portion 1015b, preferably between the second filter 107 and the second opening 1011b, such as... Figure 7As shown in (b), a second reflective member 108 can be disposed between the second filter 107 and the emission position of the emitted microwaves. In this case, the second reflective member 108 reflects the microwaves, thereby preventing the microwaves from easily irradiating the area above the second filter 107, that is, the area between the second filter 107 and the second end 1015b, and the microwaves can be utilized efficiently. In this case, the second filter 107 can prevent the solid resin from moving to the area above the second end 1015b of the second filter 107, so the second reflective member 108 can also be a member that allows the solid resin to pass through. For example, the second reflective member 108 can be a member with multiple holes, the multiple holes being of a shape and size that allows the solid resin to pass through. In addition, in this case, it is not easy to irradiate the contents containing the solid resin between the second reflective member 108 and the second filter 107 with microwaves, so if the distance between the second reflective member 108 and the second filter 107 becomes wider, the processing efficiency of solid-phase synthesis may be reduced. Also, in this case, the second filter 107 can also be made of a material other than a microwave-permeable material.

[0232] Furthermore, as an example of arranging the second reflective member 108 between the second filter 107 and the microwave emission position, such as... Figure 7 As shown in (c), the second reflective member 108 can be overlapped on the first end 1015a side of the second filter 107. That is, the second filter 107 can be overlapped on the second reflective member 108. In this case, the distance between the second reflective member 108 and the second filter 107 can be eliminated, preventing a decrease in the processing efficiency of solid-phase synthesis as described above. Simultaneously, the second filter 107 can be positioned on the second reflective member 108 and reinforced by the second reflective member 108. As described in the above embodiment or... Figure 7 (c) The second filter 107 and the second reflective member 108 are arranged in an overlapping manner, thereby the second reflective member 108 can reinforce the second filter 107 and increase the selectivity of the second filter 107.

[0233] Figure 7 (b) and Figure 7 The processing device 3 described in (c) is an example in which the second filter 107 is disposed between the second end 1015b and the microwave emission position, and a second reflective member 108 is provided between the second filter 107 and the microwave emission position.

[0234] Furthermore, in the above embodiment 3, the following describes the situation where the first filter 105 is disposed between the first end 1015a of the container 301 and the emission position where the irradiation device 302 emits microwaves, the first reflective member 106 is disposed between the first end 1015a of the container 301 and the emission position, the second filter 107 is disposed between the second end 1015b of the container 301 and the emission position, and the second reflective member 108 is disposed between the second end 1015b of the container 301 and the emission position. However, in the processing apparatus 3, it is sufficient that the first filter 105 is arranged in a partition container 301, the first reflective member 106 is arranged in a partition container 301 on the side closer to the first end 1015a than the ejection position, the ejection position is arranged between the first end 1015a and the second end 1015b, the second filter 107 is arranged in a partition container 301 on the side closer to the second end than the first filter 105 and the first reflective member 106, and the second reflective member 108 is arranged in a partition container 301 on the side closer to the second end 1015b than the first filter 105 and the ejection position. However, it is preferable that the first filter 105 and the first reflective member 106 are arranged away from the first opening 1011a, and the second filter 107 and the second reflective member 108 are arranged away from the second opening 1011b.

[0235] For example, in processing apparatus 3, a first filter 105 may be disposed between the first end 1015a and the second end 1015b of container 301 in a manner separating container 301; a first reflective member 106 may be disposed between the first end 1015a and the emission position; a second filter 107 may be disposed between the first filter 105 and the first reflective member 106 and the second end 1015b in a manner separating container 301; and a second reflective member 108 may be disposed between the first filter 105 and the emission position and the second end 1015b in a manner separating container 301. Even in this case, the microwaves irradiated into container 301 may not easily irradiate between the first reflective member 105 and the first end 1015a, or between the second reflective member 107 and the second end 1015b, yet the same effect as the above embodiment can still be achieved. In this case, solid materials such as solid resin that are the objects of separation only need to be supplied, for example, between the first filter 105 and the second filter 107.

[0236] Furthermore, the so-called second filter 107 being disposed between the first filter 105 and the first reflective member 106 and the second end 1015b can be interpreted, for example, as the second filter 107 being disposed between the first filter 105 and the second end 1015b, and between the first reflective member 106 and the second end 1015b. The so-called "between the first filter 105 and the first reflective member 106 and the second end 1015b" can, for example, be the space between both the first filter 105 and the first reflective member 106 and the second end 1015b. Furthermore, the situations described above, such as the second reflective member 108 being disposed between the first filter 105 and the emission position and the second end 1015b, the second filter 107 being disposed between the first filter 105 and the first reflective member 106 and the second end 1015b, the second reflective member 108 being disposed between the first filter 105 and the emission position and the second end 1015b, the second filter 107 being disposed between the first filter 105 and the first reflective member 106 and the second end 1015b, and the second reflective member 108 being disposed between the first filter 105 and the emission position and the second end 1015b, are explained in the same way as described above.

[0237] For example, such as Figure 6 (a) When the first reflective member 106 is disposed on the side closer to the first end 1015a than the first filter 105 and the first filter 105 is separately disposed from the first reflective member 106, the irradiation device 302 can irradiate microwaves into the container 301 from the position between the first filter 105 and the first reflective member 106. That is, the microwave emission position can be the position between the first filter 105 and the first reflective member 106 of the container 301. Solid materials such as solid resin, which are the objects of separation, are supplied, for example, between the first filter 105 and the second filter 107. In this configuration, as described above, the first filter 105 is disposed between the first end 1015a and the second end 1015b of the container 301, the second filter 107 is disposed between the first filter 105 and the first reflective member 106 and the second end 1015b, and the second reflective member 108 is disposed between the first filter 105 and the emission position and the second end 1015b. The position between the first filter 105 and the first reflective member 106 of the container 301 refers to, for example, the position of the container 301 in the height direction between the first filter 105 and the first reflective member 106. For example, as long as an irradiation opening 1013 is provided at the position between the first filter 105 and the first reflective member 106 of the container 301, the irradiation device 302 can irradiate microwaves into the container 301 through the irradiation opening 1013.

[0238] For example, such as Figure 7(a) When the second reflective member 108 is disposed on the side closer to the second end 1015b than the second filter 107 and the second filter 107 and the second reflective member 108 are separately disposed, the irradiation device 302 can irradiate microwaves into the container 101 from the position between the second filter 107 and the second reflective member 108 of the container 301. That is, the microwave emission position can be the position between the second filter 107 and the second reflective member 108 of the container 301. Solid materials such as solid resin, which are the objects of separation, are supplied, for example, between the first filter 105 and the second filter 107. In this configuration, as described above, the first filter 105 is disposed between the first end 1015a and the second end 1015b of the container 301, the second filter 107 is disposed between the first filter 105 and the first reflective member 106 and the second end 1015b, and the second reflective member 108 is disposed between the first filter 105 and the emission position and the second end 1015b. Here, the position between the second filter 107 and the second reflective member 108 of the container 301 refers to, for example, the position of the container 301 in the height direction between the second filter 107 and the second reflective member 108. For example, as long as an irradiation opening 1013 is provided at the position between the second filter 107 and the second reflective member 108 of the container 301, and the irradiation device 302 irradiates microwaves into the container 301 through the irradiation opening 1013, it is sufficient.

[0239] Furthermore, the second filter 107 and the second reflective member 108 of the processing apparatus 3 in the above embodiment 3 and its first modification can be used in the second modification of the above embodiment 3. Figure 7 (a)- Figure 7 (c) Either the second filter 107 and the second reflective member 108 described separately. Furthermore, as the first filter 105 and the first reflective member 106 of the above embodiment 3 and its second variation, the first variation of embodiment 3 can be used... Figure 6 (a)- Figure 6 (c) Any combination of the first filter 105 and the first reflective member 106 described separately. For example, the processing device 3 may be any combination of the first filter 105 and the first reflective member 106 described in the above embodiment 3 and its first variation, or any combination of the second filter 107 and the second reflective member 108 described in the above embodiment 3 and its second variation.

[0240] (Implementation Form 4)

[0241] The processing apparatus of this embodiment is the same as that described in embodiment 3 above, except that a microwave-reflecting filter is used to replace the first filter and the first reflective member, and a second filter and the second reflective member, respectively.

[0242] Figure 8 This is a perspective view showing an example of the processing apparatus in this embodiment. Figure 8 (a)) and its cross-sectional view of line VIIIb-VIIIb ( Figure 8 (b)). But Figure 8 (b) Omits the cross-section of the valve, etc.

[0243] The processing apparatus 4 of this embodiment includes a container 301, an irradiation device 302, a first valve 103a, a second valve 103b, a first reflective filter 205, and a second reflective filter 206. The container 301 includes a first opening 1011a, a second opening 1011b, a third opening 1011c, and an irradiation opening 1013. Furthermore, the configuration other than the first reflective filter 205 and the second reflective filter 206 is the same as that of Embodiment 3 described above, so detailed descriptions are omitted here.

[0244] The first reflective filter 205 is a filter formed by integrating the first filter 105 and the first reflective member 106. It is a filter that separates the solid resin from the contents of the container 301 and reflects the microwaves irradiated into the container by the irradiation device 302. The first reflective filter 205 is described in Embodiment 2 above, so a detailed description is omitted here.

[0245] The configuration of the first reflective filter 205 is the same as that of embodiment 2 described above, except that the emission position is the emission position of the irradiation device 302. Therefore, detailed description is omitted here.

[0246] The second reflective filter 206 is a filter formed by integrating the second filter 107 and the second reflective member 108. It is a filter that separates solid resin from the contents of the container 301 and reflects microwaves irradiated into the container by the irradiation device 302. The second reflective filter 206 can utilize the same components as the first reflective filter 205, so detailed descriptions are omitted here. The first reflective filter 205 and the second reflective filter 206 can use the same components or different components.

[0247] The second reflective filter 206 is an integrated component of the second filter 107 and the second reflective member 108, so it only needs to be disposed at a position where both the second filter 107 and the second reflective member 108 can be disposed. Therefore, the second reflective filter 206 is disposed similarly to the second reflective member 108 in the above embodiment, between the second end 1015b and the emission position where the irradiation device 302 emits microwaves. Furthermore, the second opening 1011b only needs to be provided on the side above the second reflective filter 206, that is, on the side of the second end 1015b. In addition, reinforcing frames or the like can be installed in the first reflective filter 205 and the second reflective filter 206.

[0248] Even in this embodiment, the container 301 can be enlarged, similar to the embodiments described above, to increase the processing capacity of peptides and the like synthesized via solid-phase synthesis. Furthermore, a first reflective filter 205 is provided, thereby allowing the solid resin to be separated from the contents. Also, the regions below the first reflective filter 205 and above the second reflective filter 206 within the container 301 are designed to prevent the solid resin between the first and second reflective filters 205 from moving, thus eliminating the presence of solid resin in these areas. Microwaves irradiated by the irradiation device 302 are reflected by the first and second reflective filters 205 and 206 to the region between them, thereby preventing microwaves from easily irradiating the resin-free contents below the first and second reflective filters 205 and above the second reflective filters 206, allowing for efficient use of microwaves in the solid-phase synthesis process.

[0249] In addition, such as Figure 5 and Figure 7 (a)- Figure 7 As shown in (c), the first reflective filter 205 of embodiment 4 can be used to replace the first filter 105 and the first reflective member 106 of the processing device 3 described in embodiment 3 and its second modification, respectively. Figure 5 and Figure 6 (a)- Figure 6 (c) As shown, the second reflective filter 206 of embodiment 4 can be used to replace the second filter 107 and the second reflective member 108 of the processing device 3 described in embodiment 3 and its first modification. For example, the processing device only needs to have the combination of the first filter 105 and the first reflective member 106 described in embodiment 3 and its first modification and any one of the first reflective filter 205, or the combination of the second filter 107 and the second reflective member 108 described in embodiment 3 and its second modification and any one of the second reflective filter 206.

[0250] Furthermore, while embodiments 1-4 describe the processing device having an irradiation device 102 or an irradiation device 302, the processing device may or may not have an irradiation device 102 or an irradiation device 302. For example, the processing device may be a portion of the processing device 1 without the irradiation device 102 or the irradiation device 302, and the irradiation device 102 or the irradiation device 302 may be a device different from the processing device. In this case, when using the processing device to perform microwave irradiation processing, it is sufficient, for example, to prepare a separate irradiation device 102 or an irradiation device 302 that is different from the processing device and install it in the processing device.

[0251] Furthermore, in embodiments 1-4 described above, an example is given where the container 101 has an illumination opening 1013 and microwaves are irradiated through this illumination opening 1013. However, the container 101 only needs to have an emitting part (not shown) that irradiates microwaves into the container 101, as long as microwaves can be irradiated into the container 101 through this emitting part. The microwave emitting part is the portion that emits microwaves that irradiate into the container 101. The emitting part can have any structure or shape, as long as it can, for example, radiate (or introduce) the microwaves irradiated by the illumination device 102 into the container 101. For example, the illumination opening 1013 in the above embodiments can be the emitting part. Furthermore, the position of this emitting part can be the position where the microwaves are emitted. Furthermore, the positional relationship of the first filter 105 or the first reflective member 106, the second filter 107, the second reflective member 108, etc., relative to the emission position can be interpreted as the positional relationship relative to the illumination opening 1013 or the emitting part.

[0252] (Implementation Form 5)

[0253] Figure 9 This is a perspective view showing an example of a tubular column in this embodiment. Figure 9 (a) and its cross-sectional view of the IXb-IXb line ( Figure 9 (b)).

[0254] Figure 10 This is a perspective view of the first filter of the tubular column in this embodiment, viewed from an oblique angle. Figure 10 (a) A three-dimensional view of the first reflecting component viewed from an oblique angle ( Figure 10 (b) A perspective view of the first reflective member and the first filter disposed on the reflective member, viewed from an oblique angle. Figure 10 (c) and a perspective view of the reflective element and the filter disposed on the reflective element from an oblique downward view. Figure 10 (d)).

[0255] The tubular column 5 includes a cylindrical member 501, a first filter 502, a first reflective member 503, a second filter 504, and a second reflective member 505. The cylindrical member 501 has a first end 5011a, a second end 5011b, an inlet 5013, and a microwave permeable member 5014.

[0256] In this embodiment, column 5 is used as an example of a solid-phase synthesis column for synthesizing peptides already bonded to a solid-phase resin. However, as described later, column 5 can be used for processes other than peptide solid-phase synthesis.

[0257] The tubing 5 in this embodiment is used to perform one or more of the processes constituting solid-phase synthesis as described in Embodiment 1 above. The tubing 5 is, for example, installed in and used in a processing apparatus (not shown) for performing solid-phase synthesis. The tubing 5 in this embodiment can be used, for example, in one or more of the processes described in Embodiment 1 above.

[0258] Cylindrical member 501 is a member having a cylindrical shape. Cylindrical member 501 is a hollow member. Cylindrical member 501 is a cylindrical shape with a circular cross-section perpendicular to the axial direction. The length of cylindrical member 501 in the axial direction is not limited. Furthermore, the diameter of the cross-section perpendicular to the axial direction of cylindrical member 501 is not limited. Also, the cross-sectional shape of cylindrical member 501 can be a shape other than a perfect circle, for example, a polygon or an ellipse. Preferably, the cross-sectional shape of the inner surface of cylindrical member 501 is the same shape and size at any position in the axial direction. However, the cross-sectional shape of the inner surface of cylindrical member 501 can also be different in shape or size at different positions in the axial direction, or partially different in shape or size in the axial direction. Cylindrical member 501 can be a cylindrical container.

[0259] The cylindrical member 501 has a first end 5011a and a second end 5011b. The first end 5011a and the second end 5011b are portions at both ends of the cylindrical member 501 in the axial direction. Both the first end 5011a and the second end 5011b are open. The inner diameter of the open portion is the same as the inner diameter of the other portions of the cylindrical member 501. However, the inner diameter of the open portion may be slightly wider or slightly narrower than the other portions. Here, the case where the cylindrical member 501 is used with the axial direction as the vertical direction is described. Also, the case where the cylindrical member 501 is used with the first end 5011a as the lower end and the second end 5011b as the upper end is described. Furthermore, the open portion of the first end 5011a will be referred to as the first opening portion 5012a, and the open portion of the second end 5011b will be referred to as the second opening portion 5012b.

[0260] The cylindrical member 501 is made of a material with microwave reflectivity. For example, a conductive material or a metal such as stainless steel is used. The cylindrical member 501 is preferably made of a material with excellent corrosion resistance. For example, stainless steel is preferred. The thickness of the outer wall of the cylindrical member 501 is not fixed. The inner wall of the cylindrical member 501 may be coated with a material such as polytetrafluoroethylene (PTFE) or glass, which has high microwave permeability and excellent corrosion resistance. For example, the cylindrical member 501 may be a dual-structure container with an inner wall made of the aforementioned material with high microwave permeability and excellent corrosion resistance, and an outer wall made of a microwave reflective material such as stainless steel.

[0261] The cylindrical member 501 has an inlet 5013 on its side for introducing microwaves from the outside of the cylindrical member 501 into its interior. The side of the cylindrical member 501 can be the outer periphery or sidewall of the cylindrical member 501. The interior of the cylindrical member 501 can be the inner side or the inside of the cylindrical member 501. Introducing microwaves can mean irradiating the interior of the cylindrical member 501 with microwaves. The inlet 5013 can be, for example, the part that emits microwaves irradiated into the cylindrical member 501, that is, the microwave emitting part. The inlet 5013 is an opening blocked by a plate-shaped microwave permeable member 5014. The shape of the inlet 5013 can be, for example, rectangular, circular, or slit-shaped, and its shape and size are not limited. Furthermore, this indicates the case where only one inlet 5013 is provided, but the number of inlet 5013s can also be multiple. The inlet 5013 is provided near the center in the axial direction of the cylindrical member 501. However, the location of the inlet 5013 on the side of the cylindrical member 501 is not restricted.

[0262] The microwave-permeable member 5014 is a member made of a microwave-permeable material. The microwave-permeable member 5014 is preferably made of a material with excellent corrosion resistance. Here, we will describe the case where the microwave-permeable member 5014 is a plate-shaped member made of PTFE. Alternatively, the microwave-permeable member 5014 may not be a plate-shaped member. The inlet 5013 is blocked with a microwave-permeable material, so microwaves can be introduced into the interior of the cylindrical member 501 from the outside through the inlet 5013, and the contents of the cylindrical member 501 can be prevented from being discharged to the outside through the inlet 5013.

[0263] The area around the inlet 5013 may include a waveguide for introducing microwaves into the cylindrical member 501 or a connector (not shown) for mounting an antenna. Alternatively, instead of blocking the inlet 5013 with a microwave-permeable member 5014, it can be blocked with an antenna or the like, which is used to introduce microwaves into the cylindrical member 501.

[0264] In cases where the opening (not shown) on the cylindrical member 501 side of a waveguide (not shown) installed in a manner that blocks the inlet 5013 is blocked by a member of microwave-permeable material, the inlet 5013 may not be blocked by a member of microwave-permeable material. Furthermore, in cases where an antenna irradiating microwaves installed at the inlet 5013 can block the opening of the inlet 5013, the inlet 5013 may be an opening that is not blocked by a member of microwave-permeable material.

[0265] Furthermore, the method of introducing microwaves into the cylindrical member 501 through the inlet 5013 is not limited. For example, a waveguide (not shown) or a coaxial cable (not shown) with an antenna, which propagates microwaves irradiated by a microwave irradiation device (not shown), can be connected to the inlet 5013, and microwaves can be irradiated through the waveguide. Alternatively, the entire column 5 or at least the side portion of the cylindrical member 501 can be disposed on a waveguide or the like that that propagates microwaves, thereby allowing microwaves to be introduced into the cylindrical member 501 through the inlet 5013.

[0266] Furthermore, the inlet 5013 is preferably located on the side of the cylindrical member 501, in a region closer to the second end 5011b than the first filter 502 and the first reflector 503, and in a region closer to the first end 5011a than the second filter 504 and the second reflector 505.

[0267] The cylindrical member 501 is, for example, a container in which one or more processes constituting a solid-phase synthesis process are carried out internally. Similar to the embodiments described above, substances, intermediate products, or solvents used in solid-phase synthesis are supplied and maintained inside the cylindrical member 501. For example, the substances or intermediate products described in the cylindrical member 501 are maintained together with a suitable solvent. Furthermore, the supply and discharge of contents to the cylindrical member 501 can be performed continuously, and the process can be continuously carried out while the contents are flowing within the cylindrical member 501.

[0268] In addition, similar to the embodiment described above, a stirring device (not shown) for stirring contents may be provided inside the cylindrical member 501.

[0269] Although the outer periphery of the cylindrical component 501 is not shown in the figure, it may be equipped with a warm water jacket or cold water jacket, heater, etc., to adjust the temperature of the cylindrical component 501.

[0270] The shape and size of the cylindrical member 501 are preferably set in such a way that the microwave mode within the cylindrical member 501 is multimode. For example, the shape and size of the cylindrical member 501 are preferably such that microwaves introduced through the inlet 5013 irradiate the cylindrical member 501 in multimode. For example, the so-called multimode microwave irradiation is, for example, a mode in which microwave standing waves are not generated within the cylindrical member 501.

[0271] The first filter 502 is, for example, a solid resin used in solid-phase synthesis that is separated from the contents within the cylindrical member 501. The first filter 502 has, for example, a plurality of pores for separating the solid resin used in solid-phase synthesis from the contents within the cylindrical member 501. The pores are, for example, pores that connect from the surface 5021 to the back surface. The surface 5021 of the first filter 502 is, in this case, the surface on the side of the second end 5011b of the first filter 502. For example, the plurality of pores of the first filter 502 are pores with a size smaller than the particle size of the solid resin contained in the contents. In this way, only the solid resin in the contents within the cylindrical member 501 is filtered and separated and remains on the surface 5021 of the first filter 502, while activators or deprotectants dissolved in the solvent, or amino acids dissolved or suspended in the solvent, pass through the pores of the first filter 502 together with the solvent. Furthermore, the first filter 502 may utilize the same components as the first filter 102 described in the above embodiment, so detailed descriptions are omitted here. In this embodiment, as an example, a sheet-like first filter 502 made of porous PTFE material is used, similar to the embodiments described above.

[0272] The first filter 502 is configured to block the first end 5011a side of the cylindrical member 501. "Configuring the first filter 502 to block the first end 5011a side" means that the first filter 502 is configured to block the first opening 5012a or its vicinity at the first end 5011a of the cylindrical member 501. The "first end 5011a side" can be, for example, the first end 5011a itself or its vicinity. "Blocking the cylindrical member 501 with the first filter 502" means, for example, that the contents of the cylindrical member 501 do not pass through the first filter 502 and do not move from the first end 5011a to the outside. By blocking the cylindrical member 501 with the first filter 502, for example, the contents of the cylindrical member 501 that can pass through the first filter 502 will pass through the first opening 5012a at the first end 5011a side of the first filter 502 and can move between the inside and outside of the cylindrical member 501. Furthermore, the contents here refer to the contents that are included within the cylindrical member 501, such as the contents supplied to or added to the cylindrical member 501 at the moment prior to this. The same applies below.

[0273] The first filter 502 can be disposed at a position relative to the first end 5011a on the side of the second end 5011b in the axial direction. For example, it can be disposed at a distance of 3 cm or less from the first end 5011a, preferably 1 cm or less, on the side of the second end 5011b closer to the first end 5011a. Here, the first filter 502 is disposed in an overlapping manner on the side of the second end 5011b of the first reflective member 503, which will be described later. Therefore, the first filter 502 is disposed at a position closer to the second end 5011b than the first end 5011a by the thickness of the first reflective member 503.

[0274] Furthermore, the first filter 502 can be configured in such a way that it can block the first end 5011a of the cylindrical member 501. The so-called configuration in such a way that it can block the first end 5011a means, for example, that the first filter 502 is configured at the first opening 5012a of the first end 5011a, and the first opening 5012a of the first end 5011a is blocked by the first filter 502.

[0275] The first reflective member 503 is a flat plate-shaped member made of microwave reflective material and having a plurality of holes 503a that reflect microwaves introduced into the cylindrical member 501. These holes 503a allow the contents of the cylindrical member 501, which have passed through at least the first filter 502, to pass through. The holes 503a are connected, for example, from a surface 5031 on the second end 5011b side of the first reflective member 503 to a back surface 5032. The diameter of each hole 503a is set to allow the contents that have passed through at least the first filter 502 to pass through and reflect microwaves introduced into the inlet 5013. The size of each hole 503a is only required to be, for example, a size through which at least the contents of the cylindrical member 501 that have passed through the first filter 502 can pass, such as a size through which the portion of the contents of the cylindrical member 501 after removing the solid resin separated by the first filter 502 can pass, or a size through which contents that also contain solid resin can pass. For example, the first reflective member 503 may be a mesh made of microwave reflective material such as stainless steel, which has multiple openings of a size that allow the contents of the cylindrical member 501 of at least the first filter 502 to pass through. These openings may correspond to the holes in the first reflective member 503. Furthermore, the first reflective member 503 may utilize the same component as the first reflective member 106 described in the above embodiment, and therefore a detailed description is omitted here.

[0276] In this embodiment, one example of the first reflective member 503 is a perforated stainless steel sheet with a plurality of circular holes 503a. These holes 503a have a diameter larger than that of the solid resin and are sized to reflect microwaves introduced into the inlet 5013. Thus, the first reflective member 503 allows the contents of the first filter 502 to pass through and reflects microwaves introduced into the inlet 5013.

[0277] also, Figure 9 , Figure 10 (b) Figure 10 (d) and the like are for illustrative purposes. The relationship between the dimensions of the plurality of holes 503a provided on the first reflective member 503 and the dimensions of the first reflective member 503, or the number of the plurality of holes 503a, are for illustrative purposes and are not necessarily the same as the actual first reflective member 503. Furthermore, the first reflective member 503 may not be a flat plate.

[0278] The first reflective member 503 can be made of any material and can have any shape or structure other than those described above, as long as it is a member that allows the contents of the cylindrical member 501, at least the first filter 502, to pass through and reflects microwaves introduced into the inlet 5013. In this way, the first reflective member 503 reflects microwaves introduced into the cylindrical member 501 through the inlet 5013 and allows the contents of the cylindrical member 501, at least the first filter 502, to pass through. For example, it is acceptable as long as the length of the widest portion of the plurality of openings is less than half the wavelength of the microwaves introduced into the inlet 5013, and the narrowest portion of its opening is wider than the contents passing through the first filter 502. The planar shape of the plurality of openings of the mesh is not restricted.

[0279] Furthermore, when the first filter 502, which is superimposed on the first reflective member 503, is reinforced and supported, the material of the first reflective member 503 is preferably a high-strength material such as metal.

[0280] The first reflective member 503 is configured to block the first end portion 5011a of the cylindrical member 501. This configuration means, for example, that the first reflective member 503 is positioned at the first opening 5012a of the first end portion 5011a of the cylindrical member 501, and the first opening 5012a of the first end portion 5011a is blocked by the first reflective member 503. For example, the configuration of the first reflective member 503 blocking the first end portion 5011a of the cylindrical member 501 can be such that microwaves introduced into the cylindrical member 501 do not pass through the first opening 5012a of the first end portion 5011a to the outside, by placing the first reflective member 503 in the cylindrical member 501. Furthermore, the configuration of the first reflective member 503 blocking the cylindrical member 501 can be such that the contents of the cylindrical member 501 do not move from the first end portion 5011a to the outside without passing through the first reflective member 503. The first reflective member 503 blocks the cylindrical member 501, thereby allowing, for example, the contents of the cylindrical member 501 that can pass through the first reflective member 503 to pass through the first opening 5012a on the first end 5011a side and move between the inside and outside of the cylindrical member 501.

[0281] A first filter 502 is disposed overlapping the second end 5011b side of the first reflective member 503. The first filter 502 is disposed on the surface 5031 of the second end 5011b side of the first reflective member 503.

[0282] Furthermore, when the first filter 502 is disposed on the underside of the first reflective member 503 in an overlapping manner, the first filter 502 is preferably fixed to the first end 5011a side of the first reflective member 503.

[0283] Furthermore, the first reflective member 503 can be positioned on the second end 5011b side relative to the first end 5011a in the axial direction. For example, the first reflective member 503 can be positioned on the second end 5011b side relative to the first end 5011a at a distance of 3 cm or less, preferably 1 cm or less. That is, the first reflective member 503 only needs to be positioned to block the first end 5011a side of the cylindrical member 501. Positioning the first reflective member 503 to block the first end 5011a side means positioning it to block the first opening 5012a or its vicinity at the first end 5011a. The first end 5011a side can be, for example, the first opening 5012a or its vicinity at the first end 5011a.

[0284] Furthermore, the planar shape of the first reflective member 503 may be, for example, simply the shape and size of the first opening 5012a that can block the first end 5011a.

[0285] The second filter 504 is, for example, a component that separates the solid resin used in solid-phase synthesis from the contents within the cylindrical member 501. The second filter 504 can, for example, use the same component as the first filter 502 described above, so a detailed description is omitted here. Furthermore, the second filter 504 can be the same as or different from the first filter 502. For example, if the material of the second filter 504 is a microwave-permeable material, it can be the same material as the first filter 502 or a different material. Also, if the plurality of holes in the second filter 504 are of the size or shape required to separate the solid resin used in solid-phase synthesis from the contents within the cylindrical member 501, they can be holes of the same size or shape as the first filter 502 or holes of a different size or shape.

[0286] The second filter 504 is configured to block the second end 5011b side of the cylindrical member 501. This means that the second filter 504 is configured to block the second opening 5012b or its vicinity at the second end 5011b of the cylindrical member 501. The second end 5011b side can be, for example, the second end 5011b itself or its vicinity. Blocking the cylindrical member 501 with the second filter 504 means, for example, that the contents within the cylindrical member 501 do not move from the second end 5011b to the outside without passing through the second filter 504. By blocking the cylindrical member 501 with the second filter 504, for example, the contents within the cylindrical member 501 that can pass through the second filter 504 can pass through the second opening 5012b at the second end 5011b side of the second filter 504 and can move between the inside and outside of the cylindrical member 501. Furthermore, the contents here refer to the concept of contents supplied within the cylindrical member 501.

[0287] The second filter 504 can be configured at a position in the axial direction relative to the second end 5011b on the side of the first end 5011a. For example, it can be configured at a distance of 3 cm or less, preferably 1 cm or less, from the second end 5011b on the side of the first end 5011a. Here, the second filter 504 is configured in an overlapping manner on the side of the first end 5011a of the second reflective member 505 (described later). Therefore, the second filter 504 is configured at a position closer to the first end 5011a than the second end 5011b by the thickness of the second reflective member 505.

[0288] Furthermore, the second filter 504 can be configured to block the second end 5011b of the cylindrical member 501. "Configured to block the second end 5011b" means, for example, that the second filter 504 is configured at the second opening 5012b of the second end 5011b, and the second opening 5012b of the second end 5011b is blocked by the second filter 504.

[0289] The second reflective member 505 is a member that allows the contents of at least the second filter 504 (e.g., the contents after the solid resin has been removed by the second filter 504) to pass through and reflects microwaves. The second reflective member 505 may be the same as the first reflective member 503 described above, so a detailed description is omitted. Here, we will illustrate an example where the second reflective member 505 is a flat plate-shaped member made of microwave-reflective material and has multiple holes. These holes reflect microwaves introduced into the cylindrical member 501 and allow the contents of the cylindrical member 501 (e.g., the contents after the solid resin has been removed) to pass through the second filter 504.

[0290] Furthermore, the second reflective member 505 may be the same as or different from the first reflective member 503. For example, as long as the material of the second reflective member 505 is a microwave-reflective material, it may be the same material as the first reflective member 503 or a different material. Also, for example, as long as the plurality of holes of the second reflective member 505 are of a size or shape that allows the contents of the solid resin used for solid-phase synthesis to pass through, they may be holes of the same size or shape as the holes 503a of the first reflective member 503, or they may be holes of a different size or shape.

[0291] The second reflective member 505 is configured to block the second end portion 5011b of the cylindrical member 501. This configuration means, for example, that the second reflective member 505 is positioned at the second opening 5012b of the second end portion 5011b of the cylindrical member 501, thereby blocking the second opening 5012b of the second end portion 5011b. For example, this configuration could be such that microwaves introduced into the cylindrical member 501 do not pass through the second opening 5012b of the second end portion 5011b to the outside, by placing the second reflective member 505 on the cylindrical member 501. Furthermore, this configuration could also be such that the contents of the cylindrical member 501 do not move from the second end portion 5011b to the outside without passing through the second reflective member 505. The second reflective member 505 blocks the cylindrical member 501, thereby allowing contents within the cylindrical member 501 to pass through the second opening 5012b on the second end 5011b side of the second reflective member 505, and to move between the inside and outside of the cylindrical member 501. Furthermore, the contents here refer to the concept of contents supplied as contents within the cylindrical member 501.

[0292] The second filter 504 is disposed overlappingly on the first end 5011a side of the second reflective member 505. The second filter 504 is disposed on the surface of the first end 5011a side of the second reflective member 505.

[0293] Furthermore, the second reflective member 505 may be positioned in the axial direction relative to the second end 5011b as the first end 5011a side. For example, the second reflective member 505 may be positioned such that the distance from the second end 5011b is 3 cm or less, preferably 1 cm or less, relative to the second end 5011b as the first end 5011a side. That is, the second reflective member 505 may be configured to block the second end 5011b side of the cylindrical member 501. "Configuring the second reflective member 505 to block the second end 5011b side" means configuring the second reflective member 505 to block the second opening 5012b or its vicinity at the second end 5011b. The "second end 5011b side" may, for example, be the second opening 5012b or its vicinity at the second end 5011b.

[0294] Furthermore, the planar shape of the second reflective member 505 only needs to be, for example, the shape and size of the second opening 5012b that can block the second end 5011b.

[0295] Furthermore, the first reflective member 503 and the second reflective member 505 are preferably installed in the cylindrical member 501 in such a way that microwaves introduced into the cylindrical member 501 will not leak to the outside and are enclosed in the cylindrical member 501.

[0296] Furthermore, the arrangement of the first filter 502 to block the first end 5011a side of the cylindrical member 501 can be a configuration where the first filter 502 and the first reflective member 503 are arranged to separate the cylindrical member 501. For example, the first filter 502 can be arranged to separate the interior and exterior of the cylindrical member 501. The configuration to separate the first filter 502 means, for example, that the contents inside the cylindrical member 501 do not pass through the first filter 502 and do not move within the two regions separated by the first filter 502. This is also true for arranging the first reflective member 503 to block the first end 5011a side of the cylindrical member 501, arranging the second filter 504 to block the second end 5011b side of the cylindrical member 501, and arranging the second reflective member 505 to block the second end 5011b side of the cylindrical member 501.

[0297] Furthermore, the tubular column 5 can be a divisible and assembleable component. For example, the cylindrical component 501 can be composed of two or more detachable cylindrical components, with a first filter 502 and a first reflective component 503 installed in the component containing the first end 5011a (not shown), and a second filter 504 and a second reflective component 505 installed in the component containing the second end 5011b (not shown). Furthermore, the construction for detachable cylindrical components is known technology, so detailed descriptions are omitted here. For example, joints can be provided at the connection points of the cylindrical components. Also, at least one of the combination of the first filter 502 and the first reflective component 503 and the combination of the second filter 504 and the second reflective component 505 can be detached from the tubular column 5. Furthermore, the construction of detachable filters, etc., is known technology, so detailed descriptions are omitted here. As described above, the tubular column 5 is a detachable structure, thereby allowing the addition of solid materials such as solid resin that cannot pass through the first filter 502 and the second filter 504 between the first filter 502 and the second filter 504 of the cylindrical member 501.

[0298] Furthermore, a closable cap or plug (not shown) for adding solid resin or the like can be provided on the side of the cylindrical member 501.

[0299] Figure 11 This is a perspective view showing the state in which the tubular column 5 of this embodiment is installed in the main part of the apparatus used for solid-phase synthesis. Figure 11 (a) and its cross-sectional view of the XIb-XIb line ( Figure 11(b)).

[0300] After that, use Figure 11 This describes an example of a method for installing the tubular column 5 in an apparatus used for solid-phase synthesis. A first cover 701a, having an opening 7011, is installed on the bottom surface 7012 to cover the first end 5011a side of the cylindrical member 501. The opening 7011 is provided for supplying and discharging liquids (e.g., solutions used in reactions or cleaning solutions used in cleaning) or gases used in the process. The first cover 701a is installed in a manner that does not create gaps between it and the side of the cylindrical member 501. A connector (not shown) can be pre-installed between the first cover 701a and the cylindrical member 501. Furthermore, a second cover 701b, identical to the first cover 701a, is installed to cover the second end 5011b side of the cylindrical member 501 of the tubular column 5. The opening 7011 of the first cover 701a is provided with a pipe 702a for supplying and discharging liquids or gases used in the process. Furthermore, the opening 7011 of the second cover 701b is equipped with a pipe 702b for supplying and discharging liquids or gases used in the treatment. Then, at least one of the pipes 702a and 702b is connected to a pump (not shown), a valve (not shown), or a container (not shown) into which liquids or gases can be placed.

[0301] For example, liquid or gas is supplied to the first cover 701a via pipe 702a using a pump, thereby supplying liquid or gas into the column 5. Furthermore, the contents of the column 5, i.e., liquid or gas, can be discharged through pipe 702b from the opening 7011 of the second cover 701b. By simultaneously supplying liquid or gas via pipe 702a and discharging the contents of the column 5 via pipe 702b, the contents can flow continuously within the column 5.

[0302] Similarly, for example, liquid or gas can be supplied to the second cover 701b via pipe 702b, thereby supplying liquid or gas into the interior of the column 5. Furthermore, the contents of the column 5, i.e., liquid or gas, can be discharged through pipe 702a from the opening 7011 of the first cover 701a.

[0303] Liquid or gas is supplied through pipe 702b while the contents of the column 5 are discharged through pipe 702a, thereby allowing continuous flow of the contents within the column 5. Furthermore, the term "within the column 5" refers to, for example, the area enclosed by the combination of the first filter 502 and the first reflective member 503 and the combination of the second filter 504 and the second reflective member 505 within the cylindrical member 501. This area can be considered as space.

[0304] Furthermore, the first cover 701a and the second cover 701b can be made of any material. For example, microwave-permeable materials such as synthetic resin can be used, or microwave-reflective materials such as stainless steel can be used.

[0305] Furthermore, instead of installing the first cover 701a connected to the pipe 702a and the second cover 701b connected to the pipe 702b on the cylindrical member 501, the piping (not shown) that can supply and discharge contents can be directly connected to the first end 5011a and the second end 5011b of the cylindrical member 501, respectively.

[0306] A waveguide 801 is connected to the inlet 5013 of the cylindrical member 501, and this waveguide 801 is connected to the microwave oscillator 8. The inlet 5013 and the end of the waveguide 801 can be connected in any way. For example, a connector can be provided on the outside of the cylindrical member 501 and on the outer periphery of the inlet 5013, and the waveguide 801 and the inlet 5013 can be connected through this connector. Alternatively, the flange provided at the end of the waveguide 801 can be fixed with screws to the periphery of the inlet 5013 on the side of the cylindrical member 501. With the above connection, microwaves emitted by the microwave oscillator 8 can be supplied to the column 5 through the waveguide 801 and the inlet 5013. Furthermore, it is preferable to provide a sealing member (not shown) or the like to prevent microwave leakage between the waveguide 801 and the cylindrical member 501. Furthermore, as long as the opening of the waveguide 801 is configured in such a way as to block the inlet 5013, the waveguide 801 does not necessarily need to be installed on the cylindrical member 501. The column 5 and the waveguide 801 can be configured such that the end of the waveguide 801 on the cylindrical member 501 side is connected to the periphery of the inlet 5013 of the cylindrical member 501.

[0307] Furthermore, although the inlet 5013 is connected to the microwave oscillator 8 via the waveguide 801, a coaxial cable and an antenna can be used to connect the inlet 5013 and the microwave oscillator 8 instead of the waveguide 801. Alternatively, the portion of the inlet 5013 of the column 5 can be placed inside the waveguide (not shown) connected to the microwave oscillator 8.

[0308] Furthermore, the microwave oscillator 8 connected to the column 5 can be any microwave oscillator, and the frequency or intensity of the microwaves emitted by the microwave oscillator 8 is not limited. The microwave oscillator 8 is, for example, a microwave oscillator having a magnetron, speed-regulating tube, gyrotron, or semiconductor type oscillator. The frequency of the microwaves emitted by the microwave oscillator 8 can be, for example, 915 MHz, 2.45 GHz, 5.8 GHz, or other frequencies within the range of 300 MHz to 300 GHz. Furthermore, the waveguide 801 can be, for example, a waveguide that corresponds to the frequency of the microwaves emitted by the microwave oscillator 8.

[0309] Furthermore, taking the connection with the aforementioned device as an example, the tubular column 5 can be connected in any manner depending on the device to which it is connected.

[0310] The following is a brief description of the peptide solid-phase synthesis process using column 5 of embodiment 5. Column 5 of embodiment 5 is mounted on... Figure 11 The apparatus shown allows for solid-phase synthesis, which is the same solid-phase synthesis as that performed using the processing apparatus 3 in Embodiment 3 described above. In this case, for example, in a specific example of Embodiment 3, instead of placing the solid resin or the like between the first filter 105 and the second filter 107 of the processing apparatus 3, the solid resin or the like is placed between the first filter 502 and the second filter 504 of the column 5, and instead of supplying liquid or other contents through the second opening 1011b of the processing apparatus 3, the solid resin or the like is supplied through... Figure 11 The tube 702b shown supplies the contents and, instead of discharging the contents through the first opening 1011a of the processing device 4, supplies the contents through the tube 702b. Figure 11 The contents are discharged through pipe 702a, thereby allowing solid-phase synthesis to occur within the cylindrical member 501, which is the same as in the specific example of Embodiment 3 described above. Controlling the supply of contents to pipe 702b or the discharge of contents from pipe 702a can be achieved, for example, by using a pump or nozzle (not shown) installed in pipe 702b or pipe 702a. Furthermore, the contents used for processing, the processing sequence, and the number of processing repetitions are the same as in the specific example of Embodiment 3 described above, so detailed descriptions are omitted here.

[0311] Furthermore, one or more of the processes described above for solid-phase synthesis can be performed while the contents, such as liquid, are flowing within the column, or the processes can be performed without the contents flowing.

[0312] In addition, a nozzle (not shown) for supplying gases such as nitrogen can be provided in the column 5, and one or more of the above-mentioned solid-phase synthesis processes can be performed while the liquid in the column 5 is stirred by bubbles generated by the gas supplied by the nozzle.

[0313] Furthermore, the peptide solid-phase synthesis treatment using column 5 of embodiment 5 described above is one example; peptide solid-phase synthesis other than those described above can also be performed.

[0314] In this embodiment, the first end 5011a and the second end 5011b of the opening of the cylindrical member 501, which is made of microwave reflective material, are blocked. A first reflective member 503 and a second reflective member 505 are provided, and microwaves are introduced into the cylindrical member 501 through the inlet 5013. This prevents microwaves introduced into the cylindrical member 501 from easily penetrating to the outside of the cylindrical member 501, thus efficiently irradiating the microwaves. This, for example, can increase the processing capacity of microwave irradiation.

[0315] Furthermore, the first end 5011a and the second end 5011b are blocked by a first filter 502, a first reflective member 503, a second filter 504, and a second reflective member 505, allowing at least a portion of the contents to pass through. Therefore, microwave irradiation can be performed while the contents flow from the first end 5011a to the second end 5011b within the cylindrical member 501, and vice versa, allowing for flexible handling of various processes. Moreover, regardless of the direction of contents flow, the first end 5011a and the second end 5011b are blocked by the first filter 502 and the second filter 504, respectively, thus preventing the solid resin within the contents of the cylindrical member 501 from flowing out to the outside.

[0316] Furthermore, in the column 5 of this embodiment, the first filter 502 is disposed in an overlapping manner on the first reflective member 503, which is made of a reflective material such as metal. Therefore, the first filter 502 can be reinforced and supported by the first reflective member 503. Thus, for example, the first filter 502, which is not easily disposed separately in a way that separates the cylindrical member 501, or the sheet-like first filter 502, can be disposed in a way that separates the cylindrical member 501. This increases the selectivity of the first filter 502.

[0317] Similarly, the second filter 504 and the second reflective member 505, which is made of a reflective material such as metal, are arranged in an overlapping manner, so the second filter 504 can be reinforced and supported by the second reflective member 505. Therefore, for example, the second filter 504, which is made of a low-hardness material that is not easily arranged separately in a way that separates the cylindrical member 501, or a sheet-like second filter 504, can be arranged separately within the cylindrical member 501. This increases the selectivity of the second filter 504.

[0318] (First variation)

[0319] Figure 12 (a)- Figure 12 (c) is a cross-sectional view illustrating a first modified example of the tubular column of this embodiment, which is equivalent to... Figure 9 (b) is a cross-sectional view.

[0320] The above embodiment describes the case where the first filter 502 is overlapped and disposed on the second end 5011b side of the first reflective member 503. However, as long as the first filter 502 is disposed on the side closer to the second end 5011b of the first reflective member 503, the first filter 502 may not be disposed on the first reflective member 503 in a direct overlapping manner.

[0321] For example, such asFigure 12 As shown in (a), the first filter 502 and the first reflective member 503 can be arranged in a manner that does not directly overlap. Even in this case, the microwaves passing through the first filter 502 are reflected by the first reflective member 503, preventing the microwaves from passing through the first opening 5012a of the first end 5011a to the outside, and the microwaves can still be efficiently utilized for solid-phase synthesis.

[0322] Furthermore, instead of positioning the first filter 502 on the side closer to the second end 5011b than the first reflective member 503, as... Figure 12 As shown in (b), the first filter 502 can be positioned closer to the first end 5011a than the first reflective member 503. Even in this case, the first reflective member 503 reflects microwaves, thereby making it difficult for microwaves to pass through the first opening 5012a of the first end 5011a of the cylindrical member 501, allowing for efficient utilization of microwaves. Furthermore, Figure 12 (b) illustrates an example where the first filter 502 is disposed at the first opening 5012a of the first end 5011a. In this case, the first filter 502 prevents the solid resin inside the cylindrical member 501 from moving to the outside through the first opening 5012a of the first end 5011a of the cylindrical member 501. Therefore, the first reflective member 503 can also be a member that allows the solid resin to pass through. For example, the first reflective member 503 can be a member with multiple holes, which have a shape and size that allow the solid resin to pass through. However, in this case, microwaves are not easily able to irradiate the contents containing the solid resin, which are located between the first reflective member 503 and the first filter 502. Therefore, if the distance between the first reflective member 503 and the first filter 502 is increased, the processing efficiency of solid-phase synthesis may be reduced. Furthermore, in this case, the first filter 502 can also be made of a material other than a microwave-permeable material.

[0323] Furthermore, as an example of the case where the first filter 502 is positioned closer to the first end 5011a than the first reflective member 503, such as... Figure 12 As shown in (c), the first reflective member 503 can be overlapped and disposed on the surface of the second end 5011b side of the first filter 502. Furthermore, Figure 12 (c) illustrates an example where the first filter 502 is disposed at the first opening 5012a of the first end 5011a. In this case, there is no distance between the first reflective member 503 and the first filter 502, which prevents a reduction in the processing efficiency of solid-phase synthesis as described above. Furthermore, the first filter 502 and the first reflective member 503 can be disposed in an overlapping manner, thereby reinforcing and supporting the first filter 502 with the first reflective member 503. As described in the above embodiment or... Figure 12(c) The first filter 502 and the first reflective member 503 are arranged in an overlapping manner, thereby the first reflective member 503 can reinforce the first filter 502 and increase the selectivity of the first filter 502.

[0324] (Second variation)

[0325] Figure 13 (a)- Figure 13 (c) is a cross-sectional view of a second modified example of the tubular column used to illustrate this embodiment, which is equivalent to... Figure 9 (b) is a cross-sectional view.

[0326] For example, in the above embodiment, the second filter 504 is arranged overlapping the second reflective member 505. However, as long as the second filter 504 is arranged on the side closer to the first end 5011a of the second reflective member 505, the second filter 504 may not be directly overlapped with the second reflective member 505.

[0327] For example, such as Figure 13 As shown in (a), the second filter 504 and the second reflective member 505 can be arranged in a manner that does not directly overlap. Even in this case, microwaves passing through the second filter 504 are reflected by the second reflective member 505, preventing microwaves from passing through the second opening 5012b of the second end 5011b to the outside, and microwaves can be efficiently utilized for solid-phase synthesis. However, at this time, microwaves also irradiate the contents that do not contain solid-phase resin, which are located between the second filter 504 and the second reflective member 505. Therefore, compared to the case where the second filter 504 and the second reflective member 505 overlap, microwaves cannot be utilized efficiently. Furthermore, the second filter 504 cannot be reinforced by the second reflective member 505 at this time.

[0328] Furthermore, instead of positioning the second filter 504 on the side closer to the first end 5011a than the second reflective member 505, as... Figure 13 As shown in (b), the second filter 504 can be positioned closer to the second end 5011b than the second reflective member 505. Even in this case, the second reflective member 505 reflects the microwaves, thereby preventing the microwaves from easily passing through the second opening 5012b of the second end 5011b of the cylindrical member 501, allowing for efficient microwave utilization. Furthermore, Figure 13(b) illustrates an example where the second filter 504 is disposed at the second opening 5012b of the second end 5011b. In this case, the second filter 504 prevents the solid resin inside the cylindrical member 501 from moving to the outside through the second opening 5012b on the second end 5011b side of the cylindrical member 501, so the second reflective member 505 can also be a member that allows the solid resin to pass through. For example, the second reflective member 505 can be a member with multiple holes, the multiple holes having a shape and size that allow the solid resin to pass through. However, in this case, microwaves are unlikely to irradiate the contents containing the solid resin, which are located between the second reflective member 505 and the second filter 504. Furthermore, in this case, the second filter 504 can be made of a material other than a microwave-permeable material.

[0329] Furthermore, as an example of the second filter 504 being positioned closer to the second end 5011b than the second reflective member 505, such as... Figure 13 As shown in (c), the second reflective member 505 can be overlapped on the surface of the second filter 504 on the side of the first end 5011a. Furthermore, Figure 13 (c) shows an example where the second filter 504 is disposed at the second opening 5012b of the second end 5011b. In this case, there is no distance between the second reflective member 505 and the second filter 504, which prevents the reduction in processing efficiency of solid-phase synthesis as described above. Furthermore, by arranging the second filter 504 and the second reflective member 505 in an overlapping manner, the second reflective member 505 can reinforce and support the second filter 504. As described in the above embodiment or... Figure 13 (c) The second filter 504 and the second reflective member 505 are arranged in an overlapping manner, thereby the second reflective member 505 can reinforce the second filter 504 and increase the selectivity of the second filter 504.

[0330] Furthermore, the second filter 504 and the second reflective member 505 of the column 5 in the above-described embodiment 5 and its first modification can also be the same as those in the second modification described above. Figure 13 (a)- Figure 13 (c) Any combination of the second filter 504 and the second reflective member 505 described separately. Furthermore, the first filter 502 and the first reflective member 503, as described in Embodiment 5 and its second variation, may also use the components described in the first variation. Figure 12 (a)- Figure 12 (c) Any combination of the first filter 502 and the first reflective member 503 described respectively. For example, the column 5 may have any combination of the first filter 502 and the first reflective member 503 described in the above embodiment 5 and its first variation, and any combination of the second filter 504 and the second reflective member 505 described in the above embodiment 5 and its second variation.

[0331] (Implementation Form 6)

[0332] The column of this embodiment is as follows: the first filter 502 and the first reflective member 503, as well as the second filter 504 and the second reflective member 505 in the column 5 described in the above embodiment 5 are replaced by microwave reflective filters.

[0333] Figure 14 This is a perspective view showing an example of a tubular column in this embodiment. Figure 14 (a) and its cross-sectional view of line XIVb-XIVb. Figure 14 (b)). But Figure 14 (b) Omits the cross-section of the valve, etc.

[0334] The column 6 in this embodiment includes a cylindrical member 501, a first reflective filter 603, and a second reflective filter 605. The cylindrical member 501 has a first end 5011a, a second end 5011b, an inlet 5013, and a microwave-permeable member 5014. Furthermore, the configuration other than the first reflective filter 603 and the second reflective filter 605 is the same as that in Embodiment 5 described above, so detailed descriptions are omitted here.

[0335] The first reflective filter 603 is a filter formed by integrating the first filter 502 and the first reflective member 503. It is a filter that separates solid resin from the contents of the cylindrical member 501 and reflects microwaves introduced through the inlet 5013. The first reflective filter 603 can be, for example, a metal mesh made of stainless steel or a metal filter in which multiple metal meshes are overlapped and sintered together. The first reflective filter 603 can utilize the same components as the first reflective filter 205; detailed descriptions are omitted here.

[0336] The first reflective filter 603 is disposed at the first end 5011a of the cylindrical member 501 in a manner that blocks the first end 5011a. Furthermore, since the first reflective filter 603 is an integrated component of the first filter 502 and the first reflective member 503, it only needs to be disposed in the same manner as the first filter 502 and the first reflective member 503, blocking the first end 5011a. For example, the first reflective filter 603 only needs to be disposed at a position closer to the second end 5011b than the first end 5011a. For example, the distance between the first reflective filter 603 and the first end 5011a can be less than 3 cm, preferably less than 1 cm, at a position closer to the second end 5011b than the first end 5011a.

[0337] The second reflective filter 605 is a filter formed by integrating the second filter 504 and the second reflective member 505. It is a filter that separates solid resin from the contents of the cylindrical member 501 and reflects microwaves introduced into the cylindrical member 501 through the inlet 5013. The second reflective filter 605 can use the same components as the first reflective filter 603. The first reflective filter 603 and the second reflective filter 605 can use the same components or different components.

[0338] The second reflective filter 605 is disposed at the second end 5011b of the cylindrical member 501 in a manner that blocks the second end 5011b. Furthermore, since the second reflective filter 605 is an integral component of the second filter 504 and the second reflective member 505, it only needs to be disposed in the same manner as the second filter 504 and the second reflective member 505, blocking the second end 5011b. For example, the second reflective filter 605 only needs to be disposed at a position relative to the second end 5011b that is closer to the first end 5011a. For example, the distance between the second reflective filter 605 and the second end 5011b can be less than 3 cm, preferably less than 1 cm, and disposed at a position closer to the first end 5011a than the second end 5011b.

[0339] Furthermore, when it is not convenient to place the first reflective filter 603 separately within the cylindrical member 501, a reinforcing frame or the like can be installed within the first reflective filter 603. The same applies to the second reflective filter 605.

[0340] Even in this embodiment, similar to the embodiments described above, the microwaves introduced into the cylindrical member 501 do not easily penetrate to the outside of the cylindrical member 501, thus efficiently irradiating the microwaves. Furthermore, by providing a first reflective filter 603 and a second reflective filter 605, the solid resin can be separated from the contents.

[0341] Furthermore, the first end 5011a and the second end 5011b are blocked by a first reflective filter 603 and a second reflective filter 605, respectively, allowing at least a portion of the contents to pass through. Therefore, microwave irradiation can be performed while the contents flow from the first end 5011a to the second end 5011b within the cylindrical member 501, or while the contents flow from the second end 5011b to the first end 5011a within the cylindrical member 501, allowing for flexible adaptation to various processing methods. Moreover, regardless of the direction of contents flow, the first end 5011a and the second end 5011b are blocked by the first reflective filter 603 and the second reflective filter 605, respectively, thus preventing the solid resin within the contents of the cylindrical member 501, which is the object of separation, from flowing out of the cylindrical member 501 to the outside.

[0342] Alternatively, the first reflection filter 603 of embodiment 6 described above can be used instead. Figure 9 and Figure 13 (a)- Figure 13 (c) The first filter 502 and the first reflective member 503 of the column 5 described in Embodiment 5 and its second variation respectively can be replaced by the second reflective filter 605 of Embodiment 6 described above. Figure 9 and Figure 12 (a)- Figure 12 (c) The second filter 504 and the second reflective member 505 described in Embodiment 5 and its first variation are shown respectively. For example, the column only needs to have the combination of the first filter 502 and the first reflective member 503 and any one of the first reflective filter 603 described in Embodiment 5 and its first variation, as well as the combination of the second filter 504 and the second reflective member 505 and any one of the second reflective filter 605 described in Embodiment 5 and its second variation.

[0343] Furthermore, in all the above embodiments, the inlet 5013 can be located between the first reflective member 503 and the second reflective member 505 on the side of the cylindrical member 501, and its position is not limited. The positions of the inlet 5013, the first reflective member 503, and the second reflective member 505 mentioned here refer, for example, to their axial positions on the cylindrical member 501. Furthermore, when a first reflective filter 603 is used instead of the first filter 502 and the first reflective member 503, the first reflective member 503 can simply be interpreted as the first reflective filter 603. Similarly, when a second reflective filter 605 is used instead of the second filter 504 and the second reflective member 505, the second reflective member 505 can simply be interpreted as the second reflective filter 605.

[0344] For example, such as Figure 12 As shown in (a), when the first reflective member 503 is disposed on the first end 5011a side relative to the first filter 502, and the first reflective member 503 and the first filter 502 are separately disposed in a non-overlapping manner, the inlet 5013 can be disposed between the first filter 502 and the first reflective member 503.

[0345] Also, such as Figure 13 As shown in (a), when the second reflective member 505 is disposed on the second end 5011b side relative to the second filter 504, and the second reflective member 505 and the second filter 504 are separately disposed in a non-overlapping manner, the inlet 5013 can be disposed between the second filter 504 and the second reflective member 505.

[0346] Furthermore, embodiments 5 and 6 describe an example where a cylindrical member 501, serving as a container, has an inlet 5013 through which microwaves are introduced (in other words, irradiated) into the cylindrical member 501. However, the cylindrical member 501 only needs to have an emitting portion (not shown) for irradiating microwaves into it, and it is sufficient that microwaves can be irradiated into the cylindrical member 501 through this emitting portion. The emitting portion is the part that emits the microwaves irradiated into the cylindrical member 501. The emitting portion can have any structure or shape, as long as it can, for example, emit (in other words, introduce) microwaves irradiated by an irradiation device into the cylindrical member 501. The emitting portion can be, for example, the inlet 5013 of embodiments 5 and 6. Furthermore, the position of the emitting portion can be the position where the microwaves are emitted. Furthermore, the description of the positional relationship of the first filter 502 or the first reflective member 503, the second filter 504, the second reflective member 505, etc., relative to the inlet 5013 can be interpreted as a description of the positional relationship relative to the ejection position.

[0347] Furthermore, in the aforementioned tubular column 5, the following scenario will be described: the inlet and other exit portions are provided on the side of the cylindrical member 501; the first filter 502 is configured to block the first end 5011a side of the cylindrical member 501; the first reflector 503 is configured to block the first end 5011a side of the cylindrical member 501; the second filter 504 is configured to block the second end 5011b side of the cylindrical member 501; and the second reflector 505 is configured to block the second end 5011b side of the cylindrical member 501. However, in the tubular column 5, for example, the first filter 502 is configured to block the second end 5011b side of the cylindrical member 501. The system can be configured such that a cylindrical member 501, which serves as a container, is separated from the first end 5011a by the cylindrical member 501; the first reflective member 503 is positioned between the first end 5011a and the second end 5011b by separating the cylindrical member 501; the second filter 504 is positioned between the first filter 502 and the first reflective member 503 by separating the cylindrical member 501 by separating the second end 5011b by separating the first filter 502 and the first reflective member 503; and the second reflective member 505 is positioned between the first filter 502 and the second end 5011b by separating the cylindrical member 501. The ejection position can be, for example, the axial position of an exit portion such as an inlet 5013. In this case, the first filter 502 or the first reflective member 503 can be installed in the first opening 5011a, and the second filter 504 or the second reflective member 505 can be installed in the second opening 5011b. Even in this situation, microwaves irradiated into the cylindrical member 501 are not easily emitted to the outer side of the cylindrical member 501, the first reflector 503, and the second reflector 505, but microwave irradiation can still be efficient. In addition, solid materials such as solid resin that are the objects of separation can simply be supplied between the first filter 502 and the second filter 504.

[0348] For example, in the above-mentioned column 6, as long as the first reflective filter 603 is arranged in a manner that separates the cylindrical member 501 on the side closer to the first end 5011a than the ejection position, and the ejection position is arranged between the first end 5011a and the second end 5011b, the second reflective filter 605 is arranged in a manner that separates the cylindrical member 501 on the side closer to the second end 5011b than the ejection position.

[0349] Furthermore, the tubing columns 5 and 6 described in embodiments 5 and 6 above can be installed in and utilized in a processing apparatus, which is as follows: Figure 11 The processing apparatus shown for solid-state synthesis or other treatments can also be part of such a processing apparatus. For example, the processing apparatus can be a processing apparatus that irradiates columns 5 and 6, the irradiation apparatus having a microwave oscillator 8 and a waveguide 801, etc. Furthermore, columns 5 and 6, etc., can be processing apparatuses for solid-state synthesis or other treatments.

[0350] Furthermore, when synthesizing a peptide using the processing apparatus or column described in the above embodiments in a solid-phase synthesis, the number of amino acids in the synthesized peptide is only two or more, and the number is not limited. For example, the number of amino acids in the synthesized peptide can be two or more and less than 20, or more than 20 and less than 50, or more than 50. For example, the peptide synthesized using the processing apparatus or column described in the above embodiments in a solid-phase synthesis can be an oligopeptide with about two to about 20 amino acids, or a polypeptide with about 20 or more amino acids. Also, the synthesized peptide can be a polypeptide with about 50 or more amino acids bonded together, i.e., a protein. Furthermore, the type of amino acids used to synthesize the peptide or the sequence order of the amino acids used to synthesize the peptide is not limited.

[0351] Furthermore, the processing apparatus or column described in the above embodiments can be used for the solid-phase synthesis of one or more peptides used in the preparation of any substance. For example, the processing apparatus of embodiments 1 and 2 can be used for the solid-phase synthesis of one or more peptides (e.g., oligopeptides or polypeptides) used in the preparation of antibodies. For example, the processing apparatus or column of the above embodiments can be used for the solid-phase synthesis of one or more peptides used as antibody raw materials, or for the solid-phase synthesis of one or more peptides or a portion thereof constituting an antibody (e.g., one or more polypeptides or a portion thereof constituting an antibody).

[0352] Furthermore, the processing apparatus or column described in the above embodiments can be used for processes including solid-phase synthesis of peptides. For example, after a specific peptide is synthesized in the solid phase using the processing apparatus or column described in the above embodiments, a specific substance (e.g., sugar) can be further bonded to the specific peptide synthesized in the solid phase using the processing apparatus or column. For example, the processing apparatus or column described in the above embodiments can be used for the solid-phase synthesis of glycopeptides.

[0353] Furthermore, while the above embodiments describe the use of a processing apparatus or column for solid-phase synthesis of peptides already bonded to a solid-phase resin, the processing performed using the apparatus or column is not limited to peptide solid-phase synthesis; it can also be used for processes other than peptide solid-phase synthesis. For example, it can be used for solid-phase synthesis other than peptide solid-phase synthesis, or for processes other than solid-phase synthesis.

[0354] For example, the processing apparatus or column described in the above embodiments can be used in processes including the step of separating solids from the contents of container 101, container 301, or cylindrical member 501, such as in solid-phase synthesis. The solids separated from the contents are, for example, solids present between the first filter 105 and the second filter 107, solids present between the first reflective filter 205 and the second reflective filter 206, solids present between the first filter 502 and the second filter 504, or solids present between the first reflective filter 603 and the second reflective filter 605. For example, when processing apparatus 1 to 4, column 5, and column 6 are used for processes other than peptide solid-phase synthesis that include such a solid separation step, the description of the solid resin in processing apparatus 1 to 4, column 5, and column 6 in the above embodiments can be interpreted as a description of the object to be separated, i.e., the solids. For example, the configuration determined by the size of the solid resin in each of the above embodiments can be changed to the configuration determined by the size of the object to be separated, i.e., the solid object.

[0355] For example, the first filter 105, the second filter 107, the first reflective filter 205, and the second reflective filter 206 of the processing apparatus described in the above embodiments, or the first filter 502, the second filter 504, the first reflective filter 603, and the second reflective filter 605 of the tubular columns 5 and 6 described in the above embodiments, can be used as filters with multiple holes for separating solids that are to be separated. In this way, the processing apparatus or tubular column can be used for a process that includes the step of separating solids that are to be separated from the contents of the container 101, the container 301, or the cylindrical member 501.

[0356] The contents of the container 101 and the cylindrical member 501 are, for example, liquid contents. The liquid in the contents can be, for example, a raw material or a solvent, and its composition is not limited. The liquid can be, for example, a solution in which a solvent dissolves a substance. The contents of the container 101 may contain, for example, a liquid and solids that are to be separated. The contents may be, for example, a suspension of solids that are to be separated. The solids referred to here are, for example, in a solid state. Furthermore, the solids may be non-flowing and can be separated by a filter or the like. The solids may be, for example, gel-like. The solids that are to be separated preferably have a shape or size that, for example, is flowable in the liquid contents. The solids that are to be separated are, for example, granular, fragmented, or powdered solids.

[0357] Furthermore, the processing apparatus and tubing described in the above embodiments are devices that can be used for processing, such as the step of irradiating a container or cylindrical member with microwaves and the step of separating the solid material to be separated from the liquid contents within the container or cylindrical member. Peptide solid-phase synthesis is also an example of this type of processing.

[0358] For example, even if microwaves are irradiated and processed in the region of the container 101 of the processing device 1 that is closer to the first end 1015a of the first filter 105, the solids present in this region are separated from the contents by the first filter 105 after processing and remain in the container 101 as a product or intermediate product of the processing. Therefore, irradiating the region with microwaves and processing the solids is sometimes futile. However, by configuring the first reflector 106, microwaves can be made less likely to irradiate the region of the first filter 105 that is closer to the first end 1015a than the first end 1015a of the first filter 105 used to separate the solids, and microwaves can be made less likely to irradiate regions where solids cannot be separated or where there are no solids, thus allowing for efficient use of microwaves. This is also true when processing devices 2 to 4, or the column 5 or column 6 are used for processing that includes the step of separating solids.

[0359] In the processing apparatus of embodiments 1-4 described above, the solid matter to be separated using the first filter 105, the second filter 107, the first reflective filter 205, and the second reflective filter 206 can be, for example, solid matter contained in the contents before microwave irradiation treatment, or solid matter generated during microwave irradiation treatment within container 101 or container 301. The solid matter to be separated using the first filter 502, the second filter 504, the first reflective filter 603, and the second reflective filter 605 of the tubular column in embodiments 5 and 6 described above is the same.

[0360] The solid material that is contained within the contents before microwave irradiation and is the object of separation includes, for example, solid catalysts used in fluidized bed processes, substrates with dimensions that allow for adjustment of microwave absorption flowability, adsorbent materials for adsorbing other substances, or solid monomers. Examples of fluidized bed solid catalysts include, for instance, palladium-on-carbon (Pd / C) catalysts, platinum-on-carbon (Pt / C) catalysts, solid acid catalysts, and zeolite-based catalysts. Furthermore, the solid material that is contained within the contents before microwave irradiation and is the object of separation can also be solid impurities.

[0361] The solid that is separated is generated by microwave irradiation or other processes. For example, it can be a solid generated from the liquid in the contents of a microwave-irradiated container 101, container 301 or cylindrical member 501 through a chemical reaction or other process. It can also be a solid generated from the solidification or crystal growth of the liquid in the contents of a microwave-irradiated container 101, container 301 or cylindrical member 501.

[0362] Furthermore, the so-called solid material generated by microwave irradiation or similar treatments can be, for example, a solid material generated by chemical reaction between solid material contained in the contents before microwave irradiation and other substances within a microwave-irradiated container 101, container 301, or cylindrical member 501; it can also be a solid material that decomposes solid material contained in the contents before microwave irradiation and similar treatments within a microwave-irradiated container 101, container 301, or cylindrical member 501 through chemical reaction; or it can be a solid material generated by irradiating the surface of a solid material contained in the contents before microwave irradiation and similar treatments with microwaves, causing the same or different substances to adsorb, adhere, and grow. In addition, the other or the same substances mentioned herein can be solids, liquids, or gases. Furthermore, the solid material contained in the contents before being subjected to microwave irradiation or other treatments is preferably placed between the first filter 105 and the second end 1015b or between the first reflective filter 205 and the second end 1015b in the container 101, between the first filter 105 and the second filter 107 or between the first reflective filter 205 and the second reflective filter 206 in the container 301, or between the first filter 502 and the second filter 504 or between the first reflective filter 603 and the second reflective filter 605 in the cylindrical member 501.

[0363] The solids to be separated can be solids that are the object of processing using the processing apparatus or column described in the above embodiments, or solids generated by the processing, etc. They can also be solids used in processing such as catalysts or substrates, or unwanted substances, or a mixture of two or more of the above. The size of the solids to be separated is not limited. For example, the solids to be separated can be precipitates. The solids to be separated can be suspended matter or sediment in the contents. When the processing performed in the processing apparatus or column is peptide solid-phase synthesis, the solids to be separated are, for example, solid-phase resins.

[0364] The process of separating solids from contents using the processing apparatus 1 to processing apparatus 4 as described above can be, for example, a process that includes one or more steps of separating solids that are the objects of separation from the contents. For example, the process performed using such processing apparatus 1 to processing apparatus 4 can be a process that performs at least one of the following two groups of processes. The first group is: a step of irradiating contents containing liquid with microwaves; a step of adding liquid or the like as contents into container 101 after using any one of the first filter 105, second filter 107, first reflective filter 205 and second reflective filter 206 to separate solids that are the objects of separation from the contents; a step of irradiating the container 101 with microwaves; and a step of using any one of the first filter 105, second filter 107, first reflective filter 205 and second reflective filter 206 to separate solids that are the objects of separation from the contents. The second group consists of: the step of adding liquid or the like as contents into container 101; and the step of using any one of the first filter 105, the second filter 107, the first reflective filter 205, and the second reflective filter 206 to separate the solids that are to be separated from the contents. This process is also the same when using the aforementioned tubing 5 or tubing 6.

[0365] The following describes specific examples of using the processing apparatus 1 to processing apparatus 4 described in the above embodiments for processing other than peptide solid-phase synthesis.

[0366] (A) Examples of processes that use solids and liquids to generate solids.

[0367] The processing apparatus 1 to 4 described in the above embodiments can be used for, for example, the synthesis of zeolites via hydrothermal synthesis. For example, the Al (aluminum) source can be metallic aluminum, sodium aluminate, aluminum hydroxide, etc., and the Si (silicon) source can be silica powder or silica gel, etc. The reaction is carried out in the presence of an alkali (sodium hydroxide or potassium hydroxide) and water, under high temperature and high pressure hot water conditions caused by microwave irradiation, thereby generating a crystalline aluminosilicate zeolite polycrystalline film. After processing, the solid material, i.e., the zeolite polycrystalline film, is separated by a first filter 105 or a first reflective filter 205, thereby separating the generated substance from the contents. At this time, the first filter 105, the second filter 107, the first reflective filter 205, and the second reflective filter 206 are filters with multiple pores for separating the solid material to be separated, i.e., the zeolite polycrystalline film.

[0368] (B) Examples of treatments using catalysts that utilize solids

[0369] (B-1)

[0370] The processing apparatus 1 to 4 described in the above embodiments can be used, for example, for the production of methyl esters that can be used as biodiesel fuel. For example, a substance containing a large amount of waste cooking oil or other oils (triglycerides) and a fluidized bed of solid catalyst is placed in container 101. Microwaves are irradiated into container 101, thereby simultaneously carrying out transesterification and esterification reactions of the aforementioned substances within one container, and generating methyl esters. The solid catalyst used here is, for example, a mixed solid catalyst composed of a substance having a high dielectric loss coefficient and magnetic loss coefficient suitable for the irradiated microwaves and a substance having reactive sites. In this process, the substance to be processed is placed in container 101, and the solid catalyst is placed above the first filter 105 or the first reflective filter 205, irradiated with microwaves, and after the generation of the product is completed, the solid matter, i.e., the solid catalyst, is separated by the first filter 105 or the first reflective filter 205, thereby removing the solid catalyst from the contents containing the generated substance.

[0371] Furthermore, the first filter 105, the second filter 107, the first reflective filter 205, and the second reflective filter 206 are filters having multiple pores, which are used to separate the solids that are the objects to be separated. This is also the case in the process using other fluidized beds of solid catalysts described below.

[0372] (B-2)

[0373] The processing apparatus 1 to 4 described in the above embodiments can be used for, for example, Pd (palladium) solid catalyst reactions represented by Suzuki-Miyaura coupling. This process involves, for example, using halogen compounds and organoboronoxy compounds as raw materials, and employing a Pd / C (palladium / carbon) solid catalyst in a fluidized bed of activated carbon or similar material with high microwave absorption energy. Microwaves are irradiated in a solvent with low microwave absorption energy, such as toluene, and cross-coupling occurs, thereby generating substances with aromatic ring-aromatic ring bonds, such as biaromatic compounds. In this process, the substance to be processed is placed in container 101, and the solid catalyst is placed in the same location as the solid resin described above. Microwaves are irradiated, and after the generation of the product is completed, the solid material, i.e., the solid catalyst, is separated by a first filter 105 or a first reflective filter 205, thereby removing the solid catalyst from the contents containing the generated substance.

[0374] (B-3)

[0375] The processing apparatus 1 to processing apparatus 4 described in the above embodiments can be used for, for example, microwave irradiation of a solvent-free reaction on a solid-phase support such as alumina or silica gel. For example, a fluidized bed solid catalyst is mixed with a porous solid-phase support such as alumina or silica gel, a catalyst, and an alkali. Using a mixture of raw materials, the fluidized bed solid catalyst is irradiated with microwaves in a solvent-free environment to perform various organic syntheses, thereby obtaining various synthetic products. In this process, the solid catalyst is placed in the same location as the aforementioned solid resin, irradiated with microwaves, and after the generation of the product is completed, the solid material, i.e., the solid catalyst, is separated by a first filter 105 or a first reflective filter 205, thereby removing the solid catalyst from the contents containing the generated substance.

[0376] (C) Examples of processing using microwave bases

[0377] The processing apparatus 1 to processing apparatus 4 described in the above embodiments can be used, for example, in a synthesis reaction that utilizes a microwave substrate (solid) such as SiC (silicon carbide), carbon, or ferrite, which readily absorbs microwaves. For example, by irradiating and heating with microwaves in a solvent such as toluene, where the raw material and a small piece of microwave substrate coexist, various synthetic products can be obtained. In this process, the raw material is placed into container 101, and the microwave substrate is placed in the same location as the solid resin described above. Microwaves are then irradiated, and after the process is completed, the solid, i.e., the microwave substrate, is separated by a first filter 105 or a first reflective filter 205, thereby removing the microwave substrate from the contents containing the generated substance. The first filter 105, the first reflective filter 205, the second filter 107, and the second reflective filter 206 used here are filters with multiple pores for separating the microwave substrate, which is the solid to be separated.

[0378] (D) Examples of solid-phase synthesis other than peptide solid-phase synthesis

[0379] For example, the processing apparatus 1 to processing apparatus 4 described in the above embodiments can be used for solid-phase synthesis using solid-phase resins other than peptide solid-phase synthesis, such as solid-phase synthesis of nucleotide chains like DNA. Solid-phase synthesis other than peptide solid-phase synthesis is a known technique, so detailed descriptions are omitted here. For example, solid-phase synthesis of nucleotide chains like DNA is a known technique, so detailed descriptions are omitted here.

[0380] Furthermore, when the processing apparatus 1 to processing apparatus 4 described in the above embodiments are used for solid-phase synthesis other than peptide solid-phase synthesis, the description of the solid-phase resin for peptides in the above embodiments can be interpreted as, for example, the description of the solid-phase resin used in solid-phase synthesis other than peptides. For example, when used for solid-phase synthesis other than peptide solid-phase synthesis, the first filter 105, the second filter 107, the first reflective filter 205, and the second reflective filter 206 that separate the solid-phase resin used in peptide solid-phase synthesis from the contents can be either the first filter 105 or the first reflective filter 205 that can separate the solid-phase resin used in solid-phase synthesis other than peptides from the contents. This is also the same as the second filter 107 and the second reflective filter 206 when processing apparatus 3 and processing apparatus 4 of embodiments 3 and 4 are used for solid-phase synthesis other than peptides. For example, the plurality of pores of the first filter 105, the second filter 107, the first reflective filter 205, and the second reflective filter 206 can be any size that cannot allow the solid-phase resin used in solid-phase synthesis other than peptides contained in the contents to pass through, and is any size that allows the contents other than the solid resin to pass through. Also, will Figure 3 (b) or Figure 3 (c) When the processing apparatus 1 of the modified embodiment 1 shown is used for solid-phase synthesis other than peptide solid-phase synthesis, the first reflective member 106 that allows the solid-phase resin used in peptide solid-phase synthesis to pass through can be replaced, for example, with a first reflective member 106 that is interpreted as allowing the solid-phase resin used in solid-phase synthesis other than peptide to pass through. For example, the first reflective member 106 can simply use an aperture with a size that allows the solid-phase resin used in solid-phase synthesis other than peptide to pass through. Furthermore, Figure 4 When the processing apparatus 2 of Embodiment 2 is used for solid-phase synthesis other than peptide solid-phase synthesis, it has a first reflective filter 205 with multiple pores for separating the solid-phase resin used in peptide solid-phase synthesis from the contents. Any first reflective filter 205 with multiple pores for separating the solid-phase resin used in solid-phase synthesis other than peptide solid-phase synthesis from the contents is acceptable. Furthermore, the materials of the first filter 105, second filter 107, first reflective member 106, second reflective member 108, first reflective filter 205, and second reflective filter 206, etc., can be the same as those of the processing apparatus 1 to processing apparatus 4 of the aforementioned embodiments.

[0381] Furthermore, when the processing apparatus 1 to processing apparatus 4 described in each embodiment are used for processing other than solid-phase synthesis, the description of the solid-phase resin in each embodiment can be interpreted as a description of the solids contained in the contents of container 101 that are the objects to be separated, just as it is when used for solid-phase synthesis other than the peptides described above.

[0382] Furthermore, the tubular column described in Embodiment 5 or Embodiment 6 can be used for processes other than peptide solid-phase synthesis performed using the processing apparatus 1 to processing apparatus 4 described above, and the same effect as described above can be achieved even in this case. In addition, in this case, the container 101, first filter 105, second filter 107, first reflective member 106, second reflective member 108, first reflective filter 205, and second reflective filter 206 described above can be replaced by reinterpreting them as cylindrical member 501, first filter 502, second filter 504, first reflective member 503, second reflective member 505, first reflective filter 603, and second reflective filter 605, respectively.

[0383] Furthermore, when the column 5 or 6 described in Embodiments 5 or 6 is used for solid-phase synthesis other than peptide solid-phase synthesis, the descriptions of the solid-phase resin for peptides in the above embodiments can be interpreted as, for example, descriptions of the solid-phase resin used in solid-phase synthesis of other peptides. For example, when the column 5 or 6 is used for solid-phase synthesis of nucleotide chains, the descriptions of the solid-phase resin for peptides in the above embodiments can be interpreted as, for example, descriptions of the solid-phase resin used in solid-phase synthesis of nucleotide chains. Furthermore, when the column 5 or 6 is used for processing other than solid-phase synthesis, similarly to when it is used for solid-phase synthesis of other peptides, the descriptions of the solid-phase resin in the above embodiments can be interpreted as descriptions of the solids contained in the contents of the cylindrical member 501 that are the objects of separation.

[0384] (Implementation Form 7)

[0385] The following description will refer to the implementation form 1 described above. Figure 1 The processing apparatus 1 shown is used for solid-phase synthesis processes other than peptide solid-phase synthesis, namely, solid-phase synthesis of nucleotide chains by microwave irradiation. Solid-phase synthesis of nucleotide chains by microwave irradiation includes, for example, solid-phase synthesis of nucleotide chains including one or more steps of microwave irradiation.

[0386] Furthermore, the description of the solid-phase resin in Embodiment 1 above can be interpreted, for example, as a description of the solid-phase resin used in the solid-phase synthesis of nucleotide chains. For example, Figure 1When the processing apparatus 1 of Embodiment 1 is used for solid-phase synthesis of nucleotide chains, the first filter 105 that separates the solid-phase resin used for peptide solid-phase synthesis from the contents need only be a first filter 105 that separates the solid-phase resin used for nucleotide chain solid-phase synthesis from the contents. For example, the plurality of pores of the first filter 105 need only be of a size that prevents the solid-phase resin used for solid-phase synthesis of nucleotide chains contained in the contents from passing through, and that allows other solid-phase resins used for solid-phase synthesis of nucleotide chains in the contents to pass through. Furthermore, the materials of the first filter 105, the first reflective member 106, etc., need only be the same as those of the processing apparatus 1 of the above embodiment.

[0387] A nucleotide chain refers to a combination of two or more nucleotides bonded together. For example, a nucleotide chain can be composed of two or more nucleotides bonded together by a phosphodiester bond. The number of nucleotides constituting a nucleotide chain is not limited if there are two or more nucleotides. A nucleotide chain can be, for example, an oligonucleotide with approximately 20 or fewer nucleotides bonded together, or a polynucleotide with more than 20 nucleotides bonded together. The types of bases or the base sequence of the nucleotides constituting a nucleotide chain are not limited. The sugars contained in the nucleotides constituting a nucleotide chain can be, for example, deoxy-D-ribose, D-ribose, or other sugars. A nucleotide chain can be, for example, a combination of multiple ribonucleotides or deoxyribonucleotides bonded together. A nucleotide chain can be, for example, nucleic acids such as DNA (deoxyribonucleic acid) or RNA (ribonucleic acid). Furthermore, in the nucleotide chain synthesized in this embodiment, the nucleotide ends bonded to the side opposite to the side bonded to the solid resin phase are either bonded with protecting groups (not shown in the figure) or are in an unaddition state such as phosphate groups, thereby having a structure different from the ends of ordinary nucleotides.

[0388] Solid-phase synthesis methods that bond nucleotides to solid-phase resins and elongate nucleotide chains include the phosphotriester method, the H-phosphonate method, and the phosphoramidite method. Currently, the phosphoramidite method is the most commonly used solid-phase synthesis method. The phosphoramidite method consists of four steps as shown in (I)-(IV) below, and these steps are repeated to synthesize the target nucleotide chain.

[0389] The solid-phase resin used for solid-phase synthesis of nucleotide chains is the same material and size as the solid-phase resin used for peptide solid-phase synthesis as described in the above embodiments. However, any solid-phase resin can be used as long as it is applicable to solid-phase synthesis of nucleotide chains.

[0390] The following is a brief explanation of the phosphite monoamide method. Furthermore, the solid-phase resin used in the solid-phase synthesis can be the same as the solid-phase resin used in the embodiments described above. However, the size or material of the solid-phase resin can be the same as or different from the solid-phase resin used in the peptide solid-phase synthesis.

[0391] (I) The protecting group of the 5'-OH group of the nucleoside that is bonded to the solid resin, namely the DMTr (dimethoxytriphenylmethyl) group, is removed by deprotecting agents such as trichloroacetic acid solution.

[0392] (II) Condense the nucleoside phosphite monoamide compound activated by an activator such as tetrazolium with the nucleoside bonded to the above-mentioned deprotected solid resin.

[0393] (III) Acetylate and protect the 5'-OH group of the unbonded nucleoside phosphite monoamide compound that has been bonded to the solid resin with acetic anhydride or the like.

[0394] (IV) Oxidize triphosphite to triphosphate by oxidizing agents such as iodine.

[0395] Furthermore, steps (I) to (IV) above are, for example, processes involving microwave irradiation. Steps (I) to (IV) above are repeated once or more to synthesize a nucleotide chain having the target sequence. Then, concentrated ammonia or the like is added to the solid-phase resin to which the nucleotide chains are bonded, causing the solid-phase resin to cleave the nucleotides and deprotect them.

[0396] The following description will refer to the implementation form 1 described above. Figure 1 The processing apparatus 1 shown is an example of a process used in the solid-phase synthesis of one type of nucleotide chain, namely DNA. Here, solid-phase synthesis using the phosphite monoamide method is given as an example and explained. However, the solid-phase DNA synthesis described here is only one example, and the processing details can be appropriately modified.

[0397] With the first valve 103a closed, and without discharging the contents through the first opening 1011, a solid resin with 5'-terminal hydroxyl groups protected by DMTr groups and a trichloroacetic acid solution using dichloromethane or the like are supplied into the container 101 through the second opening 1012. While nitrogen gas is supplied to the contents of the container 101 and the mixture is stirred with bubbles, the contents are irradiated with 915 MHz microwaves by the irradiation device 102 to perform deprotection. This step is equivalent to step (I) described above. After deprotection is complete, if the first valve 103a is opened, the deprotection solution in the contents of the container 101 is discharged to the outside. The deprotected solid resin in the contents of the container 101 is separated from the deprotection solution and remains on the upper surface 1051 of the first filter 105.

[0398] After acetonitrile is supplied into container 101 through the second opening 1012 and held therein, acetonitrile is discharged through the first opening 1011 to clean the deprotected solid resin remaining on the upper surface 1051 of the first filter 105. This cleaning is performed multiple times.

[0399] Subsequently, in order to condense the 5'-terminal deprotected nucleoside on the solid resin with the nucleoside phosphite monoamide, 1H-tetrazole as an activator, the 5'-terminal protected nucleoside phosphite monoamide compound used for coupling, and acetonitrile as a solvent are supplied to container 101 through the second opening 1012. The 1H-tetrazole and the nucleoside phosphite monoamide compound are dissolved in the acetonitrile, and microwaves are applied by irradiation device 102 to bond the nucleoside phosphite monoamide to the 5'-terminal deprotected nucleoside already bonded to the solid resin, thereby obtaining a phosphite triester. This step is equivalent to step (II) described above. After the connection of the nucleoside phosphite monoamide is completed, if the first valve 103a is opened, the bonding solution is discharged from container 101. The solid resin with the 5'-terminal protected phosphite triester bonded is not discharged but remains on the upper surface 1051 of the first filter 105. The cleaning process using acetonitrile is repeated multiple times in the same manner as described above.

[0400] Subsequently, to protect the 5' positions of unreacted nucleosides on the solid resin, a THF solution of acetic anhydride, 2,6-dimethylpyridine, and N-methylimidazole is supplied to container 101 through the second opening 1012. Microwaves are then applied by irradiation device 102 to cap the 5'-OH groups of the nucleosides attached to the solid resin. This step is equivalent to step (III) described above. After capping, if the first valve 103a is opened, the capping solution is discharged from container 101. The solid resin with the 5' ends protected remains on the upper surface 1051 of the first filter 105. The cleaning process using acetonitrile is repeated multiple times in the same manner as described above.

[0401] Subsequently, to oxidize the triphosphite, a mixture of, for example, iodine / pyridine / water is supplied to the container 101 through the second opening 1012. The container is then irradiated with microwaves by the irradiation device 102 to oxidize the triphosphite, thereby obtaining nucleotides. This step is equivalent to step (IV) described above. After oxidation, if the first valve 103a is opened, the oxidation solution is discharged from the container 101 through the first opening 1011. The solid resin with the nucleotides attached does not discharge but remains on the upper surface 1051 of the first filter 105. The cleaning process using acetonitrile is repeated multiple times in the same manner as described above.

[0402] To further elongate the nucleotide, the above-mentioned series of processes, including deprotection, ligation of nucleoside phosphite monoamide, protection of the unreacted 5'-terminal hydroxyl group, and oxidation, can be repeated.

[0403] To end the elongation of the nucleotide chains, and to deprotect and cleave the nucleotide chains bonded to the solid resin remaining on the upper surface 1051 of the first filter 105, concentrated ammonia can be supplied to the container 101 through the second opening 1012, and the container can be irradiated with microwaves by the irradiation device 102. The deprotected and cleaved nucleotide chains are then collected by precipitation with ethanol or similar substances and drying.

[0404] As described above, the processing apparatus 1 of Embodiment 1 is used for DNA solid-phase synthesis, thereby increasing the processing capacity of DNA synthesis through solid-phase synthesis, similar to Embodiment 1. Furthermore, the first reflective member 106 reflects microwaves passing through the first filter 105, thereby preventing microwaves from easily reaching the contents below the first filter 105 that do not contain solid-phase resin, allowing microwaves to be used efficiently for solid-phase synthesis processing.

[0405] Furthermore, in the aforementioned DNA solid-phase synthesis, it is necessary to separate the solid resin from the contents at the end of each step (I)-(IV) and leave it in container 101, drain the solvent, and further clean the solid resin remaining in container 101 with a cleaning solvent. However, by using the processing apparatus 1 of Embodiment 1 described above for solid-phase synthesis, the solid resin can be separated from the contents and left in container 101 through the first filter 105. By supplying a cleaning solvent to container 101 to clean the solid resin, or by supplying other materials, solvents, or solutions to container 101 where the solid resin has been separated, other steps of solid-phase synthesis can be performed without temporarily removing the solid resin. The solid resin separated here is, for example, a solid resin with products already bonded together.

[0406] Furthermore, in the solid-phase synthesis described above, only a portion of the multiple processes involving microwave irradiation may be performed using microwave irradiation, while other processes may not require microwave irradiation. This situation can also be considered solid-phase synthesis using microwaves.

[0407] Furthermore, the above description describes the processing apparatus 1 of Embodiment 1 as being used for solid-phase DNA synthesis via the phosphite monoamide method. However, the processing apparatus 1 of Embodiment 1 can be used for solid-phase DNA synthesis other than the phosphite monoamide method, such as the phosphotriester method or the H-phosphonate method. Even in such cases, it can achieve the same effect as the embodiment described above.

[0408] Furthermore, while the above description pertains to solid-phase DNA synthesis, the processing apparatus 1 of Embodiment 1 can also be used for solid-phase synthesis of other nucleotide chains besides DNA, and even in such cases, it can achieve the same effect as the embodiments described above. Nucleotide chains that can be solid-phase synthesized by the processing apparatus 1 of Embodiment 1 include, for example, nucleic acids such as DNA and RNA, polynucleotides, or oligonucleotides.

[0409] Furthermore, when using the processing apparatus 1 of Embodiment 1 to synthesize nucleotide chains in the solid phase, the number of nucleotides synthesized per nucleotide chain is not limited as long as there are two or more nucleotides. For example, when using the processing apparatus 1 of Embodiment 1 for DNA solid-phase synthesis, the number of deoxyribonucleotides synthesized per DNA molecule is not limited as long as there are two or more deoxyribonucleotides. For example, when using the processing apparatus 1 of Embodiment 1 for RNA solid-phase synthesis, the number of ribonucleotides synthesized per RNA molecule is not limited as long as there are two or more ribonucleotides.

[0410] Furthermore, the above description describes the case where the processing apparatus 1 of Embodiment 1 is used for solid-phase DNA synthesis, but it can be used for example... Figure 3 (a)- Figure 3 (c) The processing apparatus 1 of the modified embodiment 1 shown above, or as shown in (c) Figure 4 The processing apparatus 2 of Embodiment 2 shown above is used for solid-phase synthesis of nucleotide chains such as DNA or RNA, polynucleotides, and oligonucleotides. Even in this case, it can achieve the same effect as the various modifications of Embodiment 1 or Embodiment 2.

[0411] In addition, as Figure 3 (a)- Figure 3 (c) The processing device 1 of the modified embodiment 1 shown above, or as shown in the figure. Figure 4 When the processing apparatus 2 of Embodiment 2 shown above is used for solid-phase synthesis of nucleotide chains, for example, the description of the modified example of Embodiment 1 and the description of the solid-phase resin of Embodiment 2 above can be interpreted as a description of the solid-phase resin used in solid-phase synthesis of nucleotide chains.

[0412] Furthermore, the processing apparatus 3 and processing apparatus 4 described in Embodiments 3 and 4 can be used for the solid-phase synthesis of nucleotide chains such as DNA or RNA, polynucleotides, and oligonucleotides. In this case, the description of the solid-phase resin in Embodiments 3 and 4 can be interpreted as a description of the solid-phase resin used for solid-phase synthesis of nucleotide chains.

[0413] Furthermore, for example, when using processing apparatus 3 to perform DNA solid-phase synthesis processing via the phosphite monoamide method using the aforementioned processing apparatus 1, the solid-phase resin bonded to the hydroxyl group protected by the DMTr group at the 5' end is simply supplied to the container 301 through the third opening 1011c, and other solutions or cleaning liquids are supplied to the container 301 through the second opening 1011b. Furthermore, processing performed through the first opening 1011 can be performed solely through the first opening 1011a.

[0414] Furthermore, it can be Figure 12 (a)- Figure 12 (c) andFigure 13 (a)- Figure 13 (c) The column 5 of the modified embodiment 5 shown above. Figure 14 The column 6 of Embodiment 6 shown above is used for the solid-phase synthesis of nucleotide chains such as DNA, RNA, polynucleotides, and oligonucleotides. In this case, the description of the solid-phase resin in Embodiments 5 and 6 can be interpreted as a description of the solid-phase resin used for solid-phase synthesis of nucleotide chains. Furthermore, the contents supplied or discharged through the second opening 5011b can be supplied or discharged through tube 702b, and the contents supplied or discharged through the first opening 5011a can be supplied or discharged through tube 702a. Moreover, when using column 5 or column 6 for solid-phase synthesis of nucleotide chains, the number of nucleotides synthesized from one nucleotide chain is not limited as long as there are two or more.

[0415] Furthermore, while the embodiments described above involve solid-phase synthesis using a processing apparatus or column and employing a solid-phase resin, any solid-phase synthesis carrier suitable for solid-phase synthesis may be used, even if it is not a solid-phase resin. When using a solid-phase synthesis carrier other than a solid-phase resin to replace the solid-phase resin, for example, the description of the solid-phase resin described above can be interpreted as simply referring to a solid-phase synthesis carrier other than a solid-phase resin.

[0416] Furthermore, in the processing apparatuses 1-4 described in the above embodiments, the first openings, such as the first opening 1011 and the first opening 1011a, are described as openings for discharging the contents of the container 101. However, the first openings can also be used as openings for discharging or supplying contents, regardless of their specific use. Similarly, the second openings, such as the second opening 1012 and the second opening 1011b, are described as openings for supplying contents to the container 101. However, the second openings can also be used as openings for supplying or discharging contents, regardless of their specific use. For example, in processing devices 1 to 4, the first opening (e.g., first opening 1011 and first opening 1011a) can be used as a supply port for supplying contents into container 101, with the contents supplied from the lower end of container 101. The second opening (e.g., second opening 1012 and second opening 1011b) can be used as a discharge port for discharging the contents from container 101, with the contents discharged from the upper end of container 101. Furthermore, one or more processes performed using any of processing devices 1 to 4 can be performed by supplying contents through the first opening (e.g., first opening 1011 and first opening 1011a) and discharging contents through the second opening. Another or more processes can be performed by supplying contents through the second opening (e.g., second opening 1012 and second opening 1011b) and discharging contents through the first opening. Moreover, the supply and discharge of contents can be performed continuously or discontinuously during the processes. The term "discontinuous supply and discharge of contents" refers to a state that includes, for example, temporarily suspending the supply and discharge of contents. Discontinuous supply and discharge of contents can, for example, mean the intermittent supply and discharge of contents.

[0417] Furthermore, in cases where an openable and closable door is provided on a portion of the first end 1015a side of container 101 or container 301, where the first end 1015a side of container 101 or container 301 is removable, or where a portion of the first end 1015a side of container 101 or container 301 is detachable, or where container 101 or container 301 has other means for supplying or discharging contents from the first end 1015a side, the first opening 1011 or the first opening 1011a may be omitted. Similarly, in cases where container 101 or container 301 has other means for supplying or discharging contents from the second end 1015b side, the second opening 1012 or the second opening 1011b may be omitted. Similarly, the third opening 1011c can be omitted if the substance or liquid used in solid-phase synthesis can be supplied to the space between the first filter 105 and the second filter 107 or between the first reflective filter 205 and the second reflective filter 206 via other means. For example, in cases where the upper part or side of the container 101 or the container 301 is a detachable structure, at least one of the second opening 1012 or the second opening 1011b and the third opening 1011c can be omitted. Furthermore, for example, in the processing apparatus 3 and the processing apparatus 4, when materials used in solid-phase synthesis other than solid-phase resin are supplied to the container 301 through the third opening 1011c, the second opening 1011b can be omitted.

[0418] Furthermore, in the tubular columns 5 and 6 described in the above embodiments, the first opening 5012a of the first end 5011a inside the cylindrical member 501 is used as a discharge port. However, the first opening 5012a of the first end 5011a can be used as a discharge port for discharging contents or as a supply port for supplying contents, regardless of its use. Similarly, the second end 5011b can be used as a supply port or a discharge port. For example, the first opening 5012a of the first end 5011a of the cylindrical member 501 can be used as a supply port, and the second opening 5012b of the second end 5011b can be used as a discharge port. Furthermore, one or more processes using tubing 5 or tubing 6 can be performed by supplying contents through the second opening 5012b of the second end 5011b of the cylindrical member 501 and discharging contents through the first opening 5012a of the first end 5011a. Another one or more processes besides this can be performed by supplying contents through the first opening 5012a of the first end 5011a and discharging contents through the second opening 5012b of the second end 5011b. In addition, the supply and discharge of contents can be continuous or discontinuous. Discontinuous supply and discharge of contents refers to, for example, a state that includes temporarily stopping the supply and discharge of contents. Discontinuous supply and discharge of contents can, for example, be intermittent supply and discharge of contents.

[0419] Furthermore, in the processing apparatus or tubing of the above embodiments, contents can flow between a first opening (e.g., first opening 1011, first opening 1011a, or first opening 5012a) and a second opening (e.g., second opening 1012, second opening 1011b, or second opening 5012b) within the container (e.g., within container 101, container 301, or cylindrical member 501). For example, contents are continuously supplied to the container through the second opening, while simultaneously the contents are continuously discharged from the container through the first opening, causing the contents to flow from the second opening side to the first opening side within the container. Moreover, particularly in processing apparatus 3, processing apparatus 4, tubing 5, and tubing 6, contents can be continuously supplied to the container through the first opening, while simultaneously the contents are continuously discharged from the container through the second opening, causing the contents to flow from the first opening side to the second opening side within the container. At this time, for example, the second filter 107, the second reflective filter 206, the second filter 504, or the second reflective filter 605 are filters to prevent solids such as solid resin in the container 301 or the cylindrical member 501 from being discharged along with the contents. Furthermore, the flow direction within the container can be any of the above. Also, the flow direction within the container can be changed during processing or in conjunction with the steps constituting the processing.

[0420] Furthermore, in the processing apparatus or column described above, microwaves can be irradiated into the container while the contents are continuously flowing within the container (e.g., container 301 or cylindrical member 501). For example, one or more processes can be performed simultaneously with microwave irradiation, including processes performed using any of the processing apparatus 1 to 4, column 5, or column 6, while the contents are continuously flowing within the container. For example, microwaves can be irradiated while the contents are continuously flowing within the container from the first opening side to the second opening side. For example, the processing apparatus or column can be used as a so-called flowing processing apparatus or flowing column for processing the contents flowing within the container.

[0421] Furthermore, while the contents flow from the first opening side to the second opening side within the container, one or more of the multiple processes performed using the processing apparatus or column described in the above embodiments can be carried out. Additionally, while the contents flow from the second opening side to the first opening side within the container, one or more other processes besides the one or more described processes can be carried out. Moreover, one or more of the multiple processes can be one or more steps among the multiple steps included in a single process.

[0422] Furthermore, in embodiments 3 and 4 described above, the processing apparatus 3 and processing apparatus 4 are described as having a first filter 105 and a first reflective member 106, and a second filter 107 and a second reflective member 108. However, only one of the first filter 105 and the first reflective member 106, and the second filter 107 and the second reflective member 108 may be used. This is also the same when using a first reflective filter 205 that integrates the first filter 105 and the first reflective member 106, and a second reflective filter 206 that integrates the second filter 107 and the second reflective member 108.

[0423] Furthermore, in embodiments 1 and 2 described above, the case where the first end 1015a is the lower end of the container 101 and the second end 1015b is the upper end of the container 101 is used as an example for explanation. However, it is also possible for the first end 1015a to be the upper end of the container 101 and the second end 1015b to be the lower end of the container 101. Also, the first end 1015a and the second end 1015b do not have to be either the upper or lower end of the container 101. For example, the first end 1015a and the second end 1015b can be the two ends of the container 101 in a generally horizontal direction. This is also the same as in the container 301 of embodiments 3 and 4 described above, or the cylindrical member 501 of embodiments 5 and 6 described above.

[0424] Furthermore, while embodiments 1 and 2 describe a case where container 101 is a longitudinal container, the shape of container 101 can be arbitrary, for example, it can be a transverse container. A transverse container is, for example, a container whose length direction is transverse. When container 101 is a transverse container, the first end 1015a and the second end 1015b of container 101 are the two ends in the transverse direction along the length of container 101. In addition, when container 101 is, for example, a transverse container, a first filter 105 and a first reflective member 106 are provided between the irradiation position and the first end 1015a of container 101, and a second filter 107 and a second reflective member 108 are provided between the irradiation position and the second end 1015b of container 101. This allows the presence area of ​​solid matter, such as solid resin, in the contents to be set without changing the size of container 101, so that most of the irradiated microwaves can be irradiated to this area. Therefore, appropriate microwave irradiation can be performed according to the amount of solid resin, etc. Furthermore, when container 101 is a horizontal container, its length direction can be tilted to approximately 30 degrees relative to the horizontal direction. For example, a platform (not shown) or container 101 can be arranged in an tilted state, and a container holding device such as a hanging device that suspends the tilted container 101 can also be provided. Moreover, this is also the same as the container 301 in embodiments 3 and 4 described above.

[0425] Furthermore, while embodiments 5 and 6 describe the use of the tubular column with the axial direction of the cylindrical member 501 as the vertical direction, the tubular column can also be used with the axial direction of the cylindrical member 501 as the horizontal direction. Additionally, the tubular column can be used with the axial direction inclined. For example, it can be used with the axial direction inclined at approximately 30 degrees relative to the horizontal direction. For example, it can have a mounting platform (not shown) or a hanging device in an inclined state, or a holding device, etc., to hold the tubular member 501 in an inclined state.

[0426] Furthermore, the containers 101 and 301 in the above embodiments can be composed of multiple components. For example, in embodiments 3 and 4, when the container 301 is a vertical container 101 and the second end 1015b is the upper end of the container 101, the container 101 can be constructed by a first component (not shown) with an opening at the top and a first end at the bottom, and a second component (not shown) connected to the opening portion at the top of the first component and having a second opening portion 1011b at the top. In this case, the second component can be a pipe-like component connected to the upper part of the first component. Also, in this case, the second filter 107 and the second reflective component 108 can be disposed on the upper side of the first component, and one of them can be disposed in a manner that blocks the opening portion at the top of the first component.

[0427] This invention is not limited to the above embodiments and can be modified in various ways. All of these modifications are included within the scope of this invention, which need not be elaborated further.

[0428] Industrial utilization

[0429] As described above, the processing apparatus of the present invention is suitable for use as an apparatus for processing performed by irradiation with microwaves, and in particular for use as an apparatus for processing including a step of filtering solids.

Claims

1. A processing apparatus, characterized in that, A processing apparatus for solid-phase synthesis, comprising: A container, which is made of a microwave-reflective material and has a first end and an emitting part for microwaves irradiating into the interior; A first filter, configured to separate the container, separates solids from the contents of the container; and A first reflective member is disposed on the side closer to the first end of the emission portion, separating the container, and allows contents that have passed through at least the first filter to pass through, while reflecting microwaves so that microwaves do not easily irradiate the contents that have already passed through. The first reflective member has a plurality of holes or openings through which at least one or more contents can pass. The diameter of each hole or opening is smaller than half the wavelength of a microwave. The solid material is a carrier used in solid-phase synthesis.

2. The processing apparatus according to claim 1, characterized in that, The first reflective member is provided on the side closer to the first end of the first filter or between the first filter and the emission portion.

3. The processing apparatus according to claim 1, characterized in that, The first filter and the first reflective member are configured in an overlapping manner.

4. The processing apparatus according to claim 1, characterized in that, The first reflective member is disposed between the first filter and the emission portion, and allows the solid to pass through.

5. The processing apparatus according to claim 1, characterized in that, The first filter is integrated with the first reflective component to form a first reflective filter, which is a filter that separates solid matter from the contents of the container and reflects microwaves.

6. The processing apparatus according to claim 1, characterized in that, The container further has a second end. The ejector portion is located between the first end and the second end of the container. The processing apparatus further comprises: A second filter, disposed at a second end relative to the first filter and the first reflective member in a manner that separates the container, separates solids from the contents of the container; and The second reflective member is disposed on the side closer to the second end than the first filter and the emission portion, in a manner that separates the container, and allows the contents of at least the second filter to pass through, while reflecting microwaves.

7. The processing apparatus according to claim 6, characterized in that, The second reflective member is provided on the side closer to the second end of the second filter or between the second filter and the emission portion.

8. The processing apparatus according to claim 6, characterized in that, The second filter is configured to overlap with the second reflective member.

9. The processing apparatus according to claim 6, characterized in that, The second reflective member is disposed between the second filter and the emission portion, and allows the solid to pass through.

10. The processing apparatus according to claim 6, characterized in that, The second filter is integrated with the second reflective member to form a second reflective filter, which is a filter that separates solid matter from the contents of the container and reflects microwaves.

11. The processing apparatus according to claim 1, characterized in that, A first opening is provided on the container at the first end side relative to the first filter and the first reflector, and the first opening is used for at least one of supplying and discharging contents.

12. The processing apparatus according to claim 6, characterized in that, A first opening is provided on the side of the container closer to the first end than the first filter and the first reflector, the first opening being used for at least one of supplying and discharging contents, and a second opening is provided on the side of the container closer to the second end than the second filter and the second reflector, the second opening being used for at least one of supplying and discharging contents.

13. The processing apparatus according to claim 11, characterized in that, The first end is the lower end of the container. The first opening is a discharge port for discharging contents.

14. The processing apparatus according to claim 12, characterized in that, While the contents are flowing within the container between the first opening and the second opening, microwave irradiation is performed from the exit section.

15. The processing apparatus according to claim 12, characterized in that, conduct: The process of supplying contents through the first opening and discharging contents through the second opening, and the processing performed within the container; and The contents are supplied through the second opening and discharged through the first opening, and the processing takes place within the container.

16. The processing apparatus according to claim 6, characterized in that, The container is a cylindrical member having an opening at a first end and an opening at a second end. The ejection portion of the container is located on the side of the cylindrical member. The first filter is configured to block the first end side of the cylindrical member. The first reflective member is configured to block the first end side of the cylindrical member. The second filter is configured to block the second end side of the cylindrical member. The second reflective member is configured to block the second end side of the cylindrical member.

17. The processing apparatus according to claim 16, characterized in that, The processing device is a tubular column.

18. The processing apparatus according to any one of claims 1 to 17, characterized in that, The solid-phase synthesis is the synthesis of peptide or nucleotide chains that are already bonded to a solid-phase synthesis carrier.

19. The processing apparatus according to any one of claims 1 to 17, characterized in that, The processing device is a processing device that performs microwave irradiation in multiple modes.

20. The processing apparatus according to any one of claims 1 to 17, characterized in that, The processing apparatus further includes an irradiation device for irradiating microwaves into the container by the emission section.

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