A shear stress generating device for constructing a rat coccygeal disc degeneration model
By designing a shear loading device including a fixing device and a pressurization device, the problem of single axial compression load in the body animal experiment in the prior art is solved, and a reliable shear stress is generated on the rat tail is achieved, ensuring that The accuracy and safety of the experiment.
Patent Information
- Application Number
- CN202010167388.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2020-03-11
- Publication Date
- 2024-12-31
- Estimated Expiration
- 2040-03-11
AI Technical Summary
The prior art studies axial compression loads in bulk animal experiments with excessive single study, lacking devices that can generate shear stress, making it difficult to establish an effective model of rat tail disc degeneration.
A shear loading device including a first fixing device and a second fixing device is designed, and a shear stress is formed by parallel arrangement of the first and second bone needles, the coupling of the pressing spring and the adjustment bolt, and Equipped with a rat-tail clamping device to ensure stable fixation and safety of the device, and a pressure sensor is used to detect shear loading force.
Reliable shear stress is achieved on the rat tail, reducing damage to the tailbone, ensuring the accuracy and safety of the experiment, and avoiding inaccurate load capacity estimation caused by wear and accuracy problems of the pressurized device.
Smart Images
Figure CN111227983B_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to an experimental device, more specifically to a rat tail shear force pressurizer for constructing an intervertebral disc degeneration model. Background Art
[0002] The intervertebral disc is an important part of the spine. Its main function is to absorb, cushion and transfer the load on the human body. At the same time, its existence allows the spine to move within a certain range. However, the degeneration of the intervertebral disc often causes dysfunction or even loss of spinal function. Some degenerated intervertebral discs can also cause spinal degenerative diseases such as lumbar disc herniation, discogenic low back pain and cervical spondylosis, which seriously affect the quality of life of patients and impose a huge economic burden on families and society. Therefore, it is urgently needed to conduct research on related diseases from the perspective of intervertebral disc degeneration.
[0003] In order to further clarify the relationship between load and intervertebral disc degeneration, researchers have successively established various in vivo animal models. So far, more than 10 animals have been used to establish intervertebral disc degeneration models, including mice, rabbits, dogs, cattle, sheep, pigs, monkeys, etc. As early as the 1990s, researchers installed loading devices on the tail vertebrae of experimental animals such as mice to achieve loading experimental research on intervertebral discs; there are also corresponding installation devices, such as the "Mouse Tail Static Intervertebral Disc Pressurization Device" disclosed in the Chinese patent number 201520545337.0, and the "Mouse Intervertebral Disc Degeneration Model Static Pressurization Device" disclosed in the Chinese patent number 201620605710.1. However, the above-mentioned loading devices are only used for axial loading force, and there is an excessive single study of axial compression load in in vivo animal experimental research. In order to make up for the deficiency of the previous in vivo animal experimental research that only studied the axial compression load, some researchers have established a shear stress-induced disc degeneration model on rabbits, such as the "Experimental Animal Rabbit Lumbar Disc Shear Stress Applicator" disclosed in the patent document with Chinese patent number 201210438551.7, but the economic efficiency of rabbits is obviously lower than that of mice. Therefore, it is necessary to design a shear force loading device that can be applied to mice and can generate shear stress, so as to establish a shear force-induced disc degeneration model on mice. Summary of the invention
[0004] The purpose of the utility model is to overcome the defects of the prior art. The utility model provides a shear stress generating device which can be fixed on the rat's tail to provide shear force for constructing a rat's tail intervertebral disc degeneration model.
[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0006] A shear stress generating device for constructing a rat coccygeal intervertebral disc degeneration model comprises a first fixing device and a second fixing device respectively fixed on two adjacent rat coccygeal sections, and a shear force loading device which associates the first fixing device and the second fixing device to form a shear pressure, characterized in that: the first fixing device comprises a first fixing ring, the first fixing ring is provided with a first bone needle corresponding to the diameter of the first fixing ring, the second fixing device comprises a second fixing ring, the second fixing ring is provided with a second bone needle corresponding to the diameter of the second fixing ring, the first bone needle is parallel to the second bone needle, a pair of oppositely arranged positioning seats are axially extended from the circumferential edge of the first fixing ring, the second fixing ring is located between the two positioning seats, the shear force loading device comprises a pressure spring arranged on one of the positioning seats, the pressure spring is pressed between the second fixing ring and the positioning seat to form an elastic top pressure, the direction of the elastic top pressure is perpendicular to the extension direction of the first bone needle, and an adjusting bolt is threadedly matched on the other positioning seat, and the adjusting bolt is pressed against the second fixing ring under the elastic top pressure of the pressure spring.
[0007] By adopting the above technical solution, the adjusting bolt is operated so that the first fixing ring and the second fixing ring are in a coaxial position. Since the first bone needle is parallel to the second bone needle and has corresponding diameters, the first bone needle and the second bone needle are coaxial, and the coccyx fixed by the first bone needle and the coccyx fixed by the second bone needle are in a non-pressurized state. After that, the adjusting bolt is operated, and under the action of the pressurized spring, the second fixing ring is radially displaced, and shear stress is formed on two adjacent coccyx sections fixed by the first fixing ring and the second fixing ring; the shear stress is perpendicular to the first fixed bone (and the second bone needle), which can ensure that reliable shear stress is formed on the coccyx and reduce damage to the coccyx.
[0008] Preferably, the first fixing ring is detachably equipped with a rat tail clamping device for being put on the rat tail, the first fixing ring is provided with a first guide pin hole for the first bone needle to be inserted, and the second fixing ring is provided with a second guide pin hole for the second bone needle to be inserted. Under this structural design, the rat tail clamping device realizes the positioning of the rat tail shear force pressurizer on the rat tail, and cooperates with the first guide pin hole and the second guide pin hole to realize the guided insertion of the first bone needle and the second bone needle. The first bone needle and the second bone needle are accurately and reliably inserted into the rat coccyx, and the rat tail blood vessels can be effectively avoided, and the coaxiality of the first bone needle and the second bone needle during insertion is ensured; the rat tail clamping device is disassembled after the first bone needle and the second bone needle are inserted, so as to avoid the interference of the rat tail clamping device with the shear force pressurizer.
[0009] Preferably, the second fixing ring has a positioning support surface that contacts the adjacent positioning seat when the first fixing ring and the second fixing ring are coaxial, and the corresponding positioning seat is provided with a positioning surface that corresponds to the positioning support surface. Under this structural design, when the shear force loader is in use, the adjusting bolt is adjusted to drive the second fixing ring so that the positioning support surface of the second fixing ring contacts the positioning surface of the positioning seat, so that the first fixing ring and the second fixing ring are coaxial, and the second fixing ring is returned to the initial position, at which time the pressure spring stores energy; it is convenient to adjust the initial position of the second fixing ring, and when the second fixing ring is in the initial position, the adjusting bolt and the pressure spring can clamp and fix the second fixing ring, which is convenient for the introduction of the second bone needle.
[0010] Preferably, the rat tail clamping device includes rat tail clamps respectively located on both axial sides of the first fixed ring, each rat tail clamp includes two groups of clamp ring assemblies arranged oppositely, and the two groups of clamp ring assemblies of each rat tail clamp include a clamp seat, a clamp ring and a clamping spring, the clamp seat is detachably fixed on the first fixed ring, a sliding shaft is fixed on the clamp ring, the sliding shaft axially slides on the clamp seat, the clamping spring is sleeved on the sliding shaft, one end of the clamping spring abuts on the clamp ring, and the other end of the clamping spring abuts on the clamp seat, the clamp rings of the two groups of clamp ring assemblies are arranged oppositely, and the clamping spring pushes the corresponding clamp ring to approach the other clamp ring oppositely, thereby forming an elastic rat tail clamping hole through two opposite clamp rings. Under this structural design, the rat tail clamp realizes elastic clamping and fixing of the rat tail, and the clamping is reliable. The rat tail clamps are installed on both axial sides of the first fixed ring to realize reliable fixing of the shear force pressurizer on the rat tail.
[0011] Preferably, both of the two positioning seats are provided with axially through-going connection holes, and the two groups of clamp ring assemblies of the two rat tail clamps are arranged opposite to each other along the axial position of the first fixed ring, and a connecting shaft is fixed on one clamp seat of the two groups of clamp ring assemblies corresponding to the axial position, and the connecting shaft passes through the connecting hole, and the other clamp seat of the two groups of clamp ring assemblies corresponding to the axial position is provided with a through hole sleeved on the connecting shaft, and a connecting thread is provided at one end of the through hole of the clamp seat through which the connecting shaft passes, and a locking nut is threadedly screwed on the connecting thread of the connecting shaft, and the clamp seats of the two groups of clamp ring assemblies corresponding to the axial position are clamped and fixed to the first fixed ring through the connecting shaft and the locking nut. Under this structural design, the two groups of clamp ring assemblies of the two rat tail clamps are clamped and fixed to the first fixed ring, which has the advantages of simple structure and convenient assembly and disassembly operation, and the positioning seat on the first fixed ring is effectively used to realize the installation of the clamp ring assemblies on both axial ends of the first fixed ring, which has the advantage of compact structure.
[0012] Preferably, the inner hole edges at both axial ends of the clamp ring have rounded corners. With this structural design, damage to the rat tail is avoided, and it is convenient to directly pull out the clamp seat axially to remove the rat tail clamp.
[0013] Preferably, a pressure sensor is embedded in the positioning seat that abuts against the pressure spring, and the pressure spring abuts against a top pressure seat, which includes a conical spring positioning cone, and the bottom surface of the spring positioning cone has a contact protrusion. The pressure spring is sleeved on the spring positioning cone, and the top pressure seat abuts against the pressure sensor through the contact protrusion. Under this structural design, a pressure sensor is used to detect the shear loading force, and the shear loading force can be obtained more intuitively and reliably, which can avoid the problem of inaccurate loading force estimation caused by wear and precision problems of the pressure spring and the adjusting bolt, and the structural design of the top pressure seat can ensure that the spring force of the pressure spring is accurately and reliably reflected on the pressure sensor.
[0014] The utility model is further described below in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 A schematic diagram of the structure of a shear stress generating device for constructing a rat coccygeal intervertebral disc degeneration model according to a specific embodiment of the utility model;
[0016] Figure 2 An exploded view of a shear stress generating device for constructing a rat coccygeal intervertebral disc degeneration model according to a specific embodiment of the utility model;
[0017] Figure 3 This is a schematic structural diagram of a shear stress generating device for constructing a rat tail intervertebral disc degeneration model according to a specific embodiment of the utility model after removing the rat tail clamping device. DETAILED DESCRIPTION
[0018] See attached Figures 1 to 3 The utility model discloses a shear stress generating device for constructing a rat coccygeal intervertebral disc degeneration model, comprising a first fixing device 1 and a second fixing device 2 respectively fixed on two adjacent rat coccyxes, and a shear force loading device associated with the first fixing device 1 and the second fixing device 2 to form a shear pressure. The first fixing device 1 comprises a first fixing ring 11, on which a first bone needle 12 corresponding to the diameter of the first fixing ring 11 is disposed, the second fixing device comprises a second fixing ring 21, on which a second bone needle 22 corresponding to the diameter of the second fixing ring 21 is disposed, and the first bone needle 12 and the second bone needle 22 are associated with each other. 22 are parallel, a pair of relatively arranged positioning seats 111, 112 are axially extended from the circumferential edge of the first fixing ring 11, and the second fixing ring 21 is located between the two positioning seats 111, 112. The shear force loading device includes a pressure spring 31 arranged on one of the positioning seats 111, and the pressure spring 31 is pressed between the second fixing ring 21 and the positioning seat 111 to form an elastic top pressure, and the direction of the elastic top pressure is perpendicular to the extension direction of the first bone needle 12. An adjusting bolt 32 is threadedly fitted on the other positioning seat 112, and the adjusting bolt 32 is pressed against the second fixing ring 21 under the elastic top pressure of the pressure spring 31.
[0019] The first fixing ring 11 is detachably equipped with a rat tail clamping device for being put on the rat tail. The first fixing ring 11 is provided with a first guide pin hole 113 for the first bone needle 12 to pass through, and the second fixing ring 21 is provided with a second guide pin hole 211 for the second bone needle 22 to pass through. The rat tail clamping device realizes the positioning of the rat tail shear force pressurizer on the rat tail, and cooperates with the first guide pin hole and the second guide pin hole to realize the guided insertion of the first bone needle and the second bone needle. The first bone needle and the second bone needle are accurately and reliably inserted into the rat coccyx, and can effectively avoid the rat tail blood vessels, and ensure the coaxiality of the first bone needle and the second bone needle when inserted; the rat tail clamping device is disassembled after the first bone needle and the second bone needle are inserted to avoid the interference of the rat tail clamping device with the shear force pressurizer. The first bone needle and the second bone needle can be locked on the first fixing ring and the second fixing ring by locking screws; or they can be fixed on the first fixing ring and the second fixing ring by bending operation.
[0020] Furthermore, the second fixing ring 21 has a positioning support surface 212 that contacts the adjacent positioning seat 112 when the first fixing ring 11 and the second fixing ring 21 are coaxial, and the corresponding positioning seat 112 is provided with a positioning surface 1121 that corresponds to the positioning support surface 212. When the shear force loader is in use, the adjusting bolt is adjusted to drive the second fixing ring 21 so that the positioning support surface of the second fixing ring 21 contacts the positioning surface of the positioning seat, so that the first fixing ring and the second fixing ring are coaxial, and the second fixing ring is returned to the initial position, at which time the pressure spring stores energy; the initial position adjustment of the second fixing ring 21 is convenient, and when the second fixing ring 21 is in the initial position, the adjusting bolt and the pressure spring clamp and fix the second fixing ring, so as to facilitate the introduction of the second bone needle.
[0021] The rat tail clamping device includes rat tail clamps respectively located on both axial sides of the first fixed ring 11, each rat tail clamp includes two groups of clamp ring assemblies 41 arranged oppositely, and the two groups of clamp ring assemblies 41 of each rat tail clamp include a clamp seat 411, a clamp ring 412 and a clamping spring 413. The clamp seat 411 is detachably fixed on the first fixed ring 11, a sliding shaft 414 is fixed on the clamp ring 412, and the sliding shaft 414 is axially slidably fitted on the clamp seat 411. The clamping spring 413 is sleeved on the sliding shaft 414, one end of the clamping spring 413 abuts on the clamp ring 412, and the other end of the clamping spring 413 abuts on the clamp seat 411. The clamp rings 412 of the two groups of clamp ring assemblies 41 are arranged oppositely, and the clamping spring 413 pushes the corresponding clamp ring 412 to approach the other clamp ring 412 oppositely, thereby forming an elastic rat tail clamping hole 40 through the two opposite clamp rings 412. The rat tail clamp realizes elastic clamping and fixing of the rat tail, and the clamping is reliable. The rat tail clamp is installed on both axial sides of the first fixing ring to realize reliable fixing of the shear force pressurizer on the rat tail.
[0022] The two positioning seats 111, 112 are both provided with an axially penetrating connecting hole 114, and the two groups of clamp ring assemblies 41 of the two rat tail clamps are arranged opposite to each other along the axial position of the first fixing ring 11. A connecting shaft 4111 is fixed on one clamp seat 411 of the two groups of clamp ring assemblies 41 corresponding to the axial position, and the connecting shaft 4111 passes through the connecting hole 114, and the other clamp seat 411 of the two groups of clamp ring assemblies 41 corresponding to the axial position is provided with a through hole 4112 sleeved on the connecting shaft 4111, and the connecting shaft 4111 passes through the through hole 4112 of the clamp seat 411 at one end thereof. A locking nut 4113 is threadedly screwed on the connecting thread of the connecting shaft 4111, and the locking nut 4113 is designed as a hand-tightening nut. The clamp seats 411 of the two groups of clamp ring assemblies 41 corresponding to the axial position are fixed to the first fixing ring 11 by being locked and clamped via the connecting shaft 4111 and the locking nut 4113. The two groups of clamp ring assemblies of the two rat tail clamps are clamped and fixed on the first fixing ring, which has the advantages of simple structure and convenient disassembly and assembly. The positioning seat on the first fixing ring is effectively used to install the clamp ring assembly on both ends of the first fixing ring in the axial direction, which has the advantage of compact structure. A finger ring 4141 is provided at the top of the sliding shaft 414. When disassembling, a finger can be inserted into the finger ring to pull the sliding shaft, so as to facilitate safer and more convenient disassembly of the clamp ring assembly. The inner hole edges of the clamp ring 412 at both ends of the axial direction have chamfered corners 4121. It is convenient to directly pull the clamp seat axially to disassemble the rat tail clamp, avoiding damage to the rat tail.
[0023] The loading force estimation of the pressure spring and the adjusting bolt is inaccurate due to wear and precision problems, and the pressure spring has fatigue problems after repeated use. To solve the above problems, a pressure sensor 5 is embedded in the positioning seat 112 abutting against the pressure spring 31, and the pressure spring 31 abuts against a top pressure seat 33. The top pressure seat 33 includes a conical spring positioning cone 331, and the bottom surface of the spring positioning cone 331 is protruded with a contact protrusion 332. The pressure spring 31 is sleeved on the spring positioning cone 331, and the top pressure seat 33 abuts against the pressure sensor 5 through the contact protrusion 332. The pressure sensor is used to detect the shear loading force, and the shear loading force can be obtained more intuitively and reliably. The structural design of the top pressure seat can ensure that the spring force of the pressure spring is accurately and reliably reflected on the pressure sensor.
[0024] When in use, operate the adjusting bolt 32 to place the second fixing ring 21 in the initial position, put the device on the mouse tail, and clamp the mouse tail clamping device on the mouse tail to achieve the positioning of the entire device; insert the first bone needle 12 and the second bone needle 22 through the corresponding first guide needle hole and the second guide needle hole. Since the first bone needle 12 is parallel to the second bone needle 22 and has corresponding diameters, the first bone needle 12 and the second bone needle 22 are coaxial, and the coccyx fixed by the first bone needle 12 and the coccyx fixed by the second bone needle 22 are in a non-pressurized state. Then operate the adjusting bolt, and under the action of the pressure spring 31, the second fixing ring 21 is displaced radially, and shear stress is formed on the two adjacent coccyx fixed by the first fixing ring 11 and the second fixing ring 21; the shear stress is perpendicular to the first bone needle (and the second bone needle), which can ensure that reliable shear stress is formed on the coccyx and reduce damage to the coccyx.
Claims
1. A shear stress generating device for constructing a rat coccygeal intervertebral disc degeneration model, comprising a first fixing device and a second fixing device respectively fixed on two adjacent coccygeal sections of the rat, and a shear force loading device associated with the first fixing device and the second fixing device to form a shear pressure, characterized in that: The first fixing device comprises a first fixing ring, on which a first bone needle corresponding to the diameter of the first fixing ring is disposed, and the second fixing device comprises a second fixing ring, on which a second bone needle corresponding to the diameter of the second fixing ring is disposed, the first bone needle is parallel to the second bone needle, a pair of relatively arranged positioning seats are axially extended from the circumferential edge of the first fixing ring, and the second fixing ring is located between the two positioning seats, and the shear force loading device comprises a pressure spring disposed on one of the positioning seats, the pressure spring presses between the second fixing ring and the positioning seat to form an elastic top pressure, the direction of the elastic top pressure is perpendicular to the extension direction of the first bone needle, and the other positioning seat is located between the two positioning seats. The seat is threaded with an adjusting bolt, and the adjusting bolt is pressed against the second fixing ring under the elastic top pressure of the pressure spring; the first fixing ring is detachably equipped with a rat tail clamping device for being sleeved on the rat tail, the first fixing ring is provided with a first guide needle hole for the first bone needle to be inserted, and the second fixing ring is provided with a second guide needle hole for the second bone needle to be inserted; a pressure sensor is embedded in the positioning seat abutting the pressure spring, the pressure spring is abutted against a top pressure seat, the top pressure seat includes a conical spring positioning cone, the bottom surface of the spring positioning cone is protruded with a contact protrusion, the pressure spring is sleeved on the spring positioning cone, and the top pressure seat abuts against the pressure sensor through the contact protrusion.
2. The shear stress generating device for constructing a rat coccygeal disc degeneration model according to claim 1, characterized in that: The second fixing ring has a positioning support surface that contacts the adjacent positioning seat when the first fixing ring and the second fixing ring are coaxial, and the corresponding positioning seat is provided with a positioning surface that corresponds to the positioning support surface.
3. The shear stress generating device for constructing a rat coccygeal disc degeneration model according to claim 1, characterized in that: The rat tail clamping device includes rat tail clamps respectively located on both axial sides of the first fixed ring, each rat tail clamp includes two groups of clamp ring assemblies arranged oppositely, and the two groups of clamp ring assemblies of each rat tail clamp include a clamp seat, a clamp ring and a clamping spring. The clamp seat is detachably fixed on the first fixed ring, a sliding shaft is fixed on the clamp ring, and the sliding shaft is axially slidably fitted on the clamp seat. The clamping spring is sleeved on the sliding shaft, one end of the clamping spring abuts on the clamp ring, and the other end of the clamping spring abuts on the clamp seat. The clamp rings of the two groups of clamp ring assemblies are arranged oppositely, and the clamping spring pushes the corresponding clamp ring to approach the other clamp ring oppositely, thereby forming an elastic rat tail clamping hole through the two opposite clamp rings.
4. The shear stress generating device for constructing a rat coccygeal disc degeneration model according to claim 3, characterized in that: The two positioning seats are each provided with an axially through-going connecting hole. The two groups of clamp ring assemblies of the two rat tail clamps are arranged opposite to each other along the axial position of the first fixed ring. A connecting shaft is fixed on one of the clamp seats in the two groups of clamp ring assemblies corresponding to the axial positions, and the connecting shaft passes through the connecting hole. The other clamp seat in the two groups of clamp ring assemblies corresponding to the axial positions is provided with a through hole sleeved on the connecting shaft. A connecting thread is provided at one end of the connecting shaft passing through the through hole of the clamp seat. A locking nut is threadedly installed on the connecting thread of the connecting shaft. The clamp seats of the two groups of clamp ring assemblies corresponding to the axial positions are fixed to the first fixed ring by being clamped and fixed via the connecting shaft and the locking nut.
5. The shear stress generating device for constructing a rat coccygeal disc degeneration model according to claim 4, characterized in that: The inner hole edges at both axial ends of the clamp ring are chamfered.