Liquid adding device, analyzer, and liquid adding method

Abstract

The application provides a liquid adding device, an analyzer and a liquid adding method. The liquid adding device comprises a first electromagnetic valve, a second electromagnetic valve, a first pipeline, a second pipeline, a third pipeline, a fourth pipeline and a suction device. The first pipeline is connected with the first electromagnetic valve. The second pipeline is connected with the second electromagnetic valve. The third pipeline connects the first electromagnetic valve and the second electromagnetic valve. The fourth pipeline connects the first electromagnetic valve and the suction device. The suction device is used for: sucking a first reagent through the fourth pipeline, the first electromagnetic valve and the first pipeline; sucking a second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve and the second pipeline; pushing out the first reagent and / or the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline and the second electromagnetic valve; and buffering the second reagent in the third pipeline when the second reagent is sucked. The second reagent does not enter the suction device, so that the air hole phenomenon of the second reagent in the suction device is avoided, air bubbles are generated, and the reagent adding precision is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical detection analysis, and particularly relates to a liquid adding device, an analyzer and a liquid adding method. BACKGROUND

[0002] Blood cell analyzers have been widely used, and the reliability of the performance of the instrument directly affects the experience of the user and the accuracy of the measurement result, so the reliability of the instrument is improved from various aspects during the research and development process.

[0003] At present, a blood cell analyzer uses several reagents, and the power required for adding the reagents from the reagent bottle to the reaction pool is usually provided by a syringe or a quantitative pump.

[0004] After the instrument runs a certain amount of samples, some bubbles can accumulate in the reagent pipeline, and when the bubbles reach a certain amount, the accuracy of the instrument in adding reagents is reduced, thereby affecting the stability of the instrument.

[0005] The main reason for the generation of bubbles is the formation of a cavity phenomenon in the process of reagent entering the syringe from the reagent pipeline.

[0006] Common methods for processing bubbles include:

[0007] (1) Discarding reagents to expel bubbles;

[0008] (2) Increasing a bubble filter / catcher.

[0009] The above processing methods are to process bubbles after the bubbles are generated.

[0010] Method (1) increases the reagent consumption, which is difficult for users to accept for relatively expensive reagents;

[0011] Method (2) increases the design cost of the instrument, and some macromolecular reagents (such as CRP specific protein reagent R2 liquid) are not suitable for bubble filters / catchers. SUMMARY

[0012] The present application provides a liquid adding device, an analyzer and a liquid adding method to reduce the problem of bubbles affecting the accuracy of adding reagents in the prior art.

[0013] To solve the above technical problems, one technical scheme adopted by the present application is to provide a liquid adding device, which comprises a first electromagnetic valve, a second electromagnetic valve, a first pipeline, a second pipeline, a third pipeline, a fourth pipeline and a suction device.

[0014] The first pipeline is connected with the first electromagnetic valve.

[0015] The second pipeline is connected with the second electromagnetic valve.

[0016] The third pipeline connects the first electromagnetic valve and the second electromagnetic valve;

[0017] The fourth pipeline connects the first electromagnetic valve and the suction device;

[0018] The suction device is used for:

[0019] sucking the first reagent through the fourth pipeline, the first electromagnetic valve and the first pipeline;

[0020] sucking the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve and the second pipeline;

[0021] pushing out the first reagent and / or the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline and the second electromagnetic valve;

[0022] Wherein, when the second reagent is sucked, the second reagent is buffered in the third pipeline.

[0023] According to an embodiment of the present application, the first electromagnetic valve has a first main channel, a first branch channel and a second branch channel in selective communication with the first main channel;

[0024] The second electromagnetic valve has a second main channel, a third branch channel and a fourth branch channel in selective communication with the second main channel;

[0025] The first main channel is connected to one end of the fourth pipeline;

[0026] The first branch channel is connected to one end of the first pipeline;

[0027] The second branch channel is connected to one end of the third pipeline;

[0028] The second main channel is connected to the other end of the third pipeline;

[0029] The third branch channel is connected to one end of the second pipeline;

[0030] The fourth branch channel serves as a liquid outlet.

[0031] According to an embodiment of the present application, the liquid adding device further comprises a fifth pipeline connected to the fourth branch channel.

[0032] According to an embodiment of the present application, the liquid adding device further comprises a reaction pool, which is arranged at the liquid outlet end of the fourth branch channel or the liquid outlet end of the fifth pipeline.

[0033] According to an embodiment of the present application, the suction device is a syringe or a quantitative pump.

[0034] According to an embodiment of the present application, the liquid adding device further comprises a stepper motor, and the stepper motor is configured to drive the syringe to move quantitatively.

[0035] According to an embodiment of the present application, the first reagent is drawn through the fourth pipeline, the first electromagnetic valve, and the first pipeline.

[0036] The first main channel is communicated with the first sub-channel, and the second main channel is communicated with the fourth sub-channel.

[0037] According to an embodiment of the present application, the second reagent is drawn through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve, and the second pipeline.

[0038] The first main channel is communicated with the second sub-channel, and the second main channel is communicated with the third sub-channel.

[0039] According to an embodiment of the present application, the first reagent and / or the second reagent is pushed out through the fourth pipeline, the first electromagnetic valve, the third pipeline, and the second electromagnetic valve.

[0040] The first main channel is communicated with the second sub-channel, and the second main channel is communicated with the fourth sub-channel.

[0041] According to an embodiment of the present application, before the first reagent and / or the second reagent is pushed out through the fourth pipeline, the first electromagnetic valve, the third pipeline, and the second electromagnetic valve, the method further comprises:

[0042] The second pipeline is filled with the second reagent.

[0043] The first pipeline, the third pipeline, and the fourth pipeline are filled with the first reagent.

[0044] According to an embodiment of the present application, the first reagent and / or the second reagent is pushed out through the fourth pipeline, the first electromagnetic valve, the third pipeline, and the second electromagnetic valve further comprises:

[0045] The first reagent and the second reagent are pushed out continuously; or

[0046] The first reagent is pushed out first, and then the second reagent is pushed out after a predetermined time interval; or

[0047] The second reagent is pushed out first, and then the first reagent is pushed out after a predetermined time interval.

[0048] The third pipeline has a volume greater than the amount of the first reagent added at one time.

[0049] According to an embodiment of the present application, the continuously pushing out the first reagent and the second reagent comprises:

[0050] The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent;

[0051] The first main channel is communicated with the second branch channel, and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline;

[0052] The first main channel is communicated with the second branch channel, and the second main channel is communicated with the fourth branch channel, and the first reagent in the third pipeline and the second reagent in the fifth pipeline are continuously pushed out of the fifth pipeline.

[0053] According to an embodiment of the present application, the first pushing out the first reagent and then pushing out the second reagent after a predetermined time interval comprises:

[0054] The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent;

[0055] The first main channel is communicated with the second branch channel, and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline;

[0056] The first main channel is communicated with the second branch channel, and the second main channel is communicated with the fourth branch channel, and the first reagent in the fifth pipeline is pushed out;

[0057] The second reagent is pushed out after a predetermined time interval;

[0058] The fifth pipeline has a volume equal to the amount of the first reagent added at one time.

[0059] According to an embodiment of the present application, the first pushing out the second reagent and then pushing out the first reagent after a predetermined time interval comprises:

[0060] The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent;

[0061] The first main channel is communicated with the second branch channel, and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline;

[0062] communicate the first main channel with the second branch channel and the second main channel with the fourth branch channel, push the second reagent in the third pipeline out;

[0063] After a predetermined time interval, the first reagent is pushed out.

[0064] To solve the above technical problems, another technical solution adopted by the present application is to provide an analyzer comprising the liquid adding device.

[0065] To solve the above technical problems, another technical solution adopted by the present application is to provide a liquid adding method based on the liquid adding device, comprising:

[0066] The first reagent is sucked through the fourth pipeline, the first electromagnetic valve and the first pipeline.

[0067] The second reagent is sucked through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve and the second pipeline.

[0068] The first reagent and / or the second reagent is pushed out through the fourth pipeline, the first electromagnetic valve, the third pipeline and the second electromagnetic valve.

[0069] When the second reagent is sucked, the second reagent is buffered in the third pipeline.

[0070] The present application has the following advantages: Different from the prior art, the liquid adding device, the analyzer and the liquid adding method provided by the present application share one suction device for the first reagent and the second reagent, and the second reagent is buffered in the third pipeline without entering the suction device, which avoids the cavitation phenomenon of the second reagent in the suction device and further avoids the generation of bubbles from the source, thereby improving the accuracy of reagent adding. BRIEF DESCRIPTION OF DRAWINGS

[0071] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative effort based on these drawings, wherein:

[0072] Figure 1 is a schematic diagram of the liquid adding device provided by the present application. DETAILED DESCRIPTION

[0073] With reference to the accompanying drawings, the technical solutions in the embodiments of the present application will be described clearly and completely. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative effort belong to the scope of protection of the present application.

[0074] It should be noted that if the embodiments of the present application involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative positional relationship, movement condition, etc. between components in a certain posture (as shown in the drawings), and if the certain posture changes, the directional indications also change accordingly.

[0075] In addition, if the embodiments of the present application involve descriptions such as "first", "second", etc., the descriptions of "first", "second", etc. are only for description purposes, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the fact that a person of ordinary skill in the art can realize it, and when the combination of technical solutions contradicts each other or cannot be realized, it should be considered that the combination of technical solutions does not exist, and is not within the scope of protection claimed by the present application.

[0076] Please refer to Figure 1 The embodiment of the present application provides a liquid adding device 10, which comprises a first electromagnetic valve 110, a second electromagnetic valve 120, a first pipeline 130, a second pipeline 140, a third pipeline 150, a fourth pipeline 160 and a suction device 170.

[0077] The first pipeline 130 is connected with the first electromagnetic valve 110; the second pipeline 140 is connected with the second electromagnetic valve 120; the third pipeline 150 connects the first electromagnetic valve 110 and the second electromagnetic valve 120; and the fourth pipeline 160 connects the first electromagnetic valve 110 and the suction device 170.

[0078] The suction device 170 is used for:

[0079] sucking the first reagent through the fourth pipeline 160, the first electromagnetic valve 110 and the first pipeline 130;

[0080] sucking the second reagent through the fourth pipeline 160, the first electromagnetic valve 110, the third pipeline 150, the second electromagnetic valve 120, the second pipeline 140;

[0081] The first reagent and / or the second reagent are pushed out through the fourth pipeline 160, the first electromagnetic valve 110, the third pipeline 150 and the second electromagnetic valve 120.

[0082] The second reagent is buffered in the third pipeline 150.

[0083] The first reagent is placed in the first container 210, and the second reagent is placed in the second container 220. The second reagent is preferably a liquid with a small consumption, high cost or easy to produce bubbles, such as a hemolytic agent or a specific protein agent.

[0084] In the embodiment of the present application, the first reagent and the second reagent share one suction device 170, and the second reagent is buffered in the third pipeline 150 without entering the suction device 170, thereby avoiding the cavitation phenomenon of the second reagent in the suction device 170 and further avoiding the generation of bubbles from the source.

[0085] The scheme does not need to add new components or discard reagents, and simplifies the liquid path system and reduces the cost.

[0086] Specifically, the first electromagnetic valve 110 has a first main channel 112, a first branch channel 114 and a second branch channel 116 in selective communication with the first main channel 112, and the first main channel 112 can be switched to be in communication with the first branch channel 114 or the second branch channel 116 by a switching button or a corresponding control program; the second electromagnetic valve 120 has a second main channel 122, a third branch channel 124 and a fourth branch channel 126 in selective communication with the second main channel 122, and the second main channel 122 can be switched to be in communication with the third branch channel 124 or the fourth branch channel 126 by a switching button or a corresponding control program.

[0087] In the embodiment of the present application, the first main channel 112 is connected with one end of the fourth pipeline 160; the first branch channel 114 is connected with one end of the first pipeline 130; the second branch channel 116 is connected with one end of the third pipeline 150; the second main channel 122 is connected with the other end of the third pipeline 150; the third branch channel 124 is connected with one end of the second pipeline 140; and the fourth branch channel 126 can be directly used as a liquid outlet, and the reagent can be pushed out through the fourth branch channel 126.

[0088] The liquid adding device 10 can further include a fifth pipeline 180 connected with the fourth branch channel 126, and the reagent can be pushed out through the fifth pipeline 180.

[0089] In the embodiment of the present application, the liquid adding device 10 further includes a reaction pool 190, which can be arranged at the liquid outlet end of the fourth branch channel 126 to directly receive the reagent pushed out through the fourth branch channel 126, or the reaction pool 190 is arranged at the liquid outlet end of the fifth pipeline 180 to receive the reagent pushed out through the fifth pipeline 180.

[0090] The suction device 170 can be a syringe or a dosing pump.

[0091] When the suction device 170 is a syringe, the liquid adding device 10 further comprises a stepper motor 172 for driving the syringe to move in a dosing manner to accurately control the dosing of the reagent.

[0092] The liquid adding device 10 provided by the embodiment of the present application has multiple states, wherein:

[0093] The foregoing suctioning of the first reagent through the fourth pipeline 160, the first electromagnetic valve 110, the first pipeline 130 comprises:

[0094] The foregoing suctioning of the second reagent through the fourth pipeline 160, the first electromagnetic valve 110, the third pipeline 150, the second electromagnetic valve 120, the second pipeline 140 comprises:

[0095] The foregoing pushing out of the first reagent and / or the second reagent through the fourth pipeline 160, the first electromagnetic valve 110, the third pipeline 150, the second electromagnetic valve 120 comprises:

[0096] The first main channel 112 and the second branch channel 116 are communicated, and the second main channel 122 and the fourth branch channel 126 are communicated.

[0097] In a specific application, the foregoing pushing out of the first reagent and / or the second reagent through the fourth pipeline 160, the first electromagnetic valve 110, the third pipeline 150, the second electromagnetic valve 120 comprises:

[0098] The second pipeline 140 is filled with the second reagent;

[0099] The first pipeline 130, the third pipeline 150, and the fourth pipeline 160 are filled with the first reagent;

[0100] The first main channel 112 and the second branch channel 116 are communicated, and the second main channel 122 and the third branch channel 124 are communicated, and then the stepper motor 172 drives the suction device 170 to make the second pipeline 140 filled with the second reagent.

[0101] The first main channel 112 and the first branch channel 114 are communicated, and the second main channel 122 and the fourth branch channel 126 are communicated, and then the stepper motor 172 drives the suction device 170 to make the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 filled with the first reagent.

[0102] In a specific application, the pushing out of the first reagent and / or the second reagent through the fourth pipeline 160, the first electromagnetic valve 110, the third pipeline 150, and the second electromagnetic valve 120 further comprises:

[0103] continuously pushing out the first reagent and the second reagent; or

[0104] first pushing out the first reagent and then pushing out the second reagent after a predetermined time interval; or

[0105] first pushing out the second reagent and then pushing out the first reagent after a predetermined time interval;

[0106] wherein the volume of the third pipeline 150 is greater than the amount of the second reagent added at one time, that is, the volume of the third pipeline 150 has sufficient buffering capacity. For example, the volume of the third pipeline 150 can be 500 microliters, and the amount of the second reagent added at one time can be 200 microliters. Of course, the specific volume and the actual amount of addition at one time do not constitute a limitation on the present application, and can be adjusted as needed.

[0107] The aforementioned continuously pushing out the first reagent and the second reagent comprises:

[0108] filling the first reagent in the first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180, wherein the smaller the volume of the fifth pipeline 180, the better;

[0109] communicating the first main channel 112 and the second branch channel 116 and communicating the second main channel 122 and the third branch channel 124, and sucking a certain amount of the second reagent into the third pipeline 150;

[0110] communicating the first main channel 112 and the second branch channel 116 and communicating the second main channel 122 and the fourth branch channel 126, and continuously pushing out the first reagent in the third pipeline 150 and the second reagent in the fifth pipeline 180 from the fifth pipeline 180.

[0111] The aforementioned first pushing out the first reagent and then pushing out the second reagent after a predetermined time interval comprises:

[0112] filling the first reagent in the first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180;

[0113] communicating the first main channel 112 and the second branch channel 116 and communicating the second main channel 122 and the third branch channel 124, and sucking a certain amount of the second reagent into the third pipeline 150;

[0114] communicating the first main channel 112 and the second branch channel 116 and communicating the second main channel 122 and the fourth branch channel 126, and pushing out the first reagent in the fifth pipeline 180 as a whole.

[0115] The second reagent is pushed out after a predetermined interval. During the interval, the first reagent can be allowed to react with other reagents for a period of time before the second reagent is added, and the specific type of the other reagents is not limited.

[0116] In this embodiment, the volume of the fifth pipeline 180 is equal to the amount of the first reagent added at one time. For example, if the amount of the first reagent added at one time is 100 microliters, the volume of the fifth pipeline 180 is designed to be 100 microliters.

[0117] The foregoing pushing out of the second reagent first and then pushing out of the first reagent after a predetermined interval includes:

[0118] The first pipeline 130, the third pipeline 150, and the fourth pipeline 160 are filled with the first reagent.

[0119] The first main channel 112 and the second branch channel 116 are communicated, and the second main channel 122 and the third branch channel 124 are communicated, and the second reagent is sucked into the third pipeline 150.

[0120] The first main channel 112 and the second branch channel 116 are communicated, and the second main channel 122 and the fourth branch channel 126 are communicated, and the second reagent in the third pipeline 150 is pushed out.

[0121] The first reagent is pushed out after a predetermined interval. During the interval, the second reagent can be allowed to react with other reagents for a period of time before the first reagent is added, and the specific type of the other reagents is not limited.

[0122] In this embodiment, the fifth pipeline 180 can be provided or not provided.

[0123] If the fifth pipeline 180 is not provided, the reagent can be pushed to the reaction pool 190 through the fourth branch channel 126 of the second electromagnetic valve 120.

[0124] If the fifth pipeline 180 is provided, the initial condition is that the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 are filled with the first reagent, and the fifth pipeline 180 is in an empty pipeline state without reagent. When the second reagent is sucked into the third pipeline 150, the second reagent is located in the front section of the third pipeline 150 (where "front" refers to the position close to the second electromagnetic valve 120), and the first reagent is located in the rear section of the third pipeline 150 (where "rear" refers to the position close to the first electromagnetic valve 110). At this time, the second reagent is pushed out by the stepping motor 172 driving the suction device 170 first, and the first reagent is pushed out after a predetermined interval. During the interval, the first reagent can be sucked back into the third pipeline 150 by the stepping motor 172 driving the suction device 170, so that the fifth pipeline 180 continues to be in an empty pipeline state without reagent.

[0125] In the above liquid adding modes, the first reagent and the second reagent share one suction device 170, and the second reagent is buffered in the third pipeline 150 without entering the suction device 170, thereby avoiding the cavitation phenomenon of the second reagent in the suction device 170 and further avoiding the generation of bubbles from the source. The scheme does not need to add new components or throw reagents, and simplifies the liquid path system and reduces the cost.

[0126] The application further provides an analyzer comprising the above liquid adding device and other analysis modules. The analyzer can be a blood cell analyzer or an immune analyzer.

[0127] Please continue to refer to Figure 1 The application further provides a liquid adding method based on the above liquid adding device 10, which comprises the following steps:

[0128] sucking the first reagent through the fourth pipeline 160, the first electromagnetic valve 110 and the first pipeline 130;

[0129] sucking the second reagent through the fourth pipeline 160, the first electromagnetic valve 110, the third pipeline 150, the second electromagnetic valve 120 and the second pipeline 140;

[0130] pushing out the first reagent and / or the second reagent through the fourth pipeline 160, the first electromagnetic valve 110, the third pipeline 150 and the second electromagnetic valve 120;

[0131] In the step of sucking the second reagent, the second reagent is buffered in the third pipeline 150.

[0132] In the application, the first reagent is placed in the first container 210, and the second reagent is placed in the second container 220. The second reagent is preferably a liquid with a small consumption, high cost or easy to produce bubbles, such as a hemolytic agent or a specific protein agent.

[0133] In the application, the first reagent and the second reagent share one suction device 170, and the second reagent is buffered in the third pipeline 150 without entering the suction device 170, thereby avoiding the cavitation phenomenon of the second reagent in the suction device 170 and further avoiding the generation of bubbles from the source.

[0134] The scheme does not need to add new components or throw reagents, and simplifies the liquid path system and reduces the cost.

[0135] The liquid adding device 10 provided in the application has multiple states, wherein:

[0136] The foregoing drawing of the first reagent through the fourth conduit 160, the first solenoid valve 110, the first conduit 130 includes:

[0137] The foregoing drawing of the second reagent through the fourth conduit 160, the first solenoid valve 110, the third conduit 150, the second solenoid valve 120 includes:

[0138] The foregoing pushing out of the first reagent and / or the second reagent through the fourth conduit 160, the first solenoid valve 110, the third conduit 150, the second solenoid valve 120 includes:

[0139] The first main channel 112 is communicated with the second branch channel 116 and the second main channel 122 is communicated with the fourth branch channel 126.

[0140] In a specific application, the foregoing pushing out of the first reagent and / or the second reagent through the fourth conduit 160, the first solenoid valve 110, the third conduit 150, the second solenoid valve 120 includes:

[0141] The second conduit 140 is filled with the second reagent;

[0142] The first conduit 130, the third conduit 150, the fourth conduit 160 are filled with the first reagent;

[0143] The first main channel 112 is communicated with the second branch channel 116 and the second main channel 122 is communicated with the third branch channel 124, and then the suction device 170 is driven by the stepping motor 172 to fill the second conduit 140 with the second reagent.

[0144] The first main channel 112 is communicated with the first branch channel 114 and the second main channel 122 is communicated with the fourth branch channel 126, and then the suction device 170 is driven by the stepping motor 172 to fill the first conduit 130, the third conduit 150, the fourth conduit 160 with the first reagent.

[0145] In a specific application, the foregoing pushing out of the first reagent and / or the second reagent through the fourth conduit 160, the first solenoid valve 110, the third conduit 150, the second solenoid valve 120 includes:

[0146] The first reagent and the second reagent are continuously pushed out; or

[0147] The first reagent is pushed out first, and then the second reagent is pushed out after a predetermined time interval; or

[0148] The second reagent is pushed out first, and then the first reagent is pushed out after a predetermined time interval.

[0149] The volume of the third pipeline 150 is greater than the amount of the second reagent added at one time, that is, the volume of the third pipeline 150 has sufficient buffering capacity. For example, the volume of the third pipeline 150 can be 500 microliters, and the amount of the second reagent added at one time can be 200 microliters. Of course, the specific volume and the actual amount of reagent added at one time do not constitute a limitation on the present application, and can be adjusted as needed.

[0150] The foregoing continuous pushing out of the first reagent and the second reagent includes:

[0151] The first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180 are filled with the first reagent, and the volume of the fifth pipeline 180 is as small as possible;

[0152] The first main channel 112 is communicated with the second branch channel 116, and the second main channel 122 is communicated with the third branch channel 124, so that a certain amount of the second reagent is sucked into the third pipeline 150;

[0153] The first main channel 112 is communicated with the second branch channel 116, and the second main channel 122 is communicated with the fourth branch channel 126, so that the first reagent in the third pipeline 150 and the second reagent in the fifth pipeline 180 are continuously pushed out of the fifth pipeline 180.

[0154] The foregoing pushing out of the first reagent first and then pushing out of the second reagent after a predetermined time interval includes:

[0155] The first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180 are filled with the first reagent;

[0156] The first main channel 112 is communicated with the second branch channel 116, and the second main channel 122 is communicated with the third branch channel 124, so that a certain amount of the second reagent is sucked into the third pipeline 150;

[0157] The first main channel 112 is communicated with the second branch channel 116, and the second main channel 122 is communicated with the fourth branch channel 126, so that the first reagent in the fifth pipeline 180 is pushed out as a whole;

[0158] The second reagent is pushed out after a predetermined time interval. During the interval, the first reagent can first react with other reagents, and the second reagent is added after a period of reaction. The specific types of other reagents are not limited.

[0159] In this embodiment, the volume of the fifth pipeline 180 is equal to the amount of the first reagent added at one time. For example, the amount of the first reagent added at one time is 100 microliters, and the volume of the fifth pipeline 180 is designed to be 100 microliters.

[0160] The foregoing pushing the second reagent first and pushing the first reagent after a predetermined interval includes:

[0161] The first conduit 130, the third conduit 150, and the fourth conduit 160 are filled with the first reagent;

[0162] The first main channel 112 and the second sub-channel 116 are communicated, and the second main channel 122 and the third sub-channel 124 are communicated, so that a quantitative second reagent is sucked into the third conduit 150;

[0163] The first main channel 112 and the second sub-channel 116 are communicated, and the second main channel 122 and the fourth sub-channel 126 are communicated, so that the second reagent in the third conduit 150 is pushed out;

[0164] The first reagent is pushed out after a predetermined interval, and in the interval, the second reagent can first react with other reagents, and after a period of reaction, the first reagent is added. The specific category of other reagents is not limited.

[0165] In the embodiment, the fifth conduit 180 can be provided or not provided.

[0166] If the fifth conduit 180 is not provided, the reagent can be pushed to the reaction pool 190 through the fourth sub-channel 126 of the second electromagnetic valve 120.

[0167] If the fifth conduit 180 is provided, the initial condition is that the first conduit 130, the third conduit 150, and the fourth conduit 160 are filled with the first reagent, the fifth conduit 180 is in an empty pipe state without reagent, when a quantitative second reagent is sucked into the third conduit 150, the second reagent is located in the front section of the third conduit 150 (wherein "front" refers to the position close to the second electromagnetic valve 120), and the first reagent is located in the rear section of the third conduit 150 (wherein "rear" refers to the position close to the first electromagnetic valve 110), at this time, the suction device 170 is driven by the stepping motor 172 to push out the second reagent first, and then the first reagent is pushed out after a predetermined interval, and in the interval, the first reagent can also be sucked back into the third conduit 150 by the suction device 170 driven by the stepping motor 172, so that the fifth conduit 180 continues to be in an empty pipe state without reagent.

[0168] In the above several liquid adding modes, the first reagent and the second reagent share one suction device 170, and the second reagent is buffered in the third conduit 150 without entering the suction device 170, which avoids the cavity phenomenon of the second reagent in the suction device 170, and further avoids the generation of bubbles from the source. This scheme does not need to increase new components or discard reagents, and simplifies the liquid path system and reduces the cost.

[0169] The above merely illustrates the embodiments of the present application, and does not limit the patent scope of the present application, and any equivalent structure or equivalent process transformation, or direct or indirect application in other related technical fields, which are made by using the content of the present application specification and drawings, are also included in the patent protection scope of the present application.

Claims

1. A liquid adding device characterized by comprising: The liquid adding device comprises a first electromagnetic valve, a second electromagnetic valve, a first pipeline, a second pipeline, a third pipeline, a fourth pipeline and a suction device; The first pipeline is connected with the first electromagnetic valve; The second pipeline is connected with the second electromagnetic valve; The third pipeline connects the first electromagnetic valve with the second electromagnetic valve; The fourth pipeline connects the first electromagnetic valve with the suction device; The suction device is used for: sucking the first reagent through the fourth pipeline, the first electromagnetic valve and the first pipeline; sucking the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve and the second pipeline; pushing out the first reagent and / or the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline and the second electromagnetic valve; wherein when the second reagent is sucked, the second reagent is buffered in the third pipeline; The first electromagnetic valve has a first main channel, a first branch channel and a second branch channel which are in selective communication with the first main channel; The second electromagnetic valve has a second main channel, a third branch channel and a fourth branch channel which are in selective communication with the second main channel; The first main channel is connected with one end of the fourth pipeline; The first branch channel is connected with one end of the first pipeline; The second branch channel is connected with one end of the third pipeline; The second main channel is connected with the other end of the third pipeline; The third branch channel is connected with one end of the second pipeline; The fourth branch channel serves as a liquid outlet; The liquid adding device further comprises a fifth pipeline connected with the fourth branch channel; Before the first reagent and / or the second reagent is pushed out through the fourth pipeline, the first electromagnetic valve, the third pipeline and the second electromagnetic valve, the second pipeline is filled with the second reagent; The first pipeline, the third pipeline and the fourth pipeline are filled with the first reagent; The first reagent and / or the second reagent is pushed out through the fourth pipeline, the first electromagnetic valve, the third pipeline and the second electromagnetic valve further comprises: the first reagent and the second reagent are continuously pushed out; or the first reagent is first pushed out and then the second reagent is pushed out after a predetermined time interval; or the second reagent is first pushed out and then the first reagent is pushed out after a predetermined time interval; The volume of the third pipeline is greater than the use amount of the second reagent for one-time liquid adding; The liquid adding device further comprises a reaction pool which is arranged at the liquid outlet end of the fourth branch channel or the liquid outlet end of the fifth pipeline. The suction device is a syringe or a quantitative pump.

2. The liquid adding device according to claim 1, characterized by The liquid adding device further comprises a stepping motor which is used for driving the quantitative movement of the syringe.

3. The liquid adding device according to claim 2, characterized by The first main channel and the first branch channel are communicated and the second main channel and the fourth branch channel are communicated.

4. The liquid adding device according to claim 1, wherein The second reagent is sucked through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve and the second pipeline. ​ 5. The liquid adding device according to claim 1, wherein ​ The first main channel is communicated with the second branch channel and the second main channel is communicated with the third branch channel.

6. The liquid adding device according to claim 1, wherein The pushing out of the first reagent and / or the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline and the second electromagnetic valve comprises: The first main channel is communicated with the second branch channel and the second main channel is communicated with the fourth branch channel.

7. The liquid adding device according to claim 1, wherein The continuous pushing out of the first reagent and the second reagent comprises: The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent. The first main channel is communicated with the second branch channel and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline. The first main channel is communicated with the second branch channel and the second main channel is communicated with the fourth branch channel, and the first reagent in the third pipeline and the second reagent in the fifth pipeline are continuously pushed out of the fifth pipeline.

8. The liquid adding device according to claim 1, wherein The pushing out of the first reagent and the second reagent comprises: The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent. The first main channel is communicated with the second branch channel and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline. The first main channel is communicated with the second branch channel and the second main channel is communicated with the fourth branch channel, and the first reagent in the third pipeline and the second reagent in the fifth pipeline are continuously pushed out of the fifth pipeline. The pushing out of the first reagent and the second reagent comprises: The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent.

9. The liquid adding device according to claim 1, wherein The first main channel is communicated with the second branch channel and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline. The first main channel is communicated with the second branch channel and the second main channel is communicated with the fourth branch channel, and the first reagent in the third pipeline and the second reagent in the fifth pipeline are continuously pushed out of the fifth pipeline. The pushing out of the first reagent and the second reagent comprises: The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent. The first main channel is communicated with the second branch channel and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline.

10. An analyzer characterized by, The first main channel is communicated with the second branch channel and the second main channel is communicated with the fourth branch channel, and the first reagent in the third pipeline and the second reagent in the fifth pipeline are continuously pushed out of the fifth pipeline.

11. A method of dispensing a liquid based on the liquid dispensing device of claim 1, characterized in that, The pushing out of the first reagent and the second reagent comprises: The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent. The first main channel is communicated with the second branch channel and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline. The first main channel is communicated with the second branch channel and the second main channel is communicated with the fourth branch channel, and the first reagent in the third pipeline and the second reagent in the fifth pipeline are continuously pushed out of the fifth pipeline. The pushing out of the first reagent and the second reagent comprises:

12. The method of claim 11, wherein, The first pipeline, the third pipeline, the fourth pipeline and the fifth pipeline are filled with the first reagent. The first main channel is communicated with the second branch channel and the second main channel is communicated with the third branch channel, and the second reagent is sucked into the third pipeline. The first main channel is communicated with the second branch channel and the second main channel is communicated with the fourth branch channel, and the first reagent in the third pipeline and the second reagent in the fifth pipeline are continuously pushed out of the fifth pipeline. The first main channel is communicated with the second branch channel and the second main channel is communicated with the fourth branch channel. The analyzer comprises the liquid adding device of any one of claims 1-9. The analyzer comprises the liquid adding device of any one of claims 1-9. The first reagent is sucked through the fourth pipeline, the first electromagnetic valve and the first pipeline. The second reagent is sucked through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve and the second pipeline. The first reagent and / or the second reagent are pushed out through the fourth pipeline, the first electromagnetic valve, the third pipeline and the second electromagnetic valve. When the second reagent is sucked, the second reagent is buffered in the third pipeline. The first reagent is sucked through the fourth pipeline, the first electromagnetic valve and the first pipeline. The first main channel is communicated with the first branch channel and the second main channel is communicated with the fourth branch channel.

13. The method of claim 11, wherein, Sucking the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve comprises: So that the first main channel and the second branch channel are communicated, and the second main channel and the third branch channel are communicated.

14. The method of claim 11, wherein, Pushing out the first reagent and / or the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve comprises: So that the first main channel and the second branch channel are communicated, and the second main channel and the fourth branch channel are communicated.

15. The method of claim 11, wherein, Before pushing out the first reagent and / or the second reagent through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve comprises: The second pipeline is filled with the second reagent; The first pipeline, the third pipeline, the fourth pipeline are filled with the first reagent.

16. The method of claim 15, wherein, Pushing out the first reagent and the second reagent continuously through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve further comprises: Pushing out the first reagent first and then pushing out the second reagent after a predetermined time interval; or Pushing out the second reagent first and then pushing out the first reagent after a predetermined time interval; Wherein, the volume of the third pipeline is greater than the amount of the second reagent added at one time. The first reagent and the second reagent are continuously pushed out through the fourth pipeline, the first electromagnetic valve, the third pipeline, the second electromagnetic valve, which comprises:

17. The method of claim 16, wherein, The first pipeline, the third pipeline, the fourth pipeline, the fifth pipeline are filled with the first reagent; So that the first main channel and the second branch channel are communicated, and the second main channel and the third branch channel are communicated, the second reagent is sucked into the third pipeline; So that the first main channel and the second branch channel are communicated, and the second main channel and the fourth branch channel are communicated, the first reagent in the third pipeline and the second reagent in the fifth pipeline are continuously pushed out of the fifth pipeline. The first reagent is pushed out first and then the second reagent is pushed out after a predetermined time interval, which comprises:

18. The method of claim 16, wherein, The first pipeline, the third pipeline, the fourth pipeline, the fifth pipeline are filled with the first reagent; So that the first main channel and the second branch channel are communicated, and the second main channel and the third branch channel are communicated, the second reagent is sucked into the third pipeline; So that the first main channel and the second branch channel are communicated, and the second main channel and the fourth branch channel are communicated, the first reagent in the fifth pipeline is pushed out; The second reagent is pushed out after a predetermined time interval; Wherein, the volume of the fifth pipeline is equal to the amount of the first reagent added at one time. The second reagent is pushed out first and then the first reagent is pushed out after a predetermined time interval, which comprises:

19. The method of claim 16, wherein, The first pipeline, the third pipeline, the fourth pipeline are filled with the first reagent; So that the first main channel and the second branch channel are communicated, and the second main channel and the third branch channel are communicated, the second reagent is sucked into the third pipeline; ​ causing the first main channel to communicate with the second sub-channel and the second main channel to communicate with the fourth sub-channel, pushing the second reagent out of the third conduit; after a predetermined time interval, pushing the first reagent out.

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