Sample testing device and sample testing method

Abstract

The application discloses a sample detection device and a sample detection method. The sample detection device comprises a first container, a second container, a sample bin, a sample processing bin and a reaction bin. The sample bin is used for containing samples. The sample processing bin is used for mixing sample processing liquid with the samples to obtain a first mixed liquid. The reaction bin is used for mixing the first mixed liquid with reaction liquid to obtain a second mixed liquid. The first container is connected with the sample processing bin through a second pipeline. The sample bin is connected with the sample processing bin through a first pipeline. The sample processing bin is connected with the reaction bin through a third pipeline. The second container is connected with the reaction bin through a fourth pipeline. The first container contains processing liquid, and the second container contains reaction liquid. The first container can be pierced by a first ejection part, so that the processing liquid flows into the sample processing bin through the second pipeline. The second container can be pierced by a second ejection part, so that the reaction liquid flows into the reaction bin through the fourth pipeline. The application has simple and exquisite structure, and solves the technical problems that the prior art cannot uniformly perform sample storage, sample processing and detection, and the detection efficiency is low.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biological detection, in particular to a sample detection device and a sample detection method. BACKGROUND

[0002] With the continuous development of intelligent and convenient biological detection devices, the detection means when people encounter diseases is becoming more and more abundant. For example, when a hospital needs to collect and detect human blood to see if there is a pneumonia virus, a portable sample collector, i.e. a disposable blood collection device and a blood storage device, is often used to collect the blood sample of the case, and the blood sample is combined with a sample analysis device to detect the presence of virus antibody antigens in the blood sample of the case.

[0003] At present, the detection device for detecting proteins, esters, polysaccharides and genes usually mixes the sample with a sample processing liquid, and separately processes the mixed liquid, which is complicated and inefficient. The sample is often contaminated during the continuous transportation process, resulting in inaccurate detection results.

[0004] At present, there is no effective solution to the above problems. SUMMARY

[0005] The embodiments of the present application provide a sample detection device and a sample detection method to at least solve the technical problem that the prior art cannot uniformly perform sample storage, sample processing and detection, and the detection efficiency is low.

[0006] According to one aspect of the embodiments of the present application, a sample detection device is provided, which comprises a first container, a second container, a sample bin, a sample processing bin and a reaction bin; the sample bin is used to hold a sample; the sample processing bin is used to mix a sample processing liquid with the sample to obtain a first mixed liquid; the reaction bin is used to mix the first mixed liquid with a reaction liquid to obtain a second mixed liquid; the first container is connected to the sample processing bin through a second pipeline; the sample bin is connected to the sample processing bin through a first pipeline; the sample processing bin is connected to the reaction bin through a third pipeline; the second container is connected to the reaction bin through a fourth pipeline; the first container has a processing liquid therein, the second container has a reaction liquid therein, the first container can be pierced by a first ejection portion so that the processing liquid flows into the sample processing bin through the second pipeline; and the second container can be pierced by a second ejection portion so that the reaction liquid flows into the reaction bin through the fourth pipeline.

[0007] According to another aspect of the embodiment of the present application, a sample detection method is also provided, which comprises: keeping the air hole of the reaction chamber in a closed state, opening the air hole of the sample processing chamber, after pressing the first pressing part, the first ejection member punctures the first container, so that the sample processing liquid enters the sample processing chamber through the second pipeline;

[0008] After the sample is added into the sample chamber, and the second air hole is in a closed state, and the third pipeline is in a non-through state, the sample stays in the sample chamber;

[0009] After the pressing pressure providing part is pressed, the sample chamber is inflated, the sample enters the sample processing chamber through the first pipeline, and fills the sample processing chamber from bottom to top, and the air holes of the first container and the second container are kept closed;

[0010] The first air hole of the sample processing chamber is closed, and the second air hole of the reaction chamber is opened, so that the liquid in the sample processing chamber enters the reaction chamber due to air pressure;

[0011] After the second container is punctured, the reaction liquid in the second container enters the reaction chamber along the fourth pipeline;

[0012] After the pressing pressure providing part is pressed for multiple times, in view of the fact that the first air hole of the sample processing chamber is closed, the gas enters the reaction chamber through the first pipeline and the third pipeline, so that the sample processing liquid and the reaction liquid are fully mixed and react;

[0013] According to the reaction result, detection is performed.

[0014] In the embodiment of the present application, the sample processing, sample mixing and sample detection are integrated, the pressure device and the control of the conveying pipeline are used to fully mix and process the sample, so that the sample is detected efficiently and accurately, and the technical problems of the prior art, i.e. the sample cannot be collected, processed and detected uniformly, and the detection efficiency is low, are solved. BRIEF DESCRIPTION OF DRAWINGS

[0015] The drawings described herein are used to provide further understanding of the present application, and form a part of the present application. The schematic embodiments of the present application and the descriptions thereof are used to explain the present application, and do not constitute an improper limitation on the present application. In the drawings:

[0016] Figure 1 is a schematic view of the internal structure of a sample detection device according to an embodiment of the present application;

[0017] Figure 2 is a schematic view of a sample detection device comprising a shell.

[0018] Figure 3 is a flow chart of a sample detection method according to an embodiment of the present application.

[0019] Figure 4 is a specific flow chart of operation steps according to an embodiment of the present application. DETAILED DESCRIPTION

[0020] In order to make the technical personnel in the art better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor should belong to the scope of protection of the present application.

[0021] It should be noted that the terms "first", "second", and the like in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and do not necessarily indicate a specific order or sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments of the present application described herein can be implemented in an order other than that illustrated or described herein. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device including a series of steps or units does not necessarily limit to those steps or units clearly listed, but can include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0022] According to an embodiment of the present application, a sample detection method embodiment is provided. It should be noted that the steps shown in the flowchart of the accompanying drawings can be executed in a computer system such as a set of computer executable instructions, and although the logical order is shown in the flowchart, in some cases, the steps shown or described herein can be executed in an order different from that shown herein.

[0023] In the prior art, the sample collection and storage tube is usually used as the main device for placing the sample during the process of collecting and processing the sample, and then the sample processing liquid or reaction liquid is added to the sample collection tube, and mixed by machine or manually to achieve the purpose of detecting the sample. However, the existing sample detection method has the problems of non-closed detection space, inaccurate detection results caused by sample contacting with external air, complicated and complex detection steps, inconvenient operation and the like, resulting in low efficiency of the whole sample detection process.

[0024] Embodiment one

[0025] Figure 1 is a schematic diagram of the internal structure of a sample detection device according to an embodiment of the present application, as Figure 1As shown, the device includes: a first container 41, a second container 42, a sample chamber 10, a sample processing chamber 20, and a reaction chamber 30;

[0026] The sample chamber 10 is used to hold samples; the sample processing chamber 20 is used to mix the sample processing solution with the sample to obtain a first mixture; the reaction chamber 30 is used to mix the first mixture with a reaction solution to obtain a second mixture; the first container 41 is connected to the sample processing chamber 20 through a second pipe 52; the sample chamber 10 is connected to the sample processing chamber 20 through a first pipe 51; the sample processing chamber 20 is connected to the reaction chamber 30 through a third pipe 53; the second container 42 is connected to the reaction chamber 30 through a fourth pipe 54; the first container 41 contains the processing solution, and the second container 42 contains the reaction solution; the first container 41 can be pierced by a first ejector 71 located on the shell or outside, allowing the processing solution to flow into the sample processing chamber 20 through the second pipe 52; the second container 42 can be pierced by a second ejector 72 located on the shell or outside, allowing the reaction solution to flow into the reaction chamber 30 through the fourth pipe 54. The first ejector and the second ejector 72 will... Figure 2 The details are described in the text.

[0027] Figure 2 This is a schematic diagram of a sample detection device including a housing. Figure 2 As shown, optionally, the sample detection device also includes a housing 90, the housing of which may be made of plastic. For clarity, Figure 2 The components on the housing are indicated by thicker lines. The housing 90 includes a sample inlet 91 corresponding to the opening of the sample chamber 10 for injecting sample liquid. The sample liquid can flow along... Figure 2 The first conduit 51 shown fills the sample processing chamber 20 from bottom to top. Furthermore, the housing 90 also has a first pressing part 92 and a second pressing part 93. The first pressing part 92 is linked to a first ejector part 71 to puncture the first container 41. The second pressing part 93 is linked to a second ejector part 72 to puncture the second container 42. The first pressing part 92 and the first ejector part 71 can be integrally formed or assembled, and the second pressing part 93 and the second ejector part 72 can be integrally formed or assembled.

[0028] The first pressing part 92 and the second pressing part 93 are used to elastically deform to drive at least one of the first ejection part 71 and the second ejection part 72 to pierce at least one of the first container 41 and the second container 42. The first pressing part is arranged corresponding to the first container 41 for a user or a machine to press to use the first ejection part 71 to pierce the first container 41; the second pressing part is arranged corresponding to the second container 42 for a user or a machine to press to use the second ejection part 72 to pierce the second container 42.

[0029] It is worth noting that, Figure 1 And Figure 2 The figure shows a schematic diagram in a use state, i.e., the appearance of the sample detection device in an upright state, and the "upper" and "lower" described in the specification are all taken as examples in the use state, i.e., the upright state of the sample detection device, and are not particularly limited.

[0030] Specifically, the first pressing part 91 and the second pressing part 92 can be made of an elastic material, such as a plastic elastic piece. In the case of non-integral molding, the first ejection part 71 and the second ejection part 72 used to pierce the first container 41 and the second container 42 can be rigid piercing short needles. When the elastic material of the pressing piece is deformed, the ejection part on the pressing piece immediately pierces the container, ensuring that the liquid in the container is also transported out at the same time as the pressing piece is deformed under the influence of external force, for subsequent detection processing.

[0031] Optionally, at least one of the first container 41 and the second container 42 is made of aluminum foil.

[0032] Specifically, the first container 41 and the second container 42 can respectively load sample processing liquid and reaction liquid. The embodiment of the present application uses aluminum foil as the material of the first container and the second container. The aluminum foil is soft and easy to break, and the ejection part can easily pierce the aluminum foil. When the aluminum foil is used as the container material, the container achieves the best piercing response effect. It is worth noting that, in order to prevent both layers of the aluminum foil from being pierced when the aluminum foil is used as the material, a hard protrusion can be arranged on one layer of the aluminum foil bag to prevent the ejection part from piercing both layers of the aluminum foil bag at the same time. In order to prevent the problem of uneven pressing of the aluminum foil bag, the pressing part can be arranged to adapt to the shape of the aluminum foil bag to avoid uneven stress.

[0033] Optionally, the reaction liquid includes a reaction buffer or a reaction reagent.

[0034] Specifically, the reaction liquid can be a reaction buffer or a reaction reagent. The reaction buffer needs to be combined with the freeze-dried pellet to dissolve the freeze-dried pellet and mix with the first mixed liquid to react. For example, when the tester pokes the second container 42 with hands or a machine, the reaction buffer in the second container 42 enters the reaction chamber 30 along the fourth pipeline 54 to mix with the freeze-dried pellet to dissolve the freeze-dried pellet. For another example, in another case, the second container 42 stores a reaction reagent, and the reaction chamber 30 does not need to have a freeze-dried pellet. The reaction reagent in the second container 42 is directly squeezed into the reaction chamber 30. Optionally, the sample chamber 10 is conical, and the top of the sample chamber 10 has a cover body for sealing the sample chamber 10, so that the sample liquid in the sample chamber 10 cannot flow along the first pipeline 51 in a sealed state.

[0035] Optionally, the sample processing chamber 20 has a first air hole, and the liquid in the sample processing chamber 20 cannot flow along the third pipeline 53 in a sealed state. The reaction chamber 30 has a second air hole, and the liquid in the reaction chamber cannot flow along the fourth pipeline in a sealed state.

[0036] Optionally, in a use state, the position where the second pipeline 52 communicates with the sample processing chamber 20 is higher than the position where the first pipeline 51 communicates with the sample processing chamber 20. That is, the position where the second pipeline 52 communicates with the sample processing chamber 20 is closer to the position of the air hole of the reaction chamber 20 than the position where the first pipeline 51 communicates with the sample processing chamber 20.

[0037] Optionally, the sample detection device further comprises an air hole sealing device for sealing at least one of the first air hole and the second air hole. The sealing device is, for example, two, which are used to seal the first air hole 61 and the second air hole 62, respectively.

[0038] Specifically, the sealing device can be a plastic cap in interference fit with the air hole. By pulling out and plugging in the plastic cap, the air hole can be opened or closed.

[0039] Optionally, as shown in Figure 1 The sample detection device further comprises a pressure providing part 80 connected to the sample chamber 10 through a pressure providing pipeline for providing gas pressure to mix the sample in the sample chamber 10 with the sample processing liquid in the sample processing chamber 20. Corresponding to the pressure providing part 80, as shown in Figure 2As shown, the housing has a pressure providing presser 94 for a user or a machine to apply pressure. The pressure providing part 80 and the pressure providing presser 94 are used to change the shape of the pressure providing part by pressing the presser, so that gas from the gas pipeline connected with the sample chamber 10 enters the sample chamber 10, and the liquid in the sample chamber 10 is pressed into the sample processing chamber 20 through the first pipeline 51, and is fully mixed with the sample processing liquid therein.

[0040] Optionally, the pressure providing part 80 and the pressure providing presser 94 are made of resilient material, so that the pressure providing part 80 and the pressure providing presser 94 can rebound after being pressed.

[0041] Optionally, the first pipeline 51 to the fourth pipeline 54 are formed on the housing, and the sample processing chamber 20, the reaction chamber 30, and the first container 41 and the second container 42 are arranged in the housing. The housing may, for example, include a first housing and a second housing. The first housing is indicated by 90 in the figure, and the second housing and the first housing are used to accommodate the sample processing chamber 20, the reaction chamber 30, and the first container 41 and the second container 42, the first pipeline 51 to the fourth pipeline 54, and the pressure providing pipeline, for example, a flow channel integrally formed on the second housing, and the pipelines are formed by the alignment and buckling of the first housing and the second housing. The housing 90, for example, the first housing or the second housing, also has a sealing member for sealing the pipelines. The outside of the pipelines is sealed by rubber or other sealing materials.

[0042] According to another aspect of the embodiment of the present application, a sample detection terminal is also provided for adapting and installing the sample detection device, and the sample detection terminal includes an accommodating part for accommodating the sample detection device, and a gas hole blocking device for blocking the first gas hole and the second gas hole. The accommodating part may, for example, be a groove capable of accommodating the sample detection device.

[0043] According to another aspect of the embodiment of the present application, the sample detection terminal further includes a detection result display device for generating and displaying a detection result according to the second mixed liquid, and further includes an electric presser for pressing the pressure providing part.

[0044] In the embodiment of the present application, the sample processing, sample mixing, and sample detection are integrated, and the pressure device and the conveying pipeline are controlled, so that the sample is fully mixed and processed, and the sample is efficiently and accurately detected, thereby solving the technical problems that the sample cannot be accommodated, processed, and detected in the prior art, and the detection efficiency is low.

[0045] Embodiment Two

[0046] According to another aspect of the embodiments of the present application, a sample detection method is also provided, which comprises: injecting a sample processing liquid into a sample processing chamber 20; mixing the sample processing liquid with a sample to obtain a first mixed liquid; injecting the first mixed liquid into a sample reaction chamber 30; mixing the first mixed liquid with a reaction liquid to obtain a second mixed liquid; and generating a detection result according to the second mixed liquid.

[0047] Figure 3 is a flow chart of a sample detection method according to an embodiment of the present application, as shown in Figure 3

[0048] In step S301, a sample processing liquid is injected into a sample processing chamber 20.

[0049] Specifically, the purpose of the embodiments of the present application is to realize the process of storing and analyzing samples after collecting samples from a case or a sample source. Therefore, in order to preliminarily process the collected sample liquid, the sample processing liquid needs to be mixed with the sample to achieve the purpose of subsequent sample processing.

[0050] As shown in Figure 1 The air hole of the reaction chamber 30 is kept closed, the air hole of the sample processing chamber 20 is opened, the first container is pressed, the first ejector pierces the first container, and the sample processing liquid in the first container enters the sample processing chamber 20 through the second pipeline 52. The sample processing liquid needs to be mixed with the collected sample and analyzed. The air hole is opened to inject the sample processing liquid into the processing chamber by using the atmospheric pressure in the pipeline.

[0051] Optionally, the injection of the sample processing liquid into the sample processing chamber 20 comprises: opening the first air hole 61 of the sample processing chamber 20; and injecting the sample processing liquid from the sample processing liquid container into the sample processing chamber 20.

[0052] It should be noted that the container of the sample processing liquid can be an aluminum foil bag. The aluminum foil facilitates the piercing of the ejection part (such as a broken needle) to form a vacuum to release the sample processing liquid loaded in the aluminum foil bag.

[0053] In step S302, the sample processing liquid is mixed with the sample to obtain a first mixed liquid.

[0054] As shown in Figure 3 The pressure providing part 80 in the figure is pressed to inflate the sample chamber 10. Then the sample enters the sample processing chamber 20 through the first pipeline 51 and fills the sample processing chamber 20 from bottom to top, but will not overflow through the opening of the second pipeline 52. At this moment, the air holes of the first container and the second container are closed, and the sample processing liquid will not flow downward along the third pipeline 53.

[0055] ​Optionally, before mixing the sample processing solution with the sample to obtain a first mixture, the method further includes adding the sample to the sample chamber 10.

[0056] Specifically, the sample chamber 10 can be a conical structure, which makes it convenient for testing personnel to put the collected sample liquid into the sample chamber 10.

[0057] Step S303: Inject the first mixture into the sample reaction chamber 30.

[0058] Specifically, such as Figure 1 As shown, the first vent of the sample processing chamber 20 is closed, and the second vent of the reaction chamber 30 is opened. Due to the air pressure, the liquid in the sample processing chamber 20 enters the reaction chamber 30. The liquid in the sample processing chamber 20 is the first mixture mentioned above. That is, the first mixture is injected into the reaction chamber 30 by controlling the air pressure of the vent, waiting for further reaction and detection.

[0059] Step S304: Mix the first mixture with the reaction liquid to obtain the second mixture.

[0060] Specifically, after the first mixture enters the reaction chamber 30, the reaction liquid also needs to be injected into the reaction chamber 30 to react with the first mixture and obtain the test results. The container for the reaction liquid can be a second container, such as an aluminum foil bag. The aluminum foil is punctured using the same needle-punching method as the sample processing liquid, allowing the reaction liquid to be pressurized and sent into the reaction chamber 30.

[0061] Specifically, such as Figure 1 As shown, by repeatedly pressing the pressure supply unit 80, and simultaneously sealing the first vent in the sample processing chamber 20, gas can only enter the reaction chamber 30 through the first pipe 51 and the third pipe 53, thus ensuring thorough mixing of the sample processing solution and the reaction reagents. During this process, both the first container 41 and the second container 42 remain stationary after being punctured and the liquid is expelled. The air pressure seals the drain pipes of the first container 41 and the second container 42, preventing liquid backflow.

[0062] Optionally, when the reaction solution is the reaction buffer, the method further includes: mixing and dissolving the reaction buffer with the lyophilized powder beads.

[0063] Specifically, the reaction liquid can be a reaction buffer or a reaction reagent, wherein the reaction buffer needs to be combined with the freeze-dried pellet and mixed with the first mixed liquid. For example, when the tester pokes the second container 42, the reaction buffer in the second container 42 enters the reaction chamber 30 along the fourth pipeline 54, mixes with the freeze-dried pellet, and dissolves the freeze-dried pellet. For another example, in some other cases, the second container 42 stores a reaction reagent, and in this case, the freeze-dried pellet in the reaction chamber 30 is not needed, and the reaction reagent in the second container 42 is directly squeezed into the reaction chamber 30.

[0064] Step S305, generating a detection result according to the second mixed liquid.

[0065] Specifically, the detection module can be arranged in the reaction chamber 30, and the detection result of the reaction chamber 30 is detected in terms of chroma or turbidity, so as to realize detection of the reaction result in the reaction chamber 30, and the detection result can be fed back to the intelligent terminal carrier. The intelligent terminal carrier can be a PC terminal or a mobile terminal with a detection device slot.

[0066] Figure 4 As shown in the specific flowchart of the operation steps of the present application. Figure 4 As shown in the specific flowchart of the operation steps of the present application.

[0067] Step S401, keeping the air hole of the reaction chamber 30 closed, opening the second air hole 61 of the sample processing chamber 20, after pressing the first pressing part of the shell, the first ejector 71 pierces the first container 41, and the sample processing liquid in the first container 41 enters the sample processing chamber 20 through the second pipeline 52;

[0068] Step S402, adding a sample in the sample chamber, as shown in the figure. Figure 2 At this time, the second air hole 62 is in a closed state, and the third pipeline 53 is not open, so that the sample stays in the sample chamber 10.

[0069] Step S403, pressing the pressure providing part 80 of the Figure 3 , and inflating the sample chamber 10, so that the sample enters the sample processing chamber 20 through the first pipeline 51 and fills the sample processing chamber 20 from bottom to top, but at this time, it will not overflow through the opening of the second pipeline. At this time, the air holes of the first container 41 and the second container 42 are kept closed, and the sample processing liquid will not flow down along the third pipeline. Press the pressure providing part 80 several times, and the sample processing liquid and the sample in the sample processing chamber 20 are fully mixed.

[0070] Step S404, closing the first air hole 61 of the sample processing chamber 20 and opening the second air hole 62 of the reaction chamber, and the liquid in the sample processing chamber 20 enters the reaction chamber 30 due to air pressure.

[0071] Step S405, the second container 42 is punctured, so that the reaction liquid in the second container 42 enters the reaction chamber 30 along the fourth pipeline and mixes with the freeze-dried powder pellets in the reaction chamber 30, so that the freeze-dried powder pellets are dissolved. In other cases, the reaction reagent is stored in the second container 42, and in this case, the freeze-dried powder pellets are not needed in the reaction chamber, and the reaction reagent in the second container 42 is directly pressed into the reaction chamber 30.

[0072] Step S406, after the pressure providing part is pressed multiple times, the first gas hole 61 in the sample processing chamber is closed, and the gas can only enter the reaction chamber through the first pipeline 51 and the third pipeline 53, so that the sample processing liquid and the reaction reagent are fully mixed. During this process, the first container 41 and the second container 42 remain stationary after the liquid is pressed out, and the gas pressure will close the liquid discharge pipeline of the first container 41 and the second container 42, so as to prevent the liquid from flowing back.

[0073] Step S407, the reaction result is detected.

[0074] In the embodiment of the present application, the sample processing, sample mixing and sample detection are integrated, and the pressure device and the conveying pipeline are controlled, so that the sample is fully mixed and processed, and the sample is accurately detected, thereby solving the technical problems that the sample cannot be collected, processed and detected in the prior art, and the detection efficiency is low.

[0075] In the several embodiments provided in the present application, it should be understood that the disclosed technology can be implemented in other ways. Of course, the unit embodiment described above is only illustrative, and for example, the division of the units can be a logical function division, and in actual implementation, another division mode can be used, for example, a plurality of units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the coupling or direct coupling or communication connection between the units shown or discussed can be indirect coupling or communication connection through some interfaces, units or modules, and can be electrical or other forms.

[0076] The units described as separate components can or can not be physically separated, and the components shown as units can or can not be physical units, i.e., they can be located in one place or distributed on multiple units. Part or all of the units can be selected according to actual needs to achieve the purpose of the embodiment.

[0077] In addition, each functional unit in each embodiment of the present application can be integrated in one processing unit, or each unit can exist physically, or two or more units can be integrated in one unit. The integrated unit can be realized in the form of hardware or in the form of a software functional unit.

[0078] The integrated unit, if implemented in the form of a software function unit and sold or used as an independent product, can be stored in a computer readable storage medium. Based on such understanding, the technical solutions of the present application, essentially or in other words, the part that contributes to the prior art or the whole or part of the technical solutions can be embodied in the form of a software product. The computer software product is stored in a storage medium, including a number of instructions to make a computer device (which can be a personal computer, a server or a network device, etc.) execute all or part of the steps of the methods described in various embodiments of the present application. The aforementioned storage medium includes: a U disk, a read-only memory (ROM, Read-Only Memory), a random access memory (RAM, Random Access Memory), a mobile hard disk, a magnetic disk or an optical disk, and various media that can store program codes.

[0079] The above is only the preferred embodiment of the present application, it should be pointed out that, for those skilled in the art, without departing from the principles of the present application, can make a number of improvements and refinements, these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A sample detection device, characterized by, The sample detection device comprises a first container, a second container, a sample container, a sample processing container and a reaction container. The sample container is used for containing a sample; the sample processing container is used for mixing a sample processing liquid with the sample to obtain a first mixed liquid; the reaction container is used for mixing the first mixed liquid with a reaction liquid to obtain a second mixed liquid; the first container is connected to the sample processing container through a second pipeline; The sample container is connected to the sample processing container through a first pipeline; the sample processing container is connected to the reaction container through a third pipeline; the second container is connected to the reaction container through a fourth pipeline; The first container contains a sample processing liquid, and the second container contains a reaction liquid; the first container can be pierced by a first ejection part, so that the sample processing liquid flows into the sample processing container through the second pipeline and reacts with the sample in the sample processing container; the second container can be pierced by a second ejection part, so that the reaction liquid flows into the reaction container through the fourth pipeline; The sample detection device further comprises a housing having a first pressing part and a second pressing part; the first pressing part can be elastically deformed to drive the first ejection part to pierce the first container, so that the liquid in the first container flows into the sample processing container; the second pressing part can be elastically deformed to drive the second ejection part to pierce the second container, so that the liquid in the second container flows into the reaction container; The sample detection device further comprises a pressure providing part connected to the sample container, which is used to provide air pressure to mix the sample in the sample container with the sample processing liquid in the sample processing container.

2. The apparatus of claim 1, wherein, At least one of the first container and the second container is made of aluminum foil.

3. The apparatus of claim 1, wherein, The top of the sample container has a cover body sealing the sample container, so that the sample in the sample container cannot flow along the first pipeline in the sealed state.

4. The apparatus of claim 1, wherein, In the use state, the position where the second pipeline communicates with the sample processing container is higher than the position where the first pipeline communicates with the sample processing container.

5. The apparatus of claim 1, wherein, The sample processing container has a first air hole through which liquid in the sample processing container cannot flow along the third pipeline in a blocked state; the reaction container has a second air hole through which liquid in the reaction container cannot flow along the fourth pipeline in a blocked state.

6. The apparatus of claim 5, wherein, The sample detection device further comprises an air hole blocking device for blocking at least one of the first air hole and the second air hole.

7. The apparatus of claim 1, wherein, The pressure providing part is made of a resilient material, so that the pressure providing part can rebound after being pressed.

8. The apparatus of claim 1, wherein, The first to fourth pipelines are formed on the housing, and the sample processing container, the reaction container, the first container and the second container are arranged in the housing.

9. The apparatus of claim 8, wherein, The housing further has a sealing member for sealing the pipelines.

10. A sample testing terminal for adapting a sample testing device according to any one of claims 1 to 9, characterized in that The sample detection terminal comprises a containing part for containing the sample detection device, and an air hole blocking device for blocking the first air hole and the second air hole.

11. The terminal according to claim 10, further comprising a detection result display device for generating and displaying a detection result based on the second mixed solution.

12. The terminal according to claim 11, further comprising an electrically operated member for pressing the pressure providing portion.

13. A method of detecting a sample, characterized by, including: keeping the air hole of the reaction chamber closed and opening the air hole of the sample processing chamber, after pressing the first pressing portion, the first ejector pierces the first container, so that the sample processing solution enters the sample processing chamber through the second pipeline; after adding the sample into the sample chamber, and the second air hole is closed, and the third pipeline is not connected, so that the sample stays in the sample chamber; after pressing the pressure providing portion, the sample chamber is inflated, the sample enters the sample processing chamber through the first pipeline, and fills the sample processing chamber from bottom to top, the air holes of the first container and the second container remain closed; closing the first air hole of the sample processing chamber and opening the second air hole of the reaction chamber, so that the liquid in the sample processing chamber enters the reaction chamber due to air pressure; after piercing the second container, the reaction liquid in the second container enters the reaction chamber along the fourth pipeline; after pressing the pressure providing portion for multiple times, under the premise that the first air hole of the sample processing chamber is closed, the gas enters the reaction chamber through the first pipeline and the third pipeline, so that the sample processing solution and the reaction liquid are fully mixed and react; detecting according to the reaction result.

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