A method for repairing the intestine by using probiotics to reduce heavy metal residues in the body

Abstract

The application provides a method for repairing intestine by using probiotics to reduce heavy metal residues in vivo, and is characterized by comprising the following steps: (1) separating microorganisms from yoghurt from a pasture in the Qinghai-Tibet Plateau; (2) preparing MRS medium and PBS solution; (3) inoculating and culturing; (4) strain detection; (5) analyzing the antioxidant capacity of probiotics; (6) analyzing the heavy metal resistance of probiotics; and (7) analyzing the heavy metal adsorption capacity of probiotics. The method applies probiotics with strong antioxidant capacity to the production of yoghurt, reduces the oxidation pressure in the intestine by eating probiotic yoghurt, protects the intestinal flora, and makes the microbial community better play a role in reducing heavy metal residues. The method has the advantages of broad-spectrum heavy metal removal, good removal effect, simple operation, low price, and the ability to colonize in the intestine and continuously play a role.

Description

Technical Field

[0001] This invention relates to the field of microbial genetic engineering, specifically to a probiotic strain and a method for intestinal repair that uses this strain to reduce heavy metal residues in the body. Background Technology

[0002] Heavy metal pollution has become a global problem. In recent years, due to the discharge of heavy metal industrial wastewater and the impact of daily human activities, approximately 46,700 hectares of heavy metal pollution occur annually in my country. 2 Land is contaminated with heavy metals. Heavy metals enter the human body through the food chain, posing a serious threat to human health. For example, cadmium poisoning can lead to osteoporosis, joint pain, and kidney failure, while chromium poisoning can damage the liver, kidneys, and endocrine glands, causing disease. Currently, heavy metal pollution in soil is mainly addressed through physical remediation, chemical remediation, and phytoremediation. However, these methods are time-consuming, expensive, and prone to secondary pollution. Furthermore, current heavy metal remediation primarily occurs in the environment, and considering the vast extent of heavy metal pollution globally, the area that these methods can remediate is extremely limited, leaving humans still inevitably threatened by heavy metals. Therefore, there is an urgent need to find a safe and effective method for heavy metal remediation.

[0003] The gut is the largest immune organ in the human body. Besides its digestive, absorptive, and secretory functions, it also plays a crucial role in improving the body's immune system. At least 1000 different types of microorganisms reside in the human gut, and these microorganisms play a vital role in regulating gut function. Studies have shown that germ-free mice, compared to normal mice, exhibit neurodevelopmental defects, lymphatic hypoplasia, and weakened immune function. Gut microbiota influence various physiological activities of the gut, including development, absorption, and immunity. Therefore, gut function can be improved by regulating the composition of the gut microbiota.

[0004] Probiotics are a type of live microorganism that colonizes the human gut and is beneficial to the body. Probiotics from the Qinghai-Tibet Plateau can not only directly repair heavy metal toxicity, but also indirectly repair it by regulating (or reshaping) the gut microbiota. The method of using probiotics for gut repair is safe, effective, simple to operate, inexpensive, and has a long-lasting effect. It can not only reduce the residue of heavy metals in the body, but also enhance the body's immunity by regulating the composition and metabolism of the gut microbiota.

[0005] Problems with existing technology:

[0006] (1) Currently, the main method for detoxifying heavy metals in the human body is to use chelating agents. However, this method has significant side effects, such as the risk of carcinogenicity and teratogenicity. In addition, due to the poor degradation of most chelating agents, they will cause secondary pollution to the environment after being excreted from the intestines.

[0007] (2) Currently, some extracts with antioxidant capabilities are also used to exert antioxidant effects. For example, extracts of fungi such as Ganoderma lucidum spore powder and truffles have strong antioxidant capabilities; plant extracts such as mulberry branches, apricot leaves and jujube leaves also have antioxidant capabilities. Taking these extracts can also reduce oxidative stress in the intestines. However, compared with probiotics, the extraction process of extracts is more complicated, the production cost is higher, and the time of action in the gastrointestinal tract is shorter, requiring long-term consumption.

[0008] (3) Existing methods for reducing heavy metal residues in the body using probiotics select strains based on their adsorption capacity for heavy metals, with each strain targeting a single heavy metal. However, the current environment is polluted by multiple heavy metals, so the scope of existing probiotics for removing heavy metals is relatively small. Summary of the Invention

[0009] In view of the shortcomings of existing technologies, this invention provides a method for intestinal repair by using probiotics to reduce heavy metal residues in the body. This involves screening a new strain of probiotics and utilizing this strain to reduce heavy metal residues in the body through intestinal repair. The technical solution adopted by this invention is as follows:

[0010] 1. A probiotic strain that can reduce heavy metal residues in the body, wherein the probiotic strain is Pediococcus acidilactici QZ-01, which was deposited at CCTCC on November 14, 2019, with accession number M2019930.

[0011] 2. A method for isolating probiotics that can reduce heavy metal residues in the body, comprising:

[0012] (1) Isolation of microorganisms from yogurt from pastures on the Qinghai-Tibet Plateau; (2) Preparation of MRS medium and PBS solution; (3) Inoculation and culture; (4) Detection of bacterial strains; (5) Analysis of antioxidant capacity of probiotics; (6) Analysis of heavy metal resistance of probiotics; (7) Analysis of heavy metal adsorption capacity of probiotics.

[0013] 3. Among the screened probiotics, the strain with strong anti-heavy metal and antioxidant capabilities, Pediococcus lactis QZ-01, was selected for use in the next step of functional yogurt production.

[0014] 4. A method for making yogurt that reduces heavy metal residues in the body, comprising the following steps:

[0015] (1) Activation of probiotic strains; (2) Yogurt fermentation; (3) Animal experiment analysis of yogurt.

[0016] 5. Application of probiotic QZ-01 in reducing heavy metals in the body, including but not limited to Cr, Hg, Pb, and Ni.

[0017] 6. Application of probiotic QZ-01 in scavenging hydroxyl radicals and DPPH radicals.

[0018] 7. Application of probiotic QZ-01 in the adsorption of Cr, Hg, Pb and Ni.

[0019] 8. Application of probiotic QZ-01 in the repair of Cr-induced liver oxidative stress and damage.

[0020] 9. Application of probiotic QZ-01 in reducing the abundance of harmful bacteria while increasing the abundance of beneficial bacteria.

[0021] 10. Application of probiotic QZ-01 in upregulating the expression of genes related to Cr reduction and antioxidant activity in the gut microbiota.

[0022] 11. Application of probiotic QZ-01 in Cr resistance and reducing ability of upregulated genes with unknown function.

[0023] 12. Application of probiotic QZ-01 in intestinal repair to reduce heavy metal residues in the body.

[0024] Beneficial effects:

[0025] This application provides a novel strain and applies it to the production of yogurt. By consuming probiotic yogurt, the oxidative stress in the intestine is reduced, thereby protecting the intestinal flora and allowing the microbial community to better play its role in reducing heavy metal residues. This method has the advantages of broad-spectrum removal of heavy metals, good removal effect, simple operation, low price, and the ability to colonize in the intestine and continue to play a role. Attached Figure Description

[0026] Figure 1 The results of the isolation of probiotics in this invention;

[0027] Figure 2 The results of yogurt preparation according to the present invention;

[0028] Figure 3 This invention demonstrates the hydroxyl radical and DPPH radical scavenging capabilities of different probiotics.

[0029] Among them, hydroxyl radicals (A) and DPPH radicals (B).

[0030] Figure 4 The results show the resistance of different probiotics to heavy metals Cr, Hg, Pb, and Ni.

[0031] Among them, Cr(A), Hg(B), Pb(C), and Ni(D).

[0032] Figure 5 This describes the grouping of animals in the animal experiments of this invention;

[0033] Figure 6 A represents the daily food intake of mice according to the present invention; B represents the effect of Cr(VI) and probiotics of the present invention on the growth rate of mice.

[0034] Figure 7 The effect of probiotics QZ-01 and XS40 on chromium content in mouse feces and tissues;

[0035] Among them, mouse feces (A) and tissue (B).

[0036] Figure 8 The present invention describes the repair effect of probiotics QZ-01 and XS40 on Cr(VI)-induced liver damage in mice.

[0037] Figure 9 A represents the quantitative study results of QZ-01 in the feces of the probiotic QZ-01 treatment group of this invention; B represents the content of QZ-01 in the feces after 20 days of four different treatments; C and D represent the reducing capacity of Cr(VI) of culturable microorganisms in feces after anaerobic culture at 37℃ for 72 hours and 44 hours, respectively.

[0038] Figure 10 This invention relates to the composition of total bacteria and metabolically active bacteria in feces.

[0039] Among them, (A) the ratio of Firmicutes to Bacteroidetes; (B) the relative abundance of bacterial 16S rRNA genes at the family level; (C) the genus-level heatmap based on 16S rRNA sequencing; and (D) the classification of mRNA at the genus level for each sample.

[0040] Figure 11 Analysis of the resistance and reducing capacity of unknown functional genes differentially expressed in gut microbiota enhanced by the probiotic QZ-01 of this invention to Cr(VI);

[0041] Among them, (A) the distribution of Cr repair-related genes in DEGS; (B) the growth of engineered E. coli strains in LB medium containing 1 Mm Cr(VI); (C) the Cr(VI) reduction rate of engineered E. coli BL21 containing the GCrR gene; (D) transmission electron microscopy and energy dispersive spectroscopy analysis of engineered E. coli treated with 1 mM Cr(VI) and untreated E. coli; (E) the evolutionary relationship of GCrR with known chromate reductases of different genera; (F) homologous sequence analysis of GCrR; and (G) structural modeling of 53431.

[0042] Figure 12 This is a schematic diagram of the mechanism of "intestinal repair" in this invention;

[0043] Figure 13 This invention relates to the liver and kidney function test report of student A.

[0044] Figure 14 This is the blood routine test report of student A in this invention;

[0045] Figure 15 This is the liver and kidney function test report for student B in this invention;

[0046] Figure 16 This is the blood routine test report of student B in this invention;

[0047] Figure 17 This is the liver and kidney function test report for student C in this invention.

[0048] Figure 18 This is the blood routine test report of student C in this invention;

[0049] Figure 19 This invention relates to the liver and kidney function test report of student Ding.

[0050] Figure 20 This invention relates to the blood routine test report of student Ding.

[0051] Figure 21 This is the liver and kidney function test report for student Wu in this invention;

[0052] Figure 22 This is the blood routine test report for student Wu in this invention. Detailed Implementation

[0053] To make the objectives, technical solutions, and advantages of the invention clearer, specific embodiments of the invention will be described in detail below with reference to the accompanying drawings. Examples of these preferred embodiments are illustrated in the drawings. The embodiments of the invention shown in and described with reference to the drawings are merely exemplary, and the invention is not limited to these embodiments.

[0054] It should also be noted that, in order to avoid obscuring the technical solution of the present invention due to unnecessary details, only the structures and / or processing steps closely related to the solution according to the present invention are shown in the accompanying drawings, while other details that are not closely related are omitted.

[0055] Example 1

[0056] This embodiment provides a method for isolating probiotics that can reduce heavy metal residues in the body, including:

[0057] 1. Isolation of microorganisms from yogurt from pastures on the Qinghai-Tibet Plateau

[0058] Make homemade yogurt from herders on the Qinghai-Tibet Plateau. First, collect fresh yak milk and pour it into a pot to heat over low heat. The temperature should not be too high, just hot enough to touch. Set aside after heating. Take an appropriate amount of yogurt prepared the day before and add it to the heated fresh milk, stirring well. Then, place it in an environment of about 42℃ to ferment for 3-4 hours.

[0059] 2. Prepare MRS culture medium and PBS solution

[0060] Place 63.3g of MRS agar into an Erlenmeyer flask, add 1000mL of distilled water, seal with a cotton plug, and sterilize at 121℃ for 20min. Weigh 0.8g of NaCl, 0.02g of KCl, 0.364g of Na2HPO4·12H2O, and 0.024g of KH2PO4 into an Erlenmeyer flask, add 100mL of distilled water, stir well, adjust the pH to 7.4, and sterilize.

[0061] 3. Inoculation and Culture

[0062] Pour approximately 30 mL of sterile MRS solid medium into petri dishes in a laminar flow hood. Seal each dish with sealing film and invert for later use. Add 5 mL of PBS solution to a test tube using a pipette. Dilute the PBS solution with a small amount of yogurt. Spread the diluted yogurt onto the MRS solid medium and incubate at 37°C for 18 hours.

[0063] 4. Microbial strain detection

[0064] As attached Figure 1 As shown in Table 1, single colonies were obtained, and then selected for colony PCR. The primers used were the universal 27F / 1492R primers. The dNTP Mixture, Taq enzyme, Taq Buffer, and other reagents required for PCR were purchased from Takara Bio Inc. The amplification program was as follows: 94℃ for 5 min, (95℃ for 30 s, 58℃ for 30 s, 72℃ for 90 s) for 35 cycles, and then 72℃ for 10 min. The amplified samples were then subjected to 16S rRNA sequencing. The selected strains are shown in Table 2, including *Pediococcus acidilactici*, *Lactobacillus fermentum*, *Lactobacillus plantarum*, *Lactobacillus acidophilus*, *Lactobacillus casei*, and *Lactobacillus bulgaricus*.

[0065] 5. Analysis of the antioxidant capacity of probiotics

[0066] The hydroxyl radical scavenging ability of several probiotics was determined. (A mixed solution was prepared by adding 1 mL of 0.75 mM phenanthroline, 1.5 mL of 0.15 M sodium phosphate buffer, 1 mL of 0.75 mM FeSO4, and 1 mL of 0.01% H2O2. Then, 1 mL of the sample to be tested was added. After mixing, the mixture was incubated at 37°C for 30 min, and the absorbance at 536 nm was measured. In the assay system, the absorbance of deionized water was used as the control, not the absorbance of H2O2. Hydroxyl radical scavenging ability = [A] 样品 -A 空白 ] / [A 对照 -A 空白 DPPH free radical scavenging ability (1 mL of sample and 1 mL of 0.2 mM DPPH solution (dissolved in methanol) were mixed and reacted in the dark for 30 minutes. Phosphate buffer was used as a blank, and the absorbance of the mixture at 517 nm was measured. DPPH free radical scavenging rate = [A] 空白 -A 样品 ] / A 空白 (See attached) Figure 3 As shown, QZ-01 has the highest hydroxyl radical scavenging ability and DPPH radical scavenging ability, with a hydroxyl radical scavenging rate of over 80% and a DPPH radical scavenging rate of over 50%.

[0067] 6. Heavy metal resistance analysis of probiotics

[0068] Probiotics screened using MRS activation; sterilized Ni 2+ Cd 2+ Cr 6+ Pb 2+ Add the strains separately to the culture media; inoculate each culture medium with 1% of the activated strain; incubate overnight at 37°C in a shaker at 150 rpm; then measure the OD600 value using a visible spectrophotometer. Each experiment was performed in triplicate. (See attached image.) Figure 4 As shown, QZ-01 exhibits the strongest resistance to several heavy metal ions.

[0069] 7. Analysis of the heavy metal adsorption capacity of probiotics

[0070] Probiotics were cultured in MRS medium at 37°C for 18 hours. After centrifugation, the dry and wet weights of the bacteria were measured. The probiotic cells were then resuspended in PBS buffer to a concentration of 5 g / L (wet weight). Ni was then added to the bacterial suspension. 2+ Cd 2+ Cr 6+ Pb 2+The heavy metal ions were equalized to a final concentration of 20 mM. The pH was adjusted to 6 with NaOH and HCl. The sample was incubated at 28°C for 2 h, centrifuged, the supernatant was discarded, and the precipitate was collected. The sample was digested with a concentrated nitric acid-perchloric acid (4:1, v / v) mixture at 300°C for 10 min, and the content of each heavy metal was determined by flame atomic absorption spectrometry.

[0071] The results showed that the strain of Pediococcus lactis QZ-01, which had strong anti-heavy metal and antioxidant capabilities, was selected from the screened probiotics and used for the next step of functional yogurt production.

[0072] Example 2

[0073] This embodiment provides a method for making yogurt by reducing heavy metal residues in the body, including the following steps:

[0074] 1. Activation of probiotic strains

[0075] The probiotic strain preserved on MRS slant was inoculated into skim milk (purchased from BD Company, USA) (milk powder: water = 1:8, w / v) and cultured at 37℃-42℃ for the first activation. The culture time was determined according to the specific curd condition. The culture was completed when the yogurt was uniformly coagulated and a small amount of whey was separated on the surface of the curd.

[0076] 2. Yogurt Fermentation

[0077] Add 3%-10% sucrose to the prepared skim milk, preheat at 50℃ for 10 minutes, then sterilize at 95℃ for 5 minutes. Let the skim milk cool to 42℃. Inoculate the skim milk with 2%-5% activated *Pediococcus lactis*, *Lactobacillus plantarum*, *Streptococcus thermophilus*, and *Lactobacillus bulgaricus*, and ferment at 42℃ for 4-6 hours. After fermentation, refrigerate at 4℃ for 12 hours. The prepared yogurt is shown in the attached image. Figure 2 As shown.

[0078] 3. Animal Experiment Analysis of Yogurt

[0079] Animal experimental groupings are attached. Figure 5 The prepared yogurt was administered to mice via gavage using a gavage needle. 1g of yogurt was administered daily (1g of yogurt was dissolved in 500ul of sterile water) for 30 days.

[0080] (1) Analysis of daily food intake and growth rate of mice

[0081] Mouse weight and food intake were recorded weekly. Results are attached. Figure 6 Probiotics had no significant effect on the daily food intake of mice, but they increased the growth rate of the mice.

[0082] (2) The ability of probiotics to remove heavy metals

[0083] Mice were dissected and various organs (including liver, kidneys, and small intestine) were collected. The organs were digested with concentrated nitric acid, and the heavy metal content in the organs was determined by flame atomic absorption spectrometry. (See attached image) Figure 7 As shown, the heavy metal content in the mouse liver decreased by about 46%, in the kidney by about 43%, in the small intestine by about 88%, and in the feces by about 27%, indicating that probiotics effectively reduced the heavy metal residue in the mouse body.

[0084] (3) The repair effect of probiotics on Cr(VI)-induced oxidative stress and damage to the liver in mice

[0085] Analysis of the repair capabilities of probiotics on the liver and small intestine, as shown in the attached figure. Figure 8 Pediococcus lactis reversed Cr(VI)-induced changes in oxidative stress markers. Cr(VI) exposure causes significant liver damage, including cytoplasmic vacuolization, nuclear pyknosis, and chromatin condensation, which Pediococcus lactis alleviated. This indicates that QZ-01 has a significant inhibitory effect on Cr(VI)-induced inflammatory responses.

[0086] (4) Analysis of the reducing power of probiotics for Cr(VI)

[0087] As attached Figure 9 As shown in Figure A, QZ-01 seedlings were planted starting on day 8, reaching their maximum planting density on day 20, after which the density stabilized. (See attached figure.) Figure 9 As shown in Figure B, quantitative analysis of QZ-01 in feces from different treatment groups revealed that QZ-01 can effectively colonize the intestine. Figure 9 As shown in C and 9D, the reducing capacity of fecal microbiota in fecal samples from different treatment groups for Cr(VI) was analyzed. The results showed that QZ-01 significantly improved the reducing capacity of fecal microbiota for Cr(VI).

[0088] (5) Analysis of gut microbiota composition using 16S rRNA sequencing and metagenomic sequencing

[0089] like Figure 10As shown in Figure A, at the phylum level, the proportions of Firmicutes and Bacteroidetes differed significantly among different sequencing methods. 16S rRNA sequencing analysis showed no significant difference in this proportion among different treatment groups, while metagenomic sequencing analysis showed that the proportion was significantly higher in the QZ-01 repair group than in other groups. This result suggests that active metabolic bacteria may play an important role in intestinal repair. At the family level, 16S rRNA analysis showed high abundances of S24-7 (12.47-42.03%), Prevotellaceae (10.88-36.07%), Bacteroidaceae (4.57-19.66%), Lachnospiraceae (2.92-15.20%), and Paraprevotellaceae (0.46-15.37%). Figure 10 B). The heatmap at the genus level showed that the community structure of the QZ-01 repair group was closer to that of the control group than that of the Cr(VI) group, indicating that QZ-01 had a mitigating effect on Cr(VI) toxicity. Figure 10 C). Significant difference analysis showed that, compared with the Cr(VI) group, QZ-01 significantly reduced the abundance of harmful bacteria, such as Mucispirillum (p = 2.60e-4) and Prevotella (p = 5.94e-3), while significantly increasing the abundance of beneficial bacteria, such as Blautia (p = 0.020). Compared with the control group, the QZ-01-only treatment group showed a significant increase in Parabacteroides (p = 0.016), Pediococcus (p = 0.038), and Parapravotella (p = 0.046), which, along with q-RT PCR results, indicates that QZ-01 effectively colonizes the gut. Figure 10As shown in Figure D, metagenomic sequencing further identified the active metabolizing bacteria in feces. Cr(VI) significantly altered the structure of the gut microbiota, significantly increasing the relative abundance of Bacteroides, Clostridium, Parabacteroides, and Helicobacter, while significantly decreasing the relative abundance of no-rank Lachnospiraceae and Eubacterium. Consumption of QZ-01 fully or partially restored the relative abundance of Eubacterium (from 1.48% to 8.55%), no-rank Lachnospiraceae (from 4.95% to 5.84%), Helicobacter (from 1.71% to 0.18%), Parabacteroides (from 7.15% to 4.89%), and Clostridium (from 7.67% to 4.48%). These results demonstrate the restorative effect of the probiotic QZ-01 on Cr(VI)-induced changes in the gut microbiota.

[0090] (6) Probiotic QZ-01 upregulated the expression of genes related to Cr(VI) reduction and antioxidant activity in the gut microbiota.

[0091] As shown in Table 2, compared with the Cr(VI) group, the probiotic QZ-01 upregulated the expression of thioredoxin, desulfurized iron redox protein, flavin reductase, and FAD / NAD(P)-binding oxidoreductase, among other proteins related to Cr(VI) reduction. It also upregulated the expression of major erythrin protein family proteins, thiol peroxidase, erythroredoxin, peroxidase, superoxide dismutase, and catalase, which are associated with oxidative stress.

[0092] (7) Analysis of Cr(VI) resistance and reducing ability of QZ-01 upregulated genes with unknown function

[0093] As attached Figure 11 As shown in A, 788 genes upregulated in QZ-01Cr(VI) were screened from 57,248 single genes, including 20 known Cr(VI) reduction-related genes, 14 known antioxidant-related genes, 546 other annotated genes, and 208 genes with unknown functions. Figure 11 B involved randomly selecting 10 genes with high abundance from 208 genes with unknown functions for gene synthesis and constructing corresponding E. coli engineered strains (unknown 1-10). The results showed that 5 of these engineered strains (unknown 2, 6, 7, 8, 9) significantly improved resistance to Cr(VI). Further investigation revealed that the engineered strain E. coli unknown9 / GCrR possessed a strong reducing ability against Cr(VI). Figure 11C). Transmission electron microscopy (TEM) scans of E. coli GCrR revealed intracellular Cr(III) precipitation, indicating that this strain can reduce Cr(VI) intracellularly. Figure 11 D). Figure 11 E represents a phylogenetic analysis of the GCrR gene, indicating that GCrR belongs to a branch of the known chromium reductase ChrR. Figure 11 F represents the comparison results of the amino acid sequence of GCrR with amino acids of known structures such as 4ICI_A, 4J8P_A, 3KLB_A, and CAH09789.1, with similarities of 33.12%, 29.09%, 32.87%, and 33.56%, respectively. Figure 11 G is a structural simulation diagram of GCrR, which shows that GCrR binds to a molecule of FMN. GCrR transfers electrons from the donor to Cr(VI) through FMN, thereby reducing Cr(VI) to Cr(III).

[0094] (8) Mechanism of "intestinal repair" using probiotics

[0095] As attached Figure 12 As shown, Cr(VI) entering the animal body increases the oxidative stress in the intestines and liver, increases the residue of Cr(VI) in the small intestine, liver and kidneys, causing liver damage and leading to intestinal flora imbalance. Probiotics can effectively colonize the intestines and reduce intestinal oxidative stress, protect the intestinal flora so that it can function better, reduce Cr(VI) and reduce heavy metal residues in the body, thereby protecting liver function.

[0096] (9) Safety verification through human trials

[0097] Ten student volunteers consumed 250g of probiotic yogurt prepared according to the method of this invention daily for one month. Blood samples were collected to measure liver function, kidney function, complete blood count, and other physiological indicators. The normal ranges for aspartate aminotransferase and alanine aminotransferase were 1-49 U / L, the normal range for total protein was 60-82 U / L, and the normal range for total bilirubin was 9.1-30.1 U / L. (See attached image) Figure 13 , 15 As shown in Figures 17, 19, and 21, the students' test results were all within the normal range; therefore, it can be concluded that consuming probiotic yogurt did not damage liver function. The normal range for urea is 2.9-7.1 U / L, and the normal range for creatinine is 44-108 U / L; all student reports showed normal results. The normal range for red blood cells is 4-10 × 10⁹ / L, and the normal range for white blood cells is 3.5-5.0 × 10¹² / L; the normal range for hemoglobin is 110-150 g / L. (See attached figure.) Figure 14 , 16As shown in figures 18, 20, and 22, the student test results all fluctuated within the normal range. The student experiments demonstrate that consuming probiotic yogurt will not cause harm to the human body, indicating that this probiotic yogurt is safe and reliable.

[0098] The above description is only a specific embodiment of this application. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of this application, and these improvements and modifications should also be considered within the scope of protection of this application.

[0099] Table 1. Strain Isolation Information

[0100]

[0101]

[0102] Table 2. Upregulated expression of genes related to Cr(VI) reduction, antioxidant genes, and some genes with unknown functions by probiotic QZ-01.

[0103]

[0104]

Claims

1. A probiotic strain that can reduce heavy metal residues in the body, wherein the probiotic strain is Pediococcus acidilactici QZ-01, which was deposited at CCTCC on November 14, 2019, with accession number M2019930.

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