A portable handheld solid phase extraction device and its matching assembly cover and portable reagent storage and liquid adding box

Abstract

The present invention discloses a portable handheld solid phase extraction device, including a solid phase extraction column, a centrifuge tube, and an assembly cover. The solid phase extraction column is placed in the centrifuge tube, and the upper end of the tube mouth and the inner wall of the assembly cover are rotatably connected; the centrifuge tube tube mouth is threadedly connected to the lower end of the assembly cover; the assembly cover is cylindrical with an opening at the bottom, and is composed of a top surface and a side wall. The top surface of the assembly cover is provided with a through hole, and the inner surface of the lower end of the side wall is provided with an internal thread. The inner surface of the side wall is provided with an inwardly protruding card slot step above the internal thread and below the lower surface of the top surface, and two card slot notches are provided in the middle of the card slot step. The device can also include a portable reagent storage and liquid adding box for storing and adding reagents when going out for on-site testing. The present invention uses the assembly cover to assemble the solid phase extraction column and the centrifuge tube into a whole, which can be disassembled and assembled, and can be used as needed without the need for additional bracket devices and test tube racks, which greatly reduces the volume of the device and effectively saves space and operation time.

Description

Technical Field

[0001] The invention relates to the technical field of chemical experimental instruments, and in particular to a portable handheld solid phase extraction device and a matching assembly cover and a portable reagent storage and liquid adding box. Background Art

[0002] With the development of detection technology and the improvement of on-site inspection requirements, rapid detection technology has received widespread attention. The main interference factor affecting the detection speed is the pretreatment time, so how to improve the efficiency of pretreatment has received widespread attention. The most important point in the pretreatment operation is sample purification, especially for samples containing various pigments and other impurities, which must be purified before being tested on the machine, otherwise these interfering substances will affect the accuracy of the test results. Generally, solid phase extraction columns can be used to purify samples, such as C18 solid phase extraction columns. However, the existing solid phase extraction columns need to be activated with an activation reagent before use, and there are steps such as loading, extraction, and elution during use. The solid phase extraction column needs to be installed on a specific bracket and equipped with a sample receiving device below. This results in the solid phase extraction column purification process requiring additional bracket equipment to operate. At the same time, the entire operation is time-consuming and costly. It can generally only be used in the laboratory and is not convenient for on-site testing. Summary of the invention

[0003] The present invention is aimed at the deficiencies in the prior art and provides a portable handheld solid phase extraction device and its matching assembly cover and portable reagent storage and liquid adding box. The device assembles the solid phase extraction column and the centrifuge tube for receiving the liquid through the assembly cover, and can be operated directly by hand without the need for an additional fixed bracket and a test tube rack. A portable reagent storage and liquid adding box matching the device is provided to store the activation reagent. According to the detection needs, a quantitative activation reagent is added to the reagent kit in advance, and the reagent can be added directly after simple assembly at the detection site, which reduces the cumbersomeness of carrying reagent bottles and quantitative sampling devices when adding the activation reagent on site.

[0004] To solve the above problems, the technical solution adopted by the present invention is as follows

[0005] A portable handheld solid phase extraction device comprises a solid phase extraction column, a centrifuge tube and an assembly cover, wherein the solid phase extraction column is placed in the centrifuge tube, the upper end of the solid phase extraction column and the inner wall of the assembly cover are rotatably connected; the centrifuge tube mouth is threadedly connected to the lower end of the assembly cover; the assembly cover is cylindrical with an opening at the bottom, and consists of a top surface and a side wall, the top surface of the assembly cover is provided with a through hole, the inner surface of the lower end of the side wall is provided with an internal thread matched with the external thread of the centrifuge tube mouth, the inner surface of the side wall is provided with an inwardly protruding slot step above the internal thread and below the lower surface of the top surface, and two slot notches are provided in the middle of the slot step.

[0006] When assembling and using the portable handheld solid phase extraction device, the two ends of the elliptical protruding step surface of the tube mouth of the solid phase extraction column are inserted from the notch of the card slot, and screwed into the gap above the card slot step, fixed on the card slot step, so that the solid phase extraction column and the assembly cover are rotated and locked, and then the centrifuge tube is put on the solid phase extraction column, and the tube mouth of the centrifuge tube is threadedly connected with the lower end of the assembly cover, and the portable handheld solid phase extraction device is obtained.

[0007] Furthermore, the through hole on the top surface of the assembly cover is arranged at the center of the top surface, the outlet of the through hole is located above the tube opening of the solid phase extraction column, and the diameter of the through hole is less than or equal to the tube opening diameter of the solid phase extraction column.

[0008] Furthermore, the through hole on the top surface of the assembly cover is arranged in a funnel shape, the diameter of the bottom outlet of the funnel is less than or equal to the diameter of the tube mouth of the solid phase extraction column, and the diameter of the upper inlet of the funnel is greater than the diameter of the tube mouth of the solid phase extraction column. When the diameter of the bottom outlet of the funnel is less than the diameter of the tube mouth of the solid phase extraction column, the bottom outlet of the funnel can be extended downward so that the bottom of the funnel is inserted into the tube mouth of the solid phase extraction column.

[0009] The slot step is arranged on the inner surface of the side wall, above the threaded portion and below the lower surface of the top surface. The slot step protrudes inwardly. The gap between the step and the lower surface of the top surface is the slot. The height of the slot is greater than or equal to the thickness of the protruding elliptical step of the solid phase extraction column tube mouth.

[0010] Two opposite notches are arranged in the middle of the slot step along the diameter direction of the assembly cover, namely the slot notches. The width of the slot notches along the circumferential direction is greater than the width of the elliptical protruding step surface of the solid phase extraction column tube orifice.

[0011] The diameter of the cavity between the two opposite ends of the slot step along the diameter direction of the assembly cover is smaller than the length of the two ends of the protruding elliptical step of the solid phase extraction column tube orifice.

[0012] The width of the slot step along the circumferential direction is greater than the width of the elliptical protruding step surface of the solid phase extraction column tube opening.

[0013] A vertical limiting column can be arranged at one end of the slot step, so that when the elliptical protruding step surface of the solid phase extraction column tube mouth is rotated and engaged, it stops at the limiting column.

[0014] The diameter and length of the centrifuge tube are respectively larger than the diameter and length of the solid phase extraction column, which is for assembly requirements.

[0015] Furthermore, it is preferred that at least one vent hole is provided at the slot notch of the side wall of the assembly cover, or at the side wall portion below the slot step and above the internal thread, in order to facilitate exhaust when the centrifuge tube receives liquid.

[0016] The portable handheld solid phase extraction device provided by the present invention is used by assembling the solid phase extraction column and the centrifuge tube into one body, holding the centrifuge tube with one hand, adding the activation reagent or the sample to be tested from the through hole of the assembly cover with the other hand, and then holding the centrifuge tube to wait for the column to be completed. No additional support device is required, which is convenient for on-site rapid detection.

[0017] In addition, the centrifuge tube used in the present invention is generally a conical bottom centrifuge tube commonly used on the market, but a conical flat bottom centrifuge tube can also be used. The conical flat bottom centrifuge tube is a conical bottom of an ordinary conical bottom centrifuge tube with a cylindrical tube wall extension arranged on the outer ring, and the length of the extension is greater than the height of the conical bottom, so that the centrifuge tube can stand upright by itself. In the case of a conical flat bottom centrifuge tube, the portable handheld solid phase extraction device provided by the present invention does not even need to be held by hand. The centrifuge tube of the device can be directly placed flat on a flat surface such as a desktop or the ground, and the sample can be added, activated, etc. There is no need to hold the hand and wait when passing the column, which further simplifies the column operation. Both conical bottom centrifuge tubes and conical flat bottom centrifuge tubes can be purchased on the market.

[0018] The present invention also provides an assembly cover for the above-mentioned portable handheld solid phase extraction device, wherein the assembly cover is cylindrical with an opening at the bottom, and consists of a top surface and a side wall. The top surface of the assembly cover is provided with a through hole, and the inner surface of the lower end of the side wall is provided with an internal thread. The inner surface of the side wall is provided with an inwardly protruding slot step and two slot notches arranged in the middle of the slot step above the internal thread and below the lower surface of the top surface.

[0019] The assembly cover is used to assemble the solid extraction column and the centrifuge tube together, and the solid extraction column is placed inside the centrifuge tube. The upper end of the solid extraction column is rotatably engaged with the slot step on the inner surface of the side wall of the assembly cover, and the centrifuge tube mouth is threadedly connected with the lower end of the assembly cover, forming a solid phase extraction device in which the solid extraction column is located inside the centrifuge tube and connected into one through the assembly cover, and can be handheld for operation.

[0020] Furthermore, the portable handheld solid phase extraction device may also include a portable reagent storage and liquid adding box, which is used to store the reagents carried and add reagents when going out for field testing. The portable reagent storage and liquid adding box is detachably connected to the assembly cover, and the portable reagent storage and liquid adding box is composed of a cylindrical base and a liquid storage cavity arranged above the base. The liquid storage cavity is provided with more than one, and the liquid storage cavity is a cavity with an upper opening, and a liquid outlet is provided at the bottom of the cavity. The liquid outlet is connected to the liquid outlet channel that vertically passes through the base, and the bottom outlet of the liquid outlet channel is provided above the through hole of the assembly cover; the base is provided with a sealing slot perpendicular to the direction of the liquid outlet channel, and the sealing slot penetrates the outer wall of the side of the base at one end toward the outside, and extends into the inner wall of the liquid outlet channel at one end toward the inside, and a sealing insert that matches the shape of the sealing slot is provided, and the length of the sealing insert is greater than the sealing slot; the upper end opening of the liquid storage cavity is provided with a sealing cover that can seal the liquid storage cavity.

[0021] The sealing insert is inserted into the sealing slot to seal the liquid outlet channel. When the sealing insert is taken out, the liquid outlet channel is opened.

[0022] Furthermore, the detachable connection between the portable reagent storage and liquid-adding box and the assembly cover can be a threaded connection, a snap connection or a rotational snap connection. The side wall of the base of the portable reagent storage and liquid-adding box is extended downward, and the extended side wall serves as a connecting portion, which is detachably connected to the upper end of the assembly cover. The connecting portion can be provided with an internal thread, a bayonet or a slot, and the upper end of the side wall of the assembly cover can be provided with an external thread that matches the internal thread, or a convex block that matches the snap or the slot, so that the assembly cover and the portable reagent storage and liquid-adding box can be detachably connected by thread or snap connection or rotational snap connection.

[0023] Furthermore, outwardly protruding lugs are provided at both ends of the side wall of the assembly cover in the diameter direction, and two slots with one end open and adapted to the lugs are provided on the extended side wall of the portable reagent storage and liquid-adding box. The lug is inserted into one end of the slot opening and rotated to the end of the slot, so that the assembly cover and the portable reagent storage and liquid-adding box are rotatably engaged.

[0024] Furthermore, at least one groove is provided on the top surface of the assembly cover from the outer edge of the through hole to the outer edge of the top surface in the radial direction, so that the assembly cover can be detachably connected with the portable reagent storage and liquid-adding box to provide internal air circulation. When the lug of the assembly cover is provided at the top end of the side wall of the assembly cover, the groove can also be provided at the position from the edge of the through hole to the outer end of the lug.

[0025] The upper opening of the liquid storage cavity of the portable reagent storage and liquid adding box is provided with a sealing cover for sealing the liquid.

[0026] The shape of the sealing cover is adapted to the opening shape of the liquid storage chamber. The sealing cover can also be fixedly connected to the outer wall of the liquid storage chamber through a soft plastic sheet.

[0027] The liquid storage cavity can store activation reagents, elution reagents, etc. When the sealing insert is inserted into the sealing slot, the reagent is added, and then the sealing cover is closed, which is convenient for carrying. When in use, the portable reagent storage and liquid adding box is connected to the upper end of the assembly cover, and the solid phase extraction column, the centrifuge tube, and the assembly cover are assembled, and then the sealing cover is opened, the sealing insert is pulled out, and the reagent can enter the solid phase extraction column from the liquid outlet of the liquid storage cavity, through the liquid outlet channel, and through the through hole of the assembly cover for activation or elution.

[0028] The bottom liquid outlet and the liquid outlet channel of the liquid storage cavity are preferably arranged near the center of the base, which is conducive to the lower end outlet of the liquid outlet channel being located above the through hole of the assembly cover.

[0029] When there are more than two liquid storage chambers, it is preferred that the center of the base is used as the center point and a plurality of liquid storage chambers are divided into a plurality of liquid storage chambers in a fan-shaped shape by a partition wall.

[0030] The bottom of the liquid storage chamber is preferably in the shape of a sloped funnel inclined toward the liquid outlet.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] 1. The portable handheld solid phase extraction device provided by the present invention can assemble a solid phase extraction column and a centrifuge tube into a whole, which can be disassembled and assembled and used as needed without the need for additional support devices and test tube racks, greatly reducing the size of the device and the cumbersome operation process, effectively saving space and saving operation time.

[0033] 2. The invention allows one hand to hold the centrifuge tube and the other hand to add reagents or samples, which is very suitable for on-site sampling and detection, simplifies column activation, greatly speeds up detection and improves detection quality. When using a pointed flat-bottom centrifuge tube, the device can be placed on a flat surface to stand upright without even holding it, which is more convenient for adding reagents or passing samples through the column.

[0034] 3. The centrifuge tube for receiving liquid can be disassembled and replaced according to the needs of receiving different reagents or samples, and the operation is easy.

[0035] 4. The device of the present invention can also be used in conjunction with a portable reagent storage and liquid-adding box, which can seal and store reagents. During storage and transportation, the reagents can be effectively prevented from mixing and leaking, making it easy to carry. When adding reagents, the assembly cover can be directly installed to add liquid without the need for an additional syringe or funnel, thereby simplifying the operation and adapting to different site requirements inside and outside the laboratory. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a cross-sectional plan view of a portable handheld solid phase extraction device.

[0037] Figure 2 This is a three-dimensional external view of the assembly cover.

[0038] Figure 3 A perspective view of the assembled cover.

[0039] Figure 4 A bottom perspective view of the assembled cover.

[0040] Figure 5 A sectional perspective view of the assembly cover.

[0041] Figure 6 It is a cross-sectional perspective view of a portable handheld solid phase extraction device.

[0042] Figure 7 Schematic diagram of the rotational connection between the solid phase extraction cartridge and the assembly cap.

[0043] Figure 8 An exploded perspective view of a portable reagent storage and liquid filling box.

[0044] Fig. 9 This is a bottom-up stereoscopic view of a portable reagent storage and liquid filling box.

[0045] Fig.10 A cross-sectional perspective view of a portable handheld solid phase extraction device equipped with a portable reagent storage and liquid addition box.

[0046] Fig.11 Another cutaway perspective view of the portable handheld solid phase extraction device after installing the portable reagent storage and liquid addition box.

[0047] Fig.12 A three-dimensional image of a portable reagent storage and liquid addition box containing three liquid storage chambers.

[0048] Fig.13 The bottom perspective view of a portable reagent storage and liquid addition box containing three liquid storage chambers.

[0049] Fig.14 It is a cross-sectional plan view of a portable handheld solid phase extraction device using a pointed flat-bottom centrifuge tube.

[0050] Fig.15 A cross-sectional view of a portable handheld solid phase extraction device using a pointed flat-bottom centrifuge tube and a portable reagent storage and liquid addition box. DETAILED DESCRIPTION

[0051] The technical solution of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0052] The solid phase extraction column and centrifuge tube used in the present invention are products that can be directly purchased on the market.

[0053] Example 1

[0054] The present invention provides a portable handheld solid phase extraction device, such as Figure 1 , Figure 6 As shown, it includes a solid phase extraction column 1, a centrifuge tube 2, and an assembly cover 3. The solid phase extraction column 1 is located inside the centrifuge tube 2, and the solid phase extraction column 1 and the inner wall of the assembly cover 3 are rotatably connected; the centrifuge tube 2 is placed outside the solid phase extraction column 1 and is threadedly connected to the lower end of the assembly cover 3; the assembly cover 3 is as shown in FIG. Figure 2 , 3As shown in , 4, and 5, it is a cylindrical shape with an opening at the bottom, consisting of a top surface 4 and a side wall 5. The top surface 4 of the assembly cover 3 is provided with a through hole 6, and the inner surface of the lower end of the side wall 5 is provided with an internal thread that matches the external thread of the tube mouth of the centrifuge tube 2. The inner surface of the side wall 5 is provided with an inwardly protruding card groove step 7 and two card groove notches 8 set in the middle of the card groove step 7 above the internal thread and below the lower surface of the top surface. When assembling and using the portable handheld solid phase extraction device, the two ends of the elliptical protruding step surface 9 of the tube mouth of the solid phase extraction column are inserted from the card groove notch 8, and screwed into the gap above the card groove step 7, and fixed on the card groove step 7, so that the solid phase extraction column 1 and the assembly cover 3 are rotatably engaged (as shown in FIG. Figure 7 As shown), the solid phase extraction column 1 is then covered with a centrifuge tube 2, and the mouth of the centrifuge tube is threadedly connected to the lower end of the assembly cover to obtain a portable handheld solid phase extraction device.

[0055] When the portable handheld solid phase extraction device provided by the present invention is used, the solid phase extraction column 1 and the centrifuge tube 2 are assembled into one body by using the assembly cover 3. The centrifuge tube 2 can be held in one hand, and the other hand can perform operations such as adding reagents, etc., without the need for an additional bracket device, which is convenient for on-site rapid detection.

[0056] The present invention also provides a mounting cover 3 for the above-mentioned portable handheld solid phase extraction device, such as Figures 2 to 5 As shown, the assembly cover is a cylindrical shape with an opening at the bottom, and is composed of a top surface 4 and a side wall 5. The top surface 4 of the assembly cover is provided with a through hole 6, and the inner surface of the lower end of the side wall 5 is provided with an internal thread. The inner surface of the side wall 5 is provided with an inwardly protruding slot step 7 and a slot notch 8 arranged in the middle of the slot step 7 above the internal thread and below the lower surface of the top surface.

[0057] Furthermore, the through hole 6 on the top surface of the assembly cover 3 is arranged at the center of the top surface 4 and is located above the tube opening of the solid phase extraction column 1. The diameter of the through hole 6 is less than or equal to the diameter of the tube opening of the solid phase extraction column 1.

[0058] Furthermore, the through hole 6 on the top surface of the assembly cover is arranged in a funnel shape, the diameter of the bottom outlet of the funnel is less than or equal to the diameter of the tube mouth of the solid phase extraction column, and the diameter of the upper inlet of the funnel is greater than the diameter of the tube mouth of the solid phase extraction column. When the diameter of the bottom outlet of the funnel is less than the diameter of the tube mouth of the solid phase extraction column, the bottom outlet of the funnel can be extended downward so that the bottom of the funnel is inserted into the tube mouth of the solid phase extraction column.

[0059] The slot step 7 is arranged on the inner surface of the side wall 5, above the threaded portion and below the lower surface of the top surface. The slot step 7 protrudes inward, and the gap between the step and the lower surface of the top surface is the slot. The height of the slot is greater than or equal to the thickness of the protruding elliptical step 9 of the solid phase extraction column tube mouth.

[0060] Two opposite notches are arranged in the middle of the slot step 7 along the diameter direction of the assembly cover, namely the slot notches 8. The width of the slot notches 8 along the circumferential direction is greater than the width of the elliptical protruding step surface 9 of the solid phase extraction column tube mouth.

[0061] The diameter of the cavity between the two opposite ends of the slot step 7 along the diameter direction of the assembly cover is smaller than the length of the two ends of the protruding elliptical step 9 of the solid phase extraction column tube orifice.

[0062] The width of the slot step 7 along the circumferential direction is greater than the width of the elliptical protruding step surface 9 of the solid phase extraction column tube opening.

[0063] A vertical limiting column 10 can be arranged at one end of the slot step 7 so that when the elliptical protruding step surface 9 of the solid phase extraction column tube mouth is rotated and engaged, it stops at the limiting column 10 .

[0064] The diameter and length of the centrifuge tube 2 are respectively larger than the diameter and length of the solid phase extraction column 1 for assembly purposes.

[0065] Further, preferably, at least one vent hole 11 is provided at the slot notch 8 of the side wall of the assembly cover, or at the side wall portion below the slot step 7 and above the internal thread, in order to facilitate exhaust when the centrifuge tube receives the column liquid in the solid phase extraction column. In this embodiment, two vent holes 11 are provided.

[0066] Example 2

[0067] The portable handheld solid phase extraction device provided by the present invention may also include a portable reagent storage and liquid addition box 12, which is used to store and add reagents when conducting field tests. The structure of the portable reagent storage and liquid addition box 12 is as follows: Figure 8-9 As shown, the portable reagent storage and liquid adding box 12 is detachably connected to the assembly cover 3, and the assembled portable handheld solid phase extraction device is as shown in FIG. Fig.10 , 11 shown.

[0068] The portable reagent storage and liquid-adding box 12 is composed of a cylindrical base 13 and a liquid storage cavity 14 arranged above the base. The liquid storage cavity 14 is provided with more than one, and two liquid storage cavities 14 are provided in this embodiment. The liquid storage cavity 14 is a cavity with an upper opening, and a liquid outlet 15 is provided at the bottom of the cavity. The liquid outlet 15 is connected to a liquid outlet channel 16 vertically passing through the base, and the bottom outlet of the liquid outlet channel is arranged above the through hole 6 of the assembly cover 3; the base 12 is provided with a sealing plug perpendicular to the direction of the liquid outlet channel 16. The sealing slot 17 has one end that penetrates the outer wall of the side of the base 12 and one end that extends into the inner wall of the liquid outlet channel 16, and a sealing insert 18 that matches the shape of the sealing slot 17 is provided. The length of the sealing insert 18 is greater than the sealing slot 17. The sealing insert 18 is inserted into the sealing slot 17 to seal the liquid outlet channel 16. When the sealing insert 18 is taken out, the liquid outlet channel 16 is opened. The upper end opening of the liquid storage chamber 14 is provided with a sealing cover 19 that can seal the liquid storage chamber 14.

[0069] Further, the detachable connection between the portable reagent storage and liquid-adding box 12 and the assembly cover 3 can be a threaded connection, a snap connection or a rotational snap connection. The side wall of the base 13 of the portable reagent storage and liquid-adding box is extended downward, and the extended side wall 20 is used as a connecting portion, which is detachably connected to the upper end of the assembly cover 3. The connecting portion 20 can be provided with an internal thread, a bayonet or a slot, and the upper end of the side wall 5 of the assembly cover 3 can be provided with an external thread that matches the internal thread, or a convex block that matches the snap or the slot, so that the assembly cover and the portable reagent storage and liquid-adding box are detachably threaded or snap-connected or rotationally snap-connected.

[0070] Furthermore, lugs 21 protruding outward are provided at both ends of the side wall 5 of the assembly cover in the diameter direction, such as Figure 2 As shown, two card slots 22 (with one end open and matched with the lugs 21) are provided on the extended side wall 20 of the portable reagent storage and liquid adding box. Figure 8 ,9), the lug 21 is inserted into one end of the opening of the card slot 22, and rotated to the end of the card slot 22, so that the assembly cover 3 is rotatably engaged with the portable reagent storage and liquid adding box 12. This is only one of the embodiments given, and the detachable connection between the assembly cover 3 and the portable reagent storage and liquid adding box 12 can also be achieved by other assembly methods.

[0071] Furthermore, on the top plane of the assembly cover 3, from the outer edge of the through hole 6 to the outer edge of the top surface 4, at least one downwardly recessed groove 23 is provided along the radial direction, so that after the assembly cover is detachably connected to the portable reagent storage and liquid adding box, internal air circulation is provided.

[0072] When using the optional card connection mode, such as Figure 8 , 9. When the lug 21 is provided on the side wall of the assembly cover, when the lug 21 is provided on the top of the side wall of the assembly cover, the groove 23 can also be provided at the position from the edge of the through hole to the lug 21 ( Figure 2shown).

[0073] The upper opening of the liquid storage cavity of the portable reagent storage and liquid adding box is provided with a sealing cover 19 for sealing the liquid.

[0074] The shape of the sealing cover 19 is adapted to the opening shape of the liquid storage chamber 14 .

[0075] The liquid storage chamber 14 can store activation reagents, elution reagents, etc. When the sealing insert 18 is inserted into the sealing slot 17, the reagents are added, and then the sealing cover 19 is covered, which is convenient for carrying. When in use, the portable reagent storage and liquid adding box 12 is connected to the upper end of the assembly cover 3 of the assembled portable handheld solid phase extraction device, and then the sealing cover 19 is opened, and the sealing insert 18 is pulled out, and the reagent can enter the solid phase extraction column 1 from the liquid outlet 15 of the liquid storage chamber, through the liquid outlet channel 16, and through the through hole 6 of the assembly cover to be activated or eluted.

[0076] The provision of more than one liquid storage cavity 14 does not mean that the entire base 13 is provided with liquid storage cavities 14. The base 13 may also be provided with a liquid outlet 15 and a liquid outlet channel 16 leading to the through hole 6 of the assembly cover at the top other than the liquid storage cavity 14, which can be directly used to add the sample to be tested. Of course, the sample can also be added by removing the portable reagent storage and liquid adding box and directly adding the sample to be tested into the through hole 6 of the assembly cover.

[0077] The bottom liquid outlet 15 and the liquid outlet channel 16 of the liquid storage chamber are preferably arranged near the center of the base, which is conducive to the lower end outlet of the liquid outlet channel 16 being located above the through hole 6 of the assembly cover.

[0078] Furthermore, the liquid outlet channel 16 is divided into two channels, an upper channel and an lower channel, by the sealing slot 17. Preferably, the aperture of the lower channel 16-2 is larger than that of the upper channel 16-1 to facilitate liquid outflow.

[0079] When there are two or more liquid storage chambers 14, it is preferred that the center of the base 13 is used as the center point and a plurality of liquid storage chambers are divided into a plurality of liquid storage chambers in a fan-shaped shape by partition walls.

[0080] The bottom of the liquid storage chamber 14 is preferably in the shape of a sloped funnel inclined toward the liquid outlet 15. Fig.10 shown.

[0081] After the sealing insert 18 is inserted into the sealing slot 17 for sealing, in order to prevent the outer end of the insert from falling outward, a fixing insert device, such as a lock or a buckle device, can be installed on the outer wall of the base to lock the outer end of the insert, and then open the lock or buckle when in use. Alternatively, a wide elastic rubber band can be used to clamp the outer wall to cover the outer end of the insert, and the wide rubber band can be removed when reagents need to be added.

[0082] Example 3

[0083] This embodiment is a portable reagent storage and liquid adding box with three liquid storage chambers, as shown in the three-dimensional diagram Fig.12 and Fig.13 As shown, the portable reagent storage and liquid-adding box is provided with three liquid storage chambers 14, and the sealing cover 19 at the opening of the liquid storage chamber 14 is fixedly connected to the outer wall of the liquid storage chamber 14 through a soft plastic sheet 24, so that the cover is not easy to be lost and is more convenient to open and close.

[0084] Example 4

[0085] In this embodiment, the centrifuge tube 2 in the portable handheld solid phase extraction device of Examples 1 and 2 is replaced with a pointed flat bottom centrifuge tube, such as Fig.14 , 15 As shown. The pointed flat bottom centrifuge tube is an ordinary pointed bottom centrifuge tube with a cylindrical tube wall extension 25 arranged on the outer circle of the conical pointed bottom. The length of the extension 25 is greater than the height of the conical pointed bottom, so that the centrifuge tube 2 can stand upright by itself. In the case of a pointed flat bottom centrifuge tube, the portable handheld solid phase extraction device provided by the present invention can be placed flat on a table or the ground without holding the centrifuge tube, and sample addition, activation, etc. can be performed. In particular, there is no need to hold the hand and wait when passing the column, which simplifies the operation.

[0086] Example 5

[0087] The portable handheld solid phase extraction device of Example 2 was used for column activation and sample extraction experiments. The solid phase extraction column was a C18 column with a specification of 200 mg / 3 mL, and the outer centrifuge tube was a centrifuge tube with a specification of 50 mL. The solid phase extraction column, the centrifuge tube and the assembly cover were assembled into one according to Example 2. The sealing inserts were respectively inserted into the sealing slots of the two liquid storage chambers, and 2.5 mL of methanol and 2.5 mL of ultrapure water were respectively loaded, and the sealing cover was covered. The portable reagent storage and liquid adding box can be carried to the site for adding reagents. After extracting the sample to be tested on site, the C18 column needs to be activated first. The portable reagent storage and liquid addition box is rotated and connected with the assembly cover. The sealing cover of the methanol storage chamber is opened, and the sealing insert is pulled out. Methanol passes through the liquid outlet of the storage chamber, the liquid outlet channel, and the funnel through the assembly cover into the solid phase extraction column. When the methanol liquid level is close to the filler height, the sealing cover of the ultrapure water storage chamber is opened, and the sealing insert is pulled out. Ultrapure water passes through the liquid outlet of the storage chamber, the liquid outlet channel, and the funnel through the assembly cover into the solid phase extraction column. When the ultrapure water liquid level is close to the filler height, remove the portable reagent storage and liquid addition box, and directly add about 2.5mL of the sample to be tested to the C18 column through the through hole of the assembly cover. When the liquid level is close to the filler height, replace with a new clean centrifuge tube, add 2.5mL of the sample to be tested again, and collect the filtrate in the centrifuge tube for standby use for the next step of testing.

[0088] Example 6

[0089] The portable handheld solid phase extraction device of Example 1 was used to perform column activation and sample extraction experiments. The solid phase extraction column was a C18 column with a specification of 200 mg / 3 mL, and the outer centrifuge tube was a centrifuge tube with a specification of 50 mL. According to Example 1, the solid phase extraction column, the centrifuge tube and the assembly cover were assembled into one body, and 2.5 mL of methanol was added into the solid phase extraction column from the funnel through hole of the assembly cover. When the methanol liquid level was close to the filler height, 2.5 mL of ultrapure water was added from the funnel through hole of the assembly cover. When the ultrapure water liquid level was close to the filler height, about 2.5 mL of the sample to be tested was added to the C18 column through the through hole of the assembly cover. When the liquid level was close to the filler height, a new clean centrifuge tube was replaced, and 2.5 mL of the sample to be tested was added again. The centrifuge tube collected the filtrate and was used for the next step of detection.

Claims

1. A portable handheld solid phase extraction device, characterized in that The device is composed of a solid phase extraction column, a centrifuge tube, an assembly cover and a portable reagent storage and liquid adding box. The solid phase extraction column is placed in the centrifuge tube, and the upper end of the solid phase extraction column and the inner wall of the assembly cover are rotatably connected; the centrifuge tube mouth is threadedly connected to the lower end of the assembly cover; the assembly cover is a cylindrical shape with an opening at the bottom, and is composed of a top surface and a side wall. The top surface of the assembly cover is provided with a through hole, and the inner surface of the lower end of the side wall is provided with an internal thread that matches the external thread of the centrifuge tube mouth. The inner surface of the side wall is provided with an inwardly protruding groove step above the internal thread and below the lower surface of the top surface, and two groove notches are provided in the middle of the groove step; the two ends of the elliptical protruding step surface of the solid phase extraction column mouth are inserted from the two groove notches, and are screwed into the gap between the upper part of the groove step and the lower surface of the top surface, and are fixed on the groove step, so that the solid phase extraction column and the assembly cover are rotatably connected; The portable reagent storage and liquid-adding box is detachably connected to the assembly cover, and the portable reagent storage and liquid-adding box consists of a cylindrical base and a liquid storage cavity arranged above the base, and the liquid storage cavity is provided with more than one. The liquid storage cavity is a cavity with an upper opening, and a liquid outlet is provided at the bottom of the cavity. The liquid outlet is connected to a liquid outlet channel vertically passing through the base, and the bottom outlet of the liquid outlet channel is provided above the through hole of the assembly cover; the base is provided with a sealing slot perpendicular to the direction of the liquid outlet channel, and the sealing slot penetrates the outer wall of the side of the base at one end toward the outside, and extends into the inner wall of the liquid outlet channel at one end toward the inside, and a sealing insert matching the shape of the sealing slot is provided, and the length of the sealing insert is greater than the sealing slot; the upper end opening of the liquid storage cavity is provided with a sealing cover that can seal the liquid storage cavity.

2. The portable handheld solid phase extraction device according to claim 1, characterized in that The through hole on the top surface of the assembly cover is arranged at the center of the top surface, the outlet of the through hole is located above the tube opening of the solid phase extraction column, and the diameter of the through hole is less than or equal to the tube opening diameter of the solid phase extraction column.

3. The portable handheld solid phase extraction device according to claim 2, characterized in that The through hole on the top surface of the assembly cover is arranged in a funnel shape, the diameter of the bottom outlet of the funnel is less than or equal to the diameter of the tube mouth of the solid phase extraction column, and the diameter of the upper inlet of the funnel is greater than the diameter of the tube mouth of the solid phase extraction column.

4. The portable handheld solid phase extraction device according to claim 1, characterized in that At least one vent hole is arranged at the notch of the slot of the side wall of the assembly cover, or at the side wall portion below the slot step and above the internal thread.

5. The portable handheld solid phase extraction device according to claim 1, characterized in that The detachable connection between the portable reagent storage and liquid-adding box and the assembly cover is a threaded connection, a snap connection or a rotational snap connection.

6. The portable handheld solid phase extraction device according to claim 1, characterized in that On the top surface plane of the assembly cover, at least one groove recessed downward is arranged along the radial direction from the outer edge of the through hole to the outer edge of the top surface.

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