A deep-sea biological sample in-situ chemical fixation device

Abstract

The present invention discloses an in-situ chemical fixation device for deep-sea biological samples, comprising a bottle body, a card cover, a releaser unit and a fixing frame; the two ends of the bottle body are open, the card cover is located at the two openings of the bottle body, the two ends of the rubber rope located in the bottle body are respectively connected to the inner sides of the two card covers, and the outer sides of the card covers are connected through the releaser unit; two fixing frames are arranged in the bottle body, and a mother liquid bag is arranged between the two fixing frames; a bayonet limiting hole is arranged on the fixing frame, and bayonets are arranged in the bayonet limiting holes of the two fixing frames, the pointed end of the bayonet is opposite to the mother liquid bag, and the blunt end of the bayonet extends to the opening of the bottle body. The present invention can be widely applied to a variety of application scenarios, including applications in high-pressure simulation chambers, has a simple structure, is easy to use, and improves the efficiency of chemical fixation of biological samples.

Description

Technical Field

[0001] The present invention belongs to the technical field of deep-sea sampling equipment, and specifically relates to an in-situ chemical fixation device for deep-sea biological samples, which can be applied to the collection and chemical fixation scenarios of biological samples in different water bodies. Background Art

[0002] As life science exploration becomes more sophisticated, it is often necessary to detect the gene expression patterns or chromatin three-dimensional configuration information presented by biological samples in their original environment, which requires in-situ chemical fixation while collecting samples. In the past, the simple and direct sample collection method was to catch biological samples from the deep sea and bring them to the deck, then add chemical reagents for fixation.

[0003] The main problems and shortcomings in the prior art include:

[0004] Since most deep-sea biological samples have been dead for a long time when they are caught on the sea surface, the gene expression in the cells and the three-dimensional structure of chromatin have changed greatly, making it impossible to obtain more scientifically meaningful biological information. In recent years, some technical solutions have emerged to release chemical fixatives by squeezing through the manipulators of submersibles. However, there are very few deep-sea submersibles with a diving depth greater than 1,000 meters and manipulators, which greatly limits the application of this solution. The high-pressure simulation chamber can simulate the high water pressure environment of the deep sea and is often used to study the adaptability of deep-sea organisms to high-pressure environments. In order to obtain scientifically meaningful biological information, in-situ chemical fixation devices are also needed. However, the internal capacity of the high-pressure simulation chamber is limited, and chemical fixation devices that rely on manipulators or submersibles are obviously unusable.

[0005] The significance of solving the above technical problems:

[0006] There is a need for an in-situ chemical fixation device for deep-sea biological samples that can independently release fixatives, so that the biological samples can be collected in the original environment and fixed by chemical reagents at the same time, thereby retaining scientifically significant biological information to the maximum extent possible, and can be widely adapted to a variety of application scenarios, such as independent use, use in a high-pressure chamber, use on a water sampling rack, and use on a manned or unmanned submersible. Summary of the invention

[0007] In view of the above-mentioned problems and shortcomings in the prior art, the present invention provides an in-situ chemical fixation device for deep-sea biological samples, which can be widely used in a variety of application scenarios, including applications in high-pressure simulation chambers. It has a simple structure, is easy to use, and improves the efficiency of chemical fixation of biological samples.

[0008] To this end, the present invention adopts the following technical solutions:

[0009] A deep-sea biological sample in-situ chemical fixation device comprises a bottle body, a card cover, a releaser unit and a fixing frame; the two ends of the bottle body are open, the card cover is located at the two openings of the bottle body, the two ends of a rubber rope located in the bottle body are respectively connected to the inner sides of the two card covers, and the outer sides of the card covers are connected through the releaser unit; two fixing frames are arranged in the bottle body, and a mother liquid bag is arranged between the two fixing frames; the fixing frames are provided with bayonet limiting holes, and bayonets are arranged in the bayonet limiting holes of the two fixing frames, the pointed ends of the bayonets are opposite to the mother liquid bag, and the blunt ends of the bayonets extend to the opening of the bottle body.

[0010] Preferably, the card cover includes a brim plate, one side of the brim plate is provided with an outer handle, the other side is provided with an inner plug, the inner plug is connected to the inner handle, an outer sealing ring is sleeved on the outer periphery of the inner plug, and an inner sealing ring adapted to the outer sealing ring is provided on the inner wall of the opening of the bottle body; an outer handle hole is provided on the outer handle, the outer handle hole is connected to the releaser unit, and an inner handle hole is provided on the inner handle, the inner handle is connected to the rubber rope.

[0011] Preferably, the fixing frame comprises two porous discs, which are connected by supporting columns, and bayonet limiting holes are arranged on the porous discs, and the positions of the bayonet limiting holes of the two porous discs correspond one to one, and the bayonet passes through the two bayonet limiting holes corresponding one to one on the two porous discs, and the two porous discs are both provided with corresponding elongated holes, and the mother liquid bag is provided with a mother liquid bag through hole corresponding to the position of the elongated holes, and the rubber rope passes through the elongated hole and the mother liquid bag through hole.

[0012] Preferably, a magnetic ring is fixed on the bayonet, and the magnetic ring is arranged on the side of the bayonet limiting hole facing the mother liquid bag.

[0013] Preferably, the porous disc is made of magnetic conductive material.

[0014] Preferably, the porous disc is made of stainless steel.

[0015] Preferably, the mother liquid bags include a plurality of bags arranged in parallel.

[0016] Preferably, the mother liquid bag is a disposable item and is made of elastic material.

[0017] Preferably, a water outlet nozzle is provided on the bottle side of the bottle body, and a nozzle cap is provided on the water outlet nozzle; a handle is also provided on the bottle side of the bottle body.

[0018] Preferably, a fixative is provided in the mother liquid bag. After the mother liquid bag is pierced by a bayonet, the fixative in the mother liquid bag is mixed with the seawater in the bottle to form a fixative working solution, which undergoes a desired chemical reaction with the biological sample to complete the in situ chemical fixation of the biological sample.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) The release of the fixative is achieved by relying on the interlocking transmission of the sample bottle's own release unit, bottle cap, built-in fixing frame, bayonet and mother liquid bag, so that the device can independently complete the task of releasing chemical fixatives, and can therefore be widely used in a variety of application scenarios, including applications in high-pressure simulation chambers.

[0021] (2) The release of the fixative in the mother liquid bag is achieved by the impact of the cap with the bayonet, so that the closing of the bottle and the release of the fixative are almost synchronized, the transmission mechanism is simple, and the success rate is high; at the same time, a closed chamber is formed, which is conducive to the role of chemical reagents.

[0022] (3) The fixative is released by rupturing the mother liquid bag. The opening is large and the release speed is fast, which is conducive to rapid in situ fixation.

[0023] (4) The fixative is stored in a mother liquid bag made of soft material, which is conducive to the early discharge of air and is more suitable for deep-sea high-pressure environments.

[0024] (5) Multiple mother liquid bags can be used in parallel, which can avoid premature reactions between certain chemical reagents and is beneficial to preserving the activity of the chemical reagents.

[0025] (6) The mother liquid bag is made of elastic material. After being damaged by a bayonet, the tear is larger and the reagent is released faster.

[0026] (7) The mother liquid bag is a disposable item to avoid cross-contamination.

[0027] (8) The fixing frame can not only be used to limit the sliding direction of the bayonet, but also to fix the bait, attract the aggregation of organisms, and improve the efficiency of chemical fixation of biological samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a right-side structural schematic diagram of an in-situ chemical fixation device for deep-sea biological samples provided by the present invention.

[0029] Figure 2 The diagram is a top view of the structure of an in-situ chemical fixation device for deep-sea biological samples provided by the present invention.

[0030] Figure 3 It is a schematic diagram of the expansion inside the bottle.

[0031] Figure 4 This is a schematic diagram of the structure of a bayonet.

[0032] Figure 5 It is a structural schematic diagram of the fixing frame.

[0033] Explanation of the accompanying drawings: 1. bottle body; 2. card cover; 3. releaser unit; 4. fixing frame; 201. brim plate; 202. inner plug; 203. inner handle; 204. inner handle hole; 205. rubber rope; 206. outer sealing ring; 207 outer handle; 208. outer handle hole; 401. porous disc; 402. supporting column; 403. bayonet limiting hole; 404. bayonet; 405. elongated hole; 406. magnetic ring; 407. mother liquid bag; 408. mother liquid bag through hole; 4041. blunt end; 4042. pointed end. DETAILED DESCRIPTION

[0034] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, wherein the specific embodiments and descriptions are only used to explain the present invention but are not intended to limit the present invention.

[0035] like Figure 1 and Figure 2 As shown, the present invention discloses an in-situ chemical fixation device for deep-sea biological samples, comprising a bottle body 1, a card cover 2, a releaser unit 3 and a fixing frame 4; the two ends of the bottle body 1 are open, the card cover 2 is located at the two openings of the bottle body 1, the two ends of the rubber rope 205 located in the bottle body 1 are respectively connected to the inner sides of the two card covers 2, and the outer sides of the card covers 2 are connected through the releaser unit 3; two fixing frames 4 are arranged in the bottle body 1, and a mother liquid bag 407 is arranged between the two fixing frames 4; a bayonet limiting hole 403 is arranged on the fixing frame 4, and a bayonet 404 is arranged in the bayonet limiting holes 403 of the two fixing frames 4, the pointed end 4042 of the bayonet 404 is opposite to the mother liquid bag 407, and the blunt end 4041 of the bayonet 404 extends to the opening of the bottle body 1.

[0036] Specifically, the card cover 2 includes a brim plate 201, one side of the brim plate 201 is provided with an outer handle 207, and the other side is provided with an inner plug 202, the inner plug 202 is connected to the inner handle 203, the outer periphery of the inner plug 202 is provided with an outer sealing ring 206, and the inner wall of the opening of the bottle body 1 is provided with an inner sealing ring adapted to the outer sealing ring 206; the outer handle 207 is provided with an outer handle hole 208, the outer handle hole 208 is connected to the releaser unit 3, and the inner handle 203 is provided with an inner handle hole 204, and the inner handle hole 204 is connected to the rubber rope 205.

[0037] Specifically, Figure 3 and Figure 5As shown, the fixing frame 4 includes two porous discs 401, which are connected by support columns 402, and are provided with bayonet limiting holes 403. The bayonet limiting holes 403 of the two porous discs 401 correspond in position to each other. The bayonet 404 passes through the two bayonet limiting holes 403 on the two porous discs 401 that correspond in position to each other, and the two porous discs 401 are both provided with corresponding elongated holes 405, and the mother liquid bag 407 is provided with a mother liquid bag through hole 408 corresponding to the position of the elongated hole 405, and the rubber rope 205 passes through the elongated hole 405 and the mother liquid bag through hole 408.

[0038] Specifically, Figure 4 As shown, a magnetic ring 406 is fixed on the bayonet 404 , and the magnetic ring 406 is arranged on the side of the bayonet limiting hole 403 facing the mother liquid bag 407 .

[0039] Specifically, the porous disk 401 is made of magnetic conductive material.

[0040] Specifically, the porous disc 401 is made of stainless steel.

[0041] Specifically, the mother liquid bag 407 includes a plurality of bags arranged in parallel.

[0042] Specifically, the mother liquid bag 407 is a disposable item made of elastic material.

[0043] Specifically, a water spout is provided on the bottle side of the bottle body 1 , and a spout cap is provided on the water spout; a handle is also provided on the bottle side of the bottle body 1 .

[0044] Specifically, the mother liquid bag 407 is provided with a fixative. After the mother liquid bag 407 is pierced by the bayonet 404, the fixative in the mother liquid bag 407 is mixed with the seawater in the bottle 1 to form a fixative working solution, which undergoes a desired chemical reaction with the biological sample to complete the in situ chemical fixation of the biological sample.

[0045] Example

[0046] An in-situ chemical fixation device for deep-sea biological samples comprises a bottle body 1 with openings at both ends, and also comprises card covers 2 arranged at the two openings of the bottle body 1, and the two ends of a rubber rope 205 located in the bottle body 1 are respectively connected to the inner sides of the two card covers 2.

[0047] Two fixing frames 4 are arranged in the bottle body 1, a mother liquid bag 407 is arranged between the two fixing frames 4, a bayonet limiting hole 403 is arranged on the fixing frame 4, a bayonet 404 is arranged in the bayonet limiting holes 403 of the two fixing frames 4, a pointed end 4042 of the bayonet 404 is opposite to the mother liquid bag 407, and a blunt end 4041 of the bayonet 404 extends to the opening of the bottle body 1; a magnetic ring 406 is arranged on the bayonet 404, which can make the bayonet 404 temporarily adsorbed on the fixing frame 4.

[0048] When the card cover 2 is closed, the card cover 2 hits the blunt end 4041 of the bayonet 404, and the bayonet 404 slides along the bayonet limiting hole 403 toward the middle part of the bottle body 1, so that the pointed end 4042 of the bayonet 404 penetrates into the mother liquid bag 407 located in the middle position of the bottle body 1, so that the mother liquid bag 407 ruptures, and the fixative in the mother liquid bag 407 is rapidly released into the bottle body 1 through the rupture, and is mixed with the seawater in the bottle body 1 to form a fixative working solution, thereby undergoing an expected chemical reaction with the biological sample, thereby completing the in situ chemical fixation of the biological sample.

[0049] The fixing frame 4 includes two porous discs 401, which are connected by supporting columns 402. Bayonet limiting holes 403 are provided on the porous discs 401. The bayonet limiting holes 403 of the two porous discs 401 correspond to each other in position. The bayonet 404 passes through the two bayonet limiting holes 403 on the two porous discs 401 that correspond to each other in position. The two porous discs 401 are both provided with corresponding elongated holes 405. The mother liquid bag 407 is provided with a mother liquid bag through hole 408 corresponding to the position of the elongated hole 405. The rubber rope 205 passes through the elongated hole 405 and the mother liquid bag through hole 408.

[0050] A magnetic ring 406 is fixed on the bayonet 404 . The porous disc 401 is made of a magnetic conductive material. The magnetic ring 406 is arranged on the side of the bayonet limiting hole 403 facing the mother liquid bag 407 .

[0051] The bayonet limiting hole 403 limits the bayonet 404, and the bayonet 404 can only move along the axial direction of the bayonet limiting hole 403, so that when the cover 2 hits the blunt end 4041 of the bayonet 404, the bayonet 404 moves along the axial direction of the bayonet limiting hole 403, and the pointed end 4042 of the bayonet 404 will pierce the mother liquid bag 407. When the bayonet 404 is not hit by the cover 2, the bayonet 404 can maintain the relative position relationship with the fixing frame 4 through the magnetic attraction between the magnetic ring 406 and the inner wall of the bayonet limiting hole 403. After the bayonet 404 is hit by the cover 2, the impact force is greater than the magnetic attraction, and the pointed end 4042 of the bayonet 404 pierces the mother liquid bag 407.

[0052] Depending on the actual situation, the usage is different and can be divided into three different application forms: manual form, timing form and trigger form.

[0053] The manual form refers to connecting the releaser unit 3 to a control module on board, on shore, or in a manned submersible, and manually controlling the releaser unit 3 to achieve in-situ chemical fixation of the biological sample.

[0054] The timing form means that a time control program is written in advance in the releaser unit 3 so that it works at the set time, closes the card cover 2, and releases the chemical fixative. If some organisms have already gathered in the bottle body 1 at this time, in-situ chemical fixation can be achieved. This form of use is relatively independent and can also be applied to the use scenario in the high-pressure simulation chamber.

[0055] The triggering mode refers to that a specific signal received by the sensor in the releaser unit 3, such as a signal that a biological substance enters the bottle body 1, etc., automatically triggers the operation of the releaser unit 3, thereby realizing in-situ chemical fixation of the biological sample.

[0056] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An in-situ chemical fixation device for deep-sea biological samples, Features: The invention comprises a bottle body (1), a card cover (2), a releaser unit (3) and a fixing frame (4); the two ends of the bottle body (1) are open, the card cover (2) is located at the two openings of the bottle body (1), the two ends of the rubber rope (205) located in the bottle body (1) are respectively connected to the inner sides of the two card covers (2), and the outer sides of the card covers (2) are connected through the releaser unit (3); two fixing frames (4) are arranged in the bottle body (1), and a mother liquid bag (407) is arranged between the two fixing frames (4); a bayonet limiting hole (403) is arranged on the fixing frame (4), and a bayonet (404) is arranged in the bayonet limiting hole (403) of the two fixing frames (4), the pointed end (4042) of the bayonet (404) is opposite to the mother liquid bag (407), and the blunt end (4041) of the bayonet (404) extends to the opening of the bottle body (1); The mother liquid bag (407) is provided with a fixative. After the mother liquid bag (407) is pierced by the bayonet (404), the fixative in the mother liquid bag (407) is mixed with the seawater in the bottle (1) to form a fixative working solution, which reacts with the biological sample as expected, thereby completing in-situ chemical fixation of the biological sample.

2. The deep-sea biological sample in-situ chemical fixation device according to claim 1, Features: The card cover (2) comprises a brim plate (201), one side of the brim plate (201) is provided with an outer handle (207), and the other side is provided with an inner plug (202), the inner plug (202) is connected to the inner handle (203), an outer sealing ring (206) is sleeved on the outer periphery of the inner plug (202), and an inner sealing ring matched with the outer sealing ring (206) is provided on the inner wall of the opening of the bottle body (1); an outer handle hole (208) is provided on the outer handle (207), and the outer handle hole (208) is connected to the releaser unit (3); an inner handle hole (204) is provided on the inner handle (203), and the inner handle hole (204) is connected to the rubber rope (205).

3. The in-situ chemical fixation device for deep-sea biological samples according to claim 1, Features: The fixing frame (4) comprises two porous discs (401), the porous discs (401) are connected by a supporting column (402), the porous discs (401) are provided with a bayonet limiting hole (403), the positions of the bayonet limiting holes (403) of the two porous discs (401) correspond one to one, the bayonet (404) passes through the two bayonet limiting holes (403) of the two porous discs (401) which correspond one to one, the two porous discs (401) are provided with a corresponding elongated hole (405), the mother liquid bag (407) is provided with a mother liquid bag through hole (408) which corresponds to the position of the elongated hole (405), and the rubber rope (205) passes through the elongated hole (405) and the mother liquid bag through hole (408).

4. The deep-sea biological sample in-situ chemical fixation device according to claim 3, Features: A magnetic ring (406) is fixed on the bayonet (404), and the magnetic ring (406) is arranged on the side of the bayonet limiting hole (403) facing the mother liquid bag (407).

5. The deep-sea biological sample in-situ chemical fixation device according to claim 4, Features: The porous disc (401) is made of magnetic conductive material.

6. The deep-sea biological sample in-situ chemical fixation device according to claim 5, Features: The porous disc (401) is made of stainless steel.

7. The deep-sea biological sample in-situ chemical fixation device according to claim 1, Features: The mother liquid bags (407) include a plurality of bags arranged in parallel.

8. The deep-sea biological sample in-situ chemical fixation device according to claim 1, Features: The mother liquid bag (407) is a disposable item and is made of elastic material.

9. An in-situ chemical fixation device for deep-sea biological samples according to any one of claims 1 to 8, Features: The bottle side of the bottle body (1) is provided with a water outlet nozzle, and the water outlet nozzle is blocked with a nozzle cap; the bottle side of the bottle body (1) is also provided with a handle.

Images