Sampling brush and sampling head suitable for sampling microorganisms on solid surfaces

Abstract

The application discloses a sampling brush and a sampling head suitable for sampling of microorganisms on solid surfaces, which can adapt to sampling of complex surfaces and effectively collect samples on uneven solid surfaces. The sampling brush comprises bristles and a bristle holder. The bristle holder is a hollow structure, and a liquid storage material is arranged in the cavity of the bristle holder. The bristles are arranged on the lower end surface of the bristle holder. The bristles are composed of bristle sheaths and bristle fiber bundles. The bristle fiber bundles extend out of the bristle sheaths by a set length. The bristle sheaths are made of elastic material. The bristle fibers forming the bristle fiber bundles are made of material with water absorption and water transmission functions. One end of the bristles is implanted into the cavity of the bristle holder and contacts the liquid storage material. More than one air hole is arranged on the upper end surface of the bristle holder. In addition, based on the sampling brush, the application further provides a translation sampling head and a rotary sampling head.

Description

Technical Field

[0001] The invention relates to a sampling brush and a sampling head, specifically to a sampling brush and a sampling head suitable for sampling microorganisms on solid surfaces, belonging to microbial detection sampling technology. Background Art

[0002] Conventional solid surface microbial sampling generally uses the surface smearing method. National standards GB4789.17-2003 and GB / T18204.4-2013 specify solid surface microbial sampling methods. Aseptically operate on the solid surface, using a sterile, dry cotton swab, evenly smear the sample back and forth across the appropriate area or region of the solid surface. The swab tip is then cut with sterile scissors and placed in a buffer solution for elution. The eluted solution is the collected solid surface microbial sample.

[0003] However, the method of using cotton swabs for sampling microorganisms on solid surfaces only samples local areas of the solid surface, and the sample collection volume is very limited; at the same time, the sampling specification plate used in this method can only sample samples with flat solid surfaces, and has limitations for samples with potholes and uneven solid surfaces; when the level of pathogenic or viral contamination on the solid surface is low, samples cannot be effectively collected, which will inevitably lead to inaccurate test results. Summary of the Invention

[0004] In view of this, the present invention provides a sampling brush suitable for sampling microorganisms on solid surfaces, which can adapt to sampling on complex surfaces and can also effectively collect samples from samples with potholes and uneven surfaces.

[0005] The sampling brush suitable for sampling microorganisms on solid surfaces comprises: bristles and a bristle bracket;

[0006] The bristle bracket is a hollow structure, and the cavity thereof is filled with liquid storage material;

[0007] Bristles are arranged on the lower end surface of the bristle bracket, wherein the bristles are composed of a bristle fiber bundle wrapped by a bristle outer skin, and the bristle fiber bundle extends out of the bristle outer skin to a set length;

[0008] The outer skin of the bristles is made of elastic material; one end of the bristles is implanted in the cavity of the bristle holder and contacts the liquid storage material;

[0009] One or more vent holes are arranged on the upper end surface of the bristle bracket.

[0010] As a preferred embodiment of the present invention, a quick interface is provided on the bristle bracket.

[0011] As a preferred embodiment of the present invention: the quick interface is a hollow structure, which is connected to the inner cavity of the bristle holder; the top opening of the quick interface serves as a liquid inlet for replenishing liquid into the inner cavity of the bristle holder.

[0012] In addition, the present invention provides a sampling head suitable for sampling microorganisms on solid surfaces, wherein the sampling head is a translational sampling head, comprising: a driving mechanism A, a walking mechanism, and a sampling brush; the sampling brush is the sampling brush defined above;

[0013] The driving mechanism A is used to provide power for the walking mechanism;

[0014] The walking mechanism is used to drive the sampling brush to move within the sampling area;

[0015] The sampling brush is used for sampling microorganisms on a solid surface.

[0016] As a preferred embodiment of the present invention: the driving mechanism A is a driving motor A;

[0017] The walking mechanism includes: an active walking mechanism connected to the driving mechanism A and a passive walking mechanism connected to the active walking mechanism; the active walking mechanism includes: a gear A, a rack A, a walking wheel A, and a guide rail A; the passive walking mechanism includes: a gear B, a rack B, a walking wheel B, and a guide rail B;

[0018] The driving motor A is fixedly supported on the sampling brush fixing frame through a fixed connecting rod A, and the sampling brush is connected to the sampling brush fixing frame; the sampling brush fixing frame is fixedly connected to the connecting shaft A; the two ends of the connecting shaft A are respectively supported on the bracket A and the bracket B;

[0019] The gear A is coaxially fixed to the motor output shaft of the drive motor A, and the motor output shaft is supported on the bracket A through the bearing B. The rack A is meshed with the gear A to form a rack-and-pinion transmission; the guide rail A is fixed to the lower end surface of the rack A; the running wheel A is in rolling engagement with the lower surface of the guide rail A; the running wheel A is supported on one end of the connecting shaft A through a bearing;

[0020] The traveling wheel B is supported on the other end of the connecting shaft A through a bearing; the guide rail B that rolls with the traveling wheel B is fixed to the lower end surface of the rack B; the gear B and the rack B are meshed; the connecting shaft B serves as the gear shaft of the gear B and is supported on the bracket B through a bearing.

[0021] As a preferred embodiment of the present invention, the sampling head further comprises a travel limit assembly, and the travel limit assembly comprises travel limit sensors arranged at both ends of the traveling direction of the traveling mechanism.

[0022] As a preferred embodiment of the present invention, a sampling head cover is installed on the outside of the sampling head, and the bottom of the sampling head cover is open; the sampling head cover is used to support the sampling head during the sampling process.

[0023] In addition, the present invention also provides another sampling head suitable for sampling microorganisms on solid surfaces, wherein the sampling head is a rotary sampling head, comprising: a driving mechanism B and a sampling assembly;

[0024] The sampling assembly includes: a sampling brush bracket and two or more sampling brushes; the sampling brushes are the above-mentioned sampling brushes;

[0025] Two or more sampling brushes are evenly spaced apart along the circumference of the lower end surface of the sampling brush bracket with the axis of the sampling brush bracket as the center;

[0026] The driving mechanism B is used to drive the sampling component to rotate around its axis.

[0027] As a preferred embodiment of the present invention: the driving mechanism B includes: a driving motor B and a motor base;

[0028] The driving motor B is fixed to the motor base through a fixed connecting rod B; the output shaft of the driving motor B extends out through a bearing fixed to the motor base and is connected to the sampling brush bracket.

[0029] As a preferred embodiment of the present invention: the outer cover of the driving motor B is equipped with a motor cover.

[0030] Beneficial effects:

[0031] (1) The sampling brush is composed of a bristle fiber bundle wrapped in an elastic bristle outer skin, which can adapt to the sampling of complex surfaces and can also effectively collect samples from samples with potholes and uneven surfaces.

[0032] (2) The sampling brush has a quick plug-in structure, which makes it convenient for operators to quickly replace the sampling brush according to usage requirements during the sampling process.

[0033] (3) The sampling brush has a liquid storage function inside, which can transport liquid to the bristles of the sampling brush, so that the bristles of the sampling brush can be moistened and the microorganisms can be effectively collected.

[0034] (4) The translational sampling head can sample at a constant rate, and the sampling efficiency at each point in the sampling area is consistent, without missing any samples.

[0035] (5) The rotary sampling head can respond to sudden biosafety incidents and implement emergency large-scale screening sampling. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Schematic diagram of the sampling brush structure in Example 1;

[0037] Figure 2 A partial cross-sectional view of the sampling brush in Example 1;

[0038] Figure 3 This is an enlarged partial cross-sectional view of the sampling brush in Example 1;

[0039] Figure 4 This is a bottom view of the sampling brush in Example 1;

[0040] Figure 5 A top view of the sampling brush in Example 1;

[0041] Figure 6 Schematic diagram of the structure of the translational sampling head in Example 2;

[0042] Figure 7 This is a working direction view of the translational sampling head in Example 2;

[0043] Figure 8 This is a top view of the working direction of the translational sampling head in Example 2;

[0044] Figure 9 This is a schematic diagram of the operation of the translational sampling head in Example 2;

[0045] Figure 10 This is a structural diagram of the rotary sampling head in Example 3;

[0046] Figure 11 This is a layout diagram of the sampling brush of the rotary sampling head in Example 3;

[0047] Figure 12 This is a cross-sectional view of the driving component of the rotary sampling head in Example 3;

[0048] Figure 13 This is a working direction view of the rotary sampling head in Example 3.

[0049] Wherein: 191-bristles, 192-bristle bracket, 193-quick interface, 194-liquid storage material, 195-bristle outer skin, 196-bristle fiber bundle, 197-vent, 198-liquid inlet;

[0050] 1-Bearing A, 2-Connecting shaft A, 3-Bracket A, 4-Stroke limit sensor A, 5-Motor output shaft, 6-Bearing B, 7-Gear A, 8-Drive motor A, 9-Fixed connecting rod A, 10-Support connecting part A, 11-Guide rail B, 12-Bracket B, 13-Gear B, 14-Connecting shaft B, 15-Rack B, 16-Guide rail A, 17-Travel wheel B, 18-Sampling brush fixing bracket, 19-Sampling brush, 20-Support connecting part B, 21-Rack A, 22-Stroke limit sensor B, 23-Travel wheel A; 24-Head cover

[0051] 121 - sampling brush bracket, 122 - driving motor B, 123 - fixed connecting rod B, 124 - motor base, 125 - motor cover. DETAILED DESCRIPTION

[0052] The present invention is described in detail below with reference to the accompanying drawings and embodiments.

[0053] Example 1:

[0054] This embodiment provides a sampling brush suitable for sampling microorganisms on solid surfaces, which can adapt to sampling on complex surfaces and can also effectively collect samples from samples with potholes and uneven surfaces.

[0055] like Figures 1-4 As shown, the sampling brush includes: bristles 191, a bristle holder 192 and a quick interface 193; wherein the bristle holder 192 is a hollow structure, and a liquid storage material 194, such as a sponge material, is installed in its cavity; multiple rows of bristles 191 are arranged in an array on the lower end surface of the bristle holder 191, and the bristles 191 are composed of a bristle outer skin 195 wrapping a bristle fiber bundle 196 (the bristle fiber bundle 196 extends out of the bristle outer skin 195 to a set length); wherein the bristle outer skin 195 is a polymer material with elasticity; the bristle fibers used to form the bristle fiber bundle 196 are materials with water absorption and water transport functions, such as cotton fibers. The elasticity of the bristle outer skin 195 enables the sampler to sample on complex surfaces with undulations on the solid surface. One end of the bristle 191 is implanted in the cavity of the bristle holder 192 and contacts the liquid storage material 194.

[0056] like Figure 5 As shown, the upper end surface of the bristle holder 192 is arranged with vent holes 197, and quick connectors 193 are provided at both ends of the upper end surface. The quick connectors 193 are hollow structures and communicate with the inner cavity of the bristle holder 192. Therefore, the top opening of the quick connector 193 can be used as a liquid inlet 198 for replenishing physiological saline into the inner cavity of the bristle holder. When the sampling brush is infiltrated, physiological saline enters the inner cavity of the bristle holder 192 through the liquid inlet 198 of the quick connector 193. The liquid storage material 194 absorbs the liquid to a balanced state, and at the same time, the bristles 191 also absorb the liquid to a balanced state.

[0057] The physiological saline consumed by the bristles 191 of the sampling brush can be continuously replenished from the liquid storage material 194 containing physiological saline in the inner cavity of the bristle holder 192. The bristle fiber bundle 196 wrapped in the bristle outer skin 195 has a siphon phenomenon on water at the microscopic level, which can make the bristle fiber bundle 196 have the function of absorbing and transporting water; the vents 197 on the bristle holder 192 play a role in balancing the pressure of the inner cavity of the bristle holder 192 and the external atmospheric pressure, eliminating the resistance when the physiological saline is transported from the inner cavity of the bristle holder 192 to the bristle fiber bundle 196, keeping the bristles 191 in a moist state, and realizing the effective collection of microorganisms on the solid surface.

[0058] Example 2:

[0059] Based on the sampling brush in the above embodiment 1, this embodiment provides a translational sampling head, which is an automated sampling mechanism capable of sampling at a constant rate.

[0060] like Figure 6-Figure 9 As shown, the translational sampling head includes: a driving mechanism A, a traveling mechanism, a travel limit assembly, and a sampling assembly A; wherein the driving mechanism A is used to provide power to the traveling mechanism; the traveling mechanism is used to drive the sampling assembly to move within the sampling area; the travel limit assembly is used to limit the travel of the traveling mechanism; and the sampling assembly is used to implement sampling. Specifically:

[0061] The driving mechanism A includes: a driving motor A8, a fixed connecting rod A9 and a motor control signal transmission circuit;

[0062] The traveling mechanism includes: gear A7, rack A21, gear B13, rack B15, traveling wheel A23, guide rail A16, traveling wheel B17, guide rail B16, bracket A3 and bracket B12;

[0063] The travel limit assembly includes: travel limit sensor A4, travel limit sensor B22 and their control and signal transmission circuits;

[0064] The sampling assembly A includes a sampling brush fixing frame 18 and a sampling brush 19 . The sampling brush 19 is the sampling brush in the above-mentioned embodiment 1.

[0065] The overall connection relationship of the translational sampling head is as follows: the driving motor A8 is supported on the fixed connecting rod A9, the fixed connecting rod A9 is fixed on the sampling brush fixing frame 18; the sampling brush fixing frame 18 is fixed on the connecting shaft A2, thereby the driving motor housing, the fixed connecting rod 9, the sampling brush fixing frame 18 and the connecting shaft A2 form a rigid structure.

[0066] Gear A7 is coaxially fixed to the motor output shaft 5 of the drive motor A8. When the drive motor A8 is started, the motor output shaft 5 drives the gear A7 to rotate together; the rack A21 is engaged with the gear A7 to form a gear-rack transmission. Since the gear-rack transmission has a fixed transmission ratio, the gear A7 can drive the walking mechanism to perform uniform linear motion along the length direction of the rack A21. By controlling the speed of the drive motor A8, the walking speed of the walking mechanism can be controlled. When used for large-area rapid sampling, the running speed of the walking mechanism is faster than when used for precise sampling.

[0067] The walking mechanism has two sets of motion mechanisms, which are symmetrical and parallel in space; one set of motion mechanisms is an active motion mechanism, and the other set is a passive motion mechanism. The active motion mechanism is directly connected to the motor output shaft 5, and its assembly relationship is: the guide rail A23 is fixed to the lower end face of the rack A21 by connecting fasteners; the bracket A3 assembles and positions the gear A7 and the walking wheel A23 on the rack A21 and the guide rail A16 respectively through the bearing B6 assembled on the motor output shaft 5 and the bearing A1 assembled on the connecting shaft A2, that is, the motor output shaft 5 is supported on the bracket A3 through the bearing B6 to ensure that the gear A7 and the rack A21 are reliably engaged, and the walking wheel A23 rolls with the lower surface of the guide rail A16, and a strip groove is provided on the lower surface of the guide rail A16. The walking wheel A23 is tightly matched with the strip groove on the guide rail A16 to form a groove-wheel high pair connection structure; the walking wheel A23 is supported on one end of the connecting shaft A2 by a bearing (to ensure that the connecting shaft A2 does not rotate when the walking wheel A23 rolls), and the connecting shaft A2 is supported on the bracket A3 at this end by the bearing A1.

[0068] A passive travel mechanism is provided at the other end of connecting shaft A2. Specifically, rack B15 and guide rail B11 are secured together via fasteners, while gear B13 is mounted on connecting shaft B14, meshing with rack B15. Bracket B12, via bearings mounted on connecting shaft B14 and connecting shaft 2, assembles and positions gear B13 and travel wheel B17 on rack B15 and guide rail B11, respectively. Connecting shaft B14, acting as the gear shaft for gear B13, is supported on bracket B12 via bearings to ensure reliable meshing between gear B13 and rack B15. Travel wheel B17 is supported on the other end of connecting shaft A2 via bearings, which are also supported on bracket B12 via bearings. Travel wheel B17 rolls against the lower surface of guide rail B11, which is provided with a strip groove on the lower surface. This tightly fits the strip groove on guide rail B11, forming a groove-wheel high-pair coupling structure.

[0069] The two ends of the two sets of motion mechanisms in the walking direction are fixed to the support connecting member A10 and the support connecting member B20 through connecting fasteners, thereby forming a walking structure.

[0070] Travel limit sensors are installed at both ends of guide rail A16 or guide rail B11 (at both ends of the walking structure's travel direction) to limit the travel of the walking structure. The trigger signals from the two travel limit sensors control the forward or reverse rotation of drive motor A8. The travel limit sensor located on the side of support connector A10 is called travel limit sensor A4, and the travel limit sensor located on the side of support connector B20 is called travel limit sensor B22. Taking the travel limit sensors installed on guide rail A16 as an example: when the walking mechanism moves to the end of support connector A10, bracket A3 contacts and triggers travel limit sensor A4. Travel limit sensor A4 transmits a trigger signal to the external control unit, which controls the reverse rotation of drive motor A8, causing the walking mechanism to move in a uniform linear motion in the opposite direction. This cycle continues until the walking mechanism moves to the end of support connector B2. When bracket A3 contacts and triggers travel limit sensor B22, travel limit sensor B22 transmits a trigger signal to the external control unit, which controls the forward rotation of drive motor A8. This cycle achieves the reciprocating linear motion of the walking mechanism. In order to ensure that the sampling efficiency of the sampling head is consistent at each point in the sampling area, the sampling head should be moved as evenly as possible to avoid missing any points.

[0071] The sampling brush 19 is detachably connected to the sampling brush holder 18 via a quick-release connector. This removable connection facilitates brush replacement. When the sampling head is in operation, the sampling brush 19 and the travel mechanism perform a reciprocating linear motion, brushing back and forth across the surface of the solid sample to collect microorganisms. Before sampling, the sampling brush 19 needs to be thoroughly soaked in a container of sterile saline.

[0072] like Figure 9 As shown, the sampling head is externally covered with a sampling head cover 24, which has an opening at the bottom and is mounted on the outside of the sampling head from the top. The sampling head cover 24 supports the sampling head during the sampling process. The supporting edges of the sampling head cover 24 (i.e., the left and right opposing edges at the lower end of the sampling head cover 24) are preferably smooth, arc-shaped structures to facilitate movement of the sampling head within the sampling area. The lower end of the sampling brush 19 extends out of the sampling head cover 24 to a set length. The elasticity of the bristle outer skin 195 enables the sampler to sample complex surfaces with a solid surface undulation of Δ, where Δ is the length of the sampling brush 19 extending out of the sampling head cover 24.

[0073] The sampling principle of the translational sampling head is:

[0074] In the representative sample area, the sampling brush 19 with controlled head wetting is repeatedly operated to make the target microorganisms existing on the solid surface, especially the complex solid surface, effectively adhere to the surface of the bristles 191 of the sampling brush 19; Figure 8As shown, the effective sampling area is ultimately formed by the stroke S1 of the sampling brush 19 and the width S2 of the sampling brush during operation (i.e., the width of the sampling brush). The sampling area formed by the stroke S1 and the effective working width S2 of the sampling brush conforms to the sampling area requirements of the national standards GB4789.17-2003 and GB / T18204.4-2013 for solid surface microbial sampling methods.

[0075] By changing the walking speed of the sampling head, large-area rapid and accurate sampling can be achieved.

[0076] The steps for sampling microorganisms on solid surfaces using the translational sampling head are as follows:

[0077] (1) Fix the sampling brush fully soaked in physiological saline on the sampling brush fixing frame 18 through the quick interface I and quick interface II;

[0078] (2) Determine the speed of the drive motor A8 according to the sampling purpose. When used for large-area rapid sampling, set a higher speed; when used for precise sampling, set a lower speed;

[0079] (3) Start the driving mechanism A and control the forward / reverse rotation of the driving motor A8 so that the driving mechanism A drives the sampling brush 19 and the walking mechanism to perform reciprocating linear motion. During the motion, the sampling brush 19 brushes back and forth on the surface of the solid sample to collect microorganisms on the solid surface. This sampling method can sample at a constant rate, and the sampling efficiency at each point in the sampling area is consistent, thus avoiding the problem that the sampling efficiency at each point cannot be consistently guaranteed when the sampling personnel perform manual sampling, and no microorganisms are missed.

[0080] (4) Sample processing: After each sampling brush 19 is wiped, it is immediately removed and placed in a triangular flask or a large test tube containing 50 mL of sterile saline solution and sent for inspection immediately.

[0081] The sampling head should be disinfected after each large-area sampling or precise sampling to prevent secondary contamination.

[0082] Example 3:

[0083] This embodiment provides a rotary sampling head. A sampling head with this structural form can respond to sudden biosafety incidents and implement emergency large-scale screening sampling.

[0084] like Figure 10-13 As shown, the rotary sampling head includes: a driving mechanism and a sampling assembly; to distinguish from Example 2, the driving mechanism in this embodiment is referred to as driving mechanism B; the sampling assembly is referred to as sampling assembly B;

[0085] Sampling assembly B is a rotating component comprising a sampling brush holder 121 and multiple sampling brushes 19 ; the sampling brushes 19 are the same as those described in Example 1. The sampling brush holder 121 is a disc-shaped structure, with multiple sampling brushes 19 evenly spaced circumferentially around the lower end surface of the sampling brush holder 121 , centered about the axis of the sampling brush holder 121 . The sampling brushes 19 are connected to the sampling brush holder 121 via a quick-release connector.

[0086] The driving mechanism B is used to drive the sampling assembly B to rotate. The driving mechanism B includes: a driving motor B122, a motor cover 125 and a motor base 124; the driving motor B122 is fixed on the fixed connecting rod B123, and the fixed connecting rod B123 is fixed on the motor base 124; the output shaft of the driving motor B122 extends out from the back through the bearing fixed on the motor base 124 (that is, the output shaft of the driving motor B122 is supported on the motor base 124 through the bearing) and is connected to the sampling brush bracket 121. When the driving motor B122 is started, the motor output shaft rotates to drive the sampling brush bracket 121 around Figure 10 The axis AB (i.e., the central axis of the sampling brush holder 121) rotates at a constant angular velocity, causing the sampling brush on the sampling brush holder 121 to perform a circular motion across the surface of the sampling area to collect samples. The effective sampling area is defined by the area encompassed by the rotational envelope of the sampling brush and the travel of the sampling head (here, the travel of the sampling head refers to the distance the operator moves the sampling head).

[0087] The outer cover of the driving motor B122 is equipped with a motor cover 125.

[0088] The rotary sampling head can rotate at a constant angular velocity. Due to its multiple sampling brushes, the sampling area is several times larger than that of the sampling head in Example 1, enabling rapid sampling and screening over a large area. This type of sampling head can be used when emergency large-scale screening sampling is required in response to sudden biosafety incidents.

[0089] The sampling principle of the rotary sampling head is:

[0090] In the representative sample area, the sampling brush with controlled head wetting rotates and cooperates with the movement of the sampling head in the sampling area. The target microorganisms on the solid surface, especially the complex solid surface, effectively adhere to the brush bristle surface; through the rotation of the sampling brush and the width of its movement during work, an effective sampling area is finally formed.

[0091] The steps for sampling microorganisms on solid surfaces using a rotary sampling head are:

[0092] (1) Fixing multiple sampling brushes 19 fully soaked in physiological saline on the sampling brush bracket 121 through a quick interface;

[0093] (2) The driving mechanism B is started, and the motor output shaft rotates to drive the sampling brush bracket 121 to rotate at a constant angular velocity. The sampling brushes on the sampling brush bracket 121 perform a circular motion on the surface of the sampling area to perform sampling.

[0094] (3) Sample handling: After wiping, remove the sampling brush immediately and place it in a flask or large test tube containing 50 mL of sterile saline solution and send it for inspection immediately. Disinfect the sampling head after each emergency screening sampling to prevent secondary contamination.

[0095] In summary, the above are only preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A sampling brush suitable for sampling microorganisms on solid surfaces, characterized by: include: Bristles (191) and a bristle holder (192); The bristle bracket (192) is a hollow structure, and a liquid storage material (194) is contained in the cavity thereof; A plurality of rows of bristles (191) are arranged in an array on the lower end surface of the bristle bracket (192), wherein the bristles (191) are composed of a bristle outer skin (195) wrapped with a bristle fiber bundle (196), and the bristle fiber bundle (196) extends out of the bristle outer skin (195) to a set length; The liquid storage material (194) and the bristles (191) absorb liquid to a balanced state; The outer skin of the bristles (195) is made of elastic material; one end of the bristles (191) is implanted in the cavity of the bristle bracket (192) and contacts the liquid storage material (194); One or more vent holes (197) are provided on the upper end surface of the bristle bracket (192); Both ends of the upper end surface of the bristle bracket (192) are provided with quick interfaces (193).

2. The sampling brush suitable for sampling microorganisms on solid surfaces according to claim 1, characterized in that: The quick interface (193) is a hollow structure and is in communication with the inner cavity of the bristle bracket (192); the top opening of the quick interface (193) serves as a liquid inlet (198) for replenishing liquid into the inner cavity of the bristle bracket (192).

3. A sampling head suitable for sampling microorganisms on solid surfaces, characterized by: The sampling head is a translational sampling head, comprising: a driving mechanism A, a walking mechanism and a sampling brush; the sampling brush is the sampling brush according to claim 1 or 2; The driving mechanism A is used to provide power for the walking mechanism; The walking mechanism is used to drive the sampling brush to move within the sampling area; The sampling brush is used for sampling microorganisms on a solid surface.

4. The sampling head for solid surface microbial sampling according to claim 3, characterized in that: The driving mechanism A is a driving motor A (8); The walking mechanism comprises: an active walking mechanism connected to the driving mechanism A and a passive walking mechanism connected to the active walking mechanism; the active walking mechanism comprises: a gear A (7), a rack A (21), a walking wheel A (23), and a guide rail A (16); the passive walking mechanism comprises: a gear B (13), a rack B (15), a walking wheel B (17), and a guide rail B (11); The driving motor A (8) is fixedly supported on the sampling brush fixing frame (18) through a fixed connecting rod A (9), and the sampling brush (19) is connected to the sampling brush fixing frame (18); the sampling brush fixing frame (18) is fixedly connected to the connecting shaft A (2); the two ends of the connecting shaft A (2) are respectively supported on the bracket A (3) and the bracket B (12); The gear A (7) is coaxially fixed to the motor output shaft (5) of the drive motor A (8), and the motor output shaft (5) is supported on the bracket A (3) through the bearing B (6). The rack A (21) is meshed with the gear A (7) to form a rack-and-pinion transmission; the guide rail A (16) is fixed to the lower end surface of the rack A (21); the running wheel A (23) is in rolling engagement with the lower surface of the guide rail A (16); the running wheel A (23) is supported on one end of the connecting shaft A (2) through a bearing; The travel wheel B (17) is supported on the other end of the connecting shaft A (2) through a bearing; the guide rail B (11) that is in rolling engagement with the travel wheel B (17) is fixed to the lower end surface of the rack B (15); the gear B (13) and the rack B (15) are meshed; the connecting shaft B (14) is supported on the bracket B (12) through a bearing as the gear shaft of the gear B (13).

5. The sampling head for solid surface microbial sampling according to claim 3 or 4, characterized in that: The sampling head further includes a travel limit assembly, and the travel limit assembly includes travel limit sensors arranged at both ends of the traveling direction of the traveling mechanism.

6. The sampling head for solid surface microbial sampling according to claim 3 or 4, characterized in that: A sampling head cover (24) is installed on the outside of the sampling head, and the bottom of the sampling head cover (24) is open; the sampling head cover (24) is used to support the sampling head during the sampling process.

7. A sampling head suitable for sampling microorganisms on solid surfaces, characterized by: The sampling head is a rotary sampling head, comprising: a driving mechanism B and a sampling assembly; The sampling assembly comprises: a sampling brush bracket (121) and two or more sampling brushes (19); the sampling brush is the sampling brush according to claim 1 or 2; Two or more sampling brushes (19) are evenly spaced and distributed along the circumferential direction on the lower end surface of the sampling brush bracket (121) with the axis of the sampling brush bracket (121) as the center; The driving mechanism B is used to drive the sampling component to rotate around its axis.

8. The sampling head suitable for sampling microorganisms on solid surfaces according to claim 7, characterized in that: The driving mechanism B comprises: a driving motor B (122) and a motor base (124); The driving motor B (122) is fixed to the motor base (124) via a fixed connecting rod B (123); the output shaft of the driving motor B (122) extends through a bearing fixed to the motor base (124) and is connected to the sampling brush bracket (121).

9. The sampling head for solid surface microbial sampling according to claim 8, characterized in that: The outer cover of the driving motor B (122) is provided with a motor cover (125).

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