A cell smear staining device and a method of using the same

Abstract

The present invention relates to a cell smear staining device and a method for using the same, comprising a plurality of liquid storage tanks, a plurality of solenoid valves, a plurality of liquid spray heads, a conversion disk, a cabinet, a slide box, a rotating mechanism and an operating panel; the plurality of liquid storage tanks are detachably supported on the upper side of the cabinet, the plurality of liquid storage tanks correspond to the plurality of solenoid valves one by one, and each solenoid valve is connected to the corresponding liquid storage tank, the plurality of solenoid valves correspond to the plurality of liquid spray heads one by one, and each liquid spray head is connected to the corresponding solenoid valve, the plurality of liquid spray heads are also connected to the conversion disk, and each liquid spray head is arranged along the circumference, and the conversion disk is rotatably connected to the cabinet; the slide box is rotatably connected to the lower side of the cabinet, the slide box is in the shape of a disk, and the slide box includes a plurality of independent boxes, and the plurality of independent boxes are arranged along the circumferential direction, and each independent box is used to hold a slide. The present invention is used for automatic cell staining, and can realize independent staining of each slide, and when the slide box is reciprocated, the dye on the slide can be spread more evenly.

Description

Technical field:

[0001] The invention relates to the technical field of biological experimental equipment, and in particular to a cell smear staining device and a use method thereof. Background technology:

[0002] In medical examinations, microbiology, cell biology and other experiments are usually performed to facilitate clinical pathogen diagnosis. Before microscopic examination, the cell specimen is smeared on a glass slide, and then a stain is dripped onto the slide. In order to make cell staining efficient, a cell smear staining device is required. In the patent with patent number ZL201811526748.X, the reaction box is composed of a reaction box body and a built-in filling block. The reaction box body is rectangular, and heating grooves are respectively arranged on the outer wall surfaces of the surrounding walls of the reaction box body, and a heating plate is arranged in the heating groove; a reagent injection port is arranged at the bottom corner of the reaction box body; the bottom of the reaction box body is inclined, and a liquid level detection groove is arranged on the side wall corresponding to the bottom of the box body adjacent to the reagent injection port; and a liquid inlet is arranged on the side wall opposite to the liquid level detection groove. When in use, multiple slides are placed in the reaction box body. When the liquid level of the reagent reaches the set position, all slides can be submerged by the reagent. However, this structure can only perform uniform staining on a batch of slides, but cannot achieve independent staining of multiple slides. It is also difficult to stain cells on the slides evenly, and cannot meet usage requirements. Summary of the invention:

[0003] 1. Technical issues to be resolved

[0004] The present invention aims to provide a cell smear staining device and a method of using the same, which are used for automatic cell staining, and solve the problem that the existing cell smear staining device cannot achieve independent staining of multiple slides and is not easy to stain the cells on the slide evenly.

[0005] (II) Technical solution

[0006] To solve the above technical problems, the present invention adopts the following technical solutions: A cell smear staining device comprises a plurality of liquid storage tanks, a plurality of solenoid valves, a plurality of liquid spray heads, a conversion disk, a cabinet, a slide box, a rotating mechanism and an operation panel;

[0007] The plurality of liquid storage tanks are detachably supported on the upper side of the cabinet, the plurality of liquid storage tanks correspond to the plurality of solenoid valves one by one, and each solenoid valve is connected to the corresponding liquid storage tank, the plurality of solenoid valves correspond to the plurality of liquid spray heads one by one, and each liquid spray head is connected to the corresponding solenoid valve, the plurality of liquid spray heads are also connected to the conversion disk, and each liquid spray head is arranged along the circumference, and the conversion disk is rotatably connected to the cabinet;

[0008] The slide box is rotatably connected to the lower side of the cabinet through the rotating mechanism. The slide box is in the shape of a disk and includes a plurality of independent boxes arranged along a circumferential direction. Each independent box is used to hold a slide. The slide box can also achieve a small reciprocating swing in a horizontal plane by controlling the rotation.

[0009] The operation panel is used to control the opening and closing of the plurality of electromagnetic valves, the rotation of the conversion disk, and the rotation and shaking of the slide box.

[0010] By providing the liquid storage box, the electromagnetic valve and the liquid spray head in one-to-one correspondence, the liquid in the liquid storage box can flow to the liquid spray head under the control of the electromagnetic valve, and then the liquid spray head can spray liquid to the glass slide box arranged at the lower side of the cabinet. Since the plurality of liquid spray heads are connected to the conversion disk along the circumference, different liquid spray heads can be switched to spray liquid to the glass slide box after the conversion disk rotates. The plurality of liquid storage boxes can contain a plurality of dyeing liquids of different colors, so as to facilitate the immersion of a plurality of glass slides in different colors. The glass slides in the plurality of independent boxes do not interfere with each other, and the glass slides can be stained separately. After the glass slide box rotates, other independent boxes can be switched to be located below the liquid spray head, so as to realize the independent staining of each glass slide. It is also possible to realize the mixed staining of the glass slides by spraying different dyeing liquids to the glass slide box by the rotation of the conversion disk; when the glass slide box is shaken back and forth, the dyeing liquid on the glass slide can be spread more evenly.

[0011] Furthermore, the slide box also includes a slide rack, which is fixedly connected to the inner wall of the independent box, and the slide rack has a groove and a guide groove. The size of the groove is larger than the size of the slide, and the groove is used to hold the slide. The guide groove is located on the side wall of the groove. The slide can be placed in the groove of the slide rack, and the depth of the groove itself can prevent the slide from falling out. The guide groove can discharge the liquid in the groove into the independent box.

[0012] Furthermore, the glass slide rack also has a plurality of partition bosses, and the plurality of partition bosses are in a strip-shaped structure, and the plurality of partition bosses are distributed in parallel in pairs at the bottom of the groove. Glass slides can be accommodated between two of the partition bosses, and between a partition boss and an inner wall of the groove. Multiple glass slides can be laid flat on the glass slide rack to achieve batch staining of glass slides. The partition bosses can isolate every two glass slides to prevent random movement between the two glass slides.

[0013] Furthermore, the glass slide box also includes a heating pad, which is located below the multiple independent boxes. The heating pad can heat the glass slide to quickly dry the dye on the glass slide.

[0014] Furthermore, the cell smear staining device also includes a catheter and a drainage pipe, one end of the catheter is connected to the solenoid valve, and the other end of the catheter is connected to the spray head, the catheter can transport the dye flowing through the solenoid valve to the spray head, the drainage pipe includes a plurality of liquid collection sections and a liquid discharge section, the plurality of liquid collection sections are evenly connected to the independent box, each liquid collection section is connected to the liquid discharge section, and the drainage pipe can discharge the remaining liquid in the independent box from the independent box.

[0015] Furthermore, valves are respectively provided on the multiple liquid collection sections of the liquid discharge pipe, and the liquid in the independent box can be discharged to the outside after the valves are opened.

[0016] Furthermore, the rotating mechanism includes a motor, a gear assembly, an inner gear ring, and a rotating disk. The motor is fixedly connected to the lower side of the cabinet, and is also connected to the gear assembly. The gear assembly is transmission-connected to the inner gear ring. The rotating disk is a hollow disc-shaped structure. The outer ring of the inner gear ring is fixedly connected to the inner ring of the rotating disk. The slide box is arranged on the rotating disk. The motor drives the gear assembly to rotate, and the gear assembly drives the inner gear ring to rotate, thereby driving the rotating disk to rotate.

[0017] Furthermore, the multiple liquid storage boxes include at least one liquid storage box for storing dye solution, and at least one liquid storage box for storing clean water, which can not only be used for cell staining, but also for rinsing residual dye solution on the slide.

[0018] Furthermore, the liquid storage box is in a stepped shape, and a support frame is provided on the cabinet body. The support frame supports the liquid storage box based on the steps of the liquid storage box. The stepped liquid storage box is easy to support and can be efficiently disassembled.

[0019] The present invention also provides a method for using the cell smear staining device, comprising the following steps:

[0020] Step 1, pouring dye solutions and clean water of different colors into the plurality of liquid storage boxes respectively, and placing one or more glass slides carrying cells independently on the glass slide rack in the independent box;

[0021] Step 2: rotating the conversion disk through the operation panel so that the spray head corresponding to the target dye solution faces a vertical downward direction;

[0022] Step 3, rotating the slide box through the operation panel so that the independent box containing the slides to be stained is located directly below the spray head corresponding to the target dye solution;

[0023] Step 4, controlling the electromagnetic valve corresponding to the target dye solution to open through the operation panel, so that the corresponding liquid spray head sprays the dye solution onto the glass slide to be stained, and at the same time controlling the glass slide box to shake back and forth with a small amplitude so that the dye solution fully contacts the cells on the glass slide, and then controlling the electromagnetic valve to close;

[0024] Step 5: Control the heating pad to generate heat through the operation panel to quickly dry the glass slide, open the valve to allow excess dye in the independent box to flow away, rotate the conversion disk through the operation panel, open the solenoid valve corresponding to clean water, and spray clean water on the glass slide through the liquid spray head to clean the residual dye on the glass slide.

[0025] (III) Beneficial effects

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows: by setting a one-to-one corresponding liquid storage box, a solenoid valve and a liquid spray head, the liquid in the liquid storage box can flow to the liquid spray head under the control of the solenoid valve, and then the liquid spray head sprays liquid to the glass slide box arranged on the lower side of the cabinet, and multiple liquid spray heads are connected to the conversion disk along the circumference, and different liquid spray heads can be switched to spray liquid to the glass slide box. The glass slides in multiple independent boxes do not interfere with each other, and the glass slides can be stained separately. After the glass slide box rotates, other independent boxes can be switched to be located below the liquid spray head, thereby realizing independent staining of each glass slide, and when the glass slide box can be shaken back and forth, the dye on the glass slide can be spread more evenly. Description of the drawings:

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below.

[0028] Figure 1 is a schematic structural diagram of a cell smear staining device according to an embodiment of the present invention;

[0029] Figure 2 is a schematic diagram of a top view of the rotating mechanism according to an embodiment of the present invention;

[0030] Figure 3 is a schematic cross-sectional view of the structure of the slide box according to an embodiment of the present invention;

[0031] Figure 4 is a schematic cross-sectional view of another slide box according to an embodiment of the present invention;

[0032] Figure 5 is a schematic structural diagram of a valve according to an embodiment of the present invention;

[0033] Figure 6 is a schematic structural diagram of a support frame according to an embodiment of the present invention;

[0034] In the figure: 1, liquid storage box; 2, solenoid valve; 3, liquid spray head; 4, conversion disk; 5, cabinet; 6, slide box; 7, rotating mechanism; 8, operation panel; 9, catheter; 10, drainage pipe; 51, support frame; 61, independent box; 62, slide rack; 63, groove; 64, guide groove; 65, separation boss; 66, heating pad; 67, slide; 71, motor; 72, gear assembly; 73, inner gear ring; 74, rotating disk; 101, valve Specific implementation method:

[0035] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0036] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 The cell smear staining device shown comprises a plurality of liquid storage boxes 1, a plurality of solenoid valves 2, a plurality of liquid spray heads 3, a conversion disk 4, a cabinet 5, a slide box 6, a rotating mechanism 7 and an operation panel 8;

[0037] A plurality of liquid storage tanks 1 are detachably supported on the upper side of the cabinet 5, the plurality of liquid storage tanks 1 correspond to the plurality of electromagnetic valves 2 one by one, and each electromagnetic valve 2 is connected to the corresponding liquid storage tank 1, the plurality of electromagnetic valves 2 correspond to the plurality of liquid spray heads 3 one by one, and each liquid spray head 3 is connected to the corresponding electromagnetic valve 2, the plurality of liquid spray heads 3 are also connected to the conversion disk 4, and each liquid spray head 3 is arranged along the circumference, and the conversion disk 4 is rotatably connected to the cabinet 5;

[0038] The slide box 6 is rotatably connected to the lower side of the cabinet 5 through a rotating mechanism 7. The slide box 6 is disc-shaped and includes a plurality of independent boxes 61. The plurality of independent boxes 61 are arranged along the circumferential direction. Each independent box 61 is used to hold a slide. The slide box 6 can also reciprocate in a horizontal plane.

[0039] The operation panel 8 is used to control the opening and closing of the plurality of electromagnetic valves 2 , the rotation of the conversion disk 4 , and the rotation and shaking of the slide box 6 .

[0040] It should be noted that the liquid in the independent box 61 will not interfere with the liquid in other independent boxes 61, so that each slide can be stained independently without interfering with each other. When the disc-shaped slide box 6 rotates and switches other independent boxes 61, other spaces will not be occupied.

[0041] Preferably, the slide box 6 further comprises a slide rack 62, which is fixedly connected to the inner wall of the independent box 61, and has a groove 63 and a guide groove 64. The size of the groove 63 is larger than the size of the slide, and the groove 63 is used to hold the slide 67. The guide groove 64 is located on the side wall of the groove 63, so that the slide 67 can be placed in the groove 63 of the slide rack 62. The depth of the groove 63 itself can prevent the slide 67 from falling out, and the guide groove 64 can discharge the liquid in the groove 63 into the independent box 61. .

[0042] Preferably, the glass slide rack 62 further has a plurality of partition bosses 65, which are in a strip-shaped structure, and are parallelly distributed in pairs at the bottom of the groove 63, and the glass slides 67 can be accommodated between two partition bosses 65 and between the partition bosses 65 and the inner wall of the groove 63. In this way, a plurality of glass slides 67 can be laid flat on the glass slide rack 62, and batch staining of the glass slides 67 can be achieved, and the partition bosses 65 can isolate every two glass slides 67 to prevent the two glass slides 67 from moving randomly.

[0043] Preferably, the slide box 6 further comprises a heating pad 66, and the heating pad 66 is located below the plurality of independent boxes 61. The heating pad 66 can heat the slide to make the dye on the slide dry quickly.

[0044] Preferably, the cell smear staining device further comprises a conduit 9 and a drainage pipe 10, one end of the conduit 9 is connected to the electromagnetic valve 2, and the other end of the conduit 9 is connected to the liquid spray head 3. The conduit 9 can transport the dye solution flowing through the electromagnetic valve 2 to the liquid spray head 3, and the drainage pipe 10 comprises a plurality of liquid collection sections and a liquid discharge section, and the plurality of liquid collection sections are evenly connected to the independent box 61, and each liquid collection section is connected to the liquid discharge section. The drainage pipe 10 can discharge the remaining liquid in the independent box 61 out of the independent box 61.

[0045] Preferably, valves 101 are respectively provided on the multiple liquid collection sections of the liquid discharge pipe, and the liquid in the independent box can be discharged to the outside after the valve 101 is opened.

[0046] Preferably, the rotating mechanism 7 comprises a motor 71, a gear assembly 72, an inner gear ring 73, and a rotating disk 74. The motor 71 is fixedly connected to the lower side of the cabinet 5. The motor 71 is also connected to the gear assembly 72. The gear assembly 72 is in driving connection with the inner gear ring 73. The rotating disk 74 is a hollow disc-shaped structure. The outer ring of the inner gear ring 73 is fixedly connected to the inner ring of the rotating disk 74. The slide box 6 is arranged on the rotating disk 74. A slide rail may be arranged below the rotating disk 74. The rotating disk 74 is slidably connected to the cabinet 5 through the slide rail, or a roller is arranged below the rotating disk 74 so as to be rollingly connected to the cabinet 5. The motor 71 drives the gear assembly 72 to rotate. The gear assembly 72 drives the inner gear ring 73 to rotate, and then drives the rotating disk 74 to rotate. When the motor 71 is controlled to rotate forward and reverse, the slide box 6 can be controlled to rotate back and forth to achieve a reciprocating shaking effect.

[0047] Preferably, the plurality of liquid storage boxes 1 include at least one liquid storage box 1 for storing dye solution, and at least one liquid storage box 1 for storing clean water. In this way, not only can the cells be stained, but also the residual dye solution on the slide can be rinsed.

[0048] Preferably, the liquid storage box 1 is in a stepped shape, and a support frame 51 is provided on the cabinet 5, and the support frame 51 supports the liquid storage box 1 based on the steps of the liquid storage box 1. In this way, the stepped liquid storage box 1 is easy to support and can be efficiently disassembled.

[0049] The method for using the cell smear staining device described in the embodiment of the present invention is as follows:

[0050] When in use, dye solutions and water of different colors are poured into the multiple liquid storage boxes 1, and one or more slides carrying cells are placed independently on the slide rack 62 in the independent box 61; of course, the slides can also be placed on the slide rack 62 by an automatic feeding mechanism; the conversion disk 4 is rotated by the operation panel 8 so that the spray head 3 corresponding to the target dye solution faces the vertical downward direction; the slide box 6 is rotated by the operation panel 8 so that the independent box 61 containing the slide to be stained is located directly below the spray head 3 corresponding to the target dye solution; the operation panel 8 is controlled to rotate the conversion disk 4 corresponding to the target dye solution. The electromagnetic valve 2 corresponding to the standard dye solution is opened, so that the corresponding liquid spray head 3 sprays the dye solution to the glass slide to be stained, and at the same time, the glass slide box 6 is controlled to shake back and forth with a small amplitude, so that the dye solution fully contacts the cells on the glass slide, and the electromagnetic valve 2 is controlled to close when the dye solution is sprayed to 1 ml; the heating pad 66 is controlled to heat up through the operation panel 8 to make the glass slide dry quickly, and the valve 101 is opened to make the excess dye solution in the independent box 61 flow away, and the conversion disk 4 is rotated through the operation panel 8, and the electromagnetic valve 2 corresponding to the clean water is opened, and the residual dye solution on the glass slide is sprayed with clean water through the liquid spray head 3. Finally, the glass slide is taken away by the automatic feeding mechanism. Of course, after the dye solution fully soaks the glass slide for 2 minutes, the conversion disk 4 can also be controlled to rotate, and the liquid spray head 3 corresponding to the dye solution of another color can be turned to face vertically downward, that is, toward the glass slide that has just been stained, and the dye solution of another color is further sprayed on the glass slide. At the same time, the glass slide box 6 shakes back and forth to mix the two colors of dye solution evenly.

[0051] The liquid in the independent box 61 will not interfere with the liquid in other independent boxes 61, thereby achieving independent dyeing without mutual interference.

[0052] In summary, the cell smear staining device and the use method thereof provided by the present invention are used for automatic cell staining, which solves the problem that the existing cell smear staining device cannot achieve independent staining of multiple slides and is not easy to stain the cells on the slide evenly.

[0053] The present invention is described above by way of examples, but the present invention is not limited to the above specific embodiments, and any changes or modifications made based on the present invention belong to the scope of protection claimed by the present invention.

Claims

1. A cell smear staining device, characterized in that: It includes a plurality of liquid storage tanks, a plurality of solenoid valves, a plurality of liquid spray heads, a conversion disk, a cabinet, a slide box, a rotating mechanism and an operation panel; The plurality of liquid storage tanks are detachably supported on the upper side of the cabinet, the plurality of liquid storage tanks correspond to the plurality of solenoid valves one by one, and each solenoid valve is connected to the corresponding liquid storage tank, the plurality of solenoid valves correspond to the plurality of liquid spray heads one by one, and each liquid spray head is connected to the corresponding solenoid valve, the plurality of liquid spray heads are also connected to the conversion disk, and each liquid spray head is arranged along the circumference, and the conversion disk is rotatably connected to the cabinet; The slide box is rotatably connected to the lower side of the cabinet through the rotating mechanism. The slide box is in the shape of a disk and includes a plurality of independent boxes arranged along a circumferential direction. Each independent box is used to hold slides. The slide box can also reciprocate in a horizontal plane. The operation panel is used to control the opening and closing of the plurality of electromagnetic valves, the rotation of the conversion disk, and the rotation and shaking of the slide box.

2. A cell smear staining device according to claim 1, characterized in that: The slide box also includes a slide rack, which is fixedly connected to the inner wall of the independent box. The slide rack has a groove and a guide groove. The size of the groove is larger than the size of the slide. The groove is used to hold the slide, and the guide groove is located on the side wall of the groove.

3. A cell smear staining device according to claim 2, characterized in that: The glass slide rack also has a plurality of partition bosses, which are in strip-shaped structures and are distributed in pairs in parallel at the bottom of the groove. Glass slides can be accommodated between two partition bosses and between a partition boss and the inner wall of the groove.

4. A cell smear staining device according to claim 1, characterized in that: The slide box also includes a heating pad located below the plurality of independent boxes.

5. A cell smear staining device according to claim 1, characterized in that: The cell smear staining device also includes a catheter and a drainage pipe, one end of the catheter is connected to the solenoid valve, and the other end of the catheter is connected to the spray head. The drainage pipe includes a plurality of liquid collection sections and liquid discharge sections. The plurality of liquid collection sections are evenly connected to the independent box, and each liquid collection section is connected to the liquid discharge section.

6. A cell smear staining device according to claim 5, characterized in that: Valves are respectively arranged on the multiple liquid collection sections of the liquid discharge pipe.

7. A cell smear staining device according to claim 1, characterized in that: The rotating mechanism includes a motor, a gear assembly, an inner gear ring, and a rotating disk. The motor is fixedly connected to the lower side of the cabinet, and is also connected to the gear assembly. The gear assembly is drivingly connected to the inner gear ring. The rotating disk is a hollow disc-shaped structure. The outer ring of the inner gear ring is fixedly connected to the inner ring of the rotating disk, and the slide box is arranged on the rotating disk.

8. A cell smear staining device according to claim 1, characterized in that: The multiple liquid storage boxes include at least one liquid storage box for storing dye solution, and at least one liquid storage box for storing clean water.

9. A cell smear staining device according to claim 1, characterized in that: The liquid storage box is in a stepped shape, and a support frame is arranged on the cabinet body, and the support frame supports the liquid storage box based on the steps of the liquid storage box.

10. A method for using a cell smear staining device according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1, pouring dye solutions and clean water of different colors into the plurality of liquid storage boxes respectively, and placing one or more glass slides carrying cells independently on the glass slide rack in the independent box; Step 2: rotating the conversion disk through the operation panel so that the spray head corresponding to the target dye solution faces a vertical downward direction; Step 3, rotating the slide box through the operation panel so that the independent box containing the slides to be stained is located directly below the spray head corresponding to the target dye solution; Step 4, controlling the electromagnetic valve corresponding to the target dye solution to open through the operation panel, so that the corresponding liquid spray head sprays the dye solution onto the glass slide to be stained, and at the same time controlling the glass slide box to shake back and forth with a small amplitude so that the dye solution fully contacts the cells on the glass slide, and then controlling the electromagnetic valve to close; Step 5: Control the heating pad to generate heat through the operation panel to quickly dry the glass slide, open the valve to allow excess dye in the independent box to flow away, rotate the conversion disk through the operation panel, open the solenoid valve corresponding to clean water, and spray clean water on the glass slide through the liquid spray head to clean the residual dye on the glass slide.

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