Cell extraction component and cell extraction instrument

Abstract

The present invention provides a cell extraction component and a cell extractor, which relate to the technical field of cell extraction, and include an injection needle, an experimental cup and a flow guide mechanism; one end of the flow guide mechanism extends into the interior of the experimental cup, the flow guide mechanism is provided with a needle guide section, the injection needle is used to be inserted into the flow guide mechanism, and the end of the injection needle is located in the needle guide section, and the injection needle injects a diluent into the experimental cup through the flow guide mechanism; the needle tip of the injection needle is located in the needle guide section, so that the needle tip does not need to directly enter the internal space of the experimental cup, and the possibility of the needle tip end of the injection needle being contaminated by the test solution in the experimental cup is avoided to the greatest extent, ensuring that each extraction process will not interfere with each other, and ensuring the accuracy of the extraction result; the technical problem that the impact of the injection solution in the cell extraction device in the prior art will cause sample splashing, which is easy to cause contamination of the injection needle, and further affects the reliability of subsequent detection is alleviated.

Description

Technical Field

[0001] The present invention relates to the technical field of cell extraction, and in particular to a cell extraction component and a cell extraction instrument. Background Art

[0002] In criminal cases, it is not uncommon to find biological samples such as blood-stained cigarette butts and gloves left at the scene by suspects, which contain a mixture of human blood and exfoliated epithelial cells. Due to the contamination of the victim's blood, conventional DNA testing is mostly mixed STR (short tandem repeat sequence) typing, or even only a single STR typing of the victim is obtained due to the dominant amplification of human blood DNA, which not only increases the difficulty of result interpretation, but also may lose strong evidence against the suspect.

[0003] In the prior art, the research on cell separation methods for mixed biological samples mainly includes: immunomagnetic bead directional capture, flow cytometry, microscopic capture, laser capture microdissection and microfluidic chip technology. Although the above methods can capture target cells with high precision, they have the problems of cumbersome sample pretreatment, expensive equipment and consumables, complex operation and difficulty in promotion. In addition, microfiltration technology, as a separation technology, plays an important role in medical fields such as leukocyte filtration separation and circulating tumor cell separation and capture with the development of materials science and preparation technology, especially the continuous advancement of precision processing technology.

[0004] The microfiltration technology in the prior art is mostly limited to manual operation, which is labor-intensive and easy to be contaminated. In the cell extraction device for cell extraction, the test cup is used to place the sample (such as epidermal fragments, semen, blood, human tissue residual samples and other amorphous samples) and dissolve it by injecting diluent. The prior art usually uses an injection needle to extend from the upper opening of the test cup into the cup body space and inject liquid. The filtered test cup needs to be cleaned and filtered again to reduce sample loss and maximize the collection of cell samples. However, in this process, since the injection liquid has a certain fluid impact force and inevitably produces sample splashing, it is easy to cause injection needle contamination. Since the injection needle needs to be reused, when it is contaminated, it will seriously affect the reliability of subsequent detection. Summary of the invention

[0005] The purpose of the present invention is to provide a cell extraction component and a cell extractor to alleviate the technical problem that during cell extraction in the cell extraction equipment in the prior art, the impact of the injection liquid will cause sample splashing, which is easy to cause contamination of the injection needle and further affect the reliability of subsequent detection.

[0006] The present invention provides a cell extraction assembly, comprising: an injection needle, a test cup and a flow guide mechanism;

[0007] An inlet is provided at one end of the experimental cup, and one end of the flow guide mechanism is sealed and connected to the inlet so that one end of the flow guide mechanism extends into the interior of the experimental cup. The flow guide mechanism is provided with a needle guide section, and the injection needle is used to be inserted into the flow guide mechanism, and the end of the injection needle is located in the needle guide section. The injection needle injects the diluent into the experimental cup through the flow guide mechanism.

[0008] In a preferred embodiment of the present invention, the experimental cup comprises a cup body and a cup cover;

[0009] The experimental cup is provided with an open end, the cup cover is sealed and connected to the open end of the experimental cup, the inlet is located at the center of the cup cover, and the needle guide section of the diversion mechanism is sealed and connected to the cup cover.

[0010] In a preferred embodiment of the present invention, the flow guiding mechanism further comprises a liquid flow guiding section;

[0011] The liquid guiding section is connected to the needle guiding section, and the liquid guiding section extends into the cup body. The liquid guiding section is used to allow the liquid transported by the injection needle to flow into the cup body through the needle guiding section.

[0012] In a preferred embodiment of the present invention, the liquid guiding section is a tubular structure, and the length of the internal flow channel of the liquid guiding section is greater than the inner diameter of the internal flow channel of the liquid guiding section.

[0013] In a preferred embodiment of the present invention, the liquid guiding section is inclined along the extension direction.

[0014] In a preferred embodiment of the present invention, the liquid guiding section has a spiral structure along the extension direction.

[0015] In a preferred embodiment of the present invention, the needle guide section gradually shrinks along the extension direction toward the cup cover, so that the needle guide section has an inverted frustum structure.

[0016] In a preferred embodiment of the present invention, the flow guiding mechanism further comprises a self-sealing membrane and a self-sealing structure;

[0017] The self-sealing film is located at one end of the needle guide section away from the cup body, the self-sealing film is sealed and connected to the needle guide section, and the injection needle is used to pass through the self-sealing film and extend into the needle guide section;

[0018] The self-sealing structure is evenly arranged along the circumferential direction of the inner wall of the needle guide section. The self-sealing structure is provided with a clamping channel, and the injection needle is used to extend into the clamping channel.

[0019] In a preferred embodiment of the present invention, it also includes a filtering mechanism;

[0020] The filtering mechanism includes a filter and a cell collector; the end of the experimental cup away from the inlet is connected to the cell collector through the filter, the filter and the cell collector are used to filter the mixture of the cell sample and the diluent in the experimental cup in turn, and the cell collector is used to extract DNA typing from the cell sample.

[0021] The present invention provides a cell extractor, comprising the cell extraction component.

[0022] The present invention provides a cell extraction component, comprising: an injection needle, an experimental cup and a flow guide mechanism; an inlet is provided at one end of the experimental cup, and one end of the flow guide mechanism is sealed and connected to the inlet so that one end of the flow guide mechanism extends into the interior of the experimental cup; the flow guide mechanism is provided with a needle guide section, the injection needle is used to be inserted into the flow guide mechanism, and the end of the injection needle is located in the needle guide section, and the injection needle injects a diluent into the experimental cup through the flow guide mechanism; the needle tip of the injection needle is located in the needle guide section, so that the needle tip does not need to directly enter the internal space of the experimental cup, and the possibility of the needle tip end of the injection needle being contaminated by the test solution in the experimental cup is avoided to the greatest extent, ensuring that each extraction process will not interfere with each other, and ensuring the accuracy of the extraction result; the technical problem that the impact of the injection solution in the cell extraction device in the prior art will cause sample splashing, which is easy to cause contamination of the injection needle, and thus affect the reliability of subsequent detection is alleviated. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 A schematic diagram of the external structure of a cell extraction component provided in an embodiment of the present invention;

[0025] Figure 2 A schematic structural diagram of a needle guide section of a cell extraction assembly provided in an embodiment of the present invention;

[0026] Figure 3 A schematic diagram of the overall structure of a liquid guide section of a cell extraction assembly provided in an embodiment of the present invention having a spiral structure;

[0027] Figure 4 A schematic diagram of the overall structure of the liquid guide section of the cell extraction assembly provided by an embodiment of the present invention having an inclined structure;

[0028] Figure 5 A schematic diagram of the structure of an experimental cup of a cell extraction assembly provided in an embodiment of the present invention.

[0029] Icons: 100-injection needle; 200-experimental cup; 201-cup body; 202-cup cover; 203-filtration mechanism; 213-filter; 223-cell collector; 300-guiding mechanism; 301-needle guide section; 302-liquid guiding section; 303-self-sealing membrane; 304-self-sealing structure. DETAILED DESCRIPTION

[0030] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] like Figure 1-Figure 5 As shown, a cell extraction component provided in this embodiment includes: an injection needle 100, an experimental cup 200 and a flow guide mechanism 300; an inlet is provided at one end of the experimental cup 200, and one end of the flow guide mechanism 300 is sealed and connected to the inlet so that one end of the flow guide mechanism 300 extends into the interior of the experimental cup 200, and the flow guide mechanism 300 is provided with a needle guide section 301, the injection needle 100 is used to be inserted into the flow guide mechanism 300, and the end of the injection needle 100 is located in the needle guide section 301, and the injection needle 100 injects the diluent into the experimental cup 200 through the flow guide mechanism 300.

[0032] It should be noted that the cell extraction component provided in this embodiment is provided with a flow guiding mechanism 300 at the entrance position of the experimental cup 200, wherein the needle position of the injection needle 100 extends into the inside of the flow guiding mechanism 300, and the end of the injection needle 100 is located in the needle guiding section 301. When the injection needle 100 injects the diluent into the experimental cup 200, the diluent will enter the experimental cup 200 along the needle guiding section 301. Since the injection needle 100 does not enter the experimental cup 200, even if the diluent flushes and splashes the cell sample in the experimental cup 200, the cell sample will not contaminate the injection needle 100 at this time, thereby ensuring the reliability of subsequent detection when the injection needle 100 injects different experimental cups 200.

[0033] The present embodiment provides a cell extraction assembly, comprising: an injection needle 100, a test cup 200 and a flow guide mechanism 300; an inlet is provided at one end of the test cup 200, and one end of the flow guide mechanism 300 is sealed and connected to the inlet so that one end of the flow guide mechanism 300 extends into the inside of the test cup 200, and the flow guide mechanism 300 is provided with a needle guide section 301, the injection needle 100 is used to be inserted into the flow guide mechanism 300, and the end of the injection needle 100 is located in the needle guide section 301, and the injection needle 100 is injected into the test cup 200 through the flow guide mechanism 300. diluent; the needle tip of the injection needle 100 is located in the needle guide section 301, so that the needle tip does not have to directly enter the internal space of the experimental cup 200, and the possibility of the needle tip of the injection needle 100 being contaminated by the test solution in the experimental cup 200 is avoided to the greatest extent, ensuring that each extraction process will not interfere with each other, and ensuring the accuracy of the extraction result; it alleviates the technical problem that in the cell extraction device in the prior art, the impact of the injection liquid will cause sample splashing during cell extraction, which is easy to cause contamination of the injection needle 100, and thus affects the reliability of subsequent detection.

[0034] On the basis of the above embodiments, further, in a preferred embodiment of the present invention, the experimental cup 200 includes a cup body 201 and a cup cover 202; the experimental cup 200 is provided with an open end, the cup cover 202 is sealed connected to the open end of the experimental cup 200, the inlet is located at the center of the cup cover 202, and the needle guide section 301 of the diversion mechanism 300 is sealed connected to the cup cover 202.

[0035] In this embodiment, the cup cover 202 can ensure the sealing of the open cup body 201, and an entrance can be set on the cup cover 202, through which the flow guide mechanism 300 can be connected to the cup cover 202, and the injection needle 100 can be inserted into the cup body 201 through the flow guide mechanism 300; in addition, the cup cover 202 can also be provided with a cell loading hole, through which the cell loading hole can be used to manually load the cell sample into the cup body 201 on the basis of having a diluent in the cup body 201.

[0036] In a preferred embodiment of the present invention, the guide mechanism 300 also includes a liquid guide section 302; the liquid guide section 302 is connected to the needle guide section 301, and the liquid guide section 302 extends into the cup body 201. The liquid guide section 302 is used to direct the liquid transported by the injection needle 100 into the cup body 201 through the needle guide section 301.

[0037] In this embodiment, the needle guide section 301 can accommodate the needle part of the injection needle 100, and is connected to the needle guide section 301 by the liquid guide section 302. When the injection needle 100 injects the diluent into the needle guide section 301, the liquid guide section 302 is used to slowly guide the diluent into the experimental cup 200.

[0038] In a preferred embodiment of the present invention, the liquid guiding section 302 is a tubular structure, and the length of the internal flow channel of the liquid guiding section 302 is greater than the inner diameter of the internal flow channel of the liquid guiding section 302 .

[0039] In this embodiment, the length of the internal flow channel of the liquid guide section 302 is greater than the inner diameter of the internal flow channel of the liquid guide section 302. Preferably, the length of the internal flow channel of the liquid guide section 302 is more than twice the inner diameter of the internal flow channel of the body guide section, so that the liquid guide section 302 can slowly guide the diluent in the extension direction, thereby ensuring the injection speed of the diluent into the experimental cup 200 and reducing the impact of the diluent entering the experimental cup 200 on the cell sample inside the experimental cup 200.

[0040] Optionally, the liquid guide section 302 can be arranged in multiple ways. In a preferred embodiment of the present invention, the liquid guide section 302 is inclined along the extension direction. By arranging the liquid guide section 302 in an inclined structure, when the diluent enters the interior of the experimental cup 200, the diluent will directly contact the side wall of the experimental cup 200. Since the bottom of the experimental cup 200 is connected to the filter 213 described below, it is ensured that the diluent will not directly impact the filter membrane inside the filter 213. In addition, the diluent will not directly impact the cell sample located at the bottom of the experimental cup 200, thereby preventing the cell sample from splashing, making the design more reasonable.

[0041] In a preferred embodiment of the present invention, the liquid guide section 302 has a spiral structure along the extension direction. In this embodiment, by adopting the liquid guide section 302 with a spiral structure, when the diluent enters the liquid guide section 302 through the needle guide section 301, the diluent will gradually slow down the conveying speed in the liquid guide section 302, so that the diluent slowly falls into the cup body 201, avoiding the possibility of sample splashing caused by the diluent entering the cup body 201 and impacting it.

[0042] In a preferred embodiment of the present invention, the needle guide section 301 gradually shrinks along the extension direction toward the cup cover 202, so that the needle guide section 301 presents an inverted frustum structure.

[0043] In this embodiment, the needle guide section 301 has an inverted frustum structure that gradually narrows in the direction extending toward the main body of the cup cover 202, so as to guide the needle of the injection needle 100 into the interior thereof and improve the fault tolerance effect when the injection needle 100 is aligned.

[0044] In a preferred embodiment of the present invention, the flow guiding mechanism 300 also includes a self-sealing film 303 and a self-sealing structure 304; the self-sealing film 303 is located at one end of the needle guide section away from the cup body 201, the self-sealing film 303 is sealed and connected to the needle guide section 301, and the injection needle 100 is used to pass through the self-sealing film 303 and extend into the needle guide section 301; the self-sealing structure 304 is evenly arranged along the circumferential direction of the inner wall of the needle guide section 301, and the self-sealing structure 304 is provided with a clamping channel, and the injection needle 100 is used to extend into the clamping channel.

[0045] In this embodiment, the internal space of the needle guide section 301 has a self-sealing self-sealing structure 304, wherein the self-sealing structure 304 can adopt an expandable structure. When the needle of the injection needle 100 does not enter the self-sealing structure 304, it can automatically block the internal and external spaces of the cup cover 202; when the injection needle 100 extends into the self-sealing structure 304 along the clamping channel, the self-sealing structure 304 is opened at this time; optionally, the self-sealing structure 304 can adopt a rubber sealing ring.

[0046] In a preferred embodiment of the present invention, a filtering mechanism 203 is also included; the filtering mechanism 203 includes a filter 213 and a cell collector 223; the end of the experimental cup 200 away from the entrance is connected to the cell collector 223 through the filter 213, the filter 213 and the cell collector 223 are used to filter the mixture of the cell sample and the diluent in the experimental cup 200 in turn, and the cell collector 223 is used to extract DNA typing in the cell sample.

[0047] Optionally, the experimental cup 200 can adopt a structure that can accommodate 30 ml of diluent, and the wall surface of the experimental cup 200 can be smoothed to effectively avoid wall sticking; wherein, the experimental cup 200 and the filter 213 can be sealed connected by a Luer connector, and the filter 213 and the cell collector 223 can also be sealed connected by a Luer connector; wherein, the experimental cup 200 is one of the core components of the cell separation test and is a disposable consumable.

[0048] In a preferred embodiment of the present invention, a first filter membrane is provided in the filter 213, and the pore size of the first filter membrane is larger than the DNA typing in the cell sample. A second filter membrane is provided in the cell collector 223, and the pore size of the second filter membrane is smaller than the pore size of the first filter membrane. The second filter membrane is used to block the DNA typing in the cell sample. In this embodiment, the filter 213 and the cell collector 223 can filter the inside of the experimental cup 200 in sequence respectively, wherein the filter 213 is provided with a first filter membrane, and the cell collector 223 is provided with a second filter membrane. The first filter membrane can block the cell sample with a particle size larger than the high-quality sample in the cell sample, and filter out the high-quality sample from the cell sample that can be used to obtain the DNA typing. When the high-quality sample passes through the second filter membrane, the second filter membrane can collect the high-quality sample; after multiple flushing and filtration of the diluent, a high-quality sample can be obtained on the second filter membrane for downstream analysis and obtaining clear results, and finally the accurate DNA typing of the cell (single STR typing of the cell source) can be obtained.

[0049] The present embodiment provides a cell extractor, including the cell extraction component. Since the technical effect of the cell extractor provided by the present embodiment is the same as the technical effect of the cell extraction component provided by the above embodiment, it will not be described in detail here.

[0050] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cell extraction component, characterized in that: include: Injection needle, experimental cup and diversion mechanism; An inlet is provided at one end of the experimental cup, and one end of the flow guide mechanism is sealed and connected to the inlet so that one end of the flow guide mechanism extends into the interior of the experimental cup. The flow guide mechanism is provided with a needle guide section, and the injection needle is used to be inserted into the flow guide mechanism, and the end of the injection needle is located in the needle guide section. The injection needle injects the diluent into the experimental cup through the flow guide mechanism; The experimental cup comprises a cup body and a cup cover; The experimental cup is provided with an open end, the cup cover is sealed and connected to the open end of the experimental cup, the inlet is located at the center of the cup cover, and the needle guide section of the flow guide mechanism is sealed and connected to the cup cover; The flow guiding mechanism further comprises a liquid flow guiding section; The liquid guiding section is connected to the needle guiding section, the liquid guiding section extends into the cup body, and the liquid guiding section is used to flow the liquid transported by the injection needle into the cup body through the needle guiding section; The needle guide section gradually contracts along an extension direction toward the cup cover, so that the needle guide section is an inverted frustum structure.

2. The cell extraction assembly according to claim 1, characterized in that: The liquid guiding section is in a tubular structure, and the length of the internal flow channel of the liquid guiding section is greater than the inner diameter of the internal flow channel of the liquid guiding section.

3. The cell extraction assembly according to claim 2, characterized in that: The liquid guiding section is in an inclined structure along the extension direction.

4. The cell extraction assembly according to claim 2, characterized in that: The liquid guiding section has a spiral structure along the extension direction.

5. The cell extraction assembly according to claim 1, characterized in that: The flow guiding mechanism also includes a self-sealing film and a self-sealing structure; The self-sealing film is located at one end of the needle guide section away from the cup body, the self-sealing film is sealed and connected to the needle guide section, and the injection needle is used to pass through the self-sealing film and extend into the needle guide section; The self-sealing structure is evenly arranged along the circumferential direction of the inner wall of the needle guide section. The self-sealing structure is provided with a clamping channel, and the injection needle is used to extend into the clamping channel.

6. The cell extraction assembly according to any one of claims 1 to 5, characterized in that: Also includes a filtering mechanism; The filtering mechanism includes a filter and a cell collector; the end of the experimental cup away from the inlet is connected to the cell collector through the filter, the filter and the cell collector are used to filter the mixed solution of the cell sample and the diluent in the experimental cup in turn, and the cell collector is used to extract DNA typing from the cell sample.

7. A cell extractor, characterized in that: The method comprises the cell extraction component according to any one of claims 1 to 6.

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