Heating device for experimental container and biological sample preparation device

Abstract

An embodiment of the present invention provides a heating device, comprising a clamp-like device, the inner surface of which matches the outer surface of an experimental container and is used to clamp or release the experimental container; at least one heating circuit is provided on the inner surface of the clamp-like device; wherein the clamp-like device comprises a body, a rotating shaft, a first arm, and a second arm; the body forms a cavity surrounding the experimental container; the rotating shaft is provided on the body; the first arm and the second arm use the rotating shaft as a fulcrum to maintain or open the body. The inner surface of the clamp-like device of the embodiment of the present invention is provided with a heating circuit, so that the clamp-like device can heat the experimental container when clamping the experimental container; the body of the clamp-like device forms a cavity surrounding the experimental container; the first arm and the second arm of the clamp-like device use the rotating shaft provided on the body as a fulcrum, and use the principle of leverage to open or maintain the body of the clamp-like device, making it easy to load and disassemble without affecting other operations in the experimental container.

Description

Technical Field

[0001] The present invention relates to the technical field of laboratory instruments, and in particular to a heating device for an experimental container and a biological sample preparation device. Background Art

[0002] In many biological experiments, it is often necessary to simulate the body temperature of an organism or maintain a specific temperature during the preparation of biological samples to facilitate the experiment. Therefore, a heating device is needed that can heat a single experimental container without affecting other experimental operations. For example, when preparing single-cell suspensions, operations such as cutting and grinding tissue are performed in the experimental container, and the biological sample in each experimental container needs to be maintained at a different specific temperature. Summary of the Invention

[0003] An embodiment of the present invention provides a heating device for an experimental container, aiming to solve the problem of independent heating of different experimental containers in the prior art.

[0004] In a first aspect, a heating device for an experimental container is provided, comprising:

[0005] A clamp-shaped device (1), the inner surface of the clamp-shaped device (1) matches the outer surface of the experimental container, and is used to clamp the experimental container or release the experimental container;

[0006] at least one heating circuit (2), the heating circuit (2) being arranged on the inner surface of the clamp-shaped device (1);

[0007] The clamp-like device (1) comprises a body (11), a rotating shaft (12), a first arm (13) and a second arm (14); the body (11) forms a cavity surrounding the experimental container; the rotating shaft (12) is arranged on the body (11); the first arm (13) and the second arm (14) use the rotating shaft (12) as a fulcrum to maintain or expand the body (11).

[0008] In a second aspect, a biological sample preparation device is provided, comprising a housing, a plurality of motors, a plurality of experimental containers, and a plurality of heating devices as described above;

[0009] A plurality of motors are arranged in the shell, and the driving shafts of the plurality of motors are respectively connected to the plurality of experimental containers to drive the plurality of experimental containers to rotate, and the plurality of heating devices respectively clamp the plurality of experimental containers for heating.

[0010] The inner surface of the clamp-like device of the embodiment of the present invention is provided with a heating circuit, so that the experimental container can be heated when the clamp-like device clamps the experimental container. The main body of the clamp-like device forms a cavity surrounding the experimental container. The first arm and the second arm of the clamp-like device use the rotating shaft provided on the main body as a fulcrum, and utilize the lever principle to support or maintain the main body of the clamp-like device, which is easy to load and disassemble and will not affect other operations in the experimental container. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the following description of the embodiments in conjunction with the accompanying drawings, in which:

[0012] Figure 1 is a schematic diagram of a heating device provided in Example 1 of the present invention;

[0013] Figure 2 is a schematic diagram of an experimental container provided in Example 1 of the present invention;

[0014] Figure 3 This is a bottom view of the heating device provided in Example 1 of the present invention;

[0015] Figure 4a 1 is a schematic diagram of the clamp-shaped device 1 provided in the first embodiment of the present invention in a maintaining state;

[0016] Figure 4b 1 is a schematic diagram of the clamp-shaped device 1 provided in the first embodiment of the present invention in an expanded state;

[0017] Figure 5 Schematic diagram of a biological sample preparation device provided in the second embodiment of the present invention. DETAILED DESCRIPTION

[0018] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar modules or modules with the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention and are not to be construed as limiting the present invention. On the contrary, the embodiments of the present invention include all variations, modifications and equivalents that fall within the spirit and scope of the appended claims.

[0019] Tissue is a cellular structure between cells and organs, composed of many morphologically similar cells and intercellular matrix. In order to conduct cellular research on organisms, the tissue needs to be processed and prepared into a single-cell suspension. A single-cell suspension is a biological sample in which the connections between cells in the tissue are destroyed, releasing dispersed individual cells. The prepared single-cell suspension must have the characteristics of sufficient cell dispersion and high activity. In other words, the connections between cells must be fully destroyed while maintaining the integrity and activity of individual cells. This is the basis for cell research. During the process of destroying the connections between cells in the tissue in the experimental container, the biological sample in the experimental container needs to be maintained at a specific temperature, such as 37 degrees Celsius.

[0020] The inner surface of the clamp-like device of the embodiment of the present invention is provided with a heating circuit, so that the experimental container can be heated when the clamp-like device clamps the experimental container. The main body of the clamp-like device forms a cavity surrounding the experimental container. The first arm and the second arm of the clamp-like device use the rotating shaft provided on the main body as a fulcrum, and utilize the lever principle to support or maintain the main body of the clamp-like device, which is easy to load and disassemble and will not affect other operations in the experimental container.

[0021] Example 1

[0022] Figure 1 Schematic diagram of a heating device provided in Example 1 of the present invention. Figure 2 Schematic diagram of the experimental container provided in Example 1 of the present invention. Figure 3 This is a bottom view of the heating device provided by the first embodiment of the present invention. Figure 1 、 Figure 2 and Figure 3 , the heating device comprises a clamp-like device 1 and at least one heating circuit 2.

[0023] The experimental container for preparing a single-cell suspension is similar to a cylindrical test tube and consists of an experimental container body and a lid. The experimental container body and the lid are connected together by threads. The clamp-like device 1 forms a cavity around the experimental container, and its inner surface matches the outer surface of the experimental container. The experimental container is placed in the cavity formed by the clamp-like device 1, and the size of the cavity is adjusted. The clamp-like device 1 can clamp and release the experimental container. At least one heating circuit 2 is a resistance heating circuit, which is arranged on the inner surface of the clamp-like device 1. When the clamp-like device 1 clamps the experimental container, the heating circuit 2 is close to the outer surface of the experimental container and can heat the experimental container. The heating circuit 2 can be battery-powered or powered by an external power supply. The heating circuit 2 extends from the inner surface of the clamp-like device 1 to the bottom of the clamp-like device 1, and a contact is formed at the bottom to connect to an external power supply.

[0024] In the embodiment of the present invention, the clamp-shaped device 1 includes a body 11 , a rotation shaft 12 , a first arm 13 , a second arm 14 and a baffle 15 .

[0025] The body 11 forms a cavity surrounding the experimental container. The inner surface of the body 11 serves as the inner surface of the clamp-like device 1 and can match the shape of the outer surface of the experimental container. When the experimental container is a cylindrical test tube, the cross-section of the body 11 is a circular ring that extends vertically for a certain length; alternatively, the cross-section of the body 11 is formed by two semicircular rings that extend vertically for a certain length. To enable clamping and releasing the experimental container, the size of the cavity formed by the body 11 is adjustable. In one embodiment of the present invention, when the cross-section of the body 11 is a circular ring, the body 11 has a vertical gap, also known as an opening. In another embodiment of the present invention, when the cross-section of the body 11 is formed by two semicircular rings, a gap, also known as an opening, is formed at one of the adjacent portions of the semicircular rings, and the other adjacent portion is connected by a movable structure. The structure consisting of the rotating shaft 12, the first arm 13, and the second arm 14 supports and maintains the body 11, allowing the size of the cavity formed by the body 11 to be adjustable. Maintaining the body 11 means that the body 11 is in a non-expanded and non-deformed state. When the experimental container needs to be heated, the main body 11 is opened, the experimental container is loaded, and then released to heat it; when the experimental container does not need to be heated, the main body 11 is opened and the experimental container is unloaded.

[0026] Figure 4a It is a schematic diagram of the clamp-shaped device 1 provided in the first embodiment of the present invention in a maintaining state. Figure 4b Schematic diagram of the clamp-like device 1 provided in the first embodiment of the present invention in an open position. In this embodiment of the present invention, a rotating shaft 12 is disposed on the body 11, extending through the inner and outer surfaces of the body 11. The first arm 13 and the second arm 14 use the rotating shaft 12 as a fulcrum to open or maintain the body 11. When the cross-section of the body 11 is a circular ring or two semi-circular rings, the rotating shaft 12 is disposed at a predetermined position near the opening of the body 11. The first arm 13 and the second arm 14 are respectively connected to the rotating shaft 12. The first arm 13 extends from the rotating shaft 12 out of the top of the body 11, and the second arm 14 extends from the rotating shaft 12 to the opening of the body 11. The first arm 13 and the second arm 14 form a lever with the rotating shaft 12 as the fulcrum. When an operator applies force to the first arm 13, the first arm 13 rotates in one direction, driving the second arm 14 to rotate in the same direction. The second arm 14 extends from the opening of the body 11, opening the body 11, thereby enlarging the cavity formed by the body 11 and releasing the experimental container. When first arm 13 rotates in the opposite direction, it drives second arm 14 to rotate in the opposite direction. Second arm 14 retracts at the opening of body 11, narrowing the opening. Body 11 then recovers, and the resulting cavity becomes smaller, clamping the experimental container. In another embodiment of the present invention, rotation axis 12 is accommodated between the inner and outer surfaces of body 11.

[0027] In the above embodiment, the rotation axis 12 is disposed on the body 11. The vertical position of the rotation axis 12 relative to the body 11 is not limited. The position of the rotation axis 12 serves as the fulcrum of the lever, and the end of the second arm 14, distal from the rotation axis 12, serves as the prying point. This prying point can be located in the middle or bottom of the opening of the body 11, ensuring more uniform deformation of the entire body 11, extending its service life and reducing manufacturing requirements.

[0028] In an embodiment of the present invention, preferably, the length of the first arm 13 is greater than the length of the second arm 14, so that the operator can use less force on the first arm 13. The baffle 15 is provided at the top of the body 11, at a preset position near the first arm 13. When the operator applies force to the first arm 13, the baffle 15 limits the first arm 13 to prevent damage caused by excessive operation by the operator. Furthermore, the heating device also includes an elastic sleeve 3 mounted on the bottom of the body 11 to form resistance to the opening of the body 11. By selecting an elastic sleeve 3 with a suitable elastic modulus, the operator can apply a comfortable force to the first arm 13.

[0029] In this embodiment of the present invention, the clamp-like device 1 further comprises a support member 16 and a connector 17 disposed on the top of the body 11. The support member 16 is positioned so as not to overlap with the opening of the body 11 to enhance the rigidity of the body 11. The connector 17 connects the baffle 15 and the support member 16, enhancing the rigidity of the body 11 while also serving as a decorative feature.

[0030] The inner surface of the clamp-like device of the embodiment of the present invention is provided with a heating circuit, so that the experimental container can be heated when the clamp-like device clamps the experimental container. The main body of the clamp-like device forms a cavity surrounding the experimental container. The first arm and the second arm of the clamp-like device use the rotating shaft provided on the main body as a fulcrum, and utilize the lever principle to support or maintain the main body of the clamp-like device, which is easy to load and disassemble and will not affect other operations in the experimental container.

[0031] Example 2

[0032] Figure 5 Schematic diagram of the biological sample preparation device provided in the second embodiment of the present invention. Figure 5 As shown, the biological sample preparation device includes a housing, multiple motors, multiple experimental containers for preparing single-cell suspensions, and multiple heating devices described in Example 1. In this embodiment of the present invention, the structure of the heating device is the same as that of Example 1, including all the features described in Example 1, which will not be repeated here.

[0033] In an embodiment of the present invention, multiple motors are disposed within a housing, their drive shafts connected to the multiple experimental containers, driving the experimental containers to rotate. Simultaneously, multiple heating devices clamp the multiple experimental containers and heat them. The biological sample preparation device also includes a controller that controls the multiple heating devices to maintain the biological samples in the multiple experimental containers at different specific temperatures.

[0034] The inner surface of the clamp-like device of the embodiment of the present invention is provided with a heating circuit, so that the experimental container can be heated when the clamp-like device clamps the experimental container. The main body of the clamp-like device forms a cavity surrounding the experimental container. The first arm and the second arm of the clamp-like device use the rotating shaft provided on the main body as a fulcrum, and utilize the lever principle to support or maintain the main body of the clamp-like device, which is easy to load and disassemble and will not affect other operations in the experimental container.

[0035] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A heating device for an experimental container, characterized in that: include: A clamp-shaped device (1), the inner surface of which matches the outer surface of the experimental container, and is used to clamp the experimental container or release the experimental container; at least one heating circuit (2), the heating circuit (2) being arranged on the inner surface of the clamp-shaped device (1); The clamp-like device (1) comprises a body (11), a rotating shaft (12), a first arm (13) and a second arm (14); the body (11) forms a cavity surrounding the experimental container and has an opening in its vertical direction; the rotating shaft (12) is arranged on the body (11), the first arm (13) extends from the rotating shaft (12) to the top of the body (11), and the second arm (14) extends from the rotating shaft (12) to the opening of the body (11); the first arm (13) and the second arm (14) use the rotating shaft (12) as a fulcrum to maintain or open the body (11).

2. The heating device according to claim 1, characterized in that The first arm (13) extends out of the top of the body (11); the clamp-shaped device (1) further comprises a baffle (15) arranged on the top of the body (11) to limit the first arm (13).

3. The heating device according to claim 2, characterized in that The length of the first arm (13) is greater than the length of the second arm (14).

4. The biological sample preparation device according to claim 2, characterized in that: The clamp-shaped device (1) further comprises a support member (16) and a connecting member (17) arranged on the top of the body (11), wherein the connecting member (17) is used to connect the baffle (15) and the support member (16).

5. The heating device according to claim 1, characterized in that It also includes an elastic sleeve (3) sleeved on the body (11).

6. The heating device according to any one of claims 1 to 5, characterized in that: The at least one heating circuit (2) is a resistance heating circuit.

7. The heating device according to any one of claims 1 to 5, characterized in that: The at least one heating circuit (2) is powered by an external power source.

8. The heating device according to any one of claims 1 to 5, characterized in that: The at least one heating circuit (2) extends to the bottom of the body (11) to be connected to an external power source.

9. A biological sample preparation device, characterized in that: comprising a housing, a plurality of motors, a plurality of experimental containers and a plurality of heating devices according to any one of claims 1 to 8; The multiple motors are arranged in the shell, and the driving shafts of the multiple motors are respectively connected to the multiple experimental containers to drive the multiple experimental containers to rotate. The multiple heating devices respectively clamp the multiple experimental containers for heating.

10. The biological sample preparation device according to claim 9, characterized in that: The system further includes a controller, which controls the plurality of heating devices to heat the plurality of experimental containers.

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