Switchable incubation module, PCR amplification detector

Abstract

The present invention relates to a switchable incubation module, comprising an incubation body for heating a microfluidic chip, wherein the incubation body comprises: a plurality of heating modules for heating a reaction chamber on a microfluidic chip; wherein at least two heating modules have different heating temperatures; a rotary drive structure for driving the microfluidic chip and the heating module to rotate relative to each other; each heating module is provided with the same number of heating zones, and the heating zones on a plurality of heating modules are arranged concentrically with the microfluidic chip; when incubation is started, the heating zones of the heating modules at different heating temperatures of the microfluidic chip rotate in sequence under the drive of the rotary drive structure, so as to switch the heating temperature of the reaction chamber of the microfluidic chip during incubation. The present invention also relates to a PCR amplification detector. Through the switchable incubation module, the temperature required for the amplification reaction of the reaction chamber on the microfluidic chip can be quickly provided.

Description

[Technical field]

[0001] The invention relates to the technical field of fluorescence detection, and in particular to a switchable incubation module and a PCR amplification detector. [Background technology]

[0002] The substance of nucleic acid testing is the nucleic acid of the virus. Nucleic acid testing is to find out whether there is nucleic acid of foreign invading viruses in the patient's respiratory specimens, blood or feces to determine whether the patient is infected with the virus. Therefore, once the nucleic acid test is positive, it can be proved that the virus exists in the patient's body. All organisms contain nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Taking the new coronavirus as an example, it is a virus that only contains RNA. The specific RNA sequence in the virus is a marker that distinguishes the virus from other pathogens. During clinical laboratory testing, if the specific nucleic acid sequence of the new coronavirus can be detected in the patient's sample, it should indicate that the patient may be infected with the new coronavirus.

[0003] The most common method for detecting virus-specific sequences is fluorescent quantitative PCR (polymerase chain reaction). It is a method that uses fluorescent chemicals to measure the total amount of products after each polymerase chain reaction cycle in a DNA amplification reaction, and a method for quantitatively analyzing specific DNA sequences in the sample to be tested through internal or external reference methods. The number of cycles (Ct value) at which the fluorescence reaches a pre-set threshold that can be monitored by a fluorescent quantitative PCR instrument is related to the concentration of viral nucleic acid. The higher the concentration of viral nucleic acid, the smaller the Ct value.

[0004] The traditional nucleic acid detection process has many processing steps and is time-consuming (the traditional PCR amplification step is to heat and cool in a single temperature control chamber, and the heating plate is used to control the temperature change of the PCR reaction chamber.

[0005] It is relatively easy to control the temperature control chip to increase its temperature, but its cooling can only rely on natural convection, which means it takes a long time to achieve temperature conversion. The heating plate is expensive and easy to damage. In addition, PCR has high sensitivity requirements and is easily affected by environmental interference. Traditional nucleic acid testing requires at least 3 negative pressure chambers, equipped with professional personnel and additional equipment such as centrifuges. The construction cost is high, and it is difficult for hospitals below the second level to carry out independently, showing a centralized distribution trend.

[0006] Therefore, it is necessary to improve the prior art to overcome the above defects in the prior art. [Summary of the invention]

[0007] In view of the shortcomings of the prior art, the object of the present invention is to provide a switchable incubation module, including an incubation body for heating a microfluidic chip, wherein the incubation body includes:

[0008] A plurality of heating modules, used for heating the reaction chamber on the microfluidic chip; wherein the heating temperatures on at least two of the heating modules are different;

[0009] A rotation driving structure, used to drive the microfluidic chip and the heating module to rotate relative to each other;

[0010] Each of the heating modules is provided with the same number of heating zones, and the heating zones on a number of the heating modules are arranged concentrically with the microfluidic chip; when the incubation is started, driven by the rotating drive structure, the heating zones of the heating modules with different heating temperatures of the microfluidic chip rotate in sequence to switch the heating temperature of the reaction chamber of the microfluidic chip during incubation.

[0011] Preferably, it also includes a heat-insulating layer, and the heat-insulating layer is used to maintain the temperature inside the incubation body.

[0012] Preferably, the heating zones on a plurality of the heating modules are equidistant from the central axis of the heating module.

[0013] Preferably, the heating zones on different heating modules are at different distances from the central axis of the heating module. After the microfluidic chip and the heating module are driven to rotate relative to each other, the reaction liquid to be heated on the microfluidic chip moves under the action of centrifugal force to contact the heating modules with different heating temperatures.

[0014] Preferably, it also includes a motion mechanism, which is connected to the heating module, and the motion mechanism is controlled to control different heating modules to contact with the reaction chambers on the microfluidic chip in turn.

[0015] Preferably, there are gaps between adjacent heating zones of a plurality of the heating modules, wherein a heating zone on one heating module passes through the gap between heating zones of another heating module to contact the reaction chamber on the microfluidic chip.

[0016] Preferably, at least one of the heating modules includes a first heating body, a first temperature-controlled heating plate and a first thermal insulation fixing block, and the first heating body and the first temperature-controlled heating plate are fixed by the first thermal insulation fixing block; the first heating body extends a plurality of first extension columns along its outer contour toward its central axis, and a first heating column is also provided on the side of the first extension column close to the microfluidic chip, and the first heating column is controlled to move to the reaction chamber on the microfluidic chip to provide a suitable reaction temperature.

[0017] Preferably, the rotation driving structure is used to drive the microfluidic chip to rotate.

[0018] Preferably, the rotary drive mechanism is used to drive a plurality of the heating modules to rotate.

[0019] The present invention also relates to a PCR amplification detector, characterized in that it comprises a reaction module, a switchable incubation module as described above, and a fluorescence detection module which are arranged in sequence from top to bottom.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present invention relates to a switchable incubation module, comprising an incubation body for heating a microfluidic chip, wherein the incubation body comprises: a plurality of heating modules for heating a reaction chamber on a microfluidic chip; wherein at least two heating modules have different heating temperatures; a rotary drive structure for driving the microfluidic chip and the heating module to rotate relative to each other; each heating module is provided with the same number of heating zones, and the heating zones on a plurality of heating modules are arranged concentrically with the microfluidic chip; when incubation is started, the heating zones of the heating modules at different heating temperatures of the microfluidic chip rotate in sequence under the drive of the rotary drive structure, so as to switch the heating temperature of the reaction chamber of the microfluidic chip during incubation. The present invention also relates to a PCR amplification detector. Through the switchable incubation module, the temperature required for the amplification reaction of the reaction chamber on the microfluidic chip can be quickly provided.

[0022] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention in conjunction with the accompanying drawings. The specific implementation of the present invention is given in detail by the following embodiments and their accompanying drawings.

Brief Description of the Drawings

[0023] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0024] Figure 1 It is a schematic diagram of the structure of the incubation module of the present invention;

[0025] Figure 2 It is a schematic diagram of the structure of several heating modules of the present invention;

[0026] Figure 3 It is a structural schematic diagram of the mobile mechanism of the present invention;

[0027] Figure 4 It is a schematic diagram of the structure of different heating bodies of the present invention;

[0028] Figure 5 is a structural schematic diagram of the second heating module of the present invention;

[0029] Figure 6 is a schematic structural diagram of a third heating module of the present invention;

[0030] Figure 7 It is a structural schematic diagram of the first heating module of the present invention;

[0031] Figure 8 It is a structural schematic diagram of the rotary drive structure of the present invention;

[0032] Fig. 9 It is a structural schematic diagram of the fluorescence detection module of the present invention.

[0033] Description of reference numerals:

[0034] 100. PCR amplification detector;

[0035] 101, motion moving mechanism; 1011, bracket; 1012, round shaft; 1013, micro push rod; 1014, vertical plate; 1021, third fixed plate; 1022, second fixed plate; 1023, first fixed plate; 1024, guide rod; 1025, temperature control module fixed plate; 1026, bearing;

[0036] 102, incubation module; 110, first heating module; 111, first extension column; 112, first heating column; 113, first temperature-controlled heating plate; 114, first heat-insulating fixing block; 115, first heating body;

[0037] 120, second heating module; 121, heat-conducting column; 122, second temperature-controlled heating plate; 123, heat-insulating fixed column; 124, second heating body;

[0038] 130, third heating module; 131, second extension column; 132, second heating column; 133, third temperature control heating plate; 134, third heat insulation fixing block; 135, third heating body;

[0039] 103, rotary drive structure; 1031, servo motor; 1032, servo motor vertical plate; 1033, servo motor fixing plate; 1034, chip fixing bracket; 1035, turntable;

[0040] 104, fluorescence detection module; 1041, spectrometer; 1042, light emitter; 1043, imaging detector. [Specific implementation method]

[0041] The present invention is further described in detail below in conjunction with the accompanying drawings, and the aforementioned and other purposes, features, aspects and advantages of the present invention will become more obvious, so that those skilled in the art can implement it with reference to the text of the specification. In the accompanying drawings, for the sake of clarity, the shapes and sizes can be enlarged, and the same reference numerals will be used in all figures to indicate the same or similar parts. In the following description, words such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, etc. are based on the orientation or position relationship shown in the accompanying drawings. In particular, "height" is equivalent to the size from top to bottom, "width" is equivalent to the size from left to right, and "depth" is equivalent to the size from front to back. These relative terms are for the convenience of explanation and are generally not intended to require specific orientation. Terms related to attachment, connection, etc. (for example, "connection" and "attachment") refer to the relationship between these structures directly or indirectly fixed or attached to each other through intermediate structures, and movable or rigid attachment or relationship, unless otherwise explicitly stated.

[0042] Next, the present invention is further described in conjunction with the accompanying drawings and specific embodiments. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment. It should be understood that the terms such as "having", "including" and "comprising" used herein do not specify the existence or addition of one or more other elements or their combinations.

[0043] Embodiment 1

[0044] like Figure 1-9 As shown, the present invention relates to a switchable incubation module 102, including an incubation body for heating a microfluidic chip, the incubation body comprising:

[0045] A plurality of heating modules are used to heat the reaction chamber on the microfluidic chip; wherein the heating temperatures on at least two heating modules are different;

[0046] The rotation driving structure 103 is used to drive the microfluidic chip and the heating module to rotate relative to each other;

[0047] Each heating module is provided with the same number of heating zones (in some embodiments, the heating zones are raised heating zones on the heating modules), and the heating zones on several heating modules are arranged concentrically with the microfluidic chip; when the incubation is turned on, driven by the rotating drive structure, the heating zones of the heating modules of the microfluidic chip with different heating temperatures rotate in sequence to switch the heating temperature of the reaction chamber of the microfluidic chip during incubation. The number of heating modules is related to the number of different temperature values ​​required for the microfluidic chip reaction. When the microfluidic chip is used for PCR amplification reaction, it is generally necessary to set three different temperatures. At this time, the incubation module 102 includes three heating modules, such as Figure 2, 4 As shown, the incubation module 102 includes a plurality of variable temperature zones, each of which includes a heating zone on each heating module. For example, a variable temperature zone includes a heating zone on the first heating module 110 , a heating zone on the second heating module 120 , and a heating zone on the third heating module 130 .

[0048] It should be understood that the incubation body is a common incubation device in the microfluidic chip heating process, generally a sealing structure, etc.

[0049] In some embodiments, the heating module further comprises a heating body, which can be set to any shape, and it is only necessary to ensure that the heating area on the heating body can heat the reaction chamber on the microfluidic chip. It should be understood that the microfluidic chip can be set to any shape. Preferably, the heating body is set to a ring shape, in which case the microfluidic chip can also be set to a disc shape, and the two cooperate to ensure that the heating area can fully contact the reaction chamber.

[0050] In some embodiments, a heat preservation layer is further included to maintain the temperature in the incubation body; preferably, the heat preservation layer is arranged around the periphery of the microfluidic chip to provide a better heat preservation environment for the microfluidic chip.

[0051] In some embodiments, the heating zones on the plurality of heating modules are equidistant from the central axis of the heating modules. Figure 2 As shown, several heating modules are located on the same circumference. At this time, as the microfluidic chip rotates, the heating areas on different heating modules can contact the reaction chambers on the microfluidic chip to provide different heating temperatures.

[0052] In some embodiments, the heating zones on different heating modules are at different distances from the central axis of the heating modules, that is, the different heating modules are not on the same circumference. After the microfluidic chip and the heating module are driven to rotate relative to each other, the reaction liquid to be heated on the microfluidic chip moves under the action of centrifugal force to contact the heating modules with different heating temperatures; as in a specific embodiment, the heating zones on different heating modules are at different positions of the same radius, and the temperatures of the heating zones on different heating modules are set according to the reaction needs; after the microfluidic chip rotates relative to each other, the liquid to be heated in the microfluidic chip moves away from the center of the microfluidic chip under the action of centrifugal force, and successively contacts different heating modules during this movement to achieve heating at different temperatures.

[0053] When driving the microfluidic chip and the heating module to rotate relative to each other, in some embodiments, the rotary drive structure is used to drive the microfluidic chip to rotate. In this case, the rotary drive structure can be a turntable, and the turntable drives the microfluidic chip to rotate. In other embodiments, the rotary drive mechanism is used to drive several heating modules to rotate; in this case, the rotary drive mechanism directly acts on different heating modules.

[0054] In some embodiments, it also includes a motion movement mechanism 101, which is connected to the heating module. The motion movement mechanism is controlled to control different heating modules to contact the reaction chamber on the microfluidic chip in turn. In some embodiments, the motion movement mechanism 101 includes: a fixed plate, a guide rod and a push rod, one end of the guide rod is connected to the fixed plate, and the other end is connected to the heating module; the push rod pushes the fixed plate to move, so as to drive the heating module to move toward the reaction chamber on the microfluidic chip. Specifically, the first heating module 110, the second heating module 120, and the third heating module 130 are respectively fixed to the first fixed plate 1023, the second fixed plate 1022, and the third fixed plate 1021 by the guide rod 1024. The height direction motion control can be achieved in the bearing 1026 through three independently operated push rods such as the micro push rod 1013. Figure 1-4 As shown, it is composed of a bracket 1011, a circular shaft 1012, a micro push rod 1013, and a vertical plate 1014. The two brackets 1011 fixed to the two vertical plates are used to fix the circular shaft 1012. The three micro push rods 1013 are fixed on the circular shaft 1012, and can independently realize control in different directions (three-dimensional directions).

[0055] Furthermore, there are gaps between adjacent heating zones of several heating modules, wherein a heating zone on one heating module passes through the gap between heating zones of another heating module to contact the reaction chamber on the microfluidic chip. That is, if the first heating module 110 is the heating module closest to the microfluidic chip, the heat-conducting column 121 on the second heating module 120 passes through the gap between the first heating columns 112 on the first heating module and the gap between the second heating columns 132 on the third heating module to get close to the microfluidic chip; similarly, the second heating column 132 on the third heating module 130 passes through the gap between the pair of heating columns on the first heating module 110 to get close to the microfluidic chip.

[0056] In some embodiments, when the heating body of the heating module is configured in a ring shape and includes three heating modules, Figure 4-7 As shown, it may specifically include: a first heating module 110, a second heating module 120 and a third heating module 130. The three heating modules may be arranged under the microfluidic chip or arranged above the microfluidic chip at the same time. For example, when the three heating modules are arranged under the microfluidic chip, Figure 4As shown, the first heating module 110, the second heating module 120, and the third heating module 130 are installed one by one from top to bottom below the microfluidic chip; or they are arranged on the upper and lower sides of the microfluidic chip. When the three heating modules are located on the same side of the microfluidic chip, the three heating modules have different diameters, that is, the first heating module 110, the second heating module 120, and the third heating module 130 are mutually nested and have the same center of the circle to ensure that they do not interfere with each other during movement. When the three heating modules are located on different sides of the microfluidic chip, it is ensured that the calibers of the heating modules located on the same side do not interfere with each other. It should be understood that when the heating body is annular, the heating module can heat all the reaction chambers on the microfluidic chip at one time, which is fast and time-saving. When the heating module is not annular and three heating modules are set, the heating module can be set to any shape at this time. In some embodiments, the heating module is different from the shape of the microfluidic chip, but the heating module can fully contact the reaction chamber on the microfluidic chip. In other embodiments, a heating module only corresponds to a partial area on the microfluidic chip. For example, if the microfluidic chip includes N (N can be any positive integer) reaction chambers, that is, corresponding to 16 fluxes, a heating module corresponds to one or more reaction chambers on the microfluidic chip; at this time, it is only necessary to ensure that a number of heating modules are arranged in a staggered manner. The purpose of the staggered arrangement is to ensure that the three heating modules do not interfere with each other when moving along the height direction of the PCR amplification analyzer. When three heating modules are included, three heating modules are arranged in a staggered manner. It should be noted that if the heating module is rotatable, there is no need to limit the staggered distribution of the three heating modules. The target heating module can be rotated to a non-interference position first, and then move along the height direction of the PCR amplification analyzer; at this time, the heating module only heats part of the reaction chamber at one time, but with the relative movement of the microfluidic chip and the heating module, the heating module can complete the heating of all reaction chambers on the microfluidic chip.

[0057] In some embodiments, Figure 7 As shown, the first heating module 110 includes a first heating body 115, which is a temperature-controlled heat-conducting plate. The temperature-controlled heat-conducting plate extends a plurality of first extension columns 111 along its outer contour toward its central axis. The first extension columns 111 are evenly or unevenly distributed along the temperature-controlled heat-conducting plate. A first heating column 112 is also provided on the first extension column 111 near the side where the microfluidic chip is located. The first heating column 112 is recorded as a heating zone on the first heating module 110, that is, the first heating column 112 on the first heating module 110 is controlled to move to the reaction chamber on the microfluidic chip to provide a suitable reaction temperature. The first heating body 115 and the first temperature-controlled heating plate 113 are fixed by a first heat-insulating fixing block 114.

[0058] In some embodiments, Figure 6As shown, the second heating module 120 includes a second heating body 124, which is a temperature-controlled heat-conducting plate. The second heating body 124 is provided with a plurality of heat-conducting columns 121 near the microfluidic chip side. The heat-conducting columns 121 are detachably connected or non-detachably connected to the second heating body 124. The second heating body 124 is fixed to the second temperature-controlled heating plate 122 by a heat-insulating fixing column 123.

[0059] In some embodiments, Figure 5 As shown, the third heating module 130 includes a third heating body 135, which is a temperature-controlled heat-conducting plate. The third heating body 135 extends a plurality of second extension columns 131 along its outer contour to a position away from the central axis of the third heating body 135. The second extension columns 131 are evenly or unevenly distributed along the third heating body 135. A second heating column 132 is also provided on the second extension column 131 near the side where the microfluidic chip is located. The second heating column 132 is recorded as a heating zone on the third heating module 130, that is, the second heating column 132 on the third heating module 130 is controlled to move to the reaction chamber on the microfluidic chip to provide a suitable reaction temperature. The third heating body 135 and the third temperature-controlled heating plate 133 are fixed by a third heat-insulating fixing block 134.

[0060] It should be understood that the first, second, and third do not refer to the importance of the objects, but are only used to distinguish different objects.

[0061] When two or more heating modules are included, the heating module may be configured as one or more of the first heating module 110 , the second heating module 120 , and the third heating module 130 .

[0062] When a heating module is provided with a plurality of extension columns, there is a gap between two adjacent extension columns to accommodate a heating zone on other heating modules. Specifically, when the heating module is the first heating module 110, the temperature-controlled heat-conducting plate extends a plurality of first extension columns 111 along its outer contour toward its central axis, and a gap is formed between two adjacent first extension columns 111 to accommodate the heat-conducting column 121 on the second heating module 120 and the second heating column 132 on the third heating module 130. A variable temperature region is formed between two adjacent first extension columns 111, and the region includes a first heating column 112 corresponding to the first temperature, a heat-conducting column 121 corresponding to the second temperature, and a second heating column 132 corresponding to the third temperature. Furthermore, there is a gap between the heating zones on different heating modules, and the gap is 1cm-5cm to ensure that the temperatures of the heating modules do not affect each other. Specifically, in each variable temperature area, there is a gap of 1cm-5cm between the first heating column 112 and the thermally conductive column 121 , a gap of 1cm-5cm between the thermally conductive column 121 and the second heating column 132 , and a gap of 1cm-5cm between the second heating column 132 and the next first heating column 112 .

[0063] Embodiment 2

[0064] A PCR amplification detector 100 includes a reaction module, a switchable incubation module as in the first embodiment, and a fluorescence detection module which are sequentially arranged from top to bottom.

[0065] The reaction module includes a microfluidic chip, which may be provided with a plurality of reaction chambers for carrying out multiple reactions simultaneously. In some embodiments, the microfluidic chip has 16 PCR reaction chambers corresponding to 16 fluxes.

[0066] like Figure 8 As shown, the rotary drive structure 103 in the incubation module includes a turntable 1035, and the turntable 1035 also includes at least one accommodating area, and the heating column passes through the accommodating area to contact the reaction chamber on the microfluidic chip. In addition, the rotary drive structure 103 also includes a servo motor 1031, a servo motor vertical plate 1032, a servo motor fixing plate 1033, and a chip fixing bracket 1034. The servo motor 1031 is fixed to the temperature control module fixing plate 1025 through the servo motor fixing plate 1033 and the servo motor vertical plate 1032, and the chip fixing bracket 1034 is fixed to the servo motor 1031 to control the turntable 1035 to rotate.

[0067] Fluorescence detection module 104, such as Fig. 9As shown, it is composed of a spectrometer 1041, a light emitter 1042, and an imaging detector 1043. When there are 16 PCR reaction chambers on the microfluidic chip, the micro push rod 1013 is used to push the first heating module 110, the second heating module 120, and the third heating module 130 in turn to contact the PCR reaction chamber on the microfluidic chip, and finally realize the alternating temperature control of the PCR reaction chamber. When the PCR reaction is completed, the light emitter 1042 is turned on, and the light passes through the spectrometer 1041 to reach the PCR reaction chamber, and then is reflected to the spectrometer 1041 and refracted to finally enter the imaging detector 1043 to realize fluorescence detection. Specific implementation application 1: Mycoplasma pneumoniae (MP) nucleic acid detection (PCR fluorescence method)

[0068] The throat swab sample (35 years old, male sample) was added with 1 mL of sterile saline, shaken thoroughly, and the liquid was pipetted into a centrifuge tube and centrifuged at 12,000 RPM for 5 minutes. Through manual operation, 100 μL of precipitation solution and 50 μL of nucleic acid extraction solution were added to the storage tank of the microfluidic chip respectively, and the microfluidic chip was rotated at 2,000 RPM (acceleration of 6,000 RPM / s) for 7 minutes. After the nucleic acid extraction is completed, the microfluidic chip is rotated at 1,500 RPM (acceleration of 3,000 RPM / s) for 10 seconds, and 50 μL of supernatant can be transferred to the PCR reaction chamber. PCR amplification steps: 50°C, 2 minutes, 1 cycle; 95°C, 10 minutes, 1 cycle; 55°C, 45 seconds, 45 cycles. The reporter fluorescence was FAM (excitation wavelength 494 nm, emission wavelength 522 nm), and the measured Ct value was 40, indicating a positive sample.

[0069] Specific implementation application 2: Verify the temperature accuracy of the PCR temperature control module

[0070] Experimental parameters: PCR amplification temperature control is 50℃ (module A), 75℃ (module B), 95℃ (module C), the cycle is 50℃→95℃→75℃, and a total of 40 cycles are tested. Experimental analysis: A multi-channel thermometer is used to measure the temperature of the PCR reaction chamber. Experimental goal: The temperature of the PCR reaction chamber must be maintained at the set value ±0.5℃. Experimental results: When module A (50℃) is switched to module C (95℃), the average temperature of the PCR reaction chamber can reach 95℃±0.25℃ within 92 seconds; when module C (95℃) is switched to module B (75℃), the average temperature of the PCR reaction chamber can reach 75℃±0.31℃ within 85 seconds; when module B (75℃) is switched to module A (50℃), the average temperature of the PCR reaction chamber can reach 50℃±0.41℃ within 105 seconds. The above results show that the present invention can complete the PCR amplification step in a short time.

[0071] Fixed area The various embodiments in this specification are described in a progressive manner, and the same and similar parts between the various embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment.

Claims

1. A switchable incubation module, comprising an incubation body for heating a microfluidic chip, characterized in that: The incubation body comprises: A plurality of heating modules are used to heat the reaction chamber on the microfluidic chip; wherein at least two of the heating modules have different heating temperatures; and the plurality of heating modules are located on the same side of the microfluidic chip; A rotation driving structure, used to drive the microfluidic chip and the heating module to rotate relative to each other; The same number of heating zones are arranged on each of the heating modules, and the heating zones on several of the heating modules are arranged concentrically with the microfluidic chip; the heating zones on the same heating module are at equal distances from the central axis of the heating module; the heating zones on different heating modules are at different distances from the central axis of the heating module; when the incubation is started, driven by the rotary drive structure, the heating zones of the heating modules at different heating temperatures of the microfluidic chip rotate in sequence to switch the heating temperature of the reaction chamber of the microfluidic chip during incubation; the rotary drive structure includes a turntable, which also includes at least one accommodating area, and the heating column passes through the accommodating area to contact the reaction chamber on the microfluidic chip; the rotary drive structure also includes a servo motor, a servo motor vertical plate, a servo motor fixing plate, and a chip fixing bracket; the servo motor is fixed to the temperature control module fixing plate through the servo motor fixing plate and the servo motor vertical plate, and the chip fixing bracket is fixed to the servo motor to control the rotation of the turntable; It also includes a motion mechanism, which is connected to the heating module, and is controlled to control the different heating modules to contact the reaction chambers on the microfluidic chip in turn; the motion mechanism includes: a fixed plate, a guide rod and a micro push rod, one end of the guide rod is connected to the fixed plate, and the other end passes through the temperature control module fixed plate and is connected to the heating module; the micro push rod pushes the fixed plate to move, so as to drive the heating module to move toward the reaction chamber on the microfluidic chip; a plurality of the heating modules are respectively fixed to a plurality of fixed plates through the guide rods, and the height direction motion control is realized through a plurality of independently operated micro push rods; The motion movement mechanism also includes: a bracket, a circular shaft and a vertical plate, wherein the two vertical plates are arranged opposite to each other, and the two brackets are fixed on both sides of the two vertical plates to form a supporting structure; the circular shaft is fixed between the two oppositely arranged brackets, and one end of a plurality of micro push rods is fixed on the circular shaft, and the other end is fixed on the fixed plate to independently realize motion control in different directions.

2. The switchable incubation module according to claim 1, characterized in that Also comprises a heat preservation layer, and the heat preservation layer is used for maintaining the temperature inside the incubation body.

3. The switchable incubation module according to claim 1, characterized in that: There are gaps between adjacent heating zones of a plurality of the heating modules, wherein a heating zone on one heating module passes through the gap between heating zones of another heating module to contact the reaction chamber on the microfluidic chip.

4. The switchable incubation module according to claim 1 or 3, characterized in that: At least one of the heating modules includes a first heating body, a first temperature-controlled heating plate and a first thermal insulation fixing block, wherein the first heating body and the first temperature-controlled heating plate are fixed by the first thermal insulation fixing block; the first heating body extends a plurality of first extension columns along its outer contour in a direction close to its central axis, and a first heating column is also provided on the first extension column on the side where the microfluidic chip is located, and the first heating column is controlled to move to the reaction chamber on the microfluidic chip to provide a suitable reaction temperature.

5. A PCR amplification detector, characterized in that: It comprises a reaction module, a switchable incubation module as claimed in any one of claims 1 to 4, and a fluorescence detection module which are arranged in sequence from top to bottom.

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