A sample analysis method and a sample detection device

Abstract

The application provides a sample analysis method and a sample detection device, which are used for reducing detection time and reducing detection reagent amount. Specifically, a sampling needle is used to suck a sample, the sample including a sample required for primary detection items and a sample required for re-detection items, the re-detection items being determined according to the primary detection items; the sampling needle is used to inject the sample required for the primary detection items into a corresponding first reaction pool according to a preset ratio and inject the sample required for the re-detection items into a second reaction pool, the first reaction pool being used for mixing the sample with reaction reagents required for detection of the primary detection items to prepare a primary detection mixed solution, and the second reaction pool being used for mixing the sample with a dilution solution to form a diluted sample; detection parameters are obtained by detecting the primary detection mixed solution in the first reaction pool; when the detection parameters meet re-detection conditions, a re-detection mixed solution is prepared by mixing the diluted sample and reaction reagents required for re-detection items corresponding to the detection parameters; and re-detection results are obtained by re-detecting the re-detection mixed solution.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medical devices, in particular to a sample analysis method and a sample detection device. BACKGROUND

[0002] In the sample detection process, if an abnormal detection result occurs during the detection process, the sample usually needs to be rechecked to provide further basis for clinical judgment.

[0003] A common rechecking scheme is achieved by a sample retesting method: one is a manual sample injection mode, the user needs to screen the results of the conventional test samples, identify abnormal samples, and find out the corresponding samples for re-detection; in another automatic sample injection mode, the user pre-sets the rechecking rules, and the instrument judges whether the sample needs to be rechecked according to the detection result. When a sample that meets the rechecking condition is found, the sample is returned by the sample injector, re-mixed, sample collected, and rechecking started.

[0004] In these rechecking schemes, the sample needs to be detected twice independently, which results in a long detection time and a large amount of reagent consumption. SUMMARY

[0005] Embodiments of the present application provide a sample analysis method and a sample detection device to reduce the detection time and the amount of reagent.

[0006] In a first aspect, embodiments of the present application provide a sample analysis method and a sample detection device, which specifically include: a sampling needle sucking a sample, the sample sucked by the sampling needle including a sample required for a primary detection item and a sample required for a rechecking item, the rechecking item being determined according to the primary detection item; a sample being divided, the sampling needle dividing the sample required for the primary detection item to a corresponding first reaction pool according to a preset ratio and dividing the sample required for the rechecking item to a second reaction pool, the first reaction pool being used for mixing the sample with a reaction reagent required for the primary detection item to prepare a primary detection mixed solution, and the second reaction pool being used for mixing the sample with a dilution solution to form a diluted sample; detecting the primary detection mixed solution in the first reaction pool to obtain a detection parameter; when the detection parameter meets a rechecking condition, mixing the diluted sample and a reaction reagent required for the rechecking item corresponding to the detection parameter to prepare a rechecking mixed solution; and rechecking the rechecking mixed solution to obtain a rechecking result.

[0007] In a second aspect, embodiments of the present application provide a sample detection device, which specifically includes: a processor, at least one first reaction pool, at least one second reaction pool, a detection device, a sampling needle, and an output device.

[0008] The processor is configured to determine a rechecking item according to a primary detection item.

[0009] The sampling needle is used for sucking the sample, and the sample sucked by the sampling needle includes the sample required by the primary detection item and the sample required by the re-detection item; the sampling needle injects the sample required by the primary detection item into the corresponding first reaction pool according to a preset proportion and injects the sample required by the re-detection item into the second reaction pool,

[0010] The second reaction pool is used for mixing the sample with a dilution liquid to form a diluted sample;

[0011] The first reaction pool is used for mixing the sample with a reaction reagent required by the primary detection item to prepare a primary detection mixed liquid;

[0012] The detection device is used for detecting the primary detection mixed liquid in the first reaction pool to obtain a detection parameter;

[0013] The processor is used for judging whether the detection parameter meets a re-detection condition or not;

[0014] When the detection parameter meets the re-detection condition, the detection device is used for mixing the diluted sample and a reaction reagent required by a re-detection item corresponding to the detection parameter to prepare a re-detection mixed liquid; and the re-detection mixed liquid is re-detected to obtain a re-detection result;

[0015] The output device is used for outputting the detection parameter and the re-detection result.

[0016] In the technical scheme provided by the embodiment of the application, the sample to be detected has been stored in the reaction pool corresponding to the re-detection item, and when the sample detection parameter of the reaction pool corresponding to the primary detection item is abnormal, the sample of the reaction pool corresponding to the re-detection item can be directly used for re-detection without the need of returning the sample in the sample detection pool for re-detection, so that the time for sample returning is saved, and the detection time is reduced. Meanwhile, the sample is collected once, and the second sample extraction is not needed, the sample is not discarded, and the sample is saved. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 FIG. 1 is a schematic structural diagram of a sample detection device in an embodiment of the application;

[0018] Figure 2 FIG. 2 is a schematic diagram of one embodiment of a sample analysis method in an embodiment of the application;

[0019] Figure 3 FIG. 3 is a schematic structural diagram of sampling and injection in a sample detection device in an embodiment of the application;

[0020] Figure 4 FIG. 4 is a schematic flow diagram of a sample analysis method in an embodiment of the application;

[0021] Figure 5 FIG. 5 is another schematic structural diagram of a sample detection device according to an embodiment of the present application. DETAILED DESCRIPTION

[0022] In order to make the purposes, technical solutions and advantages of the present application clearer, the embodiments of the present application are described below with reference to the drawings. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Those skilled in the art can know that the technical solutions provided by the embodiments of the present application are also applicable to similar technical problems as new application scenarios appear.

[0023] The terms "first", "second", and the like in the description and claims of the present application and the above drawings are used to distinguish similar objects, and do not necessarily indicate a specific order or a chronological sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments described herein can be implemented in an order other than that illustrated or described herein. In addition, the terms "comprise" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device comprising a series of steps or modules does not necessarily limit to those steps or modules clearly listed, but can include other steps or modules not clearly listed or inherent to the process, method, product or device. The naming or numbering of the steps appearing in the present application does not mean that the steps in the method flow must be executed in the time / chronological order indicated by the naming or numbering. The flow steps that have been named or numbered can change the execution order according to the technical purpose to be achieved, as long as the same or similar technical effects can be achieved. The division of units appearing in the present application is a logical division, and in actual application, there can be another division manner, for example, a plurality of units can be combined or integrated into another system, or some features can be ignored or not executed, in addition, the coupling or direct coupling or communication connection between the units shown or discussed can be through some interface, the indirect coupling or communication connection between the units can be electrical or other similar forms, which are not limited in the present application. In addition, the units or sub-units described as separate components can or can not be physically separate, can or can not be physical units, or can be distributed into a plurality of circuit units, and some or all of the units can be selected according to actual needs to achieve the purposes of the present application.

[0024] Figure 1An example structure block diagram of a sample detection device 100 is shown in the figure. The sample detection device 100 includes a processor 101, at least one first reaction pool 102, at least one second reaction pool 103, a detection device 104, a sampling needle 105, and an output device 106. The processor 101 is configured to determine a recheck item according to a preliminary check item. The sampling needle 105 is configured to draw a sample, which includes a sample required by the preliminary check item and a sample required by the recheck item. The sampling needle 105 is configured to inject the sample required by the preliminary check item into the corresponding first reaction pool 102 according to a preset ratio, and inject the sample required by the recheck item into the second reaction pool 103. After the sample injection is completed, the second reaction pool 103 is configured to mix the sample with a dilution liquid to obtain a diluted sample. The first reaction pool 102 is configured to mix the sample with a reaction reagent required by the preliminary check item to prepare a preliminary check mixture. The detection device 104 is configured to detect the preliminary check mixture in the first reaction pool 102 to obtain a detection parameter. The processor 101 is configured to determine whether the detection parameter satisfies a recheck condition. When the detection parameter satisfies the recheck condition, the detection device 104 is configured to mix the diluted sample and a reaction reagent required by the recheck item corresponding to the detection parameter to prepare a recheck mixture. The recheck mixture is rechecked to obtain a recheck result. The output device 106 is configured to output the detection parameter and the recheck result.

[0025] In the embodiment, the output device 106 of the sample detection device 100 can be a touch display screen, a liquid crystal display screen, or a liquid crystal display, a television, or a display device independent of the sample detection device 100, or a display screen of a mobile phone or a tablet computer.

[0026] For details, please refer to Figure 2 An embodiment of the sample analysis method includes the following steps.

[0027] 201. The sample detection device draws a sample, which includes a sample required by a preliminary check item and a sample required by a recheck item, wherein the recheck item is determined according to the preliminary check item.

[0028] The sample detection device determines a preliminary check item and a recheck item corresponding to the preliminary check item before detection. Then, the sampling needle in the sample detection device draws a sample, which includes a sample required by the preliminary check item and a sample required by the recheck item.

[0029] In this embodiment, the review item is used to verify whether the abnormal detection result of the primary item is correct, and the review item is determined according to the abnormal detection result of the primary item. In this embodiment, when the sample is a blood sample, the primary item can include red blood cell (RBC) detection, and the corresponding review item can include reticulocyte (RET) detection. Specifically, in the detection process of the blood sample, clinical judgment of anemia is mainly made by the detection result of RBC. If the RBC parameter is abnormal, the detection result of RET is added to provide further basis for clinical judgment. At the same time, the number of reaction pools for review in the sample detection equipment is limited, and the sample detection equipment can only select part of the review items corresponding to the primary items. In an exemplary scheme, the sample detection equipment can select the review items corresponding to the primary items whose review probability is greater than a preset threshold in the primary items or select the review items corresponding to the primary items whose importance level is higher. For example, in a detection including multiple primary items, if the preset threshold is 60%, and the detection result of item A in the primary items has a review probability greater than 60%, the review item is determined according to the detection result of the item A; or in a detection including multiple primary items, if the detection result of the primary item A is very important, the review item can be determined according to the detection result of the primary item A. In actual application, when the number of reaction pools corresponding to the review items is limited and less than the number of review items corresponding to the primary items, the sample required by the review items can be taken from the sample demand of the review items from large to small. For example, the sample detection equipment only uses 2 reaction pools as the reaction pools corresponding to the review items, but the reaction corresponding to the primary items has 4, and the review items determined according to the detection result of the primary items also have 4, and the required samples are 0.1 ml, 0.2 ml, 0.1 ml and 0.15 ml respectively; then the sample detection equipment injects 0.2 ml and 0.15 ml of sample into the reaction pools corresponding to the review items respectively, and the sample taken by the sampling needle needs to include the samples corresponding to the 4 primary items and the 0.35 ml sample.

[0030] It can be understood that the initial test item and the retest item can be input by the user through the input device, or the sample detection device can determine the retest item corresponding to the initial test item according to the mapping relationship table of the initial test item and the retest item after obtaining the initial test item input by the user. For example, the input device of the sample detection device can be a touch screen, and the touch screen can display an input box, and then the user inputs the initial test item and the retest item through the input box. Alternatively, the touch screen displays options of each test item, and the initial test item and the retest item are obtained by clicking the options on the touch screen. In this embodiment, the initial test item and the retest item can also be input in other ways, such as setting a button to input the initial test item and the retest item through the button.

[0031] 202. The sample detection device divides and injects the sample required by the initial test item into the corresponding first reaction pool and divides and injects the sample required by the retest item into the second reaction pool according to a preset ratio, wherein the first reaction pool is used to mix the sample with a reaction reagent required for initial test item detection to prepare an initial test mixture, and the second reaction pool is used to mix the sample with a diluent to form a diluted sample.

[0032] After the sampling needle of the sample detection device sucks the sample, the sample detection device divides and injects the sample according to a preset ratio. That is, the sample in the sampling needle is injected into the first reaction pool corresponding to the initial test item according to the sample required by the initial test item, and the sample in the sampling needle is injected into the second reaction pool corresponding to the retest item according to the sample required by the retest item. After the sample is divided and injected, the sample in the first reaction pool is mixed with the reaction reagent required for initial test item detection to prepare an initial test mixture, and the sample in the second reaction pool is mixed with a diluent to form a diluted sample.

[0033] In this embodiment, the first reaction pool is the reaction pool corresponding to the initial test item, and the second reaction pool is the reaction pool corresponding to the retest item. The number of the first reaction pool and the second reaction pool is at least one.

[0034] In this embodiment, when the sample is a blood sample, the initial test item includes RBC detection, and the retest item includes RET, the sample detection device can first divide and inject the blood sample required for RET detection into the second reaction pool according to the preset ratio when dividing and injecting the sample; and then divide and inject the blood sample required for RBC detection into the first reaction pool according to the preset ratio. That is, when dividing and injecting the blood sample, the sample for RET detection is injected first, and then the sample for RBC detection is injected. Since the platelet (PLT) parameter of reticulocyte detection is easy to adhere to the needle wall of the sampling needle, in order to reduce the adhesion, the blood sample close to the needle tip is allocated to the reticulocyte reaction pool as much as possible.

[0035] 203、the sample detection device detects the preliminary detection mixture in the first reaction pool to obtain a detection parameter.

[0036] the sample detection device detects the preliminary detection mixture in the first reaction pool according to a detection procedure to obtain a detection parameter.

[0037] It can be understood that in this embodiment, the detection parameter can be a final detection result of the preliminary detection item, or can be a real-time detection value of real-time change in the preliminary detection item. For example, in a blood sample detection process, the detection parameter can be a final detection result, which includes red blood cell count, white blood cell count, platelet count, etc. If the detection parameter is a real-time detection value, the real-time detection value can be the red blood cell count, white blood cell count, platelet count, etc. at the current time in the detection process.

[0038] 204、when the detection parameter meets the re-detection condition, the sample detection device mixes the diluted sample and a reaction reagent required for a re-detection item corresponding to the detection parameter to prepare a re-detection mixture.

[0039] In this embodiment, the detection parameter meeting the re-detection condition can be as follows: when the final detection result of the preliminary detection item exceeds a normal range, it is determined that the final detection result meets the re-detection condition, and the final detection result is the detection parameter; or, when a real-time detection value in the preliminary detection item test process exceeds the normal range, it is determined that the real-time detection value meets the re-detection condition, and the real-time detection value is the detection parameter. In an exemplary scheme, in a blood sample detection process, the detection parameter can be a final detection result, which includes red blood cell count, white blood cell count, platelet count, etc.; when the red blood cell count in the final detection result is displayed to exceed the normal range, the sample detection device determines that the red blood cell detection needs to be re-detected. If the detection parameter is a real-time detection value, the real-time detection value can be the red blood cell count, white blood cell count, platelet count, etc. at the current time in the detection process; if it is found that the red blood cell count has exceeded the normal range in the detection process, or the red blood cell count in the final detection result is predicted to exceed the normal range according to the red blood cell count at the current time, the sample detection device determines that the red blood cell detection needs to be re-detected.

[0040] When the sample detection device determines that re-detection is required, the sample detection device selects a reaction pool corresponding to the re-detection item from the second reaction pool, and mixes the diluted sample with a reaction reagent required for the re-detection item to prepare a re-detection mixture.

[0041] It can be understood that in the embodiment, the sample detection device can directly prepare the recheck mixed solution in the second reaction pool, or can suck the diluted sample from the second reaction pool to a detection reaction pool, and then prepare the recheck mixed solution in the detection reaction pool. The specific process is not limited here.

[0042] In the embodiment, if the detection parameters of the recheck item are all within the normal range, the sample detection device empties the diluted sample in the second reaction pool and cleans the second reaction pool.

[0043] 205. The sample detection device rechecks the recheck mixed solution to obtain a recheck result.

[0044] After the recheck mixed solution is prepared, the sample detection device detects the recheck mixed solution according to a normal detection process, so as to obtain a recheck result.

[0045] In the embodiment, after the recheck result is obtained, the sample detection result can comprehensively judge the recheck result and the detection parameters of the recheck item, so as to obtain a final detection result of the sample.

[0046] In an exemplary scheme of the embodiment, the first reaction pool and the second reaction pool of the sample detection device can be as shown in Figure 3 The sample detection device includes a sampling needle, a RET reaction pool, and a reaction pool 1 and a reaction pool 2. The RET reaction pool is used for RET recheck, the reaction pool 1 and the reaction pool 2 are used for regular detection, and the sampling needle is used for sucking a sample. The blood sample required for the RET detection and the blood sample required for the regular detection of the reaction pool 1 and the reaction pool 2. The specific operation process can be as shown in Figure 4As shown, after starting sample measurement, sample collection is performed. Specifically, sample collection is mainly performed by the sampling needle sucking blood samples required for the RET detection and blood samples required for routine detection of the reaction pool 1 and the reaction pool 2; then reaction pool blood distribution is performed, specifically, the sampling needle distributes the blood samples in the sampling needle according to the blood samples required for the detection items corresponding to each reaction pool (for example, the RET reaction pool injects the blood samples required for the RET detection, the reaction pool 1 injects the blood samples required for the detection items corresponding to the reaction pool 1, and the reaction pool 2 injects the blood samples required for the detection items corresponding to the reaction pool 2); then the reaction pool 1 and the reaction pool 2 normally perform routine detection, while the RET reaction pool is in a waiting state, at which time the sample in the RET reaction pool is mixed with a diluent to prepare a diluted sample; during the test of the reaction pool 1 and the reaction pool 2, the detection parameters of the reaction pool 1 and the reaction pool 2 are judged to determine whether the RET recheck needs to be started; if so, the detection of the reaction pool 1 and the reaction pool 2 ends, and the ready state is restored, at the same time, the reagents required for the RET detection are added to the diluted sample in the RET reaction pool to prepare a RET recheck mixed solution, then the RET recheck mixed solution is detected to obtain a recheck result, and finally the detection ends and the ready state is restored; if not, the detection of the reaction pool 1 and the reaction pool 2 ends, and the ready state is restored, while the RET reaction pool is emptied of the diluted sample and the ready state is restored.

[0047] The sample analysis method in the embodiments of the present application is described above, and the sample detection device in the embodiments of the present application is described below. For details, please refer to Figure 5 As shown, the sample detection device 500 in the embodiments of the present application includes an input device 501, a processor 502 and an output device 503. The sample detection device 500 can be a sample detection device in the above method embodiments, or one or more chips in the sample detection device. The sample detection device 500 can be used to perform part or all of the functions of the sample detection device in the above method embodiments.

[0048] For example, the input device 501 can be used to perform part of the steps in step 201 in the above method embodiments. For example, the input device 501 acquires the primary detection items and the recheck items corresponding to the primary detection items.

[0049] The processor 502 can be configured to perform steps 201-205 in the above method embodiments. For example, the processor 502 controls the sampling needle to suck a sample, which includes a sample required for an initial detection item and a sample required for a re-detection item; controls the sampling needle to inject the sample required for the initial detection item into a corresponding first reaction pool according to a preset ratio, and inject the sample required for the re-detection item into a second reaction pool, wherein the first reaction pool is used to mix the sample with a reaction reagent required for initial detection to prepare an initial detection mixture, and the second reaction pool is used to mix the sample with a dilution liquid to form a diluted sample; detects the initial detection mixture in the first reaction pool to obtain a detection parameter; when the detection parameter meets a re-detection condition, mixes the diluted sample and a re-detection item required reaction reagent corresponding to the detection parameter to prepare a re-detection mixture; and detects the re-detection mixture to obtain a re-detection result.

[0050] The output device 503 can be configured to output the detection parameter and the re-detection result.

[0051] Optionally, the sample detection device 500 further includes a storage module, which is coupled to the processor, so that the processor can execute computer execution instructions stored in the storage module to realize the functions of the sample detection device in the above method embodiments. In an example, the optional storage module included in the sample detection device 500 can be a storage unit in a chip, such as a register, a cache, etc. The storage module can also be a storage unit outside the chip, such as a read-only memory (ROM) or other types of static storage devices that can store static information and instructions, a random access memory (RAM), etc.

[0052] It should be understood that the above Figure 5 The processes performed between the modules of the sample detection device in the corresponding embodiments are similar to the processes performed by the sample detection device in the above method embodiments, and thus will not be described in detail here. Figures 2 to 4 The processes performed between the modules of the sample detection device in the corresponding embodiments are similar to the processes performed by the sample detection device in the above method embodiments, and thus will not be described in detail here.

[0053] The steps of a method or algorithm described in connection with the present disclosure can be embodied directly in hardware, in a software module executed by a processor, or in a combination of the two. A software module can reside in RAM memory, flash memory, ROM memory, EPROM memory, EEPROM memory, registers, hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to the processor such that the processor can read information from, and write information to, the storage medium. In the alternative, the storage medium can be integral to the processor. The processor and the storage medium can reside in an ASIC. Alternatively, the processor and the storage medium can be located in a remote terminal. The processor and the storage medium can also be located in a client and a remote server, respectively, and communicate over a network. The processor can include one or more processors from any processor family.

[0054] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working process of the system, device and unit described above can refer to the corresponding process in the foregoing method embodiments, which will not be repeated here.

[0055] In several embodiments provided in the present application, it should be understood that the disclosed system, device and method can be implemented in other ways. For example, the device embodiments described above are merely illustrative, for example, the division of the units is only a logical function division, and actual implementation can have another division manner, for example, a plurality of units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the coupling or direct coupling or communication connection between the units shown or discussed can be indirect coupling or communication connection through some interfaces, devices or units, which can be electrical, mechanical or other forms.

[0056] The units described as separate components can or can not be physically separate, and the components shown as units can or can not be physical units, that is, they can be located in one place, or can be distributed on a plurality of network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the embodiment scheme.

[0057] In addition, each functional unit in each embodiment of the present application can be integrated in one processing unit, or each unit can exist physically, or two or more units can be integrated in one unit. The integrated unit can be realized in the form of hardware or in the form of a software functional unit.

[0058] The integrated unit, if implemented in the form of a software function unit and sold or used as an independent product, can be stored in a computer readable storage medium. Based on such understanding, the technical solutions of the present application or the entire or part of the technical solutions that essentially contribute to the prior art can be embodied in the form of a software product. The computer software product is stored in a storage medium and includes a plurality of instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the method described in the various embodiments of the present application. The aforementioned storage medium includes: a U disk, a mobile hard disk, a read-only memory (ROM, Read-Only Memory), a random access memory (RAM, Random Access Memory), a magnetic disk or an optical disk, and various media that can store program codes.

[0059] The above-described embodiments are only used to illustrate the technical solutions of the present application, rather than limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacements for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method of sample analysis, characterized by, The method comprises the following steps: a sampling needle sucks a sample, the sample sucked by the sampling needle comprises a sample required by a primary detection item and a sample required by a re-detection item, and the re-detection item is determined according to the primary detection item; the sampling needle divides and injects the sample required by the primary detection item into a corresponding first reaction pool and divides and injects the sample required by the re-detection item into a second reaction pool according to a preset proportion, the first reaction pool is used for mixing the sample with a reaction reagent required by the primary detection item to prepare a primary detection mixed solution, and the second reaction pool is used for mixing the sample with a dilution solution to form a diluted sample; a detection parameter is obtained by detecting the primary detection mixed solution in the first reaction pool; when the detection parameter meets a re-detection condition, a re-detection mixed solution is prepared by mixing the diluted sample and a reaction reagent required by a re-detection item corresponding to the detection parameter, and a re-detection result is obtained by re-detecting the re-detection mixed solution; if the detection parameter does not meet the re-detection condition, the reaction reagent required by the re-detection item corresponding to the detection parameter is not added to the diluted sample, and the diluted sample in the second reaction pool is emptied.

2. The method of claim 1, wherein, The re-detection item is used for verifying whether an abnormal detection result of the primary detection item is correct, and the re-detection item is determined according to the abnormal detection result of the primary detection item.

3. The method of claim 1, wherein, The method comprises the following steps: when a final detection result of the primary detection item exceeds a normal range, it is determined that the final detection result meets a re-detection condition, and the final detection result is the detection parameter; or, when a real-time detection value in a test process of the primary detection item exceeds a normal range, it is determined that the real-time detection value meets a re-detection condition, and the real-time detection value is the detection parameter.

4. The method according to any one of claims 1 to 3, characterized in that, The sample is a blood sample, the primary detection item comprises red blood cell (RBC) detection, and the re-detection item comprises reticulocyte (RET) detection.

5. The method of claim 4, wherein, The sampling needle divides and injects the sample required by the primary detection item into the corresponding first reaction pool and divides and injects the sample required by the re-detection item into the second reaction pool according to the preset proportion, which comprises the following steps: the sampling needle divides and injects the blood sample required by the RET detection into the second reaction pool according to the preset proportion; the sampling needle divides and injects the blood sample required by the RBC detection into the first reaction pool according to the preset proportion.

6. The method according to any one of claims 1 to 3, characterized in that, The mixing of the diluted sample and the reaction reagent required by the corresponding re-detection item to prepare the re-detection mixed solution comprises the following steps: the diluted sample and the reaction reagent required by the corresponding re-detection item are mixed in the second reaction pool to prepare the re-detection mixed solution; or, the diluted sample is sucked from the second reaction pool to a reaction pool corresponding to a re-detection item required to be detected; the diluted sample and the reaction reagent required by the re-detection item are mixed in the reaction pool corresponding to the re-detection item required to be detected to prepare the re-detection mixed solution.

7. The method according to any one of claims 1 to 3, characterized in that, The method further comprises the following steps: a final detection result of the sample is determined according to the detection parameter and the re-detection result.

8. The method according to any one of claims 1 to 3, characterized in that, The first reaction pool is a reaction pool corresponding to the primary detection item, and the second reaction pool is a reaction pool corresponding to the re-detection item.

9. The method of claim 8, wherein, The number of the second reaction pools is at least one, and the number of the first reaction pools is at least one.

10. The method according to any one of claims 1 to 3, characterized in that, If the detection parameter does not meet the recheck condition, the dilution sample in the second reaction pool is emptied, and the second reaction pool is cleaned.

11. A sample testing device, characterized by, Comprise: A processor, at least one first reaction pool, at least one second reaction pool, a detection device, a sampling needle, and an output device; The processor is configured to determine a recheck item according to a preliminary check item; The sampling needle is configured to suck a sample, wherein the sample sucked by the sampling needle comprises a sample required by the preliminary check item and a sample required by the recheck item; and the sampling needle is configured to inject the sample required by the preliminary check item into a corresponding first reaction pool and inject the sample required by the recheck item into a second reaction pool according to a preset ratio, The second reaction pool is configured to mix the sample with a dilution liquid to form a dilution sample; The first reaction pool is configured to mix the sample with a reaction reagent required by the preliminary check item to prepare a preliminary check mixed liquid; The detection device is configured to detect the preliminary check mixed liquid in the first reaction pool to obtain a detection parameter; The processor is configured to determine whether the detection parameter meets a recheck condition; When the detection parameter meets the recheck condition, the detection device is configured to mix the dilution sample and a reaction reagent required by a recheck item corresponding to the detection parameter to prepare a recheck mixed liquid; and the detection device is configured to recheck the recheck mixed liquid to obtain a recheck result; if the detection parameter does not meet the recheck condition, the detection device is configured not to add the reaction reagent required by the recheck item corresponding to the detection parameter to the dilution sample, and to empty the dilution sample in the second reaction pool; The output device is configured to output the detection parameter and the recheck result.

12. The apparatus of claim 11, wherein, The processor is configured to determine that a final detection result of the preliminary check item meets a recheck condition when the final detection result exceeds a normal range, wherein the final detection result is the detection parameter; Or, The processor is configured to determine that a real-time detection value meets a recheck condition when the real-time detection value exceeds a normal range during a test process of the preliminary check item, wherein the real-time detection value is the detection parameter.

13. The apparatus of any one of claims 11-12, wherein, The sample is a blood sample, the preliminary check item comprises red blood cell (RBC) detection, and the recheck item comprises reticulocyte (RET) detection.

14. The apparatus of any one of claims 11-12, wherein, The detection device is specifically configured to mix the dilution sample and a reaction reagent required by a corresponding recheck item in the second reaction pool to prepare the recheck mixed liquid; Or, The detection device is specifically configured to suck the dilution sample from the second reaction pool to a reaction pool corresponding to a recheck item required by the detection parameter; The detection device is specifically configured to mix the dilution sample and a reaction reagent required by the recheck item in the reaction pool corresponding to the recheck item to prepare a recheck mixed liquid.

15. The apparatus of any one of claims 11-12, wherein, The number of the second reaction pools is at least one, and the number of the first reaction pools is at least one.

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