A brain drug administration observation cranial window and experimental animal fixed observation device

Abstract

The invention discloses a brain drug administration observation cranial window and an experimental animal fixed observation device. The brain drug administration observation cranial window comprises a body and a light-transmitting plate. The body is provided with a protruding portion. The end surface of the protruding portion is provided with a light-transmitting hole and a first perforation. The peripheral surface of the protruding portion is provided with a second perforation. The first perforation and the second perforation are used to cooperate with a drug administration tube of a drug administration pump. The light-transmitting plate is matched with the protruding portion to cover the light-transmitting hole. The brain drug administration observation cranial window can not only perform precise local drug administration without directly exposing tissues, but also perform microscopic imaging at the same time, and can also realize multiple drug administration and repeated observation at any time. It is simple to operate and convenient to use.

Description

Technical Field

[0001] The invention relates to the technical field of animal experimental devices, in particular to a cranial window for observing brain drug administration and an experimental animal fixing and observing device. Background Art

[0002] The brain is the most complex organ in the human body, and the research methods have been constantly updated and improved. At present, dual / multi-photon microscopes use near-infrared ultrafast lasers as excitation light sources. Due to their low cytotoxicity and deeper penetration into samples, they are particularly suitable for real-time imaging of living brain neurons, glial cells, cerebral blood flow, and tissue oxidative metabolism in the field of neuroscience. So far, dual / multi-photon imaging has become an important and indispensable experimental method in brain science research, and more experimental devices supporting dual / multi-photon microscope live imaging are urgently needed.

[0003] In the process of dual / multi-photon in vivo brain imaging, the subject's brain must first be fixed with a head frame to ensure the stability of the animal brain observation plane image, in order to obtain long-term, high-quality imaging images. In the imaging experiment of acute stimulation and drug administration in vivo, in addition to installing a fixed head frame, an acute surgical craniotomy window is usually used to drip drugs or stimulants on the surface of brain tissue for dual / multi-photon imaging; or after installing a fixed head frame, the drug is administered and a glass head window is sealed on the skull surface for observation.

[0004] At present, the acute window opening method during observation has problems such as easy activation of exposed cells, uncontrollable drug concentration, and corrosion to the objective lens; the method of installing a glass head window has problems such as repeated drug administration and observation at the same time of drug administration, which leads to complicated surgical operations. Summary of the invention

[0005] The first purpose of the present invention is to provide a cranial window for observing brain drug administration, which can not only perform precise local drug administration without directly exposing tissues but also perform microscopic imaging at the same time. It can also realize multiple drug administration and repeated observation at any time, and is simple to operate and easy to use.

[0006] The second purpose of the present invention is to provide a device for fixing and observing experimental animals, which can fix the experimental animals while administering drugs and observing them, and is convenient to operate and use.

[0007] In order to achieve the above technical effects, the technical solution of the present invention is as follows:

[0008] The present invention discloses a cranial window for observing brain drug administration, comprising: a main body, a protruding portion is provided on the main body, a light-transmitting hole and a first perforation are provided on the end surface of the protruding portion, a second perforation is provided on the peripheral surface of the protruding portion, the first perforation and the second perforation are used to cooperate with a drug administration tube of a drug administration pump; and a light-transmitting plate, the light-transmitting plate is matched on the protruding portion to cover the light-transmitting hole.

[0009] In some embodiments, a matching groove arranged around the light-transmitting hole is provided on the end surface of the protruding portion, and the light-transmitting plate is fitted in the matching groove.

[0010] In some specific embodiments, the light-transmitting plate is interference-fitted with the matching groove, or the light-transmitting plate is bonded in the matching groove.

[0011] In some embodiments, the main body is further provided with a fixing hole, and a fixing piece for fixing the head of the experimental subject is passed through the fixing hole.

[0012] In some specific embodiments, there are multiple fixing holes, and the multiple fixing holes are evenly spaced around the protrusion.

[0013] In some embodiments, the light-transmitting plate includes a glass plate or a transparent resin plate.

[0014] The present invention also discloses an experimental animal fixing and observation device, comprising the above-mentioned brain drug administration observation cranial window, a fixing frame and a drug administration pump, wherein the drug administration pump is connected to the drug administration tube, and the brain drug administration observation cranial window is installed on the fixing frame.

[0015] In some embodiments, the experimental animal fixing and observation device further includes an adjustment mechanism, wherein the adjustment mechanism is connected to the main body and is used to adjust the height of the main body.

[0016] In some specific embodiments, the adjustment mechanism includes: an adjustment block, on which a threaded hole is provided; an adjustment knob, which has a stud that cooperates with the threaded hole, and one end of the stud cooperates with the body.

[0017] In some specific embodiments, there are two adjusting mechanisms, and the two adjusting mechanism structures are respectively connected to two ends of the body.

[0018] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic diagram of the structure of a cranial window for observing brain drug administration according to an embodiment of the present invention;

[0020] Figure 2 2 is a schematic structural diagram of a cranial window for observing drug delivery to the brain without a light-transmitting plate removed;

[0021] Figure 3 2 is a schematic diagram of the structure of a fixed observation device for experimental animals according to an embodiment of the present invention;

[0022] Figure 4 It is a structural schematic diagram of the adjustment mechanism of the experimental animal fixing and observation device according to an embodiment of the present invention.

[0023] Reference numerals:

[0024] 1. body; 11. protruding portion; 111. light-transmitting hole; 112. first through hole; 113. second through hole; 114. matching groove; 12. fixing hole;

[0025] 2. Translucent board;

[0026] 3. Fixed frame;

[0027] 4. Adjustment mechanism; 41. Adjustment block; 42. Adjustment knob;

[0028] 5. Dosing pump; 51. Dosing tube. DETAILED DESCRIPTION

[0029] In order to make the technical problem solved by the present invention, the technical solution adopted and the technical effect achieved more clearly, the technical solution of the present invention is further explained below with reference to the accompanying drawings and through specific implementation methods.

[0030] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0031] In addition, features defined as "first" or "second" may explicitly or implicitly include one or more of the features, and are used to distinguish and describe features, without distinction of order or importance. In the description of the present invention, unless otherwise specified, "plurality" means two or more.

[0032] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] Reference below Figure 1-Figure 2 The specific structure of the cranial window for observing brain drug administration according to an embodiment of the present invention is described.

[0034] The present invention discloses a cranial window for observing brain drug administration, such as Figure 1-Figure 2 As shown, the brain drug administration observation cranial window of this embodiment includes a main body 1 and a light-transmitting plate 2. A protrusion 11 is provided on the main body 1. A light-transmitting hole 111 and a first perforation 112 are provided on the end face of the protrusion 11. A second perforation 113 is provided on the circumferential surface of the protrusion 11. The first perforation 112 and the second perforation 113 are used to cooperate with the drug administration tube 51 of the drug administration pump 5. The light-transmitting plate 2 is matched on the protrusion 11 to cover the light-transmitting hole 111.

[0035] It is understandable that, in the actual experimental process, after the light-transmitting plate 2 is mounted on the body 1, the protruding portion 11 of the body 1 is inserted into the head of the experimental animal, and the light-transmitting plate 2 is inserted into the experimental opening of the head of the experimental subject and the experimental opening is closed to prevent the brain tissue of the animal from contacting with the external air. Then the dosing tube 51 of the dosing pump 5 is passed through the second perforation 113 and the first perforation 112 in sequence, so that the end of the dosing tube 51 can reach the experimental opening. After the experimental preparation work is completed, the microscope used for observation is adjusted so that the lens barrel of the microscope corresponds to the light-transmitting hole 111 and the experiment can be started. During the experiment, multiple dosing can be achieved by adjusting the dosing pump 5, and there is no need to disassemble the body 1 during the dosing process, which avoids the phenomenon that the experimental opening of the experimental animal is exposed to the air.

[0036] Therefore, compared with the existing simple glass head window, the brain drug administration observation cranial window of this embodiment can repeatedly administer drugs for a long time, and can achieve drug administration while imaging observation, and can also accurately control the drug dosage in conjunction with the drug administration pump 5, while solving the problem of complicated operation of separating the head frame and the head window. Compared with the method of directly administering drugs through the cranial window, it can solve the problems of easy activation of exposed tissue cells, uncontrollable concentration of drug solution, and corrosion of the objective lens in the experiment.

[0037] In some embodiments, a matching groove 114 is provided on the end surface of the protrusion 11 around the light-transmitting hole 111, and the light-transmitting plate 2 is matched in the matching groove 114. It can be understood that the matching of the light-transmitting plate 2 in the matching groove 114 can improve the installation stability of the light-transmitting plate 2 on the one hand, and prevent the light-transmitting plate 2 from falling off, and on the other hand, it can prevent the light-transmitting plate 2 from protruding from the protrusion 11, thereby preventing the edge of the light-transmitting plate 2 from scratching the operator or the experimental animal tissue.

[0038] In some specific embodiments, the light-transmitting plate 2 is interference-fitted with the matching groove 114, or the light-transmitting plate 2 is bonded in the matching groove 114. Thus, the connection stability between the light-transmitting plate 2 and the body 1 can be further improved.

[0039] In some embodiments, the body 1 is further provided with a fixing hole 12, and a fixing piece for fixing the head of the experimental subject is inserted into the fixing hole 12. It can be understood that the body 1 is further provided with a fixing hole 12, and a fixing piece for fixing the head of the experimental subject can be inserted into the fixing hole 12, that is, the brain drug administration observation cranial window of this embodiment can be directly used as a head frame for fixing the experimental subject, which facilitates the fixation of the brain drug administration observation cranial window, simplifies the experimental device, and facilitates the user to conduct experiments.

[0040] In some specific embodiments, there are multiple fixing holes 12, and the multiple fixing holes 12 are evenly spaced around the protrusion 11. Therefore, multiple fixing parts can be used to fix the head of the experimental subject, which improves the connection stability between the brain drug administration observation cranial window of this embodiment and the experimental subject, ensuring that the experiment can be carried out normally.

[0041] Of course, in other embodiments of the present invention, the number, arrangement and shape of the fixing holes 12 can be selected according to actual needs, and the number, arrangement and shape of the fixing holes 12 are not limited herein.

[0042] In some embodiments, the light-transmitting plate 2 includes a glass plate or a transparent resin plate. It is understandable that the use of a glass plate or a transparent resin plate reduces the quality and manufacturing cost of the brain drug administration observation cranial window and facilitates use. In other embodiments of the present invention, the light-transmitting plate 2 can also be made of other transparent materials.

[0043] Example:

[0044] Reference below Figure 1-Figure 2 The specific structure of the cranial window for observing brain drug administration according to a specific embodiment of the present invention is described.

[0045] like Figure 1-Figure 2As shown, the brain medication observation cranial window of this embodiment includes a main body 1 and a light-transmitting plate 2. A protruding portion 11 is provided on the main body 1. A light-transmitting hole 111, a first perforation 112 and a matching groove 114 arranged around the light-transmitting hole 111 are arranged on the end surface of the protruding portion 11. A second perforation 113 is arranged on the peripheral surface of the protruding portion 11. The light-transmitting plate 2 is bonded in the matching groove 114 by light-curing glue. The first perforation 112 and the second perforation 113 are used to cooperate with the medication tube 51 of the medication pump 5. The light-transmitting plate 2 is matched on the protruding portion 11 to cover the light-transmitting hole 111. The main body 1 is also provided with four fixing holes 12 arranged around the protruding portion 11. The fixing holes 12 are penetrated by fixing holes 12 for fixing the main body 1 to the skull of the experimental subject by means of skull pins and dental cement.

[0046] The length of the main body 1 is 40 mm and the width is 6 mm. The fixing hole 12, the first through hole 112 and the second through hole 113 are all circular holes with a diameter equal to 0.5 mm. The outer circle diameter of the protrusion 11 is 4.4 mm, and the diameter of the light-transmitting hole 111 is 2 mm; the depth of the protrusion 11 is 0.8 mm; the diameter of the matching groove 114 is 3 mm and the depth is 0.1 mm.

[0047] The advantages of the brain drug administration observation cranial window of this embodiment are as follows:

[0048] First: It can perform dual / multi-photon imaging while administering drugs locally without exposing tissues, ensuring the accuracy of the experiment

[0049] Second: Precisely control the dosage and concentration of drug delivery while imaging, easy to operate and use;

[0050] Third: It is convenient for long-term medication and long-term repeated observation;

[0051] Fourth: The head frame and the cranial window for drug administration and observation are integrated to simplify the surgical operation.

[0052] Reference below Figure 3-Figure 4 The specific structure of the cranial window for observing brain drug delivery according to an embodiment of the present invention is described.

[0053] The present invention also discloses an experimental animal fixing and observation device, comprising the above-mentioned brain drug administration observation cranial window, a fixing frame 3 and a drug administration pump 5, wherein the drug administration pump 5 is connected to a drug administration tube 51, and the brain drug administration observation cranial window is installed on the fixing frame 3.

[0054] It is understandable that, in the actual experiment, the experimental subject is placed on the fixing frame 3, and after the light-transmitting plate 2 is installed on the main body 1, the main body 1 is installed on the head of the experimental animal, and the light-transmitting plate 2 is arranged opposite to the experimental opening of the head of the experimental subject, and the experimental opening is closed to prevent the brain tissue of the animal from contacting with the external air. Then, the dosing tube 51 of the dosing pump 5 is passed through the second perforation 113 and the first perforation 112 in sequence, so that the end of the dosing tube 51 can reach the experimental opening. During the experiment, multiple dosing can be achieved by adjusting the dosing pump 5, and there is no need to disassemble the main body 1 during the dosing process, thereby preventing the experimental opening of the experimental animal from being exposed to the air.

[0055] Therefore, due to the brain drug administration observation cranial window mentioned above, the experimental animal fixation observation device of this embodiment can achieve drug administration during imaging observation and can accurately control the drug dosage, which can solve the problems of easy activation of tissue cells due to exposure, uncontrollable drug solution concentration, and corrosion to the objective lens in the experiment.

[0056] In some embodiments, the experimental animal fixing observation device further includes an adjustment mechanism 4, which is connected to the body 1 and is used to adjust the height of the body 1. Thus, the height of the body 1 can be adjusted by the adjustment structure during the experimental preparation stage, so that the body 1 can be better fitted with the experimental object, and the body 1 can be ensured to be set horizontally, thereby avoiding experimental errors caused by the tilt of the body 1.

[0057] In some specific embodiments, the adjustment mechanism 4 includes an adjustment block 41 and an adjustment knob 42. The adjustment block 41 is provided with a threaded hole. The adjustment knob 42 has a stud that matches the threaded hole, and one end of the stud matches the body 1. Therefore, in the actual adjustment process, the adjustment of the body 1 can be achieved by only turning the adjustment knob 42, which is very convenient to operate.

[0058] Of course, in the embodiment of the present invention, the adjustment mechanism 4 can also be of other types. For example, the fixing frame 3 is provided with a plurality of installation slots arranged at intervals, and the cranial window for brain drug administration observation can be fitted in a certain installation slot according to actual needs.

[0059] In some specific embodiments, there are two adjustment mechanisms 4, and the two adjustment mechanisms 4 are respectively connected to the two ends of the body 1. Thus, it is ensured that the body 1 can be set horizontally during the adjustment of the cranial window for observing brain drug administration, and experimental errors caused by the tilt of the body 1 are avoided.

[0060] In the description of this specification, the description with reference to the terms "some embodiments", "other embodiments", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0061] The above contents are only preferred embodiments of the present invention. For ordinary technicians in this field, according to the concept of the present invention, there will be changes in the specific implementation methods and application scopes. The content of this specification should not be understood as limiting the present invention.

Claims

1. A cranial window for observing brain drug administration, characterized in that: include: A body (1), wherein the body (1) is provided with a protruding portion (11), an end surface of the protruding portion (11) is provided with a light-transmitting hole (111) and a first through hole (112), a peripheral surface of the protruding portion (11) is provided with a second through hole (113), the first through hole (112) and the second through hole (113) being used to cooperate with a drug delivery tube (51) of a drug delivery pump (5); a light-transmitting plate (2), the light-transmitting plate (2) being matched on the protruding portion (11) to cover the light-transmitting hole (111); A matching groove (114) arranged around the light-transmitting hole (111) is provided on the end surface of the protruding portion (11), and the light-transmitting plate (2) is matched in the matching groove (114); The main body (1) is also provided with a fixing hole (12), and a fixing piece for fixing the head of the experimental subject is inserted into the fixing hole (12).

2. The cranial window for observing brain drug administration according to claim 1, characterized in that: The light-transmitting plate (2) is interference-fitted with the matching groove (114), or the light-transmitting plate (2) is bonded in the matching groove (114).

3. The cranial window for observing brain drug administration according to claim 1, characterized in that: There are a plurality of fixing holes (12), and the plurality of fixing holes (12) are evenly spaced around the protruding portion (11).

4. The cranial window for observing brain drug administration according to claim 3, characterized in that: The light-transmitting plate (2) comprises a glass plate or a transparent resin plate.

5. A fixed observation device for experimental animals, characterized in that: It comprises a cranial window for observing brain drug administration, a fixing frame (3) and a drug administration pump (5) as described in any one of claims 1 to 4, wherein the drug administration pump (5) is connected to the drug administration tube (51), and the cranial window for observing brain drug administration is installed on the fixing frame (3).

6. The experimental animal fixed observation device according to claim 5, characterized in that: The experimental animal fixing and observation device further comprises an adjusting mechanism (4), wherein the adjusting mechanism (4) is connected to the main body (1), and the adjusting mechanism (4) is used to adjust the height of the main body (1).

7. The experimental animal fixed observation device according to claim 6, characterized in that: The regulating mechanism (4) comprises: An adjusting block (41), wherein the adjusting block (41) is provided with a threaded hole; An adjusting knob (42) is provided with a stud which cooperates with the threaded hole, and one end of the stud cooperates with the body (1).

8. The experimental animal fixed observation device according to claim 6, characterized in that: There are two adjusting mechanisms (4), and the two adjusting mechanisms (4) are respectively connected to two ends of the body (1).

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