An automatic sample adding and mixing device for spectrophotometry pretreatment

Abstract

The present invention provides an automatic sample adding and mixing device for spectrophotometric pretreatment, which mainly includes a base, a sample adding and sampling device for adding a buffer and sampling a sample cup after the buffer is added and mixed, a transmission and mixing device for transmitting the sample cup and a cuvette, a weighing device for measuring the overall weight of the sample cup, a reagent adding device for adding experimental reagents, a cleaning device for cleaning a sample adding and sampling needle and a reagent needle, a control system for controlling the above-mentioned devices, and a control terminal; the control terminal mainly exists in the form of control software running on a network computer. The sample adding and sampling device, the transmission and mixing device, the weighing device, the reagent adding device, the cleaning device, the control system, and the control terminal in the present invention are used in combination to realize automatic sample addition to the cuvette in the pretreatment part of spectrophotometry, which not only has high efficiency and accuracy and small error, but also reduces the cost of manual sample addition by professional experimenters, thereby saving costs.

Description

Technical Field

[0001] The invention mainly relates to the technical field of detection equipment, and in particular to an automatic sample adding and mixing device for spectrophotometry pretreatment. Background Art

[0002] Detection technology is one of the indispensable means in scientific experiments. The commonly used ultraviolet spectrophotometry is a qualitative, quantitative and structural analysis method based on the absorption characteristics of material molecules to electromagnetic waves of different wavelengths. As an optical detection device, the spectrophotometer is often used to detect and analyze the content of various elements in the sample.

[0003] Spectrophotometry is divided into two parts: pretreatment and detection. The pretreatment part accounts for about 80% of the entire process. However, in spectrophotometric detection experiments, manual operations are still required to complete the entire pretreatment process, including sample weighing, adding sample solution, oscillating and mixing, sample extraction, and adding reagents. Due to the large number of pretreatment steps, manual operation is not only inefficient but also inevitably causes operational errors, which in turn affect the test results. Therefore, there is a need for an automatic sample adding and mixing device for pretreatment, which can automatically complete the pretreatment work in spectrophotometry, thereby saving time, effort, and cost. Summary of the Invention

[0004] In order to solve the technical problems in spectrophotometry that the manual sample addition method has low efficiency, poor accuracy, errors in detection results, high labor costs, and cannot realize automated detection, the present invention provides an automatic sample addition and mixing device for spectrophotometry pretreatment.

[0005] The technical solution provided by the present invention is:

[0006] The present invention provides an automatic sample adding and mixing device for spectrophotometry pre-treatment, comprising a base, a power supply placed on the base, a transmission and mixing device for mixing samples, a sample adding and sampling device for adding a buffer, a reagent adding device for adding an experimental reagent, a weighing device for measuring the overall weight of a sample cup, a cleaning device for cleaning a sample adding and sampling needle and a reagent needle, a control terminal for collecting data, and a control system arranged above the base; the transmission and mixing device is fixed to the base via a support column; the sample adding and sampling device, the reagent adding device, the weighing device, and the cleaning device are arranged around the transmission and mixing device; the control system is used to control the transmission and mixing device, the sample adding and sampling device, the reagent adding device, the weighing device, and the cleaning device, and is also used to receive instructions issued by the control terminal; the control terminal exists in the form of operation control software in a network computer or a mobile phone.

[0007] Preferably, the transmission mixing device includes a first stepper motor, a first transmission belt, a rotating shaft, a tray and a tray bracket; the tray bracket includes the support column and a fixed platform; the support column is vertically installed on the base; the fixed platform is horizontally fixed to the top of the support column; an axial hole is opened in the center of the fixed platform; the rotating shaft is arranged through the axial hole; a first bearing (not shown in the figure) is arranged in the axial hole; the outer ring of the first bearing is embedded in the inner wall of the axial hole; the inner ring of the first bearing is embedded in the surface of the rotating shaft; the tray is arranged parallel to the fixed platform; the center of the tray is fixed to the top of the rotating shaft; the first stepper motor is installed below the fixed platform through an "L-shaped" fixing bracket, and its drive shaft is arranged vertically, and the drive shaft is connected to the bottom end of the rotating shaft through the first transmission belt.

[0008] Preferably, the tray is disc-shaped; a plurality of sample holes, a plurality of reagent holes and a plurality of cuvette holes are provided on the surface of the disc-shaped tray; the sample holes, the reagent holes and the cuvette holes are evenly distributed around the axis of the center of the disc-shaped tray; the distance from the center of the cuvette hole to the axis is a, the distance from the center of the sample hole to the axis is b, and the distance from the center of the reagent hole to the axis is c; a≦b≦c; a first connecting column is provided on the lower bottom surface at the edge of the tray; the first connecting column is provided perpendicular to the tray; the bottom end of the first connecting column is connected to an arc-shaped support plate; the arc-shaped support plate is provided between the tray and the fixed platform, and the arc-shaped support plate is provided directly below all the reagent holes; a second connecting column is also provided at the bottom of the tray; the bottom end of the second connecting column is connected to an annular support plate; the annular support plate is provided between the tray and the fixed platform, and the annular support plate is provided directly below all the cuvette holes.

[0009] Preferably, a heating disk is provided on the fixed platform; a temperature sensor is provided on the heating disk; a heating coil for heating is provided in the heating disk; the heating disk is fixed at the center of the fixed platform; a through hole for the passage of the rotating shaft is provided in the center of the heating disk; the heating disk is provided with an annular groove for heating the cuvette; and the annular support plate is arranged in the annular groove.

[0010] Preferably, the reagent adding device includes a first mounting seat, a plunger pump, a second stepping motor, a first rotating rod, a first rotating arm, and an "L-shaped" reagent needle; the first mounting seat includes a first lower plate, a first support plate and a first upper plate; the first lower plate is fixed on the base; the first upper plate is arranged directly above the first lower plate; the first support plate is used to connect the first upper plate and the first lower plate; the second stepping motor is fixed on the bottom of the first upper plate, and its drive shaft passes through the first upper plate; the first rotating rod can be rotatably passed through the first upper plate; the drive shaft of the second stepping motor and the first rotating rod are connected by a second transmission belt; the bottom of the first lower plate A third stepper motor is provided; the driving shaft of the third stepper motor passes through the first support plate and is provided with a first driving wheel at the end; a first driven wheel is provided directly above the first driving wheel; the first driving wheel and the first driven wheel are connected by a third transmission belt; a second bearing is sleeved on the bottom end of the first rotating rod; a first transmission plate is fixed to the outer ring of the second bearing; the first transmission plate is fixedly connected to one side of the third transmission belt; one end of the first rotating arm is fixed to the top end of the first rotating rod; the vertical section of the "L-shaped" reagent needle passes through the other end of the first rotating arm; a reagent tube is passed through the first rotating rod; one end of the reagent tube is connected to the horizontal section of the "L-shaped" reagent needle, and the other end is connected to the plunger pump.

[0011] Preferably, the sampling device includes a second mounting seat, a peristaltic pump, a fourth stepper motor, a second rotating rod, a second rotating arm, and an "L-shaped" sampling needle; the second mounting seat includes a second lower plate, a second support plate and a second upper plate; the second lower plate is fixed on the base; the second upper plate is located directly above the second lower plate; the second support plate is used to connect the second upper plate and the second lower plate; the fourth stepper motor is fixed on the bottom of the second upper plate, and its drive shaft passes through the second upper plate; the second rotating rod can rotatably pass through the second upper plate; the drive shaft of the fourth stepper motor and the second rotating rod are connected by a fourth transmission belt; the bottom of the second lower plate is provided with A fifth stepper motor; the driving shaft of the fifth stepper motor passes through the second support plate and is provided with a second driving wheel at the end; a second driven wheel is provided directly above the second driving wheel; the second driving wheel and the second driven wheel are connected by a fifth transmission belt; a third bearing is sleeved on the bottom end of the second rotating rod; a second transmission plate is fixed to the outer ring of the third bearing; the second transmission plate is fixedly connected to one side of the fifth transmission belt; one end of the second rotating arm is fixed to the top end of the second rotating rod; the vertical section of the "L-shaped" sampling needle passes through the other end of the second rotating arm; a sample liquid tube is passed through the second rotating rod; one end of the sample liquid tube is connected to the horizontal section of the "L-shaped" sampling needle, and the other end is connected to the peristaltic pump.

[0012] Preferably, the weighing device includes a weighing pan, a pressure sensor and a screw motor; the screw of the screw motor is arranged in a vertical direction; a slider is slidably provided on the screw of the screw motor; the upper end of the slider is connected to the pressure sensor, and the upper end of the pressure sensor is connected to the weighing pan; the fixed platform is provided with a sample slot through its plate surface; the weighing pan passes through the sample slot; the weighing pan is arranged directly below the sample hole.

[0013] Preferably, the cleaning device includes a cleaning tank installed on the fixed platform, a first diaphragm pump and a second diaphragm pump installed on the base; the first diaphragm pump is provided with a waste liquid pipe and a discharge pipe; the waste liquid pipe connects the cleaning tank and the first diaphragm pump; one end of the discharge pipe is connected to the first diaphragm pump, and the other end is connected to the waste liquid tank;

[0014] The second diaphragm pump is provided with an inlet pipe and an outlet pipe; one end of the inlet pipe is connected to the clean water tank, and the other end is connected to the second diaphragm pump, and the outlet pipe is connected to the second diaphragm pump and the cleaning tank; a branch pipe is connected to the outlet pipe; an electromagnetic valve is provided on the branch pipe; the end of the branch pipe is connected to the plunger pump;.

[0015] Preferably, the control system includes a control main board and a data exchanger connected to the control main board; the control terminal is connected to the control main board by wireless connection or electrical connection; the data exchanger includes a temperature control module and a drive module; the temperature control module is connected to the temperature sensor to control the temperature of the heating plate; the first drive module is used to control the operation of the first stepper motor, the second stepper motor, the third stepper motor, the fourth stepper motor, the fifth stepper motor, the plunger pump, the peristaltic pump, the diaphragm pump and the screw motor.

[0016] Beneficial effects of the present invention:

[0017] The automatic sample adding and mixing device for spectrophotometry pretreatment provided by the present invention is used in conjunction with a sample adding and sampling device, a transmission and mixing device, a weighing device, a reagent adding device, a cleaning device, a control system and a control terminal to realize automatic addition of cuvette samples and reagents in the pretreatment part of spectrophotometry, which not only has high efficiency and accuracy and small error, but also reduces the cost of manual sample adding by professional experimenters, thereby saving costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention and its features, configurations, and advantages will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings. Like reference numerals indicate like parts throughout the drawings. The drawings are not drawn to scale, emphasis instead being placed on illustrating the subject matter of the present invention.

[0019] Figure 1 It is a three-dimensional structural diagram of the present invention;

[0020] Figure 2 A three-dimensional structural diagram of the present invention from another angle;

[0021] Figure 3 It is the front view of the present invention;

[0022] Figure 4 A top view of the fixed platform of the present invention;

[0023] Figure 5 This is a three-dimensional structural diagram of a weighing device according to the present invention;

[0024] Figure 6 This is a three-dimensional structural diagram of the transmission and mixing device of the present invention;

[0025] Figure 7 This is a three-dimensional structural diagram of a reagent adding device according to the present invention; DETAILED DESCRIPTION

[0026] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of this application can be combined with each other. It should be noted that the terms used in the present invention are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to this application.

[0027] It should be understood that when the terms “include” and / or “comprising” are used in this specification, they specify the presence of features, steps, operations, devices, components and / or their combinations.

[0028] If the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention.

[0029] The terms "first", "second" and "third" are used for descriptive purposes only and should not be understood as indicating or implying relative importance.

[0030] Unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be interpreted broadly. For example, they can refer to fixed, removable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediary; and internal communication between two components. Those skilled in the art will understand the specific meanings of these terms in the present invention.

[0031] The technical solutions in the embodiments of the present invention are described below in conjunction with the accompanying drawings in the embodiments of the present invention. It is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Therefore, the detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making any creative efforts shall fall within the scope of protection of the present invention.

[0032] Example 1

[0033] In order to solve the technical problems in spectrophotometry, the manual sampling method has low efficiency, poor accuracy, errors in detection results, high labor costs, and cannot realize automated detection.

[0034] An embodiment of the present invention provides an automatic sample adding and mixing device for spectrophotometric pretreatment, comprising a base 1, a power supply 2 placed on the base 1 (for powering the entire device), a transmission and mixing device 3 for mixing samples, a sample adding and sampling device 4 for adding a buffer, a reagent adding device 5 for adding an experimental reagent, a weighing device 6 for measuring the overall weight of a sample cup, a cleaning device 7 for cleaning a sample adding and sampling needle 406 and a reagent needle 506, a control terminal 8 for collecting data, and a control system 9 located above the base 1; the transmission and mixing device 3 is fixed to the base 1 via a support column 10; the sample adding and sampling device 4, the reagent adding device 5, the weighing device 6, and the cleaning device 7 are arranged around the transmission and mixing device 3; the control system 9 is used to control the transmission and mixing device 3, the sample adding and sampling device 4, the reagent adding device 5, the weighing device 6, and the cleaning device 7, and the control system 9 is also used to receive instructions issued by the control terminal 8; the control terminal 8 exists in the form of operation control software in a network computer or a mobile phone.

[0035] In this embodiment, the transmission mixing device 3 preferably includes a first stepper motor 301, a first transmission belt 302, a rotating shaft 303, a tray 304 and a tray bracket 305; the tray bracket 305 includes a support column 10 and a fixed platform 11; the support column 10 is vertically installed on the base 1; the fixed platform 11 is horizontally fixed to the top of the support column 10; an axial hole is opened in the center of the fixed platform 11; the rotating shaft 303 is arranged through the axial hole; a first bearing is arranged in the axial hole; the outer ring of the first bearing is embedded in the inner wall of the axial hole; the inner ring of the first bearing is embedded in the surface of the rotating shaft 303, so the tray 304 will rotate under the action of the first stepper motor 301, while the fixed platform 11 will not; The disk 304 is arranged parallel to the fixed platform 11; the center of the tray 304 is fixed to the top of the rotating shaft 303; the first stepper motor 301 is installed below the fixed platform 11 through the "L-shaped" fixing frame 12, and its drive shaft is arranged vertically. The drive shaft is connected to the bottom end of the rotating shaft 303 through the first transmission belt 302. The first stepper motor 301 receives instructions from the driving module 33 and drives the rotating shaft and the tray 303 installed on the rotating shaft to move synchronously through the first transmission belt 302; a driving wheel can be added to the driving shaft, and a driven wheel can be added to the bottom of the rotating shaft. The first transmission belt 302 can act on the driving wheel and the driven wheel to realize the synchronous movement of the driving shaft and the rotating shaft.

[0036] In this embodiment, the tray 304 is preferably in the shape of a disk; a plurality of sample wells 13, a plurality of reagent wells 14 and a plurality of cuvette wells 15 are provided on the surface of the disk-shaped tray 304, and the sample cups, reagent bottles and cuvettes are placed in the sample wells 13, the plurality of reagent wells 14 and the plurality of cuvette wells 15 respectively; the sample wells 13, the reagent wells 14 and the cuvette wells 15 are evenly distributed around the axis of the center of the disk-shaped tray 304; the distance from the center of the cuvette well 15 to the axis is a, the distance from the center of the sample well 13 to the axis is b, and the distance from the center of the reagent well 14 to the axis is c; a≦b≦c, this is because in the spectrophotometric experiment, the diameter of the sample cup is generally larger than the diameter of the cuvette aperture. To ensure that the number of sample cups and cuvettes is consistent, the sample well 13 for placing the sample cup must be set on the outer circle of the tray 304, and the reagent well 14 is set on the outer circle of the tray 304. The outermost circle of 304 is for cooperating with the use of reagent needle 506; a first connecting column 16 is provided on the lower bottom surface at the edge of the tray 304; the first connecting column 16 is arranged perpendicular to the tray 304; the bottom end of the first connecting column 16 is connected to the arc-shaped support plate 17; the arc-shaped support plate 17 is arranged between the tray 304 and the fixed platform 11, and the arc-shaped support plate 17 is arranged directly below all the reagent holes 14. The arc-shaped support plate 17 can be used to support each reagent bottle so that each reagent bottle will not fall and maintain it in the corresponding position; a second connecting column 18 is also provided at the bottom of the tray 304; the bottom end of the second connecting column 18 is connected to the annular support plate 19; the annular support plate 19 is arranged between the tray 304 and the fixed platform 11, and the annular support plate 19 is arranged directly below all the cuvette holes 15. Similar to the function of the arc-shaped support plate 17, the added annular tray 19 can prevent the cuvette from falling.

[0037] In this embodiment, preferably, a heating disk 20 is provided on the fixed platform 11; a temperature sensor is provided on the heating disk 20; a heating coil for heating is provided in the heating disk 20 (not shown in the figure); the heating disk 20 is fixed at the center of the fixed platform 11. The ambient temperature is low in winter, which will affect the activity of various reagents. In order to avoid the inconvenience caused by the ambient temperature to the spectrophotometric experiment, a heating disk and a cuvette are used to increase the temperature; a through hole for the passage of the rotating shaft 303 is provided in the center of the heating disk 20; the heating disk 20 is provided with an annular groove 21 for heating the cuvette; the annular support plate 19 is arranged in the annular groove 21, and each cuvette is heated and kept warm in the groove of the annular groove 21.

[0038] In this embodiment, the reagent adding device 5 preferably includes a first mounting seat 501, a plunger pump 502, a second stepping motor 503, a first rotating rod 504, a first rotating arm 505, and an "L-shaped" reagent needle 506; the first mounting seat 501 includes a first lower plate, a first support plate and a first upper plate; the first lower plate is fixed on the base 1; the first upper plate is arranged directly above the first lower plate; the first support plate is used to connect the first upper plate and the first lower plate; the second stepping motor 503 is fixed at the bottom of the first upper plate, and its drive shaft passes through the first upper plate; the first rotating rod 504 can rotatably pass through the first upper plate; the drive shaft of the second stepping motor 503 and the first rotating rod 504 are connected by a second transmission belt 22; the bottom of the first lower plate is provided with a third stepper motor 503. Step motor 23; the driving shaft of the third stepper motor 23 passes through the first support plate and is provided with a first driving wheel 24 at the end; a first driven wheel 25 is provided directly above the first driving wheel 24; the first driving wheel 24 and the first driven wheel 25 are connected by a third transmission belt 26; a second bearing is sleeved on the bottom end of the first rotating rod 504; the outer ring of the second bearing is fixed with a first transmission plate 28; the first transmission plate 28 is fixedly connected to one side of the third transmission belt 26; one end of the first rotating arm 505 is fixed to the top of the first rotating rod 504; the vertical section of the "L-shaped" reagent needle 506 passes through the other end of the first rotating arm 505; a reagent tube 27 is passed through the first rotating rod 504; one end of the reagent tube 27 is connected to the horizontal section of the "L-shaped" reagent needle 506, and the other end is connected to the plunger pump 502. The second stepper motor 503 and the third stepper motor 23 receive instructions from the driving module 33, and the second stepper motor 503 realizes the synchronous movement of the first rotating rod 504 and the driving shaft through the second transmission belt 22; the lifting and lowering of the first rotating rod 504 is realized by the third stepper motor 23: the relative positions of the first driving wheel 24 and the first driven wheel 25 on the driving shaft of the third stepper motor 23 remain unchanged. Under the action of friction, the third transmission belt 26 drives the first transmission plate 28 installed thereon to move up and down, thereby driving the second bearing and the first rotating rod 504 installed on the second bearing to rise and fall; the reagent needle 506 is used in conjunction with the reagent tube 17, and the plunger pump 502 absorbs the reagent in the reagent bottle and retains the reagent at the front end of the reagent tube 17, so as to facilitate the subsequent injection of reagent into each cuvette.

[0039] In this embodiment, the sampling device 4 preferably includes a second mounting base 401, a peristaltic pump 402, a fourth stepper motor 403, a second rotating rod 404, a second rotating arm 405, and an "L-shaped" sampling needle 406; the second mounting base 401 includes a second lower plate, a second support plate and a second upper plate; the second lower plate is fixed on the base 1; the second upper plate is located directly above the second lower plate; the second support plate is used to connect the second upper plate and the second lower plate; the fourth stepper motor 403 is fixed at the bottom of the second upper plate, and its drive shaft passes through the second upper plate; the second rotating rod 404 can rotatably pass through the second upper plate; the drive shaft of the fourth stepper motor 403 and the second rotating rod 404 are connected by a fourth transmission belt; the second lower plate A fifth stepper motor is provided at the bottom of the plate; the driving shaft of the fifth stepper motor passes through the second support plate and is provided with a second driving wheel at the end; a second driven wheel is provided directly above the second driving wheel; the second driving wheel and the second driven wheel are connected by a fifth transmission belt; a third bearing is provided at the bottom end of the second rotating rod 404; the outer ring of the third bearing is fixed with a second transmission plate; the second transmission plate is fixedly connected to one side of the fifth transmission belt; one end of the second rotating arm 405 is fixed to the top of the second rotating rod 404; the vertical section of the "L-shaped" sampling needle 406 passes through the other end of the second rotating arm 405; a sample liquid tube 29 is provided inside the second rotating rod 404; one end of the sample liquid tube 29 is connected to the horizontal section of the "L-shaped" sampling needle 406, and the other end is connected to the peristaltic pump 402. The fourth stepper motor and the fifth stepper motor receive instructions from the driving module 33, and the fourth stepper motor realizes the synchronous movement of the drive shaft and the first rotating rod 404 through the fourth transmission belt; the lifting and lowering of the first rotating rod 404 is realized by the fifth stepper motor: the relative positions of the second active wheel and the second driven wheel on the fifth stepper motor drive shaft remain unchanged, and under the action of friction, the fifth transmission belt drives the second transmission plate installed thereon to move up and down, thereby driving the third bearing and the first rotating rod 404 installed on the third bearing to rise and fall; the sampling needle 406 is used in conjunction with the sample liquid tube 29, and the peristaltic pump 402 absorbs the sample liquid in the sample cup and retains the sample liquid at the front end of the sample liquid tube 29, so as to facilitate the subsequent injection of the sample liquid into the corresponding cuvette.

[0040] In this embodiment, the weighing device 6 preferably includes a weighing pan 601, a pressure sensor 602, and a screw motor 603. The screw of the screw motor 603 is arranged in a vertical direction. A slider 30 is slidably mounted on the screw of the screw motor 603. The upper end of the slider 30 is connected to the pressure sensor 602, and the upper end of the pressure sensor 602 is connected to the weighing pan 601. The fixed platform 11 has a sample slot 31 disposed through its surface. The weighing pan 601 passes through the sample slot 31 and is positioned directly below the sample hole 13. During the spectrophotometric experiment, sample cup No. 1 containing a sample is weighed. If the weight of the sample cup falls within the set range, the tray 304 rotates and the next sample cup containing a sample is weighed.

[0041] In this embodiment, the cleaning device 7 preferably includes a cleaning tank 701 installed on a fixed platform, a first diaphragm pump 702 and a second diaphragm pump 703 installed on a base; the first diaphragm pump 702 is provided with a waste liquid pipe 34 and a discharge pipe 36; the waste liquid pipe 34 connects the cleaning tank 701 and the first diaphragm pump 702; one end of the discharge pipe 36 is connected to the first diaphragm pump 702, and the other end is connected to the waste liquid tank 40;

[0042] The second diaphragm pump 703 is provided with an inlet pipe 38 and an outlet pipe 39; one end of the inlet pipe 38 is connected to the clean water tank 41, and the other end is connected to the second diaphragm pump 703, and the outlet pipe 39 connects the second diaphragm pump 703 and the cleaning tank 701; the outlet pipe 39 is connected to a branch pipe 37; the branch pipe 37 is provided with an electromagnetic valve 35; the end of the branch pipe 37 is connected to the plunger pump 502.

[0043] The second diaphragm pump 703 draws clean water from the clean water tank 41 into the clean water tank 701, and the first diaphragm pump 702 pumps the contaminated clean water into the waste liquid tank 40. The clean water in the cleaning tank 701 is replaced immediately, and the degree of clean water contamination can be reduced to a minimum; wherein, the first diaphragm pump 702 and the second diaphragm pump 703 are started at the same time, and the total operating time of the first diaphragm pump 702 is ≧ the total operating time of the second diaphragm pump 703, so as to prevent the clean water in the cleaning tank 701 from overflowing; the outer wall of the reagent needle 506 only needs to be extended into the cleaning tank 701 for cleaning, and the cleaning method of its inner wall is as follows: the solenoid valve 37 is opened, and clean water is passed through the branch pipe 37, and the plunger pump 502 pumps the clean water in the branch pipe 37 into the interior of the reagent needle 506 and discharges it into the cleaning tank 701, thereby cleaning the inner wall of the reagent needle 506.

[0044] Preferably in this embodiment, the control system 9 includes a control main board 901 and a data exchanger 902 connected to the control main board 901; the control terminal 8 is connected to the control main board 901 by wireless connection or electrical connection; the data exchanger 902 includes a temperature control module 32 and a drive module 33; the temperature control module 32 is connected to a temperature sensor for controlling the temperature of the heating plate 20; the first drive module 33 is used to control the operation of the first stepper motor 301, the second stepper motor 503, the third stepper motor 23, the fourth stepper motor 403, the fifth stepper motor, the plunger pump 502, the peristaltic pump 402, the diaphragm pump 702 and the screw motor 603, so as to control the use of the transmission mixing device 3, the sampling device 4, the reagent adding device 5, the weighing device 6 and the cleaning device 7.

[0045] The working steps of the automatic sample adding and mixing device for spectrophotometric pre-treatment involved in this embodiment are as follows:

[0046] 1. The weighing pan rises to its highest point, and the sample is placed in the sample cup. The sample cup is then placed in the sample hole 13 marked with position 1 above the weighing pan 601. The sample cup falls on the weighing pan 601. The sample cup in the sample hole 13 is marked as sample cup 1. The sample cup 1 with the sample is weighed. If the weight of the sample cup is within the set range, the tray 304 rotates and the next sample cup with the sample is weighed. If the weight of the sample cup is not within the set range, the tray 304 stops rotating. After obtaining the weights of all the sample cups, the weighing pan 601 descends to its initial position, and the tray 304 rotates the sample cup 1 to the position to be filled with liquid. The sample collection needle 406 rotates to the cleaning tank 701 for cleaning, and after completion, it rises and stays above the cleaning tank 701.

[0047] 2. The second rotating rod rotates, driving the sample adding needle 406 to move to the top of the sample cup, and adding buffer to all the sample cups in turn. The amount of buffer added is based on the actual weight of the sample in each sample cup, that is, it is added proportionally. After all additions are completed, the sample adding needle 406 is on standby above the tray 304; the tray 304 rotates clockwise and counterclockwise alternately to fully mix the sample and buffer in the sample cup; the sample adding needle 406 moves to the top of the No. 1 sample cup, descends to a certain height, and absorbs No. 1 The sample liquid in the sample cup; then the sampling needle 406 rises and rotates to the top of the No. 1 cuvette, and injects the sample liquid into the No. 1 cuvette; the sampling needle 406 moves to the cleaning tank 701 for cleaning; the sample liquid in each sample cup is injected into each cuvette respectively until the sample liquid in all sample cups is extracted; the tray 304 is reset to the initial position; the sampling needle 406 moves to the cleaning tank 701 for cleaning, and after cleaning is completed, the sampling needle 406 stands by above the cleaning tank 701;

[0048] 3. Clean the reagent needle 506. After cleaning, the reagent needle 506 is on standby above the cleaning pool 701. Reagent bottles containing different reagents are placed in each reagent well 14 respectively. The first rotating rod drives the reagent needle 506 to move above one of the reagent bottles, sucks a certain amount of reagent in the reagent bottle, and sucks all the reagent required for the time into the reagent tube 27 at one time. The reagent needle 506 moves to the top of the No. 1 cuvette and injects the reagent into the No. 1 cuvette. The position of the reagent needle 506 remains unchanged. The tray 304 is rotated, and a one-suck-multiple-shot method is adopted. The reagent is injected into each cuvette in turn. After all the additions are completed, the reagent needle 506 rotates to the cleaning tank 701 for cleaning. After cleaning is completed, the first rotating rod drives the reagent needle 506 to move, absorb the second reagent, and inject it into the cuvettes one by one. After all the second reagents are added, the reagent needle 506 rotates to the cleaning tank 701 for cleaning. Similarly, the reagents in the remaining reagent bottles are injected into each cuvette one by one. After each reagent is added to the tray 304, whether the tray 304 is rotated alternately clockwise and counterclockwise to shake it evenly can be specified according to specific experimental requirements.

[0049] The above embodiments are merely illustrative of the principles and effects of this patent application and are not intended to limit this patent application. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of this patent application. Therefore, all equivalent modifications or alterations made by persons of ordinary skill in the art without departing from the spirit and technical concepts disclosed in this patent application shall be covered by the claims of this patent application.

Claims

1. An automatic sample adding and mixing device for spectrophotometric pretreatment, characterized in that: include: A base, a power supply placed on the base, a transmission mixing device for mixing samples, a sampling device for adding buffers, a reagent adding device for adding experimental reagents, a weighing device for measuring the overall weight of the sample cup, a cleaning device for cleaning the sampling needle and the reagent needle, a control terminal for collecting data, and a control system located above the base; The transmission and mixing device is fixed to the base via a support column; the sample adding and sampling device, the reagent adding device, the weighing device, and the cleaning device are arranged around the transmission and mixing device; the control system is used to control the transmission and mixing device, the sample adding and sampling device, the reagent adding device, the weighing device, and the cleaning device, and is also used to receive instructions from the control terminal; the control terminal exists in the form of a network computer or operating control software in a mobile phone; The transmission mixing device includes a tray; the tray is disc-shaped; a plurality of sample wells, a plurality of reagent wells, and a plurality of cuvette wells are provided on the surface of the disc-shaped tray; the sample wells, the reagent wells, and the cuvette wells are evenly distributed around the axis of the center of the disc-shaped tray; A first connecting column is provided on the lower bottom surface of the edge of the tray; the first connecting column is arranged perpendicular to the tray; the bottom end of the first connecting column is connected to an arc-shaped support plate; the arc-shaped support plate is arranged between the tray and the fixed platform, and the arc-shaped support plate is arranged directly below all the reagent wells; The cleaning device includes a cleaning tank installed on the fixed platform, a first diaphragm pump and a second diaphragm pump installed on the base; the first diaphragm pump is provided with a waste liquid pipe and a discharge pipe; the waste liquid pipe connects the cleaning tank and the first diaphragm pump; one end of the discharge pipe is connected to the first diaphragm pump, and the other end is connected to the waste liquid tank; The second diaphragm pump is provided with an inlet pipe and an outlet pipe; one end of the inlet pipe is connected to the clean water tank, and the other end is connected to the second diaphragm pump, and the outlet pipe is connected to the second diaphragm pump and the cleaning tank; a branch pipe is connected to the outlet pipe; an electromagnetic valve is provided on the branch pipe; the end of the branch pipe is connected to the plunger pump.

2. The automatic sample adding and mixing device for spectrophotometric pretreatment according to claim 1, characterized in that: The transmission and mixing device includes a first stepper motor, a first transmission belt, a rotating shaft and a tray support; the tray support includes the support column and a fixed platform; the support column is vertically mounted on the base; the fixed platform is horizontally fixed to the top of the support column; An axial hole is provided at the center of the fixed platform; the rotating shaft is arranged through the axial hole; a first bearing is arranged in the axial hole; the outer ring of the first bearing is embedded in the inner wall of the axial hole; the inner ring of the first bearing is embedded in the surface of the rotating shaft; the tray is arranged parallel to the fixed platform; the center of the tray is fixed to the top of the rotating shaft; the first stepper motor is installed below the fixed platform through an "L-shaped" fixing bracket, and its drive shaft is arranged vertically, and the drive shaft is connected to the bottom end of the rotating shaft through the first transmission belt.

3. The automatic sample adding and mixing device for spectrophotometric pretreatment according to claim 2, characterized in that: The distance from the center of the cuvette hole to the axis is a, the distance from the center of the sample hole to the axis is b, and the distance from the center of the reagent hole to the axis is c; a≦b≦c; A second connecting column is further provided at the bottom of the tray; the bottom end of the second connecting column is connected to an annular support plate; the annular support plate is provided between the tray and the fixed platform, and the annular support plate is provided directly below all the cuvette holes.

4. The automatic sample adding and mixing device for spectrophotometric pretreatment according to claim 3, characterized in that: A heating disk is provided on the fixed platform; a temperature sensor is provided on the heating disk; a heating coil for heating is provided inside the heating disk; the heating disk is fixed at the center of the fixed platform; a through hole for the passage of the rotating shaft is provided at the center of the heating disk; the heating disk is provided with an annular groove for heating the cuvette; the annular support plate is arranged in the annular groove.

5. The automatic sample adding and mixing device for spectrophotometric pretreatment according to claim 4, characterized in that: The reagent adding device includes a first mounting seat, a plunger pump, a second stepping motor, a first rotating rod, a first rotating arm, and an "L-shaped" reagent needle; the first mounting seat includes a first lower plate, a first support plate, and a first upper plate; the first lower plate is fixedly mounted on the base; the first upper plate is located directly above the first lower plate; the first support plate is used to connect the first upper plate and the first lower plate; The second stepper motor is fixedly mounted on the bottom of the first upper panel, and its drive shaft is disposed through the first upper panel; the first rotating rod is rotatably disposed through the first upper panel; the drive shaft of the second stepper motor and the first rotating rod are connected by a second transmission belt; A third stepper motor is provided at the bottom of the first lower plate; a drive shaft of the third stepper motor is provided through the first support plate and a first driving wheel is provided at the end thereof; a first driven wheel is provided directly above the first driving wheel; the first driving wheel and the first driven wheel are connected by a third transmission belt; A second bearing is sleeved on the bottom end of the first rotating rod; a first transmission plate is fixed to the outer ring of the second bearing; and the first transmission plate is fixedly connected to one side of the third transmission belt; One end of the first rotating arm is fixed to the top of the first rotating rod; the vertical section of the "L-shaped" reagent needle is arranged through the other end of the first rotating arm; a reagent tube is arranged through the first rotating rod; one end of the reagent tube is connected to the horizontal section of the "L-shaped" reagent needle, and the other end is connected to the plunger pump.

6. The automatic sample adding and mixing device for spectrophotometric pretreatment according to claim 5, characterized in that: The sample addition and sampling device includes a second mounting seat, a peristaltic pump, a fourth stepping motor, a second rotating rod, a second rotating arm, and an "L-shaped" sample addition and sampling needle; the second mounting seat includes a second lower plate, a second support plate, and a second upper plate; the second lower plate is fixedly mounted on the base; the second upper plate is located directly above the second lower plate; the second support plate is used to connect the second upper plate and the second lower plate; The fourth stepper motor is fixedly mounted on the bottom of the second upper plate, and its drive shaft is disposed through the second upper plate; the second rotating rod is rotatably disposed through the second upper plate; the drive shaft of the fourth stepper motor and the second rotating rod are connected by a fourth transmission belt; A fifth stepper motor is provided at the bottom of the second lower plate; a drive shaft of the fifth stepper motor is provided through the second support plate and a second driving wheel is provided at the end thereof; a second driven wheel is provided directly above the second driving wheel; the second driving wheel and the second driven wheel are connected by a fifth transmission belt; A third bearing is sleeved on the bottom end of the second rotating rod; a second transmission plate is fixedly mounted on the outer ring of the third bearing; and the second transmission plate is fixedly connected to one side of the fifth transmission belt; One end of the second rotating arm is fixed to the top of the second rotating rod; the vertical section of the "L-shaped" sampling needle is arranged through the other end of the second rotating arm; a sample liquid tube is arranged through the second rotating rod; one end of the sample liquid tube is connected to the horizontal section of the "L-shaped" sampling needle, and the other end is connected to the peristaltic pump.

7. The automatic sample adding and mixing device for spectrophotometric pretreatment according to claim 6, characterized in that: The weighing device includes a weighing pan, a pressure sensor and a screw motor; the screw of the screw motor is arranged in a vertical direction; a slider is slidably provided on the screw of the screw motor; the upper end of the slider is connected to the pressure sensor, and the upper end of the pressure sensor is connected to the weighing pan; The fixed platform is provided with a sample slot running through its plate surface; the weighing pan passes through the sample slot; and the weighing pan is arranged just below the sample hole.

8. The automatic sample adding and mixing device for spectrophotometry pretreatment according to claim 7, characterized in that: A placement table with a placement hole is fixedly installed on the side wall of the fixed platform; the cleaning pool is placed in the placement hole.

9. The automatic sample adding and mixing device for spectrophotometric pretreatment according to claim 8, characterized in that: The control system includes a control mainboard and a data exchanger connected to the control mainboard; the control terminal is connected to the control mainboard in a wireless or electrical manner; the data exchanger includes a temperature control module and a drive module; The temperature control module is connected to the temperature sensor and is used to control the temperature of the heating plate; the first drive module is used to control the operation of the first stepper motor, the second stepper motor, the third stepper motor, the fourth stepper motor, the fifth stepper motor, the plunger pump, the peristaltic pump, the diaphragm pump and the screw motor.

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