Zein composite particle, delivery system, preparation method and application

Abstract

The invention discloses a zein composite particle, a delivery system, a preparation method and application, which belong to the technical field of functional composite materials. The protein composite particles are formed by zein and a second component in a solution through electrostatic interaction and covalent bond interaction, and the particle size of the protein composite particles is 100-1000 nm. The prepared particles can be used for preparing Pickering emulsion, drug carriers and microcapsules, and have excellent pH stability and emulsion stability. The method can be further applied to the fields of food, cosmetics, pharmacy and the like.

Description

technical field

[0001] The invention relates to a preparation method of a composite material, in particular to a zein composite particle, a preparation method and its application in a carrier system, and belongs to the field of functional composite materials. Background technique

[0002] Rising health care costs, aspirations for a better quality of life, and increasing life expectancy have led to an increasing demand for food over the past few decades. The relationship between eating habits and health status has been receiving a lot of attention from the scientific community. Bioactive substances have the function of promoting health, and are often treated by injection or oral administration. If they are added to food as dietary supplements, they can give food more functional properties.

[0003] However, some bioactive substances are relatively sensitive to the environment, so they need a delivery system. Common delivery systems include: emulsion, liposome, microcapsule, ...

Examples

Embodiment 1

[0041] (1) Preparation of zein solution: Dissolve NaOH in deionized water to prepare 100ml of strong alkaline solution with pH=13, add 0.5g zein (cas:9010-66-6, sigma), and sonicate for 10s A strong alkaline solution of zein was obtained. Solution A is obtained. 0.25g NaIO4 oxidized cellulose was dissolved in 100ml deionized water to obtain dispersion B.

[0042] (2) Synthesis of composite particles: uniformly mix solution A and dispersion B, sonicate for 20s, then adjust pH=13, react at 80°C for 4 hours, adjust pH=7 to obtain zein / cellulose composite particles Dispersion; centrifuged, washed, freeze-dried Composite particle 1 , The measured particle diameters of the particles at pH=5, 7, and 9 are listed in Table 1.

[0043] (3) Preparation of Pickering emulsion: the Composite particle 1 . Water and corn oil are prepared by homogenizing at 8000r / min for 1min with a weight ratio of 0.02:4:6, 0.25:5:5, 0.03:6:4 Emulsion system 1 .

Embodiment 2

[0045] (1) Preparation of zein solution: Dissolve NaOH in deionized water to prepare 100 ml of strong alkaline solution with pH=13, add 0.50 g of zein (cas:9010-66-6, sigma), and sonicate for 10 s A strong alkaline solution of zein was obtained. Solution A is obtained. 0.50gNaIO 4 Oxidized cellulose was dissolved in 100 ml of deionized water to obtain dispersion B.

[0046] (2) Synthesis of composite particles: uniformly mix solution A and dispersion B, sonicate for 20s, then adjust pH=13, react at 80°C for 4 hours, adjust pH=7 to obtain zein / cellulose composite particles Dispersion; centrifuged, washed, freeze-dried Composite particles 2 , The measured particle diameters of the particles at pH=5, 7, and 9 are listed in Table 1.

[0047] (3) Preparation of Pickering emulsion: the Composite particles 2. The weight ratio of water and corn oil is 0.02:4:6, 0.25:5:5, 0.03:6:4 and prepared by homogenizing at 8000r / min for 1min Emulsion system 2 .

Embodiment 3

[0049] (1) Preparation of zein solution: Dissolve NaOH in deionized water to prepare 100 ml of strong alkaline solution with pH=13, add 0.50 g of zein (cas:9010-66-6, sigma), and sonicate for 10 s A strong alkaline solution of zein was obtained. Solution A is obtained. 1.00g NaIO4 oxidized cellulose was dissolved in 100ml deionized water to obtain dispersion B.

[0050] (2) Synthesis of composite particles: uniformly mix solution A and dispersion B, sonicate for 20s, then adjust pH=13, react at 80°C for 4 hours, adjust pH=7 to obtain zein / cellulose composite particles Dispersion; centrifuged, washed, freeze-dried Composite particles 3 , The measured particle diameters of the particles at pH=5, 7, and 9 are listed in Table 1.

[0051] (3) Preparation of Pickering emulsion: the Composite particles 3 . Water and corn oil are prepared by homogenizing at 8000r / min for 1min with a weight ratio of 0.02:4:6, 0.25:5:5, 0.03:6:4 Emulsion system 3 .

technical field

[0001] The invention relates to a preparation method of a composite material, in particular to a zein composite particle, a preparation method and its application in a carrier system, and belongs to the field of functional composite materials. Background technique

[0002] Rising health care costs, aspirations for a better quality of life, and increasing life expectancy have led to an increasing demand for food over the past few decades. The relationship between eating habits and health status has been receiving a lot of attention from the scientific community. Bioactive substances have the function of promoting health, and are often treated by injection or oral administration. If they are added to food as dietary supplements, they can give food more functional properties.

[0003] However, some bioactive substances are relatively sensitive to the environment, so they need a delivery system. Common delivery systems include: emulsion, liposome, microcapsule, ...

Examples

Embodiment 1

[0041] (1) Preparation of zein solution: Dissolve NaOH in deionized water to prepare 100ml of strong alkaline solution with pH=13, add 0.5g zein (cas:9010-66-6, sigma), and sonicate for 10s A strong alkaline solution of zein was obtained. Solution A is obtained. 0.25g NaIO4 oxidized cellulose was dissolved in 100ml deionized water to obtain dispersion B.

[0042] (2) Synthesis of composite particles: uniformly mix solution A and dispersion B, sonicate for 20s, then adjust pH=13, react at 80°C for 4 hours, adjust pH=7 to obtain zein / cellulose composite particles Dispersion; centrifuged, washed, freeze-dried Composite particle 1 , The measured particle diameters of the particles at pH=5, 7, and 9 are listed in Table 1.

[0043] (3) Preparation of Pickering emulsion: the Composite particle 1 . Water and corn oil are prepared by homogenizing at 8000r / min for 1min with a weight ratio of 0.02:4:6, 0.25:5:5, 0.03:6:4 Emulsion system 1 .

Embodiment 2

[0045] (1) Preparation of zein solution: Dissolve NaOH in deionized water to prepare 100 ml of strong alkaline solution with pH=13, add 0.50 g of zein (cas:9010-66-6, sigma), and sonicate for 10 s A strong alkaline solution of zein was obtained. Solution A is obtained. 0.50gNaIO 4 Oxidized cellulose was dissolved in 100 ml of deionized water to obtain dispersion B.

[0046] (2) Synthesis of composite particles: uniformly mix solution A and dispersion B, sonicate for 20s, then adjust pH=13, react at 80°C for 4 hours, adjust pH=7 to obtain zein / cellulose composite particles Dispersion; centrifuged, washed, freeze-dried Composite particles 2 , The measured particle diameters of the particles at pH=5, 7, and 9 are listed in Table 1.

[0047] (3) Preparation of Pickering emulsion: the Composite particles 2. The weight ratio of water and corn oil is 0.02:4:6, 0.25:5:5, 0.03:6:4 and prepared by homogenizing at 8000r / min for 1min Emulsion system 2 .

Embodiment 3

[0049] (1) Preparation of zein solution: Dissolve NaOH in deionized water to prepare 100 ml of strong alkaline solution with pH=13, add 0.50 g of zein (cas:9010-66-6, sigma), and sonicate for 10 s A strong alkaline solution of zein was obtained. Solution A is obtained. 1.00g NaIO4 oxidized cellulose was dissolved in 100ml deionized water to obtain dispersion B.

[0050] (2) Synthesis of composite particles: uniformly mix solution A and dispersion B, sonicate for 20s, then adjust pH=13, react at 80°C for 4 hours, adjust pH=7 to obtain zein / cellulose composite particles Dispersion; centrifuged, washed, freeze-dried Composite particles 3 , The measured particle diameters of the particles at pH=5, 7, and 9 are listed in Table 1.

[0051] (3) Preparation of Pickering emulsion: the Composite particles 3 . Water and corn oil are prepared by homogenizing at 8000r / min for 1min with a weight ratio of 0.02:4:6, 0.25:5:5, 0.03:6:4 Emulsion system 3 .

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