A flow photometric analysis flow cell

Abstract

The invention provides a flow photometric analysis flow cell with no bubble interference and high light transmittance. The flow cell comprises a substrate, which is provided with a light incident port and a light exit port. The flow cell is characterized in that a transparent tube passing through the substrate is used as a fluid channel. The transparent tube comprises at least two 90-degree turns in the substrate. The light channel is a section of a straight tube located between the light incident port and the light exit port and turned at both ends. The outer walls of the turning parts at both ends of the light channel are ground into smooth and transparent planes perpendicular to the light channel, forming a plane light incident light transmission window and an exit light transmission window that are integrated with the transparent pipe. The transparent tube is positioned by a groove or a channel prefabricated in the substrate.

Description

Technical Field

[0001] The present invention relates to the field of flow analysis, and more particularly to a photometric detector flow cell for flow photometric analysis in the field of analytical chemistry. Background Art

[0002] Various flow analysis instruments, such as flow injection analyzer, high performance liquid chromatography analyzer, ion chromatography analyzer, capillary electrophoresis analyzer, flow analyzer photoelectric colorimetric detector flow cell, or photoelectric colorimetric detector flow cell of special analysis instruments using flow injection analysis technology, high performance liquid chromatography analysis technology, ion chromatography analysis technology, capillary electrophoresis analysis, flow analysis technology, are measuring components for measuring the analyte in the mobile phase by photometry under flow state.

[0003] The main problem with flow cells for flow colorimetric analysis is that tiny bubbles stay in the light channel. The false absorption caused by the bubble stays makes the test results wrong. The existing flow cell structures are Z-type, U-type, etc. Figure 1 It is a schematic diagram of an existing Z-type flow cell, in which the turning end faces at both ends of the optical channel and the outer wall of the optical channel are all in the shape of large arcs. Most of the incident light entering the flow cell is scattered and refracted, which greatly reduces the light passing through the optical channel of the flow cell, and the corresponding outgoing light is also reduced. The photoelectric signal detected by the photoelectric detector for receiving the outgoing light signal is also weakened, which is not conducive to the use of various flow analysis instruments. Figure 2 This is a schematic diagram of an existing U-shaped flow cell. The flow cell is a black cube with a hole punched through it for the light channel. The two ends of the light channel hole are connected by vertical channels. Transparent glass or quartz glass is pasted on the end faces of the two ends of the light channel hole. Because the light channel of the flow cell has a 90-degree hole connection, a large surface tension is formed, and it is not smooth, bubbles are easy to stay, and the optical signal often has errors.

[0004] In view of the above situation, the present invention provides a flow photometric analysis flow cell, which is characterized in that the outer walls of the corners at both ends of the optical channel are ground into smooth planes, and the curved surfaces of the arc transition at both ends of the optical channel are smaller than the radius of the optical channel, which greatly increases the transmittance of the incident light and the outgoing light of the optical channel, and improves the outgoing light passing through the flow cell. In addition, the inner wall of the entire fluid channel of the flow cell is smooth, and if there are microbubbles in the liquid flow, they will flow out of the optical channel synchronously with the liquid flow, and have the characteristic of no bubble retention. Summary of the invention

[0005] The invention provides a flow photometric analysis circulation pool, which comprises a substrate, wherein the substrate is provided with a light incident port and a light exit port, and is characterized in that a transparent tube passing through the substrate is used as a fluid channel, wherein the transparent tube comprises at least two 90-degree turns in the substrate, and the light channel is a section of a transparent straight tube located between the light incident port and the light exit port and having turns at both ends, wherein the outer walls of the turning parts at both ends of the light channel are ground into smooth and transparent planes perpendicular to the light channel, wherein the planes are perpendicular to the liquid flow channel in the optical path, and become light incident light transmission windows and light exit light transmission windows, wherein the transparent tube is positioned by a groove or a hole prefabricated in the substrate, wherein the outer arcs at both ends formed by the two 90-degree turns in the channel in the transparent tube have an arc radius smaller than a radius of the channel in the transparent tube, and the end points of the arcs at the end faces of the two ends of the light channel do not exceed the center line of the light channel on the light channel surface.

[0006] The flow photometric analysis circulation pool is characterized in that the transparent tube is a transparent glass tube, a transparent quartz glass tube, a transparent ceramic or a transparent plastic tube with basically the same inner diameter, and the tube wall thickness should be such that a transparent light-transmitting window greater than or equal to the inner diameter of the tube can be ground on the tube wall.

[0007] Compared with the prior art, this application has the following advantages:

[0008] The present invention provides a flow photometric analysis flow cell, which is characterized in that the outer walls of the 90-degree turning parts at both ends of the light channel are ground into a plane that is perpendicular to the light channel and smooth and transparent, and the turning arc curved surfaces at both ends of the light channel are smaller than the radius of the light channel, which greatly increases the transmittance of the incident light and the outgoing light of the light channel, and improves the outgoing light passing through the flow cell. In addition, the inner wall of the entire fluid channel of the flow cell is smooth, and the small arc transition structure at the 90-degree corner reduces the surface tension of the corner compared to the flow cell with no arc and right angles, and has the performance of not retaining bubbles, and the outer surface of the light-transmitting window surface is ground into a plane and the inner surface has a structure with a reduced arc curved surface, so that the light refraction is reduced and the light transmission amount is increased. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 It is a schematic diagram of the structure of the existing Z-type circulation pool.

[0010] Figure 2 It is a schematic diagram of the structure of the existing U-shaped circulation pool.

[0011] Figure 3 Schematic diagram of the structure of the Z-type flow photometry flow cell of the present application.

[0012] Figure 4 for Figure 3 Enlarged view of part A.

[0013] Figure 5 This is a schematic diagram of the U-shaped structure of the flow photometry analysis circulation cell in this application.

[0014] Among them, 1 is a connector; 2 is a transparent glass or quartz glass; 3 is a black flow cell seat; 4 is a screw thread; 5 is a transparent pipe; 6 is a flow cell seat; 7 is an exit light port; 8 is a light limiter; 9 is an exit light plane window at the transparent tube light channel; 10 is an incident light port; 11 is an incident light plane window at the transparent tube light channel; 12 is a light channel; 13 is the first 90-degree turning arc; 14 is the second 90-degree turning arc; 121 is the center line of the light channel; 141 is the end point of the arc 14; R is the radius of the arc 14; and D is the diameter of the light channel 12. DETAILED DESCRIPTION

[0015] The flow photometric analysis flow cell of the present application is further described below in conjunction with the accompanying drawings and specific embodiments.

[0016] Embodiment 1:

[0017] like Figure 3 In the Z-shaped flow cell shown, the transparent pipe (5) is fixed on the flow cell base (6), and the turning arcs (13, 14) at both ends of the light channel (12) have small curved surfaces. Figure 4 In the enlarged view of the middle A part, the arc (14) formed by the turning end of the transparent tube and the end face of the light channel has an arc radius (R) less than half of the diameter (D) of the light channel (12), and its arc endpoint (141) does not exceed the center line (121) of the light channel. The outer walls of the turning ends of the light channel (12) are ground into smooth and transparent plane windows (9, 11) perpendicular to the light channel (12). The incident light enters the flow cell through the incident light port (10), passes through the light channel (12) and the light limiter (8), and exits from the output light port (7), and then the photoelectric detector detects its light signal.

[0018] Embodiment 2:

[0019] like Figure 5 The U-shaped flow cell shown in the figure is similarly a transparent pipe (5) fixed on the flow cell base (6), the curved surfaces of the turning arcs (13, 14) at both ends of the light channel (12) are small, and the turning outer walls at both ends of the light channel (12) are ground into smooth and transparent plane windows (9, 11) perpendicular to the light channel (12). Incident light enters the flow cell through the incident light port (10), passes through the light channel (12) and the light limiting plate (8), and comes out from the output light port (7), and then its light signal is detected by the photoelectric detector.

[0020] In summary, the above are only embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A flow photometric analysis flow cell, comprising a substrate, the substrate being provided with a light inlet and a light outlet, characterized in that: A transparent tube passing through a substrate is used as a fluid channel. The transparent tube contains at least two 90-degree turns in the substrate. The light channel is a section of a transparent straight tube located between a light incident port and a light exit port and turned at both ends. The outer walls of the turning parts at both ends of the light channel are ground into smooth and transparent planes perpendicular to the light channel. The planes are perpendicular to the liquid flow channel in the light path and become light incident light transmission windows and light exit light transmission windows. The transparent tube is positioned by a groove or a hole prefabricated in the substrate. The outer arcs at both ends formed by the two 90-degree turns in the channel in the transparent tube have an arc radius smaller than the radius of the channel in the transparent tube. The end points of the arcs at the end faces of the two ends of the light channel do not exceed the center line of the light channel on the light channel surface. The transparent tube is a transparent glass tube, a transparent quartz glass tube, a transparent ceramic or a transparent plastic tube with basically the same inner diameter. The thickness of the tube wall should be such that a transparent light-transmitting window greater than or equal to the inner diameter of the tube can be ground on the tube wall.

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