An experimental chamber device for vascular culture

Abstract

The present invention belongs to the field of biomedical engineering, and provides a vascular culture experimental cavity device that can be repeatedly sterilized at high temperature. By using a vascular connecting tube with adjustable length and a convenient vascular installation device, it provides an experimental cavity device for realizing in vitro simulated culture of blood vessels and can be used for experiments on simulating blood flow stimulation of blood vessels; the vascular assembly is realized through an external fixing device, which enables convenient assembly of blood vessels, is easy to implement, and reduces the risk of contamination during operation; at the same time, the method of pressing realizes the placement of blood vessels into the vascular culture cavity.

Description

Technical Field

[0001] The present invention belongs to the field of biomedical engineering, and relates to an experimental cavity device for vascular culture in experiments, and provides a technical solution for an experimental cavity device for easy operation of in vitro simulated vascular culture. Background Art

[0002] Experimental systems for vascular culture already exist, but the culture cavity is prone to contamination during the vascular assembly process.

[0003] The operation of placing the assembled blood vessels into the blood vessel cavity has poor sealing, resulting in the circulation between the inside and outside of the blood vessels. Summary of the Invention

[0004] The operation of placing the assembled blood vessels into the blood vessel cavity has poor sealing, resulting in the circulation between the inside and outside of the blood vessels.

[0005] To solve the above problems, the following solution is provided: an experimental cavity device for vascular culture; including a vascular culture cavity (1), the vascular culture cavity is composed of a main cavity (1-1) and a cavity cover (1-2), and there is a sealing strip on the cavity cover (1-2) so that the cavity cover is sealed after being fixed to the main cover; an internal circulation joint (1-4), an external vascular circulation joint (1-5) and a reserved joint are fixed outside the main cavity; a pair of vascular joints (2) are fixed inside the main cavity (1-1), and the vascular joint (2) is composed of a vascular sealing and fixing member (2-1), a vascular joint pressing member (2-2), an elastic member (2-3), a sealing ring (2-4) on the vascular sealing and fixing member and a vascular connecting pipe (2-5); when loading the blood vessels, the blood vessels are loaded onto two opposite vascular joints through a vascular assembly device (3), the vascular assembly device consists of a bottom plate (3-1), a magnetic seat (3-2), the magnetic seat is attracted to the bottom plate, and the magnetic seat passes through an adjusting plate (3-3) to connect the vascular joint (2). After loading the blood vessels or simulated blood vessels onto a pair of vascular joints, they are inserted into the vascular culture cavity, and then the vascular assembly device is removed; the cavity cover is covered; each joint on the main body of the vascular culture cavity is used to connect to an external internal circulation pipeline and an external circulation pipeline.

[0006] Furthermore, the materials used for the vascular culture cavity and the vascular joints are biocompatible materials, and the materials are PEEK, titanium alloy or stainless steel; the material of the cavity cover is PC or a high-temperature resistant material such as transparent glass; the elastic member is a material resistant to high temperature and high pressure, for example: silicone.

[0007] Furthermore, the main cavity (1-1) is designed such that the vascular joint (2) realizes the seal between the vascular joint and the main cavity in a press-in manner.

[0008] Further, the blood vessel connector (2) forms a pressing and fixing of the blood vessel connecting tube (2-5) by adjusting the pressing member (2-2) of the blood vessel connector to press the elastic member (2-3). When the blood vessel connecting tube is not pressed and fixed, the blood vessel connecting tube can be loosened by adjusting the blood vessel pressing member, and the length of the connecting tube extending out of the blood vessel connector can be adjusted; the end of the blood vessel connecting tube (2-5) connected to the blood vessel is made with a round opening and a dent for guiding and tying the blood vessel; the wall thickness of the blood vessel connecting tube wall is 0.1 mm - 0.5 mm.

[0009] Further, in the blood vessel assembling device, a magnetic member is fixed in the magnetic base; the bottom plate is made of a magnetically adsorbable material, and the size of the bottom plate can ensure that the assembly does not tip over during blood vessel assembly; the two magnetic bases pass through the adjusting plate and are connected to the blood vessel connector. The magnetic bases can be rotationally adjusted at one end of the adjusting plate and can be rotationally adjusted and the distance from the magnetic base at the other end can be adjusted at the other end to facilitate blood vessel assembly; the maximum distance between the adjustable magnetic bases of the bottom plate is the size of the main cavity into which the blood vessel is inserted. When the blood vessel is assembled, the blood vessel assembling device presses the blood vessel connector with the blood vessel into the main cavity together.

[0010] Further, an internal and external circulation is connected to perform circulating culture of the blood vessel, and it is placed in a CO2 incubator or maintained at a constant temperature and the pH of the culture solution is stabilized in the culture environment for blood vessel culture.

[0011] The beneficial effects of an experimental cavity device for blood vessel culture according to the present invention are as follows:

[0012] 1. The present invention greatly reduces the operation risk of contamination during the blood vessel assembly process.

[0013] 2. The present invention simplifies the sealing operation of putting the assembled blood vessel into the blood vessel cavity.

[0014] 3. The present invention makes it easy to put the blood vessel into the blood vessel culture cavity.

[0015] Therefore, the present invention provides a new device for blood vessel assembly and blood vessel culture cavity for in vitro blood vessel culture. Description of the Drawings

[0016] Figure 1 Example diagram of an experimental cavity device for blood vessel culture

[0017] Figure 2 Culture cavity diagram of an experimental cavity device for blood vessel culture

[0018] Figure 3 Main cavity diagram of the culture cavity of an experimental cavity device for blood vessel culture

[0019] Figure 4 Blood vessel connector diagram of an experimental cavity device for blood vessel culture

[0020] Figure 5 Experimental cavity device for vascular culture - Vascular assembly device diagram

[0021] Figure 6 Experimental cavity device for vascular culture - Vascular assembly diagram

[0022] Figure 7 Experimental cavity device for vascular culture - Vascular assembly diagram

[0023] Figure 8 Experimental cavity device for vascular culture - Regulating plate diagram

[0024] Figure 9 Experimental cavity device for vascular culture - Vascular connector and diagram of vascular assembly into vascular culture cavity

[0025] Figure 10 Experimental cavity device for vascular culture - Vascular culture cavity diagram

[0026] Figure 11 Schematic diagram of a circulatory system using an experimental cavity device for vascular culture

[0027] Legend: 1 Vascular culture cavity, 1 - 1 Vascular culture cavity consists of a main cavity, 1 - 2 cavity cover, 1 - 3 internal vascular circulation connector, 1 - 4 external vascular circulation connector, 2 Vascular connector, 2 - 1 Vascular sealing and fixing part, 2 - 2 Vascular connector pressing part, 2 - 3 Elastic part, 2 - 4 Sealing ring, 2 - 5 Vascular connecting tube; 3 Vascular assembly device, 3 - 1 Vascular assembly device consists of a bottom plate, 3 - 2 Magnetic base, 3 - 3 Regulating plate, 4 Blood vessel; A Guide, B Dimple, C Pipe hole, D Long hole, F Vascular connector fixing clip, P Pressure gauge, L Distance of the vascular connector in the vascular culture cavity Specific implementation method Example

[0028] Experimental chamber device for vascular culture; including a vascular culture chamber (1), which consists of a main chamber (1-1) and a chamber cover (1-2). There is a sealing strip on the chamber cover (1-2) so that the chamber cover is not sealed after being fixed to the main cover; an internal circulation joint (1-4), an external vascular circulation joint (1-5) and a reserved joint are fixed outside the main chamber; a pair of vascular joints (2) are fixed inside the main chamber (1-1). The vascular joint (2) is composed of a vascular sealing and fixing part (2-1), a vascular joint pressing part (2-2), an elastic part (2-3), a sealing ring (2-4) on the vascular sealing and fixing part and a vascular connecting pipe (2-5); when loading the blood vessel, the blood vessel is loaded onto two opposite vascular joints through a vascular assembly device (3). The vascular assembly device consists of a bottom plate (3-1), a magnetic seat (3-2). The magnetic seat is attracted to the bottom plate and passes through an adjusting plate (3-3) to connect to the vascular joint (2). After loading the blood vessel or a simulated blood vessel onto a pair of vascular joints, it is inserted into the vascular culture chamber, and then the vascular assembly device is removed; the chamber cover is covered; each joint on the main body of the vascular culture chamber is used to connect to an external internal circulation pipeline and an external circulation pipeline.

[0029] Furthermore, the materials used for the vascular culture chamber and the vascular joints are biocompatible materials, such as PEEK, titanium alloy or stainless steel; the material of the chamber cover is PC or a high-temperature resistant material such as transparent glass; the elastic part is a material that can withstand high temperature and high pressure, for example, silicone.

[0030] Furthermore, the main chamber (1-1) is designed such that the vascular joint (2) is sealed with the main chamber in a press-in manner.

[0031] Furthermore, the vascular joint (2) forms a pressing and fixing of the vascular connecting pipe (2-5) by adjusting the vascular joint pressing part (2-2) to press the elastic part (2-3). When the vascular connecting pipe is not pressed and fixed, the vascular connecting pipe can be loosened by adjusting the vascular pressing part, and the length of the connecting pipe extending out of the vascular joint can be adjusted; the end of the vascular connecting pipe (2-5) connected to the blood vessel is made with a round mouth and a dent for the guidance and tying of the blood vessel; the wall thickness of the vascular connecting pipe is 0.1mm - 0.5mm.

[0032] Furthermore, in the vascular assembly device, a magnetic part is fixed in the magnetic seat; the bottom plate is a magnetically adsorbable material, and the size of the bottom plate can ensure that the vascular assembly does not tip over during assembly; the two magnetic seats pass through the adjusting plate and are connected to the vascular joints. The magnetic seat can be rotated and adjusted at one end of the adjusting plate, and can be rotated and adjusted and the distance from the magnetic seat at the other end can be adjusted at the other end to facilitate the assembly of the blood vessel; the maximum distance between the magnetically adjustable magnetic seats on the bottom plate is the size for being inserted into the main chamber. After the blood vessel is assembled, the vascular assembly device presses the vascular joint with the blood vessel into the main chamber together.

[0033] Furthermore, connect the internal and external circulation for the circulatory culture of blood vessels, and place it in a CO2 incubator or maintain a constant temperature and keep the pH of the culture medium stable in the culture environment.

Claims

1. An experimental cavity device for vascular culture, characterized in that It includes a vascular culture chamber (1), which is composed of a main chamber (1-1) and a chamber cover (1-2). There is a sealing strip on the chamber cover (1-2) so that the chamber cover (1-2) is sealed after being fixed to the main chamber (1-1); an internal circulation joint (1-4), an external vascular circulation joint (1-5) and a reserved joint are fixed outside the main chamber (1-1); a pair of vascular joints (2) are fixed inside the main chamber (1-1), and the vascular joint (2) is composed of a vascular sealing and fixing part (2-1), a vascular joint pressing part (2-2), an elastic part (2-3), a sealing ring (2-4) on the vascular sealing and fixing part and a vascular connecting pipe (2-5); when loading the blood vessel, the blood vessel is loaded onto two opposite vascular joints (2) through a vascular assembly device (3), and the vascular assembly device is composed of a bottom plate (3-1) and a magnetic seat (3-2). The magnetic seat (3-2) is attracted to the bottom plate, and the magnetic seat (3-2) passes through an adjusting plate (3-3) to connect the vascular joint (2). After loading a blood vessel or a simulated blood vessel onto a pair of vascular joints (2), the vascular assembly device (3) is removed after inserting it into the vascular culture chamber (1); the chamber cover (1-2) is covered; each joint on the main chamber (1-1) of the vascular culture chamber is used to connect to an external internal circulation pipeline and an external circulation pipeline, and the materials used for the vascular culture chamber (1) and the vascular joint (2) are biocompatible materials; the vascular joint (2) forms a pressing and fixing of the vascular connecting pipe (2-5) by pressing the vascular joint pressing part (2-2) to compress the elastic part (2-3). When the vascular connecting pipe (2-5) is not pressed and fixed, the vascular joint pressing part (2-2) can be adjusted to loosen the vascular connecting pipe (2-5) and adjust the length of the vascular connecting pipe (2-5) extending in the vascular joint (2); the vascular connecting pipe (2-5) and the vascular connection end are made with a round mouth and a dent for guiding and tying the blood vessel; the wall thickness of the vascular connecting pipe (2-5) is 0.1 mm - 0.5 mm.

2. The experimental cavity device for blood vessel culture according to claim 1, characterized in that The biocompatible material is PEEK, titanium alloy or stainless steel; the material of the chamber cover (1-2) is PC or a high-temperature resistant material such as transparent glass; the elastic part (2-3) is a material resistant to high temperature and high pressure.

3. The experimental cavity device for blood vessel culture according to claim 1, wherein The vascular joint (2) realizes the seal between the vascular joint (2) and the main chamber (1-1) in a press-in manner.

4. According to the experimental cavity device for blood vessel culture described in claim 1, in the blood vessel assembly device (3), a magnetic member is fixed in the magnetic base (3-2); the bottom plate (3-1) is made of a magnetically adsorbable material, and the size of the bottom plate (3-1) can ensure that the blood vessel does not tip over during assembly; the two magnetic bases (3-2) pass through the adjusting plate (3-3) and are connected to the blood vessel connector (2). The magnetic base (3-2) can be rotationally adjusted at one end of the adjusting plate (3-3), and at the other end, it can be rotationally adjusted and the distance from the magnetic base (3-2) at the other end can be adjusted to facilitate the assembly of the blood vessel; the maximum distance between the adjustable magnetic bases (3-2) of the bottom plate (3-1) is the size for being inserted into the main cavity (1-1). After the blood vessel is assembled, the blood vessel assembly device (3) presses the blood vessel connector (2) with the blood vessel into the main cavity (1-1) together.

Images