Genetic testing equipment

Abstract

The present invention discloses a gene detection device, wherein a purification and heating device is installed at the first end of an operating platform, a metal bath module and an optical detector are installed at the second end of the operating platform, the optical detector is installed at the upper part of the metal bath module, reagents, samples, a gun tip processing module, and a heat sealer are installed between the first end and the second end of the operating platform, a liquid transfer assembly is installed at the upper part of the reagents, samples, gun tip processing module, and the purification and heating device, and a PCR plate transport module and a film sealing plate transport module are installed on the operating platform in parallel with each other; the PCR plate transport module is used for the PCR plate transmission from the reagent, sample, and gun tip processing module to the heat sealer; and the film sealing plate transport module is used for the PCR plate transmission from the heat sealer to the metal bath module. In this application, the automatic detection of genes is realized through the combined action of the purification and heating device, the liquid transfer assembly, the operating platform, the metal bath module, the optical detector, the PCR plate transport module, the film sealing plate transport module, and the heat sealer, thereby increasing the detection efficiency.

Description

Technical Field

[0001] The present invention belongs to the technical field of gene detection, and in particular relates to a gene detection device. Background Art

[0002] Genetic testing refers to the analysis of the base sequence of a specific DNA fragment, that is, the arrangement of adenine, thymine, cytosine and guanine; genes are the basic units of heredity, DNA or RNA sequences that carry genetic information, which are replicated to pass on genetic information to the next generation, guide the synthesis of proteins to express the genetic information they carry, and thus control the expression of the characteristics of biological individuals. Genetic testing is a technology that detects DNA through blood, other body fluids, or cells. It is a method of taking peripheral venous blood or other tissue cells of the subject, amplifying their genetic information, and then using specific equipment to detect the DNA molecular information in the cells of the subject, analyzing the gene types and gene defects it contains and whether its expression function is normal, so that people can understand their own genetic information, clarify the cause of the disease or predict the risk of a certain disease. Genetic testing can diagnose diseases and can also be used to predict disease risks.

[0003] The existing genetic testing equipment is unable to automate the entire testing process, resulting in low genetic testing efficiency.

[0004] Therefore, it is urgent to develop a gene detection device to solve the above problems. Summary of the invention

[0005] In order to solve the problems raised in the above background technology, the present invention provides a gene detection device.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] Genetic testing equipment, including:

[0008] Purification and heating device for nucleic acid purification and extraction;

[0009] Pipetting components for reagent and sample transfer;

[0010] An operating platform for installing reagents, samples, and gun tip processing modules;

[0011] Metal bath module for PCR amplification reaction;

[0012] Optical detectors for genetic testing;

[0013] PCR plate transport module;

[0014] Sealing plate transport module;

[0015] Heat sealer for PCR plate sealing;

[0016] Among them, the purification and heating device is installed at the first end of the operating platform, the metal bath module and the optical detector are installed at the second end of the operating platform, the optical detector is installed on the upper part of the metal bath module, the reagents, samples, gun tip processing module, and heat sealer are installed between the first end and the second end of the operating platform, the reagents, samples, and gun tip processing modules are installed close to the first end of the operating platform, the heat sealer is installed close to the second end of the operating platform, the pipetting assembly is installed on the reagents, samples, gun tip processing modules and the purification and heating device, the PCR plate transport module and the film sealing plate transport module are installed parallel to each other on the operating platform; the PCR plate transport module is used for transferring the PCR plate from the reagent, sample, and gun tip processing module to the heat sealer; the film sealing plate transport module is used for transferring the PCR plate from the heat sealer to the metal bath module.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] In the present application, automatic detection of genes is achieved and detection efficiency is increased through the combined effects of a purification heating device, a pipetting component, an operating platform, a metal bath module, an optical detector, a PCR plate transport module, a sealing plate transport module, and a heat sealer. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the first-view stereoscopic structure in this application;

[0020] Figure 2 This is a schematic diagram of the second viewing angle stereoscopic structure in this application;

[0021] Figure 3 This is a schematic diagram of the third perspective stereoscopic structure in this application;

[0022] Figure 4 It is a schematic diagram of the internal structure in this application;

[0023] Figure 5 This is a schematic diagram of the structure of the operating platform in this application;

[0024] Figure 6 A top view of the operating platform in this application;

[0025] Figure 7 This is a schematic diagram of the structure of the purification and heating device in this application. Figure 1 ;

[0026] Figure 8 The structure of the purification heating device in this application is shown in FIG. Figure 2 ;

[0027] Fig. 9 This is a schematic diagram of the structure of the purification and heating device in this application. Figure 3 ;

[0028] Fig.10 It is a schematic diagram of the structure of the sample box in this application;

[0029] Fig.11 It is a schematic diagram of the three-dimensional structure of the liquid transfer component in this application;

[0030] Fig.12 It is a schematic diagram of the structure of the X-axis moving component and the Y-axis moving component in this application;

[0031] Fig.13 It is a schematic diagram of the structures of the Z-direction moving component A, the Z-direction moving component B, the Z-direction moving component C, and the Z-direction moving component D in this application;

[0032] Fig.14 This is a detailed structural diagram of the Z-axis moving component B in this application;

[0033] Fig.15 It is a schematic diagram of the structure of the Z-direction moving component D in this application;

[0034] Fig.16 This is a schematic diagram of the structure of the gun head and the mounting box in this application;

[0035] Fig.17 This is a schematic diagram of the connection structure between the gun head and the connector in this application;

[0036] Fig.18 It is a schematic diagram of the structure of the connector in this application;

[0037] Fig.19 This is a schematic diagram of the installation structure of the connecting plate in this application;

[0038] Fig. 20 This is a schematic diagram of the structure of the clamping block and the spring in this application;

[0039] Fig.21 It is a schematic diagram of the structure of the heat sealing machine in this application;

[0040] Fig. 22 It is a schematic diagram of the structure of the heat sealing plate in this application;

[0041] Fig.23 This is a schematic diagram of the structure of the lifting device A in this application. Figure 1 ;

[0042] Fig.24 This is a schematic diagram of the structure of the lifting device A in this application. Figure 2 ;

[0043] Fig.25 It is a schematic diagram of the structure of the blocking device and the PCR plate recovery component in this application;

[0044] Fig.26It is a schematic diagram of the structure of the blocking device in this application;

[0045] Fig. 27 The structure of the heat cover assembly in this application is shown in FIG. Figure 1 ;

[0046] Fig.28 The structure of the thermal cover assembly in this application is shown in FIG. Figure 2 ;

[0047] Fig.29 It is a schematic diagram of the structure of the thermal cover in this application;

[0048] Fig.30 It is a schematic diagram of the structure of the metal bath module in this application;

[0049] Fig.31 It is a schematic diagram of the structure of the metal bath module and the optical detector in this application;

[0050] Fig.32 A schematic diagram of the position of the optical filtering device in this application;

[0051] Fig.33 It is a schematic diagram of the structure of the moving parts in this application;

[0052] Fig.34 The structure of the optical filter device in this application is shown in FIG. Figure 1 ;

[0053] Fig.35 The structure of the optical filter device in this application is shown in FIG. Figure 2 ;

[0054] Fig.36 The structure of the optical filter device in this application is shown in FIG. Figure 3 ;

[0055] Fig.37 Schematic diagram of the optical path of the optical filtering device in this application.

[0056] In the attached figure:

[0057] 1- Shell,

[0058] 2-Storage window,

[0059] 3- Observation window,

[0060] 4-Liquid circuit unit,

[0061] 5- Power supply unit,

[0062] 6-Recycling port A,

[0063] 7-Recycling port B,

[0064] 8-pipette assembly, 81-X-direction moving assembly, 811-X-direction moving guide rail A, 812-X-direction moving guide rail B, 813-X-direction electric cylinder, 814-X-direction chain; 82-Y-direction moving assembly, 821-carrying plate, 8210-sub-rail, 822-support A, 823-support B, 824-screw A, 825-Y-direction moving part, 826-X-direction motor, 827-pulley A, 828-pulley B, 829-screw sleeve A; 83-Z-direction moving assembly A, 831-frame A, 8310-mother rail, 832-cross plate A, 833-Z-direction motor A, 834-screw B, 835-screw sleeve B, 836-frame B; 84-Z-direction moving assembly B, 841-Z-direction motor B, 842-cross plate B, 843-guide rod, 844-screw C, 845-frame C, 846-screw sleeve C, 847-steel needle group A; 85-Z-direction moving component C, 851-connecting piece, 852-housing, 853-Z-direction motor C, 854-steel needle group B, 855-mounting plate, 856-high-frequency valve, 857-pipeline winder, 858-screw D, 859-screw sleeve D; 86-Z-direction moving component D, 861-gun head, 862-mounting box, 8621-connecting rod, 8622-block, 8623-spring A, 863-piston chamber assembly, 864-piston assembly, 8641-connecting plate, 865-screw sleeve E, 866-screw E, 867-frame D, 868-Z-direction motor D, 869-connector, 8691-conical nozzle; 87-barcode scanner,

[0065] 9-operating platform, 91-gun tip storage module, 92-sample cooling module, 93-reagent cooling module A, 94-needle cleaning module A, 95-PCR plate, 96-needle cleaning module B, 97-reagent cooling module B, 98-positive reference cooling module, 99-gun tip recovery assembly, 910-opening A, 911-opening B, 912-opening C,

[0066] 10-metal bath module, 101-heat cover assembly, 1011-limiting block, 1012-through hole, 1013-metal heat cover, 1014-mounting bracket A, 1015-positioning hole, 1016-elastic part, 1018-positioning pin, 1019-moving part,

[0067] 11-optical detector, 111-light filtering device, 1111-first filter, 1112-mounting bracket B, 1113-second filter, 1114-light receiving box, 1115-two-way mirror, 1116-light hole, 1117-light, 1118-total reflection mirror,

[0068] 12-purification heating device, 1201-sample box, 1202-heating groove, 1203-first heating chamber, 1204-second heating chamber, 1205-metal block, 1206-boss, 1207-placing table, 1208-through groove, 1209-heating plate, 1210-heating base,

[0069] 13-tray module, 131-tray A, 132-linear motor assembly A,

[0070] 14-PCR plate transport module, 141-linear motor assembly B, 142-transport rack A,

[0071] 15-film sealing plate transport module, 151-linear motor assembly C, 152-transport rack B,

[0072] 16-heat sealer, 1601-tray B, 1602-slider, 1603-film sealing plate, 1604-heating plate, 1605-spring B, 1606-fixed plate, 1607-linear guide, 1608-cylinder, 1609-photoelectric sensor A, 1610-mounting base, 1611-synchronous belt transmission mechanism, 1612-positioning port, 1613-lifting device A,

[0073] 17-Partition,

[0074] 18-blocking device, 181-blocking motor, 182-connecting block, 183-rotating wheel, 184-connecting long rod, 185-sliding groove, 186-baffle, 187-sliding groove module, 188-sliding rail module,

[0075] 19-PCR plate recovery assembly, 191-photoelectric sensor B, 192-pick. DETAILED DESCRIPTION

[0076] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0077] The present invention provides the following technical solutions:

[0078] like Figure 4 , 5 As shown, the genetic testing equipment includes:

[0079] A purification and heating device 12 for nucleic acid purification and extraction;

[0080] A pipetting component 8 for transferring reagents and samples;

[0081] An operating platform 9 for installing reagents, samples, and a gun tip processing module;

[0082] A metal bath module 10 for PCR amplification reaction;

[0083] Optical detector for genetic testing 11;

[0084] PCR plate transport module 14;

[0085] Sealing plate conveying module 15;

[0086] Heat sealer 16 for PCR plate sealing;

[0087] Among them, the purification and heating device 12 is installed at the first end of the operating platform 9, the metal bath module 10 and the optical detector 11 are installed at the second end of the operating platform 9, the optical detector 11 is installed on the upper part of the metal bath module 10, the reagents, samples, gun tip processing module, and the heat sealer 16 are installed between the first end and the second end of the operating platform 9, the reagents, samples, and gun tip processing modules are installed close to the first end of the operating platform 9, the heat sealer 16 is installed close to the second end of the operating platform 9, the pipetting assembly 8 is installed on the reagents, samples, gun tip processing modules and the purification and heating device 12, the PCR plate transport module 14 and the sealing plate transport module 15 are installed parallel to each other on the operating platform 9; the PCR plate transport module 14 is used to transfer the PCR plate 95 from the reagent, sample, and gun tip processing module to the heat sealer; the sealing plate transport module 15 is used to transfer the PCR plate 95 from the heat sealer 16 to the metal bath module 10.

[0088] In this embodiment, the PCR plate transport module 14 includes a linear motor assembly B141 and a transport rack A142; the moving end of the linear motor assembly B141 is connected to the transport rack A142; the sealing plate transport module 15 includes a linear motor assembly C151 and a transport rack B152; the moving end of the linear motor assembly C151 is connected to the transport rack B152; the moving range of the PCR plate transport module is generally from the reagent, sample, and gun tip processing module to the heat sealer, and the moving range of the sealing plate transport module is from the heat sealer to the PCR plate recovery assembly 19.

[0089] When the present embodiment is working, the working process is as follows: the sample nucleic acid is purified and amplified by the purification and heating device; the sample is transferred to the PCR plate by the pipetting component 8; various reagents are added and various sample operations are performed by the pipetting component 8; the PCR plate is transferred to the heat sealer by the PCR plate transport module to seal the PCR plate, and after the PCR plate is sealed, the PCR plate is transported to the metal bath module 10 through the partition 17 by the sealing plate transport module to perform PCR amplification reaction, and optical detection is performed by the optical detector 11 at the same time, and the PCR plate after the detection is completed is transported forward by the sealing plate transport module and recovered by the PCR plate recovery component 19.

[0090] like Fig.25 As shown, the PCR plate recovery component 19 includes a photoelectric sensor B191 and a paddle 192. When the PCR plate moves to the paddle 192, the paddle 192 contacts the PCR plate, and the paddle 192 pushes the PCR plate to tilt and fall into the opening C912, and then is discharged from the recovery port B.

[0091] like Figure 5 , 6 As shown, the genetic testing equipment also includes: a tray module, the tray module 13 includes a tray A131 and a linear motor assembly A132, the linear motor assembly A132 is installed on the operating platform 9, and is located between the reagents, samples, gun tip processing module and the purification and heating device, the tray A131 is connected to the moving part of the linear motor assembly A132, the tray A131 is synchronized with the lateral movement of the gun tip 861 on the pipetting assembly 8, when the gun tip 861 on the pipetting assembly 8 passes over the tray module 13, the tray A131 is always placed under the gun tip 861.

[0092] In some embodiments, a microprocessor is provided to coordinately control the entire device, and a control signal output terminal of the microprocessor is connected to a control signal input terminal of the moving component and a signal input terminal of the tray module.

[0093] like Figure 11-15 As shown, the pipetting assembly 8 includes:

[0094] An X-direction moving assembly 81 for moving the pipetting action part along the X-direction; the X-direction moving assembly 81 comprises an X-direction moving guide rail A811, an X-direction moving guide rail B812, and an X-direction electric cylinder 813; the X-direction moving guide rail A811 and the X-direction moving guide rail B812 are parallel; the Y-direction moving assembly 82 is fixedly mounted on the sliding part of the X-direction electric cylinder 813;

[0095] A Y-direction moving assembly 82 for moving the liquid transfer action part along the Y-direction; the Y-direction moving assembly 82 comprises a bearing plate 821, a support A822, a support B823, a screw A824, a Y-direction moving member 825, an X-direction motor 826, a pulley A827, a pulley B828, and a screw sleeve A829; the support A822 and the support B823 are respectively mounted at two ends of the bearing plate 821, and two ends of the screw A824 are rotatably mounted on the support A822 and the support B823; the screw sleeve A829 is mounted on the Y-direction moving member 825, the screw sleeve A829 is threadedly mounted with the screw A824, the pulley B828 is fixedly connected to one end of the screw A824, the X-direction motor 826 is mounted on the bearing plate 821, the rotating shaft of the bearing plate 821 is fixedly connected to the pulley A827, and the pulley A827 is connected to the pulley B828 through a belt drive;

[0096] A Z-direction moving assembly for moving the pipetting action part along the Z direction; the Z-direction moving assembly includes a Z-direction moving assembly B84 for moving the steel needle assembly A847 along the Z direction, a Z-direction moving assembly C85 for moving the steel needle assembly B854 along the Z direction, a Z-direction moving assembly D86 for moving the gun head 861 along the Z direction, and a Z-direction moving assembly A83 for moving the Z-direction moving assembly B84, the Z-direction moving assembly C85 and the Z-direction moving assembly D86 along the Z direction;

[0097] Among them, the Y-axis moving component 82 is installed on the moving part of the X-axis moving component 81, the Z-axis moving component A83 is installed on the moving part of the Y-axis moving component 82, and the Z-axis moving component B84, the Z-axis moving component C85 and the Z-axis moving component D86 are all installed on the moving part of the Z-axis moving component A83.

[0098] like Figure 11-20 As shown, the Z-direction moving assembly A83 includes a frame A831, a horizontal plate A832, a Z-direction motor A833, a screw rod B834, a screw rod sleeve B835, and a frame B826; the frame A831 is fixedly mounted on the moving part of the Y-direction moving assembly 82, the horizontal plate A832 is fixedly mounted in the frame A831, the Z-direction motor A833 is invertedly mounted on the horizontal plate A832, the rotating shaft of the Z-direction motor A833 passes through the horizontal plate A832 and is fixedly connected to one end of the screw rod B834, the screw rod sleeve B835 is threadedly mounted with the screw rod B834, and the screw rod sleeve B835 is fixedly mounted on the frame B826; the frame B826 is placed in the frame A831 and can be moved up and down, and is located below the horizontal plate A832;

[0099] The Z-axis moving assembly B84 includes a Z-axis motor B841, a transverse plate B842, a guide rod 843, a screw C844, a frame C845, a screw sleeve C846, and a steel needle group A847. The transverse plate B842 is fixedly installed in the frame B826, the Z-axis motor B841 is invertedly installed on the transverse plate B842, the rotating shaft of the Z-axis motor B841 passes through the transverse plate B842 and is fixedly connected to one end of the screw C844, the screw sleeve C846 is threadedly installed with the screw C844, and the screw sleeve C846 is fixedly installed on the frame C845; the frame C845 can be moved up and down and is placed in the frame B826 and is located below the transverse plate B842, the steel needle group A847 is installed on the frame C845, and the steel needle group A847 is connected to the external liquid circuit;

[0100] The Z-direction moving assembly C85 comprises a connecting piece 851, a housing 852, a Z-direction motor C853, a steel needle group B854, a mounting plate 855, a high-frequency valve 856, a pipeline winder 857, a screw rod D858, and a screw rod sleeve D859. The connecting piece 851 is connected to the frame B826, the Z-direction motor C853 is mounted on the connecting piece 851, the steel needle group B854, the high-frequency valve 856, the pipeline winder 857, and the screw rod sleeve D859 are all mounted on the mounting plate 855, the Z-direction motor C853 is mounted inverted and vertically, the rotating shaft of the Z-direction motor C853 is fixedly connected to one end of the screw rod D858, the screw rod sleeve D859 is threadedly mounted to the screw rod D858, the external liquid circuit is connected to one end of the high-frequency valve 856, and the other end of the high-frequency valve 856 is connected to the steel needle group B854 through the pipeline winder 857;

[0101] The Z-direction moving assembly D86 includes a gun head 861, a mounting box 862, a connecting rod 8621, a block 8622, a spring A8623, a piston chamber assembly 863, a piston assembly 864, a connecting plate 8641, a screw sleeve E865, a screw E866, a frame D867, a Z-direction motor D868, a connector 869, and a tapered nozzle 8691; the frame D867 is fixedly connected to the frame B826, the Z-direction motor D868 is invertedly installed on the upper end of the frame D867, and the rotating shaft of the Z-direction motor D868 passes through the upper end of the frame D867 and is connected to the Z-direction motor D868. One end of the screw rod E866 is fixedly connected, the screw rod sleeve E865 is installed in cooperation with the screw rod E866 thread, the screw rod sleeve E865 is fixedly installed on the upper end of the housing 852 of the piston assembly 864, and the piston assembly 864 is installed in the frame D867 so as to be movable up and down; the piston chamber assembly 863 is fixedly installed in the frame D867, the piston chamber assembly 863 is placed below the piston assembly 864, and the piston chamber assembly 863 is installed in cooperation with the piston assembly 864; the gun head 861 is placed below the piston chamber assembly 863, and is connected and cooperated with the piston chamber assembly 863.

[0102] The Z-axis moving component D86 also includes an installation box 862, a connecting rod 8621, a block 8622, a spring A8623, a connecting plate 8641, and a connector 869; the connecting plate 8641 is arranged at the lower end of the piston assembly 864, and a stepped through hole is vertically opened on the connecting plate 8641, and the upper diameter of the stepped through hole is larger than the lower diameter. The upper end of the connecting rod 8621 is connected to the block 8622, and the lower end of the connecting rod 8621 is fixedly connected to the installation box 862, and the diameter of the block 8622 is slightly smaller than the stepped through hole. The spring A8623 is mounted on the connecting rod 8621, and the spring A8623 is installed Installed between the step of the stepped through hole and the block 8622; a plurality of through holes A are provided on the installation box 862, and a plurality of connectors 869 can be slidably installed in the through holes A. A tapered nozzle 8691 is provided at the lower end of the connector 869, and the tapered nozzle 8691 is inserted into the upper end of the gun head 861 for installation; when the piston assembly 864 is pressed down to the bottom, the piston assembly 864 squeezes the block 8622 downward, the spring A8623 is compressed, the block 8622 slides into the upper hole of the stepped through hole, the connecting rod 8621 pushes the installation box 862 to move downward, and the installation box 862 pushes the gun head 861 out of the tapered nozzle 8691.

[0103] In some embodiments, there are twelve gun tips 861 , and the number of gun tips 861 can be adjusted according to the number of rows and columns of the sample box.

[0104] In some embodiments, the number of steel needles in the steel needle group A847 is twelve, and the number of steel needles in the steel needle group A847 can be adjusted according to the number of rows and columns of the sample box.

[0105] In some embodiments, the number of steel needles in the steel needle group B854 is generally four, in order to adapt to the shape of the reagent tank. Of course, in order to adapt to reagent tanks of different shapes, the number of steel needles in the steel needle group B854 can be selected arbitrarily.

[0106] In some embodiments, the gun head 861, the steel needle group A847, and the steel needle group B854 are all arranged vertically downward; and the arrangement directions of the steel needles of the gun head 861 and the steel needle group A847 are parallel, and the arrangement directions of the steel needle group B854 and the steel needle group A847 are vertical.

[0107] In this embodiment, the movement of the Y-direction moving component 82 and the Z-direction moving component along the X-direction is realized through the operation of the X-direction electric cylinder 813, which is a prior art and will not be elaborated here.

[0108] When this embodiment is working, the X-direction motor 826 provides the main power, and the output power of the X-direction motor 826 is transmitted to the screw rod A824 through the pulley A827. The screw rod A824 rotates, and the Y-direction moving part 825 starts to move, realizing the movement of the Z-direction moving component along the Y direction.

[0109] In this embodiment, a through slot along the Y direction is provided on the bearing plate 821, and the Y-direction moving member 825 passes through the through slot and cooperates with the screw A824 through the screw sleeve A829. In order to fit the through slot, the Y-direction moving member 825 is formed into a structure with a narrow upper end and a wide lower end. In order to fit the installation of the Z-direction motor A833, the Y-direction moving member 825 has an upward notch at its lower end for placing the Z-direction motor A833, thereby saving vertical space.

[0110] In this embodiment, the pipetting component 8 also includes an X-axis drag chain 814 and a Y-axis drag chain. The two ends of the X-axis drag chain 814 are respectively installed on the X-axis moving guide rail A811 and the supporting plate 821; the two ends of the Y-axis drag chain are respectively installed on a fixed peripheral device and the supporting plate 821; the setting of the drag chain belongs to the prior art, and the drag chain is used to restrain cables and pipelines, and plays the role of pulling and protecting cables and pipelines.

[0111] In this embodiment, a mother rail 8310 is horizontally arranged on the frame A831, and a sub-rail 8210 is horizontally arranged on the bearing plate 821. The mother rail 8310 and the sub-rail 8210 are slidably matched and are used to limit the vertical displacement of the frame A831. In other cases, there are two mother rails 8310, which are respectively placed on both sides of the frame A831, and two sub-rails 8210, which are respectively placed on both sides of the bearing plate 821. The purpose of such a setting is to maintain balance and facilitate relative sliding.

[0112] In some embodiments, frame B826 and frame D867 are integrally formed, and protrusions are provided on both sides of the integrally formed frame. Correspondingly, sliding grooves are vertically opened on both sides of frame A831. The width of the sliding groove is slightly larger than the width of the protrusion. The protrusion slides up and down along the sliding groove, and frame A831 limits the horizontal movement of frame B826 and frame D867.

[0113] In some embodiments, the screw rod C844 can pass through the upper end of the frame C845 and enter the interior of the frame C845, and sufficient space for the screw rod C844 to move is reserved inside the frame C845.

[0114] In some embodiments, the piston assembly 864 includes twelve pistons arranged side by side, and the piston chamber assembly 863 includes twelve piston chambers arranged side by side. The pistons are installed in coordination with the piston chambers. The lower end of the screw rod E866 is connected to a push plate, and the push plate is connected to the upper parts of the twelve pistons. Rotating the screw rod E866 in different directions will cause the piston to move forward or backward in the piston chamber to complete the suction of the reagent.

[0115] In this embodiment, the diameter of the through hole A on the installation box 862 is smaller than the outer diameter of the gun head 861, so that the gun head 861 cannot enter the installation box 862. Therefore, the gun head 861 can be pushed out of the connector 869 when the installation box 862 moves downward.

[0116] In this embodiment, when it is necessary to switch to another gun head 861, it is necessary to first let the Z-axis motor D868 work in reverse, and the screw E866 rotates in the opposite direction. With the cooperation of the screw sleeve A829E, the piston assembly 864 moves upward, and the spring A8623 rebounds to pull up the connecting rod 8621, and then pull up the installation box 862, so that the tapered nozzle 8691 of the connector 869 is exposed. At this time, the tapered nozzle 8691 can be inserted into the new gun head 861.

[0117] In some embodiments, the connecting member 851 is vertically installed on the side wall of the frame B826, and a raised strip-shaped slider is vertically arranged on one side surface of the connecting member 851, and a slide groove is vertically arranged on one side of the mounting plate 855. The slider and the slide groove are slidably matched and limit the horizontal movement of the mounting plate 855.

[0118] In some embodiments, the Z-axis moving component B84 also includes a guide rod 843, the lower end of which is fixedly mounted on the upper end of the frame C845, and a guide groove is provided on the transverse plate B842, and the guide rod 843 passes through the guide groove on the transverse plate B842 for guiding engagement.

[0119] In some embodiments, in some embodiments, there are two guide rods 843, which are symmetrically distributed on both sides of the screw rod C844, and the lower ends of the two guide rods 843 are fixedly mounted on the upper end of the frame C845, and the upper ends of the two guide rods 843 are installed through the guide groove on the cross plate B842, and the guide rods 843 and the cross plate B842 are slidably matched.

[0120] In some embodiments, the pipetting component 8 includes a barcode scanner 87, which is installed on a frame formed integrally of frame B826 and frame D867 and is used to scan and confirm the barcode on the sample box; only the sample box that has passed the scan will prompt the pipetting component 8 to work.

[0121] In this embodiment, the pipetting action in three directions is realized through the X-direction moving component 81, the Y-direction moving component 82, and the Z-direction moving component; the primary regulation of the steel needle group A847, the steel needle group B854, and the gun tip 861 in the Z direction is realized through the Z-direction moving component A83; the secondary regulation of the steel needle group A847 in the Z direction is realized through the Z-direction moving component B84, and the secondary regulation of the steel needle group B854 in the Z direction is realized through the Z-direction moving component C85; the secondary regulation of the gun tip 861 in the Z direction is realized through the Z-direction moving component D86, and the travel regulation of the steel needle group A847, the steel needle group B854, and the gun tip 861 in the Z direction is increased; in addition, the steel needle group A847, the steel needle group B854, and the gun tip 861 all have different quantities, which are suitable for transferring reagents in different reagent tanks and sample boxes.

[0122] like Figure 5 As shown, the reagent, sample and gun tip processing module include a gun tip storage module 91 for storing gun tips 861, a sample cooling module 92, a reagent cooling module A93, a reagent cooling module B97, a needle cleaning module A94, a needle cleaning module B96, a positive reference cooling module 98 and a gun tip recovery assembly 99; the gun tip storage module 91, the sample cooling module 92, the reagent cooling module A93, the needle cleaning module A94, the needle cleaning module B96, the reagent cooling module B97, the positive reference cooling module 98 and the gun tip recovery assembly 99 are all installed on the operating platform 9; the needle cleaning module A94 is used for cleaning the steel needle group A847, and the needle cleaning module B96 is used for cleaning the steel needle group B854.

[0123] In this embodiment, because it is necessary to adapt the arrangement of the gun tip 861 and the steel needle, and the pipetting component 8 cannot rotate, the installation position of the gun tip storage module 91, the sample cooling module 92, the reagent cooling module A93, the needle cleaning module A94, the needle cleaning module B96, the reagent cooling module B97, the positive reference cooling module 98, and the gun tip recovery component 99 needs to be determined according to the arrangement position of the gun tip 861 and the steel needle, so as to ensure that the gun tip 861 and the steel needle can act on the corresponding processing module during operation.

[0124] like Figure 7-10 As shown, in some embodiments,

[0125] The purification and heating device 12 is used to heat the gene sample in the sample box 1201, and includes:

[0126] At least one first heating element; the heating end of the first heating element acts on the genetic samples with the same heating requirements in the sample box 1201;

[0127] The first heating chamber 1203; the first heating element is installed in the first heating chamber 1203;

[0128] The second heating element; the heating end of the second heating element acts on all the gene samples in the sample box 1201, and the number of gene samples acted on by the heating end of the first heating element is less than the number of gene samples acted on by the heating end of the second heating element;

[0129] The second heating chamber 1204 ; the second heating element is installed in the second heating chamber 1204 .

[0130] When all the gene samples in the sample box 1201 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1201. When only a part of the gene samples in the sample box 1201 needs to be heated, without taking out the samples that need to be heated separately, the heating mode of the first heating element is selected to heat the gene samples that need to be heated in the sample box 1201, and other gene samples that do not need to be heated are not affected.

[0131] In some embodiments, the second heating element is a heating base 1210 , a heating plate 1209 is installed inside the heating base 1210 , and the upper surface of the heating base 1210 contacts the bottom of the sample box 1201 to heat all the gene samples in the sample box 1201 .

[0132] When all the gene samples in the sample box 1201 need to be heated, the bottom of the sample box 1201 is placed on the upper surface of the heating base 1210, and the upper surface of the heating base 1210 is in contact with the bottom of the sample box 1201 to heat all the gene samples in the sample box 1201; when only a part of the gene samples in the sample box 1201 need to be heated, without taking out the samples that need to be heated separately, the heating mode of the first heating element is selected to heat the gene samples that need to be heated in the sample box 1201, and other gene samples that do not need to be heated are not affected.

[0133] In some embodiments: a plurality of heating grooves 1202 are disposed on the upper surface of the heating base 1210, and the bottom of each sample placement position of the sample box 1201 bulges outward to form a boss 1206, and a heating groove 1202 contacts the boss 1206 at the bottom of a sample placement position to heat the gene sample in the sample placement position.

[0134] When all gene samples in the sample box 1201 need to be heated, the boss 1206 at the bottom of each sample position of the sample box 1201 contacts the heating groove 1202 on the heating base 1210, thereby increasing the contact area between the bottom of the sample position and the heating base 1210 and accelerating the heating efficiency of the heating base 1210 for the gene samples.

[0135] In some embodiments, the heating groove 1202 on the heating base 1210 and the boss 1206 at the bottom of the sample placement position are both hemispherical.

[0136] By making the heating groove 1202 on the heating base 1210 and the boss 1206 at the bottom of the sample placement position both hemispherical, the contact area between the bottom of the sample placement position and the heating base 1210 is further increased, and the heating efficiency of the heating base 1210 on the gene sample is further accelerated.

[0137] In some embodiments, the second heating element is a liquid and a heating plate 1209. The liquid is contained in the second heating chamber 1204. The heating plate 1209 is used to heat the liquid. When all genetic samples are heated, the sample box 1201 is located in the second heating chamber 1204. The liquid is wrapped around the side and bottom of the sample box 1201. The highest height of the liquid is slightly lower than the side wall height of the sample box 1201. The liquid is water.

[0138] When all the gene samples in the sample box 1201 need to be heated, the sample box 1201 is placed in the liquid in the second heating chamber 1204, so that the liquid wraps the side and bottom of the sample box 1201, and the highest height of the liquid is slightly lower than the side wall height of the sample box 1201. The heating plate 1209 heats the liquid to increase the temperature of the liquid, so that the gene samples in the sample box 1201 are heated. When only a part of the gene samples in the sample box 1201 needs to be heated, without taking out the samples that need to be heated separately, the heating mode of the first heating element is selected to heat the gene samples that need to be heated in the sample box 1201, and other gene samples that do not need to be heated are not affected.

[0139] In some embodiments: the first heating element includes a metal block 1205, a heating rod is installed in the metal block 1205, the upper surface of the metal block 1205 contacts the bottom of the sample position to be heated in the sample box 1201 to heat the genetic sample in the sample position, and the sample positions of the sample box 1201 are all arranged in a rectangular array in the sample box 1201.

[0140] When it is necessary to heat all the gene samples in the sample box 1201, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1201; the gene samples in the same row of sample positions are regarded as a group, and the conditions of all processes in the same group of gene samples should be exactly the same when conducting experimental tests to avoid exceptions affecting the final results. However, some conditions of different groups of gene samples need to be different when conducting experimental tests. Therefore, when performing heating treatment, it may be necessary to heat only one or more groups of gene samples in the sample box 1201. When the heating mode of the first heating element is selected to heat the gene samples in the sample box 1201 that need to be heated without taking out the samples that need to be heated separately, the sample box 1201 is placed in the first heating chamber 1203, so that the bottom of the sample position of the row of gene samples that need to be heated contacts the upper surface of the metal block 1205 to heat the gene samples in the row of sample positions, and at the same time, it will not affect other gene samples that do not need to be heated.

[0141] In some embodiments: a plurality of heating grooves 1202 are disposed on the upper surface of the metal block 1205, and the bottom of each sample placement position of the sample box 1201 bulges outward to form a boss 1206, and a heating groove 1202 contacts the boss 1206 at the bottom of a sample placement position to heat the gene sample in the sample placement position.

[0142] The boss 1206 at the bottom of the row of sample positions of the sample box 1201 contacts the heating groove 1202 on the metal block 1205, increasing the contact area between the boss 1206 at the bottom of the row of sample positions and the metal block 1205, thereby accelerating the heating efficiency of the metal block 1205 on the gene sample.

[0143] In some embodiments, the heating groove 1202 on the metal block 1205 and the boss 1206 at the bottom of the sample placement position are both hemispherical.

[0144] By making the heating groove 1202 on the metal block 1205 and the boss 1206 at the bottom of the sample placement position both hemispherical, the contact area between the boss 1206 at the bottom of the sample placement position and the metal block 1205 is further increased, further accelerating the heating efficiency of the metal block 1205 on the gene sample.

[0145] In some embodiments: the first heating element includes a placement table 1207 and a thermal radiation infrared light source, at least one through groove 1208 is set on the placement table 1207, the sample placement position that needs to be heated in the sample box is located at one end of the through groove 1208, and the thermal radiation infrared light source is placed at the other end of the through groove 1208, and the thermal radiation infrared light source passes through the through groove 1208 to heat the genetic sample in the sample placement position.

[0146] When it is necessary to heat all the gene samples in the sample box 1201, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1201; the gene samples in the same row of sample positions are regarded as a group, and the conditions of all processes in the same group of gene samples should be exactly the same when conducting experimental tests to avoid exceptions affecting the final results. However, different groups of gene samples need to have different conditions when conducting experimental tests. Therefore, when performing heating treatment, it may be necessary to heat only one or more groups of gene samples in the sample box 1201, and the samples that need to be heated do not need to be heated. When taking out individually, the heating mode of the first heating element is selected to heat the gene samples that need to be heated in the sample box 1201, and the sample box 1201 is placed in the first heating chamber 1203, so that the bottom of the sample placement position of the row of gene samples that need to be heated is placed directly above the through groove 1208 of the placement table 1207, and the thermal radiation infrared light source is placed directly below the through groove 1208 of the placement table 1207. The infrared light generated by the thermal radiation infrared light source is parallel to the through groove 1208. The thermal radiation infrared light source passes through the through groove 1208 to heat the gene samples in the sample placement position of this row, and at the same time will not affect other gene samples that do not need to be heated.

[0147] like Figure 21-24 As shown, the heat sealer 16 comprises:

[0148] The sealing plate 1603 is a hollow structure, a plastic film for sealing the sample plate is fixed in the middle of the sealing plate 1603, and at least two positioning openings 1612 are provided on the sealing plate 1603;

[0149] A heating plate 1604 for heating the plastic film; the hollow size of the film sealing plate 1603 is larger than the heat sealing surface size of the heating plate 1604, and the sample plate is located directly below the heat sealing surface of the heating plate 1604;

[0150] A force applying member for controlling the heating plate 1604 to move away from or approach the film sealing plate 1603;

[0151] A reciprocating member 1019 is used to move the film sealing plate 1603 to between the heating plate 1604 and the sample plate; the moving direction of the film sealing plate 1603 is perpendicular to the moving direction of the heating plate 1604 .

[0152] The force-applying member includes a fixed plate 1606 and a cylinder 1608; the fixed plate 1606 and the heating plate 1604 are both provided with four through holes, the heating plate 1604 and the fixed plate 1606 are connected by bolts, and the screw of each bolt is provided with an elastic member 1016, and the two ends of the elastic member 1016 are respectively pressed and contacted with the opposite sides of the fixed plate 1606 and the heating plate 1604; the piston rod of the cylinder 1608 is fixedly connected with the fixed plate 1606; the reciprocating moving member 1019 includes a tray B1601, two linear guide rails 1607, two sliders 1602, and an installation Base 1610, synchronous belt transmission mechanism 1611; the power output end of the synchronous belt transmission mechanism 1611 is fixedly connected to the pallet B1601, and two sliders 1602 are fixedly installed on the mounting base 1610; a linear guide 1607 is slidably installed on a slider 1602; the two linear guides 1607 are respectively fixed on both sides of the pallet B1601; the film sealing plate 1603 is placed on the pallet B1601, and the pallet B1601 is provided with a photoelectric sensor A1609 for detecting whether the film sealing plate 1603 is placed on the pallet B1601.

[0153] The elastic part is spring B1605.

[0154] The mounting base 1610 is mounted on the operating platform 9. An opening A is provided on the operating platform 9 at the bottom of the mounting base 1610. A lifting device A1613 is provided on the operating platform 9 below the opening A. The transport rack A of the PCR plate transport module and the transport rack B of the sealing plate 1603 transport module can both be arranged between the mounting base 1610 and the operating platform 9. The moving path of the transport rack B is directly above the moving path of the transport rack A. The lifting device A1613 is used to lift the PCR plate on the transport rack A to the transport rack B for placement.

[0155] The plastic film is fixed on the sealing plate 1603 on all sides, and the plastic film is heated by the heating plate 1604 and a downward force is applied to the plastic film so that the plastic film is heat-sealed on the sample plate, thereby forming a relatively fixed connection relationship between the sample plate, the plastic film and the sealing plate 1603. Since the hollow size of the sealing plate 1603 is larger than the heat-sealing surface size of the heating plate 1604, the heat-sealing surface of the heating plate 1604 has no contact with the outer frame of the sealing plate 1603 during the heat-sealing process, and the outer frame of the sealing plate 1603 will not be thermally deformed. Since the positioning opening 1612 is arranged on the outer frame of the sealing plate 1603, even if the sample plate is deformed due to heat during the heat-sealing process, it will not affect the positioning accuracy of the subsequent processes.

[0156] like Figure 25-26As shown, the gene detection equipment includes a blocking device 18, and a partition 17 for preventing aerosol contamination is arranged on the operating platform 9 between the metal bath module 10 and the heat sealer. A through hole is opened on the partition 17, and the blocking device is used to control the opening and closing of the through hole.

[0157] The blocking device 18 includes a blocking motor 181, a connecting block 182, a rotating wheel 183, a connecting rod 184, a baffle 186, a slide module 187, and a slide rail module 188. The first end of the connecting rod 184 is provided with a penetrating slide groove 185. The blocking motor 181 and the slide rail module 188 are fixedly mounted on the operating platform 9. The rotating shaft of the blocking motor 181 is connected to one end of the connecting block 182, and the other end of the connecting block 182 is connected to the rotating wheel 183. The rotating wheel 183 can slide Installed in the sliding groove 185, the second end of the connecting long rod 184 is connected to the baffle 186, the slide slot module 187 is fixedly installed on the second end side wall of the connecting long rod 184, the slide rail module 188 slides with the slide slot module 187, the direction of the sliding groove 185 is consistent with the length direction of the connecting long rod 184, the connecting long rod 184 and the slide slot module 187 and the slide rail module 188 are all vertical, when the blocking motor 181 rotates, the baffle 186 is lifted or lowered, and the baffle 186 is used to open or block the through hole.

[0158] The blocking motor 181 in this embodiment is controlled by a microprocessor. When the PCR plate needs to pass through the partition 17, the blocking motor 181 is controlled to work. The rotating shaft of the blocking motor 181 drives the connecting block 182 to rotate. The rotation of the connecting block 182 drives the rotating wheel 183 to roll along the sliding groove 185. The sliding groove module 187 slides upward along the sliding rail module 188, driving the baffle 186 to move upward to expose the through hole on the partition 17; otherwise, the blocking motor 181 reverses, and the baffle 186 moves down to block the through hole on the partition 17.

[0159] like Figure 27-31 As shown, the metal bath module 10 includes a heat cover assembly 101, and the heat cover assembly 101 includes a metal heat cover 1013. The metal heat cover 1013 is provided with a plurality of through holes 1012. When the metal bath is carried out, the central axis of a through hole 1012 coincides with the central axis of a sample placement position of the PCR plate.

[0160] The thermal cover assembly 101 also includes:

[0161] Positioning piece; the positioning piece includes at least two positioning pins 1018, the two positioning pins 1018 and the PCR plate are always in a relatively static state, at least two positioning holes 1015 are provided on the metal thermal cover 1013, at least one positioning hole 1015 and the other positioning holes 1015 are located on different sides of the metal thermal cover 1013, and during positioning, one positioning pin 1018 is inserted into one positioning hole 1015.

[0162] Mounting bracket A1014 and elastic member 1016; two ends of the elastic member 1016 are respectively connected to the mounting bracket A1014 and the metal thermal cover 1013. When the metal thermal cover 1013 contacts the PCR plate, the direction in which the elastic member 1016 recovers its deformation is the same as the direction of gravity.

[0163] At least two connecting rods 8621; the first end of each connecting rod 8621 is fixedly connected to the metal heat cover 1013, the mounting bracket A1014 is provided with a plurality of mounting holes, the elastic member 1016 includes a plurality of springs 8623, a connecting rod 8621 passes through a mounting hole, a spring 8623 is sleeved on the outer wall of a connecting rod 8621, and the cross-sectional size of the mounting hole is larger than the cross-sectional size of the connecting rod 8621.

[0164] At least two limit blocks 1011 ; the cross-sectional dimension of the limit block 1011 is larger than the cross-sectional dimension of the mounting hole, and the second end of the connecting rod 8621 is fixedly connected to the limit block 1011 .

[0165] The thermal cover assembly 101 includes a moving part 1019 installed at the lower part of the operating platform 9. An opening B is provided on the operating platform 9 at an upper position corresponding to the moving part 1019. The moving path of the transport rack B passes through the bottom of the metal thermal cover 1013. The moving part 1019 moves upward to lift the PCR plate on the transport rack B of the sealing plate transport module until it is connected to the metal thermal cover 1013.

[0166] In this embodiment, a plurality of through holes 1012 are formed on the metal thermal cover 1013, and the central axis of the through hole 1012 coincides with the central axis of the sample placement position of the reaction plate, so that when the reaction plate is performing a PCR amplification reaction in the metal bath, the light used for fluorescence detection can still reach the gene sample in the sample placement position, thereby achieving real-time detection of the gene sample during the PCR amplification reaction, so as to obtain more comprehensive detection results.

[0167] The optical detector 11 includes a light filtering device 111 . The optical detector 11 is installed on the upper part of the thermal cover assembly 101 and acts on the PCR plate after passing through the through hole 1012 .

[0168] like Figure 32-37 As shown, the optical filtering device 111 comprises:

[0169] A dichroic mirror 1115 for reflecting the light source; the incident angle of the light source on the dichroic mirror 1115 is an acute angle;

[0170] Light receiving box 1114; the light receiving box 1114 is used to absorb the refracted light 1117 on the side of the incident light 1117 on the dichroic mirror 1115 whose normal is far away from the light receiving box 1114. A light through hole 1116 is provided at the upper end of the light receiving box 1114. The dichroic mirror 1115 is installed at the upper end of the light receiving box 1114. The light 1117 absorbing end of the light receiving box 1114 and the excitation light source are located on both sides of the dichroic mirror 1115.

[0171] The side walls and bottom surface of the light receiving box 1114 are both spray-coated black.

[0172] When the light source passes through the dichroic mirror 1115, it is refracted and reflected by the dichroic mirror 1115, and the refracted light 1117 enters the light receiving box 1114. Since the side walls and the bottom surface inside the light receiving box 1114 are spray-coated black, the refracted light 1117 can be absorbed to avoid the light 1117 entering the light receiving box 1114 being refracted again after being reflected by the light receiving box 1114 and mixing with the light 1117 reflected for the first time by the dichroic mirror 1115, thereby reducing the accuracy of genetic testing. Therefore, the absorption of the light 1117 by the light receiving box 1114 reduces the presence of stray light and improves the accuracy of genetic testing.

[0173] The angle between the bottom surface and the side wall inside the light receiving box 1114 is not equal to 90°.

[0174] The bottom surface inside the light receiving box 1114 is tilted so that the light 1117 reaching the bottom surface of the light receiving box 1114 after refraction is reflected again onto the side wall of the light receiving box 1114, thereby increasing the light absorption effect of the light receiving box 1114 and further improving the accuracy of detection.

[0175] The inside of the light receiving box 1114 is filled with black porous sponge. When the light source passes through the two-way mirror 1115, it is refracted and reflected by the two-way mirror 1115, and the refracted light 1117 enters the light receiving box 1114. Since the side walls and bottom surface of the light receiving box 1114 are both sprayed with black plastic, the refracted light 1117 can be absorbed to avoid the light 1117 entering the light receiving box 1114 being refracted again after being reflected by the light receiving box 1114 and mixing with the light 1117 reflected by the two-way mirror 1115 for the first time, thereby reducing the accuracy of genetic testing. Therefore, the absorption of the light 1117 by the light receiving box 1114 reduces the existence of stray light and improves the accuracy of genetic testing.

[0176] The optical filtering device 111 further includes:

[0177] A first filter 1111 for filtering other wavelengths of light; the first filter 1111 is fixedly mounted on a mounting bracket B1112, and the first filter 1111 is located above a dichroic mirror 1115;

[0178] A second filter 1113; a mounting hole is provided at the upper end of the light receiving box 1114, and the filter is mounted in the mounting hole of the light receiving box 1114;

[0179] Total reflection mirror 1118; the reflection surface of the total reflection mirror 1118 faces the dichroic mirror 1115, and the light 1117 after total reflection is parallel to the central axis of the PCR plate 95 being detected.

[0180] The initial emission light source filters the light 1117 of other wavelengths through the first filter 1111, reaches the dichroic mirror 1115, is reflected and refracted by the dichroic mirror 1115, and the reflected light 1117 passes through the light hole 1116 on the light receiving box 1114 to reach the total reflection mirror 1118, and after being reflected by the total reflection mirror 1118, reaches the PCR plate 95 and irradiates the gene sample in the PCR plate 95. The light 1117 reaching the PCR plate 95 is parallel to the central axis of the PCR plate 95, and then the gene sample in the PCR plate 95 is irradiated. Because the reflected light of the sample returns along the original path, it is reflected by the total reflection mirror 1118 and reaches the dichroic mirror 1115, and the dichroic mirror 1115 refracts and reflects again. Since the incident direction of the light 1117 this time is located at the end of the normal of the dichroic mirror 1115 close to the light receiving box 1114, the reflected light 1117 will not be absorbed by the light receiving box 1114, and even if it is absorbed, only a very small part of it. Therefore, the refracted light 1117 here can be collected through the second filter 1113 for subsequent analysis.

[0181] The reflecting surface of the total reflection mirror 1118 is parallel to the dichroic mirror 1115, and the acute angle between the reflecting surface of the total reflection mirror 1118 and the dichroic mirror 1115 and the gravity direction is 45°, which ensures that the reflected light 1117 of the total reflection mirror 1118 is parallel to the incident light 1117 of the dichroic mirror 1115. Therefore, it is only necessary to direct the light 1117 of the initial emitting light source toward the central axis of the PCR plate 95 to ensure that the light 1117 reaching the PCR plate 95 is parallel to the central axis of the PCR plate 95.

[0182] The central axis of the second filter 1113 is perpendicular to the central axis of the light receiving box 1114 .

[0183] like Figure 1 , 2As shown in Figure 3, in some embodiments, the genetic testing device also includes a shell 1, a storage window 2, an observation window 3, a liquid circuit unit 4, a power supply unit 5, a recovery port A6, and a recovery port B7. The operating platform 9 is installed at the lower part of the shell 1, and the liquid circuit unit 4 is installed on the back of the shell 1 and is connected to the liquid pipeline on the pipetting component 8; the power supply unit 5 is installed at the upper part of the shell 1 and supplies power to all devices of the genetic testing device that need power; the recovery port A6 and the recovery port B7 are both opened on the shell 1, and the recovery port A6 is connected to the gun tip recovery component; the storage window 2 is opened at one end of the shell 1, and the storage window 2 is used to put the reagent into the purification and heating device. The observation window 3 is opened on one side of the shell 1, and the actuation of the pipetting component 8 and the actuation of the heat sealer can be observed through the observation window 3.

[0184] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A gene detection device, characterized in that: include: A pipetting assembly, an operating platform, and reagents, samples, a gun tip processing module, a purification and heating device, a metal bath module, an optical detector, a PCR plate transport module, a film sealing plate transport module, and a heat sealer arranged on the operating platform; Wherein, the pipetting assembly is located above the level of the operating platform; The metal bath module includes a heat cover assembly, the heat cover assembly includes a metal heat cover, the metal heat cover is provided with a plurality of through holes, when the metal bath is carried out, the central axis of a through hole coincides with the central axis of a sample placement position of the PCR plate; The optical detector includes a light filtering device, which is mounted on the upper part of the thermal cover assembly and acts on the PCR plate after passing through the through hole. The light filtering device includes a dichroic mirror and a light receiving box for reflecting the light source; the incident angle of the light source on the dichroic mirror is an acute angle; the light receiving box is used to absorb the refracted light of the incident light on the dichroic mirror on the side where the normal is far away from the light receiving box, and a light through hole is provided at the upper end of the light receiving box. The dichroic mirror is mounted on the upper end of the light receiving box, and the light absorbing end of the light receiving box and the excitation light source are located on both sides of the dichroic mirror; The purification and heating device comprises: At least one first heating element; the heating end of the first heating element acts on the genetic samples with the same heating requirements in the sample box; a first heating chamber; the first heating element is installed in the first heating chamber; A second heating element; the heating end of the second heating element acts on all the gene samples in the sample box, and the number of gene samples acted on by the heating end of the first heating element is less than the number of gene samples acted on by the heating end of the second heating element; The second heating chamber; the second heating element is installed in the second heating chamber, and when heating gene samples with the same heating requirements, the sample box is placed in the first heating chamber, and when heating all gene samples, the sample box is placed in the second heating chamber; The second heating element is a heating base, and a heating plate is installed inside the heating base. When all the gene samples are heated, the upper surface of the heating base contacts the bottom of the sample box to heat all the gene samples in the sample box; The upper surface of the heating base is provided with a plurality of heating grooves, the bottom of each sample placement position of the sample box is convex to form a boss, and one of the heating grooves contacts with a boss at the bottom of the sample placement position to heat the gene sample in the sample placement position; The first heating element comprises a metal block, a heating rod is installed in the metal block, and when heating a gene sample with the same heating requirement, the upper surface of the metal block contacts the bottom of the sample position to be heated in the sample box, so as to heat the gene sample in the sample position; The upper surface of the metal block is provided with a plurality of heating grooves, the bottom of each sample placement position of the sample box is convex to form a boss, and one of the heating grooves contacts with a boss at the bottom of the sample placement position to heat the gene sample in the sample placement position; The optical filtering device further comprises: A first filter for filtering other wavelengths of light; the first filter is located above the dichroic mirror; A second filter; a mounting hole is provided at the upper end of the light receiving box, and the second filter is mounted in the mounting hole of the light receiving box; and, A total reflection mirror; the reflection surface of the total reflection mirror faces the dichroic mirror, and the light after total reflection by the total reflection mirror is parallel to the central axis of the sample box being detected.

2. The gene detection device according to claim 1, characterized in that: The genetic testing device also includes: a tray module, which includes a tray and a linear motor assembly A, and the linear motor assembly A is installed on the operating platform. The tray is connected to the moving part of the linear motor assembly A. Within the movement range of the tray, the tray is synchronized with the lateral movement of the gun tip on the pipetting assembly, and the tray is always placed under the gun tip of the pipetting assembly.

3. The gene detection device according to claim 1, characterized in that: The pipetting assembly comprises: An X-direction moving assembly for moving the pipetting action part along the X-direction; the X-direction moving assembly comprises an X-direction moving guide rail A, an X-direction moving guide rail B, and an X-direction electric cylinder; the X-direction moving guide rail A and the X-direction moving guide rail B are parallel; the Y-direction moving assembly is fixedly mounted on the sliding part of the X-direction electric cylinder; A Y-direction moving assembly for moving the liquid transfer action part in the Y-direction; the Y-direction moving assembly comprises a bearing plate, a support A, a support B, a screw A, a Y-direction moving part, an X-direction motor, a pulley A, a pulley B, and a screw sleeve A; the support A and the support B are respectively mounted at two ends of the bearing plate, and two ends of the screw A can be rotatably mounted on the support A and the support B; the screw sleeve A is mounted on the Y-direction moving part, the screw sleeve A is threadedly mounted with the screw A, the pulley B is fixedly connected to one end of the screw A, the X-direction motor is mounted on the bearing plate, the rotating shaft of the bearing plate is fixedly connected to the pulley A, and the pulley A is connected to the pulley B through a belt transmission; A Z-direction moving assembly for moving the pipetting action part along the Z direction; the Z-direction moving assembly includes a Z-direction moving assembly B for moving the steel needle group A along the Z direction, a Z-direction moving assembly C for moving the steel needle group B along the Z direction, a Z-direction moving assembly D for moving the gun head along the Z direction, and a Z-direction moving assembly A for moving the Z-direction moving assembly B, the Z-direction moving assembly C and the Z-direction moving assembly D along the Z direction; Among them, the Y-axis moving component is installed on the moving part of the X-axis moving component, the Z-axis moving component A is installed on the moving part of the Y-axis moving component, and the Z-axis moving component B, the Z-axis moving component C and the Z-axis moving component D are all installed on the moving part of the Z-axis moving component A.

4. The gene detection device according to claim 3, characterized in that: The Z-direction moving assembly A comprises a frame A, a horizontal plate A, a Z-direction motor A, a screw rod B, a screw rod sleeve B, and a frame B; the frame A is fixedly mounted on the moving part of the Y-direction moving assembly, the horizontal plate A is fixedly mounted in the frame A, the Z-direction motor A is invertedly mounted on the horizontal plate A, the rotating shaft of the Z-direction motor A passes through the horizontal plate A and is fixedly connected to one end of the screw rod B, the screw rod sleeve B is threadedly mounted with the screw rod B, and the screw rod sleeve B is fixedly mounted on the frame B; the frame B is movable up and down and is placed in the frame A and is located below the horizontal plate A; The Z-axis moving assembly B includes a Z-axis motor B, a horizontal plate B, a guide rod, a screw rod C, a frame C, a screw rod sleeve C, and a steel needle group A. The horizontal plate B is fixedly installed in the frame B, the Z-axis motor B is invertedly installed on the horizontal plate B, the rotating shaft of the Z-axis motor B passes through the horizontal plate B and is fixedly connected to one end of the screw rod C, the screw rod sleeve C is threadedly installed with the screw rod C, and the screw rod sleeve C is fixedly installed on the frame C; the frame C is movable up and down and is placed in the frame B and is located below the horizontal plate B, the steel needle group A is installed on the frame C, and the steel needle group A is connected to the external fluid circuit; The Z-direction moving assembly C includes a connecting piece, a housing, a Z-direction motor C, a steel needle group B, a mounting plate, a high-frequency valve, a pipe winding device, a screw rod D, and a screw rod sleeve D. The connecting piece is connected to the frame B, the Z-direction motor C is mounted on the connecting piece, the steel needle group B, the high-frequency valve, the pipe winding device, and the screw rod sleeve D are all mounted on the mounting plate, the Z-direction motor C is mounted in an inverted vertical position, the rotating shaft of the Z-direction motor C is fixedly connected to one end of the screw rod D, the screw rod sleeve D is threadedly mounted to the screw rod D, the external liquid circuit is connected to one end of the high-frequency valve, and the other end of the high-frequency valve is connected to the steel needle group B through the pipe winding device; The Z-direction moving assembly D comprises a gun head, a mounting box, a connecting rod, a clamping block, a spring, a piston chamber assembly, a piston assembly, a connecting plate, a screw sleeve E, a screw E, a frame D, a Z-direction motor D, a connector, and a tapered nozzle; the frame D is fixedly connected to the frame B, the Z-direction motor D is invertedly installed on the upper end of the frame D, the rotating shaft of the Z-direction motor D passes through the upper end of the frame D and is fixedly connected to one end of the screw E, the screw sleeve E is threadedly installed with the screw E, the screw sleeve E is fixedly installed on the upper end of the outer shell of the piston assembly, and the piston assembly is installed in the frame D so as to be movable up and down; the piston chamber assembly is fixedly installed in the frame D, the piston chamber assembly is placed below the piston assembly, and the piston chamber assembly is installed in cooperation with the piston assembly; the gun head is placed below the piston chamber assembly and is connected and cooperated with the piston chamber assembly; The Z-direction moving component D also includes an installation box, a connecting rod, a block, a spring, a connecting plate, and a connector; the connecting plate is arranged at the lower end of the piston assembly, and a stepped through hole is vertically opened on the connecting plate, the upper diameter of the stepped through hole is larger than the lower diameter, the upper end of the connecting rod is connected to the block, and the lower end of the connecting rod is fixedly connected to the installation box, the diameter of the block is slightly smaller than the stepped through hole, the spring is sleeved on the connecting rod, and the spring is installed between the step of the stepped through hole and the block; a plurality of through holes A are arranged on the installation box, and a plurality of connectors can be slidably installed in the through holes A, and a tapered pipe mouth is arranged at the lower end of the connector, and the tapered pipe mouth is inserted into the upper end of the gun head for installation; when the piston assembly is pressed down to the bottom, the piston assembly squeezes the block downward, the spring is compressed, the block slides into the upper hole of the stepped through hole, the connecting rod pushes the installation box to move downward, and the installation box pushes the gun head out of the tapered pipe mouth.

5. The gene detection device according to claim 1, characterized in that: The reagent, sample, and gun tip processing module includes a gun tip storage module for gun tip storage, a sample cooling module, a reagent cooling module A, a reagent cooling module B, a needle cleaning module A, a needle cleaning module B, a positive reference cooling module, and a gun tip recovery component. The gun tip storage module, the sample cooling module, the reagent cooling module A, the needle cleaning module A, the needle cleaning module B, the reagent cooling module B, the positive reference cooling module, and the gun tip recovery component are all installed on the operating platform; the needle cleaning module A is used for cleaning the steel needle group A, and the needle cleaning module B is used for cleaning the steel needle group B.

6. The gene detection device according to claim 1, characterized in that: The heat sealing machine comprises: A heating plate for heating the plastic film, wherein the PCR plate is located directly below the thermal cover of the heating plate; A force applying member for controlling the heating plate to move away from or close to the PCR plate; A reciprocating member used to move the sealing plate between the heating plate and the PCR plate.

7. The gene detection device according to claim 6, characterized in that: The force-applying member includes a fixed plate and a cylinder; four through holes are provided on the fixed plate and the heating plate, and the heating plate and the fixed plate are connected by bolts, and an elastic member is mounted on the screw rod of each bolt, and the two ends of the elastic member are respectively squeezed and contacted with the opposite sides of the fixed plate and the heating plate; the piston rod of the cylinder is fixedly connected to the fixed plate; the reciprocating moving member includes a tray, two linear guides, two sliders, a mounting base, and a synchronous belt transmission mechanism; the power output end of the synchronous belt transmission mechanism is fixedly connected to the tray, and the two sliders are fixedly installed on the mounting base; a linear guide can be slidably installed on a slider; the two linear guides are respectively fixed on both sides of the tray.

8. The gene detection device according to claim 7, characterized in that: The mounting base is mounted on the operating platform, an opening A is provided on the operating platform corresponding to the bottom of the mounting base, a lifting device A is provided correspondingly below the opening A of the operating platform, a transport rack A of the PCR plate transport module and a transport rack B of the sealing plate transport module can both be arranged through between the mounting base and the operating platform, a moving path of the transport rack B is above the level of the moving path of the transport rack A, and the lifting device A is used to lift the PCR plate on the transport rack A to the transport rack B for placement.

9. The gene detection device according to claim 1, characterized in that: It also includes a partition and a blocking device. The partition is arranged between the metal bath module and the heat sealer to isolate the metal bath module from the heat sealer. A through hole is opened on the partition, and the blocking device is used to control the opening and closing of the through hole.

10. The gene detection device according to claim 9, characterized in that: The blocking device includes a blocking motor, a connecting member, a rotating wheel, a connecting rod, a baffle, a slide module, and a slide rail module. A penetrating sliding groove is opened on the first end of the connecting rod. The blocking motor and the slide rail module are fixedly installed on the operating platform. The rotating shaft of the blocking motor is connected to one end of the connecting member, and the other end of the connecting member is connected to the rotating wheel. The rotating wheel can be slidably installed in the sliding groove. The second end of the connecting rod is connected to the baffle, and the slide module is fixedly installed on the second end side wall of the connecting rod. The slide rail module slides with the slide module, and the direction of the sliding groove is consistent with the length direction of the connecting rod. The connecting rod is perpendicular to the slide module and the slide rail module. When the blocking motor rotates, the baffle is lifted or lowered to open or block the through hole.

11. The gene detection device according to claim 1, characterized in that: The thermal cover assembly further comprises: Positioning member; the positioning member includes at least two positioning pins, the two positioning pins and the PCR plate are always in a relatively static state, the metal thermal cover is provided with at least two positioning holes, at least one positioning hole and the other positioning holes are located on different sides of the metal thermal cover, and when positioning, one positioning pin is inserted into one positioning hole; The mounting bracket and the elastic member are installed; the two ends of the elastic member are connected to the mounting bracket and the metal thermal cover respectively, and when the metal thermal cover contacts the PCR plate, the direction in which the elastic member recovers its deformation is the same as the direction of gravity; At least two connecting rods; the first end of each connecting rod is fixedly connected to the metal heat cover, the mounting bracket is provided with a plurality of mounting holes, the elastic member comprises a plurality of springs, a connecting rod passes through a mounting hole, a spring is sleeved on the outer wall of a connecting rod, and the cross-sectional size of the mounting hole is larger than the cross-sectional size of the connecting rod; At least two limit blocks; the cross-sectional size of the limit block is larger than the cross-sectional size of the mounting hole, and the second end of the connecting rod is fixedly connected to the limit block.

12. The gene detection device according to claim 11, characterized in that: The thermal cover assembly includes a moving part installed at the lower part of the operating platform. An opening B is opened on the operating platform corresponding to the upper position of the moving part. The moving path of the transport rack B passes through the bottom of the metal thermal cover. The moving part moves upward to lift the PCR plate on the transport rack B of the sealing plate transport module until it is connected to the metal thermal cover.

13. The gene detection device according to claim 1, characterized in that: The angle between the bottom surface and the side wall inside the light receiving box is not equal to 90°.

14. The gene detection device according to claim 1, characterized in that: The genetic testing equipment also includes a PCR plate recovery component, which includes a paddle. An opening C is provided on the table top of the operating platform corresponding to the bottom of the paddle. The PCR plate placed on the transport rack B of the transport module contacts the paddle. The paddle drives the PCR plate to tilt and fall into the opening C and is recovered through the recovery port B.

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