An odor diffusion device for insect odor training

Abstract

The present invention discloses an odor diffusion device for insect odor training. The odor diffusion device includes an odor container, an insect container, an intermediate connector, a flowmeter, and an air extraction component. The odor container is used to contain experimental reagents, and the odor container is provided with an odor channel. The insect container has a containing cavity for containing insects, and one end of the containing cavity is communicated with the odor channel. The intermediate connector has an air flow channel, one end of the air flow channel is connected to the other end of the containing cavity, the gas inlet of the flowmeter is connected to the other end of the air flow channel, and the air extraction component is connected to the gas outlet of the flowmeter. This odor diffusion device can be used to study the influence of gases with different concentrations on the selection behavior of insects, can also study the influence of the length of contact time with the gas on the selection behavior of insects, and can also better avoid experimental errors caused by odor molecule cross-taste.

Description

Technical Field

[0001] The present invention relates to the technical field of insect experimental devices, and particularly to an odor diffusion device for insect odor training. Background Art

[0002] Insects such as Drosophila have a good sense of smell and are very suitable for studying the neural mechanisms of olfactory learning and memory. For the learning and memory training of insects, specific odors are usually required to be given. Most previous studies used a T-shaped device for Drosophila olfactory training. Different odors were introduced into the left and right sides of the T-shaped pipeline. Drosophila crawled in the T-shaped device and made odor choices according to their preferences. The defect of this method is that different odors cannot be completely isolated, and the distance between the odor source and Drosophila is relatively far, and the diffusion rate of odor molecules cannot be guaranteed accurately. If the diffusion rate of odor molecules is slow, the effect of allowing Drosophila to make choices cannot be achieved. If the diffusion of odor molecules affects each other and cross-taste occurs, it will cause errors in the experimental results. Summary of the Invention

[0003] The purpose of the present invention is to provide an odor diffusion device for insect odor training. The odor diffusion device can be used to study the influence of gases with different concentrations on the selection behavior of insects, can also study the influence of the length of contact time with gases on the selection behavior of insects, and can also better avoid experimental errors caused by cross-taste of odor molecules.

[0004] To achieve the above technical effects, the technical solution of the present invention is as follows:

[0005] The present invention discloses an odor diffusion device for insect odor training, including: an odor containing member for containing experimental reagents, and the odor containing member is provided with an odor channel; an insect containing member having a containing cavity for containing insects, and one end of the containing cavity is communicated with the odor channel; an intermediate connecting member having an air flow channel, and one end of the air flow channel is connected to the other end of the containing cavity; a flow meter, the gas inlet of the flow meter is connected to the other end of the air flow channel; an air extraction member, the air extraction member is connected to the gas outlet of the flow meter; wherein: the odor containing member is detachably connected to the insect containing member, and the insect containing member is detachably connected to the intermediate connecting member.

[0006] In some embodiments, the air flow channels of the intermediate connecting member are multiple.

[0007] In some embodiments, the odor containing member includes: a main body provided with a containing groove and a first air hole, the containing groove is used for containing experimental reagents, and the first air hole can be communicated with the insect containing member; a cover body buckled on the main body to cover the containing groove, and the cover body is provided with a second air hole.

[0008] In some alternative embodiments, an installation groove is provided on the body, the installation groove communicates with the first air hole, and one end of the insect receiving member is inserted into the installation groove.

[0009] In some alternative embodiments, a receiving protrusion and an annular groove surrounding the receiving protrusion are provided on the body, a receiving groove is provided on the receiving protrusion, one end of the cover body is fitted in the annular groove, the first air hole is provided on the bottom wall of the annular groove, and a plurality of the first air holes are provided surrounding the receiving protrusion.

[0010] In some alternative embodiments, there are a plurality of the second air holes.

[0011] In some alternative embodiments, there are a plurality of the receiving grooves, and the cross-sections of the plurality of receiving grooves are different from each other.

[0012] In some embodiments, the insect receiving member includes a first section and a second section, the first section is connected to the intermediate connecting member, the second section is connected to the odor receiving member, and the diameter of the first section is smaller than that of the second section.

[0013] In some embodiments, a sealing ring is provided between the first section and the intermediate connecting member.

[0014] In some embodiments, a blocking net is provided at the open end of the second section away from the first section.

[0015] The odor diffusion device for insect odor training according to the embodiment of the present invention, due to the provision of the air extraction member and the flow member, realizes that the insects in the insect receiving member can receive gases with different concentrations and different speeds, so that the odor diffusion device of this embodiment can be applied to the research on the influence of gases with different concentrations on the selection behavior of insects and the influence of the length of contact time with the gas on the selection behavior of insects, improves the applicable range of the odor diffusion device, and the odor receiving member and the insect receiving member are detachably connected, which can preferably avoid the phenomenon of actual odor cross-talk in different experiments during the experiment on the influence of different odors on insects, and improves the experimental accuracy.

[0016] Additional aspects and advantages of the present invention will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a structural diagram of the odor diffusion device for insect odor training according to the embodiment of the present invention.

[0018] Figure 2 is a schematic structural diagram of the odor receiving member according to the embodiment of the present invention.

[0019] Figure 3 It is an exploded schematic view of the odor accommodating member according to an embodiment of the present invention.

[0020] Figure 4 It is a structural schematic view of the intermediate connecting member according to an embodiment of the present invention.

[0021] Reference numerals:

[0022] 1. Odor accommodating member; 11. Body; 111. Accommodating groove; 112. First air hole; 113. Installation groove; 114. Accommodating protrusion; 115. Annular groove; 12. Cover body; 121. Second air hole; 2. Insect accommodating member; 21. First section; 22. Second section; 3. Intermediate connecting member; 31. Air flow channel; 4. Flowmeter; 5. Air extraction member. Detailed implementation manners

[0023] To make the technical problems solved by the present invention, the technical solutions adopted and the achieved technical effects clearer, the technical solutions of the present invention will be further described below with reference to the drawings and through specific implementation manners.

[0024] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0025] In addition, the features defined as "first" and "second" may explicitly or implicitly include one or more of such features, used to distinguish and describe the features, without order or importance. In the description of the present invention, unless otherwise specified, the meaning of "a plurality" is two or more.

[0026] In the description of the present invention, it should be noted that, unless otherwise clearly defined and limited, the terms "installation", "connection" and "coupling" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be a direct connection or an indirect connection through an intermediate medium, and it may be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0027] Next, refer toFigures 1-4 Describe the specific structure of the odor diffusion device for insect odor training according to the embodiments of the present invention.

[0028] As Figures 1-4 shown, the present invention discloses an odor diffusion device for insect odor training. The odor diffusion device includes an odor container 1, an insect container 2, an intermediate connector 3, a flow meter 4, and an air extraction member 5. The odor container 1 is used to contain experimental reagents, and the odor container 1 is provided with an odor channel. The insect container 2 has a containing cavity for containing insects. One end of the containing cavity is communicated with the odor channel. The intermediate connector 3 has an air flow channel 31. One end of the air flow channel 31 is connected to the other end of the containing cavity. The gas inlet of the flow meter 4 is connected to the other end of the air flow channel 31. The air extraction member 5 is connected to the gas outlet of the flow meter 4. The odor container 1 is detachably connected to the insect container 2, and the insect container 2 is detachably connected to the intermediate connector 3.

[0029] It can be understood that during actual use, the experimental reagent with a specific odor is placed on the odor container 1, and then the insects are placed in the insect container 2. Then, the air extraction member 5, the flow meter 4, the intermediate connector 3, the insect container 2, and the odor container 1 are assembled in sequence. Then, the air extraction member 5 is started, and the external gas can enter the containing cavity through the odor channel to contact the experimental insects. During the experiment, by adjusting the air extraction member 5 and combining with the flow meter 4, the speed and time of the gas diffusing into the insect container 2 are regulated, so that the odor diffusion device of this embodiment can be used to study the influence of gases with different concentrations on the selection behavior of insects, or study the influence of the length of contact time with the gas on the selection behavior of insects, and further accurately realize the experiment on the selection ability of insects for odors at different concentrations and different speeds. At the same time, since the experimental reagent is placed in the odor container 1 of this embodiment, when it is necessary to change the type of the experimental reagent, the odor container 1 can be directly removed from the insect container 2 and replaced, which avoids the possible odor cross-talk phenomenon of different experimental reagents and improves the experimental accuracy.

[0030] The odor diffusion device for insect odor training according to the embodiments of the present invention, due to the provision of the air extraction member 5 and the flow member, enables the insects in the insect container 2 to receive gases with different concentrations and different speeds, so that the odor diffusion device of this embodiment can be applied to the research on the influence of gases with different concentrations on the selection behavior of insects and the influence of the length of contact time with the gas on the selection behavior of insects, improves the applicable range of the odor diffusion device, and since the odor container 1 and the insect container 2 are detachably connected, it can better avoid the phenomenon of different experimental reagents cross-talking during the experiment on the influence of different odors on insects, and improves the experimental accuracy.

[0031] In some embodiments, the materials of the insect container 2 and the odor container 1 only need to be water- and oil-resistant and airtight materials, and can be specifically selected according to actual needs. No specific limitations are made on the materials of the insect container 2 and the odor container 1 herein.

[0032] In some embodiments, as Figure 4 shown, there are multiple air flow channels 31 in the intermediate connector 3. Thus, during actual experiments, multiple insect containers 2 and odor containers 1 can be connected to the intermediate connector 3 simultaneously. That is to say, the odor diffusion device of this embodiment can conduct multiple groups of insect experiments simultaneously. And since multiple experimental reagents are located in multiple odor containers 1, the phenomenon of cross-taste that may occur between different experimental reagents is avoided, improving the experimental accuracy. It can be understood that the number and distribution of the air flow channels 31 on the intermediate connector 3 can be selected according to actual needs, and no specific limitations are made on the air flow channels 31 on the intermediate connector 3 herein.

[0033] In some embodiments, as Figures 2-3 shown, the odor container 1 includes a main body 11 and a cover 12. The main body 11 is provided with a receiving groove 111 and a first air hole 112. The receiving groove 111 is used to receive the experimental reagent, and the first air hole 112 can communicate with the insect container 2. The cover 12 is buckled on the main body 11 to cover the receiving groove 111, and the cover 12 is provided with a second air hole 121. It can be understood that during actual experiments, after the experimental reagent is placed in the receiving groove 111 and the cover 12 is covered, when the air extraction member 5 is started, the external gas enters the inside of the cover 12 through the second air hole 121. The air flow can carry away the odor of the experimental reagent, enter the accommodation cavity through the first air hole 112 and contact the experimental insects. On the one hand, this ensures that the odor of the experimental reagent can stably contact the experimental insects. On the other hand, it slows down the diffusion of the odor of the experimental reagent towards the experimental space and also reduces the contamination of the experimental reagent by external pollutants.

[0034] In some alternative embodiments, as Figure 3 shown, the main body 11 is provided with a mounting groove 113, and the mounting groove 113 communicates with the first air hole 112. One end of the insect container 2 is inserted into the mounting groove 113. Thus, while ensuring the communication between the insect container 2 and the main body 11, it not only ensures the connection stability between the insect container 2 and the main body 11, avoiding the disconnection of the insect container 2 from the main body 11, but also ensures the connection tightness between the insect container 2 and the main body 11, reducing the phenomenon that external gas enters the insect container 2 from the connection gap between the insect container 2 and the main body 11, and reducing the influence of external pollutants on the experimental results.

[0035] In some alternative embodiments, as Figure 3As shown, the main body 11 is provided with a receiving protrusion 114 and an annular groove 115 disposed around the receiving protrusion 114. The receiving protrusion 114 is provided with a receiving groove 111. One end of the cover body 12 is fitted in the annular groove 115. The first air hole 112 is provided on the bottom wall of the annular groove 115, and the first air holes 112 are multiple and disposed around the receiving protrusion 114. It can be understood that the receiving groove 111 is provided on the receiving protrusion 114, which on the one hand ensures the stability of the experimental reagent, and on the other hand facilitates the experimenter to replace the experimental reagent. In addition, one end of the cover body 12 being fitted in the annular groove 115 preferably ensures the connection stability and connection tightness between the cover body 12 and the main body 11, and avoids experimental errors caused by external air flow entering the main body 11 from the connection gap between the cover body 12 and the main body 11.

[0036] In some alternative embodiments, as Figure 3 shown, there are multiple second air holes 121. Thereby, the external air flow rate is increased, facilitating the experiment.

[0037] In some alternative embodiments, as Figure 3 shown, there are multiple receiving grooves 111, and the cross-sections of the multiple receiving grooves 111 are different from each other. It can be understood that according to the foregoing, during the experiment, it may be necessary to replace the experimental reagent. In this embodiment, the cross-sections of the multiple receiving grooves 111 are different from each other. The experimenter can put experimental reagents of different volumes into the receiving grooves 111 according to actual needs, and can record the positions where the experimental reagents are placed according to the shapes of the cross-sections of the receiving grooves 111, avoiding putting two different experimental reagents into the same receiving groove 111 during the process of replacing the experimental reagent, thereby avoiding the possible odor mixing phenomenon of different experimental reagents and improving the experimental accuracy.

[0038] In some embodiments, as Figure 1 shown, the insect receiving member 2 includes a first section 21 and a second section 22. The first section 21 is connected to the intermediate connecting member 3, and the second section 22 is connected to the odor receiving member 1. The diameter of the first section 21 is smaller than that of the second section 22. Thereby, both the connection stability between the insect receiving member 2 and the intermediate connecting member 3 can be ensured, and the insect receiving member 2 can accommodate a sufficient number of experimental insects.

[0039] In some embodiments, a sealing ring is provided between the first section 21 and the intermediate connecting member 3. Thereby, both the stability between the first section 21 and the intermediate connecting member 3 can be ensured, avoiding the insect receiving member 2 falling off from the intermediate connecting member 3, and the connection tightness between the first section 21 and the intermediate connecting member 3 can be ensured, reducing the experimental error.

[0040] In some embodiments, a blocking net is provided at the open end of the second section 22 away from the first section 21. Thereby, it can preferably prevent the experimental insects from running out of the insect receiving member 2 into the odor receiving member 1.

[0041] In some embodiments, a blocking net is also provided between the intermediate connector 3 and the flowmeter 4 or at one end of the first section 21 away from the second section 22. Thus, it can better prevent experimental insects from running out of the insect accommodating member 2 into the intermediate connector 3 or the flowmeter 4.

[0042] Embodiment:

[0043] The following refers to Figures 1-4 Describe the specific structure of the odor diffusion device for insect odor training in a specific embodiment of the present invention.

[0044] The odor diffusion device includes an odor accommodating member 1, an insect accommodating member 2, an intermediate connector 3, a flowmeter 4, and an air extraction member 5. The odor accommodating member 1 is used to accommodate experimental reagents. The odor accommodating member 1 includes a main body 11 and a cover body 12. An installation groove 113 is provided on the side wall of the main body 11. A receiving protrusion 114 and an annular groove 115 surrounding the receiving protrusion 114 are provided on the top wall of the main body 11. Two receiving grooves 111 are provided on the receiving protrusion 114. The cross-sections of the two receiving grooves 111 are circular, and the cross-sectional diameters of the two receiving grooves 111 are not equal. Six first air holes 112 surrounding the receiving protrusion 114 are provided on the bottom wall of the annular groove 115. The first air holes 112 are round holes. One end of the cover body 12 is fitted in the annular groove 115. Seven second air holes 121 are provided on the cover body 12. Six of the second air holes 121 are arranged in one-to-one correspondence with the six first air holes 112, and the remaining one second air hole 121 is located at the circular origin surrounded by the six second air holes 121. The second air holes 121, the gap between the cover body 12 and the main body 11, and the first air holes 112 form a gas channel. The insect accommodating member 2 includes a first section 21 and a second section 22. The first section 21 is inserted into one air flow channel 31 of the intermediate connector 3, and the second section 22 is fitted in the installation groove 113. The diameter of the first section 21 is smaller than that of the second section 22. Four air flow channels 31 are provided on the intermediate connector 3. One end of each air flow is connected to the air extraction member 5 through the flowmeter 4.

[0045] The advantages of the odor diffusion device for insect odor training in this embodiment are as follows:

[0046] First: The air extraction member 5 can drive the air flow to disperse the odor of the experimental reagent throughout the insect accommodating member 2, so that the insects are completely exposed to a specific odor, forming a direct contact between the insects and the odor, and increasing the contact efficiency;

[0047] Second: The detachable connection between the odor accommodating member 1 and the insect accommodating member 2 can enable one odor accommodating member 1 to correspond to one odor, effectively isolate different odors, and avoid the mutual influence between different odors, thereby improving the experimental accuracy;

[0048] Thirdly, the speed and time of odor diffusion are controlled by the air extraction member 5 and the flowmeter 4, so that insects can be exposed to different odors and odors with different concentrations, enhancing the diversity of the experimental process;

[0049] Fourthly, the intermediate connecting member 3 can simultaneously install four insect accommodating members 2, realizing the function of carrying out four groups of experiments simultaneously and improving the work efficiency.

[0050] In the description of this specification, the descriptions referring to the terms "some embodiments", "other embodiments", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0051] The above content is only the preferred embodiment of the present invention. For those of ordinary skill in the art, according to the idea of the present invention, there will be changes in the specific implementation manner and application scope. The content of this specification should not be construed as a limitation to the present invention.

Claims

1. An odor diffusion device for insect odor training, characterized in that, Comprising: An odor accommodating member (1) for accommodating experimental reagents, and having an odor channel thereon; An insect accommodating member (2) having an accommodating cavity for accommodating insects, and one end of the accommodating cavity communicating with the odor channel; An intermediate connecting member (3) having an air flow channel (31), and one end of the air flow channel (31) being connected to the other end of the accommodating cavity; A flowmeter (4), the gas inlet of which is connected to the other end of the air flow channel (31); An air extraction member (5) connected to the gas outlet of the flowmeter (4); wherein: The odor accommodating member (1) is detachably connected to the insect accommodating member (2), and the insect accommodating member (2) is detachably connected to the intermediate connecting member (3); There are a plurality of air flow channels (31) in the intermediate connecting member (3), and each air flow channel is connected to an insect accommodating member (2) and an intermediate connecting member (3).

2. The odor diffusion device for insect odor training according to claim 1, wherein, The odor accommodating member (1) includes: A body (11) provided with an accommodating groove (111) and a first air hole (112) thereon, the accommodating groove (111) for accommodating experimental reagents, and the first air hole (112) capable of communicating with the insect accommodating member (2); A cover body (12) buckled on the body (11) to cover the accommodating groove (111), and a second air hole (121) is provided on the cover body (12).

3. The odor diffusion device for insect odor training according to claim 2, characterized in that, An installation groove (113) is provided on the body (11), the installation groove (113) communicating with the first air hole (112), and one end of the insect accommodating member (2) is inserted into the installation groove (113).

4. The odor diffusion device for insect odor training according to claim 3, characterized in that, An accommodating protrusion (114) and an annular groove (115) surrounding the accommodating protrusion (114) are provided on the body (11), the accommodating groove (111) is provided on the accommodating protrusion (114), one end of the cover body (12) is fitted in the annular groove (115), the first air hole (112) is provided on the bottom wall of the annular groove (115), and the first air hole (112) is a plurality of holes surrounding the accommodating protrusion (114).

5. The odor diffusion device for insect odor training according to claim 4, characterized in that, The second air holes (121) are a plurality of holes.

6. The odor diffusion device for insect odor training according to claim 2, characterized in that, There are a plurality of accommodating grooves (111), and the cross-sections of the plurality of accommodating grooves (111) are different from each other.

7. The odor diffusion device for insect odor training according to claim 1, characterized in that, The insect accommodating member (2) includes a first section (21) and a second section (22), the first section (21) being connected to the intermediate connecting member (3), the second section (22) being connected to the odor accommodating member (1), and the diameter of the first section (21) being smaller than the diameter of the second section (22).

8. The odor diffusion device for insect odor training according to claim 7, wherein A sealing ring is provided between the first section (21) and the intermediate connecting member (3).

9. The odor diffusion device for insect odor training according to claim 7, wherein, A blocking net is provided on the open end of the second section (22) away from the first section (21).

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