Microfluidic detection system for refrigerator and refrigerator

Abstract

The present invention relates to a microfluidic detection system for a refrigerator and a refrigerator. The microfluidic detection system includes: a microfluidic biochip, which is placed vertically and has a sample inlet, a communication port, and a detection pool formed inside thereof. The sample inlet is located at the bottom of the microfluidic biochip. The sample inlet, the detection pool, and the communication port are sequentially communicated through microchannels to form a main channel; a sample liquid driving device, which is adjacently arranged on the lateral side of the microfluidic biochip in the horizontal direction and is hermetically communicated with the communication port to promote the sample liquid in contact with the sample inlet to flow through the microchannels to the detection pool; and a detection mechanism, which is used to detect the detection pool after the sample liquid in the detection pool reacts with the detection reagent therein to obtain a preset detection parameter of the sample liquid. Thus, it not only ensures the compactness of the structural layout but also avoids the liquid from contacting the sample liquid driving device, thereby avoiding adverse effects on the sample liquid driving device.

Description

Technical Field

[0001] The present invention relates to refrigeration and freezing technologies, and particularly to a microfluidic detection system for a refrigerator and a refrigerator. Background Art

[0002] With the improvement of people's living standards, it is usually necessary to detect the pesticide residues, viruses, nutrient elements or other aspects of some food ingredients consumed in daily life to qualitatively or quantitatively obtain the conditions of the food ingredients. For example, due to the problem of pesticide abuse, the fruits, vegetables and agricultural and sideline products we buy daily may have the problem of excessive pesticide residue content. If the problem of excessive pesticide residue content in these foods cannot be detected in time, it will cause great harm to the human body after ingestion. Again, for the currently advocated breast-feeding, it is the best feeding for infants only when breast milk has normal nutritional value. However, in cases where the lactating mother is ill, takes medicine, has surgery or other situations, it may lead to a decrease in the content of nutrient elements in the milk secreted by her or even the generation of viruses, thus affecting the growth and health of the infant.

[0003] Among many detection methods, the method of detecting using a microfluidic biochip is relatively fast and has a small volume, which is suitable for home use. In order to make the sample injection of the microfluidic biochip more accurate and easy to control, a driving device can be used to drive the sample liquid into the microfluidic biochip. Existing detection systems generally exist independently. Therefore, each module such as its microfluidic biochip, driving device and detection device used to implement detection can be arranged at a relatively far distance from each other, without considering the mutual influence between each module. However, an independently existing detection system occupies space and is not convenient for storage. After storing the detection device, people may forget to use it, or may not take it out for use because they find it troublesome. For this reason, the applicant realized that the detection system can be integrated on the refrigerator. At this time, the structural layout between each module of the detection system is restricted, which may cause mutual influence between each module, thereby affecting the function or lifespan of the detection system. Summary of the Invention

[0004] An object of the first aspect of the present invention aims to overcome at least one defect of the prior art and provide a microfluidic detection system suitable for a refrigerator with a reasonable structural layout to eliminate adverse effects.

[0005] A further object of the first aspect of the present invention is to weaken the vibration of the microfluidic detection system and reduce its operating noise.

[0006] Another further object of the first aspect of the present invention is the accuracy of the sample liquid driving device for driving sample injection.

[0007] An object of the second aspect of the present invention is to provide a refrigerator having the above microfluidic detection system.

[0008] According to the first aspect of the present invention, the present invention provides a microfluidic detection system for a refrigerator, which includes:

[0009] A microfluidic biochip, placed vertically, and having a sample injection port, a communication port, and a detection pool formed inside thereof. The sample injection port is located at the bottom of the microfluidic biochip, and the sample injection port, the detection pool, and the communication port are sequentially connected through microchannels to form a main channel;

[0010] A sample liquid driving device, proximately arranged on the lateral side of the microfluidic biochip in the horizontal direction, and hermetically connected to the communication port to urge the sample liquid contacting the sample injection port to flow through the microchannels to the detection pool; and

[0011] A detection mechanism, used to detect the detection pool to obtain preset detection parameters of the sample liquid.

[0012] Optionally, the sample liquid driving device includes a driving motor, the driving motor is suspended, and the top of the driving motor is fixed on a bracket to support the driving motor through the bracket.

[0013] Optionally, the sample liquid driving device is a micro-injection pump, and further includes:

[0014] A vertically extending syringe, the syringe is fixed on the bracket, and the top of the syringe is hermetically connected to the communication port at the top of the microfluidic biochip through a connecting pipeline;

[0015] A lead screw, extending vertically and connected to the driving motor to rotate under the drive of the driving motor;

[0016] A slider, sleeved on the lead screw and threadedly connected to the lead screw to move up and down along the lead screw with the rotation of the lead screw; and

[0017] A piston, arranged inside the syringe and fixedly connected to the slider to move up and down under the drive of the slider, so that when it moves downwards, a negative pressure is generated in the main channel to urge the sample liquid contacting the sample injection port to flow into the microchannels and through the microchannels into the detection pool, and when it moves upwards, the sample liquid in the main channel is urged to flow towards the sample injection port.

[0018] Optionally, the microfluidic detection system further includes:

[0019] A position sensor, used to detect the position of the slider to control the operation of the driving motor through the position of the slider, so as to control the displacement amount of the piston moving upwards and / or downwards.

[0020] Optionally, a reaction pool for reacting the sample liquid and the reaction reagent is formed inside the microfluidic biochip. The reaction pool is located on the main channel and is connected between the injection port and the detection pool, so that the sample liquid first reacts with the reaction reagent in the reaction pool and then flows into the detection pool.

[0021] Optionally, the microfluidic channel connected between the communication port and the detection cell, and the microfluidic channel connected between the detection cell and the reaction cell both include a curved section extending in an S-shaped circuitous manner; and

[0022] The microchannel connected between the detection pool and the reaction pool is communicated with the upper end of the reaction pool, and the microchannel connected between the reaction pool and the injection port is communicated with the lower end of the reaction pool and extends in a vertical direction.

[0023] Optionally, the microfluidic channel connected between the communication port and the detection cell, and the microfluidic channel connected between the detection cell and the reaction cell both include a curved section extending in an S-shaped circuitous manner; and

[0024] The microfluidic channel connected between the detection pool and the reaction pool is communicated with the lower end of the reaction pool, and the microfluidic channel connected between the reaction pool and the injection port is communicated with the upper end of the reaction pool, so that the microfluidic channel connected between the detection pool and the reaction pool also includes a bending section between the bending section and the reaction pool, so that the microfluidic channel connected between the reaction pool and the injection port has a bending section.

[0025] Optionally, the communication port of the microfluidic biochip is sealed and connected to the sample liquid driving device via a sealing docking mechanism, and the sealing docking mechanism includes:

[0026] a sealing connector having a connection channel formed therein that passes through the sealing connector; and

[0027] an elastic pressure member for applying an elastic force to the sealing connector so that the sealing connector is simultaneously sealed and docked with the sample liquid driving device and one end of the communication port of the microfluidic biochip, thereby sealingly connecting the sample liquid driving device and the communication port through the connecting channel.

[0028] Optionally, the microfluidic detection system further includes:

[0029] A sample stage is provided below the microfluidic biochip for placing a sample cup, wherein the sample cup is used to hold a sample liquid;

[0030] The sample stage is arranged to move up and down controllably or operably, so as to convey the sample cup placed thereon upward through the sample stage to a position where the sample liquid in the sample cup is allowed to contact the sample inlet of the microfluidic biochip.

[0031] Optionally, the sample stage includes:

[0032] A support stage for supporting the sample cup; and

[0033] An oscillator arranged on the support stage for oscillating the sample cup after the sample cup is placed on the support stage, so that the buffer solution and the sample in the sample cup are fully mixed to generate the sample liquid.

[0034] According to the second aspect of the present invention, the present invention further provides a refrigerator, which includes the microfluidic detection system described in any of the above solutions.

[0035] The microfluidic detection system of the present invention includes a vertically placed microfluidic biochip and a sample liquid driving device. The sample inlet is located at the bottom of the microfluidic biochip, and the sample liquid driving device is adjacently arranged on the lateral side of the microfluidic biochip in the transverse direction. Thus, it not only ensures the compactness of the structural layout between the microfluidic biochip and the sample liquid driving device, but also enables the liquid to flow downward or drip along the microfluidic biochip when the liquid in the microfluidic biochip leaks or drains out, without contacting the sample liquid driving device, thereby avoiding adverse effects on the sample liquid driving device.

[0036] Furthermore, the sample liquid driving device has a driving motor, and the driving motor is supported by a bracket fixedly connected to its top, and the driving motor is suspended without contacting other structures, avoiding the vibration generated during the operation of the driving motor from being transmitted to the microfluidic biochip or other structures, thereby reducing the vibration of the entire microfluidic detection system and lowering its operating noise. And, since the driving motor has a high usage frequency and generates a large amount of heat, therefore, the driving motor being suspended also increases the space around it, which is beneficial for heat dissipation.

[0037] Furthermore, the microfluidic detection system further includes a position sensor for detecting the position of the slider of the sample liquid driving device. Since the slider and the piston move synchronously, the position of the piston in the syringe can be judged by the position of the slider, so as to facilitate the precise control of the displacement amount of the piston moving upward and / or downward, and achieve the precise control of sample injection.

[0038] Those skilled in the art will become more clearly aware of the above and other objects, advantages and features of the present invention according to the following detailed description of the specific embodiments of the present invention in conjunction with the accompanying drawings. Description of the Drawings

[0039] Some specific embodiments of the present invention will be described in detail hereinafter by way of example and not limitation with reference to the accompanying drawings. The same reference numerals in the drawings denote the same or similar components or parts. Those skilled in the art should understand that these drawings are not necessarily drawn to scale. In the drawings:

[0040] Figure 1 is a schematic structural diagram of a microfluidic detection system for a refrigerator according to an embodiment of the present invention;

[0041] Figure 2 is a schematic exploded view of the structure of a microfluidic detection system for a refrigerator according to an embodiment of the present invention;

[0042] Figure 3 is a schematic structural diagram of the internal structure of a microfluidic detection system according to an embodiment of the present invention;

[0043] Figure 4 is a schematic exploded view of the internal structure of a microfluidic detection system according to an embodiment of the present invention; [[ID=,18]]

[0044] Figure 5 is a schematic structural diagram of a microfluidic biochip according to an embodiment of the present invention;

[0045] Figure 6 is a schematic exploded view of a sample liquid driving device, a microfluidic biochip and related connection structures according to an embodiment of the present invention;

[0046] Figure 7 is a schematic cross-sectional view of a sample liquid driving device and a microfluidic biochip and their connection structure according to an embodiment of the present invention;

[0047] Figure 8 is a schematic cross-sectional view of a microfluidic biochip according to another embodiment of the present invention;

[0048] Figure 9 is a schematic structural diagram of a lifting mechanism and a sample stage in a disassembled state according to an embodiment of the present invention; Figure 10 is a schematic structural diagram of a refrigerator according to an embodiment of the present invention;

[0049] Figure 11 is a schematic exploded view of a door body according to an embodiment of the present invention. Detailed embodiments

[0050] The present invention first provides a microfluidic detection system for a refrigerator. The microfluidic detection system of the present invention is used for qualitatively or quantitatively detecting preset detection parameters of a sample liquid. The preset detection parameters may be, for example, pesticide residue parameters for indicating whether the pesticide residue exceeds the standard and / or the specific value of the pesticide residue amount, nutrient parameters for indicating whether the nutrient elements meet the standard and / or the specific content of the nutrient elements, specific substance parameters for indicating whether a specific harmful substance (such as a specific virus) exceeds the standard and / or the specific content, and so on.

[0051] Figure 1 FIG. 4 is a schematic structural diagram of a microfluidic detection system for a refrigerator according to an embodiment of the present invention. Figure 2 FIG. 5 is a schematic structural exploded view of a microfluidic detection system for a refrigerator according to an embodiment of the present invention. Figure 3 FIG. 6 is a schematic structural diagram of the internal structure of a microfluidic detection system according to an embodiment of the present invention. Figure 4 FIG. 7 is a schematic structural exploded view of the internal structure of a microfluidic detection system according to an embodiment of the present invention. For ease of understanding, Figures 1 to 4 the sample cup 2 is also shown in FIG. 8.

[0052] Referring to Figures 1 to 4 , the microfluidic detection system 1 involved in the present invention includes a microfluidic biochip 10, a sample liquid driving device 40, and a detection mechanism 20. Those skilled in the art can understand that when the preset detection parameters for which the microfluidic detection system is used are different, the specific selections of the microfluidic biochip 10 and the detection mechanism 20 used may also be different. For example, when the microfluidic detection system is used for pesticide residue detection, the microfluidic biochip 10 it has may be a microfluidic pesticide residue detection chip that can provide detection conditions for the pesticide residue liquid, and the detection mechanism 20 it has may be a pesticide residue detection mechanism that can detect the pesticide residue parameters of the pesticide residue liquid.

[0053] Figure 5 FIG. 9 is a schematic structural diagram of a microfluidic biochip according to an embodiment of the present invention. Referring to Figure 5 , the microfluidic biochip 10 is placed vertically and has a sample injection port 111, a communication port 112, and a detection pool 121 formed inside it. The sample injection port 111 is located at the bottom of the microfluidic biochip 10. The sample injection port 111, the detection pool 121, and the communication port 112 are sequentially connected through microchannels to form a main channel. The microchannels involved in the present invention refer to fine channels or capillary channels with a cross-sectional area within a preset size range so that they have an appropriate ability to hold the liquid inside.

[0054] The sample liquid driving device 40 is adjacently arranged on the lateral side of the microfluidic biochip 10 in the horizontal direction and is hermetically connected to the communication port 112 to urge the sample liquid in contact with the sample injection port 111 to flow through the microchannels to the detection pool 121.

[0055] The detection mechanism 20 is used to detect the detection cell 121 to obtain the preset detection parameters of the sample liquid. Specifically, a detection reagent can be preset in the detection cell 121 in advance, or the detection reagent can be added into the detection cell 121 manually or automatically, so that after the sample liquid in the detection cell 121 reacts with the detection reagent therein, the detection mechanism 20 detects the detection cell 121.

[0056] In the microfluidic detection system 1 of the present invention, the microfluidic biochip 10 is designed to be placed vertically, and its sample inlet 111 is located at its bottom, and the sample liquid driving device 40 is disposed adjacent to the lateral side of the microfluidic biochip 10 in the horizontal direction. Thus, not only the compactness of the structural layout between the microfluidic biochip 10 and the sample liquid driving device 40 is ensured, but also when the liquid in the microfluidic biochip 10 leaks or drains out, the liquid can flow downward or drip along the microfluidic biochip 10 without contacting the sample liquid driving device 40, thereby avoiding adverse effects on the sample liquid driving device 40.

[0057] Specifically, in Figures 2 to 4 the illustrated embodiment, the sample liquid driving device 40 can be adjacent to the upper right side of the microfluidic biochip 10, so as to further prevent the liquid dripping from the microfluidic biochip 10 from splashing onto the sample liquid driving device 40.

[0058] Figure 6 FIG. is a schematic exploded view of a sample liquid driving device, a microfluidic biochip and related connection structures according to an embodiment of the present invention, Figure 7 FIG. is a schematic cross-sectional view of a sample liquid driving device and a microfluidic biochip and their connection structure according to an embodiment of the present invention. In some embodiments, the sample liquid driving device 40 includes a driving motor 41. The driving motor 41 is suspended, and the top of the driving motor 41 is fixed on a bracket 87 to support the driving motor 41 through the bracket 87. That is to say, the driving motor 41 is supported by the bracket 87 fixedly connected to its top, and the driving motor 41 does not contact other structures, avoiding the vibration generated during the operation of the driving motor 41 from being transmitted to the microfluidic biochip 10 or other structures. This not only prevents adverse effects on the stability or performance of the microfluidic biochip 10 or other structures, but also reduces the vibration of the entire microfluidic detection system 1 and its operating noise. And, since the driving motor 41 has a high usage frequency and generates a large amount of heat, the driving motor 41 being suspended also increases the space around it, which is beneficial to heat dissipation.

[0059] Specifically, the driving motor 41 can be fixedly connected to the bracket 87 through the reduction gearbox part at its top, and the entire motor part is suspended, which can effectively avoid vibration transmission.

[0060] In some embodiments, the sample liquid driving device 40 can form a negative pressure in the main channel by pumping air outwards, so that the sample liquid in contact with the sampling port 111 enters the main channel under the action of the negative pressure. Specifically, the sample liquid driving device 40 can be a micro-injection pump, and further includes a vertically extending syringe 42, a screw rod 43, a slider 44 and a piston 45.

[0061] The syringe 42 is fixed on the bracket 87, and the top of the syringe 42 is hermetically connected to the communication port 112 at the top of the microfluidic biochip 10 through a connecting pipeline 46. Specifically, the syringe 42 can be located at the front side of the bracket 87, and a fixing piece 47 is provided on the front side of the syringe 42. The fixing piece 47 is fixed to the bracket 87 backwards, so as to firmly limit the syringe 42 between the fixing piece 47 and the bracket 87.

[0062] The screw rod 43 extends vertically and is connected to the driving motor 41 to rotate under the drive of the driving motor 41.

[0063] The slider 44 is sleeved on the screw rod 43 and is threadedly connected to the screw rod 43 to move up and down along the screw rod 43 as the screw rod 43 rotates. Specifically, a vertically extending guiding groove can be formed on the bracket 87, and the slider 44 is located in the guiding groove to guide the movement of the slider 44 in the up and down directions through the guiding groove.

[0064] The piston 45 is arranged inside the syringe 42 and is fixedly connected to the slider 44 to move up and down under the drive of the slider 44. Thus, when it moves downwards, a negative pressure is generated in the main channel to promote the sample liquid in contact with the sampling port 111 to flow into the microchannel and then flow into the detection pool 121 through the microchannel. When it moves upwards, it promotes the sample liquid in the main channel to flow towards the sampling port 111.

[0065] Since the main channel is connected vertically and the main channel is connected to the sample liquid driving device 40 through the connection port 112, if the sample liquid driving device 40 is not properly controlled, the sample liquid may enter the sample liquid driving device 40 through the connection port 112, thereby contaminating, corroding or damaging the sample liquid driving device 40. That is to say, during the operation of the sample liquid driving device 40, the movement amount of the piston 45 is very crucial. For this reason, in some embodiments, the sample liquid driving device 40 further includes a position sensor 48, and the position sensor 48 is used to detect the position of the slider 44 to control the operation of the driving motor 41 through the position of the slider 44, so as to control the displacement amount of the piston 45 moving upward and / or downward. Since the slider 44 and the piston 45 move synchronously, the position of the piston 45 in the syringe 42 can be judged through the position of the slider 44, so as to facilitate the precise control of the displacement amount of the piston 45 moving upward and / or downward, and further achieve the precise control of sample injection. At the same time, the displacement amount of the piston 45 moving upward and downward can also be monitored in real time, and it can perform fine pushing and sucking operations, so as to perform pushing and sucking actions in the reverse direction on the sample liquid entering the main channel on the premise of ensuring that the sample liquid does not flow out through the connection port 112, so as to make the sample liquid in the detection pool 121 and the detection reagent in it mix more evenly or react more fully, improving the accuracy of the detection result. In addition, it can also be detected by the position sensor 48 whether the piston 45 moves to the upper limit position, and when it moves to the upper limit position, the driving motor 41 is prompted to stop running to avoid the driving motor from stalling and heating up.

[0066] In some alternative embodiments, the sample liquid driving device 40 may also be other suitable types of driving devices.

[0067] In some embodiments, a reaction pool 122 for reacting the sample liquid and the reaction reagent is further formed inside the microfluidic biochip 10. The reaction pool 122 is located on the main channel and is connected between the sample injection port 111 and the detection pool 121, so that the sample liquid reacts with the reaction reagent in the reaction pool 122 first and then flows into the detection pool 121. That is to say, in some embodiments, the main channel further includes a reaction pool 122, and both between the reaction pool 122 and the sample injection port 111 and between the reaction pool 122 and the detection pool 121 are connected through microchannels.

[0068] For a specific sample solution or for some specific detection parameters of the sample solution, it may be necessary to first make the sample solution react with a reaction reagent and then react with a detection reagent. The testing agency tests the finally reacted solution to obtain the preset detection parameters of the specific sample solution, which can avoid reactions or mutual influences between the reaction reagent and the detection reagent and improve the accuracy of the test results. For example, when it is necessary to detect the pesticide residue parameters of a sample solution using the microfluidic detection system 1, the enzyme inhibition rate method is preferably used because it qualitatively detects the pesticide residue content and has a relatively fast detection speed, making it more suitable for household use. At this time, the reaction reagent and the detection reagent for the microfluidic biochip 10 can be an enzyme reagent and a chromogenic agent respectively. The reaction pool 122 is used for the reaction of the sample solution and the enzyme reagent therein. The sample solution after reacting with the enzyme reagent flows into the detection pool 121 and reacts with the chromogenic agent in the detection pool 121. At this time, the testing agency 20 can be selected as an optoelectronic detection agency, which can include a light source 21 and a photosensitive element 22 that are respectively arranged on two opposite sides of the microfluidic biochip 10 and are both facing the detection pool 121. The light emitted by the light source 21 irradiates the detection pool 121, and the light passing through the detection pool 121 is introduced into the photosensitive element 22, so as to facilitate judging the change in absorbance in the detection pool 121 through the light intensity signal received by the photosensitive element 22, and then calculating the pesticide residue inhibition rate. Further, the testing agency 20 further includes a heating sheet 24 for providing heat to the detection pool 121 and a temperature controller 25 for controlling the heating power of the heating sheet 24 to be constant, so that the sample solution and the detection reagent in the detection pool 121 react fully and quickly.

[0069] Since the purpose of setting the reaction pool 122 and the detection pool 121 in the microfluidic biochip 10 is to facilitate the reaction of the sample solution with the reaction reagent in the reaction pool 122 first and then flow to the detection pool 121, therefore, the control of the flow rate and flow volume of the sample solution in the main channel is crucial. For this reason, in some embodiments, the microchannel 141 connected between the communication port 112 and the detection pool 121 includes a bent section 1411 that extends in an S-shaped meandering manner, and the microchannel 142 connected between the detection pool 121 and the reaction pool 122 includes a bent section 1421 that extends in an S-shaped meandering manner, so as to extend the lengths of the microchannel 141 and the microchannel 142, thereby extending the flow path of the sample solution flowing from the detection pool 121 to the reaction pool 122 and the flow path of the sample solution flowing from the reaction pool 122 to the communication port 112, avoiding problems such as the sample solution flowing directly to the detection pool 121 after flowing into the main channel through the sampling port 111, resulting in inability to detect, or the sample solution flowing directly to the communication port 112 after flowing into the main channel through the sampling port 111, resulting in inability to detect and contamination, and facilitating the control of the sample solution driving device 40.

[0070] Further, in one embodiment, refer to Figure 5, the microchannel 142 connected between the detection cell 121 and the reaction cell 122 communicates with the upper end of the reaction cell 122, and the microchannel 143 connected between the reaction cell 122 and the sample inlet 111 communicates with the lower end of the reaction cell 122 and extends in the vertical direction. That is to say, the microchannel sections adjacent to the reaction cell 121 are all straight sections extending in the vertical direction, which facilitates the sample liquid to enter the reaction cell 121 under the action of a small pressure.

[0071] Figure 8 is a schematic cross-sectional view of a microfluidic biochip according to another embodiment of the present invention. When the microfluidic biochip 10 is placed vertically, the liquid in its main channel is likely to flow downward under the action of its own gravity, thereby dripping from the sample inlet 111 and affecting the normal progress of the detection. For this reason, in another embodiment, the microchannel 142 connected between the detection cell 121 and the reaction cell 122 communicates with the lower end of the reaction cell 122, and the microchannel 143 connected between the reaction cell 122 and the sample inlet 111 communicates with the upper end of the reaction cell 122, so that the microchannel 142 connected between the detection cell 121 and the reaction cell 122 further includes a bent section 1422 located between its curved section 1421 and the reaction cell 122, and the microchannel 143 connected between the reaction cell 122 and the sample inlet 111 has a bent section 1431. Thus, the curved trend of the microchannels on both sides of the reaction cell 122 is increased, preventing the sample liquid from flowing out through the sample inlet 111 under the action of its own gravity. Further, in this embodiment, the size of the reaction cell 122 can be smaller than Figure 5 the size of the reaction cell 122 in the embodiment shown, so that the sample liquid and the reaction reagent in the reaction cell 122 are mixed more evenly, thereby making the reaction between the two more sufficient and more rapid, and improving the accuracy of the detection result.

[0072] In some embodiments, the communication port 112 of the microfluidic biochip 10 is in sealed communication with the sample liquid driving device 40 through a sealing docking mechanism 90. The sealing docking mechanism 90 may include a sealing connector 91 and an elastic pressing member 92. The sealing connector 91 is connected between the microfluidic biochip 10 and the sample liquid driving device 40, and a connection channel penetrating the sealing connector 91 is formed therein. The elastic pressing member 92 is used to apply an elastic force to the sealing connector 91 so that the sealing connector 91 is in sealed docking with both the sample liquid driving device 40 and the microfluidic biochip 10 simultaneously, thereby enabling the communication port 112 of the sample liquid driving device 40 and the microfluidic biochip 10 to be in sealed communication through the connection channel inside the sealing connector 91. Thus, an elastic force can be applied to the sealing connector 91 through the elastic pressing member 92 to prompt the sealing connector 91 to always maintain a state of being in tight sealed docking with both the sample liquid driving device 40 and the microfluidic biochip 10, avoiding problems such as loosening and breakage that may occur after long-term use when using other docking mechanisms, thereby ensuring a long-term and reliable fluid sealing communication relationship between the communication port 112 of the sample liquid driving device 40 and the microfluidic biochip 10 and improving the sealing effect therebetween.

[0073] In some embodiments, the elastic pressing member 92 is disposed between the sealing connector 91 and the sample liquid driving device 40 to apply an elastic force towards the microfluidic biochip 10 to the sealing connector 91, thereby using this elastic force to prompt the sealing connector 91 to be in tight elastic contact with the microfluidic biochip 10 and using the reaction force of the microfluidic biochip 10 on the sealing connector 91 to prompt the sealing connector 91 to be in elastic sealed docking with the sample liquid driving device 40. It can be understood that in some alternative embodiments, the elastic pressing member 92 may also be disposed between the sealing connector 91 and the microfluidic biochip 10, and the principle of its action is the same as that of the above embodiments and will not be elaborated here.

[0074] In some embodiments, the sample liquid driving device 40 is in communication with the communication port 112 through a connecting pipeline 46. The communication port 112 may be formed at the top of the microfluidic biochip 10, and the sample liquid driving device 40 may be disposed adjacent to the lateral side of the microfluidic biochip 10 in the horizontal direction to avoid the adverse effects caused by possible liquid leakage of the microfluidic biochip 10 on the sample liquid driving device 40. The connecting pipeline 46 may be in communication with the top of the sample liquid driving device 40 to bridge between the sample liquid driving device 40 and the microfluidic biochip 10.

[0075] Further, the sealing connector 91 may include a first connection block 912 for directly docking with the microfluidic biochip 10 and a second connection block 913 provided on the side of the first connection block 912 facing away from the microfluidic biochip 10. A sealed connection is formed between the first connection block 912 and the second connection block 913, and between the second connection block 913 and the connection pipeline 46 in a plug-in manner.

[0076] In some embodiments, the elastic pressing member 92 may be a spring. One end of the spring abuts against a fixedly arranged end plate 513, and the other end abuts against the sealing connector 91. The end plate 513 and the microfluidic biochip 10 are respectively on opposite sides of the sealing connector 91. Specifically, when the microfluidic biochip 10 is in the installed state, the spring is in a compressed state, thereby generating an elastic force for urging the sealing connector 91 to have a tendency to move towards the microfluidic biochip 10. The number of the elastic pressing members 92 may be two or more to increase the magnitude of the elastic force acting on the microfluidic biochip 10 and make the elastic force received by the microfluidic biochip 10 more balanced, avoiding tilting, and further improving the sealing connection effect.

[0077] Further, the sealing and docking mechanism 90 further includes a guide rod 93. The spring is sleeved on the guide rod 93 to prevent the spring from shifting. One end of the guide rod 93 is fixedly connected to the sealing connector 91, and the other end contacts a Hall switch 94 after the microfluidic biochip 10 and the sealing connector 91 are sealed and docked, thereby prompting the Hall switch 94 to generate a trigger signal indicating that the microfluidic biochip 10 is installed in place, so as to prompt the user, avoiding structural damage caused by over-installation of the microfluidic biochip 10, and at the same time improving the user experience.

[0078] In some embodiments, the microfluidic detection system 1 further includes a clamping mechanism 51. The clamping mechanism 51 is used to clamp the microfluidic biochip 10 to keep it in a fluid-tight connection state with the sample liquid driving device 40. The direction of the force generated by the clamping mechanism 51 on the microfluidic biochip 10 is perpendicular to the direction of the force generated by the sealing and docking mechanism 90 on the microfluidic biochip 10. Thus, the microfluidic biochip is fixed and supported in two different directions, improving the stability of the installation of the microfluidic biochip 10.

[0079] Further, the clamping mechanism 51 may include two relatively arranged elastic jaws 511 to apply an opposing force to the microfluidic biochip 10 clamped between the two elastic jaws 511.

[0080] Since the microfluidic detection system 1 is used to be integrated on a refrigerator and the operating space is small, the disassembly operation of the microfluidic biochip 10 cannot basically act directly on the chip installation mechanism 51.

[0081] For this reason, in some embodiments, the microfluidic detection system 1 of the present application is specifically designed with a chip ejection mechanism 52. The chip ejection mechanism 52 is configured to operably apply opposite-direction forces to the two elastic jaws 511, so as to cause the two elastic jaws 511 to elastically deform in a direction away from each other, thereby releasing the clamping effect of the two elastic jaws 511 on the microfluidic biochip 10. That is to say, the user can apply a separating force to the two elastic jaws 511 through the chip ejection mechanism 52, causing the two elastic jaws 511 to elastically deform in a direction away from each other, thereby releasing the clamping effect of the two elastic jaws 511 on the microfluidic biochip 10 and releasing the microfluidic biochip 10, and the microfluidic biochip 10 can be easily disassembled. Moreover, since the chip ejection mechanism 52 is exposed outside, it is convenient for the user to perform the chip ejection operation. No matter how compact the structural layout of the microfluidic detection system 1 itself and its overall structural layout after being integrated into the refrigerator are, it will not affect the disassembly operation of the microfluidic biochip 10, improving the user experience. Preferably, the chip ejection mechanism 52 can be exposed outside the microfluidic detection system 1 to facilitate the user to perform the chip ejection operation.

[0082] In some embodiments, the chip ejection mechanism 52 may include a cantilever button hanging on one side of the microfluidic biochip 10. The cantilever button is simultaneously in contact with the inner sides of the two elastic jaws 511 arranged facing each other, so as to apply an outward force to the inner sides of the two elastic jaws 511 when the cantilever button is subjected to a force towards the microfluidic biochip 10, thereby causing the two elastic jaws 511 to elastically deform in an outward direction away from each other. That is to say, when it is necessary to disassemble the microfluidic biochip 10, the user only needs to press the cantilever button to release the clamping effect of the two elastic jaws 511 on the microfluidic biochip 10, thereby releasing the microfluidic biochip 10. The operation is very simple, and the structure of the chip ejection mechanism 52 is very simple and the design is very ingenious.

[0083] In some embodiments, the microfluidic detection system 1 further includes a sample stage 70 disposed below the microfluidic biochip 10 for placing a sample cup 2 which is used to hold a sample liquid. Moreover, the sample stage 70 is configured to move controllably or operably so as to convey the sample cup 2 placed thereon to a position where the sample liquid in the sample cup 2 is allowed to contact the sample inlet 111 of the microfluidic biochip 10. Thereby, sample loading of the microfluidic biochip 10 is achieved. The user only needs to place the sample cup 2 on the sample stage 70, or after placing the sample cup 2 on the sample stage 70, move the sample stage 70 to a position where it contacts the sample inlet 111 of the microfluidic biochip 10. The sample loading operation is very convenient, saving time and effort. Moreover, in this application, by configuring the sample stage 70 to be movable, complex structures such as a sample liquid delivery pump, delivery pipelines, sampling needles, etc. are eliminated, making the structure of the microfluidic detection system 1 very simple, so that it is suitable for integration on a refrigerator for convenient home use.

[0084] Furthermore, the microfluidic detection system 1 further includes a lifting mechanism 60 for driving the sample stage 70 to move up and down so that the sample stage 70 can switch between a detection position where the sample liquid in the sample cup 2 placed on the sample stage 70 is allowed to contact the sample inlet 111 and an initial position which is at a preset distance below the detection position. That is to say, the sample stage 70 can be automatically lifted and lowered by the lifting mechanism 60.

[0085] Figure 9 It is a schematic structural diagram of a lifting mechanism and a sample stage in a disassembled state according to an embodiment of the present invention. In some embodiments, the lifting mechanism 60 may include a lifting motor 61, a transmission screw rod 62 and a nut 63. The lifting motor 61 is used to output a driving force. The transmission screw rod 62 is disposed in the vertical direction and is connected to the output shaft of the lifting motor 61 to rotate under the drive of the lifting motor 61. The nut 63 is sleeved on the transmission screw rod 62 and is threadedly connected to the transmission screw rod 62 to move up and down along the transmission screw rod 62 as the transmission screw rod 62 rotates. The sample stage 70 is fixedly connected to the nut 63 to drive the sample stage 70 to move up and down through the nut 63.

[0086] Furthermore, the lifting mechanism 60 also includes a slide rail 64 and a slider 65. The slide rail 64 is arranged parallel to the transmission screw 62 and is arranged beside the transmission screw 62. The slider 65 is movably arranged on the slide rail 64. The sample stage 70 is fixedly connected to the slider 65 so as to guide the sample stage 70 to move up and down through the cooperation of the slide rail 64 and the slider 65. Specifically, when the sample stage 70 moves in the up and down directions under the action of the drive module, it drives the slider 65 to move synchronously. The slider 65 is restricted on the slide rail 64. The slide rail 64 has a guiding and limiting effect on the movement of the slider 65, thereby indirectly guiding and limiting the sample stage 70, avoiding the sample stage 70 from deflecting or jamming during movement, and improving the smoothness of the movement of the sample stage 70. Specifically, the sample stage 70 may include a horizontal connecting plate 74 that passes through the transmission screw 62 and is fixedly connected to the nut 63, and a vertical connecting plate 75 that extends upward perpendicular to the horizontal connecting plate 74. The vertical connecting plate 75 is fixedly connected to the slider 65.

[0087] In some embodiments, the lifting mechanism 60 further includes a limit switch 66, which is disposed adjacent to the upper portion of the transmission screw 62 to cause the lifting motor 61 to stop running when the sample stage 70 moves upward and touches the limit switch 66. Furthermore, the position of the limit switch 66 is configured so that when the lifting motor 61 stops running under the triggering of the limit switch 66, the sample stage 70 is in its detection position. The lifting motor 61 can maintain the sample stage 70 in its detection position when it is not running. The present application uses the limit switch 66 to locate the detection position of the sample stage 70, and the positioning is precise, which can avoid the problem that the sample stage 70 continues to move beyond its detection position, causing damage to the sample stage 70, the microfluidic biochip 10, and other structures.

[0088] In some embodiments, the sample stage 70 may include a support platform 71 and an oscillator 72. The support platform 71 is used to support the sample cup 2. Specifically, the support platform 71 may be a horizontal support plate. The support plate may be provided with a groove for the bottom of the sample cup 2 to rest within. This prevents the sample cup 2 from tipping or shaking during movement of the sample stage 70, thereby improving the stability of the sample cup 2. The support platform 71 is fixedly connected to a horizontal connecting plate 74.

[0089] An oscillator 72 is mounted on the support 71 and is used to oscillate the sample cup 2 after it is placed on the support 71. This allows the buffer solution in the sample cup 2 to mix thoroughly with the sample, thereby producing a sample solution. This allows the substance to be detected in the sample to be fully dissolved in the buffer solution, resulting in a sample solution of appropriate concentration. The buffer solution can be manually added to the sample cup 2 or automatically delivered to the sample cup 2 by a drive device after the sample cup 2 is placed on the sample stage 70.

[0090] In some embodiments, the sample stage 70 further includes a load cell 73 disposed below the support stage 71 for weighing the sample in the sample cup 2, so that the buffer driving device 30 is allowed to deliver a preset amount of buffer matching the weight of the sample to the sample cup 2. Generally, household users extract samples rather casually, such as randomly tearing off a small piece of leaf. Therefore, to ensure the accuracy of the measurement results, the amount of buffer input into the sample cup 2 needs to match the amount of the sample, so as to produce a sample solution with an appropriate concentration. Through the load cell 73 disposed below the support stage 71, the present application can automatically and accurately obtain the weight of the sample, thereby automatically controlling the buffer driving device 30 to input a matching amount of buffer into the sample cup 2, which not only ensures the accuracy of the measurement results, but also avoids many problems such as inconvenient use, cumbersome operation, and large errors caused by the user manually weighing the sample, and further improves the automation degree of the microfluidic detection system and the user experience.

[0091] It should be noted that in some alternative embodiments, the sample stage 70 can be fixed and the microfluidic biochip 10 can be set to be movable, which can also facilitate the sampling operation.

[0092] In some embodiments, the microfluidic detection system 1 further includes a housing 80. An operation table 83 that is open towards the front side is formed on the housing 80, and the sample stage 70 is at least partially located in the operation table 83, so as to facilitate the user to perform operations such as placing the sample cup 2 and taking out the sample cup 2 in the operation table 83. A water receiving box 88 can be provided in the operation table 83 below the sample stage 70 to receive the liquid that may drip and avoid contaminating the operation table 83. At least a part of the microfluidic biochip 10, the detection mechanism 20, the buffer bottle 36, and the buffer driving device 30 are all provided inside the housing 80. Further, the housing 80 is provided with a first structural connector 81 for connecting to the box body or the door body of the refrigerator, and a first electrical connector 82 for forming an electrical connection between the microfluidic detection system 1 and the electrical control device of the refrigerator 100, so as to allow the microfluidic detection system 1 to be installed as a whole on the box body or the door body of the refrigerator.

[0093] In some embodiments, the microfluidic biochip 10 is removably positioned above the sample stage 70, and the injection port 111 is located at the bottom of the microfluidic biochip 10. Moreover, the microfluidic detection system 1 further includes a chip mounting mechanism 51 and a chip ejection mechanism 52. The chip mounting mechanism 51 is disposed within the housing 80 and is configured to support the microfluidic biochip 10. The chip ejection mechanism 52 is exposed on the front side of the housing 80 and is configured to operably release the support of the chip mounting mechanism 51 on the microfluidic biochip 10, so as to release the microfluidic biochip 10, causing it to fall onto the sample stage 70 under its own gravity. When the sample cup 2 is placed on the sample stage 70, the microfluidic biochip 10 can automatically fall into the sample cup 2, facilitating its removal and discard together with the sample cup 2.

[0094] In some embodiments, the microfluidic detection system 1 further includes a buffer solution bottle 36 and a buffer solution driving device 30. The buffer solution bottle 36 is disposed within the housing 80 and is configured to contain the buffer solution. The buffer solution driving device 30 is disposed within the housing 80 and is connected to the buffer solution bottle 36 to controllably drive the buffer solution in the buffer solution bottle 36 into the sample cup 2 placed on the sample stage 70, so that the buffer solution is mixed with the sample in the sample cup 2 to generate a sample solution. Specifically, the buffer solution bottle 36 and the buffer solution driving device 30 are connected through an introduction tube 32. The outlet tube 31 of the buffer solution driving device 30 extends to the sample stage 70. This is mainly for the case where the detected sample is a solid sample, and the buffer solution is needed to dissolve the substance to be detected on the solid sample to form a sample solution; or, the sample is a liquid sample, but its concentration is too high, and the buffer solution is needed to dilute it to generate a sample solution. For example, when performing pesticide residue detection, the detected sample is usually solid food residues such as epidermis and leaves, and the sample needs to be placed in the buffer solution, and the residual pesticides on the sample are dissolved in the buffer solution to form a sample solution.

[0095] Specifically, the buffer solution driving device 30 can be a peristaltic pump, a diaphragm pump or other suitable types of driving devices. When a peristaltic pump or a diaphragm pump is operating, it will generate relatively large vibrations in its radial direction. To prevent this vibration from being transmitted to the microfluidic biochip 10, an elastic vibration damping member 35 can be provided on the outer side in the radial direction of the peristaltic pump or the diaphragm pump. The elastic vibration damping member 35 can be sleeved outside the buffer solution driving device 30 and is supported within the housing 80 by the clamping action of the bracket 87 and the fixing block 89, and the fixing block 89 can be fixed on the support plate 86.

[0096] In some embodiments, the microfluidic detection system 1 further includes a circuit board 53, a display device 56 and a switch button 57. The circuit board 53 is disposed in the housing 80 and is electrically connected to the first electrical connector 82 on the housing 80. The electrical components of the microfluidic detection system 1 (such as the lifting mechanism 60, the buffer drive device 30, the sample liquid drive device 40, the display device 56, the switch button 57, etc.) are all electrically connected to the circuit board 53 directly or indirectly. The display device 56 is disposed on the front side of the housing 80 and is electrically connected to the circuit board 53 for displaying the detection results of the detection mechanism 20. The switch button 57 is disposed on the front side of the housing 80 and is electrically connected to the circuit board 53 for starting and / or shutting down the detection function of the microfluidic detection system 1. In other words, the user can start, pause or stop the detection function of the microfluidic detection system 1 by operating the switch button 57.

[0097] In some embodiments, the housing 80 may include a rear shell 84 at the rear side and a front panel 85 connected to the front side of the rear shell 84. When assembled, the rear shell 84 and the front panel 85 define a storage chamber therebetween. Furthermore, a support plate 86 and a bracket 87 are provided within the storage chamber of the housing 80. The support plate 86 is fixedly connected to the rear shell 84, and at least a portion of the structure of the lifting mechanism 60 (such as the non-movable portion of the lifting mechanism) and the buffer drive device 30 are fixed to the support plate 86. The bracket 87 is fixedly connected to the front side of the support plate 86, and the microfluidic biochip 10 and the sample liquid drive device 40 are directly or indirectly supported by the bracket 87. Thus, the support plate 86 and the bracket 87 can stably support the lifting mechanism 60, the buffer drive device 30, the microfluidic biochip 10, and the sample liquid drive device 40 within the storage chamber formed between the rear shell 84 and the front panel 85.

[0098] In some embodiments, the lifting mechanism 60 can be positioned laterally to the side of the sample stage 70, the buffer drive device 30 can be positioned on one side of the microfluidic biochip 10 and above the lifting mechanism 60, the sample liquid drive device 40 is positioned on the other side of the microfluidic biochip 10, and the buffer bottle 36 is positioned on the side of the sample liquid drive device 40 facing away from the microfluidic biochip 10. This arrangement of the microfluidic biochip 10, sample stage 70, lifting mechanism 60, buffer drive device 30, sample liquid drive device 40, and buffer bottle 36 fully utilizes the vertical and lateral dimensions of each module, making the layout of each module more compact and minimizing the space occupied. Furthermore, the modules are arranged side by side only in the vertical and lateral directions, minimizing the thickness of the microfluidic detection system 1 in the front-to-back direction, making it more suitable for integration into a refrigerator.

[0099] Further, a laterally extending partition 861 may be provided between the buffer driving device 30 and the lifting mechanism 60 to prevent the leakage of the buffer solution that may occur in the buffer driving device 30 from dripping onto the lifting mechanism 60 and affecting the normal operation of the lifting mechanism 60. The partition 861 may be fixed to the support plate 86.

[0100] The present invention also provides a refrigerator, Figure 10 which is a schematic structural diagram of a refrigerator according to an embodiment of the present invention. The refrigerator 100 of the present invention includes the microfluidic detection system 1 involved in any of the above embodiments, so as to integrate the microfluidic detection system 1 on the refrigerator 100. The refrigerator 100 has a high usage frequency in daily life, and the refrigerator 100 is mainly used to store food materials. After integrating the microfluidic detection system 1 on the refrigerator 100, it is convenient for users to use the microfluidic detection system 1 to perform the detection operation of food material samples.

[0101] Further, the refrigerator 100 further includes a box body 200 and a door body 300. A storage space is defined in the box body 200. The door body 300 is connected to the box body 200 and is used to open and / or close the storage space. The microfluidic detection system 1 is preferably arranged on the door body 300, which is not only convenient to operate, but also does not occupy the original storage space in the box body 200 and does not affect the storage capacity of the refrigerator 100 itself.

[0102] Figure 11 which is a schematic exploded structural diagram of a door body according to an embodiment of the present invention. In some embodiments, a hollow window 301 is provided on the front side of the door body 300. The sample stage 70 of the microfluidic detection system 1 is exposed to the front side of the door body 300 through the hollow window 301. Thus, without opening the door body 300, users can place a sample cup on the sample stage 70, avoiding the problem of serious cold leakage caused by opening the door body 300 every time a detection is performed, ensuring the heat preservation performance of the refrigerator 100 and saving energy consumption.

[0103] Specifically, the door body 300 may include a panel 302 for forming its front part, a door liner 303 for forming its rear part, and a foaming heat insulation layer (not shown in the figure) provided between the panel 302 and the door liner 303. The hollow window 301 is opened on the panel 302. A pre-embedded box 304 is pre-embedded between the panel 302 and the door liner 303 before forming the foaming heat insulation layer. The microfluidic detection system 1 is arranged in the pre-embedded box 304. That is to say, the pre-embedded box 304 is pre-set between the panel 302 and the door liner 303 before the door body 300 is foamed, and is used to reserve a space for installing the microfluidic detection system 1 between the panel 302 and the door liner 303.

[0104] Furthermore, the embedded box 304 is attached to the rear surface of the panel 302, and the front side of the embedded box 304 is open and faces the hollow window 301, so as to allow the microfluidic detection system 1 to be installed into the embedded box 304 from front to back through the hollow window 301, which improves the convenience of installing the microfluidic detection system 1.

[0105] Specifically, the embedded box 304 may be provided with a second structural connector 305 that is matched and connected with the first structural connector 81 and a second electrical connector 306 that is electrically connected with the first electrical connector 82, and the second electrical connector 306 is electrically connected with the electrical control device of the refrigerator 100. Thus, by providing corresponding structural connectors and electrical connectors on the embedded box 304 and the housing 80, the microfluidic detection system 1 is installed on the door body 300 as a whole, so as to realize the connection between the entire microfluidic detection system 1 and the refrigerator 100 in terms of both structure and circuit. Thus, not only the assembly process of the microfluidic detection system 1 is simplified, but also the disassembly or maintenance of the microfluidic detection system 1 is facilitated.

[0106] The refrigerator 100 in this application is a refrigerator in a broad sense, which not only includes the commonly said refrigerator in a narrow sense, but also includes storage devices with refrigeration, freezing or other storage functions, such as refrigerators, freezers, and so on.

[0107] Those skilled in the art should also understand that the terms such as "upper", "lower", "front", "rear", "top", "bottom", etc. used to represent the orientation or position relationship in the embodiments of the present invention are based on the actual use state of the microfluidic detection system 1 and the refrigerator 100. These terms are only for the convenience of describing and understanding the technical solution of the present invention, rather than indicating or implying that the device or component referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation to the present invention.

[0108] At this point, those skilled in the art should recognize that although many exemplary embodiments of the present invention have been shown and described in detail herein, still, without departing from the spirit and scope of the present invention, many other variations or modifications that conform to the principles of the present invention can be directly determined or derived based on the content disclosed in the present invention. Therefore, the scope of the present invention should be understood and recognized as covering all these other variations or modifications.

Claims

1. A microfluidic detection system for refrigerators, characterized in that: include: A microfluidic biochip is placed vertically and has an injection port, a communication port, and a detection pool formed therein, wherein the injection port is located at the bottom of the microfluidic biochip, and the injection port, the detection pool, and the communication port are sequentially connected by a microchannel to form a main channel; a sample liquid driving device, disposed adjacent to the side of the microfluidic biochip in a lateral direction and sealedly connected to the communication port, so as to cause the sample liquid in contact with the sample inlet to flow toward the detection cell through the microchannel; and A detection mechanism, used to detect the detection pool to obtain preset detection parameters of the sample liquid; A reaction pool for reacting the sample liquid and the reaction reagent is further formed inside the microfluidic biochip. The reaction pool is located on the main channel and is connected between the injection port and the detection pool, so that the sample liquid reacts with the reaction reagent in the reaction pool before flowing into the detection pool. The microfluidic channel connected between the detection pool and the reaction pool is communicated with the lower end of the reaction pool, and the microfluidic channel connected between the reaction pool and the injection port is communicated with the upper end of the reaction pool, so that the microfluidic channel connected between the detection pool and the reaction pool has a bending section whose height is lower than that of the reaction pool, and the microfluidic channel connected between the reaction pool and the injection port has a bending section whose height is higher than that of the reaction pool.

2. The microfluidic detection system according to claim 1, characterized in that: The sample liquid driving device includes a driving motor. The driving motor is suspended in the air, and the top of the driving motor is fixed on a bracket to support the driving motor.

3. The microfluidic detection system according to claim 2, characterized in that: The sample liquid driving device is a micro syringe pump, and further includes: a vertically extending syringe, the syringe being fixed to the bracket, and the top of the syringe being sealedly connected to the communication port at the top of the microfluidic biochip via a connecting pipe; a screw rod extending vertically and connected to the drive motor to rotate under the drive of the drive motor; a slider, which is passed through the screw rod and is threadably connected to the screw rod so as to translate up and down along the screw rod as the screw rod rotates; and The piston is arranged inside the syringe and is fixedly connected to the slider so as to be translated in the up and down directions under the drive of the slider, so that when it translates downward, a negative pressure is generated in the main channel, thereby prompting the sample liquid in contact with the injection port to flow into the microchannel and then into the detection cell through the microchannel, and when it translates upward, it prompts the sample liquid in the main channel to flow toward the injection port.

4. The microfluidic detection system according to claim 3, characterized in that: Also includes: The position sensor is used to detect the position of the slider, so as to control the operation of the drive motor according to the position of the slider, thereby controlling the displacement of the piston in the upward and / or downward translation.

5. The microfluidic detection system according to claim 1, characterized in that: The microfluidic channel connected between the communication port and the detection cell, and the microfluidic channel connected between the detection cell and the reaction cell both include a curved section extending in an S-shaped circuitous manner; and The microchannel connected between the detection pool and the reaction pool is communicated with the upper end of the reaction pool, and the microchannel connected between the reaction pool and the injection port is communicated with the lower end of the reaction pool and extends in a vertical direction.

6. The microfluidic detection system according to claim 1, characterized in that: The micro-channel connected between the communication port and the detection cell, and the micro-channel connected between the detection cell and the reaction cell both include a curved section extending in an S-shape.

7. The microfluidic detection system according to claim 1, characterized in that: The communication port of the microfluidic biochip is sealed and connected to the sample liquid driving device through a sealing docking mechanism, and the sealing docking mechanism includes: a sealing connector having a connection channel formed therein that passes through the sealing connector; and an elastic pressure member for applying an elastic force to the sealing connector so that the sealing connector is simultaneously sealed and docked with the sample liquid driving device and one end of the communication port of the microfluidic biochip, thereby sealingly connecting the sample liquid driving device and the communication port through the connecting channel.

8. The microfluidic detection system according to claim 1, characterized in that: Also includes: A sample stage is provided below the microfluidic biochip for placing a sample cup, wherein the sample cup is used to hold a sample liquid; The sample stage is configured to move up and down in a controlled or operable manner, so as to transport the sample cup placed thereon upward to a position where the sample liquid in the sample cup is allowed to contact the sample inlet of the microfluidic biochip.

9. The microfluidic detection system according to claim 8, characterized in that: The sample stage comprises: a support table for supporting the sample cup; and The oscillator is arranged on the supporting platform and is used to oscillate the sample cup after the sample cup is placed on the supporting platform, so that the buffer solution and the sample in the sample cup are fully mixed to produce the sample liquid.

10. A refrigerator, characterized in that: The microfluidic detection system comprises any one of claims 1-9.

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