Automated processing equipment for the vitrification of biological materials

Abstract

The present invention relates to an automated processing device for vitrification of biological materials, comprising: a frame; a processing member disposed on the frame, the processing member including a main body and an opening valve, the main body being provided with a first cavity and a second cavity, the first cavity and the second cavity being communicated through a first channel, and the first channel only allowing liquid to pass through, a second channel communicating with the second cavity and the outside is provided in the main body, the opening valve is detachably disposed in the second channel, and the opening valve can seal the second channel; a moving carrier movably disposed on the frame; a liquid injection device disposed on the moving carrier, the liquid injection device can inject or suck liquid into the first cavity driven by the moving carrier; and a first material taking member disposed on the moving carrier, the first material taking member can take out the opening valve from the second channel driven by the moving carrier. This automated processing device can stably perform cryopreservation pretreatment and transfer of biological materials, realizing standard automated operation.

Description

Technical Field

[0001] The present invention relates to the technical field of biological material processing, and particularly to an automated processing device for vitrification of biological materials. Background Art

[0002] Currently, the cryopreservation pretreatment of biological materials is usually manually completed by operators. The operating habits and skills of different operators are different, and it is impossible to guarantee the quality of the cryopreservation pretreatment of biological materials. Some current automated designs set up an operating tank with a special-shaped tank for blocking and preventing the loss of biological materials. During cryopreservation pretreatment, the biological materials are placed in the special-shaped tank, and then an operating liquid is injected into the operating tank. After cryopreservation pretreatment, the special-shaped tank containing the biological materials is sealed and directly put into liquid nitrogen. This method is not applicable to the current cryopreservation carrier rod for biological materials, and in this way, the biological materials are easily stuck in the special-shaped tank, and it is impossible to quickly and accurately take out the biological materials and transfer them to the carrier rod, resulting in the inability to standardize the automated vitrification treatment of biological materials. Summary of the Invention

[0003] Based on this, in view of the problem that the automated vitrification treatment of biological materials cannot be standardized, it is necessary to provide an automated processing device for vitrification of biological materials that can stably perform cryopreservation pretreatment and transfer of biological materials and realize standardized automated operation.

[0004] An automated processing device for vitrification of biological materials, comprising:

[0005] A frame;

[0006] A processing member disposed on the frame. The processing member includes a body and an opening valve. The body is provided with a first cavity and a second cavity, and the first cavity is communicated with the second cavity through a first channel, and the first channel only allows liquid to pass through. A second channel communicating with the second cavity and the outside is provided in the body. The opening valve is detachably disposed in the second channel, and the opening valve can seal the second channel;

[0007] A moving carrier movably disposed on the frame;

[0008] A liquid injection device disposed on the moving carrier. The liquid injection device can inject or suck liquid into the first cavity under the drive of the moving carrier; and

[0009] A first material taking member disposed on the moving carrier. The first material taking member can take out the opening valve from the second channel under the drive of the moving carrier.

[0010] The above-mentioned automated processing equipment for vitrification of biological materials can install the opening valve in the second channel and seal the second channel. The biological material is added to the second cavity. The moving carrier can drive the liquid injection device to move to the position of the first cavity, and the liquid injection device can inject the cryoprotectant into the first cavity. Due to the setting of the first channel, the cryoprotectant in the first cavity can flow into the second cavity, while the biological material in the second cavity cannot flow into the first cavity. At this time, the cryoprotectant in the second cavity will perform cryopreprocessing on the biological material. When the predetermined processing time is reached, the liquid injection device will suck out the cryoprotectant in the first cavity. It can be understood that the cryoprotectant in the second cavity will flow into the first cavity and be sucked out, completing the cryopreprocessing of the biological material. Next, the moving carrier drives the first material taking part to move to the opening valve and takes out the opening valve from the second channel. The cryopreprocessed biological material is transferred through the second channel to the cryogenic carrier rod for storage. This automated processing equipment can quickly and stably perform cryopreprocessing operations on biological materials, and can ensure the consistency of cryopreprocessing operations, without being affected by the operator's experience and skills. The cryopreprocessed biological materials can be quickly transferred and stored, effectively ensuring the quality of the obtained biological materials, avoiding the risk of damage to biological materials caused by manual processing and transfer, and realizing standard automated operations. The biological material can be a biological germ cell.

[0011] In one embodiment, a first mating part is provided at the end of the first cavity, and a second mating part is provided at the end of the second cavity. The first mating part and the second mating part are staggered in position to form the first channel. This setting method can conveniently and effectively form the first channel, and improves the technical problem of difficult processing of the first channel to meet smaller sizes.

[0012] In one embodiment, the first mating part and the second mating part are spaced apart in a direction perpendicular to the liquid flow direction, and the end face of the first mating part facing the second cavity is flush with the end face of the second mating part facing the first cavity or staggered in the direction of liquid flow.

[0013] In one embodiment, the second channel is a circular channel, and the opening valve has a cylindrical end, and the cylindrical end can be inserted into the second channel and seal the second channel.

[0014] In one embodiment, the opening valve has an opposite top end and bottom end. A first flange protrudes from the outer periphery of the top end. The first material taking part includes a material moving groove extending inward from the end. The material moving groove can allow the top end to slide in, and the top of the first material taking part abuts against the first flange. The first material taking part moves upward and can take out the opening valve; the bottom end is a cylindrical end.

[0015] In one embodiment, a second flange protrudes on the outer periphery of the cylindrical end. The second flange is annular, and the second flange is in close fit with the second channel.

[0016] In one embodiment, it further includes a driving device. The driving end of the driving device is connected to the moving carrier, and the driving device can drive the liquid injection device and the first material taking member to move horizontally, longitudinally and vertically.

[0017] In one embodiment, the moving carrier includes a first base body, a second base body and a third base body. The driving device includes a first driving member, a second driving member and a third driving member. The first base body is slidably arranged on the frame horizontally, the second base body is slidably arranged on the first base body longitudinally, the third base body is slidably arranged on the second base body vertically. The driving end of the first driving member is connected to the first base body horizontally, the driving end of the second driving member is connected to the second base body longitudinally, and the driving end of the third driving member is connected to the third base body vertically; the liquid injection device and the first material taking member are arranged on the third base body.

[0018] In one embodiment, it further includes a second material taking member and a carrier rod. The carrier rod is inserted into the frame and is located directly below the second channel; the second material taking member includes a fourth driving member, a first clamping arm and a second clamping arm. The fourth driving member is slidably arranged on the second base body vertically. The first clamping arm and the second clamping arm are spaced vertically, and the first clamping arm and the second clamping arm are connected to the driving end of the fourth driving member. The fourth driving member can drive the first clamping arm and the second clamping arm to move towards each other to clamp the carrier rod, drive the first clamping arm and the second clamping arm to move away from each other to release the carrier rod, and drive the first clamping arm and the second clamping arm to rotate to tilt the carrier rod.

[0019] In one embodiment, it includes at least one of the following technical solutions:

[0020] The liquid injection device includes a liquid injection head and a liquid suction head. The liquid injection head can inject liquid into the first cavity, and the liquid suction head can suck out the liquid in the first cavity;

[0021] It further includes a first vision detector. The processing member is a transparent member. The first vision detector is arranged on the frame and is located directly in front of the processing member. The first vision detector can detect the liquid conditions in the first cavity and the second cavity, and detect the state of the biological material in the second cavity;

[0022] It further includes a carrier rod and the second vision detector. The carrier rod is arranged directly below the second channel, and the second vision detector is arranged on the frame and can detect the situation of the biological material falling onto the carrier rod.

[0023] It further includes a pressurizing device. The pressurizing device is arranged on the mobile carrier and can pressurize the second channel along with the drive of the mobile carrier, so that the biological material in the second cavity falls from the second channel onto the carrier rod. Description of the Drawings

[0024] Figure 1 It is a schematic structural diagram of the first embodiment of the automated processing equipment of the present invention;

[0025] Figure 2 It is a schematic structural diagram of the first embodiment of the processing part of the present invention;

[0026] Figure 3 It is a schematic structural diagram of the second embodiment of the processing part of the present invention;

[0027] Figure 4 It is a partial schematic structural diagram of the first embodiment of the processing part of the present invention;

[0028] Figure 5 It is a schematic structural diagram of the first embodiment of the opening valve of the present invention;

[0029] Figure 6 It is a schematic structural diagram of the second embodiment of the automated processing equipment of the present invention;

[0030] Figure 7 It is a schematic structural diagram of the third embodiment of the automated processing equipment of the present invention;

[0031] Figure 8 It is a schematic structural diagram of the fourth embodiment of the automated processing equipment of the present invention.

[0032] In the drawings, the list of components represented by each reference numeral is as follows:

[0033] 100. Automated processing equipment for vitrification of biological materials; 1. Frame; 2. Processing part; 21. Body; 211. First cavity; 2111. Expansion part; 2112. First mating part; 212. Second cavity; 2121. Second mating part; 22. Opening valve; 221. First flange; 222. Second flange; 23. First channel; 24. Second channel; 3. Moving carrier; 31. First base; 32. Second base; 33. Third base; 4. Liquid injection device; 41. Liquid injection head; 42. Liquid suction head; 5. First material taking part; 51. Material transfer groove; 61. First driving part; 611. First motor; 612. First ball screw; 613. First guide rail; 62. Second driving part; 621. Second motor; 622. Second ball screw; 623. Second guide rail; 63. Third driving part; 631. Third motor; 632. Third ball screw; 633. Third guide rail; 7. Second material taking part; 8. Carrier rod; 9. Liquid nitrogen carrying device; 110. Fourth driving part; 120. First clamping arm; 130. Second clamping arm; 140. First vision detector; 150. Pressurizing device. Detailed implementation manners

[0034] To make the above objects, features, and advantages of the present invention more obvious and understandable, the following describes the detailed implementation manners of the present invention clearly and completely with reference to the accompanying drawings. Obviously, the specific details described below are only some embodiments of the present invention, and the present invention can also be implemented in many other embodiments different from those described herein. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0035] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are only for the purpose of illustration and do not represent the only implementation manner.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs. The terms used in the description of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0037] Please refer to Figure 1 and Figure 2, in one embodiment, an automated processing device 100 for vitrification of biological materials includes: a frame 1, a processing member 2, a moving carrier 3, a liquid injection device 4, and a first material picking member 5. The processing member 2 is disposed on the frame 1. The processing member 2 includes a main body 21 and an opening valve 22. The main body 21 is provided with a first cavity 211 and a second cavity 212. The first cavity 211 and the second cavity 212 are communicated through a first channel 23, and the first channel 23 only allows liquid to pass through. A second channel 24 communicating with the second cavity 212 and the outside is provided in the main body 21. The opening valve 22 is detachably disposed in the second channel 24, and the opening valve 22 can seal the second channel 24. The moving carrier 3 is movably disposed on the frame 1. The liquid injection device 4 is disposed on the moving carrier 3. The liquid injection device 4 can move to the position of the first cavity 211 driven by the moving carrier 3 and inject or suck liquid into the first cavity 211. The first material picking member 5 is disposed on the moving carrier 3. The first material picking member 5 can move to the position of the opening valve 22 driven by the moving carrier 3 and can take out the opening valve 22 from the second channel 24.

[0038] For the automated processing device 100 for vitrification of biological materials, the opening valve 22 can be installed in the second channel 24 and the second channel 24 can be sealed. Biological materials are added into the second cavity 212. The moving carrier 3 can drive the liquid injection device 4 to move to the position of the first cavity 211, and the liquid injection device 4 can inject cryoprotectant into the first cavity 211. Due to the setting of the first channel 23, the cryoprotectant in the first cavity 211 can flow into the second cavity 212, while the biological materials in the second cavity 212 cannot flow into the first cavity 211. At this time, the cryoprotectant in the second cavity 212 will perform cryopreprocessing on the biological materials. When the predetermined processing time is reached, the liquid injection device 4 will suck out the cryoprotectant in the first cavity 211. It can be understood that the cryoprotectant in the second cavity 212 will flow into the first cavity 211 and be sucked out, completing the cryopreprocessing of the biological materials. Next, the moving carrier 3 drives the first material picking member 5 to move to the position of the opening valve 22 and takes out the opening valve 22 from the second channel 24. The cryopreprocessed biological materials are transferred through the second channel 24 to a cryogenic carrier rod for storage. This automated processing device can perform cryopreprocessing operations on biological materials quickly and stably, and can ensure the consistency of cryopreprocessing operations, without being affected by the experience and skills of operators. The preprocessed biological materials can be quickly transferred and stored, effectively ensuring the quality of the obtained biological materials, avoiding the risk of damage to biological materials caused by manual processing and transfer of biological materials, and realizing standard automated operations. The biological materials can be biological germ cells.

[0039] The first cavity 211 and the second cavity 212 can be formed at the top of the body 21, facilitating the cryopreprocessing operation of biological materials. The shape of the first cavity 211 can be conical, square or circular.

[0040] Please refer to Figure 2 and Figure 4 In other embodiments, an expansion portion 2111 is provided in the first cavity 211. The expansion portion 2111 communicates with the inside of the first cavity 211 and the first channel 23, and the width of the expansion portion 2111 gradually increases from the side connected to the first channel 23 towards the middle of the first cavity 211. The setting of the expansion portion 2111 can be compatible with injection devices 4 of different sizes and models for injecting and sucking liquid into the first cavity 211.

[0041] Considering the convenience and smoothness of the flow of the cryoprotectant between the first cavity 211 and the second cavity 212, the bottom of the first channel 23 extends obliquely.

[0042] Furthermore, the bottom end height of the first channel 23 connected to the first cavity 211 is lower than the bottom end height of the first channel 23 connected to the second cavity 212. This can ensure that after the cryoprotectant pre-cryopreprocesses the biological materials in the second cavity 212, it can quickly flow back into the first cavity 211, and then the cryoprotectant can be quickly sucked out of the first cavity 211.

[0043] Please refer to Figure 3 and Figure 4 In an embodiment where the structure of the first channel 23 is formed, a first mating portion 2112 is provided at the end of the first cavity 211, and a second mating portion 2121 is provided at the end of the second cavity 212. The first mating portion 2112 and the second mating portion 2121 are staggered in position to form the first channel 23. This setting method can conveniently and effectively form the first channel 23, and solves the technical problem of difficult processing of the first channel 23 to meet smaller sizes. It can be achieved that through direct injection molding, the first channel 23 can reach a gap of 0.08 mm, while it is very difficult to achieve such a small-sized gap by other methods. Based on this structure, it can be realized that only by injecting liquid into the first cavity 211, the liquid will actively flow into the second cavity 212 to pre-cryopreprocess the biological materials. After the treatment, by sucking liquid in the first cavity 211, the liquid in the second cavity 212 will flow into the first cavity 211 through the first channel 23 and be sucked away. During this operation process, there will be no loss of biological materials or the phenomenon that biological materials flow into the first cavity 211 through the first channel 23.

[0044] Furthermore, the first engaging portion 2112 and the second engaging portion 2121 are spaced apart in a direction perpendicular to the liquid flow direction, and the end face of the first engaging portion 2112 facing the second cavity 212 is flush with the end face of the second engaging portion 2121 facing the first cavity 211 or staggered in the liquid flow direction, ensuring smoother and faster liquid flow.

[0045] In one embodiment, the second channel 24 is a circular channel, and the opening valve 22 has a cylindrical end that can be inserted into the second channel 24 and seal the second channel 24, forming a more smoothly pluggable and unplugable fitting structure.

[0046] Please refer to Figure 2 、 Figure 5 and Figure 7 , furthermore, the opening valve 22 has opposite top and bottom ends along its length direction. A first flange 221 protrudes from the outer periphery of the top end. The first material taking member 5 includes a material moving groove 51 extending inward from the end portion. The material moving groove 51 can accommodate the top end to slide in, and the top of the first material taking member 5 abuts against the first flange 221. When the first material taking member 5 moves upward, the opening valve 22 can be taken out of the second channel 24. The bottom end is a cylindrical end.

[0047] A second flange 222 protrudes from the outer periphery of the cylindrical end. The second flange 222 is annular, and the second flange 222 is in close fit with the second channel 24, enabling the opening valve 22 to quickly seal the second channel 24 with better sealing effect.

[0048] It can be understood that the bottom part of the second channel 24 exposed from the body 21 can be conical, which is conducive to the outflow of biological materials from the second channel 24 and can quickly flow out without damaging the biological materials.

[0049] In another embodiment, the automated processing device 100 for vitrification of biological materials further includes a driving device. The driving end of the driving device is connected to the moving carrier 3, and the driving device can drive the liquid injection device 4 and the first material taking member 5 to move horizontally, vertically and vertically.

[0050] Please refer to Figure 2, In a specific embodiment, the mobile carrier 3 includes a first base body 31, a second base body 32 and a third base body 33. The driving device includes a first driving member 61, a second driving member 62 and a third driving member 63. The first base body 31 is slidably arranged on the frame 1 in the transverse direction, the second base body 32 is slidably arranged on the first base body 31 in the longitudinal direction, and the third base body 33 is slidably arranged on the second base body 32 in the vertical direction. The driving end of the first driving member 61 is connected to the first base body 31 in the transverse direction, the driving end of the second driving member 62 is connected to the second base body 32 in the longitudinal direction, and the driving end of the third driving member 63 is connected to the third base body 33 in the vertical direction. The liquid injection device 4 and the first material taking member 5 are arranged on the third base body 33.

[0051] Please refer to Figure 2 , It should be noted that, in order to make the driving smoother and the positioning more accurate, the first driving member 61, the second driving member 62 and the third driving member 63 can all adopt the combined structure of a motor and a ball screw. Specifically, the first driving member 61 includes a first motor 611 and a first ball screw 612. A first guide rail 613 is arranged on the frame 1 extending in the transverse direction. The first motor 611 is arranged on the frame 1. The screw rod of the first ball screw 612 extends in the transverse direction and is rotatably arranged on the frame 1. The output end of the first motor 611 is connected to the screw rod of the first ball screw 612. The first base body 31 is connected to the nut of the first ball screw 612. By the rotation drive of the first motor 611, the screw rod of the first ball screw 612 rotates, and the nut of the first ball screw 612 drives the first base body 31 to move along the first guide rail 613.

[0052] Please refer to Figure 8 , Similarly, the setting structure of the second driving member 62 can also refer to that of the first driving member 61. The second driving member 62 includes a second motor 621 and a second ball screw 622. A second guide rail 623 is arranged on the first base body 31 extending in the longitudinal direction. The second motor 621 is arranged on the first base body 31. The screw rod of the second ball screw 622 extends in the longitudinal direction and is rotatably arranged on the first base body 31. The output end of the second motor 621 is connected to the screw rod of the second ball screw 622. The second base body 32 is connected to the nut of the second ball screw 622. By the rotation drive of the second motor 621, the screw rod of the second ball screw 622 rotates, and the nut of the second ball screw 622 drives the second base body 32 to move along the second guide rail 623.

[0053] Please refer to Figure 5 and Figure 6, the third driving member 63 includes a third motor 631 and a third ball screw 632. A third guide rail 633 is vertically extended on the second base 32. The third motor 631 is disposed on the top of the second base 32. The screw rod of the third ball screw 632 extends vertically and is rotatably disposed on the second base 32. The output end of the third motor 631 is connected to the screw rod of the third ball screw 632. The third base 33 is connected to the nut of the third ball screw 632. By the rotation of the third motor 631, the screw rod of the third ball screw 632 rotates, and the third base 33 is driven by the nut of the third ball screw 632 to move along the third guide rail 633.

[0054] Please refer to Figure 1 and Figure 6 , in another embodiment, the automated processing device 100 for vitrification of biological materials further includes a second material taking member 7, a loading rod 8 and a liquid nitrogen bearing device 9. The loading rod 8 is horizontally inserted into the frame 1 and is directly below the end of the second channel 24. The second material taking member 7 includes a fourth driving member 110, a first clamping arm 120 and a second clamping arm 130. The fourth driving member 110 is vertically slidably disposed on the second base 32. The first clamping arm 120 and the second clamping arm 130 are vertically spaced apart, and the first clamping arm 120 and the second clamping arm 130 are connected to the driving end of the fourth driving member 110. The fourth driving member 110 can drive the first clamping arm 120 and the second clamping arm 130 to move towards each other to clamp the loading rod 8, drive the first clamping arm 120 and the second clamping arm 130 to move away from each other to release the loading rod 8, and drive the first clamping arm 120 and the second clamping arm 130 to rotate to tilt the loading rod 8. The liquid nitrogen bearing device 9 is disposed on the frame 1, and its top is open and filled with liquid nitrogen. The fourth driving member 110 drives the first clamping arm 120 and the second clamping arm 130 to clamp the loading rod 8 with the cryopreserved biological material and move it to the top of the liquid nitrogen bearing device 9. Then, the fourth driving member 110 drives the first clamping arm 120 and the second clamping arm 130 to rotate to tilt the loading rod 8, so that the biological material is immersed in the liquid nitrogen bearing device 9.

[0055] Please refer to Figure 7 , in one embodiment, the liquid injection device 4 includes a liquid injection head 41 and a liquid suction head 42. The liquid injection head 41 can inject liquid into the first cavity 211, and the liquid suction head 42 can suck out the liquid in the first cavity 211.

[0056] In another embodiment, a first vision detector 140, a second vision detector, and a pressurizing device 150 are also provided. The processing member 2 is a transparent member. The first vision detector 140 is disposed on the frame 1 and is located directly in front of the processing member 2. The first vision detector 140 can detect the liquid conditions in the first cavity 211 and the second cavity 212, and detect the state of the biological material in the second cavity 212. The first vision detector 140 and the second vision detector can be a CCD vision detection system. The pressurizing device 150 is disposed on the third base 33 and is spaced from the liquid injection device 4. The pressurizing device 150 can be driven by the moving carrier 3 to pressurize the second channel 24, so that the biological material in the second cavity 212 is pressured and falls from the second channel 24 onto the carrier rod 8. The pressurizing device 150 can adopt a pneumatic method. The second vision detector is disposed on the frame 1 and can detect the situation of the biological material flowing onto the carrier rod 8.

[0057] In actual operation, at least two liquid injection operations are required, and the specific operation requirements can be adjusted according to the actual situation and are not limited here.

[0058] The specific steps are as follows:

[0059] The first liquid injection operation: First, inject ES balanced solution into the first cavity 211 through the liquid injection head 41. The ES balanced solution will flow into the second cavity 212 through the first channel 23 and act on the biological material. After the ES balanced solution has acted on the biological material, then suck out the ES balanced solution from the first cavity 211 through the liquid suction head 42;

[0060] The second liquid injection operation: Inject VS cryoprotectant into the first cavity 211 through the liquid injection head 41. The VS cryoprotectant will flow into the second cavity 212 through the first channel 23 and act on the biological material. After the VS cryoprotectant has acted on the biological material, then suck out the VS cryoprotectant from the first cavity 211 through the liquid suction head 42;

[0061] Pull out the opening valve 22: Drive the first material taking member 5 to move to the opening valve 22, and make the top of the opening valve 22 slide into the material transfer groove 51. Drive the first material taking member 5 so that the top of the first material taking member 5 abuts against the first flange 221, and the first material taking member 5 moves upward to take out the opening valve 22 from the second channel 24;

[0062] Biological material transfer: The pressurizing device 150 moves to the second channel 24 and pressurizes the second channel 24, so that the biological material falls from the second channel 24 onto the carrier rod 8;

[0063] Transfer to liquid nitrogen treatment: The fourth driving member 110 moves and drives the first clamping arm 120 and the second clamping arm 130 to clamp the end of the carrier rod 8, and withdraw it laterally. The first clamping arm 120 and the second clamping arm 130 drive the carrier rod 8 directly above the liquid nitrogen carrying device 9, and drive the first clamping arm 120 and the second clamping arm 130 to rotate through the fourth driving member 110, so that the carrier rod 8 is tilted to immerse the biological material in liquid nitrogen.

[0064] It should be noted that when the automated processing device 100 of the present invention preprocesses biological materials, multiple biological materials can be preprocessed simultaneously by increasing the number of processing members 2, liquid injection devices 4, and first material taking members 5. The specific number of settings of the processing members 2, liquid injection devices 4, and first material taking members 5 can be 3, 4, 6, 8, etc. The number can be odd or even, and there is no limit here.

[0065] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification.

[0066] The above-described embodiments only represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations, substitutions, and improvements can be made, and these should all be covered by the protection scope of the present invention. Therefore, the protection scope of the invention patent should be based on the claims.

Claims

1. An automated processing device for vitrification of biological materials, characterized in that, Comprising: Frame; Processing member, disposed on the frame, the processing member includes a main body and an opening valve, the main body is provided with a first cavity and a second cavity, the first cavity and the second cavity are communicated through a first channel, and the first channel only allows liquid to pass through. A second channel communicating with the second cavity and the outside is provided in the main body. The opening valve is detachably disposed in the second channel, and the opening valve can seal the second channel. A first mating portion is provided at an end of the first cavity, and a second mating portion is provided at an end of the second cavity. The first mating portion and the second mating portion are offset in position to form the first channel. The first mating portion and the second mating portion are spaced apart in a direction perpendicular to the liquid flow direction, and an end face of the first mating portion facing the second cavity is flush with or staggered along the liquid flow direction with an end face of the second mating portion facing the first cavity; Moving carrier, movably disposed on the frame; Liquid injection device, disposed on the moving carrier, and the liquid injection device can inject or suck liquid into the first cavity under the drive of the moving carrier; And First picking member, disposed on the moving carrier, and the first picking member can take out the opening valve from the second channel under the drive of the moving carrier.

2. The automated processing equipment for vitrification of biological materials according to claim 1, characterized in that, The second channel is a circular channel, the opening valve has a cylindrical end, and the cylindrical end can be inserted into the second channel and seal the second channel.

3. The automated processing equipment for vitrification of biological materials according to claim 2, characterized in that, The opening valve has an opposite top end and bottom end. A first flange protrudes on the outer periphery of the top end. The first picking member includes a material moving groove extending inward from the end. The material moving groove can allow the top end to slide in, and the top of the first picking member abuts against the first flange. The first picking member moves upward and can take out the opening valve; the bottom end is a cylindrical end.

4. The automated processing equipment for vitrification of biological materials according to claim 3, characterized in that, A second flange protrudes on the outer periphery of the cylindrical end, the second flange is annular, and the second flange is in close fit with the second channel.

5. The automated processing equipment for vitrification of biomaterials according to claim 1, characterized in that, It further includes a driving device, a driving end of the driving device is connected to the moving carrier, and the driving device can drive the liquid injection device and the first picking member to move horizontally, longitudinally and vertically.

6. The automated processing equipment for vitrification of biological materials according to claim 5, characterized in that, The moving carrier includes a first base body, a second base body and a third base body. The driving device includes a first driving member, a second driving member and a third driving member. The first base body is slidably disposed on the frame horizontally, the second base body is slidably disposed on the first base body longitudinally, and the third base body is slidably disposed on the second base body vertically. A driving end of the first driving member is connected to the first base body horizontally, a driving end of the second driving member is connected to the second base body longitudinally, and a driving end of the third driving member is connected to the third base body vertically; the liquid injection device and the first picking member are disposed on the third base body.

7. The automated processing equipment for vitrification of biological materials according to claim 6, characterized in that, It further includes a second material taking member and a loading rod. The loading rod is inserted into the frame and is located directly below the second channel. The second material taking member includes a fourth driving member, a first clamping arm and a second clamping arm. The fourth driving member is slidably arranged on the second base body in the vertical direction. The first clamping arm and the second clamping arm are spaced apart in the vertical direction, and the first clamping arm and the second clamping arm are connected to the driving end of the fourth driving member. The fourth driving member can drive the first clamping arm and the second clamping arm to move towards each other to clamp the loading rod, drive the first clamping arm and the second clamping arm to move away from each other to release the loading rod, and drive the first clamping arm and the second clamping arm to rotate to tilt the loading rod.

8. The automated processing device for vitrification of biological materials according to claim 1, characterized in that, The liquid injection device includes a liquid injection head and a liquid suction head. The liquid injection head can inject liquid into the first cavity, and the liquid suction head can suck out the liquid in the first cavity.

9. The automated processing equipment for vitrification of biomaterials according to claim 1, wherein It further includes a first vision detector. The processing member is a transparent member. The first vision detector is arranged on the frame and is located directly in front of the processing member. The first vision detector can detect the liquid conditions in the first cavity and the second cavity, and detect the state of the biological material in the second cavity.

10. The automated processing equipment for vitrification of biological materials according to claim 1, characterized in that, It further includes a loading rod and a second vision detector. The loading rod is arranged directly below the second channel. The second vision detector is arranged on the frame and can detect the situation of the biological material falling onto the loading rod.

11. The automated processing device for vitrification of biomaterials according to claim 10, characterized in that, It further includes a pressurizing device. The pressurizing device is arranged on the moving carrier and can pressurize the second channel along with the driving of the moving carrier, so that the biological material in the second cavity falls onto the loading rod through the second channel.

Images