Magnetic rod assembly, nucleic acid extraction device and liquid processing method

Abstract

The present invention provides a magnetic rod assembly, a nucleic acid extraction device and a liquid processing method, comprising a magnetic rod, an inner magnetic rod sleeve and an outer magnetic rod sleeve; the inner magnetic rod sleeve and the outer magnetic rod sleeve respectively comprise a hollow rod sleeve body having two ends; wherein: the first end of the two ends of the inner magnetic rod sleeve is closed, and the second end is open; the cross-sectional dimension of at least part of the outer wall of the magnetic rod is less than or equal to the cross-sectional dimension of the inner wall of the inner magnetic rod sleeve, and the magnetic rod can be selectively inserted into the inner magnetic rod sleeve from the second end of the inner magnetic rod sleeve or separated from the inner magnetic rod sleeve; the first end and the second end of the two ends of the outer magnetic rod sleeve are both open; the cross-sectional dimension of the inner wall of the outer magnetic rod sleeve is closely matched with the cross-sectional dimension of at least part of the outer wall of the inner magnetic rod sleeve, so that the inner magnetic rod sleeve can be inserted into the outer magnetic rod sleeve from the second end of the outer magnetic rod sleeve and reciprocate along the inner wall of the outer magnetic rod sleeve.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro diagnosis, and in particular to a magnetic rod assembly, a nucleic acid extraction device and a liquid processing method. Background Art

[0002] The use of magnetic beads for nucleic acid extraction is an effective technical means for gene detection and gene extraction. It has been widely used in many fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, animal husbandry and molecular biology research. With the continuous development of nucleic acid extraction technology, high-throughput and automated nucleic acid extraction technology has become the development trend of the industry.

[0003] In the prior art, there are two main modes for nucleic acid extraction automation: one is to use an automated nucleic acid extractor, and the other is to use an automated workstation.

[0004] However, automated nucleic acid extraction instruments can only realize automated transfer of magnetic beads, but cannot perform operations such as adding, aspirating, and transferring sample liquid in the sample plate; automated workstations can perform operations such as adding, aspirating, and transferring sample liquid, but cannot perform operations such as magnetic separation on magnetic beads.

[0005] It can be seen that the prior art cannot use the same device to achieve integrated operations on magnetic beads and sample liquid. Summary of the invention

[0006] The invention provides a magnetic rod assembly, a nucleic acid extraction device and a liquid processing method to solve the problem that the operation of magnetic beads and sample liquid cannot be achieved in an integrated manner by using the same device.

[0007] According to a first aspect of the present invention, there is provided a magnetic bar assembly, comprising a magnetic bar, an inner magnetic bar sleeve and an outer magnetic bar sleeve;

[0008] The inner layer magnetic rod sleeve and the outer layer magnetic rod sleeve each include a hollow rod sleeve body with two ends; wherein:

[0009] The first end of the two ends of the inner magnetic rod sleeve is closed, and the second end is open; the cross-sectional size of at least part of the outer wall of the magnetic rod is smaller than or equal to the cross-sectional size of the inner wall of the inner magnetic rod sleeve, and the magnetic rod can be selectively inserted into the inner magnetic rod sleeve from the second end of the inner magnetic rod sleeve or separated from the inner magnetic rod sleeve;

[0010] The first end and the second end of the two ends of the outer magnetic rod sleeve are both open; the cross-sectional size of the inner wall of the outer magnetic rod sleeve is closely adapted to the cross-sectional size of at least part of the outer wall of the inner magnetic rod sleeve, so that the inner magnetic rod sleeve can be inserted into the outer magnetic rod sleeve from the second end of the outer magnetic rod sleeve and reciprocate along the inner wall of the outer magnetic rod sleeve.

[0011] Optionally, the assembly state of the magnetic bar assembly includes a first assembly state and a second assembly state;

[0012] When in the first assembly state, the magnetic bar is inserted into the inner magnetic bar sleeve, and the inner magnetic bar sleeve is inserted into the outer magnetic bar sleeve;

[0013] In the second assembly state, the inner magnetic rod sleeve is inserted into the outer magnetic rod sleeve, and the magnetic rod is not inserted into the inner magnetic rod sleeve.

[0014] Optionally, in the first assembly state, the magnetic bar assembly is used to:

[0015] When the magnetic rod is inserted into the first end portion contacting the inner magnetic rod sleeve, the first end portion of the inner magnetic rod sleeve is inserted into the outer magnetic rod sleeve, and the first end portion of the outer magnetic rod sleeve is inserted into the sample solution containing magnetic beads, the magnetic beads in the sample solution are adsorbed onto the outer side of the inner magnetic rod sleeve and / or the outer side of the outer magnetic rod sleeve.

[0016] Optionally, the magnetic bar assembly is used for:

[0017] When the magnetic bar assembly in the first assembly state absorbs magnetic beads and the magnetic bar is drawn out of the inner magnetic bar sleeve or loses its magnetic force, the magnetic beads fall off the magnetic bar assembly.

[0018] Optionally, in the first assembly state, the magnetic bar assembly is used to:

[0019] When a sample liquid containing magnetic beads is inserted into the first end of the outer magnetic rod sleeve and the inner magnetic rod sleeve moves along the inner wall of the outer magnetic rod sleeve away from the first end of the outer magnetic rod sleeve, the sample liquid is extracted into the outer magnetic rod sleeve, and the magnetic beads in the sample liquid are adsorbed on the outer side of the inner magnetic rod sleeve.

[0020] Optionally, in the first assembly state, the magnetic bar assembly is used to:

[0021] Magnetic beads are adsorbed on the outside of the inner magnetic rod sleeve, and sample liquid is contained in the outer magnetic rod sleeve. When the inner magnetic rod sleeve carries the magnetic rod and moves along the inner wall of the outer magnetic rod sleeve close to the first end of the outer magnetic rod sleeve, the sample liquid without the magnetic beads is released to the outside of the outer magnetic rod sleeve.

[0022] Optionally, when in the second assembly state, the magnetic rod assembly is used to: insert the sample liquid into the first end of the outer magnetic rod sleeve, and when the inner magnetic rod sleeve moves along the inner wall of the outer magnetic rod sleeve away from the first end of the outer magnetic rod sleeve, the sample liquid is extracted into the outer magnetic rod sleeve.

[0023] Optionally, in the second assembly state, the magnetic bar assembly is used to:

[0024] The outer magnetic rod sleeve contains sample liquid, and when the inner magnetic rod sleeve moves along the inner wall of the outer magnetic rod sleeve and approaches the first end of the outer magnetic rod sleeve, the sample liquid is released to the outside of the outer magnetic rod sleeve.

[0025] Optionally, in the second assembly state, the magnetic bar assembly is used to:

[0026] The sample liquid is inserted into the first end of the outer magnetic rod sleeve, and when the outer magnetic rod sleeve is operated to perform stirring motion in the sample liquid, the sample liquid is mixed.

[0027] Optionally, the cross-sectional shape of the magnetic bar is at least one of the following:

[0028] Round;

[0029] Polygon;

[0030] Oval.

[0031] Optionally, the cross-sectional shape of the magnetic rod matches the cross-sectional shape of the inner wall of the inner magnetic rod sleeve.

[0032] Optionally, the cross-sectional shape of the inner magnetic rod sleeve and the cross-sectional shape of the outer magnetic rod sleeve are both circular; the cross-sectional shape of the outer wall of the inner magnetic rod sleeve and the cross-sectional shape of the inner wall of the outer magnetic rod sleeve are both circular.

[0033] Optionally, the length of the magnetic bar is greater than the length of the inner magnetic bar sleeve; the length of the inner magnetic bar sleeve is greater than the length of the outer magnetic bar sleeve.

[0034] Optionally, a pull rod is provided at one end of the magnetic rod.

[0035] Optionally, the material of the magnetic bar includes a permanent magnet or an electromagnet.

[0036] Optionally, when the material of the magnetic bar is an electromagnet, the magnetic bar assembly further includes an electromagnetic control device, and the electromagnetic control device is electrically connected to the electromagnet.

[0037] According to a second aspect of the present invention, there is provided a device for extracting nucleic acid, comprising the magnetic bar assembly of the first aspect of the present invention and its optional solutions.

[0038] According to a third aspect of the present invention, there is provided a method for treating liquid using the nucleic acid extraction device according to the second aspect of the present invention and its optional solutions, comprising: treating magnetic beads and / or treating liquid; the assembly state of the magnetic bar assembly comprises a first assembly state and a second assembly state; the first end of the magnetic bar assembly is the first end of the outer magnetic bar sleeve;

[0039] in:

[0040] The processing of the magnetic beads includes at least one of the following:

[0041] Separation of magnetic beads: inserting the first end of the magnetic rod assembly in the first assembly state into a first sample solution containing magnetic beads to adsorb the first magnetic beads in the first sample solution;

[0042] Transfer of magnetic beads: when the magnetic bar assembly in the first assembly state absorbs the second magnetic beads, the magnetic bar is withdrawn from the inner magnetic bar sleeve or the magnetic bar is controlled to lose its magnetic force, so that: the second magnetic beads fall off from the magnetic bar assembly;

[0043] Mixing the magnetic beads: inserting the first end of the magnetic bar assembly in the second assembly state into a third sample solution containing third magnetic beads, and driving the magnetic bar assembly to perform stirring motion in the third sample solution, so that: the third magnetic beads in the third sample solution are mixed;

[0044] The treatment of the liquid includes at least one of the following:

[0045] Liquid aspiration: inserting the first end of the magnetic rod assembly in the second assembly state into the fourth sample liquid, driving the inner magnetic rod sleeve to move along the inner wall of the outer magnetic rod sleeve away from the first end of the outer magnetic rod sleeve, so that: the fourth sample liquid is extracted into the outer magnetic rod sleeve;

[0046] Adding liquid: when the outer magnetic rod sleeve of the magnetic rod assembly in the second assembly state contains the fifth sample liquid, driving the inner magnetic rod sleeve to move along the inner wall of the outer magnetic rod sleeve close to the first end of the outer magnetic rod sleeve, so that: the fifth sample liquid is released from the outer magnetic rod sleeve;

[0047] Separating the magnetic beads and sucking out the liquid at the same time: inserting the first end of the magnetic bar assembly in the first assembly state into the sixth sample liquid containing the fourth magnetic beads, driving the inner magnetic bar sleeve to move along the inner wall of the outer magnetic bar sleeve away from the first end of the outer magnetic bar sleeve, so that: the sixth sample liquid is extracted into the outer magnetic bar sleeve, and the fourth magnetic beads are adsorbed outside the inner magnetic bar sleeve;

[0048] Adding liquid while separating magnetic beads: when the magnetic bar assembly in the first assembly state has fifth magnetic beads adsorbed, and the outer magnetic bar sleeve has seventh sample liquid, driving the inner magnetic bar sleeve to carry the magnetic bar along the inner wall of the outer magnetic bar sleeve and move close to the first end of the outer magnetic bar sleeve, so that: the magnetic bar assembly adsorbs the fifth magnetic beads and releases the seventh sample liquid without magnetic beads to the outside of the outer magnetic bar sleeve;

[0049] Mixing of liquid: inserting the first end of the magnetic rod assembly in the second assembly state into the eighth sample liquid, and driving the magnetic rod assembly to perform stirring motion in the eighth sample liquid, so that: the eighth sample liquid is mixed.

[0050] The magnetic bar assembly, nucleic acid extraction device and liquid processing method provided by the present invention can realize the individual operation of magnetic beads (such as separation, transfer and mixing of magnetic beads), the individual operation of sample liquid (such as addition, suction, mixing and transfer of liquid) and the simultaneous operation of magnetic beads and sample liquid (such as suction of liquid while separating magnetic beads and addition of liquid while separating magnetic beads) through the mutual cooperation and relative movement between the magnetic bar, the inner magnetic bar cover and the outer magnetic bar cover, thereby enriching the functions of the magnetic bar assembly based on the magnetic bar and the magnetic bar cover, simplifying the equipment structure of nucleic acid extraction, and helping to improve the operational efficiency of nucleic acid extraction. At the same time, it provides a basis for the full automation of various operations in nucleic acid extraction technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0052] Figure 1 The structure of the magnetic rod assembly in one embodiment of the present invention is shown in FIG. Figure 1 ;

[0053] Figure 2 The structure of the magnetic rod assembly in one embodiment of the present invention is shown in FIG. Figure 2 ;

[0054] Figure 3 The structure of the magnetic rod assembly in one embodiment of the present invention is shown in FIG. Figure 3 ;

[0055] Figure 4a is a structural schematic diagram of a magnetic bar assembly in a first assembly state according to an embodiment of the present invention;

[0056] Figure 4bis a structural schematic diagram of a magnetic bar assembly in a second assembly state according to an embodiment of the present invention;

[0057] Figure 5a It is a schematic structural diagram of a magnetic bar assembly used for separation of magnetic beads in one embodiment of the present invention;

[0058] Figure 5b The structure of the magnetic rod assembly used for adding magnetic beads in one embodiment of the present invention is shown in FIG. Figure 1 ;

[0059] Figure 5c The structure of the magnetic rod assembly used for adding magnetic beads in one embodiment of the present invention is shown in FIG. Figure 2 ;

[0060] Figure 6 It is a schematic diagram of the structure of a magnetic rod assembly used for separating magnetic beads and sucking out liquid at the same time in one embodiment of the present invention;

[0061] Figure 7 It is a schematic diagram of the structure of a magnetic rod assembly when a liquid is added while separating magnetic beads in one embodiment of the present invention;

[0062] Figure 8 It is a schematic diagram of a partially enlarged structure of a magnetic rod assembly when adding liquid while separating magnetic beads in one embodiment of the present invention;

[0063] Fig. 9 It is a schematic structural diagram of a magnetic rod assembly used for aspirating a sample liquid in one embodiment of the present invention;

[0064] Fig.10 It is a schematic structural diagram of a magnetic rod assembly used for adding a sample solution in one embodiment of the present invention;

[0065] Fig.11 Schematic diagram 4 of the structure of the magnetic rod assembly in one embodiment of the present invention;

[0066] Fig.12 Schematic diagram 5 of the structure of the magnetic rod assembly in one embodiment of the present invention;

[0067] Description of reference numerals:

[0068] 1-Magnetic bar;

[0069] 11-magnetic bar segment;

[0070] 12-magnetic bar segment;

[0071] 13-tie rod;

[0072] 2-Inner magnetic rod sleeve;

[0073] 21- a first end portion;

[0074] 22- second end;

[0075] 23-inner magnetic rod sleeve section;

[0076] 24-inner magnetic rod sleeve section;

[0077] 25-inner magnetic rod sleeve section;

[0078] 3-Outer magnetic rod sleeve;

[0079] 31- a first end portion;

[0080] 32- second end portion;

[0081] 4- sample solution;

[0082] 41- magnetic beads;

[0083] 5-sample liquid;

[0084] 6-Electromagnetic control device. DETAILED DESCRIPTION

[0085] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0086] The terms "first", "second", "third", "fourth", etc. (if any) in the specification and claims of the present invention and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the present invention described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units that are clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0087] The technical solution of the present invention is described in detail with specific embodiments below. The following specific embodiments can be combined with each other, and the same or similar concepts or processes may not be described in detail in some embodiments.

[0088] Please refer to Figure 1 The magnetic bar assembly includes a magnetic bar 1, an inner magnetic bar sleeve 2 and an outer magnetic bar sleeve 3;

[0089] The inner layer magnetic rod sleeve 2 and the outer layer magnetic rod sleeve 3 each include a hollow rod sleeve body with two ends; wherein:

[0090] The magnetic bar 1 can be understood as a bar having magnetic force or being able to generate magnetic force.

[0091] The first end 21 of the two ends of the inner magnetic rod sleeve 2 is closed, and the second end 22 is open; the cross-sectional size of at least part of the outer wall of the magnetic rod 1 is less than or equal to the cross-sectional size of the inner wall of the inner magnetic rod sleeve 2, wherein the cross-sectional size of at least part of the outer wall of the magnetic rod 1 is less than or equal to the cross-sectional size of the inner wall of the inner magnetic rod sleeve 2, which can be understood as: at least part of the magnetic rod segment can be inserted into the inner magnetic rod sleeve 2, and the cross-sectional size of the magnetic rod segment inserted into the inner magnetic rod sleeve 2 is less than or equal to the cross-sectional size of the inner wall of the inner magnetic rod sleeve 2, so that the magnetic rod 1 can be inserted or removed from the second end of the inner magnetic rod sleeve 2. The magnetic rod 1 can be selectively inserted into the inner magnetic rod sleeve 2 or separated from the inner magnetic rod sleeve 2 from the second end 22 of the inner magnetic rod sleeve.

[0092] For an example, please refer to Figure 2 The cross-sectional dimension of the magnetic rod segment 11 inserted into the inner magnetic rod sleeve 2 is smaller than the cross-sectional dimension of the inner wall of the inner magnetic rod sleeve 2, and the cross-sectional dimension of the magnetic rod segment 12 not inserted into the inner magnetic rod sleeve 2 is larger than the cross-sectional dimension of the inner wall of the inner magnetic rod sleeve 2; in other examples, the cross-sectional dimension of the magnetic rod segment 11 may also be equal to (or close to) the cross-sectional dimension of the inner wall of the inner magnetic rod sleeve 2, and the cross-sectional dimension of the magnetic rod segment 12 may also be equal to (or close to) the cross-sectional dimension of the magnetic rod segment 11.

[0093] The magnetic bar 1 may be a structure that can generate a magnetic field as a whole or partially. For example, only the magnetic bar segment 11 may generate a magnetic field, or both the magnetic bar segment 11 and the magnetic bar segment 12 may generate a magnetic field.

[0094] Please refer to Figure 1 and Figure 3 The first end 31 and the second end 32 of the two ends of the outer magnetic rod sleeve 3 are both open; the cross-sectional size of the inner wall of the outer magnetic rod sleeve 3 is closely adapted to the cross-sectional size of at least part of the outer wall of the inner magnetic rod sleeve 2, so that the inner magnetic rod sleeve 2 can be inserted into the outer magnetic rod sleeve 3 from the second end 32 of the outer magnetic rod sleeve 3 and reciprocate along the inner wall of the outer magnetic rod sleeve 3.

[0095] Among them, the inner wall cross-sectional size of the outer magnetic rod sleeve 3 is closely matched with the cross-sectional size of at least part of the outer wall of the inner magnetic rod sleeve 2, which can be understood as: at least part of the inner magnetic rod sleeve segment can be inserted into the outer magnetic rod sleeve 3, and in the inner magnetic rod sleeve segment inserted into the outer magnetic rod sleeve 3, the outer wall cross-sectional size of part of the inner magnetic rod sleeve segment is equal to (or close to) the inner wall cross-sectional size of the outer magnetic rod sleeve 3.

[0096] For an example, please refer to Figure 3 The outer wall cross-sectional dimension of the inner magnetic rod sleeve segment 23 inserted into the outer magnetic rod sleeve 3 is equal to (or close to) the inner wall cross-sectional dimension of the outer magnetic rod sleeve 3, the outer cross-sectional dimension of the inner magnetic rod sleeve segment 24 inserted into the outer magnetic rod sleeve 3 is smaller than the inner wall cross-sectional dimension of the outer magnetic rod sleeve 3, and the outer wall cross-sectional dimension of the inner magnetic rod sleeve segment 25 not inserted into the outer magnetic rod sleeve is larger than the cross-sectional dimension of the inner wall of the outer magnetic rod sleeve 3; in other examples, the outer wall cross-sectional dimensions of the inner magnetic rod sleeve segment 23, the inner magnetic rod sleeve segment 24 and the inner magnetic rod sleeve segment 25 can all be equal to (or close to) the inner wall cross-sectional dimension of the outer magnetic rod sleeve 3.

[0097] For further information, please refer to Figure 4a and Figure 4b , the assembly state of the magnetic bar assembly includes a first assembly state and a second assembly state;

[0098] In the first assembly state, please refer to Figure 4a , the magnetic bar 1 is inserted into the inner magnetic bar sleeve 2, and the inner magnetic bar sleeve 2 is inserted into the outer magnetic bar sleeve 3;

[0099] In the second assembly state, please refer to Figure 4b , the inner magnetic rod sleeve 2 is inserted into the outer magnetic rod sleeve 3 , and the magnetic rod 1 is not inserted into the inner magnetic rod sleeve 2 .

[0100] For further information, please refer to Figure 5a , when in the first assembly state, the magnetic bar assembly is used to:

[0101] When the magnetic bar 1 is inserted into the first end 21 of the inner magnetic rod sleeve, the first end 21 of the inner magnetic rod sleeve is inserted into the outer magnetic rod sleeve 3, and the first end 31 of the outer magnetic rod sleeve 3 is inserted into the sample liquid 4 containing magnetic beads, the magnetic beads 41 in the sample liquid are adsorbed on the outside of the inner magnetic rod sleeve 2 and / or the outer magnetic rod sleeve 3, wherein the outside of the inner magnetic rod sleeve 2 can be understood as: the outer wall of the inner magnetic rod sleeve 2, or the inner wall of the outer magnetic rod sleeve 3, that is, the magnetic beads 41 can be adsorbed on the outer wall of the inner magnetic rod sleeve 2, or on the inner wall of the outer magnetic rod sleeve 3, and can also be adsorbed on the outer wall of the outer magnetic rod sleeve 3.

[0102] The magnetic bar assembly described above can be understood as being used for separating magnetic beads, for example, separating the magnetic beads 41 from the sample liquid 4 containing the magnetic beads 41 .

[0103] For further information, please refer to Figure 5b , the magnetic bar assembly is used for:

[0104] When the magnetic bar assembly in the first assembly state absorbs the magnetic beads 41 and the magnetic bar 1 is pulled out of the inner magnetic bar sleeve 2 or loses its magnetic force, the magnetic beads 41 fall off from the magnetic bar assembly.

[0105] Among them, please refer to Figure 5c The magnetic bar 1 loses its magnetic force, for example, when the magnetic bar 1 is an electromagnet and the electromagnet is powered off, the magnetic bar loses its magnetic force.

[0106] The magnetic bar assembly described above can be understood as the addition of magnetic beads, for example, adding the magnetic beads 41 into the sample liquid 5 .

[0107] If the operation of separating the magnetic beads 41 is combined with the operation of adding the magnetic beads 41 , for example, first separating the magnetic beads and then adding the magnetic beads, the magnetic rod assembly can be understood as being used to transfer the magnetic beads 41 , for example, transferring the magnetic beads 41 in the sample liquid 4 to the sample liquid 5 .

[0108] For further information, please refer to Figure 6 , when in the first assembly state, the magnetic bar assembly is used to:

[0109] When the sample liquid 4 containing magnetic beads 41 is inserted into the first end 31 of the outer magnetic rod sleeve, and the inner magnetic rod sleeve 2 moves along the inner wall of the outer magnetic rod sleeve 3 away from the first end 31 of the outer magnetic rod sleeve, the sample liquid 4 is extracted into the outer magnetic rod sleeve 3, and the magnetic beads 41 in the sample liquid 4 are adsorbed on the outside of the inner magnetic rod sleeve 2. At this time, the magnetic beads 41 in the sample liquid 4 are adsorbed on the outside of the inner magnetic rod sleeve 2, and the liquid in the sample liquid 4 is in the space formed by the inner wall of the outer magnetic rod sleeve 3 and the first end 21 of the inner magnetic rod sleeve 2. The outside of the inner magnetic rod sleeve 2 can be understood with reference to the position described above.

[0110] The inner magnetic rod sleeve 2 moves along the inner wall of the outer magnetic rod sleeve 3 away from the first end 31 of the outer magnetic rod sleeve, which can be understood as: the inner magnetic rod sleeve 2 and the magnetic rod 1 are pulled outward from the first end 31 of the outer magnetic rod sleeve 3 together.

[0111] The magnetic bar assembly described above can be understood as being used to separate magnetic beads and simultaneously aspirate liquid, for example, to separate magnetic beads 41 and simultaneously aspirate liquid in the sample liquid 4 .

[0112] For the corresponding reference, please refer to Figure 7 and Figure 8 , when in the first assembly state, the magnetic bar assembly is further used for:

[0113] When magnetic beads 41 are adsorbed on the outside of the inner magnetic rod sleeve 2, and sample liquid 4 is contained in the outer magnetic rod sleeve 3, and the inner magnetic rod sleeve 2 carries the magnetic bar 1 and moves along the inner wall of the outer magnetic rod sleeve 3 close to the first end 31 of the outer magnetic rod sleeve, the sample liquid without the magnetic beads 41 is released to the outside of the outer magnetic rod sleeve 3. At this time, the magnetic beads 41 in the sample liquid 4 are adsorbed on the outside of the inner magnetic rod sleeve 2, and the liquid in the sample liquid 4 is released to the outside of the magnetic rod assembly. The outside of the inner magnetic rod sleeve 2 can be understood with reference to the position described above.

[0114] The inner magnetic rod sleeve 2 moves along the inner wall of the outer magnetic rod sleeve 3 close to the first end 31 of the outer magnetic rod sleeve, which can be understood as: the inner magnetic rod sleeve 2 and the magnetic rod 1 move inwardly from the first end 31 of the outer magnetic rod sleeve 3.

[0115] The magnetic bar assembly described above can be understood as being used to separate magnetic beads while adding liquid, for example, separating magnetic beads 41 while adding liquid in the sample solution 4 .

[0116] If the operation of sucking out the liquid while separating the magnetic beads 41 is combined with the operation of adding the liquid while separating the magnetic beads 41, for example, first the magnetic beads 41 are adsorbed while driving the inner magnetic rod cover 2 and the magnetic rod 1 to be pulled out from the first end 31 of the outer magnetic rod cover 3, and then the magnetic beads 41 are adsorbed while driving the inner magnetic rod cover 2 and the magnetic rod 1 to be inserted inward from the first end 31 of the outer magnetic rod cover 3, then the magnetic rod assembly can be understood as being used to operate only on the liquid (adding, separating, transferring) for the sample liquid containing magnetic beads.

[0117] Please refer to Fig. 9 When in the second assembly state, the magnetic bar assembly is used to: insert the sample liquid 6 into the first end 31 of the outer magnetic rod sleeve, and when the inner magnetic rod sleeve 2 moves along the inner wall of the outer magnetic rod sleeve 3 away from the first end 31 of the outer magnetic rod sleeve, the sample liquid 5 is extracted into the outer magnetic rod sleeve. At this time, the extracted sample liquid 5 is in the space formed by the inner wall of the outer magnetic rod sleeve 3 and the first end 21 of the inner magnetic rod sleeve 2.

[0118] Among them, the sample liquid 5 can be a sample liquid without magnetic beads, or a sample liquid containing magnetic beads, and the movement of the inner magnetic rod sleeve 2 along the inner wall of the outer magnetic rod sleeve 3 away from the first end 31 of the outer magnetic rod sleeve can be understood as: the inner magnetic rod sleeve 2 is pulled out of the outer magnetic rod sleeve 3.

[0119] The magnetic rod assembly described above can be understood as being used for aspirating sample liquid. If the sample liquid does not contain magnetic beads, it can be understood as being used for aspirating liquid. If the sample liquid contains magnetic beads, it can also be understood as being used for aspirating sample liquid and magnetic beads simultaneously.

[0120] Please refer to Fig.10 , when in the second assembly state, the magnetic bar assembly is used to:

[0121] The outer magnetic rod sleeve 3 contains a sample liquid 5 , and when the inner magnetic rod sleeve 2 moves along the inner wall of the outer magnetic rod sleeve 3 close to the first end 31 of the outer magnetic rod sleeve, the sample liquid 5 is released to the outside of the outer magnetic rod sleeve 3 .

[0122] Among them, the sample liquid 5 can be a sample liquid without magnetic beads, or a sample liquid containing magnetic beads, and the movement of the inner magnetic rod sleeve 2 along the inner wall of the outer magnetic rod sleeve 3 close to the first end 31 of the outer magnetic rod sleeve can be understood as: the inner magnetic rod sleeve 2 is inserted into the outer magnetic rod sleeve 3.

[0123] The magnetic rod assembly described above can be understood as being used for adding sample liquid. If the sample liquid does not contain magnetic beads, it can be understood as being used for adding liquid. If the sample liquid contains magnetic beads, it can also be understood as being used for adding sample liquid and magnetic beads simultaneously.

[0124] If the above-mentioned sample liquid aspiration operation is combined with the sample liquid addition operation, for example, the sample liquid 5 is aspirated first and then the sample liquid 5 is added, the magnetic rod assembly can be understood as being used for transferring the sample liquid, wherein the sample liquid may contain magnetic beads or may not contain magnetic beads.

[0125] Furthermore, in the second assembly state, the magnetic bar assembly is used to:

[0126] The sample liquid is inserted into the first end of the outer magnetic rod sleeve 3 and is operated to perform stirring motion in the sample liquid to mix the sample liquid.

[0127] The stirring motion can be understood as the inner magnetic rod sleeve 2 and the outer magnetic rod sleeve 3 being driven to simultaneously perform at least one of reciprocating motion, mixing motion, oscillating motion, and rotating motion in the sample liquid, so that the sample liquid is fully mixed. The sample liquid may contain magnetic beads or may not contain magnetic beads.

[0128] Furthermore, the cross-sectional shape of the magnetic bar 1 can be any one of a circle, a polygon, or an ellipse, or other shapes, as long as the magnetic bar can be inserted into the inner magnetic bar sleeve 2 and can follow the inner magnetic bar sleeve 2 to reciprocate in the outer magnetic bar sleeve 3, it does not deviate from the scope of this embodiment.

[0129] Furthermore, for the convenience of processing, the cross-sectional shape of the magnetic bar 1 can match the cross-sectional shape of the inner wall of the inner magnetic bar sleeve 2, which can also be understood as: the cross-sectional shape of the magnetic bar 1 is the same as the cross-sectional shape of the inner wall of the inner magnetic bar sleeve 2. For example, the cross-sectional shape of the inner magnetic bar sleeve 2 and the cross-sectional shape of the outer magnetic bar sleeve 3 can both be circular, and the cross-sectional shape of the outer wall of the inner magnetic bar sleeve 2 and the cross-sectional shape of the inner wall of the outer magnetic bar sleeve 3 can both be circular.

[0130] Furthermore, in order to facilitate the relative movement between the magnetic bar assemblies, the length of the magnetic bar 1 is greater than the length of the inner magnetic bar sleeve 2 , and the length of the inner magnetic bar sleeve 2 is greater than the length of the outer magnetic bar sleeve 3 .

[0131] For further information, please refer to Fig.11 In order to facilitate manual operation of the magnetic rod 1, such as pulling out or inserting the magnetic rod 1, a pull rod 13 may be provided at one end of the magnetic rod, wherein the shape and material of the pull rod 13 are not limited, as long as the shape and material can facilitate extraction and insertion, it does not deviate from the scope of this embodiment.

[0132] Furthermore, the material of the magnetic bar 1 can be a permanent magnet or an electromagnet. When the magnetic bar 1 is an electromagnet, please refer to Fig.12 The magnetic bar assembly also includes an electromagnetic control device 6, which is electrically connected to the electromagnet. When the electromagnet is energized, the electromagnet generates magnetic force. It can be seen that if the magnetic bar 1 is made of an electromagnet, the process of inserting the magnetic bar 1 into the inner magnetic bar sleeve 2 in the above text is specifically: the process of inserting the electromagnet into the magnetic bar sleeve 2 and energizing the electromagnet. When the electromagnet is powered off, the electromagnet loses its magnetic force, and its function is equivalent to the process of pulling the magnetic bar 1 out of the inner magnetic bar sleeve 2 as described in the above text.

[0133] In a specific solution, at least one of the above movements of the magnetic bar 1, the inner magnetic bar sleeve 2, and the outer magnetic bar sleeve 3 can be realized by automatic control, for example, the magnetic bar 1, the inner magnetic bar sleeve 2, and the outer magnetic bar sleeve 3 can be connected to corresponding driving parts respectively, and then the driving part can drive the magnetic bar 1, the inner magnetic bar sleeve 2, and the outer magnetic bar sleeve 3. Whether automatic control is adopted or not, and how the driving is realized, it does not deviate from the scope of the embodiments of the present invention.

[0134] It can be seen that in the magnetic bar assembly provided by the present invention, the mutual cooperation and relative movement between the magnetic bar 1, the inner magnetic bar cover 2, and the outer magnetic bar cover 3 can realize the individual operation of magnetic beads (such as separation, transfer and mixing of magnetic beads), the individual operation of sample liquid (such as addition, aspiration, mixing and transfer of liquid) and the simultaneous operation of magnetic beads and sample liquid (such as separation of magnetic beads while aspirating liquid, separation of magnetic beads while adding liquid), which enriches the function of the magnetic bar assembly based on the magnetic bar and the magnetic bar cover, simplifies the equipment structure of nucleic acid extraction, helps to improve the operational efficiency of nucleic acid extraction, and at the same time, provides a basis for the full automation of various operations in nucleic acid extraction technology.

[0135] The present invention also provides a device for extracting nucleic acid, comprising the magnetic rod assembly involved in the above optional scheme.

[0136] The present invention also provides a method for treating liquid using the nucleic acid extraction device, including treating magnetic beads and / or treating liquid; the assembly state of the magnetic bar assembly includes a first assembly state and a second assembly state; the first end of the magnetic bar assembly is the first end of the outer magnetic bar sleeve;

[0137] in:

[0138] The processing of the magnetic beads includes at least one of the following:

[0139] Separation of magnetic beads: inserting the first end of the magnetic rod assembly in the first assembly state into a first sample solution containing magnetic beads to adsorb the first magnetic beads in the first sample solution;

[0140] Transfer of magnetic beads: when the magnetic bar assembly in the first assembly state absorbs the second magnetic beads, the magnetic bar is withdrawn from the inner magnetic bar sleeve or the magnetic bar is controlled to lose its magnetic force, so that: the second magnetic beads fall off from the magnetic bar assembly;

[0141] Mixing the magnetic beads: inserting the first end of the magnetic bar assembly in the second assembly state into a third sample solution containing third magnetic beads, and driving the magnetic bar assembly to perform stirring motion in the third sample solution, so that: the third magnetic beads in the third sample solution are mixed;

[0142] Among them, the first sample liquid, the second sample liquid and the third sample liquid can be the same sample liquid or different sample liquids. Correspondingly, the first magnetic beads, the second magnetic beads and the third magnetic beads can be understood as magnetic beads in the same sample liquid or magnetic beads in different sample liquids.

[0143] Furthermore, the separation of magnetic beads, the transfer of magnetic beads, and the mixing of magnetic beads can be three separate operation methods, or can be any combination of them to form an integrated operation method.

[0144] In an exemplary operation process, the magnetic beads may be separated first, then transferred, and then mixed; alternatively, the magnetic beads may be separated first, then transferred, and mixed while transferring, thereby completing the process of transferring the magnetic beads from one sample solution to another sample solution and mixing, wherein mixing the magnetic beads while transferring the magnetic beads enables the magnetic beads to fall off from the magnetic rod assembly better; in other examples, the magnetic beads may be separated, transferred, or mixed separately.

[0145] It can be seen that when the separation of magnetic beads, the transfer of magnetic beads, and the mixing of magnetic beads are implemented in combination with each other, the order of implementation is not limited, and they can be implemented one after the other or simultaneously.

[0146] The treatment of the liquid includes at least one of the following:

[0147] Liquid aspiration: inserting the first end of the magnetic rod assembly in the second assembly state into the fourth sample liquid, driving the inner magnetic rod sleeve to move along the inner wall of the outer magnetic rod sleeve away from the first end of the outer magnetic rod sleeve, so that: the fourth sample liquid is extracted into the outer magnetic rod sleeve;

[0148] Adding liquid: when the outer magnetic rod sleeve of the magnetic rod assembly in the second assembly state contains the fifth sample liquid, driving the inner magnetic rod sleeve to move along the inner wall of the outer magnetic rod sleeve close to the first end of the outer magnetic rod sleeve, so that: the fifth sample liquid is released from the outer magnetic rod sleeve;

[0149] Separating the magnetic beads and sucking out the liquid at the same time: inserting the first end of the magnetic bar assembly in the first assembly state into the sixth sample liquid containing the fourth magnetic beads, driving the inner magnetic bar sleeve to move along the inner wall of the outer magnetic bar sleeve away from the first end of the outer magnetic bar sleeve, so that: the sixth sample liquid is extracted into the outer magnetic bar sleeve, and the fourth magnetic beads are adsorbed outside the inner magnetic bar sleeve;

[0150] Adding liquid while separating magnetic beads: when the magnetic bar assembly in the first assembly state has fifth magnetic beads adsorbed, and the outer magnetic bar sleeve has seventh sample liquid, driving the inner magnetic bar sleeve to carry the magnetic bar along the inner wall of the outer magnetic bar sleeve and move close to the first end of the outer magnetic bar sleeve, so that: the magnetic bar assembly adsorbs the fifth magnetic beads and releases the seventh sample liquid without magnetic beads to the outside of the outer magnetic bar sleeve;

[0151] Mixing of liquid: inserting the first end of the magnetic rod assembly in the second assembly state into the eighth sample liquid, and driving the magnetic rod assembly to perform stirring motion in the eighth sample liquid, so that: the eighth sample liquid is mixed.

[0152] Among them, the fourth sample liquid, the fifth sample liquid, the sixth sample liquid, the seventh sample liquid, and the eighth sample liquid can be the same sample liquid or different sample liquids. Correspondingly, the fourth magnetic beads and the fifth magnetic beads can be understood as magnetic beads in the same sample liquid or as magnetic beads in different sample liquids.

[0153] Furthermore, the aspirating of liquid, the adding of liquid, the aspirating of liquid while separating magnetic beads, the adding of liquid while separating magnetic beads, and the mixing of liquid can be separate operating methods or can be any combination of the operating methods to form an integrated operating method.

[0154] In one example operation process, the liquid may be first sucked out, then added, and then mixed, thereby completing the process of transferring one sample liquid to another sample liquid and mixing it. In another example operation process, the liquid may be first sucked out while separating the magnetic beads, then added while separating the magnetic beads, and then mixed. Thus, the liquid (not containing magnetic beads) in a sample liquid containing magnetic beads may be transferred to another sample liquid.

[0155] In other examples, the liquid can be sucked out alone, the liquid can be added alone, the liquid can be sucked out while separating the magnetic beads alone, the liquid can be added while separating the magnetic beads alone, or the liquid can be mixed alone. The liquid mixing can be the mixing of the liquid without magnetic beads or the mixing of the liquid with magnetic beads.

[0156] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A magnetic bar assembly, characterized in that: It includes a magnetic rod, an inner magnetic rod sleeve and an outer magnetic rod sleeve; The inner layer magnetic rod sleeve and the outer layer magnetic rod sleeve each include a hollow rod sleeve body with two ends; wherein: The first end of the two ends of the inner magnetic rod sleeve is closed, and the second end is open; the cross-sectional size of at least part of the outer wall of the magnetic rod is smaller than or equal to the cross-sectional size of the inner wall of the inner magnetic rod sleeve, and the magnetic rod can be selectively inserted into the inner magnetic rod sleeve from the second end of the inner magnetic rod sleeve or separated from the inner magnetic rod sleeve; The first end and the second end of the two ends of the outer magnetic rod sleeve are both open; the cross-sectional size of the inner wall of the outer magnetic rod sleeve is closely matched with the cross-sectional size of at least part of the outer wall of the inner magnetic rod sleeve, so that the inner magnetic rod sleeve can be inserted into the outer magnetic rod sleeve from the second end of the outer magnetic rod sleeve and reciprocate along the inner wall of the outer magnetic rod sleeve, and the sample liquid can be extracted into the outer magnetic rod sleeve or released to the outside of the outer magnetic rod sleeve during the reciprocating motion; Wherein, the length of the magnetic rod is greater than the length of the inner magnetic rod sleeve; the length of the inner magnetic rod sleeve is greater than the length of the outer magnetic rod sleeve; The assembly state of the magnetic bar assembly includes a first assembly state and a second assembly state; When in the first assembly state, the magnetic bar is inserted into the inner magnetic bar sleeve, and the inner magnetic bar sleeve is inserted into the outer magnetic bar sleeve; In the second assembly state, the inner magnetic rod sleeve is inserted into the outer magnetic rod sleeve, and the magnetic rod is not inserted into the inner magnetic rod sleeve.

2. The magnetic bar assembly according to claim 1, characterized in that: When in the first assembly state, the magnetic bar assembly is used to: When the magnetic rod is inserted into the first end portion contacting the inner magnetic rod sleeve, the first end portion of the inner magnetic rod sleeve is inserted into the outer magnetic rod sleeve, and the first end portion of the outer magnetic rod sleeve is inserted into the sample solution containing magnetic beads, the magnetic beads in the sample solution are adsorbed onto the outer side of the inner magnetic rod sleeve and / or the outer side of the outer magnetic rod sleeve.

3. The magnetic bar assembly according to claim 1, characterized in that: The magnetic bar assembly is used for: When the magnetic bar assembly in the first assembly state absorbs magnetic beads and the magnetic bar is drawn out of the inner magnetic bar sleeve or loses its magnetic force, the magnetic beads fall off the magnetic bar assembly.

4. The magnetic bar assembly according to claim 1, characterized in that: When in the first assembly state, the magnetic bar assembly is used to: When a sample liquid containing magnetic beads is inserted into the first end of the outer magnetic rod sleeve and the inner magnetic rod sleeve moves along the inner wall of the outer magnetic rod sleeve away from the first end of the outer magnetic rod sleeve, the sample liquid is extracted into the outer magnetic rod sleeve, and the magnetic beads in the sample liquid are adsorbed on the outer side of the inner magnetic rod sleeve.

5. The magnetic bar assembly according to claim 1, characterized in that: When in the first assembly state, the magnetic bar assembly is used to: Magnetic beads are adsorbed on the outside of the inner magnetic rod sleeve, and sample liquid is contained in the outer magnetic rod sleeve. When the inner magnetic rod sleeve carries the magnetic rod and moves along the inner wall of the outer magnetic rod sleeve close to the first end of the outer magnetic rod sleeve, the sample liquid without the magnetic beads is released to the outside of the outer magnetic rod sleeve.

6. The magnetic bar assembly according to claim 1, characterized in that: When in the second assembly state, the magnetic bar assembly is used to: insert the sample liquid into the first end of the outer magnetic rod sleeve, and when the inner magnetic rod sleeve moves along the inner wall of the outer magnetic rod sleeve away from the first end of the outer magnetic rod sleeve, the sample liquid is extracted into the outer magnetic rod sleeve.

7. The magnetic bar assembly according to claim 1, characterized in that: When in the second assembly state, the magnetic bar assembly is used to: The outer magnetic rod sleeve contains sample liquid, and when the inner magnetic rod sleeve moves along the inner wall of the outer magnetic rod sleeve and approaches the first end of the outer magnetic rod sleeve, the sample liquid is released to the outside of the outer magnetic rod sleeve.

8. The magnetic bar assembly according to claim 1, characterized in that: When in the second assembly state, the magnetic bar assembly is used to: The sample liquid is inserted into the first end of the outer magnetic rod sleeve, and when the outer magnetic rod sleeve is operated to perform stirring motion in the sample liquid, the sample liquid is mixed.

9. The magnetic bar assembly according to claim 1, characterized in that: The cross-sectional shape of the magnetic bar is at least one of the following: Round; Polygon; Oval.

10. The magnetic bar assembly according to claim 1, characterized in that: The cross-sectional shape of the magnetic bar matches the cross-sectional shape of the inner wall of the inner magnetic bar sleeve.

11. The magnetic bar assembly according to claim 1, characterized in that: The cross-sectional shape of the inner magnetic rod sleeve and the cross-sectional shape of the outer magnetic rod sleeve are both circular; the cross-sectional shape of the outer wall of the inner magnetic rod sleeve and the cross-sectional shape of the inner wall of the outer magnetic rod sleeve are both circular.

12. The magnetic bar assembly according to claim 1, characterized in that: A pull rod is provided at one end of the magnetic bar.

13. The magnetic bar assembly according to claim 1, characterized in that: The material of the magnetic bar includes a permanent magnet or an electromagnet.

14. The magnetic bar assembly according to claim 13, characterized in that: When the material of the magnetic bar is an electromagnet, the magnetic bar assembly further includes an electromagnetic control device, and the electromagnetic control device is electrically connected to the electromagnet.

15. A device for extracting nucleic acid, characterized in that: A magnetic bar assembly comprising any one of claims 1 to 14.

16. A method for treating liquid using the nucleic acid extraction device according to claim 15, characterized in that: Including processing of magnetic beads and / or processing of liquid; the assembly state of the magnetic bar assembly includes a first assembly state and a second assembly state; the first end of the magnetic bar assembly is the first end of the outer magnetic bar sleeve; in: The processing of the magnetic beads includes at least one of the following: Separation of magnetic beads: inserting the first end of the magnetic rod assembly in the first assembly state into a first sample solution containing magnetic beads to adsorb the first magnetic beads in the first sample solution; Transfer of magnetic beads: when the magnetic bar assembly in the first assembly state absorbs the second magnetic beads, the magnetic bar is withdrawn from the inner magnetic bar sleeve or the magnetic bar is controlled to lose its magnetic force, so that: the second magnetic beads fall off from the magnetic bar assembly; Mixing the magnetic beads: inserting the first end of the magnetic bar assembly in the second assembly state into a third sample solution containing third magnetic beads, and driving the magnetic bar assembly to perform stirring motion in the third sample solution, so that: the third magnetic beads in the third sample solution are mixed; The treatment of the liquid includes at least one of the following: Liquid aspiration: inserting the first end of the magnetic rod assembly in the second assembly state into the fourth sample liquid, driving the inner magnetic rod sleeve to move along the inner wall of the outer magnetic rod sleeve away from the first end of the outer magnetic rod sleeve, so that: the fourth sample liquid is extracted into the outer magnetic rod sleeve; Adding liquid: when the outer magnetic rod sleeve of the magnetic rod assembly in the second assembly state contains the fifth sample liquid, driving the inner magnetic rod sleeve to move along the inner wall of the outer magnetic rod sleeve close to the first end of the outer magnetic rod sleeve, so that: the fifth sample liquid is released from the outer magnetic rod sleeve; Separating the magnetic beads and sucking out the liquid at the same time: inserting the first end of the magnetic bar assembly in the first assembly state into the sixth sample liquid containing the fourth magnetic beads, driving the inner magnetic bar sleeve to move along the inner wall of the outer magnetic bar sleeve away from the first end of the outer magnetic bar sleeve, so that: the sixth sample liquid is extracted into the outer magnetic bar sleeve, and the fourth magnetic beads are adsorbed outside the inner magnetic bar sleeve; Adding liquid while separating magnetic beads: when the magnetic rod assembly in the first assembly state adsorbs fifth magnetic beads and the outer magnetic rod sleeve contains the seventh sample liquid, the inner magnetic rod sleeve is driven to carry the magnetic rod along the inner wall of the outer magnetic rod sleeve and move close to the first end of the outer magnetic rod sleeve, so that: the magnetic rod assembly adsorbs the fifth magnetic beads and releases the seventh sample liquid without magnetic beads to the outside of the outer magnetic rod sleeve; mixing the liquid: inserting the first end of the magnetic rod assembly in the second assembly state into the eighth sample liquid, and driving the magnetic rod assembly to stir the eighth sample liquid, so that: the eighth sample liquid is mixed.

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