Multi-channel fluorescence quantitative detection device and molecular diagnostic platform

Abstract

The present application relates to the field of fluorescence detection technology, and in particular to a multi-channel fluorescence quantitative detection device and a molecular diagnostic platform. The fluorescence quantitative detection device includes a light source component, a first filter component, a second filter component, an imaging component, an optical fiber bundle and an optical detection slot. The optical detection slot is used to fix a plurality of capillaries; the light source component can provide a white light source, and the white light source emitted by the light source component is filtered by the first filter component to form an incident light of a predetermined wavelength band, and then irradiated to the corresponding plurality of capillaries through the incident optical fibers of the plurality of optical fiber bundles to excite fluorescence. The fluorescence generated by the plurality of capillaries is filtered by the output optical fibers of the plurality of optical fiber bundles and the second filter component to form fluorescence of the corresponding wavelength band and irradiate the imaging component, so as to conveniently and quickly obtain fluorescence imaging photos of the plurality of capillaries through the imaging component, so as to analyze the concentration of the samples in the plurality of capillaries and improve the efficiency of fluorescence quantitative detection of the samples.

Description

Technical Field

[0001] The present application relates to the field of fluorescence detection technology, and in particular to a multi-channel fluorescence quantitative detection device and a molecular diagnosis platform. Background Art

[0002] Fluorescence quantitative detection is the detection part in the final step of molecular diagnosis. During the detection process, an excitation light of a predetermined wavelength is used to irradiate the fluorescent substance in the capillary to stimulate fluorescence, and then the fluorescence is collected by a fluorescence collection device to achieve fluorescence quantitative detection of the reactants. However, the current fluorescence quantitative detection devices have the problem of too long detection time. Summary of the invention

[0003] The object of the present invention is to provide a multi-channel fluorescence quantitative detection device and a molecular diagnosis platform to improve the fluorescence detection efficiency to a certain extent.

[0004] The present invention provides a multi-channel fluorescence quantitative detection device, comprising a frame, a light source component, a first filter component, a second filter component, an imaging component, an optical fiber bundle and a light detection slot;

[0005] The optical inspection slot is arranged on the rack, and the optical inspection slot is used to fix the capillary containing the sample; the light source assembly and the first filter assembly are arranged on the rack oppositely, and the light source assembly is used to provide a white light source, and the white light source can pass through the first filter assembly to form an incident light of a predetermined band; the optical fiber bundle includes three ports, the first port of the optical fiber bundle is connected to the first filter assembly, and the second port of the optical fiber bundle is connected to the optical inspection slot, and the incident light can be irradiated to the capillary through the incident optical fiber between the first port and the second port to excite fluorescence; the second filter assembly and the imaging assembly are arranged on the rack oppositely, and the third port of the optical fiber bundle is connected to the second filter assembly so that the fluorescence is irradiated to the second filter assembly through the outgoing optical fiber between the second port and the third port; the second filter assembly is used to filter the fluorescence so that the fluorescence of the predetermined band is irradiated into the imaging assembly, and the imaging assembly is used to collect the fluorescence and form an image;

[0006] There are multiple capillaries, which are arranged side by side and spaced apart; there are multiple optical fiber bundles, which correspond to the capillaries one by one, and the second ports of the optical fiber bundles face the corresponding capillaries respectively.

[0007] Furthermore, the first filter assembly and the second filter assembly both include a filter wheel, a filter and a driving device; the driving device is fixedly arranged on the frame, and the driving end of the driving device is connected to the filter wheel to drive the filter wheel to rotate around the axis of the filter wheel; there are multiple filters, and the multiple filters are arranged at intervals along the circumference of the filter wheel; the multiple filters of the first filter assembly are used to filter the white light source to obtain incident light of different bands; the multiple filters of the second filter assembly are used to filter the fluorescence to allow the fluorescence of a predetermined band to irradiate the imaging assembly.

[0008] Furthermore, the first filter assembly and the second filter assembly also include light shields, respectively; the light shields are arranged on the filter wheel, and the side walls on both sides of the light shield are respectively provided with a light inlet and a light outlet, and the light inlet and the light outlet are located on the same axis; the rotation of the filter wheel can rotate the plurality of filters in sequence to between the light inlet and the light outlet, so as to form an optical path channel between the light inlet and the light outlet.

[0009] Furthermore, the light source assembly includes an LED lamp, a light source sleeve and a collimating lens; the light source sleeve is fixedly arranged on the frame, and the light source sleeve is coaxially arranged with the light inlet of the first filter assembly; one end of the light source sleeve extends into the light shield through the light inlet of the first filter assembly, the LED lamp is connected to the other end of the light source sleeve, and the collimating lens is installed in the light source sleeve.

[0010] Furthermore, the light source assembly also includes a heat sink; the heat sink is installed on the frame, and the light source sleeve is used to install one end of the LED lamp and is connected to the heat sink, so that the LED lamp is clamped between the light source sleeve and the heat sink, and the aluminum substrate of the LED lamp is in contact with the heat sink.

[0011] Furthermore, the multi-channel fluorescence quantitative detection device also includes a first fiber optic lens assembly; the first fiber optic lens assembly includes a first sleeve, a first focusing lens and a first fiber optic fixing member; the first sleeve is fixedly arranged on the frame, and the first sleeve is coaxially arranged with the light outlet of the first filter assembly; one end of the first sleeve extends into the light shield of the first filter assembly through the light outlet of the first filter assembly, and the other end of the first sleeve is connected to the first fiber optic fixing member; the first port of the fiber optic bundle is inserted into the first sleeve through the first fiber optic fixing member, the first focusing lens is installed in the first sleeve, and the first port of the fiber optic bundle is located on the focal plane of the first focusing lens.

[0012] Furthermore, the multi-channel fluorescence quantitative detection device also includes a second fiber optic lens assembly; the second fiber optic lens assembly includes a second sleeve, a second lens and a second fiber optic fixing piece; the second sleeve is fixedly arranged on the frame, and the second sleeve is coaxially arranged with the light inlet of the second filter assembly; one end of the second sleeve extends into the light shield of the second filter assembly through the light inlet of the second filter assembly, and the other end of the second sleeve is connected to the second fiber optic fixing piece; the third port of the fiber optic bundle is plugged into the second sleeve through the second fiber optic fixing piece, and the second lens is installed in the second sleeve; the fluorescence emitted by the third port of the fiber optic bundle can be irradiated onto the filter of the second filter assembly located in the working position via the second lens.

[0013] Furthermore, the imaging assembly includes a camera, a first lens and a camera lens sleeve; the first lens is installed in the camera lens sleeve, the camera lens sleeve is fixedly arranged on the frame, and the camera lens sleeve is coaxially arranged with the light outlet of the second filter assembly, and one end of the camera lens sleeve extends into the light shield of the second filter assembly through the light outlet of the second filter assembly; the camera is fixedly arranged on the frame, and the other end of the camera lens sleeve is connected to the camera so that the fluorescence passing through the second filter assembly is irradiated onto the CMOS chip of the camera.

[0014] Furthermore, the optical inspection groove is formed with a plurality of detection chambers arranged side by side and spaced apart, and the plurality of detection chambers correspond one-to-one to the plurality of capillaries; a silicone cover is provided at the open end of the optical inspection groove, and a capillary plugging hole is provided on the silicone cover at a position corresponding to each of the detection chambers, so that the capillary can be plugged into the corresponding detection chamber; a plurality of light-through holes are provided on the side wall of the optical inspection groove, and the plurality of light-through holes correspond one-to-one to and are connected with the plurality of detection chambers; an optical fiber fixing plate is installed on the side wall of the optical inspection groove, and a plurality of optical fiber fixing holes are provided on the optical fiber fixing plate corresponding to the plurality of light-through holes, and each of the optical fiber fixing holes and the corresponding light-through hole are located on the same axis; a second focusing lens is respectively clamped between each of the light-through holes and each of the corresponding light-through holes; and the second ports of the plurality of optical fiber bundles are respectively plugged into the plurality of optical fiber fixing holes.

[0015] Furthermore, a heating plate is provided on the bottom wall of the optical inspection slot for heating the optical inspection slot.

[0016] Furthermore, the optical inspection tank is also formed with a first heat preservation chamber and a second heat preservation chamber, and the plurality of inspection chambers are located between the first heat preservation chamber and the second heat preservation chamber; and a heat preservation layer is arranged outside the optical inspection tank to keep the optical inspection tank warm.

[0017] The present invention also provides a molecular diagnostic platform, comprising any of the multi-channel fluorescence quantitative detection devices described above.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The fluorescence quantitative detection device provided by the present invention comprises a frame, a light source assembly, a first filter assembly, a second filter assembly, an imaging assembly, an optical fiber bundle and an optical inspection slot. The optical inspection slot is placed on the frame, and is used to fix a plurality of capillaries containing samples to be detected. The light source assembly can provide a white light source, and the light source assembly and the first filter assembly are arranged on the frame opposite to each other. The white light source emitted by the light source assembly can be irradiated on the first filter assembly, and then the white light source is filtered by the first filter assembly, so that monochromatic light of a predetermined wavelength band passes through the first filter assembly to form an incident light with a predetermined wavelength band for irradiating the capillary. There are multiple optical fiber bundles, and the multiple optical fiber bundles correspond to the multiple capillaries one by one. The first ports of the multiple optical fiber bundles converge into one and are connected to the first filter assembly. The incident light formed by the first filter assembly can enter the multiple incident optical fibers uniformly through the multiple first ports. The second ports of the multiple optical fiber bundles are respectively connected to the optical inspection grooves, and the multiple second ports are respectively facing the corresponding capillaries, so that the incident light is irradiated on the corresponding capillaries through the second ports, thereby stimulating the fluorescent substances in the capillaries to emit fluorescence of the corresponding band. The second filter component and the imaging component are relatively arranged on the rack, and the third ports of the multiple optical fiber bundles are converged into one and connected to the first filter component, so that the fluorescence of the corresponding band emitted by the multiple capillaries can be irradiated on the second filter component, and after the stray light is filtered out by the second filter component, it is irradiated on the imaging component, so that the fluorescence imaging photos of the multiple capillaries can be obtained conveniently and quickly through the imaging component to analyze the concentration of the sample and improve the efficiency of the fluorescence quantitative detection of the sample.

[0020] The present invention also provides a molecular diagnosis platform, including the multi-channel fluorescence quantitative detection device, so the molecular diagnosis platform also has the beneficial effects of the multi-channel fluorescence quantitative detection device. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0022] Figure 1 A schematic diagram of the structure of a multi-channel fluorescence quantitative detection device provided by an embodiment of the present invention at a first viewing angle;

[0023] Figure 2 A schematic diagram of the structure of a multi-channel fluorescence quantitative detection device provided by an embodiment of the present invention under a second viewing angle;

[0024] Figure 3 for Figure 2 Cross-section at AA;

[0025] Figure 4 for Figure 2 Cross-section at BB;

[0026] Figure 5 A schematic diagram of the structure of an optical inspection cell provided in an embodiment of the present invention.

[0027] Reference numerals:

[0028] 1- rack;

[0029] 2-light source assembly, 21-LED lamp, 22-light source sleeve, 23-collimating lens, 24-heat sink;

[0030] 3-first filter assembly, 31-filter wheel, 32-filter, 33-driving device, 34-light shield, 35-sensing sheet, 36-photoelectric sensor;

[0031] 4- second filter component;

[0032] 5-imaging assembly, 51-camera, 52-camera lens sleeve, 53-first lens;

[0033] 6-first optical fiber lens assembly, 61-first sleeve, 62-first focusing lens, 63-first optical fiber fixing member;

[0034] 7-second optical fiber lens assembly, 71-second sleeve, 72-second lens, 73-second optical fiber fixing member;

[0035] 8-optical inspection groove, 81-light through hole, 82-optical fiber fixing plate, 83-optical fiber fixing hole, 84-silicone cover plate. DETAILED DESCRIPTION

[0036] The technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0037] The components of the embodiments of the present invention generally described and shown in the drawings herein may be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention.

[0038] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0039] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0040] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0041] Refer to the following Figures 1 to 5 The multi-channel fluorescence quantitative detection device and molecular diagnosis platform according to some embodiments of the present application are described.

[0042] The present application provides a fluorescence quantitative detection device for performing fluorescence quantitative detection on a sample in a capillary; Figure 1 As shown, the fluorescence quantitative detection device includes a frame 1, a light source component 2, a first filter component 3, a second filter component 4, an imaging component 5, an optical fiber bundle and a light detection slot 8.

[0043] The optical inspection slot 8 is placed on the frame 1 and is used to fix the capillary tube containing the sample to be detected. There are multiple capillaries, which are arranged side by side and spaced apart in the optical inspection slot 8.

[0044] The light source assembly 2 can provide a white light source. The light source assembly 2 and the first filter assembly 3 are arranged on the frame 1 opposite to each other. The white light source emitted by the light source assembly 2 can be irradiated on the first filter assembly 3, and then the white light source is filtered by the first filter assembly 3, so that monochromatic light of a predetermined band passes through the first filter assembly 3, forming an incident light with a predetermined band for irradiating the capillary.

[0045] The optical fiber bundle is a three-port optical fiber bundle, including a first port, a second port and a third port, an incident optical fiber is formed between the first port and the second port, and an exit optical fiber is formed between the second port and the third port. There are multiple optical fiber bundles, and the multiple optical fiber bundles correspond to the multiple capillaries one by one. The first ports of the multiple optical fiber bundles converge into one and are connected to the first filter component 3. The incident light formed by the first filter component 3 can evenly enter the multiple incident optical fibers through the multiple first ports.

[0046] The second ports of the multiple optical fiber bundles are respectively connected to the optical inspection slot 8, and the multiple second ports are respectively directed to the corresponding capillaries. The incident light is transmitted to the corresponding second port via the multiple incident optical fibers, and then the incident light of the predetermined wavelength band is irradiated on the corresponding capillary through the second port, thereby exciting the fluorescent material in the capillary to emit fluorescence of the corresponding wavelength band.

[0047] The second filter component 4 is arranged on the frame 1, and the third ports of the multiple optical fiber bundles are converged into one and connected to the second filter component 4, so that the fluorescence of the corresponding bands emitted by the multiple capillaries can be irradiated on the second filter component 4; the imaging component 5 is arranged opposite to the second filter component 4, and the fluorescence can be irradiated on the imaging component 5 through the second filter component 4, and the stray light is removed by the second filter component 4, so that the fluorescence imaging photos of the multiple capillaries can be obtained conveniently and quickly through the imaging component 5 to analyze the concentration of the sample and improve the efficiency of the fluorescence quantitative detection of the sample.

[0048] In one embodiment of the present application, preferably, Figures 1 to 3 As shown, the first filter component 3 can filter the white light source emitted by the light source component 2 to form a variety of monochromatic lights with different wavelengths.

[0049] Preferably, if Figure 3 As shown, the first filter assembly 3 includes a filter wheel 31, a filter 32, a driving device 33 and a light shield 34; the driving device 33 is fixedly arranged on the frame 1, and the driving end of the driving device 33 is connected to the filter wheel 31, so as to drive the filter wheel 31 to rotate around the axis of the filter wheel 31 through the driving device 33. There are multiple filters 32, and the multiple filters 32 are spaced along the circumference of the filter wheel 31, and the multiple filters 32 can respectively allow light of different wavelength bands to pass through. The light shield 34 is arranged on the filter wheel 31, and a light inlet is provided on the side wall of the light shield 34 on the side opposite to the filter wheel 31, and a light outlet is provided on the side wall of the other side of the light shield 34, and the light inlet and the light outlet are located on the same axis. The driving device 33 can drive the filter wheel 31 to rotate, so that the multiple filters 32 rotate in sequence to between the light inlet and the light outlet, and are located on the same axis as the light inlet and the light outlet, thereby forming an optical path channel between the light inlet and the light outlet.

[0050] The white light source emitted by the light source assembly 2 can be irradiated onto the filter 32 located between the light inlet and the light outlet through the light inlet, and then the white light source is filtered by the filter 32, so that the monochromatic light of the corresponding band passes through the filter 32 and is emitted through the light outlet to form an incident light with a predetermined band for irradiating the capillary.

[0051] In this embodiment, preferably, the structure of the second filter component 4 is the same as that of the first filter component 3, which will not be repeated here; the number of filters on the second filter component 4 is the same as the number of filters 32 on the first filter component 3 and corresponds one to one.

[0052] Preferably, the number of filters 32 on the first filter assembly 3 is four, and the four filters 32 are respectively a red filter, a yellow filter, a green filter and a blue filter; the red filter is used to allow red light of 607-644nm to pass through, the yellow filter is used to allow yellow light of 552-594nm to pass through, the green filter is used to allow green light of 509-545nm to pass through, and the blue filter is used to allow blue light of 420-490nm to pass through.

[0053] When the incident light of the four wavelength bands is irradiated on the capillary, the fluorescent substance in the capillary can excite the fluorescence of the corresponding wavelength bands, which are red fluorescence, yellow fluorescence, green fluorescence and blue fluorescence. Correspondingly, the number of filters on the second filter component 4 is also four, and the four filters are red fluorescence filter, yellow fluorescence filter, green fluorescence filter and blue fluorescence filter; the red fluorescence filter is used to allow the red fluorescence of 644-686nm to pass through, the yellow fluorescence filter is used to allow the yellow fluorescence of 594-634nm to pass through, the green fluorescence filter 32 is used to allow the green fluorescence of 545-583nm to pass through, and the blue fluorescence filter is used to allow the blue fluorescence of 490-532nm to pass through.

[0054] In the specific use process, taking red incident light as an example, when it is necessary to use red incident light to irradiate the capillary, the red filter and the red fluorescent filter are respectively rotated to the corresponding optical path channels, so that the white light source emitted by the light source is filtered by the first filter component 3 to form an incident light of 607-644nm, and is covered on the capillary, and then the fluorescent substance in the capillary is excited to emit red fluorescence; the red fluorescence is filtered by the second filter component 4, so that the 644-686nm red fluorescence is irradiated into the imaging component 5, and the imaging component 5 obtains the fluorescent imaging photos formed by multiple capillaries under the excitation of the 607-644nm incident light.

[0055] In one embodiment of the present application, preferably, the driving devices 33 of the first filter assembly 3 and the second filter assembly 4 are both stepper motors, so that the rotation angle of the corresponding filter wheel 31 can be accurately controlled by the stepper motor to rotate the required filter 32 to the optical path channel.

[0056] Preferably, if Figure 1 As shown, photoelectric sensors 36 are also provided at the stepper motors of the first filter component 3 and the second filter component 4 respectively; one end of the output shaft of the stepper motor is connected to the filter wheel 31, and the other end of the output shaft is provided with a sensor plate 35, so that the sensor plate 35 can rotate synchronously with the filter wheel 31 at the same angle.

[0057] The edge of the sensing sheet 35 can extend into the photoelectric sensor 36, and a notch is provided on the edge of the sensing sheet 35. When the sensing sheet 35 is rotated until the notch is located in the sensing area of ​​the photoelectric sensor 36, the filter wheel 31 is in the initial position; then, based on the initial position, the filter wheel is driven by a stepper motor to rotate at different angles, so that the four filters 32 can be rotated to the optical path in sequence.

[0058] In one embodiment of the present application, preferably, Figure 3 As shown, the light source assembly 2 includes an LED lamp 21, a light source sleeve 22, a collimating lens 23 and a heat sink 24. The light source sleeve 22 is fixedly arranged on the frame 1, and the light source sleeve 22 is located on the side of the first filter assembly 3 where the light inlet is opened; the light source sleeve 22 is coaxially arranged with the light inlet of the first filter assembly 3, and one end of the light source sleeve 22 away from the first filter assembly 3 is connected to the LED lamp 21, and the other end of the light source sleeve 22 extends into the light shield 34 through the light inlet of the first filter assembly 3, and extends to a position close to the filter wheel 31. The collimating lens 23 is installed in the light source sleeve 22, and the LED lamp 21 can emit white light, and the white light emitted by the LED forms a beam of parallel light after being corrected by the collimating lens 23; the parallel light irradiates the filter 32 located on the optical path, and then forms incident light of a predetermined wavelength band through the filtration of the filter 32, so as to excite the fluorescent substance in the capillary.

[0059] In one embodiment of the present application, preferably, Figure 3 As shown, a first optical fiber lens assembly 6 is also provided on one side of the first filter assembly 3 where a light outlet is provided; the first optical fiber lens assembly 6 includes a first sleeve 61 , a first focusing lens 62 and a first optical fiber fixing member 63 .

[0060] The first sleeve 61 is fixedly arranged on the frame 1, and the first sleeve 61 is coaxially arranged with the light outlet of the first filter assembly 3; one end of the first sleeve 61 extends into the light shield 34 of the first filter assembly 3 through the light outlet of the first filter assembly 3 and extends to the filter wheel 31, and the other end of the first sleeve 61 is connected to the first optical fiber fixing member 63; the first ends of the plurality of optical fiber bundles are converged into one and then inserted into the first sleeve 61 through the first optical fiber fixing member 63, the first focusing lens 62 is installed in the first sleeve 61, and the first end of the optical fiber bundle is located on the focal plane of the first focusing lens 62; the incident light of the predetermined wavelength band obtained after filtering by the first filter assembly 3 can be uniformly dispersed into the first ends of the plurality of optical fiber bundles after being focused by the first focusing lens 62, and then transmitted to the corresponding second ends through the incident optical fibers of the plurality of optical fiber bundles, and then the incident light of the predetermined wavelength band is irradiated on the corresponding capillaries through the second ends, thereby exciting the fluorescent substances in the plurality of capillaries to generate fluorescence of the corresponding wavelength band.

[0061] In one embodiment of the present application, preferably, Figure 4 As shown, a second optical fiber lens assembly 7 is arranged on one side of the second optical filter assembly 4 where a light inlet is provided, and the second optical fiber lens assembly 7 comprises a second sleeve 71, a second lens 72 and a second optical fiber fixing member 73. The second sleeve 71 is fixedly arranged on the frame 1, and the second sleeve 71 is coaxially arranged with the light inlet of the second optical filter assembly 4; one end of the second sleeve 71 extends into the light shield of the second optical filter assembly 4 through the light inlet of the second optical filter assembly 4 and extends to the filter wheel of the second optical filter assembly 4, and the other end of the second sleeve 71 is connected to the second optical fiber fixing member 73. The third ports of the multiple optical fiber bundles converge into one and are inserted into the second sleeve 71 through the second optical fiber fixing piece 73, and the second lens 72 is installed in the second sleeve 71; the fluorescence emitted by the multiple capillaries can enter the output optical fibers of the multiple optical fiber bundles through the second ports of the corresponding optical fiber bundles, and then be transmitted to the third ports of the multiple optical fiber bundles through the multiple output optical fibers, and the fluorescence emitted by the capillaries is irradiated on the second lens 72 through the multiple third ports; the second lens 72 is used to correct the fluorescent light to obtain a parallel fluorescent light beam, so that the parallel fluorescent light beam is irradiated on the filter of the second filter component 4, so as to filter the fluorescent light beam through the filter of the second filter component 4, so that the fluorescent light beam of the predetermined wavelength band is irradiated into the imaging component 5 to obtain a fluorescent imaging photo.

[0062] In one embodiment of the present application, preferably, Figure 1 and Figure 4As shown, the imaging component 5 is arranged on a side of the second filter component 4 where a light outlet is provided, and the imaging component 5 includes a camera 51, a first lens 53 and a camera lens sleeve 52, wherein the first lens 53 is installed in the camera lens sleeve 52, and the first lens 53 and the camera lens sleeve 52 are coaxially arranged; the camera lens sleeve 52 is fixedly arranged on the frame 1, and the camera lens sleeve 52 and the light outlet of the second filter component 4 are coaxially arranged, and one end of the camera lens sleeve 52 extends into the light shield of the second filter component 4 through the light outlet of the second filter component 4 and extends to the filter wheel. Preferably, the first lens 53 and the second lens 72 on both sides of the second filter component 4 are both achromatic doublet lenses; the camera 51 is fixedly arranged on the frame 1, and the other end of the camera lens sleeve 52 is connected to the camera 51, so that the fluorescence emitted from the third port of the optical fiber bundle is sequentially irradiated into the CMOS chip of the camera 51 through the second lens 72, the second filter component 4 and the first lens 53, thereby, the fluorescence signal can be collected by the camera 51 and the fluorescence imaging photo can be obtained without the need for a camera lens.

[0063] In one embodiment of the present application, preferably, Figure 5 As shown, the optical inspection slot 8 is formed with a plurality of independent detection chambers, the plurality of detection chambers are arranged side by side and spaced apart, and the plurality of detection chambers correspond one to one with the plurality of capillaries. The upper end of the optical inspection slot 8 is an open end, and the open end is covered with a silicone cover plate 84, so that the plurality of detection chambers are sealed by the silicone cover plate 84. The silicone cover plate 84 is provided with a capillary plug hole at the position corresponding to each detection chamber, so that the plurality of capillaries can be inserted into the corresponding detection chamber through the capillary plug hole.

[0064] A light-through hole 81 is provided on the side wall of one side of the optical inspection slot 8 at the position corresponding to each detection chamber, and the light-through hole 81 is directly opposite to the end of the capillary tube section containing the sample. An optical fiber fixing plate 82 is provided on the side wall of the optical inspection slot 8 on the side where the light-through hole 81 is provided, and a plurality of optical fiber fixing holes 83 are provided on the optical fiber fixing plate 82. The plurality of optical fiber fixing holes 83 correspond to the plurality of light-through holes 81 one by one, and a corresponding group of light-through holes 81 and optical fiber fixing holes 83 are located on the same axis. The second ports of the plurality of optical fiber bundles are respectively plugged into the plurality of optical fiber fixing holes 83, and a second focusing lens is provided between each group of light-through holes 81 and optical fiber fixing holes 83, so that the incident light emitted from the second port of the optical fiber bundle is focused by the corresponding second focusing lens and then irradiated on the corresponding capillary tube to excite the fluorescent substance in the capillary tube to emit fluorescence.

[0065] In this embodiment, preferably, a heating plate is provided on the bottom wall of the optical detection slot 8, and the optical detection slot 8 can be heated by the heating plate to keep the detection chamber inside the optical detection slot 8 at a suitable temperature, thereby ensuring the accuracy of fluorescence quantitative detection.

[0066] Preferably, in order to further ensure that the multiple detection chambers are at the same temperature, a first insulation chamber and a second insulation chamber are also formed in the optical inspection groove 8. The first insulation chamber is located at one end of the multiple detection chambers, and the second insulation chamber is located at the other end of the multiple detection chambers, thereby avoiding the two detection chambers located at both ends of the multiple detection chambers from being too cold.

[0067] Preferably, a heat-insulating layer is further provided outside the optical detection tank 8 to further insulate the multiple detection chambers, ensure that the temperature inside the optical detection tank 8 is within a stable range, and provide accuracy for fluorescence quantitative detection.

[0068] The present application also provides a molecular diagnostic platform, comprising the multi-channel fluorescence quantitative detection device of any of the above embodiments.

[0069] In this embodiment, the molecular diagnosis platform includes a multi-channel fluorescence quantitative detection device, so the molecular diagnosis platform has all the beneficial effects of the multi-channel fluorescence quantitative detection device, which will not be described in detail here.

[0070] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-channel fluorescence quantitative detection device, characterized in that: It includes a frame, a light source component, a first filter component, a second filter component, an imaging component, an optical fiber bundle and a light inspection slot; The optical inspection slot is arranged on the frame, and the optical inspection slot is used to fix the capillary tube containing the sample; The light source assembly and the first filter assembly are arranged on the frame opposite to each other, and the light source assembly is used to provide a white light source, and the white light source can pass through the first filter assembly to form incident light of a predetermined wavelength band; The optical fiber bundle comprises three ports, a first port of the optical fiber bundle is connected to the first filter assembly, a second port of the optical fiber bundle is connected to the optical inspection slot, and the incident light can be irradiated to the capillary through the incident optical fiber between the first port and the second port to excite fluorescence; The second filter assembly and the imaging assembly are arranged on the frame relative to each other, and the third port of the optical fiber bundle is connected to the second filter assembly, so that the fluorescence is irradiated to the second filter assembly through the output optical fiber between the second port and the third port; The second filter component is used to filter the fluorescence so that the fluorescence in a predetermined wavelength band can be irradiated into the imaging component, and the imaging component is used to collect the fluorescence and form an image; There are multiple capillaries, which are arranged side by side and spaced apart; there are multiple optical fiber bundles, which correspond to the capillaries one by one, and the second ports of the optical fiber bundles face the corresponding capillaries respectively.

2. The multi-channel fluorescence quantitative detection device according to claim 1, characterized in that: The first filter assembly and the second filter assembly both include a filter wheel, a filter, and a driving device; The driving device is fixedly arranged on the frame, and the driving end of the driving device is connected to the filter wheel to drive the filter wheel to rotate around the axis of the filter wheel; There are multiple filters, and the multiple filters are arranged at intervals along the circumference of the filter wheel; The multiple filters of the first filter assembly are used to filter the white light source to obtain incident light of different wavelength bands; The multiple filters of the second filter component are used to filter the fluorescence so that the fluorescence in a predetermined wavelength band can be irradiated into the imaging component.

3. The multi-channel fluorescence quantitative detection device according to claim 2, characterized in that: The first filter assembly and the second filter assembly further include light shields respectively; The light shield is arranged on the filter wheel, and the side walls on both sides of the light shield are respectively provided with a light inlet and a light outlet, and the light inlet and the light outlet are located on the same axis; The filter wheel can rotate to rotate the plurality of filters in sequence to between the light inlet and the light outlet, so as to form an optical path channel between the light inlet and the light outlet.

4. The multi-channel fluorescence quantitative detection device according to claim 3, characterized in that: The light source assembly includes an LED lamp, a light source sleeve and a collimating lens; The light source sleeve is fixedly arranged on the frame, and the light source sleeve is coaxially arranged with the light inlet of the first filter assembly; One end of the light source sleeve extends into the light shield through the light inlet of the first filter assembly, the LED lamp is connected to the other end of the light source sleeve, and the collimating lens is installed in the light source sleeve.

5. The multi-channel fluorescence quantitative detection device according to claim 4, characterized in that: The light source assembly also includes a heat sink; The heat sink is mounted on the frame, and one end of the light source sleeve for mounting the LED lamp is connected to the heat sink so that the LED lamp is clamped between the light source sleeve and the heat sink, and the aluminum substrate of the LED lamp fits the heat sink.

6. The multi-channel fluorescence quantitative detection device according to claim 3, characterized in that: Also included is a first fiber optic lens assembly; The first fiber lens assembly includes a first sleeve, a first focusing lens and a first fiber fixture; The first sleeve is fixedly arranged on the frame, and the first sleeve is coaxially arranged with the light outlet of the first filter assembly; one end of the first sleeve extends into the light shield of the first filter assembly through the light outlet of the first filter assembly, and the other end of the first sleeve is connected to the first optical fiber fixing member; The first port of the optical fiber bundle is inserted into the first sleeve through the first optical fiber fixing member, the first focusing lens is installed in the first sleeve, and the first port of the optical fiber bundle is located on the focal plane of the first focusing lens.

7. The multi-channel fluorescence quantitative detection device according to claim 3, characterized in that: Also included is a second fiber optic lens assembly; The second fiber lens assembly includes a second sleeve, a second lens, and a second fiber fixture; The second sleeve is fixedly arranged on the frame, and the second sleeve is coaxially arranged with the light inlet of the second filter assembly; one end of the second sleeve extends into the light shield of the second filter assembly through the light inlet of the second filter assembly, and the other end of the second sleeve is connected to the second optical fiber fixing member; The third port of the optical fiber bundle is inserted into the second sleeve through the second optical fiber fixing member, and the second lens is installed in the second sleeve; The fluorescence emitted from the third port of the optical fiber bundle can be irradiated onto the filter of the second filter component located at the working position through the second lens.

8. The multi-channel fluorescence quantitative detection device according to claim 3, characterized in that: The imaging assembly includes a camera, a first lens, and a camera lens tube; The first lens is installed in the camera lens sleeve, the camera lens sleeve is fixedly arranged on the frame, and the camera lens sleeve is coaxially arranged with the light outlet of the second filter assembly, and one end of the camera lens sleeve extends into the light shield of the second filter assembly through the light outlet of the second filter assembly; The camera is fixedly arranged on the frame, and the other end of the camera lens sleeve is connected to the camera so that the fluorescence passing through the second filter component is irradiated onto the CMOS chip of the camera.

9. The multi-channel fluorescence quantitative detection device according to claim 1, characterized in that: The optical detection groove is formed with a plurality of detection chambers arranged side by side and spaced apart, and the plurality of detection chambers correspond one to one with the plurality of capillaries; The open end of the optical inspection slot is provided with a silicone cover plate, and the silicone cover plate is provided with a capillary plug hole at a position corresponding to each of the detection chambers, so that the capillary can be plugged into the corresponding detection chamber; A plurality of light-through holes are provided on the side wall of the optical inspection groove, and the plurality of light-through holes correspond to and are connected with the plurality of detection chambers one by one; An optical fiber fixing plate is installed on the side wall of the optical inspection slot, and a plurality of optical fiber fixing holes are opened on the optical fiber fixing plate corresponding to the plurality of light through holes, and each of the optical fiber fixing holes and the corresponding light through hole are located on the same axis; A second focusing lens is respectively clamped between each of the light through holes and each of the corresponding light through holes; and the second ports of the plurality of optical fiber bundles are respectively plugged into the plurality of optical fiber fixing holes.

10. The multi-channel fluorescence quantitative detection device according to claim 9, characterized in that: A heating plate is arranged on the bottom wall of the optical inspection slot to heat the optical inspection slot.

11. The multi-channel fluorescence quantitative detection device according to claim 9, characterized in that: The optical inspection tank is also formed with a first heat preservation chamber and a second heat preservation chamber, and the plurality of inspection chambers are located between the first heat preservation chamber and the second heat preservation chamber; A heat preservation layer is arranged outside the optical inspection cell to keep the optical inspection cell warm.

12. A molecular diagnostic platform, characterized in that: A multi-channel fluorescence quantitative detection device comprising any one of claims 1 to 9.

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