A pipelined nucleic acid extraction and library preparation instrument

Abstract

A streamlined nucleic acid extraction and library preparation instrument includes a frame, a carriage device, a nucleic acid extraction device, a library construction device, a purification device mounted on the frame and located on one side of the carriage device, and a storage device mounted on the frame and located on the other side of the carriage device. The storage device is adapted to the nucleic acid extraction device, the library construction device, and the purification device through the carriage device, which moves the materials in the nucleic acid extraction device, the library construction device, the purification device, and the storage device. A pipetting device is installed above the frame, which is adapted to the nucleic acid extraction device and the storage device to move the liquid in the storage device to the nucleic acid extraction device.

Description

Technical Field

[0001] This invention belongs to the field of nucleic acid detection technology, specifically relating to a streamlined nucleic acid extraction and library preparation instrument. Background Technology

[0002] Nucleic acid amplification detection technology is widely used in various fields of life sciences such as molecular biology, medicine, and law. In particular, the development of high-throughput sequencing technology has played a significant role in both genetics and infection. However, the nucleic acid library preparation process used in high-throughput sequencing typically includes three steps: sample nucleic acid extraction, library construction, and library purification.

[0003] Currently available automated nucleic acid extraction and library preparation instruments have a low degree of automation, resulting in low quality of nucleic acids and libraries, which can easily lead to sequencing failure. The quality of nucleic acids and libraries directly affects the sequencing cost. Poor-quality libraries can indirectly increase sequencing steps and processing time, or even cause sequencing failure. Summary of the Invention

[0004] To address the above shortcomings, the technical problem to be solved by this invention is to provide a streamlined nucleic acid extraction and library preparation instrument that enables fully automated nucleic acid extraction, library construction and purification, and allows the final product to be directly applied to downstream nucleic acid detection.

[0005] To solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0006] A streamlined nucleic acid extraction and library preparation instrument includes a frame, a carriage device, a nucleic acid extraction device, a library construction device, a purification device mounted on the frame and located on one side of the carriage device, and a storage device mounted on the frame and located on the other side of the carriage device. The storage device is adapted to the nucleic acid extraction device, the library construction device, and the purification device through the carriage device, which moves the materials in the nucleic acid extraction device, the library construction device, the purification device, and the storage device. A pipetting device is installed above the frame, which is adapted to the nucleic acid extraction device and the storage device to move the liquid in the storage device to the nucleic acid extraction device.

[0007] As a preferred embodiment of the present invention, the vehicle traveling device includes a vehicle frame, a vehicle driving assembly, a rotating bracket, a transfer bracket, and a hooking assembly. The rotating bracket is slidably mounted on the vehicle frame via a slide rail. The vehicle driving assembly is connected to the rotating bracket and drives the rotating bracket to move. The transfer bracket and the hooking assembly are respectively mounted on the rotating bracket. The hooking assembly is adapted to the transfer bracket to transfer the test tube into the transfer bracket.

[0008] As a preferred embodiment of the present invention, the hook assembly includes a hook mounting plate, which slides within a rotating frame via a translation member. A lifting motor is mounted on the hook mounting plate, and a lifting gear is mounted on the lifting motor. A lifting rack is slidably mounted on the hook mounting plate, and a hook rod is fixedly mounted on the lifting rack. A hook is formed on the hook rod.

[0009] As a preferred embodiment of the present invention, the nucleic acid extraction device includes a oscillation mechanism and a sample addition mechanism that cooperate with each other; the oscillation mechanism includes an oscillation mounting plate, an oscillation base, an oscillation test tube rack, and an oscillation magnetic suction assembly. The oscillation base is mounted on the oscillation mounting plate via a shock-absorbing pad. The oscillation mounting plate is mounted on a frame. An oscillation motor is installed inside the oscillation base. An oscillation eccentric wheel is installed on the output shaft of the oscillation motor. The oscillation test tube rack is fixedly installed above the oscillation base. The oscillation magnetic suction assembly is mounted on the oscillation mounting plate.

[0010] As a preferred embodiment of the present invention, the sample feeding mechanism includes a sample feeding bracket, a sample feeding vertical component, a sample feeding longitudinal component, and a sample dispensing component. The sample feeding longitudinal component is mounted on the sample feeding bracket, the sample feeding vertical component is mounted on the sample feeding longitudinal component, and the sample dispensing component is mounted on the sample feeding vertical component and is adapted to the sample feeding vertical component.

[0011] As a preferred embodiment of the present invention, the cell culture preparation device includes a cell culture preparation test tube rack, a radiator, and a temperature control head. The temperature control head is installed on the radiator, and the radiator is installed on the cell culture preparation test tube rack. The cell culture preparation test tube rack and the temperature control head correspond one-to-one.

[0012] As a preferred embodiment of the present invention, the purification device includes a purification vibration mechanism and a purification sample loading mechanism, wherein the purification sample loading mechanism is adapted to the purification vibration mechanism; the purification vibration mechanism includes a purification base, a purification test tube rack and a purification motor, wherein the purification test tube rack is fixedly installed on the purification base and the purification motor is fixedly installed on the purification base, wherein an eccentric component is installed on the motor shaft of the purification motor, and the structure of the purification base and the purification test tube rack is consistent with the structure of the shaking base and the shaking test tube rack.

[0013] As a preferred embodiment of the present invention, the purification sample dispensing mechanism includes a horizontal moving component and a lifting component. The lifting component is mounted on the horizontal moving component and includes a lifting mounting base, a lifting screw, and a lifting slider. The lifting screw is mounted on the lifting mounting base, and the lifting slider is mounted on the lifting screw. A purification sample dispensing plate is mounted on the lifting slider. The purification sample dispensing plate is slidably connected to the lifting mounting base. A purification syringe, a purification needle, and a purification extruder are mounted on the purification sample dispensing plate. The purification extruder is adapted to the purification syringe.

[0014] As a preferred embodiment of the present invention, the purification extrusion part includes a purification connecting piece and an extrusion guide column. The purification connecting piece is installed on the extrusion guide column and is connected to the purification syringe. An extrusion spring is installed on the extrusion guide column for resetting the extrusion guide column. A pressing head is formed on the lifting mounting seat. The pressing head is adapted to the extrusion guide column and presses the extrusion guide column down by pressing the pressing head.

[0015] As a preferred embodiment of the present invention, the liquid transfer device includes a crossbeam, a transfer lifting component, an air pump, and a transfer mounting base. The transfer lifting component is mounted on the crossbeam, the transfer mounting base is mounted on the transfer lifting component, a transfer needle is mounted on the transfer mounting base, and the air pump is mounted on the transfer mounting base and is adapted to the transfer needle to drive the transfer needle to suck up or discharge material.

[0016] The beneficial effects of this invention are that the test tubes adapted to this device are transported between the nucleic acid extraction device, the library construction device, the purification device, and the storage device by the vehicle-mounted device, which improves the movement efficiency. In addition, the vehicle-mounted device can rotate relative to each other to achieve the spatial orientation of other modules, thereby improving the spatial layout of the nucleic acid extraction equipment and reducing the overall size of the nucleic acid extraction equipment. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the present invention.

[0018] Figure 2 This is a schematic diagram of the vehicle's movement mechanism.

[0019] Figure 3 yes Figure 2 A schematic diagram of the structure after being rotated at a certain angle.

[0020] Figure 4 This is a structural diagram of the collusion component.

[0021] Figure 5 This is a structural diagram of the test tube fitting.

[0022] Figure 6 This is a schematic diagram of the database construction device.

[0023] Figure 7 This is a schematic diagram of the purification vibration mechanism.

[0024] Figure 8 This is a schematic diagram of the purification and sample loading mechanism.

[0025] Figure 9 This is a magnified view of a portion of point A.

[0026] Figure 10 This is a schematic diagram of a pipetting device.

[0027] Figure 11This is a schematic diagram of the sample dispensing mechanism.

[0028] Figure 12 yes Figure 11 A schematic diagram of the structure after being rotated at a certain angle.

[0029] Figure 13 This is a schematic diagram of the oscillation mechanism.

[0030] Reference numerals: Frame 1, Traveling device 2, Traveling frame 2-1, Traveling drive assembly 2-2, Rotating bracket 2-3, Rotating base 2-3-1, Rotating frame 2-3-2, Rotating head 2-3-3, Rotating drive motor 2-3-4, Rotating gear 2-3-5, Rotating gear tooth 2-3-6, Rotating shaft 2-3-7, Bushing 2-3-8, Transfer bracket 2-4, Insertion slot 2-4-1, Hook assembly 2-5, Hook mounting plate 2-5-1, Lifting motor 2-5-2, Lifting gear 2-5-3, Lifting rack 2-5-4, Hook rod 2-5-5, Hook 2-5-6, Translation component 2-6;

[0031] Nucleic acid extraction device 3, oscillation mounting plate 3-1, oscillation base 3-2, oscillation tube rack 3-3, oscillation magnetic suction assembly 3-4, magnetic suction motor 3-4-1, magnetic suction rack 3-4-2, magnetic suction rod 3-4-3, magnet 3-4-4, shock absorption pad 3-5, oscillation motor 3-6, oscillation eccentric wheel 3-7, sample loading bracket 3-8, sample loading vertical assembly 3-9, sample loading horizontal plate 3-9-1, sample loading vertical lead screw 3-9-2, connector mounting plate 3 -9-3, pipette connector 3-9-4, extraction needle 3-9-5, sample plate 3-9-6, connector 3-9-7, spring 3-9-8, longitudinal sample loading assembly 3-10, longitudinal sample loading plate 3-10-1, longitudinal sample loading screw 3-10-2, longitudinal sample loading slider 3-10-3, sample dispensing assembly 3-11, sample dispensing top plate 3-11-1, sample dispensing seat 3-11-2, sample dispensing rack 3-11-3, sample dispensing motor 3-11-4;

[0032] Storage device 4, storage test tube rack 4-1, radiator 4-2, temperature controller 4-3;

[0033] Purification device 5, purification base 5-1, purification test tube rack 5-2, eccentric part 5-4, transverse moving assembly 5-5, lifting assembly 5-6, lifting mounting base 5-6-1, lifting screw 5-6-2, purification sample plate 5-6-3, purification syringe 5-6-4, purification needle 5-6-5, purification connecting piece 5-6-6, extrusion guide column 5-6-7, extrusion spring 5-6-8, pressing head 5-6-9;

[0034] Storage device 6, pipette tip holder 6-1, extraction reagent chamber 6-2, test tube holder 6-3, sample area 6-4, library preparation reagent chamber 6-5, temperature control area 6-6;

[0035] 7. Plugging device, 7-1. Crossbeam, 7-2. Plugging lifting component, 7-3. Air pump, 7-4. Plugging mounting base, 7-5. Plugging needle, 7-6. Slide rail, 7-7. Plugging drive component;

[0036] Test tube fittings 8, connecting plate 8-1, storage tube 8-2, purification tube 8-3, connector reserved tube 8-4, pipette tip 8-5, extraction reaction tube 8-6, hook groove 8-7. Detailed Implementation

[0037] The present invention will now be further described with reference to the accompanying drawings.

[0038] A streamlined nucleic acid extraction and library preparation instrument includes a frame 1, a carriage device 2, a nucleic acid extraction device 3, a library construction device 4, a purification device 5 mounted on the frame 1 and located on one side of the carriage device 2, and a storage device 6 mounted on the frame 1 and located on the other side of the carriage device 2. The storage device 6 is adapted to the nucleic acid extraction device 3, the library construction device 4, and the purification device 5 through the carriage device 2, and the carriage device 2 moves the materials in the nucleic acid extraction device 3, the library construction device 4, the purification device 5, and the storage device 6. A pipetting device 7 is installed above the frame 1, and the pipetting device 7 is adapted to the nucleic acid extraction device 3 and the storage device 6 to move the liquid in the storage device 6 into the nucleic acid extraction device 3.

[0039] The test tubes 8 adapted to this device are transported between the nucleic acid extraction device 3, the library construction device 4, the purification device 5, and the storage device 6 via the vehicle-mounted device 2, which improves the movement efficiency. Furthermore, the vehicle-mounted device can rotate relative to each other, enabling the other modules to be arranged in opposite directions in space, thereby improving the spatial layout of the nucleic acid extraction equipment and reducing the overall size of the nucleic acid extraction equipment.

[0040] The traveling device 2 includes a traveling frame 2-1, a traveling drive assembly 2-2, a rotating bracket 2-3, a transfer bracket 2-4, and a hooking assembly 2-5. The rotating bracket 2-3 is slidably mounted on the traveling frame 2-1 via a slide rail. The traveling drive assembly 2-2 is connected to the rotating bracket 2-3 and drives the rotating bracket 2-3 to move. The transfer bracket 2-4 and the hooking assembly 2-5 are respectively mounted on the rotating bracket 2-3. The hooking assembly 2-5 is adapted to the transfer bracket 2-4 to transfer the test tube 8 into the transfer bracket 2-4.

[0041] The transfer bracket 2-4 is used to place the test tube 8. In this embodiment, the vehicle device 2 is provided with two transfer brackets 2-4 and two hooking components 2-5, which can realize the one-in-one-out of the test tube 8, reduce the movement time, and increase the work efficiency.

[0042] In this embodiment, the vehicle drive assembly 2-2 adopts a motor and synchronous belt structure. The synchronous belt is connected to the rotating bracket 2-3. The synchronous belt is driven by the motor to move, thereby driving the rotating bracket 2-3 to move.

[0043] The rotating bracket 2-3 includes a rotating base 2-3-1, a rotating frame 2-3-2, and a rotating head 2-3-3. The rotating frame 2-3-2 is rotatably mounted on the rotating base 2-3-1 via the rotating head 2-3-3. The rotating base 2-3-1 is slidably mounted on the gantry frame 2-1 via a slide rail. A rotating drive motor 2-3-4 is also mounted on the rotating base 2-3-1. A rotating gear 2-3-5 is mounted on the rotating drive motor 2-3-4. A rotating gear 2-3-6 is connected to the outer side of the rotating head 2-3-3. The rotating gear 2-3-5 is connected to the rotating gear 2-3-6. The rotating frame 2-3-2 is driven to rotate relative to the rotating base 2-3-1 by the rotating drive motor 2-3-4. The relative rotation of the rotating frame 2-3-2 by the rotating drive motor 2-3-4 changes the orientation of the transfer bracket 2-4 and the hook assembly 2-5.

[0044] The rotating head 2-3-3 includes a rotating shaft 2-3-7 and a bushing 2-3-8 that are rotatably connected to each other. The rotating shaft 2-3-7 is fixedly connected to the rotating base 2-3-1. The bushing 2-3-8 is installed on the lower end of the rotating frame 2-3-2. The rotating gear teeth 2-3-6 are formed on the outside of the bushing 2-3-8 to facilitate relative rotation between the rotating base 2-3-1 and the rotating frame 2-3-2.

[0045] The hook assembly 2-5 includes a hook mounting plate 2-5-1, which is slidably mounted inside the rotating frame 2-3-2 via a translation component 2-6. A lifting motor 2-5-2 is mounted on the hook mounting plate 2-5-1, and a lifting gear 2-5-3 is mounted on the lifting motor 2-5-2. A lifting rack 2-5-4 is slidably mounted on the hook mounting plate 2-5-1, and a hook rod 2-5-5 is fixedly mounted on the lifting rack 2-5-4. A hook 2-5-6 is formed on the hook rod.

[0046] The lifting motor 2-5-2 drives the lifting rack 2-5-3 to move upward, the hook 2-5-6 hooks the test tube 8, and the translation component 2-6 drives the entire hook assembly 2-5 to move horizontally, thereby moving the test tube 8 into or out of the vehicle device.

[0047] The translation component 2-6 adopts a screw-slider structure, which is driven by a motor to push the hook mounting plate 2-5-1 horizontally.

[0048] The transfer bracket 2-4 is fixedly installed on the top of the rotating frame 2-3-2. The transfer bracket 2-4 has a groove 2-4-1 formed inside. The connecting plate 8-1 of the test tube 8 is installed inside the transfer bracket 2-4 through the groove 2-4-1.

[0049] The test tube assembly 8 includes a connecting plate 8-1, a storage tube 8-2, a purification tube 8-3, a connector pre-reserved tube 8-4, a pipette tip 8-5, and an extraction reaction tube 8-6. The storage tube 8-2, purification tube 8-3, connector pre-reserved tube 8-4, pipette tip 8-5, and extraction reaction tube 8-6 are respectively fixedly connected to the connecting plate 8-1. A hook part is fixedly connected to the end of the connecting plate 8-1, and a hook groove 8-7 is formed on the hook part. The hook 2-5-6 is detachably connected to the hook groove 8-7. The connecting plate 8-1 is used to be inserted into the test tube rack of each device.

[0050] The nucleic acid extraction device 3 includes a oscillation mechanism and a sample addition mechanism that work together. The oscillation mechanism vibrates the test tube 8, and the sample addition mechanism adds liquid into the test tube.

[0051] The oscillation mechanism includes an oscillation mounting plate 3-1, an oscillation base 3-2, an oscillation test tube rack 3-3, and an oscillation magnetic suction assembly 3-4. The oscillation base 3-2 is mounted on the oscillation mounting plate 3-1 via a shock-absorbing pad 3-5. The oscillation mounting plate 3-1 is mounted on the frame 1. An oscillation motor 3-6 is installed inside the oscillation base 3-2. An oscillation eccentric wheel 3-7 is mounted on the output shaft of the oscillation motor 3-6. The oscillation test tube rack 3-3 is fixedly mounted above the oscillation base 3-2. The oscillation magnetic suction assembly 3-4 is mounted on the oscillation mounting plate 3-1.

[0052] The oscillating motor 3-6 drives the oscillating eccentric wheel 3-7 to rotate, generating eccentric force during rotation. Due to the setting of the shock-absorbing pad 3-5, the oscillating base 3-2 can swing relative to the oscillating mounting plate 3-1, thereby realizing the oscillation of the test tube 8.

[0053] The oscillating magnetic suction assembly 3-4 includes a magnetic suction motor 3-4-1, a magnetic suction rack 3-4-2, and a magnetic suction rod 3-4-3. The magnetic suction motor 3-4-1 is fixedly mounted on the oscillating mounting plate 3-1. A gear is mounted on the motor shaft of the magnetic suction motor 3-4-1, and the gear is connected to the magnetic suction rack 3-4-2. The magnetic suction rod 3-4-3 is fixedly mounted on the magnetic suction rack 3-4-2, and a magnet 3-4-4 is mounted on the magnetic suction rod 3-4-3. The magnetic suction motor 3-4-1 drives the magnetic suction rack 3-4-2 to move up and down, which facilitates the magnetic suction operation of the test tube 8 as needed.

[0054] The sample feeding mechanism includes a sample feeding bracket 3-8, a sample feeding vertical component 3-9, a sample feeding longitudinal component 3-10, and a sample dispensing component 3-11. The sample feeding longitudinal component 3-10 is mounted on the sample feeding bracket 3-8, the sample feeding vertical component 3-9 is mounted on the sample feeding longitudinal component 3-10, and the sample dispensing component 3-11 is mounted on the sample feeding vertical component 3-9 and is compatible with the sample feeding vertical component 3-9.

[0055] The longitudinal loading assembly 3-10 includes a longitudinal loading plate 3-10-1, a longitudinal loading screw 3-10-2, and a longitudinal loading slider 3-10-3. The longitudinal loading plate 3-10-1 is mounted on the loading bracket 3-8, the longitudinal loading screw 3-10-2 is mounted on the loading bracket 3-8, and the longitudinal loading slider 3-10-3 is mounted on the longitudinal loading screw 3-10-2. The vertical loading assembly 3-9 is slidably mounted on the longitudinal loading plate 3-10-1 and is fixedly connected to the longitudinal loading slider 3-10-3. The longitudinal loading assembly 3-10 moves the pipette connector 3-9-4 longitudinally.

[0056] The vertical sample loading assembly 3-9 includes a horizontal sample loading plate 3-9-1, a vertical sample loading screw 3-9-2, and a vertical sample loading slider. The horizontal sample loading plate 3-9-1 is fixedly mounted on the vertical sample loading plate 3-10-1. The vertical sample loading screw 3-9-2 is mounted on the horizontal sample loading plate 3-9-1. The vertical sample loading slider is mounted on the vertical sample loading screw 3-9-2. A connector mounting plate 3-9-3 is mounted on the vertical sample loading slider. A pipette connector 3-9-4 and an extraction sampling needle 3-9-5 are installed inside the connector mounting plate 3-9-3. The vertical sample loading assembly 3-9 moves the pipette connector 3-9-4 and the extraction sampling needle 3-9-5 vertically.

[0057] The sample dispensing assembly 3-11 includes a sample dispensing top plate 3-11-1, a sample dispensing seat 3-11-2, and a sample dispensing rack 3-11-3. The sample dispensing rack 3-11-3 is fixedly mounted on the sample loading transverse plate 3-9-1. The sample dispensing seat 3-11-2 is slidably mounted on the sample loading transverse plate 3-9-1. A sample dispensing motor 3-11-4 is mounted on the sample dispensing seat 3-11-2. A gear is mounted on the motor shaft of the sample dispensing motor 3-11-4. The gear is connected to the sample dispensing rack 3-11-3, driving the sample dispensing seat 3-11-2 to move laterally. The sample dispensing top plate 3-11-1 is mounted on the sample dispensing seat 3-11-2. The sample dispensing top plate 3-11-1 is driven to move laterally by the sample dispensing motor 3-11-4, so that the sample dispensing top plate 3-11-1 corresponds to the pipette connectors 3-9-4 at different positions.

[0058] A sample loading plate 3-9-6 is slidably mounted on the connector mounting plate 3-9-3. A connector 3-9-7 is bent on the sample loading plate 3-9-6. The sample outlet plate 3-11-1 is adapted to the connector 3-9-7. A spring 3-9-8 is installed between the connector 3-9-7 and the connector mounting plate 3-9-3. The spring 3-9-8 facilitates the reset of the sample loading plate 3-9-6. During the sample loading process, the connector mounting plate 3-9-3 moves upward, and the connector 3-9-7 presses against the sample outlet plate 3-11-1. The connector mounting plate 3-9-3 continues to move upward, causing the sample loading plate 3-9-6 to move downward relative to the connector mounting plate 3-9-3, thereby squeezing out the liquid in the pipette connector 3-9-4.

[0059] The cell culture preparation device 4 includes a cell culture preparation test tube rack 4-1, a radiator 4-2, and a temperature control head 4-3. The temperature control head 4-3 is installed on the radiator 4-2, and the radiator 4-2 is installed on the cell culture preparation test tube rack. The cell culture preparation test tube rack 4-1 and the temperature control head 4-3 correspond one-to-one, so that the multi-station and multi-sample cell culture preparation process does not affect each other and the temperature is precisely controlled. The structure of the shaking test tube rack 3-3 and the cell culture preparation test tube rack 4-1 is consistent.

[0060] The purification device 5 includes a purification vibration mechanism and a purification sample dispensing mechanism, and the purification sample dispensing mechanism is adapted to the purification vibration mechanism.

[0061] The purification vibration mechanism includes a purification base 5-1, a purification test tube rack 5-2, and a purification motor. The purification test tube rack 5-2 is fixedly installed on the purification base 5-1, and the purification motor is fixedly installed on the purification base 5-1. An eccentric part 5-4 is installed on the motor shaft of the purification motor. The structure of the purification base 5-1 and the purification test tube rack 5-2 is the same as the structure of the vibration base 3-2 and the vibration test tube rack 3-3.

[0062] The purification sample dispensing mechanism includes a horizontal moving component 5-5 and a lifting component 5-6. The lifting component 5-6 is mounted on the horizontal moving component 5-5. The lifting component 5-6 includes a lifting mounting base 5-6-1, a lifting screw 5-6-2, and a lifting slider. The lifting screw 5-6-2 is mounted on the lifting mounting base 5-6-1, and the lifting slider is mounted on the lifting screw 5-6-2. A purification sample dispensing plate 5-6-3 is mounted on the lifting slider. The purification sample dispensing plate 5-6-3 is slidably connected to the lifting mounting base 5-6-1. A purification syringe 5-6-4, a purification needle 5-6-5, and a purification extruder are mounted on the purification sample dispensing plate 5-6-3. The purification extruder is adapted to the purification syringe 5-6-4.

[0063] The purification extrusion part includes a purification connecting piece 5-6-6 and an extrusion guide post 5-6-7. The purification connecting piece 5-6-6 is mounted on the extrusion guide post 5-6-7 and is connected to the purification syringe 5-6-4. An extrusion spring 5-6-8 is installed on the extrusion guide post 5-6-7 for resetting the extrusion guide post 5-6-7. A pressing head 5-6-9 is formed on the lifting mounting seat 5-6-1. The pressing head 5-6-9 is adapted to the extrusion guide post 5-6-7 and presses the extrusion guide post 5-6-7 down through the pressing head 5-6-9.

[0064] The storage device 6 includes a pipette tip holder 6-1 for storing pipette connectors 3-9-4, an extraction reagent chamber 6-2 for storing extraction reagents, a test tube holder 6-3 for placing test tubes 8 required for testing, a sample area 6-4 for storing samples, a library preparation reagent chamber 6-5, and a temperature control area 6-6.

[0065] The pipetting device 7 includes a crossbeam 7-1, a transfer lifting component 7-2, an air pump 7-3, and a transfer mounting base 7-4. The transfer lifting component 7-2 is mounted on the crossbeam 7-1, and the transfer mounting base 7-4 is mounted on the transfer lifting component 7-2. A transfer needle 7-5 is mounted on the transfer mounting base 7-4. The air pump 7-3 is mounted on the transfer mounting base 7-4 and is adapted to the transfer needle 7-5 to drive the transfer needle 7-5 to draw or dispense material. The pipetting device 7 moves liquids such as extraction reagents and samples to the corresponding workstations.

[0066] The material transfer lifting component 7-2 adopts a screw and slider mechanism, and the material transfer lifting component 7-2 is slidably mounted on the crossbeam 7-1 via a motor and a synchronous belt.

[0067] A slide rail 7-6 and a transfer drive 7-7 are installed above the frame 1. The pipetting device 7 is mounted on the frame 1 via the slide rail 7-6. The transfer drive 7-7 is a structure consisting of a motor and a synchronous belt. The motor drives the synchronous belt to move, thereby moving the pipetting device 7.

[0068] In this embodiment, two pipetting devices 7 are provided on the rack 1. One pipetting device 7 is used to pipette reagents for library construction, and the other pipetting device 7 is used to pipette reagents for nucleic acid extraction.

[0069] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention; therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

[0070] Although this document makes extensive use of terms corresponding to the figure labels, the possibility of using other terms is not excluded; these terms are used merely to more conveniently describe and explain the essence of the invention; interpreting them as any kind of additional limitation would be contrary to the spirit of the invention.

Claims

1. A streamlined nucleic acid extraction and library preparation instrument, characterized in that, The device includes a frame (1), a traveling device (2), a nucleic acid extraction device (3), a library building device (4), a purification device (5) mounted on the frame (1) and located on one side of the traveling device (2), and a storage device (6) mounted on the frame (1) and located on the other side of the traveling device (2). The storage device (6) is adapted to the nucleic acid extraction device (3), the library building device (4), and the purification device (5) through the traveling device (2), and the materials in the nucleic acid extraction device (3), the library building device (4), the purification device (5), and the storage device (6) are moved through the traveling device (2). A pipetting device (7) is installed above the frame (1), and the pipetting device (7) is adapted to the nucleic acid extraction device (3) and the storage device (6), and moves the liquid in the storage device (6) into the nucleic acid extraction device (3). The vehicle traveling device (2) includes a vehicle frame (2-1), a vehicle driving assembly (2-2), a rotating bracket (2-3), a transfer bracket (2-4), and a hooking assembly (2-5). The rotating bracket (2-3) is slidably mounted on the vehicle frame (2-1) via a slide rail. The vehicle driving assembly (2-2) is connected to the rotating bracket (2-3) and drives the rotating bracket (2-3) to move. The transfer bracket (2-4) and the hooking assembly (2-5) are respectively mounted on the rotating bracket (2-3). The hooking assembly (2-5) is adapted to the transfer bracket (2-4). The hook assembly (2-5) includes a hook mounting plate (2-5-1), which is slidably mounted in the rotating frame (2-3-2) via a translation component (2-6). A lifting motor (2-5-2) is mounted on the hook mounting plate (2-5-1), and a lifting gear (2-5-3) is mounted on the lifting motor (2-5-2). A lifting rack (2-5-4) is slidably mounted on the hook mounting plate (2-5-1), and a hook rod (2-5-5) is fixedly mounted on the lifting rack (2-5-4). A hook (2-5-6) is formed on the hook rod.

2. The automated nucleic acid extraction and library preparation instrument according to claim 1, characterized in that, The nucleic acid extraction device (3) includes a oscillation mechanism and a sample addition mechanism that cooperate with each other; the oscillation mechanism includes an oscillation mounting plate (3-1), an oscillation base (3-2), an oscillation test tube rack (3-3), and an oscillation magnetic suction assembly (3-4). The oscillation base (3-2) is mounted on the oscillation mounting plate (3-1) through a shock-absorbing pad (3-5). The oscillation mounting plate (3-1) is mounted on the frame (1). An oscillation motor (3-6) is installed inside the oscillation base (3-2). An oscillation eccentric wheel (3-7) is installed on the output shaft of the oscillation motor (3-6). The oscillation test tube rack (3-3) is fixedly installed above the oscillation base (3-2). The oscillation magnetic suction assembly (3-4) is installed on the oscillation mounting plate (3-1).

3. The automated nucleic acid extraction and library preparation instrument according to claim 1, characterized in that, The cell culture preparation device (4) includes a cell culture preparation test tube rack (4-1), a radiator (4-2), and a temperature control head (4-3). The temperature control head (4-3) is installed on the radiator (4-2), and the radiator (4-2) is installed on the cell culture preparation test tube rack. The cell culture preparation test tube rack (4-1) and the temperature control head (4-3) correspond one-to-one.

4. The automated nucleic acid extraction and library preparation instrument according to claim 1, characterized in that, The purification device (5) includes a purification vibration mechanism and a purification sample addition mechanism. The purification sample addition mechanism is adapted to the purification vibration mechanism. The purification vibration mechanism includes a purification base (5-1), a purification test tube rack (5-2), and a purification motor. The purification test tube rack (5-2) is fixedly installed on the purification base (5-1), and the purification motor is fixedly installed on the purification base (5-1). An eccentric part (5-4) is installed on the motor shaft of the purification motor. The structure of the purification base (5-1) and the purification test tube rack (5-2) is consistent with the structure of the shaking base (3-2) and the shaking test tube rack (3-3).

5. The automated nucleic acid extraction and library preparation instrument according to claim 1, characterized in that, The pipetting device (7) includes a crossbeam (7-1), a material lifting component (7-2), an air pump (7-3), and a material mounting base (7-4). The material lifting component (7-2) is mounted on the crossbeam (7-1), and the material mounting base (7-4) is mounted on the material lifting component (7-2). A material transfer needle (7-5) is mounted on the material mounting base (7-4). The air pump (7-3) is mounted on the material mounting base (7-4) and is adapted to the material transfer needle (7-5) to drive the material transfer needle (7-5) to suck up or discharge material.

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