A nutrient solution supply device for cell culture

Abstract

The application discloses a kind of nutrient solution supply device for cell culture, including supply box and multiple culture bottles, the culture bottle combination forms cell culture box, panel and pressing plate are equipped in the inside of supply box, cell culture box lower end is inserted into supply box, the center of the inside bottom surface of culture bottle is vertically installed with injection liquid pipe, the inside of injection liquid pipe is equipped with sealing rod, the outside of injection liquid pipe is equipped with multiple liquid outlet holes, the place of panel is opposite injection liquid hole is equipped with through hole, the place of pressing plate is opposite injection liquid hole is equipped with liquid supply pipe, the outer wall of liquid supply pipe is equipped with multiple liquid supply ports, and sealing cavity is formed between pressing plate and supply box.The application is simple in structure, can supply nutrition to multiple culture bottles at a time, and ensure that the nutrient solution injected into each culture bottle is matched, greatly increase the efficiency, and the above supply process culture bottle is always in sealed state, avoid the entry of external dust, ensure its safety.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell culture, in particular to a nutrient solution supply device for cell culture. BACKGROUND

[0002] Cell culture refers to all in vitro culture, which means that tissues are taken from the living body of an animal, separated into single cells by mechanical or enzymatic digestion, incubated and cultured under specific in vivo conditions that simulate physiological environment in vivo, so that they can survive and grow. In order to grow cells, nutrient solution needs to be continuously supplied for cell culture.

[0003] The existing method of supplying nutrient solution for cell culture generally injects nutrient solution into individual culture bottles by using a pipette. During the operation, since individual injection is required, the operation process is long, and multiple culture bottles are always in an open state, which is easy to enter dust and other stains, affecting the quality of cell culture. SUMMARY

[0004] The technical problem to be solved by the present application is to provide a nutrient solution supply device for cell culture, which does not need to open the culture bottle in the liquid supply state and can complete the injection of multiple culture bottles at one time, greatly increasing the efficiency.

[0005] The present application is realized by the following technical scheme: a nutrient solution supply device for cell culture, comprising a supply box and a plurality of culture bottles, the culture bottles are combined to form a cell culture box, a panel and a pressing plate are arranged inside the supply box, the panel is arranged above the pressing plate, the lower end of the cell culture box is inserted into the supply box and in contact with the panel, the width and length of the cell culture box match the length and width of the inside of the supply box, a liquid injection pipe is vertically installed at the center of the inside bottom surface of each culture bottle, the upper end of the liquid injection pipe is sealed, a sealing rod is arranged inside the liquid injection pipe, a plurality of liquid outlet holes are arranged on the outer surface of the liquid injection pipe, the liquid outlet holes are sealed by the sealing rod, a liquid injection hole is arranged on the bottom surface of each culture bottle and communicates with the liquid injection pipe, a through hole is arranged on the panel opposite to the liquid injection hole, a liquid supply pipe is installed on the pressing plate opposite to the liquid injection hole, the upper end of the liquid supply pipe is sealed, a plurality of liquid supply openings are arranged on the outer wall surface of the liquid supply pipe, a liquid inlet hole is arranged on the bottom surface of the pressing plate opposite to the liquid supply pipe, and a sealed cavity is formed between the pressing plate and the supply box.

[0006] As a preferred technical scheme, a rubber layer is installed on the outer wall surface of the sealing rod, the rubber layer is in contact with the inner wall surface of the liquid injection pipe, a first spring is installed on the upper end of the sealing rod, and the other end of the first spring is installed on the top surface inside the liquid injection pipe.

[0007] As a preferred technical scheme, the side surface of the pressing plate is provided with a rubber frame, the outer side surface of the rubber frame is in contact with the inner wall surface of the supply box, the two sides of the pressing plate and the panel are provided with a plurality of sliding blocks, the sliding blocks on the pressing plate are arranged through the rubber frame, the inner wall surface of the supply box is provided with a sliding groove opposite to the sliding blocks, the sliding groove is long and short, the sliding blocks on the panel are slidingly arranged in the longer sliding groove, the sliding blocks on the pressing plate are slidingly arranged in the shorter sliding groove, the bottom surface of the sliding block is provided with a second spring, the other end of the second spring is arranged on the bottom surface of the sliding groove, and the cross section of the sliding block and the sliding groove is in a trapezoidal structure.

[0008] As a preferred technical scheme, the outer side surface of the supply box is provided with a liquid adding pipe communicated with the sealing cavity, and the other end of the liquid adding pipe is threadedly connected with a sealing cover.

[0009] As a preferred technical scheme, the upper end of the culture bottle is provided with a breathable cover.

[0010] As a preferred technical scheme, the culture bottle on one side is provided with a scale.

[0011] As a preferred technical scheme, the supply box is made of transparent medical plastic material.

[0012] The culture box can be inserted into the matching supply box, the liquid supply pipe can be inserted into the liquid injection pipe after being pressed, the sealing rod is pushed away from the inner side of the liquid outlet hole, the liquid supply port is opposite to the liquid outlet hole, the nutrient liquid in the sealing cavity can be discharged along the liquid supply pipe, the liquid injection pipe, the liquid supply port and the liquid outlet hole, the nutrient supply of a plurality of culture bottles can be realized at one time, the nutrient liquid injected into each culture bottle is matched, the efficiency is greatly improved, and the culture bottles are in a sealed state during the above supply process, so that the entry of external dust is avoided and the safety is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0013] In order to more clearly illustrate the technical schemes in the embodiments of the present application or the prior art, the drawings needed to be used in the following embodiment or prior art description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0014] Figure 1 It is a schematic diagram of the overall structure of the present application.

[0015] Figure 2 It is Figure 1 It is a local enlarged view of A in the figure.

[0016] Figure 3 It is a vertical sectional view of the supply box in the present application. DETAILED DESCRIPTION

[0017] Embodiments of the present application are described below in detail with reference to the accompanying drawings, wherein like or similar elements are denoted by the same or similar reference symbols throughout the drawings. The embodiments described below are exemplary only, and are not intended to limit the present application.

[0018] All features disclosed in this specification, and / or all steps of any methods disclosed, may be combined in any combination, provided the features and / or steps are not mutually exclusive.

[0019] Any feature disclosed in this specification, unless stated otherwise, can be replaced by alternative features or equivalents having the same or a similar effect. That is, unless stated otherwise, each feature disclosed is one example only of a number of equivalent or similar features.

[0020] As shown in Figure 1 , Figure 2 and Figure 3 , a nutrient solution supply device for cell culture of the present application comprises a supply box 2 and a plurality of culture bottles 1 combined to form a cell culture box, the supply box 2 is internally provided with a panel 3 and a pressing plate 4, the panel 3 is arranged above the pressing plate 4, the lower end of the cell culture box is inserted into the supply box 2 and in contact with the panel 3, the width and length of the cell culture box match the length and width of the inside of the supply box 2, a liquid injection pipe 6 is vertically installed at the center of the bottom surface of the inside of each culture bottle 1, the upper end of the liquid injection pipe 6 is sealed, a sealing rod 7 is arranged inside the liquid injection pipe 6, a plurality of liquid outlet holes 18 are arranged on the outer surface of the liquid injection pipe 6, the liquid outlet holes 18 are sealed by the sealing rod 7, a liquid injection hole is arranged on the bottom surface of each culture bottle 1 and in communication with the liquid injection pipe, a through hole 11 is arranged on the panel 3 opposite to the liquid injection hole, a liquid supply pipe 12 is installed on the pressing plate 4 opposite to the liquid injection hole, the upper end of the liquid supply pipe 12 is sealed, a plurality of liquid supply openings 13 are arranged on the outer wall surface of the liquid supply pipe 12, a liquid inlet hole is arranged on the bottom surface of the pressing plate 4 opposite to the liquid supply pipe, and a sealed cavity 5 is formed between the pressing plate 4 and the supply box 2.

[0021] In this embodiment, a rubber layer 19 is installed on the outer wall surface of the sealing rod 7, the rubber layer 19 is in contact with the inner wall surface of the liquid injection pipe 6, a first spring 8 is installed on the upper end of the sealing rod 7, and the other end of the first spring 8 is installed on the top surface inside the liquid injection pipe 6.

[0022] In this embodiment, the side surface of the pressing plate 4 is provided with a rubber frame, the outer side surface of the rubber frame is in contact with the inner wall surface of the supply box 2, the two sides of the pressing plate 4 and the face plate 3 are provided with a plurality of sliding blocks 14, the sliding blocks on the pressing plate 4 are arranged through the rubber frame, the inner wall surface of the supply box 2 is provided with a sliding groove 21 opposite to the sliding blocks, the sliding groove 21 is arranged to be longer on one side and shorter on the other side, the sliding blocks on the face plate 3 are slidingly arranged in the longer sliding groove, the sliding blocks on the pressing plate 4 are slidingly arranged in the shorter sliding groove, the bottom surface of the sliding block 14 is provided with a second spring 15, the other end of the second spring 15 is arranged on the bottom surface of the sliding groove 21, and the cross section of the sliding block 14 and the sliding groove 21 is arranged in a trapezoidal structure.

[0023] In this embodiment, the outer side surface of the supply box 2 is provided with a liquid adding pipe 16 communicating with the sealed cavity, the other end of the liquid adding pipe 16 is threadedly connected with a sealing cover 17, after the supply box is turned over, the liquid adding pipe can be arranged upward, so that the nutrient solution can be filled into the sealed cavity along the liquid adding pipe, and vice versa, after the liquid adding pipe is downward, the nutrient solution in the inside can be poured out.

[0024] In this embodiment, the upper end of the culture bottle 1 is provided with a breathable cover 9, when the liquid is injected, the air in the culture bottle can be discharged along the breathable cover, so that the injection of the liquid is not affected.

[0025] In this embodiment, the culture bottle 1 on one side is provided with a scale, and the amount of the nutrient solution injected can be controlled through the scale.

[0026] In this embodiment, the supply box 2 is made of transparent medical plastic material, so that the capacity of the nutrient solution in the inside can be seen through the supply box.

[0027] The culture box can be inserted into the matching supply box, so that the culture box and the supply box are combined, when the nutrient solution needs to be added, the culture box can be directly pressed downward, so that the culture box is perpendicular to the inner wall of the supply box, after the culture box is pressed, the liquid supply pipe can be inserted into the liquid injection pipe, and the sealing rod is pushed away from the inner side of the liquid outlet hole, so that the liquid supply opening is opposite to the liquid outlet hole, after the culture box is continuously pressed, the face plate is in contact with the pressing plate, so that the pressing plate can be driven to move downward synchronously, the nutrient solution in the sealed cavity can be squeezed out along the liquid supply pipe, the liquid injection pipe, the liquid supply opening and the liquid outlet hole through the pressing plate, the nutrient supply of a plurality of culture bottles can be realized at one time, and the nutrient solution injected into each culture bottle is matched, the efficiency is greatly improved, and the culture bottles are always in a sealed state during the above supply process, so that the entry of external dust is avoided, and the safety is ensured.

[0028] After the above is completed, the culture box can be released, at this time, the face plate and the pressing plate can be lifted through the rebound of the second spring, so that the culture box is returned to the original position, the liquid supply pipe inserted into the culture box can be pulled out from the culture box, and the sealing rod can be pushed downward through the rebound of the first spring, so that the sealing rod is moved to the inner side of the liquid outlet hole again, and the liquid outlet hole is sealed again.

[0029] The above merely describes the specific embodiments of the present application, but the protection scope of the present application is not limited thereto, any change or replacement without creative labor should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be limited by the protection scope defined in the claims.

Claims

1. A nutrient solution supply device for cell culture, characterized in that: The invention comprises a supply box (2) and a plurality of culture bottles (1), wherein the culture bottles (1) are combined to form a cell culture box, wherein a panel (3) and a pressure plate (4) are provided inside the supply box (2), wherein the panel (3) is provided above the pressure plate (4), wherein the lower end of the cell culture box is inserted into the supply box (2) and contacts the panel (3), wherein the width and length of the cell culture box match the length and width inside the supply box (2), wherein an injection tube (6) is vertically installed at the center of the bottom surface of each culture bottle (1), wherein the upper end of each injection tube (6) is sealed, wherein each injection tube (6) is provided with a sealing rod (7), wherein the ... ) are provided with a plurality of liquid outlet holes (18) on the outer surface of each culture bottle (1), and the liquid outlet holes (18) are sealed by a sealing rod (7). A liquid injection hole connected to the liquid injection pipe is provided on the bottom surface of each culture bottle (1). A through hole (11) is provided at a position opposite to the liquid injection hole on the panel (3). A liquid supply pipe (12) is installed at a position opposite to the liquid injection hole on the pressure plate (4). The upper end of the liquid supply pipe (12) is sealed. A plurality of liquid supply ports (13) are provided on the outer wall surface of the liquid supply pipe (12). A liquid inlet hole is provided on the bottom surface of the pressure plate (4) opposite to the liquid supply pipe. A sealed cavity (5) is formed between the pressure plate (4) and the supply box (2).

2. The nutrient solution supply device for cell culture according to claim 1, characterized in that: The outer wall surface of the sealing rod (7) is provided with a rubber layer (19), and the rubber layer (19) is in contact with the inner wall surface of the liquid injection tube (6). The upper end of the sealing rod (7) is provided with a first spring (8), and the other end of the first spring (8) is provided on the top surface inside the liquid injection tube (6).

3. The nutrient solution supply device for cell culture according to claim 1, characterized in that: A rubber frame is installed on the side of the pressure plate (4), and the outer side of the rubber frame contacts the inner wall of the supply box (2). A plurality of sliders (14) are installed on both sides of the pressure plate (4) and the panel (3). The sliders on the pressure plate (4) are all set through the rubber frame. The inner wall of the supply box (2) is provided with a slide groove (21) facing the slider. The slide grooves (21) are set in two long and two short configurations. The sliders on the panel (3) are all slidably installed in the longer slide groove, and the sliders on the pressure plate (4) are all slidably installed in the shorter slide groove. A second spring (15) is installed on the bottom surface of the slider (14), and the other end of the second spring (15) is installed on the bottom surface of the slide groove (21). The cross-sections of the slider (14) and the slide groove (21) are both set in a trapezoidal structure.

4. The nutrient solution supply device for cell culture according to claim 1, characterized in that: A liquid adding pipe (16) communicating with the sealing cavity is installed on the outer surface of the supply box (2), and a sealing cover (17) is threadedly connected to the other end of the liquid adding pipe (16).

5. The nutrient solution supply device for cell culture according to claim 1, characterized in that: The upper ends of the culture bottles (1) are all equipped with air-permeable covers (9).

6. The nutrient solution supply device for cell culture according to claim 1, characterized in that: A scale is provided on the culture bottle (1) on one side.

7. The nutrient solution supply device for cell culture according to claim 1, characterized in that: The supply box (2) is made of transparent medical plastic material.

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