Blood sample separation container, blood sample separation device and sample analyzer

Abstract

The present invention discloses a blood sample separation container, a blood sample separation device, and a sample analyzer. The blood sample separation container is used for centrifugation and separation of whole blood samples during rotation. The blood sample separation container is provided with a sample chamber, a separation chamber, and a sedimentation chamber. The outer wall of the blood sample separation container is provided with a sample port connected to the sample chamber. Part of the sample chamber is located above the separation chamber and sedimentation chamber, while another part of the sample chamber is located below the separation chamber and sedimentation chamber. The separation chamber gradually contracts from one end closest to the sample chamber to the end farther away from the sample chamber. The blood sample separation container proposed by the present invention has a higher purity of plasma samples separated.

Description

Technical Field

[0001] The present invention relates to the technical field of blood sample detection, and in particular to a blood sample separation container, a blood sample separation device and a sample analyzer. Background Art

[0002] Blood is one of the most commonly used samples in clinical testing. Through biochemical, immunological, and other testing methods, a variety of physiological and pathological information can be obtained from blood, providing a basis for clinical diagnosis and treatment. Currently, most blood tests require the separation of blood cell components from plasma components during the whole blood sample pretreatment stage, and then subsequent testing is performed on the enriched blood cells or plasma separately.

[0003] In related technologies, whole blood samples are collected using blood collection tubes and then placed in a centrifuge for centrifugal separation. Because the blood sample in the blood collection tube is centrifuged, the blood cell components and plasma components are vertically layered. The plasma layer, where the plasma components reside, has a lower liquid level. When the plasma sample is sampled using a pipette, the pipette easily mixes the blood cell components below the plasma layer, resulting in a decrease in the purity of the plasma sample. Summary of the Invention

[0004] The main purpose of the present invention is to provide a blood sample separation container, aiming to improve the sampling purity of plasma samples.

[0005] To achieve the above-mentioned object, the present invention provides a blood sample separation container for centrifugally separating a whole blood sample during rotation, characterized in that the blood sample separation container is provided with a sample chamber, a separation chamber, and a sedimentation chamber that are sequentially connected, and an outer wall of the blood sample separation container is provided with a sample port that is connected to the sample chamber;

[0006] Part of the cavity structure of the sample chamber is located above the separation chamber and the precipitation chamber, and another part of the cavity structure of the sample chamber is located below the separation chamber and the precipitation chamber;

[0007] The separation cavity gradually shrinks from an end close to the sample cavity to an end far away from the sample cavity.

[0008] In one embodiment of the present invention, the sample chamber has a contraction chamber section, and at least a portion of the chamber structure of the contraction chamber section is located below the separation chamber;

[0009] The sample port is located above the contraction cavity section, and the contraction cavity section gradually contracts from an end close to the sample port to an end away from the sample port.

[0010] In one embodiment of the present invention, the contraction cavity section has a first flow guide wall close to and below the separation cavity, and the separation cavity has a second flow guide wall connected to the first flow guide wall;

[0011] The first guide wall is arranged in an inclined surface or an arc surface; and / or the second guide wall is arranged in an inclined surface or an arc surface.

[0012] In one embodiment of the present invention, a communication channel is further provided in the blood sample separation container;

[0013] The communication channel is communicated with the top space of the precipitation chamber and the separation chamber.

[0014] In one embodiment of the present invention, along a direction perpendicular to the extending direction of the communicating channel, the cross-sectional area of ​​the communicating channel is smaller than the cross-sectional area of ​​the precipitation chamber.

[0015] In one embodiment of the present invention, the volume of the sample chamber is greater than that of the separation chamber, and the volume of the separation chamber is greater than that of the precipitation chamber.

[0016] In one embodiment of the present invention, the outer wall of the blood sample separation container is provided with at least two positioning grooves, and the positioning grooves are used to achieve clamping and fixing of the blood sample separation container;

[0017] And / or, the outer wall of the blood sample separation container is provided with a limiting protrusion, and the limiting protrusion is used to achieve installation and fixation of the blood sample separation container;

[0018] And / or, the blood sample separation container is provided with a first connecting piece, and the first connecting piece is used to achieve installation and fixation of the blood sample separation container.

[0019] To achieve the above objectives, the present invention further provides a blood sample separation device, comprising:

[0020] Mounting seat;

[0021] a driving mechanism, the driving mechanism being arranged on the mounting seat;

[0022] a turntable, the turntable being connected to the driving mechanism, the driving mechanism driving the turntable to rotate, the turntable being provided with at least one limiting hole; and

[0023] At least one of the above-mentioned blood sample separation containers has an outer wall provided with a limiting convex portion, and one of the blood sample separation containers is inserted into one of the limiting holes, and one of the limiting convex portions abuts against the periphery of one of the limiting holes for limiting position.

[0024] In one embodiment of the present invention, the turntable is provided with a plurality of positioning springs. When a blood sample separation container is inserted into a limiting hole, the outer wall of the blood sample separation container abuts against a positioning spring to limit the position.

[0025] And / or, each of the blood sample separation containers is provided with a first connecting piece, and the turntable is provided with a plurality of second connecting pieces. When a blood sample separation container is inserted into a limiting hole, the first connecting piece on the blood sample separation container is magnetically fixed to a second connecting piece.

[0026] In one embodiment of the present invention, the blood sample separation device further comprises a code disk, the driving mechanism comprises an output shaft connected to the rotating disk, the code disk is sleeved on the output shaft and is located between the rotating disk and the driving mechanism;

[0027] The code disc is provided with a plurality of notches, and the mounting base is provided with a first sensor for sensing the notches; and / or, a block is provided on the side of the code disc facing away from the turntable, and the mounting base is provided with a second sensor for sensing the block.

[0028] To achieve the above object, the present invention further provides a sample analyzer, which includes the above blood sample separation device.

[0029] The technical solution of the present invention is to set a sample chamber, a separation chamber, and a sedimentation chamber that are connected in sequence in a blood sample separation container, so that part of the sample chamber structure is located above the separation chamber and the sedimentation chamber, and the other part of the structure is located below the separation chamber and the sedimentation chamber. The sample chamber, the separation chamber, and the sedimentation chamber are arranged horizontally as a whole. At the same time, the separation chamber is set to gradually shrink from the end close to the sample chamber to the end away from the sample chamber. In this way, when a whole blood sample is added to the sample chamber through the sample port and the blood sample separation container is rotated, the whole blood sample in the sample chamber will enter the separation chamber along the inclined bottom wall of the separation chamber under the action of the rotating centrifugal force, and then enter the sedimentation chamber. Because the density of the blood cell components in the whole blood sample is greater than the density of the plasma components, the centrifugal force exerted on the blood cell components is greater than the centrifugal force exerted on the plasma components. Therefore, after the blood cell components enter the sedimentation chamber, they will fill the internal space of the sedimentation chamber and squeeze out the plasma components, causing the plasma components to flow into the separation chamber, realizing the separation of the blood cell components and the plasma components. During the process of decelerating and stopping the blood sample separation vehicle, the separated plasma components flow back into the sample chamber along the inclined bottom wall of the separation chamber due to inertia, becoming the plasma sample; while the blood cell components remain in the sedimentation chamber, becoming the blood cell sample. At this point, the blood cell sample is contained in the sedimentation chamber, and the plasma sample is contained in the sample chamber. The blood cell sample and plasma sample are contained in separate chambers. When the plasma sample in the sample chamber is extracted through the sample port, the blood cell sample in the sedimentation chamber is not extracted, thereby improving the sampling purity of the plasma sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0031] Figure 1 This is a schematic structural diagram of the blood sample separation container of the present invention;

[0032] Figure 2 for Figure 1 A perspective structural diagram of a blood sample separation container in one embodiment;

[0033] Figure 3 for Figure 1 A perspective structural diagram of a blood sample separation container in another embodiment;

[0034] Figure 4 for Figure 1 A perspective structural diagram of a blood sample separation container in another embodiment;

[0035] Figure 5 Schematic diagram of the structure of the blood sample separation device of the present invention;

[0036] Figure 6 for Figure 5 A side structural diagram of the blood sample separation device;

[0037] Figure 7 for Figure 5 The coordination status of the middle positioning shrapnel and the blood sample separation container.

[0038] Description of Figure Numbers:

[0039] Label name Label name 1 Blood sample separation container 2 Mounting Block 1a Sample chamber 3 Drive mechanism 1a1 Contraction cavity section 31 output shaft 1a2 First guide wall 4 turntable 1b Separation chamber 4a Limiting hole 1b1 Second guide wall 41 Positioning shrapnel 1c Sedimentation chamber 5 Code disk 1d Sample delivery 5a gap 1e Connecting channel 51 stopper 1f Positioning slot 6 First sensor 11 Limiting convex part 7 Second sensor 12 First connecting piece

[0040] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0041] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0042] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0043] In the present invention, unless otherwise specified or limited, the terms "connection" and "fixation" should be understood in a broad sense. For example, "fixation" can mean fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two elements or interaction between two elements, unless otherwise specified. Those skilled in the art will be able to understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0044] In addition, in the present invention, descriptions such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. The meanings of "and / or" and "and / or" appearing throughout the text are the same, both indicating that three parallel solutions are included. Taking "A and / or B" as an example, it includes Solution A, or Solution B, or a solution in which both A and B are satisfied. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0045] The present invention provides a blood sample separation container 1 for realizing centrifugal separation of plasma components and blood cell components in a whole blood sample.

[0046] In one embodiment of the present invention, Figure 1 and Figure 2 As shown, the blood sample separation container 1 is provided with a sample chamber 1a, a separation chamber 1b and a sedimentation chamber 1c, and the outer wall of the blood sample separation container 1 is provided with a sample port 1d connected to the sample chamber 1a; wherein, part of the cavity structure of the sample chamber 1a is located above the separation chamber 1b and the sedimentation chamber 1c, and another part of the cavity structure of the sample chamber 1a is located below the separation chamber 1b and the sedimentation chamber 1c; the separation chamber 1b gradually shrinks from one end close to the sample chamber 1a to the end away from the sample chamber 1a.

[0047] In this embodiment, the sample chamber 1a is used to add and accommodate a whole blood sample, the separation chamber 1b is used to separate blood cell components and plasma components in the whole blood sample, and the precipitation chamber 1c is used to accommodate and precipitate blood cell components.

[0048] The sample chamber 1a, the separation chamber 1b and the sedimentation chamber 1c are connected in sequence, and part of the cavity structure of the sample chamber 1a is located above the separation chamber 1b and the sedimentation chamber 1c, and another part of the cavity structure of the sample chamber 1a is located below the separation chamber 1b and the sedimentation chamber 1c, so that the sample chamber 1a, the separation chamber 1b and the sedimentation chamber 1c are not arranged vertically along the direction of gravity. For example, the sample chamber 1a, the separation chamber 1b and the sedimentation chamber 1c can be distributed in sequence in the horizontal direction, or the sample chamber 1a, the separation chamber 1b and the sedimentation chamber 1c are distributed as a whole in the horizontal direction, but the sample chamber 1a, the separation chamber 1b and the sedimentation chamber 1c are staggered up and down. The arrangement of the sample chamber 1a, the separation chamber 1b, and the sedimentation chamber 1c allows the whole blood sample in the sample chamber 1a to flow laterally into the separation chamber 1b and the sedimentation chamber 1c under the action of the centrifugal force of the rotation when the blood sample separation container 1 is rotated. When the blood cell components and plasma components in the whole blood sample are separated, the blood cell components and the plasma components are respectively accommodated in the sedimentation chamber 1c and the sample chamber 1a. The blood cell components and the plasma components are accommodated in different chambers in the horizontal direction, rather than being vertically layered as in the centrifugal scheme of blood collection tubes. In this way, when the plasma component is sampled through the sample chamber 1a, the blood cell components will not be mixed and aspirated.

[0049] The separation chamber 1b is configured to gradually contract from one end close to the sample chamber 1a to the end away from the sample chamber 1a, so that the bottom wall of the separation chamber 1b becomes an inclined slope, arc surface, or wavy surface. The highest point of the bottom wall of the separation chamber 1b is close to the sedimentation chamber 1c, and the lowest point of the bottom wall of the separation chamber 1b is close to the sample chamber 1a. The inclined bottom wall of the separation chamber 1b reduces the resistance of the whole blood sample in the sample chamber 1a to entering the separation chamber 1b during centrifugation. It also facilitates the reflux of the plasma sample obtained by separation in the separation chamber 1b into the sample chamber 1a, facilitating the extraction of the plasma sample in the sample chamber 1a through the sample port 1d. Specifically, when a whole blood sample is added to the sample chamber 1a through the sample port 1d and the blood sample separation container 1 is rotated, the whole blood sample in the sample chamber 1a will enter the separation chamber 1b along the inclined bottom wall of the separation chamber 1b under the action of the rotating centrifugal force, and then enter the sedimentation chamber 1c. Because the density of the blood cell components in the whole blood sample is greater than that of the plasma components, the centrifugal force exerted on the blood cell components is greater than that exerted on the plasma components. Therefore, after the blood cell components enter the sedimentation chamber 1c, they will fill the internal space of the sedimentation chamber 1c and displace the plasma components, causing the plasma components to flow into the separation chamber 1b, thereby separating the blood cell components from the plasma components. During the process of decelerating and stopping the rotation of the blood sample separation vehicle, the separated plasma components flow back into the sample chamber 1a along the inclined bottom wall of the separation chamber 1b under the action of inertia, becoming the plasma sample; the blood cell components remain in the sedimentation chamber 1c, becoming the blood cell sample. Because the blood cell sample is contained in the sedimentation chamber 1c and the plasma sample is contained in the sample chamber 1a, the blood cell sample and the plasma sample are contained in separate chambers. When the plasma sample in the sample chamber 1a is extracted through the sample port 1d, the blood cell sample in the sedimentation chamber 1c will not be extracted, thereby improving the sampling purity of the plasma sample.

[0050] In one embodiment of the present invention, Figure 2 and Figure 4 As shown, the above-mentioned sample chamber 1a has a contraction chamber section 1a1, and at least part of the chamber structure of the contraction chamber section 1a1 is located below the separation chamber 1b; the sample port 1d is located above the contraction chamber section 1a1, and the contraction chamber section 1a1 gradually contracts from one end close to the sample port 1d to the end away from the sample port 1d.

[0051] In this embodiment, the sample port 1d is located above the sample chamber 1a and is connected to the sample chamber 1a. The sample port 1d is used for allowing the adding needle to add the whole blood sample into the sample chamber 1a, and for allowing the sampling needle to extract the plasma sample separated in the sample chamber 1a.

[0052] Because the separated plasma sample is finally contained in the sample chamber 1a, the separation chamber 1b has a contraction chamber section 1a1, and at least part of the chamber structure of the contraction chamber section 1a1 is located below the separation chamber 1b, so that the bottom chamber structure of the sample chamber 1a can become a chamber with a gradually shrinking space, so that when the plasma sample is contained in the sample chamber 1a, the liquid level of the plasma sample can be increased. In this way, the plasma sample in the sample chamber 1a can be sampled more conveniently through the sample port, avoiding the problem that the sampling needle easily collides with the bottom wall of the sample chamber 1a during sampling, and reducing the sampling accuracy requirements of the sampling needle.

[0053] In one embodiment of the present invention, Figure 2 and Figure 4 As shown, the contraction cavity section 1a1 has a first flow guide wall 1a2 close to and below the separation cavity 1b, and the first flow guide wall 1a2 is arranged in an inclined or arcuate surface.

[0054] In this embodiment, by configuring the first guide wall 1a2 as a slope or curved surface, the highest point of the first guide wall 1a2 is close to the separation chamber 1b. The slope or curved surface design of the first guide wall 1a2, which is higher at one end and lower at the other, provides less resistance to the flow of the whole blood sample. Thus, during the separation process, the whole blood sample in the sample chamber 1a can flow smoothly along the first guide wall 1a2 into the separation chamber 1b under the action of centrifugal force, completing the subsequent separation process. Furthermore, at the end of the separation process, the blood sample separation container 1 slows down and stops rotating. The plasma sample separated in the separation chamber 1b can also flow smoothly back into the sample chamber 1a along the first guide wall 1a2 under the action of inertia, allowing the separated plasma sample to be enriched in the contraction cavity section 1a1 in the sample chamber 1a, which is beneficial for increasing the volume and purity of the plasma sample extracted during the sampling process.

[0055] In one embodiment of the present invention, Figure 2 and Figure 4 As shown, the separation chamber 1b has a second guide wall 1b1 connected to the first guide wall 1a2, and the second guide wall 1b1 is arranged in an inclined surface or an arc surface.

[0056] In this embodiment, by configuring the second guide wall 1b1 as an inclined or curved surface, with the highest point of the second guide wall 1b1 close to the sedimentation chamber 1c, the design of the second guide wall 1b1 with one end higher than the other creates less resistance to the flow of the blood sample. Thus, during the separation process, the blood cell components within the separation chamber 1b can flow smoothly along the second guide wall 1b1 into the sedimentation chamber 1c under the action of centrifugal force, thereby achieving separation of the blood cell components from the plasma components. At the end of the separation process, the plasma components separated within the separation chamber 1b can flow smoothly back along the second guide wall 1b1 into the sample chamber 1a, achieving the discharge of the plasma sample.

[0057] One end of the second guide wall 1b1 extends toward the sedimentation chamber 1c, and the other end of the second guide wall 1b1 extends toward the first guide wall 1a2 and is connected to the first guide wall 1a2. The second guide wall 1b1 is connected to the first guide wall 1a2 to form a guide surface. The guide surface can allow the whole blood sample in the sample chamber 1a to smoothly enter the separation chamber 1b during the separation procedure, and can also allow the plasma sample separated in the separation chamber 1b to smoothly flow back to the sample chamber 1a at the end of the separation procedure. This reduces the flow resistance of the blood sample between the sample chamber 1a and the separation chamber 1b, and improves the efficiency of blood sample separation and sampling.

[0058] In one embodiment of the present invention, Figure 2 and Figure 4 As shown, the blood sample separation container 1 is further provided with a communication channel 1e; the communication channel 1e is communicated with the top space of the sedimentation chamber 1c and the separation chamber 1b.

[0059] In this embodiment, when the blood sample separation container 1 rotates, the whole blood sample in the separation chamber 1b is subjected to the dual effects of centrifugal force and gravity. Among them, the centrifugal force experienced by the blood cell components and plasma components in the whole blood sample is much greater than their respective gravitational forces. Moreover, the density of the blood cell components is greater than that of the plasma components, and the centrifugal force experienced by the blood cell components is greater than that experienced by the plasma components. Therefore, the blood cell components, which are more strongly affected by the centrifugal force, will more easily enter the sedimentation chamber 1c through the second guide wall 1b1 and enter the sedimentation chamber 1c through the connecting channel 1e. At the same time, the plasma components that may be present in the sedimentation chamber 1c are expelled into the separation chamber 1b through the connecting channel 1e, thereby achieving the separation of the blood cell components and plasma components in the whole blood sample and improving the purity of the plasma sample separated in the separation chamber 1b. In addition, the arrangement of the connecting channel 1e near the top of the sedimentation chamber 1c can effectively prevent the blood cell components separated in the sedimentation chamber 1c from flowing back into the separation chamber 1b through the connecting channel 1e when the blood cell components are settled and mixing with the separated plasma components, resulting in a decrease in the purity of the finally separated plasma sample.

[0060] In one embodiment of the present invention, Figure 2 and Figure 4 As shown, along a direction perpendicular to the extending direction of the communicating channel 1e, the cross-sectional area of ​​the communicating channel 1e is smaller than the cross-sectional area of ​​the precipitation chamber 1c.

[0061] In this embodiment, by designing the cross-sectional area of ​​the connecting channel 1e to be smaller than the cross-sectional area of ​​the sedimentation chamber 1c, the connecting channel 1e is made into a narrow channel structure. This can prevent the blood cell components separated in the sedimentation chamber 1c from flowing back through the connecting channel 1e to the separation chamber 1b and the sample chamber 1a when the blood sample separation container 1 slows down after the separation procedure, and mixing with the separated plasma components, thereby reducing the purity of the plasma sample.

[0062] In one embodiment of the present invention, Figure 2 and Figure 4 As shown, the volume of the sample chamber 1a is greater than that of the separation chamber 1b, and the volume of the separation chamber 1b is greater than that of the precipitation chamber 1c.

[0063] In this embodiment, the volume of the sample chamber 1a is larger than that of the separation chamber 1b, and the volume of the separation chamber 1b is larger than that of the sedimentation chamber 1c. Thus, the sample chamber 1a can accommodate a larger volume of the initially added whole blood sample, the separation chamber 1b can accommodate the plasma component of the whole blood sample, which has a higher content than the blood cell component, and the sedimentation chamber 1c can accommodate the blood cell component of the whole blood sample, which has a lower content. By setting the volumes of the sample chamber 1a, the separation chamber 1b, and the sedimentation chamber 1c to decrease in a stepwise manner, the sample chamber 1a, the separation chamber 1b, and the sedimentation chamber 1c can be ensured to have more reasonable volume sizes, thereby minimizing the volumes of the sample chamber 1a, the separation chamber 1b, and the sedimentation chamber 1c, and thus reducing the volume and material cost of the blood sample separation container 1. The volumes of the sample chamber 1a, the separation chamber 1b, and the sedimentation chamber 1c can be designed according to actual needs. For example, the volume ratio of the sample chamber 1a, the separation chamber 1b, and the sedimentation chamber 1c can be designed to be 5:4:3 to meet the requirements of blood sample separation and storage under normal circumstances. When the volume of the whole blood sample added to the sample chamber 1a is greater than the volume of the separation chamber 1b, the whole blood sample in the sample chamber 1a will have difficulty fully entering the separation chamber 1b and the sedimentation chamber 1c during the separation process, which can easily lead to inadequate separation of the whole blood sample. When the liquid level of the whole blood sample added to the sample chamber 1a is higher than the height of the connection point between the separation chamber 1b and the sedimentation chamber 1c, the whole blood sample in the sample chamber 1a will easily pass through the separation chamber 1b and enter the sedimentation chamber 1c, where it will be retained and become a dead sample, which will also make it difficult to fully separate the whole blood sample. To avoid this situation, the amount of whole blood sample added can be controlled so that the volume of the whole blood sample added to the sample chamber 1a is no greater than the volume of the separation chamber 1b, while also ensuring that the liquid level of the whole blood sample in the sample chamber 1a is no higher than the connection point between the separation chamber 1b and the sedimentation chamber 1c.

[0064] In one embodiment of the present invention, Figure 2 and Figure 4 As shown, the outer wall of the above-mentioned blood sample separation container 1 is provided with at least two positioning grooves 1f, and the positioning grooves 1f are used to achieve clamping and fixing of the blood sample separation container 1.

[0065] In this embodiment, the positioning groove 1f cooperates with the gripping mechanism for gripping the blood sample separation container 1, and the blood sample separation container 1 is clamped, fixed, and moved by the gripping mechanism. The blood sample separation container 1 is provided with a corresponding number of positioning grooves 1f according to the number of claws on the gripping mechanism, so that each claw on the gripping mechanism can engage with a positioning groove 1f on the blood sample separation container 1, thereby achieving reliable gripping of the blood sample separation container 1. Specifically, when the number of positioning grooves 1f is two, the two positioning grooves 1f can be provided on two opposite outer side walls of the blood sample separation container 1; when the number of positioning grooves 1f is multiple, the multiple positioning grooves 1f are provided on the outer peripheral wall of the blood sample separation container 1 along the circumference of the blood sample separation container 1.

[0066] The present invention also provides a blood sample separation device for realizing automatic centrifugal separation of plasma components and blood cell components in a whole blood sample.

[0067] In one embodiment of the present invention, see Figure 5 、 Figure 6 Combined with Figure 2 As shown, the above-mentioned blood sample separation device includes a mounting base 2, a driving mechanism 3, a turntable 4 and at least one blood sample separation container 1 mentioned above. The driving mechanism 3 is arranged on the mounting base 2; the turntable 4 is connected to the driving mechanism 3, and the driving mechanism 3 drives the turntable 4 to rotate. The turntable 4 is provided with at least one limiting hole 4a; the outer wall of the blood sample separation container 1 is provided with a limiting protrusion 11, and a blood sample separation container 1 is inserted into a limiting hole 4a, and a limiting protrusion 11 abuts against the periphery of a limiting hole 4a to limit the position.

[0068] In this embodiment, the mounting base 2 is used to mount and secure a drive mechanism 3, which is used to drive a turntable 4 to rotate horizontally. The turntable 4 is used to carry and position the blood sample separation container 1. The drive mechanism 3 can be fixed to the mounting base 2 by screwing, welding, or other means. The drive mechanism 3 can be a motor, etc. The drive mechanism 3 has an output shaft 31, which is rotatably disposed through the mounting base 2 and connected to the center of the turntable 4 to drive the turntable 4 to rotate smoothly. The turntable 4 is a disc structure having at least one limiting hole 4a, which is used to limit the position of the blood sample separation container 1. When the number of limiting holes 4a is greater than or equal to two, each limiting hole 4a is spaced apart. When there are multiple limiting holes 4a, the multiple limiting holes 4a can be spaced apart along the edge of the turntable 4 to fully utilize the space on the turntable 4, thereby increasing the centrifugal force applied to the blood sample in the blood sample separation container 1 within each limiting hole 4a, improving the efficiency of the blood sample separation process, and shortening the time required to complete the blood sample separation. When the blood sample separation container 1 is limited in the limiting hole 4a, the limiting protrusion 11 on the blood sample separation container 1 abuts against the upper surface of the turntable 4, preventing the blood sample separation container 1 from sliding down and out of the limiting hole 4a under the action of its own weight, thereby ensuring that the blood sample separation container 1 is reliably fixed on the turntable 4.

[0069] The specific structure of the blood sample separation container 1 in this embodiment refers to the above embodiments. Since this blood sample separation device adopts all the technical solutions of all the above embodiments, it has at least all the beneficial effects brought by the technical solutions of the above embodiments, which will not be described one by one here.

[0070] In one embodiment of the present invention, Figure 6 and Figure 7 As shown, the turntable 4 is provided with a plurality of positioning spring pieces 41 ; when a blood sample separation container 1 is inserted into a limiting hole 4 a , the outer wall of the blood sample separation container 1 abuts against a positioning spring piece 41 for limiting position.

[0071] In this embodiment, the positioning spring piece 41 can be arranged on the top surface or the bottom surface of the turntable 4, and each positioning spring piece 41 extends toward a limiting hole 4a. When a blood sample separation container 1 is inserted into a limiting hole 4a, a spring piece elastically abuts against the outer wall of the blood sample separation container 1 and pushes the blood sample separation container 1 toward the edge of the turntable 4. The positioning spring piece 41 cooperates with the limiting hole 4a to fix the blood sample separation container 1. In this way, when the turntable 4 rotates, the blood sample separation container 1 can be reliably fixed on the turntable 4, so that the blood sample separation container 1 will not shake or shift during the rotation of the turntable 4, which can avoid uncontrollable flow of blood samples in the separation container and ensure the efficiency and reliability of the blood sample separation procedure.

[0072] In one embodiment of the present invention, Figures 3 to 5 As shown, each blood sample separation container 1 is provided with a first connecting member 12, and the turntable 4 is provided with a plurality of second connecting members (not shown); when a blood sample separation container 1 is inserted into a limiting hole 4a, the first connecting member 12 on the blood sample separation container 1 is magnetically fixed to a second connecting member.

[0073] In this embodiment, the first connecting member 12 can be disposed on the bottom surface of the blood sample separation container 1. The bottom surface can be provided with a groove for accommodating and limiting the first connecting member 12, and the first connecting member 12 is fixed in the groove. The turntable 4 can be provided with a mounting plate adjacent to each limiting hole 4a, each mounting plate extending below a limiting hole 4a, and each mounting plate is provided with a second connecting member, which is provided corresponding to the first connecting member 12. When the blood sample separation container 1 is limited in the limiting hole 4a, the first connecting member 12 and the second connecting member are aligned and magnetically attracted to each other, so that the limiting protrusion 11 is tightly pressed against the periphery of the limiting hole 4a, and the blood sample separation container 1 is securely fixed to the turntable 4. This prevents uncontrolled flow of blood sample in the separation container when the blood sample separation container 1 rotates, thereby ensuring the efficiency and reliability of the blood sample separation process.

[0074] In one embodiment of the present invention, Figures 5 and 6As shown, the blood sample separation device also includes a code disk 5; the driving mechanism 3 has an output shaft 31 connected to the turntable 4, the code disk 5 is sleeved on the output shaft 31, and is located between the turntable 4 and the driving mechanism 3; the code disk 5 is provided with a plurality of notches 5a, and the mounting base 2 is provided with a first sensor 6, which is used to sense the notches 5a.

[0075] In this embodiment, the code disk 5 has a central hole, and the output shaft 31 of the drive mechanism 3 passes through the central hole of the code disk 5, so that the code disk 5 is sleeved and fixed to the output shaft 31 of the drive mechanism 3, and the code disk 5 and the turntable 4 maintain synchronous rotation under the drive of the drive mechanism 3. The first sensor 6 can be an infrared sensor or a photoelectric sensor. For example, taking the first sensor 6 as an infrared sensor, the infrared emitting end and the infrared output end of the first sensor 6 are respectively located on the upper and lower sides of the code disk 5. The code disk 5 has a state in which the main body is located between the infrared emitting end and the infrared output end, and blocks the infrared light emitted by the infrared emitting end. In this case, the first sensor 6 is in a signal interruption state. The code disk 5 also has a state in which the notch 5a is located between the infrared emitting end and the infrared output end. In this case, the infrared light emitted by the infrared emitting end can be received by the infrared receiving end, and the first sensor 6 is in a signal receiving state. When the code disk 5 rotates, the first sensor 6 switches between the above-mentioned signal interruption state and signal receiving state. By setting the distance between two adjacent notches 5a, the turntable 4 can rotate through the target angle when the first sensor 6 reaches the next signal receiving state from one signal receiving state. In this way, by setting the distance between the notches 5a, the first sensor 6 can control the drive mechanism 3 to drive the turntable 4 to always rotate the target angle between any two adjacent signal receiving states, so that different blood sample separation containers 1 are moved to the target position in sequence, and the blood sample separation container 1 is subjected to sample addition and sampling operations at the target position, thereby realizing the automation of the sample addition and sampling procedures of different blood sample separation containers 1.

[0076] In one embodiment of the present invention, Figures 5 and 6 As shown, a stopper 51 is provided on the side of the code disc 5 facing away from the turntable 4 , and a second sensor 7 is provided on the mounting base 2 . The second sensor 7 is used to sense the stopper 51 .

[0077] In this embodiment, the second sensor 7 can be an infrared sensor or a photoelectric sensor. When the block 51 moves to the vicinity of the second sensor 7 and blocks the second sensor 7, the code disk 5 and the turntable 4 are in their initial state. The second sensor 7 and the drive mechanism 3 are electrically connected to a control module such as a controller. The drive mechanism 3 drives the code disk 5 and the turntable 4 to rotate. When the block 51 does not block the second sensor 7, the second sensor 7 sends a first electrical signal to the control module. When the block 51 blocks the second sensor 7, the second sensor 7 sends a second electrical signal to the control module. When the control module receives the second electrical signal, it controls the motor braking, thereby resetting the code disk 5 and the turntable 4 to their initial state. This facilitates the subsequent automated sample addition and sampling operations of each blood sample separation container 1 using the blood sample separation device.

[0078] The present invention also provides a sample analyzer, which includes the above-mentioned blood sample separation device and is used to separate and detect whole blood samples.

[0079] In this embodiment, the sample analyzer includes a sample tray module, a reagent tray module, an automatic injection module, an incubation module, a sample reagent needle module, a magnetic separation module, and a combination of one or more components in a detection module, as well as the above-mentioned blood sample separation device. Among them, the sample tray module is used to provide samples; the reagent tray module is used to provide reagents; the automatic injection module is used to provide reaction cups; the incubation tray module is used to incubate the analyte (the analyte is a mixture of sample and reagent) to meet the conditions required for biochemical reactions and to perform transportation scheduling; the sample reagent needle module is used to transport centrifuged plasma or reagents; the magnetic separation module is used to wash and separate magnetic beads from unreacted waste liquid, obtain the washed magnetic beads and inject substrate luminescent liquid; and the detection module is used to obtain detection results for the analyte.

[0080] The specific structure of the blood sample separation device in this embodiment refers to the above embodiments. Since this sample analyzer adopts all the technical solutions of all the above embodiments, it has at least all the beneficial effects brought by the technical solutions of the above embodiments, which will not be described one by one here.

[0081] The above descriptions are merely optional embodiments of the present invention and do not limit the patent scope of the present invention. All equivalent structural transformations made using the contents of the present description and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included in the patent protection scope of the present invention.

Claims

1. A blood sample separation container for centrifugation of whole blood samples during rotation, characterized in that: The blood sample separation container is provided with a sample chamber, a separation chamber and a sedimentation chamber which are connected in sequence, and the outer wall of the blood sample separation container is provided with a sample port which is connected with the sample chamber; The upper cavity structure of the sample cavity is located above the separation cavity and the precipitation cavity, and the lower cavity structure of the sample cavity is located below the separation cavity and the precipitation cavity and is communicated with the separation cavity; The separation cavity gradually shrinks from an end close to the sample cavity to an end far away from the sample cavity; When the blood sample separation container rotates, at least a portion of the whole blood sample is located in the lower cavity structure and flows upward from the lower cavity structure into the separation cavity and the sedimentation cavity; The sample port is used for adding a whole blood sample to be separated into the sample cavity, and for extracting a separated plasma sample from the lower cavity structure.

2. The blood sample separation container according to claim 1, wherein The sample chamber has a contraction chamber section, and at least a portion of the chamber structure of the contraction chamber section is located below the separation chamber; The sample port is located above the contraction cavity section, and the contraction cavity section gradually contracts from an end close to the sample port to an end away from the sample port.

3. The blood sample separation container according to claim 2, wherein: The contraction cavity section has a first flow guide wall close to and below the separation cavity, and the separation cavity has a second flow guide wall connected to the first flow guide wall; The first guide wall is arranged in an inclined surface or an arc surface; and / or the second guide wall is arranged in an inclined surface or an arc surface.

4. The blood sample separation container according to any one of claims 1 to 3, wherein: The blood sample separation container is further provided with a communication channel; The communication channel is communicated with the top space of the precipitation chamber and the separation chamber.

5. The blood sample separation container according to claim 4, wherein: Along a direction perpendicular to an extending direction of the communication channel, a cross-sectional area of ​​the communication channel is smaller than a cross-sectional area of ​​the precipitation chamber.

6. The blood sample separation container according to any one of claims 1 to 3, characterized in that: The volume of the sample chamber is greater than that of the separation chamber, and the volume of the separation chamber is greater than that of the precipitation chamber.

7. The blood sample separation container according to any one of claims 1 to 3, characterized in that: The outer wall of the blood sample separation container is provided with at least two positioning grooves, and the positioning grooves are used to achieve clamping and fixing of the blood sample separation container; And / or, the outer wall of the blood sample separation container is provided with a limiting protrusion, and the limiting protrusion is used to achieve installation and fixation of the blood sample separation container; And / or, the blood sample separation container is provided with a first connecting piece, and the first connecting piece is used to achieve installation and fixation of the blood sample separation container.

8. A blood sample separation device, characterized in that: The blood sample separation device comprises: Mounting seat; a driving mechanism, the driving mechanism being arranged on the mounting seat; a turntable, the turntable being connected to the driving mechanism, the driving mechanism driving the turntable to rotate, the turntable being provided with at least one limiting hole; and At least one blood sample separation container according to any one of claims 1 to 7, wherein the outer wall of the blood sample separation container is provided with a limiting protrusion, and the blood sample separation container is inserted into the limiting hole, and the limiting protrusion abuts against the periphery of the limiting hole for limiting.

9. The blood sample separation device according to claim 8, characterized in that: The turntable is provided with a plurality of positioning springs. When a blood sample separation container is inserted into a limiting hole, the outer wall of the blood sample separation container abuts against a positioning spring to limit the position. And / or, each of the blood sample separation containers is provided with a first connecting piece, and the turntable is provided with a plurality of second connecting pieces. When a blood sample separation container is inserted into a limiting hole, the first connecting piece on the blood sample separation container is magnetically fixed to a second connecting piece.

10. The blood sample separation device according to claim 8 or 9, characterized in that: The blood sample separation device further includes a code disk, the driving mechanism includes an output shaft connected to the rotating disk, the code disk is sleeved on the output shaft and is located between the rotating disk and the driving mechanism; The code disc is provided with a plurality of notches, and the mounting base is provided with a first sensor for sensing the notches; and / or, a block is provided on the side of the code disc facing away from the turntable, and the mounting base is provided with a second sensor for sensing the block.

11. A sample analyzer, characterized in that: The sample analyzer includes the blood sample separation device according to any one of claims 8 to 10.

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