POCT blood cell analyzer and method of using the same

Abstract

The present application provides a POCT blood cell analyzer, which includes a detection seat and a pressure building component. The detection seat is used to install a test kit, and the test kit includes an impedance detection cell for impedance detection, and the impedance detection cell includes a front cell and a rear cell connected by micropores; the pressure building component is connected to the front cell and / or the rear cell gas path, and is used to provide pressure so that the test liquid in the front cell flows to the rear cell through the micropores, and is also used to provide pressure to the front cell or the rear cell before the test liquid is injected into the front cell so that the micropores are unobstructed.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a POCT blood cell analyzer, a pipette, a detection seat, a method for using the POCT blood cell analyzer, a test kit, an assembly seat, a sample detection device, and a microporous sheet. Background Art

[0002] Hematology analyzer, also known as blood cell analyzer, blood cell analyzer, blood cell counter, etc., is one of the most widely used instruments in hospital clinical testing. Most of the components in traditional hematology analyzers belong to the cleaning system, because before the next sample tube test, it is necessary to ensure that the traces of the previous sample tube are cleaned. The entire cleaning system is not only complex in structure and has many components, but also requires a large amount of reagents during the cleaning process, which takes a long time.

[0003] Compared with traditional blood cell analyzers, POCT blood cell analyzers have made great simplifications in instrument components. POCT blood cell analyzers can completely remove the cleaning fluid path related components in traditional blood analysis, greatly reducing the complexity and production cost of the instrument.

[0004] However, some existing POCT blood cell analyzers have overly simple functions, some have relatively complex structures, some have low automation levels, and some are relatively expensive. Summary of the invention

[0005] The present application provides a POCT blood cell analyzer, a pipette, a detection seat, a method for using the POCT blood cell analyzer, a test kit, an assembly seat, a sample detection device, and a microporous sheet to at least partially solve the above-mentioned technical problems.

[0006] In order to solve the above technical problems, a technical solution adopted in the present application is: to provide a POCT blood cell analyzer, the POCT blood cell analyzer includes a detection seat and a pressure building component, the detection seat is used to install a test kit, the test kit includes an impedance detection cell for impedance detection, the impedance detection cell includes a front cell and a rear cell connected by micropores; the pressure building component is connected to the front cell and / or the rear cell air path, and is used to provide pressure so that the test liquid in the front cell flows to the rear cell through the micropores, and is also used to provide pressure to the front cell or the rear cell before the test liquid is injected into the front cell so that the micropores are unobstructed.

[0007] The beneficial effects of the present application are as follows: different from the prior art, the present application provides a POCT blood cell analyzer, a pipette, a detection seat, a method for using the POCT blood cell analyzer, a test kit, an assembly seat, a sample detection device, and a microporous sheet, which are novel in structure, practical and reliable, low in cost, and can efficiently complete fully automated detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] In order to more clearly illustrate the technical solution in the present embodiment, the drawings required for use in the description of the embodiment are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work, among which:

[0009] Figure 1 It is a schematic diagram of the three-dimensional structure of a POCT blood cell analyzer provided in one embodiment of the present application;

[0010] Figure 2 This is a schematic diagram of the module structure of a POCT blood cell analyzer provided in one embodiment of the present application;

[0011] Figure 3 It is a schematic diagram of the internal structure of a POCT blood cell analyzer provided in one embodiment of the present application;

[0012] Figure 4 is a schematic diagram of the internal structure of a POCT blood cell analyzer provided in an embodiment of the present application, wherein the pipette is not shown;

[0013] Figure 5 It is a schematic diagram of the gas circuit structure of the pressure building system provided in one embodiment of the present application;

[0014] Figure 6 is a schematic diagram of the three-dimensional structure of a pipette provided in one embodiment of the present application;

[0015] Figure 7 It is a schematic diagram of the three-dimensional structure of a puncture head provided in one embodiment of the present application;

[0016] Figure 8 It is a bottom view structural schematic diagram of a metal shielding cover and a transmission mechanism of a POCT blood cell analyzer provided in one embodiment of the present application;

[0017] Fig. 9 It is a schematic diagram of the three-dimensional structure of a detection base of a POCT blood cell analyzer provided in one embodiment of the present application;

[0018] Fig.10 It is a schematic diagram of the explosion structure of the detection base of the POCT blood cell analyzer provided in one embodiment of the present application;

[0019] Fig.11 It is a side structural schematic diagram of the cooperation between the conductive support and the conductive column of the POCT blood cell analyzer provided by another embodiment of the present application;

[0020] Fig.12 1 is a schematic diagram of a top view of a kit provided in one embodiment of the present application;

[0021] Fig.13 It is a simplified three-dimensional perspective structural diagram of a POCT blood cell analyzer provided by another embodiment of the present application;

[0022] Fig.14 This is a flowchart of the use of a POCT blood cell analyzer provided in one embodiment of the present application;

[0023] Fig.15 It is a schematic diagram of an exploded structure of a kit provided in an embodiment of the present application from one perspective;

[0024] Fig.16 It is a schematic diagram of the exploded structure of the kit provided in one embodiment of the present application from another perspective;

[0025] Fig.17 It is a schematic cross-sectional structure diagram of a kit provided in one embodiment of the present application from one viewing angle;

[0026] Fig.18 It is a cross-sectional structural schematic diagram of a kit provided in one embodiment of the present application from another perspective;

[0027] Fig.19 It is a schematic diagram of the three-dimensional structure of a microporous sheet provided in one embodiment of the present application;

[0028] Fig. 20 yes Fig.19 Schematic diagram of the cross-sectional structure of the microporous sheet shown in FIG. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0030] It should be noted that if the embodiments of the present application involve directional indications (such as up, down, left, right, front, back...), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0031] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or suggesting their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0032] The present application provides a POCT blood cell analyzer and a method for using the same.

[0033] like Figures 1 to 14 As shown, the POCT blood cell analyzer includes a housing 10, a display screen 11, an outlet 12, a syringe 220, a frame 301, a pressure building system, a detection seat 300 and its transmission mechanism, a pipette 400 and its transmission mechanism, a metal shielding cover 500 and its transmission mechanism, etc.

[0034] The detection seat 300 can reciprocate in and out of the port 12 to facilitate the loading or unloading of the reagent kit 20. The pipette 400 and its transmission mechanism are used to realize the fully automated pre-processing of the sample to be tested (which can be pre-installed on the reagent kit 20). The fully automated pre-processing includes automatic sample addition, automatic reagent addition (the reagent kit 20 is pre-installed with diluent, hemolytic agent, staining and lysis reagent, etc.), automatic mixing (bubbling or stirring), etc. The pressure building system is used to provide the positive and negative pressure required for the fully automated pre-processing of the pipette 400. The metal shielding cover 500 and its transmission mechanism The structure is used to cooperate with the pressure building system for automatic detection. Automatic detection means that when the metal shielding cover 500 and the detection seat 300 are covered to form an electromagnetic shielding space, the air pressure connection device 510 of the pressure building system is connected to the pressure chamber 140 on the reagent box 20 (the pressure chamber 140 is connected to the rear tank of the impedance detection tank), and the pressure chamber 210 of the pressure building system begins to provide negative pressure or positive pressure so that the liquid in the front tank 120 of the impedance detection tank of the reagent box 20 flows through the micropores to the rear tank of the impedance detection tank of the reagent box 20 and records relevant parameters in the process. Among them, the air pressure connection device 510 of the pressure building system is connected to the metal shielding cover 500 and moves synchronously.

[0035] The pressure building system includes a pressure chamber 210, a first syringe 222 and a second syringe 223 which are linked, a driving member 224, an air filter 225, a first solenoid valve SV1, a second solenoid valve SV2, a third solenoid valve SV3, an air pressure connection device 510, etc.

[0036] The pipette 400 includes an air guide tube 401, an outer sleeve 402 sleeved on the outer periphery of the air guide tube 401, a synchronization block 403, a light shielding sheet 404, an optical coupler 405, a fixing plate 406, a through slot 407, a blocking member 4031 for unloading the mounting head (201 or 204), etc.

[0037] The metal shielding cover 500 is installed on the frame 301 through the mounting frame 520, and is lifted and lowered in the vertical direction through the motor 530 and the guide column 540. The metal shielding cover 500 is in the shape of a cover with an opening at the bottom, and the air pressure connection device 510 connected to the pressure building system extends into the interior of the metal shielding cover 500.

[0038] The detection seat 300 includes a main body 310, a conductive support 303 connected to the main body 310, a metal shielding seat (320, 321, 322) covering the main body 310, an optical detection component 330 embedded in the main body 310, a Peltier 340 arranged in a detection cavity close to the optical detection component 330, a heat sink 350 and a fan 360, etc.

[0039] The following will provide a detailed description based on various embodiments of the POCT blood cell analyzer.

[0040] The first embodiment, as Figures 1 to 13 As shown, this embodiment provides a POCT blood cell analyzer, which includes a housing 10 , a detection seat 300 , and a pipette 400 .

[0041] The detection seat 300 can be extended into or out of the housing 10 to receive the reagent kit 20 provided with an impedance detection cell. The detection seat 300 is provided with a power supply (eg, Fig. 9 The reagent kit 20 has a mounting head accommodating pool, which is used to install the mounting head (tip head 201 or puncture head 204).

[0042] The pipette 400 is disposed in the housing 10 and above the detection seat 300, and is used to perform corresponding operations on the reagent kit 20, including pipetting operations, bubble mixing operations, stirring mixing operations, and piercing the sealing film operations. When the mounting head needs to be unloaded (including normal detection completion and accidental power failure), the pipette 400 moves to a preset position outside the mounting head accommodating pool (for example, Fig.12 Performing the mounting head unloading operation in the front pool 120) can ensure that the pipette 400 will not repeatedly place the mounting head when the mounting head accommodating pool is already filled with a mounting head in the event of an unexpected power outage and restart. It can also shorten the running path when unloading the mounting head and reduce the alignment requirements when unloading the mounting head (generally, the diameter of the mounting head accommodating pool is relatively small, and precise alignment is required during unloading).

[0043] This embodiment also provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400 and a detector (which may be a detection element such as an optical coupler 405).

[0044] The test base 300 can be extended into or out of the housing 10 to receive the reagent box 20 provided with an impedance detection pool, and the test base 300 is provided with a power supply for impedance detection. The reagent box 20 has an installation head accommodating pool, and the installation head accommodating pool is used to install the installation head.

[0045] The pipette 400 is disposed in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent kit 20 .

[0046] The detector is used to detect whether the pipette 400 is loaded with a mounting head when the POCT blood cell analyzer needs to unload the mounting head. If it is detected that the pipette 400 is loaded with a mounting head, the pipette 400 moves to the mounting head accommodating pool or a preset position outside to perform the mounting head unloading operation. By setting up the detector, it is possible to know whether the pipette 400 is loaded with a mounting head and then selectively perform the unloading operation, which is relatively more intelligent.

[0047] This embodiment also provides a POCT blood cell analyzer, which is used to receive a test kit 20 and includes a detection seat 300 and a pipette 400.

[0048] The test base 300 is used to receive the reagent box 20 provided with an impedance detection pool, and the test base 300 is provided with a power supply for impedance detection. The reagent box 20 has a mounting head accommodating pool, and the mounting head accommodating pool is used to install the mounting head.

[0049] The pipette 400 is arranged above the detection seat 300, and is used to load the mounting head to perform corresponding operations on the reagent kit 20. When the mounting head needs to be unloaded, the pipette 400 moves to the mounting head accommodating pool or a preset position outside the mounting head accommodating pool to perform the mounting head unloading operation. In this embodiment, the POCT hematology analyzer also includes a detector (which can be a detection element such as an optical coupler 405), and the detector is used to detect whether the mounting head is loaded on the pipette 400 when the POCT hematology analyzer needs to unload the mounting head. If it is detected that the mounting head is loaded on the pipette 400, the pipette 400 unloads the mounting head to the mounting head accommodating pool or a preset position outside the mounting head accommodating pool. It is simpler to operate the pipette 400 to unload the mounting head to the preset position outside the mounting head accommodating pool, that is, directly find a large-caliber pool body nearby for unloading, which can avoid repeatedly placing the mounting head when the mounting head accommodating pool is already equipped with the mounting head, and can also shorten the running path when unloading the mounting head, while reducing the alignment requirements when unloading the mounting head.

[0050] The reagent box 20 has a plurality of cells, and the preset position is one of the cells. The plurality of cells include a mounting head accommodating pool and a plurality of functional pools that match the mounting head, and the pipette 400 unloads the mounting head into any functional pool. The functional pool may include a diluent pool 111, a front pool 120 (WBC detection pool or RBC detection pool), etc. The pipette 400 unloads the mounting head into any functional pool, which actually selects an absolute empty position and a pool position with a relatively large opening.

[0051] like Figure 6 As shown, the pipette 400 includes an air guide tube 401 and an outer sleeve 402. When the outer sleeve 402 moves axially relative to the air guide tube 401, the mounting head can be unloaded.

[0052] This embodiment also provides a method for using a POCT blood cell analyzer, the method comprising:

[0053] The pipette 400 of the POCT blood cell analyzer moves to the mounting head receiving tank to load the mounting head to perform the relevant operations of the first detection;

[0054] The first test has two situations: normal test completion and unexpected power failure. Before the second test, the mounting head used in the first test needs to be unloaded, and the pipette 400 of the POCT hematology analyzer moves to a preset position outside the mounting head storage tank to perform the mounting head unloading operation. This method of use can ensure that the pipette 400 will not repeatedly place the mounting head when the mounting head storage tank is already loaded with the mounting head, and can also shorten the running path when unloading the mounting head, while reducing the alignment requirements when unloading the mounting head.

[0055] This embodiment also provides a method for using a POCT blood cell analyzer, the method comprising:

[0056] The pipette 400 of the POCT blood cell analyzer moves to the mounting head receiving tank to load the mounting head to perform the relevant operations of the first detection;

[0057] The first test has two situations: normal test completion and unexpected power failure. Before the second test, the mounting head used in the first test needs to be unloaded. The detector can be used to detect whether the mounting head is loaded on the pipette 400 of the POCT blood cell analyzer.

[0058] If it is detected that a mounting head is loaded on the pipette 400 , the pipette 400 moves to a preset position outside the mounting head accommodation pool to perform a mounting head unloading operation.

[0059] This method of use can determine whether a mounting head is loaded on the pipette 400 and then selectively perform an unloading operation, which is relatively more intelligent.

[0060] This embodiment also provides a method for using a POCT blood cell analyzer, including:

[0061] The pipette of the POCT blood cell analyzer moves to the mounting head receiving pool to load the mounting head to perform related operations for the first detection;

[0062] The first test has two situations: normal test completion and unexpected power failure. Before the second test, the reagent kit 20 and the mounting head used in the first test need to be taken out, so it is necessary to check whether the POCT blood cell analyzer is equipped with the reagent kit 20;

[0063] Before the second test is to be performed, a detector may be used to detect whether the pipette 400 of the POCT blood cell analyzer is loaded with a mounting head;

[0064] If it is detected that the POCT blood cell analyzer is equipped with a reagent kit 20 and the pipette 400 is loaded with a mounting head, the pipette 400 is controlled to move to the mounting head accommodating pool or a preset position outside the mounting head accommodating pool to perform the mounting head unloading operation. When the mounting head unloading operation is performed at a preset position outside the mounting head accommodating pool, the mounting head will not be repeatedly placed when the mounting head accommodating pool is already loaded with a mounting head, and the running path when unloading the mounting head can also be shortened, while reducing the alignment requirements when unloading the mounting head.

[0065] The preset position may correspond to the inside of the detection seat 300 or to the outside of the detection seat 300. That is, the unloading operation is not limited to unloading the mounting head onto the detection seat 300 or onto the reagent kit 20, but may also be directly unloading to an area other than the detection seat 300 inside the instrument, for example, a mounting head recovery box is provided inside the instrument, and the maintenance personnel regularly empty the mounting head recovery box.

[0066] The outer sleeve 402 of the pipette 400 can unload the installation head by axially moving relative to the air guide tube 401 of the pipette 400. In a specific embodiment, the pipette 400 unloads the installation head through a fixed blocking member or a retractable blocking member 4031 that is movably arranged, and the blocking member 4031 is a retractable motor, a vertically or horizontally arranged electromagnet, or a U-shaped baffle, wherein the outer sleeve 402 can be connected to a synchronization block 403, and when the blocking member 4031 blocks the synchronization block 403 and the pipette 400 rises, the installation head is unloaded by the lower end of the outer sleeve 402.

[0067] Please refer to the second embodiment. Figures 1 to 13 , this embodiment provides a pipette 400, which includes an air guide tube 401 and a detection component.

[0068] The air guide tube 401 is used to load the mounting head (tip head 201 or puncture head 204 ), and the detection component is arranged on the side of the air guide tube 401 to detect whether the mounting head is loaded on the air guide tube 401 .

[0069] The pipette 400 also includes an outer sleeve 402, which is sleeved on the outer circumference of the air guide tube 401. When the air guide tube 401 is loaded with the mounting head, the air guide tube 401 is inserted into the mounting head so that the mounting head pushes the outer sleeve 402 to change its position along the axial direction of the air guide tube 401. The detection part is used to detect the position of the outer sleeve 402 and then determine whether the mounting head is sleeved on the air guide tube 401.

[0070] When the mounting head is sleeved on the air guide tube 401, the outer sleeve 402 is located at the first position of the air guide tube 401 ( Figure 6 In the state position shown in the figure), when the mounting head is not sleeved on the air guide tube 401, the outer sleeve 402 is located at the second position of the air guide tube 401, and the first position is higher than the second position.

[0071] A light blocking sheet 404 is connected to the outer sleeve 402, and the detection component is an optical coupler 405 that is matched with the light blocking sheet 404. When the optical channel of the optical coupler 405 is blocked by the light blocking sheet 404, it means that a mounting head is connected to the air guide tube 401. When the optical channel of the optical coupler 405 is not blocked by the light blocking sheet 404, it means that the air guide tube 401 is not connected to the mounting head.

[0072] The pipette 400 includes a fixing plate 406 disposed on the side of the air guide tube 401, and the optical coupler is disposed on the fixing plate 406. The air guide tube 401 of the pipette 400 can move up and down alone, and the pipette 400 as a whole can move in two dimensions or three dimensions.

[0073] The fixing plate 406 is provided with a through slot 407, a pair of optical transceiver parts of the optical coupler 405 extend into the through hole and are located on the side of the air duct 401, and a synchronization block 403 is also connected to the outer sleeve 402, and the synchronization block 403 extends into the through slot 407. When the air duct 401 rises and the synchronization block 403 is blocked, the mounting head is unloaded. After the mounting head is unloaded, the outer sleeve 402 is still sleeved on the outer periphery of the air duct 401, and because the synchronization block 403 extends into the through slot 407, the outer sleeve 402 will not fall off relative to the air duct 401, but is naturally supported at the bottom of the through slot 407 (that is, the second position).

[0074] This embodiment also provides a method for using a POCT blood cell analyzer, comprising the following steps:

[0075] Before performing the pipetting, it is determined whether the air guide tube 401 of the pipette 400 is equipped with a mounting head;

[0076] If it is detected that the air guide tube 401 of the pipette 400 is not equipped with a mounting head, an alarm is issued. Since the pipelines of the POCT blood cell analyzer are all airflows, not liquid flows, and liquids cannot be sucked in the pipelines, the air guide tube 401 must be connected with a mounting head (tip head 201) before corresponding operations can be performed, so it is necessary to detect whether the air guide tube 401 of the pipette 400 is equipped with a mounting head.

[0077] In the step of detecting whether the air tube 401 of the pipette 400 is equipped with a mounting head, an outer sleeve 402 is sleeved on the outer periphery of the air tube 401 , and the position of the outer sleeve 402 is detected by the detection member to determine whether the air tube 401 is sleeved with a mounting head.

[0078] When the mounting head is sleeved on the air guide tube 401, the outer sleeve 402 is located at the first position of the air guide tube 401, and when the mounting head is not sleeved on the air guide tube 401, the outer sleeve 402 is located at the second position of the air guide tube 401, and the first position is higher than the second position.

[0079] The detection part is an optical coupler 405, and the outer sleeve 402 is provided with a light blocking sheet 404 that matches the optical coupler 405. When the light blocking sheet 404 blocks the optical channel of the optical coupler 405, it is determined that the mounting head outer sleeve 402 is sleeved on the air guide tube 401.

[0080] This embodiment provides a POCT blood cell analyzer, including a housing 10, a detection seat 300, a pipette 400, and a detection element.

[0081] The detection seat 300 can be extended into or out of the shell 10, and is used to receive the reagent kit 20 provided with an impedance detection cell; the pipette 400 is arranged in the shell 10 and is located above the detection seat 300, and the pipette 400 includes an air guide tube 401, and the air guide tube 401 is used to load the installation head (tip head 201 or puncture head 204); the detection component is arranged on the side of the air guide tube 401, and is used to detect whether the installation head is loaded on the air guide tube 401. The detection component can be an optical coupler 405 or other detection element that can detect the presence or absence of an object.

[0082] In this embodiment, the detection element is provided to know the loading status of the mounting head, so as to prevent the pipette from performing a pipetting operation without loading the mounting head and contaminating the internal gas path of the POCT blood cell analyzer.

[0083] Please refer to the third embodiment. Figures 1 to 13The present embodiment provides a POCT blood cell analyzer, which includes a detection seat 300, a pipette 400 arranged above the detection seat 300, and a pressure building system. The detection seat 300 is used to receive a reagent kit 20 for loading. The reagent kit 20 is provided with a front pool 120 and a rear pool connected by micropores. The pressure building system includes a pressure chamber 210, an air pressure connection device 510, a first syringe 222, a second syringe 223, and a driving member 224.

[0084] The first syringe 222 is connected to the pressure chamber 210 or the pipette 400, and is used to establish positive pressure or negative pressure in the pressure chamber 210, and is also used to assist the pipette 400 in pipetting, or to bubble and mix the liquid in the front pool 120. The second syringe 223 is connected to the pipette 400, and is used to aspirate samples and reagents. The driving member 224 is used to drive the first syringe 222 and the second syringe 223 at the same time. The first syringe 222 and the second syringe 223 can be linked syringes to facilitate sharing the same driving member 224. The driving member 224 can be a motor. Of course, the first syringe 222 and the second syringe 223 can also be separate syringes and are independently controlled by different motors.

[0085] The POCT blood cell analyzer further includes solenoid valves, which include a first solenoid valve SV1, a second solenoid valve SV2, and a third solenoid valve SV3.

[0086] The first solenoid valve SV1 selectively connects the first syringe 222 with the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively connects the first solenoid valve SV1 with the pressure chamber 210 and the outside atmosphere, and the outside atmosphere is connected to the second solenoid valve SV2 through the air filter 225 and the pipeline T10; the third solenoid valve SV3 selectively connects the air pressure connecting device 510 with the pressure chamber 210, and when the third solenoid valve SV3 is energized, the pipelines T8 and T7 are connected through the third solenoid valve SV3.

[0087] The first syringe 222 can inhale air through the air filter 225, T10 pipeline, the second solenoid valve SV2, T5 pipeline, the first solenoid valve SV1, and T1 pipeline, and then establish positive pressure to the pressure chamber 210 through the T1 pipeline, the first solenoid valve SV1, T5 pipeline, the second solenoid valve SV2, and T6 pipeline.

[0088] The first syringe 222 can suck away the gas in the pressure chamber 210 through the T1 pipeline, the first solenoid valve SV1, the T5 pipeline, the second solenoid valve SV2, and the T6 pipeline to establish a negative pressure, and then exhaust the gas through the T1 pipeline, the first solenoid valve SV1, the T5 pipeline, the second solenoid valve SV2, the T10 pipeline, and the air filter 225.

[0089] After the pressure chamber 210 establishes positive pressure or negative pressure, it can output positive pressure or negative pressure through the T7 pipeline, the third solenoid valve SV3, the T8 pipeline, and the air pressure connection device 510. Among them, the T8 pipeline can be a relatively hard pipeline, that is, the hardness of the T8 pipeline is greater than that of the second conduit 221. The greater hardness of the T8 pipeline can prevent the pipe wall of the T8 pipeline from sticking together to block the internal air flow channel of the T8 pipeline when outputting negative pressure.

[0090] When the first solenoid valve SV1 connects the T1 pipeline with the T2 pipeline, the positive pressure or negative pressure generated by the first syringe 222 and the second syringe 223 acts on the pipette 400 at the same time, that is, when a large amount of sample is required to be sucked or pushed, the first syringe 222 can play a role in assisting the pipetting.

[0091] The volume of the first syringe 222 is greater than that of the second syringe 223. The first syringe 222 is mainly used for building pressure, and the second syringe 223 is mainly used for pushing / sucking samples. Of course, both syringes can be used simultaneously for building pressure or pushing / sucking samples as needed.

[0092] The volume ratio of the first syringe 222 to the volume ratio of the second syringe 223 can be 80 to 120:1. For example, the volume of the first syringe 222 is 10 ml, and the volume of the second syringe 223 is 100 μl. The driving member 224 drives the piston rods of the first syringe 222 and the second syringe 223 through the linkage plate 231 so that the first syringe 222 and the second syringe 223 move synchronously. The piston rod of the second syringe 223 is sealed with the tail end of the outer barrel of the second syringe 223.

[0093] The POCT blood cell analyzer also includes a housing 10 and a frame 301 . The pressure chamber 210 is disposed on the housing 10 or on the frame 301 . The pipette 400 , the first syringe 222 , the second syringe 223 , and the driving member are disposed on the frame 301 .

[0094] The detection seat 300 is slidably or rotatably disposed relative to the frame 301 and is located below the pipette 400 .

[0095] The POCT blood cell analyzer also includes a metal shielding cover 500. The detection seat 300 can move relative to the frame 301 to the liquid preparation position (i.e. Figure 3 The detection seat 300 can move relative to the rack 301 to the sample detection station (not shown in the figure, corresponding to the metal shielding cover 500 directly below), and the metal shielding cover 500 is used to drop at the sample detection station to cooperate with the detection seat 300 to form a relatively closed metal cavity to shield external electromagnetic interference. The rack 301 can form a double-layer structure, with the detection seat 300 arranged at the lower layer, and the pipette 400, the metal shielding cover 500 and the syringe 220 arranged at the upper layer.

[0096] like Figure 1 As shown, the housing 10 is provided with a display screen 11 and an outlet 12, and the detection seat 300 is used to move to the outlet 12 to receive the reagent kit 20 and load it into the detection seat 300, and the distance between the liquid preparation station and the outlet 12 is less than the distance between the sample detection station and the outlet 12, that is, the outlet 12 is sequentially the liquid preparation station and the sample detection station toward the inside of the instrument. The detection seat 300 reciprocates between the loading station, the liquid preparation station and the sample detection station, and the liquid preparation station is located between the loading station and the sample detection station. In other implementations, the liquid preparation station and the sample detection station can also be the same station.

[0097] This embodiment also provides a method for using the aforementioned POCT blood cell analyzer, comprising the following steps:

[0098] Controlling the movement of the driving member 224 to establish positive pressure or negative pressure in the pressure chamber 210 through the first syringe 222;

[0099] Controlling the movement of the driving member 224 to perform pipetting through the second syringe 223 and the pipette 400;

[0100] The pressure release of the pressure chamber 210 is controlled to apply positive pressure to the front pool 120 or negative pressure to the rear pool through the air pressure connection device 510 to enable the liquid in the front pool 120 to flow to the rear pool through the micropores.

[0101] The POCT blood cell analyzer provided in this embodiment has a reasonable layout, a compact structure, and is easy to use.

[0102] Please refer to the fourth embodiment Figures 1 to 13 This embodiment provides a test kit 20, which includes a box body 100 and a microfluidic detection chip 600.

[0103] The box body 100 includes an impedance detection cell (including a front cell 120, a rear cell, and a pressure action chamber 140 that are connected) for performing impedance detection.

[0104] The microfluidic detection chip 600 is integrally connected, assembled or not connected to the box body 100 and is located at the side of the impedance detection cell.

[0105] The microfluidic detection sheet 600 is provided with a sample addition hole 601 for receiving the sample and then spreading it in the microfluidic detection sheet 600 .

[0106] The box body 100 also includes a diluent pool 111, a hemolytic agent pool 106, an optical detection pool, and a staining and lysis pool 602. The diluent pool 111 is used to encapsulate the diluent, the hemolytic agent pool 106 is used to encapsulate the hemolytic agent, the staining and lysis pool 602 is used to encapsulate the staining and lysis reagent, and the optical detection pool is integrally connected or assembled with the box body 100.

[0107] The box body 100 also includes a mounting head accommodating pool and a sample accommodating pool. The mounting head accommodating pool is used to place the mounting head (tip head 201 or) puncture head 204, and the sample accommodating pool is used to place the sample tube.

[0108] Please also read Fig.13 This embodiment also provides a POCT blood cell analyzer, which includes a detection base 300, an image recognition base 610, and an image recognition device 620.

[0109] The detection base 300 is provided with a power supply (eg Fig. 9 The conductive support 303 shown in the figure), the detection seat 300 is used to receive the aforementioned reagent kit 20, the image recognition base 610 is arranged on the side of the detection seat 300, and the image recognition device 620 is arranged above the image recognition base 610, and is used to cooperate with the image recognition base 610 to perform image detection on the microfluidic detection sheet 600.

[0110] The POCT blood cell analyzer also includes a pipette 400, which is arranged above the detection seat 300. The POCT blood cell analyzer includes a metal shielding cover 500 matching the detection seat 300, and the metal shielding cover 500 can perform lifting or rotating motion. The image recognition device 620 is independently arranged or connected to the metal shielding cover 500.

[0111] The POCT blood cell analyzer and the reagent kit 20 provided in this embodiment can simultaneously support impedance detection and microfluidic image detection, optimize the joint detection efficiency, and achieve better results.

[0112] Please refer to the fifth embodiment Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a frame 301, a detection seat 300, and a pipette 400.

[0113] The detection seat 300 is slidably or rotatably arranged relative to the frame 301, and the detection seat 300 reciprocates between the loading station and the sample detection station. When the detection seat 300 slides out or rotates out of the frame 301, it is located at the loading station to receive the reagent kit 20 for loading or allow the reagent kit 20 to be taken out.

[0114] The pipette 400 is disposed in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent kit 20 .

[0115] The POCT blood cell analyzer also includes a liquid preparation station; the liquid preparation station is between the loading station and the sample detection station; or the liquid preparation station and the sample detection station are the same station.

[0116] The detection seat 300 is in a semi-enclosed shape with an upper opening. The detection seat 300 is snap-fitted with the reagent kit 20 . The detection seat 300 includes a main body 310 and a metal shielding seat ( 320 , 321 , 322 ) covering the outer periphery of the main body 310 .

[0117] The POCT blood cell analyzer also includes a metal shielding cover 500 matched with the metal shielding seat (320, 321, 322). The metal shielding cover 500 is arranged above the detection seat 300 and is configured to be able to move in the vertical direction at the sample detection station, and the vertical edge of the metal shielding cover 500 abuts against the upper surface of the metal shielding seat (320).

[0118] The POCT blood cell analyzer also includes a pressure building system, one end of which is provided with a pneumatic connection device 510 extending into the metal shielding cover 500 and moving synchronously with the metal shielding cover 500, and the reagent kit 20 is provided with a pressure application chamber 140 connected to the pneumatic connection device 510.

[0119] This embodiment provides a method for using a POCT blood cell analyzer, the method comprising the following steps:

[0120] The detection seat 300 slides out or rotates out of the frame 301 to receive the reagent kit 20 at the loading station;

[0121] The detection seat 300 slides into or rotates into the frame 301 and moves to the liquid preparation station;

[0122] The pipette 400 moves to load the pre-placed mounting head (tip head 201 or puncture head 204) on the reagent kit 20 and performs corresponding operations to complete the liquid preparation;

[0123] The detection seat 300 moves from the liquid preparation station to the sample detection station below the metal shielding cover 500;

[0124] The metal shielding cover 500 moves downward to cooperate with the detection seat 300;

[0125] The air pressure connection device 510 connected to the metal shielding cover 500 starts to provide pressure to drain the liquid in the impedance detection pool on the reagent kit 20 for impedance detection.

[0126] The POCT blood cell analyzer and the method of use thereof provided in this embodiment have a high degree of automation, and the pre-treatment and liquid preparation operations of the test liquid are all automatically completed inside the instrument, avoiding the randomness and errors of manual operations.

[0127] Please refer to the sixth embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a detection seat 300 and a metal shielding cover 500.

[0128] The detection seat 300 reciprocates between the loading station and the sample detection station, and is used to receive the reagent kit 20 provided with the impedance detection cell at the loading station, and the metal shielding cover 500 is provided at the sample detection station, and is used to cover the detection seat 300 when the detection seat 300 moves to the sample detection station to shield the electromagnetic signal. The POCT blood cell analyzer also includes a liquid preparation station; the liquid preparation station is located between the loading station and the sample detection station; or the liquid preparation station and the sample detection station are the same station.

[0129] The detection seat 300 is in a semi-enclosed shape with an opening at the top, and the metal shielding cover 500 is in a semi-enclosed shape with an opening at the bottom. The detection seat 300 is snap-fitted with the reagent kit 20. The detection seat 300 includes a main body 310 and a metal shielding seat (320, 321, 322) covering the outer periphery of the main body 310. The detection seat 300 is configured to be able to translate or rotate under the metal shielding cover 500, and the metal shielding cover 500 is configured to be able to move up and down in the vertical direction.

[0130] The POCT blood cell analyzer also includes a pressure building system, one end of which is provided with a pneumatic connection device 510 extending into the metal shielding cover 500 and moving synchronously with the metal shielding cover 500, and the reagent kit 20 is provided with a pressure application chamber 140 connected to the pneumatic connection device 510.

[0131] This embodiment also provides a method for using a POCT blood cell analyzer, the method comprising the following steps:

[0132] The detection seat 300 slides out or rotates out of the frame 301 to receive the reagent kit 20 at the loading station;

[0133] The detection seat 300 slides into or rotates into the frame 301 and moves to the liquid preparation station;

[0134] The pipette 400 performs corresponding operations on the reagent kit 20 at the liquid preparation station;

[0135] The metal shielding cover 500 covers the detection base 300 and performs impedance detection. Specifically, the detection base 300 first moves to the bottom of the metal shielding cover 500, and then the metal shielding cover 500 moves downward to cover the detection base 300.

[0136] The pipette 400 prepares the reagent kit 20 by liquid preparation, including: the pipette 400 absorbs the blood sample and reagent pre-installed in the reagent kit 20 and transfers them into the detection pool of the reagent kit 20, wherein the reagent includes a hemolytic agent and a diluent, etc.

[0137] The POCT blood cell analyzer and the method of use thereof provided in this embodiment have a high degree of automation, and the pre-treatment and liquid preparation operations of the test liquid are all automatically completed inside the instrument, avoiding the randomness and errors of manual operations.

[0138] Please refer to the seventh embodiment. Figures 1 to 13 This embodiment provides a detection seat 300, which includes a detection cavity 302 and a conductive support 303.

[0139] The detection chamber 302 is used to receive the reagent box 20 provided with an impedance detection cell, the reagent box 20 is provided with electrodes, and the conductive support 303 is used to electrically connect the electrodes. The electrodes are conductive pillars 122 provided on opposite sides of the reagent box 20.

[0140] like Fig.10 As shown, in one embodiment, the conductive support 303 includes a downwardly inclined spring sheet portion 304, and the spring sheet portion 304 is used to support and electrically connect the conductive column 122. The conductive support 303 also includes a fixed connection portion 305 integrally connected to the spring sheet portion 304. The shape of the fixed connection portion 305 is not limited, and it can be provided with a fixing hole for fastening and assembly by screws.

[0141] like Fig.11 As shown, in another embodiment, the conductive support 303 includes a fixed connection portion 305 connected in an integral manner and arranged vertically and a bent portion 306 (which may be a V-shaped bend or an arc-shaped bend) extending in a bent shape. When the conductive column 122 is assembled from top to bottom, the bent portion 306 is squeezed open. When the conductive column 122 is assembled in place, the bent portion 306 is reset and abuts against the conductive column 122. This form of conductive support 303 has good elastic deformation ability, is durable, and can maintain reliable electrical connection for a long time. The conductive support 303 can be one and arranged on one side of the conductive column 122, or two and respectively arranged on both sides of the conductive column 122 to further increase the reliability of the electrical connection. Specifically, the bent portion 306 may include a first bent portion 3061 and a second bent portion 3062 that are sequentially connected to the fixed connection portion 305. When the conductive column 122 is assembled from top to bottom, the first bent portion 3061 is squeezed open. When the conductive column 122 is assembled in place, the second bent portion 3062 abuts against the conductive column 122.

[0142] The detection seat 300 also includes a main body 310 and a metal shielding seat (320, 321, 322). The main body 310 is provided with a detection cavity 302. The side wall of the main body 310 is provided with an assembly groove 307 connected to the detection cavity 302. The conductive support 303 extends into the detection cavity 302 through the assembly groove 307. The metal shielding seat (320, 321, 322) is covered on the outer periphery of the main body 310 and is insulated from the conductive support 303. The metal shielding seat (320, 321, 322) includes side plates (320, 321) surrounding the main body 310 and a bottom plate (322) attached to the bottom of the main body 310.

[0143] The test kit 20 also includes an integrally connected or detachable optical detection pool. The detachable optical detection pool can be inserted through the rectangular insertion hole 107. The detection seat 300 also includes an optical detection component 330 corresponding to the optical detection pool. The optical detection component 330 includes a light-emitting component, a light-receiving component, and a detection cavity located between the light-emitting component and the light-receiving component. The angle between the axis of the light-emitting component and the axis of the light-receiving component ranges from 0 to 60 degrees, and can specifically be 0 degrees, 20 degrees, 30 degrees, 45 degrees, 60 degrees, etc.

[0144] The optical detection components 330 are divided into multiple groups, and the light source cavities of the multiple groups of optical detection components 330 are separated from each other by light-blocking walls 333 to avoid mutual interference of detection lights.

[0145] For example, multiple groups of optical detection components 330 may include two groups of laser detection components 331 and one group of LED light source detection components 332. A light-blocking wall 333 is provided between the detection cavities of the laser detection components 331. The light-blocking wall 333 is preferably blackened to have better light absorption effect and poorer light reflection effect, so as to avoid reflected light affecting normal optical detection.

[0146] The detection seat 300 also includes a Peltier 340, a heat sink 350 and a fan 360 which are sequentially arranged near the detection cavity of the laser detection component 331. The heat sink 350 includes a heat-absorbing substrate 351 bonded to the Peltier 340 and heat-dissipating fins 352 vertically connected to the heat-absorbing substrate 351. The fan 360 can be arranged at one end of the heat-dissipating fins 352 away from the heat-absorbing substrate 351. By arranging the Peltier 340, the heat sink 350 and the fan 360, the detection temperature can be controlled, and the detection result obtained by performing detection at a preset detection temperature is relatively more accurate.

[0147] A touch switch 334 is provided at the bottom of the detection chamber 302. The touch switch 334 can be used to obtain the loading status of the reagent kit 20. When the reagent kit 20 is installed, the touch switch 334 is pressed down, and when the reagent kit 20 is not installed, the touch switch 334 is in a pop-up state.

[0148] This embodiment further provides a POCT blood cell analyzer, which includes a pipette 400 and the aforementioned detection seat 300 and the pipette 400 disposed above the detection seat 300 . The pipette 400 is used to perform corresponding operations on the reagent kit 20 .

[0149] The conductive support 303 of the POCT blood cell analyzer and its detection base 300 provided in this embodiment includes a downwardly inclined spring portion 304, which has good adaptability and stability when electrically connected with the electrodes of the reagent kit 20, greatly reducing the possibility of poor contact.

[0150] An eighth embodiment, this embodiment provides a method for using a POCT blood cell analyzer, comprising the steps of:

[0151] In response to the reagent box 20 being placed in the detection seat 300, the pipette 400 is controlled to move to above the first mounting head accommodating pool of the reagent box 20 and descend to load the first mounting head, wherein the first mounting head may be a tip head 201;

[0152] Control the pipette 400 to move to the diluent reservoir 111 of the reagent kit 20 to transfer the diluent into the fore reservoir 120 (ie, the WBC detection reservoir and / or the RBC detection reservoir) for impedance detection;

[0153] Control the pipette 400 to move to the sample tube / sample dilution pool / other sample placement location to move the sample into the front pool 120, wherein the sample in the sample dilution pool is the diluted sample;

[0154] Bubbling and mixing or stirring the liquid to be tested in the fore cell 120;

[0155] Control the metal shielding cover 500 to cover the detection seat 300 to establish a connection between the back pool of the reagent kit 20 and the pressure building system;

[0156] Control the pressure building system to drain the test fluid from the front tank 120 into the rear tank;

[0157] The test liquid is detected by impedance detection method.

[0158] Among them, controlling the pipette 400 to move to above the first mounting head accommodating pool of the reagent kit 20 and descend to load the first mounting head includes: controlling the pipette 400 to move three-dimensionally relative to the first mounting head placement pool, so that the pipette 400 and the first mounting head are squeezed against each other to load the first mounting head.

[0159] Controlling the pipette 400 to move to the dilution pool 111 of the reagent kit 20 to move the dilution pool into the fore pool 120 for impedance detection and / or controlling the pipette 400 to move to the sample tube / sample dilution pool / other sample placement location to move the sample into the fore pool 120 includes: the pipette 400 makes three-dimensional movement relative to the fore pool 120 and uses the inner cavity of the first mounting head as a liquid transfer position to absorb the dilution liquid and / or the sample. The volume of the inner cavity of the first mounting head is generally 100 microliters to 1000 microliters, and generally does not exceed 4 / 5 of the volume when in use to avoid liquid entering the pipeline of the pressure building system to cause contamination.

[0160] Mixing the test liquid in the fore cell 120 includes the pipette 400 sucking and discharging the test liquid through the first mounting head; or the pipette 400 injecting bubbles into the test liquid through the first mounting head; or the pipette 400 stirring the test liquid through the first mounting head.

[0161] After the test liquid in the front pool 120 is mixed, the pipette 400 unloads the first mounting head to the first mounting head accommodating pool.

[0162] After the pipette 400 unloads the first mounting head into the first mounting head accommodating pool, the pipette 400 moves to above the second mounting head accommodating pool and descends to load the second mounting head.

[0163] After the pipette 400 moves to the second mounting head accommodating pool and descends to load the second mounting head, the following steps are performed:

[0164] The pipette 400 moves to the sample tube / sample dilution pool / other sample placement location to move the sample into the light detection pool;

[0165] The test liquid is detected by optical detection method.

[0166] After the pipette 400 moves to the second mounting head accommodating pool and descends to load the second mounting head, the following steps are performed:

[0167] The pipette 400 moves to the sample tube and moves the sample into the staining and lysis pool 602 for incubation and then moves it into the microfluidic detection chip 600;

[0168] The particles in the microfluidic detection chip 600 are detected by image detection method.

[0169] This embodiment also provides a POCT blood cell analyzer, which includes a detection base 300, a pipette 400, a pressure building system, a metal shielding cover 500, and a transmission component.

[0170] The detection seat 300 is used to receive the reagent kit 20, and the reagent kit 20 is provided with a front pool 120 and a rear pool connected by micropores and is equipped with a first mounting head, a diluent, and a sample;

[0171] The pipette 400 is used to load the first mounting head and transfer the diluent and the sample into the fore cell 120;

[0172] The metal shielding cover 500 is used to cover the detection seat 300, and the pressure building system is used to guide the liquid to be tested from the front tank 120 into the rear tank.

[0173] The transmission assembly is used to drive the detection seat 300, the pipette 400 and the metal shielding cover 500 to perform one-dimensional, two-dimensional or three-dimensional movement. The transmission assembly can be composed of a motor, a screw rod, a nut, a slide rail, a slider, a gear, a rack, a synchronous belt, etc.

[0174] The reagent kit 20 is also equipped with a second mounting head and an optical detection cell. The pipette 400 is also used to load the second mounting head and move the sample into the optical detection cell for optical detection.

[0175] Alternatively, the reagent kit 20 is also equipped with a second mounting head, a staining and lysis reagent, and a microfluidic detection sheet 600. The POCT blood cell analyzer also includes an image detection base 610 and an image detection device 620 that are arranged at intervals. The pipette 400 is also used to load the second mounting head and move the sample into the staining and lysis reagent for incubation, and then move the incubated sample into the microfluidic detection sheet 600, and perform image detection on the microfluidic detection sheet 600 through the image detection base 610 and the image detection device 620.

[0176] The POCT blood cell analyzer and the method of use thereof provided in this embodiment have a high degree of automation, and can complete automatic liquid preparation inside the instrument. The process operation consistency is good, and it can support impedance detection, optical detection, and image detection at the same time.

[0177] Please refer to the ninth embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a detection seat 300, a puncture head loading mechanism, a puncture head 204, and a pipette 400. The puncture head loading mechanism can be an independent mechanism or the same mechanism as the pipette 400.

[0178] The detection seat 300 is used to load the reagent kit 20. The reagent kit 20 is provided with a diluent pool and a hemolytic agent pool. The openings of the diluent pool and the hemolytic agent pool are provided with sealing films.

[0179] The puncture head 204 is used to puncture at least two sealing films on the reagent kit 20 loaded into the POCT blood cell analyzer one by one, and the puncture head loading mechanism is used to load the puncture head 204 .

[0180] The puncture head 204 is disposed on the reagent box 20 and / or the detection seat 300. The reagent box 20 and / or the detection seat 300 are provided with a placement position matching the puncture head 204. After being loaded by the pipette 400, the puncture head 204 moves synchronously with the pipette 400.

[0181] After penetrating the sealing film, the puncturing head 204 further swings horizontally, thereby enlarging the diameter of the through hole formed on the sealing film.

[0182] Preferably, at least two sealing films are located on the same straight line or the same arc, thereby making the trajectory of puncturing one by one relatively simple.

[0183] like Figure 7As shown, the puncture head 204 includes a main body 2042 and a sharp portion 2041, and the sharp portion 2041 is arranged at one end of the main body 2042. The cross section of the sharp portion 2041 can be in a straight line shape, a cross shape, a fenestration shape, or a Y shape. A flange portion 2044 is provided at one end of the main body 2042 away from the sharp portion 2041. The flange portion 2044 can facilitate the puncture head 204 to be hung on the puncture head receiving pool 104, and can also form a stable abutment fit with the outer sleeve 402.

[0184] Please refer to the tenth embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a detection seat 300 and a mounting head.

[0185] The detection seat 300 is used to load the reagent box 20. The reagent box 20 is provided with a diluent pool and a hemolytic agent pool. The openings of the diluent pool and the hemolytic agent pool are provided with sealing films. The mounting head is used to puncture the sealing films on the reagent box 20 loaded into the POCT blood cell analyzer one by one. In this embodiment, the mounting head is a tip head 201 or a dedicated puncture head 204, and the mounting head is arranged on the reagent box 20. The reagent box 20 is provided with a placement position matching the mounting head. The outer diameter of the puncture head 204 is larger than the outer diameter of the tip head 201. The opening formed by the puncture head 204 passing through the sealing film is larger than the diameter of the tip head 201 extending into the opening, thereby preventing the formation of a negative pressure cavity in the pool when the tip head aspirates liquid, thereby ensuring the accuracy of aspiration.

[0186] The POCT blood cell analyzer also includes a pipette 400 , and the mounting head moves along with the pipette 400 after being loaded by the pipette 400 .

[0187] At least two sealing films are located on the same straight line or the same arc line, so that the trajectory of puncturing one by one can be relatively simple. After the mounting head passes through the sealing film, it further swings horizontally to expand the diameter of the through hole on the sealing film.

[0188] In one embodiment, the tip head 201 can first pierce the first through hole with a first stroke, and then pierce the second through hole with a second stroke to aspirate the sample. The first stroke is smaller than the second stroke, thereby avoiding the formation of a negative pressure sealing cavity during aspiration that affects the accuracy of aspiration.

[0189] In another embodiment, the tip is lifted after piercing the through hole and then the sample is sucked, which can also avoid the formation of a negative pressure sealing cavity during the suction and affect the accuracy of the suction.

[0190] The puncture head 204 includes a main body 2042 and a sharp portion 2041 disposed at the end of the main body 2042 .

[0191] The cross section of the sharp portion 2041 is in a straight line shape, a cross shape, a pound shape, or a Y shape, etc. A flange portion 2044 is disposed at one end of the main body portion 2042 away from the sharp portion 2041 .

[0192] In this embodiment, the tip head 201 may be directly used for puncturing, or a dedicated puncturing head 204 may be used for puncturing.

[0193] Please refer to the eleventh embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a housing 10 , a pipette 400 , and a puncture head 204 .

[0194] The pipette 400 is disposed in the housing 10, and the pipette 400 includes an airway tube 401. The puncture head 204 includes a main body 2042 and a sharp portion 2041. The sharp portion 2041 is disposed at one end of the main body 2042, and the other end of the main body 2042 is provided with a receiving cavity 2045 for inserting the airway tube 401.

[0195] The sharp portion 2041 is used to puncture the sealing film on the reagent kit 20 when the POCT blood cell analyzer performs automated testing. The cross section of the sharp portion 2041 is in a straight line, a cross, a mitre or a Y shape, and a flange portion 2044 is provided at one end of the main body 2042 away from the sharp portion 2041 to facilitate hanging on the box body 100 of the reagent kit 20. The POCT blood cell analyzer also includes a detection seat 300, which is used to install the reagent kit 20. The reagent kit 20 is provided with a puncture head placement pool, and the flange portion 2044 is supported on the periphery of the puncture head placement pool.

[0196] The pipette also includes an outer sleeve 402 sleeved on the outer periphery of the airway tube 401, the lower end of the outer sleeve 402 abuts against the flange 2042, and the end of the main body 2042 close to the flange 2044 is provided with a plurality of ribs 2043 extending toward the sharp portion 2041 to improve the strength. The puncture head 204 can be integrally formed of metal or plastic material.

[0197] like Fig.12 As shown, this embodiment provides a reagent kit 20, which includes a plurality of pools, one of which is used to place the aforementioned puncture head 204.

[0198] The plurality of pools include a diluent pool 111 and / or a hemolytic agent pool 106 , and the diluent pool 111 and / or the hemolytic agent pool 106 are provided with a sealing membrane (not shown).

[0199] The pool position also includes a WBC detection pool and / or an RBC detection pool for performing impedance detection.

[0200] like Fig.12As shown, the test kit 20 also includes a microfluidic detection chip 600 , and the microfluidic detection chip 600 can perform image detection through an image detection base 610 and an image detection device 620 .

[0201] In this embodiment, the sealing film puncture operation may not require manual operation, and a dedicated puncture head 204 or tip head 201 may be used to achieve better consistency of operation.

[0202] Please refer to the twelfth embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a detection seat 300 and a pressure building component.

[0203] The detection seat 300 is used to install the test kit 20, and the test kit 20 includes an impedance detection cell for impedance detection. The impedance detection cell includes a front cell 120 and a rear cell connected by micropores. The pressure building component is connected to the front cell 120 and / or the rear cell gas path to provide pressure so that the test liquid in the front cell 120 flows to the rear cell through the micropores. It is also used to provide pressure to the front cell 120 or the rear cell before the test liquid is injected into the front cell 120 so that the micropores are unobstructed. When the pressure building component provides negative pressure, impedance detection can be performed. When the pressure building component provides positive pressure, the micropores can be backflushed to ensure the unobstructed micropores.

[0204] The pressure building component includes a syringe 220 and a pressure chamber 210 connected by an air path, and the syringe 220 is used to establish positive pressure or negative pressure for the pressure chamber 210. The pressure building component is used to provide airflow to achieve bubble mixing of the liquid to be tested or to make the micropores unobstructed, specifically, blowing or sucking air through the front pool 120 / back pool to make the micropores unobstructed.

[0205] The POCT blood cell analyzer includes a plurality of groups of selector valves, and the selector valves connect the pressure chamber 210 and the syringe 220 .

[0206] The POCT blood cell analyzer includes a metal shielding cover 500 matched with the detection seat 300, and the metal shielding cover 500 is provided with a gas pressure connection device 510 connected with the pressure chamber 210 or the syringe 220, and the gas pressure connection device 510 is used to connect with the rear pool or the front pool 120. The metal shielding cover 500 is configured to move in the vertical direction.

[0207] The POCT blood cell analyzer further includes a pipette 400 , which is disposed above the detection seat 300 and is used to perform corresponding operations on the reagent kit 20 .

[0208] The pipette 400 is connected to the gas path of the pressure building component through a pipeline. The pipette 400 is used to load the mounting head on the reagent kit 20 and perform corresponding operations through the mounting head.

[0209] This embodiment also provides a method for using a POCT blood cell analyzer, which includes the following steps:

[0210] The receiving reagent kit is loaded into the detection seat 300, the reagent kit includes an impedance detection cell for performing impedance detection, and the impedance detection cell includes a front cell 120 and a rear cell connected by micropores;

[0211] Before the test liquid is injected into the front pool 120, pressure is provided to the front pool 120 or the rear pool to make the micropores unobstructed. Specifically, it may include: providing pressure to the front pool 120 or the rear pool through the pressure in the pressure chamber 210 of the pressure building component to make the micropores unobstructed; or providing pressure to the front pool 120 or the rear pool through the syringe 220 of the pressure building component to make the micropores unobstructed.

[0212] Please refer to the thirteenth embodiment. Figures 1 to 13 The present embodiment provides a POCT blood cell analyzer, which includes a detection seat 300, a human-computer interaction module and a processor. The detection seat 300 is used to receive and load a test kit 20. The test kit 20 is provided with at least two types of project detection pools. The detection seat 300 is provided with an auxiliary detector for detecting the project corresponding to the project detection pool. The human-computer interaction module is connected to the detection seat 300 for communication and is used to select the detection project. The human-computer interaction module is a combination of a touch screen or a display screen 11 and a mechanical button. The processor is connected to the detection seat 300 and the human-computer interaction module for receiving the detection project and the detection signal of the auxiliary detector, and comparing whether the detection project and the detection signal correspond. If they correspond, the subsequent operation can be carried out normally. If they do not correspond, the correct test kit 20 needs to be replaced or the correct test project needs to be reselected. The project detection pool is detachably mounted on the test kit 20, and the auxiliary detector is used to detect whether there is a corresponding project detection pool.

[0213] The auxiliary detector includes an optical detection component 330, which includes a light-emitting component and a light-receiving component. The optical signal obtained by the light-receiving component is used to assist in determining whether the item detection pool on the reagent kit 20 corresponds to the selected detection item.

[0214] The auxiliary detector includes a touch switch 334, and the signal obtained by the touch switch 334 is used to assist in determining whether the item detection pool on the reagent kit 20 corresponds to the selected detection item.

[0215] The auxiliary detector includes an image recognition base 610 and an image recognition device 620 which are arranged relative to each other at intervals. The signals obtained by the image recognition base 610 and the image recognition device 620 are used to assist in determining whether the item detection pool on the reagent kit 20 corresponds to the selected detection item.

[0216] The POCT blood cell analyzer also includes a barcode scanner, which is used to obtain label information corresponding to the test kit 20. The label information may include name, type, production time and other information.

[0217] This embodiment also provides a method for using a POCT blood cell analyzer, comprising the following steps:

[0218] Select the items to be tested in the human-computer interaction module;

[0219] Obtaining the corresponding detection position information of the reagent kit 20;

[0220] The processor determines whether the detection position information corresponds to the selected detection item. If not, a corresponding prompt is output. If it corresponds, subsequent operations can be performed normally. If not, it is necessary to replace the correct reagent kit 20 or reselect the correct detection item.

[0221] In the step of obtaining the detection position information corresponding to the reagent kit 20, the detection position information corresponding to the reagent kit 20 includes a scattered light signal, a transmitted light signal or an electrical signal triggered by a micro switch.

[0222] Among them, the items to be tested include at least one of routine blood test, CRP test, SAA test, and blood cell classification test.

[0223] This embodiment also provides a method for using a POCT blood cell analyzer, including:

[0224] Acquiring detection information of items supported by the test kit 20 through the auxiliary detector;

[0225] Select the items to be tested in the human-computer interaction module;

[0226] It is determined whether the item detection information supported by the reagent kit 20 corresponds to the selected item to be detected. If not, a corresponding prompt is output.

[0227] The solution provided in this embodiment compares the items that the user wants to detect with the items that the test kit can actually support before the test, which can better prevent mistakes.

[0228] Please refer to the 14th embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a housing 10 , a detection seat 300 , a pipette 400 , a first catheter 211 and an anti-bending member 212 .

[0229] The detection seat 300 can be extended into or out of the housing 10, and is used to receive the reagent kit 20 provided with an impedance detection cell. The detection seat 300 is provided with a power supply member (eg Fig. 9 The conductive support 303 shown in FIG.

[0230] The pipette 400 is disposed in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent kit 20 .

[0231] One end of the first conduit 211 is connected to the air path of the pipette 400, and the other end of the first conduit 211 is connected to the pressure building system (eg Figure 5 The pressure building system may include at least one of the syringe 220, the pressure chamber 210, the solenoid valves (SV1, SV2, SV3), the multi-way connector, and the manifold. The anti-bending member 212 is combined with the first conduit 211 to prevent the first conduit 211 from excessively bending and blocking the internal air guide channel of the first conduit 211. The inner diameter of the first conduit 211 is relatively thin, generally 0.5 mm to 1.5 mm, and is prone to excessive bending and blocking in actual use.

[0232] The anti-bending member 212 may be a sleeve sleeved on the outer periphery of the first conduit 211, and the hardness of the sleeve is less than or equal to the hardness of the first conduit 211. Of course, the hardness of the sleeve may also be greater than the hardness of the first conduit 211. After the outer periphery of the first conduit 211 is sleeved with the sleeve, the overall hardness of the two is relatively increased, the hardness of the first conduit 211 is relatively large, and the deformation of the first conduit 211 itself is relatively small when the air pressure in the first conduit 211 changes, thereby improving the accuracy of sample suction. The wall thickness of the sleeve may be greater than or equal to the wall thickness of the first conduit 211, and the bending resistance of the sleeve may be less than or equal to the bending resistance of the first conduit 211, thereby reducing the wear of the sleeve on the first conduit 211, and preventing the first conduit 211 from excessive bending and blocking the internal air guide channel of the first conduit 211. The cross section of the sleeve is annular, or the cross section of the sleeve is C-shaped. The anti-bending member 212 may also be a spring sleeved on the outer periphery of the first conduit 211. The anti-bending member 212 may also be a band wrapped around the outer circumference of the first conduit 211 , and the band may be a cloth band, a plastic band, or a metal band.

[0233] The two ends of the first conduit 211 are respectively connected to the pipette 400 and the second syringe 223. The ends of the first conduit 211 are provided with joints, which can be arranged at one end or both ends of the first conduit 211. The joints can be clamps, clamp joints, flanged joints, straight joints or threaded joints for air path sealing connection.

[0234] The POCT blood cell analyzer provided in this embodiment can effectively avoid the situation of air path obstruction and improve the stability of the instrument because the anti-bending member 212 is provided on the periphery of the relatively thin first catheter 211.

[0235] Please refer to the fifteenth embodiment. Figures 1 to 13This embodiment provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400, a pressure building system, a first catheter 211 and a second catheter 221.

[0236] The detection seat 300 can be extended into or out of the housing 10, and is used to receive the reagent kit 20 provided with an impedance detection cell. The detection seat 300 is provided with a power supply member (eg Fig. 9 The conductive support 303 shown in FIG.

[0237] The pipette 400 is disposed in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent kit 20 .

[0238] The two ends of the first conduit 211 are connected to the pipette 400 and the second syringe 223 respectively. The second conduit 221 connects the syringe 220, the pressure chamber 210, and the solenoid valves (SV1, SV2, SV3). Figure 5 The inner diameter of the T3 pipeline in the second conduit 221 is less than or equal to that of the second conduit 221 (i.e. Figure 5 The inner diameter of the pipeline (except T3 and T4 in the middle figure), the hardness of the first conduit 211 is greater than the hardness of the second conduit 221. Among them, the first conduit 211 mainly controls the accuracy of sample suction or sample pushing, requiring the error caused by gas compression to be small, so the hardness is large and the inner diameter is relatively small, while the second conduit 221 mainly builds pressure, requires fast speed, and the inner diameter can be relatively large.

[0239] The ratio of the inner diameter of the first conduit 211 to the inner diameter of the second conduit 221 may be greater than 0.5 and less than or equal to 1. The ratio of the length of the first conduit 211 to the length of the second conduit 221 is greater than 1 and less than or equal to 1.5, and the first conduit 211 is relatively long to facilitate the installation of a joint (such as a clamp joint). In other words, the first conduit 211 is relatively thin and hard, and the second conduit 221 is relatively thick and soft.

[0240] like Figure 5 As shown, the POCT blood cell analyzer further includes an anti-bending member 212 , which is combined with the first conduit 211 to prevent the first conduit 211 from being excessively bent and thus blocking the internal air-guiding channel of the first conduit 211 .

[0241] The anti-bending member 212 may be a sleeve or a spring sleeved on the outer periphery of the first conduit 211. The hardness of the sleeve is less than or equal to the hardness of the first conduit 211. Of course, the hardness of the sleeve may also be greater than the hardness of the first conduit 211. After the sleeve is sleeved on the outer periphery of the first conduit 211, the overall hardness of the two is relatively increased. The hardness of the first conduit 211 is relatively large. When the air pressure in the first conduit 211 changes, the deformation of the first conduit 211 itself is relatively small, thereby improving the accuracy of the suction and exhalation operation. The wall thickness of the sleeve may be greater than or equal to the wall thickness of the first conduit 211, and the bending resistance of the sleeve may be less than or equal to the bending resistance of the first conduit 211. The cross-section of the sleeve is annular or C-shaped. The anti-bending member 212 may also be a band wrapped around the outer periphery of the first conduit 211. The band is a cloth band, a plastic band or a metal band.

[0242] The hardness of the first conduit 211 is greater than or equal to the hardness of the second conduit 221. The hardness of the first conduit 211 is relatively large. When the air pressure in the first conduit 211 changes, the deformation of the first conduit 211 itself is relatively small, thereby improving the accuracy of sample suction; the wall thickness of the first conduit 211 is less than or equal to the wall thickness of the second conduit 221; the bending resistance of the first conduit 211 is less than or equal to the bending resistance of the second conduit 221. This embodiment selects the inner diameter and hardness according to two different requirements, which can meet the requirements in a targeted manner and realize the accuracy and efficiency of the instrument. In addition, an anti-bending member 212 is designed for the thinner first conduit 211, which can better avoid the situation of air path blockage, further improving the stability of the instrument.

[0243] Please refer to the sixteenth embodiment. Figures 1 to 14 This embodiment provides a method for using a POCT blood cell analyzer, which may include the following steps:

[0244] S10, receiving a power-on trigger signal, which may be specifically a user pressing a mechanical button to turn on the power;

[0245] S11, perform hardware self-test, that is, test and feedback the voltage and current outputted by each port on the circuit board;

[0246] S12, determining whether the pipette is loaded with a mounting head, which can be determined by detecting elements such as a transmission optical coupler or a reflection optical coupler, and the mounting head can be a tip head or a puncture head;

[0247] S18, if yes, the pipette unloads the mounting head, and the unloading location may be a specific location inside the instrument, or may be a certain pool position on the reagent kit 20 inside the instrument;

[0248] S13, if not, the whole machine is initialized, that is, each moving part is reset to zero or confirmed to zero and the pressure is cleared. After the initialization is completed, the reagent kit 20 can be received and loaded, and fully automated sample pre-processing (automatic mixing of samples and corresponding reagents) is performed in the POCT blood cell analyzer. After the automated sample pre-processing is completed, the sample is tested.

[0249] Among them, after the pipette unloads the mounting head, it includes:

[0250] S19, the detection seat 300 is taken out of the chamber for the reagent kit 20 to be taken out. In this case, usually the power is accidentally cut off during the last use, and the installation head and the reagent kit 20 are still left inside the instrument;

[0251] S20, the detection seat 300 returns to the warehouse and performs the whole machine initialization step. This step is to ensure that there are no external components (installation head and reagent kit 20) in the machine, and then the subsequent power-on process actions will not be affected by the presence of the tip head or reagent kit 20.

[0252] The step of determining whether the pipette is loaded with an installation head also includes: determining whether a reagent kit 20 is installed in the detection seat 300. Generally speaking, if there is an accidental power outage, both the reagent kit 20 and the installation head will be present inside the instrument. Therefore, it is possible to determine whether only the installation head is present inside the instrument, or it is possible to determine whether both the installation head and the reagent kit 20 are present inside the instrument at the same time. The determination of the reagent kit 20 can be made by means of a touch switch inside the detection seat 300 or the like.

[0253] After the whole machine initialization step, it also includes:

[0254] S14, perform pressure detection to determine whether the pressure in the gas pipeline reaches the threshold range. The POCT blood cell analyzer provided by the present application may be equipped with one, two or more pressure chambers 210, and establish positive or negative pressure through a syringe and reach a certain pressure value range. Specifically, after initialization, a pressure self-test process is performed. The pressure self-test requires a certain value of negative pressure to be established. Here, the pressure can be -25 to 30 kPa. The pressure building process relies on a solenoid valve, a syringe, a pressure sensor, a pressure chamber 210 and a gas pipeline. The pressure building process is as follows: During the pressure building process, the pressure sensor always detects the pressure of the pressure chamber 210. First, the syringe motor drives the syringe to pull outward at the maximum speed, and at the same time opens the solenoid valve to establish a negative pressure operation. At this time, the syringe is directly connected to the pressure chamber 210, and there is no other ventilation port opened in the pressure chamber 210. Negative pressure can be established when the syringe is pulled out. If the syringe motor reaches its maximum stroke, that is, the syringe has been pulled to the maximum range, but the target pressure has not been built, the solenoid valve is closed. At this time, the syringe is initialized at the maximum speed and the maximum distance. After the initialization is completed, the solenoid valve is opened and the syringe is continued to be pulled out to build pressure until the target pressure is reached. If the target pressure has not been reached after the pressure building period, a pressure building failure is reported. At this time, the pressure building is completed. The pressure self-test continues. When the target pressure is built, if the pressure sensor detects normally, the solenoid valve is opened to connect the interior of the pressure chamber 210 to the air to release the negative pressure. After the pressure self-test, a simple blank test is performed. There is no need to place the test kit 20. The signal under no-load condition is directly measured to ensure that the test performance of the POCT blood cell analyzer is normal. Next, the main measurement process is carried out.

[0255] S15, perform signal detection, including judging the detection signal after the electromagnetic shielding components are matched and then judging the electromagnetic shielding effect and judging whether the current and voltage signals are within the threshold range, wherein the signal detection may include various photoelectric signals, etc., and also includes simulating impedance method detection after the metal shielding cover and the metal shielding seat are combined and closed, and judging the shielding effect of the metal shielding cover and the metal shielding seat or the signal quality of the circuit board card itself according to the output results.

[0256] S16, enter the standby state, at this time you can choose to shut down or choose to perform detection.

[0257] Specifically, the step of entering the standby state includes:

[0258] S17, receiving a shutdown trigger signal, that is, the user presses the shutdown button. In order to prevent misoperation, it can be set that after the user presses the shutdown button, the screen displays whether the shutdown is confirmed;

[0259] S21, judging whether the reagent kit 20 is present, which can be done by using a touch switch in the detection seat 300;

[0260] S22, if the reagent kit 20 is determined to be OK, further determine the state of the door;

[0261] S24, if it is determined that the door is in the open state, a prompt is given to take out the reagent kit 20 and the process returns to the step of determining whether the reagent kit 20 is present;

[0262] S23, if it is determined that the door is in a closed state, the door is opened and the reagent kit 20 is prompted to be taken out, and then the process returns to the step of determining whether the reagent kit 20 is present;

[0263] S25, if it is determined that the reagent kit 20 is not present, further determining the state of the door;

[0264] S26, if it is determined that the door is open, close the door and prompt to turn off the power;

[0265] S27, if it is determined that the door is in a closed state, a prompt is given to turn off the power.

[0266] The steps to enter standby state include:

[0267] S31, receiving sample ID settings and measurement mode settings. Specifically, the screen may provide options such as mode settings, and provide some optional items for the user to choose, such as CRP testing, SAA testing, blood testing, etc.;

[0268] S32, receiving a start test trigger signal, that is, a touch button or a physical button may be provided on the screen or on the instrument to generate a start test trigger signal;

[0269] S33, a pop-up window is displayed to check whether the information to be tested (such as reagent information, or code scanning comparison / IC card reading comparison) matches the measurement mode. If not, click Cancel (S46) and return to step S31 to receive the sample ID setting and the measurement mode setting;

[0270] S34, if there is a match, click confirm;

[0271] S35, automatically open the door;

[0272] S36, a prompt to insert the test kit may be provided through a pop-up window or other means;

[0273] S37, click OK after inserting the reagent kit;

[0274] S38, check whether the reagent kit 20 is present, if not, return to step S36, and prompt to put in the reagent kit 20;

[0275] S39, if there is, the door will be automatically closed;

[0276] S40, performing an automated liquid preparation test. The specific process of the automated liquid preparation test may refer to the aforementioned embodiment.

[0277] S41, displaying the test results, which may be specifically displayed on a display screen or outputted by a printing device;

[0278] S42, click OK;

[0279] S43, automatically opening the door and prompting to take out the reagent kit 20;

[0280] S44, the user takes out the reagent kit 20 and clicks OK;

[0281] S45, automatically close the door and enter standby mode.

[0282] The method of using the POCT blood cell analyzer provided in this embodiment is reasonable and orderly, and can quickly perform single item detection or multiple items detection at the same time.

[0283] This embodiment also provides a POCT blood cell analyzer, including a housing 10 , a detection seat 300 and a pipette 400 .

[0284] The detection seat 300 can extend into or out of the shell 10, and is used to receive the reagent kit 20 and reciprocate between the reagent kit 20 loading station and the sample detection station; the pipette 400 is arranged in the shell 10 and located above the detection seat 300, and is used to perform automated sample pre-processing on the reagent kit 20.

[0285] The test kit 20 includes a front pool 120 and a rear pool connected by micropores. The POCT blood cell analyzer also includes a pressure building system, which is arranged in the housing 10 and is used to make the test liquid flow from the front pool 120 to the rear pool for impedance detection.

[0286] The POCT blood cell analyzer also includes a metal shielding seat (320, 321, 322) for covering the detection seat 300, the test kit 20 includes a pressure action chamber 140 connected to the back pool, the metal shielding seat (320, 321, 322) is provided with a gas pressure connection device 510 connected to the pressure building system, and when the metal shielding seat (320, 321, 322) is covered with the detection seat 300, the gas pressure connection device 510 is sealed and connected to the pressure action chamber 140.

[0287] The pipette 400 is provided with a detection member (eg Figure 6 The optical coupler 405 shown in the figure) is used to install the mounting head installed on the reagent box 20. The pipette 400 includes an air tube 401 and an outer sleeve 402 which is sleeved on the outer periphery of the air tube 401. The air tube 401 is used to load the mounting head installed on the reagent box 20. When the air tube 401 is loaded with the mounting head, the air tube 401 is inserted into the mounting head so that the mounting head pushes the outer sleeve 402 to change its position along the axial direction of the air tube 401. The detection component is used to detect the position of the outer sleeve 402 and then determine whether the mounting head is sleeved on the air tube 401.

[0288] The pipette 400 further includes a head retracting mechanism (refer to the aforementioned blocking member 4031 ), which is used to stop the outer sleeve 402 from rising when the air guide tube 401 rises so that the outer sleeve 402 can unload the mounting head.

[0289] The head-retracting mechanism is an electromagnet, a motor, or a U-shaped baffle plate arranged axially or radially relative to the air guide tube 401 .

[0290] The pressure building system includes a pressure chamber 210, a first syringe 222 and a second syringe 223 which are arranged in linkage. The first syringe 222 is used to establish positive pressure or negative pressure for the pressure chamber 210. The second syringe 223 is connected to the pipette 400 and is used to absorb reagents and / or samples by using the inner cavity of the mounting head as a liquid transfer position when the pipette 400 is equipped with a mounting head.

[0291] The POCT blood cell analyzer includes a first catheter 211 and a second catheter 221 . The first catheter 211 is connected between the second syringe 223 and the pipette 400 . The second catheter 221 is used to connect the syringe and the pressure chamber 210 . The inner diameter of the first catheter 211 is smaller than the inner diameter of the second catheter 221 .

[0292] An anti-bending member 212 is disposed on the outer periphery of the first conduit 211 . The anti-bending member 212 is a sleeve, a spring or a binding band.

[0293] The present embodiment also provides a POCT blood cell analyzer, which includes a detection seat 300, a pipette 400 arranged above the detection seat 300, and a pressure building system. The detection seat 300 is used to receive the reagent kit 20. The reagent kit 20 is provided with a front pool 120 and a rear pool connected by micropores and is equipped with reagents, samples, and an installation head. The pressure building system includes a pressure chamber 210, an air pressure connecting device 510, a first syringe 222, a second syringe 223, a driving member 224, a first solenoid valve SV1, a second solenoid valve SV2, and a third solenoid valve SV3.

[0294] The pneumatic connection device 510 is connected to the pressure chamber 210, and is used to apply positive pressure or negative pressure to the front pool 120 or the rear pool to realize the flow of liquid in the front pool 120 to the rear pool; the first syringe 222 is connected to the pressure chamber 210 / pipette 400, and is used to establish positive pressure or negative pressure in the pressure chamber 210, and is also used to assist the pipette 400 in pipetting, or to bubble and mix the liquid in the front pool 120; the second syringe 223 is connected to the pipette 400 for pipetting; the driving member is used to drive the first syringe 222 and the second syringe 223 at the same time; the first solenoid valve SV1 selectively connects the first syringe 222 with the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively connects the first solenoid valve with the pressure chamber 210 and the outside atmosphere; the third solenoid valve SV3 selectively connects the pneumatic connection device with the pressure chamber 210.

[0295] The present application also provides a test kit 20, which includes a box body 100, a tip head accommodating pool (101, 102, 103), an installation head accommodating pool, a sample accommodating pool 105, a hemolytic agent pool 106, a rectangular insertion hole 107, a circular insertion hole 108, a front pool 120, a mounting cavity, an assembly seat 160, a microporous sheet 170, a front pool 120 electrode 121, a rear pool electrode 165, a pressure application chamber 140, a tip head (201, 202, 203), a puncture head 204, a sample tube 205, a hemolytic agent container 206, a first pool body 207, a second pool body 208, etc.

[0296] The following will provide a detailed description based on various embodiments of the kit 20 .

[0297] Please refer to the seventeenth embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a microporous sheet 170, which includes a sheet body 171. The sheet body 171 is provided with micropores 172 that allow cells to pass through one by one. The micropores 172 can be made into different specifications according to cells of different particle sizes. The sheet body 171 is a plastic sheet body or a ceramic sheet body. The mechanical strength of the plastic sheet body or the ceramic sheet body is relatively weak. The embodiment of the present application further provides a reinforcement part 173 on the sheet body 171 to ensure the mechanical strength of the microporous sheet 170, and at the same time has the effect of convenient installation. When the microporous sheet 170 is installed, the micropores 172 are not easily contaminated or worn by contact. The material cost of the plastic sheet body or the ceramic sheet body is relatively cheap, and it can be used as a disposable product without the need to use expensive materials that can be repeatedly cleaned and used.

[0298] The sheet body 171 has a first surface 175 and a second surface 176 opposite to each other. The first surface 175 and / or the second surface 176 is provided with a reinforcement portion 173 .

[0299] The reinforcing portion 173 can be arranged close to the edge of the sheet body 171 . The reinforcing portion 173 can be a structure such as a convex ring. The convex ring can be a continuous integral convex ring or a convex ring formed by multiple convex hulls. The outer edge of the convex ring overlaps or does not overlap with the outer edge of the sheet body 171 .

[0300] The convex ring is connected to the first surface 175 and / or the second surface 176 of the sheet body 171 via a vertical surface, an inclined surface or an arc surface, wherein the inclined surface or the arc surface can further reduce sample residue and improve detection accuracy.

[0301] The first surface 175 of the sheet body 171 is provided with a concave limiting drainage portion 174 around the micropore 172 . The concave limiting drainage portion 174 may be spherical or conical.

[0302] The ratio of the thickness of the convex ring to the thickness of the sheet 171 is 0.2-2. Preferably, the ratio of the width of the convex ring to the radius of the sheet 171 is less than or equal to 1. If the thickness of the convex ring is too small, it cannot play a good role in strengthening the mechanical strength. If the thickness of the convex ring is too large, it will waste materials. If the thickness of the convex ring is too large, the wall thickness of the micropores 172 of the microporous sheet 170 is also difficult to control, resulting in a more complicated manufacturing process. The ratio of the width of the convex ring to the radius of the sheet 171 is 0.2-0.8. Similarly, if the width of the convex ring is too small, it cannot play a good role in strengthening the mechanical strength. If the width of the convex ring is too large, the wall thickness of the micropores 172 of the microporous sheet 170 is also difficult to control, resulting in a more complicated manufacturing process. If the width of the convex ring is too large (for example, close to the radius of the sheet 171), the axial channel of the micropore 172 will become longer, which will further cause the sample particles to be tested to flow back, which will affect the accuracy of the detection when the cells pass through.

[0303] The microporous sheet 170 provided in this embodiment can improve the mechanical strength of the microporous sheet 170 by providing a reinforcing portion 173 to prevent the microporous sheet 170 made of plastic or ceramic material from being easily deformed during assembly. This embodiment also provides a reagent kit 20 including the aforementioned microporous sheet 170. The specific structure of the reagent kit 20 is referred to below.

[0304] Please refer to the eighteenth embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a reagent kit 20 , which includes a box body 100 , an assembly seat 160 , and a microporous sheet 170 .

[0305] The box body 100 includes a fore cell 120. The box body 100 is provided with fore cell 120 electrodes 121 corresponding to the fore cell 120. The fore cell 120 can be provided with two groups, which are respectively used for cooperating with WBC (white blood cell) detection and RBC (red blood cell) detection.

[0306] like Fig.18As shown, the assembly seat 160 is connected to the box body 100 , and the assembly seat 160 is provided with an axial drainage cavity 167 (the axial drainage cavity 167 may also be referred to as a back cell) and a back cell electrode 165 extending toward the axial drainage cavity 167 .

[0307] like Figure 1 , Figure 2 , Fig.18 As shown, the microporous sheet 170 is provided with micropores 172 that allow cells to pass through one by one. The microporous sheet 170 is arranged between the front pool 120 and the axial drainage chamber 167. The front pool 120 and the axial drainage chamber 167 are connected through the micropores 172. The front pool 120 electrode 121 and the rear pool electrode 165 are respectively spaced apart on both sides of the microporous sheet 170.

[0308] The assembly seat 160 is detachably connected to the box body 100 .

[0309] In a specific embodiment, the front cell 120 electrode 121 can be integrally injection molded or detachably connected with the box body 100. The rear cell electrode 165 can be integrally injection molded or detachably connected with the assembly seat 160, the front cell 120 electrode 121 and / or the rear cell electrode 165 are columnar electrodes, and the length of the rear cell electrode 165 is greater than or equal to the length of the front cell 120 electrode 121. The microporous sheet 170 can be integrally injection molded or detachably connected with the box body 100, or the microporous sheet 170 can be integrally injection molded or detachably connected with the assembly seat 160.

[0310] The box body 100 is provided with a mounting cavity 130 , and the assembly seat 160 is snap-fitted, threaded, interference-fitted, laser-welded or adhesively-fitted with the mounting cavity 130 .

[0311] The box body 100 and / or the assembly seat 160 are made of plastic. The front cell 120 electrode 121 is embedded in the box body 100 and is flush with, protrudes or recessed in the outer surface of the box body 100. The rear cell electrode 165 is embedded in the assembly seat 160 and is flush with, protrudes or recessed in the outer end surface of the assembly seat 160. The outer ends of the front cell 120 electrode 121 and the rear cell electrode 165 (i.e., the two ends away from each other) are used to connect the working voltage, and the inner ends of the front cell 120 electrode 121 and the rear cell electrode 165 (i.e., the two ends close to each other) are in contact with the sample liquid to be tested. The axial drainage cavity 167 will be filled with the sample liquid to be tested during detection.

[0312] The reagent kit 20 further includes an inner sealing ring 164 , which is disposed between the microporous sheet 170 and the forechannel 120 , specifically between the first surface 175 of the microporous sheet 170 and the forechannel 120 , so that the sample liquid in the forechannel 120 can only enter the axial drainage cavity 167 through the micropores 172 .

[0313] The reagent kit 20 further includes an outer sealing ring 166 , which is disposed between the assembly seat 160 and the free end of the mounting cavity 130 .

[0314] In one embodiment, the inner sealing ring 164 , the outer sealing ring 166 , the microporous sheet 170 and the assembly seat 160 are separate structural components.

[0315] In another embodiment, the inner sealing ring 164, the outer sealing ring 166, the microporous sheet 170 and the assembly seat 160 can be an integrated structural component to reduce the number of assembly parts, reduce assembly difficulty, and save assembly time. Among them, the inner sealing ring 164 and the outer sealing ring 166 can be injection molded using a secondary injection molding process and a relatively soft plastic material.

[0316] In another embodiment, the inner seal ring 164, the outer seal ring 166 and the assembly seat 160 are an integrated structure, and the microporous sheet 170 is detachably connected to the integrated structure, which can reduce the number of assembly parts and ensure the manufacturing accuracy and yield rate of the microporous sheet 170. Among them, the inner seal ring 164 has a certain flexibility. When the microporous sheet 170 is installed with the integrated structure, it can be installed to the rear of the inner seal ring 164 through the flexibility of the inner seal ring 164, and the inner seal ring 164 still seals the first surface 175 of the microporous sheet 170.

[0317] The reagent kit 20 provided in this embodiment has a novel structure and is easy to assemble.

[0318] Please refer to the nineteenth embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides an assembly seat 160 , which includes an assembly tube 168 and a back cell electrode 165 .

[0319] The assembly cylinder 168 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611 (see Fig.16 ), the radial liquid outlet groove 1611 is used to discharge the gas or liquid in the axial drainage cavity 167; the rear cell electrode 165 is connected to the assembly tube 168 and extends to the axial drainage cavity 167.

[0320] The inner end of the radial liquid outlet groove 1611 and the inner end of the assembly tube 168 are the first preset distance, and the inner end of the rear cell electrode 165 and the inner end of the assembly tube 168 are the second preset distance, and the first preset distance is less than or equal to the second preset distance. When the first preset distance is less than the second preset distance, the bubbles in the axial drainage cavity 167 are more easily discharged from the axial drainage cavity 167. If bubbles remain in the axial drainage cavity 167, the detection accuracy will be affected. Wherein, the inner end is based on the interior of the box body 100 as a reference, and when the front cell 120 is used as a reference, the end pointing to the front cell 120 is the inner end, and the end away from the front cell 120 is the outer end. For example, the inner end of the installation cavity 130 is connected to the front cell 120 through the through hole 132, and the outer end of the installation cavity 130 is an open end for receiving the assembly seat 160 for loading.

[0321] The axial length of the radial liquid outlet groove 1611 in the axial drainage cavity 167 is greater than or equal to the length of the rear cell electrode 165 extending into the axial drainage cavity 167. The inner end of the rear cell electrode 165 may or may not extend into the axial drainage cavity 167, that is, the inner end of the rear cell electrode 165 may protrude, be flush with, or be recessed in the cavity bottom surface of the axial drainage cavity 167.

[0322] Furthermore, the outer surface of the assembly cylinder 168 is provided with a recessed area connected to the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 can allow the gas or liquid in the axial drainage chamber 167 to flow out through the radial liquid outlet groove 1611 directly above and then flow out through the guide groove 1612 obliquely above. Thus, the two assembly seats 160 can share a pressure chamber 140 connected to the two assembly seats 160. The pressure chamber 140 passes through the through hole 133 (which can be Fig.17 The fan-shaped structure shown in FIG. 1 is connected to the installation cavity 130 and is further connected to the axial drainage cavity 167 through the guide groove 1612 and the radial liquid outlet groove 1611 in sequence.

[0323] The inner end of the assembly tube 168 is provided with a microporous sheet 170 that allows cells to pass through one by one. The specific structure of the microporous sheet 170 can refer to the above-mentioned embodiment.

[0324] The distance from the inner end of the rear cell electrode 165 to the microporous sheet 170 is a third preset distance, which is 0.2-2 times the axial length of the axial drainage cavity 167. Experimental verification shows that within this range, better signal accuracy can be obtained when performing impedance detection.

[0325] A sink 1613 is provided at the inner end of the assembly tube 168 , the microporous sheet 170 is cooperatively connected to the sink 1613 , and the reinforcement portion 173 of the microporous sheet 170 abuts against the sink 1613 .

[0326] like Fig.16As shown, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 which are integrally connected. The rear pool cylinder 161 is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced apart and sleeved on the outer periphery of the rear pool cylinder 161.

[0327] The radial liquid outlet groove 1611 may be in the shape of a circular hole, a waist-shaped hole or a rectangular hole.

[0328] The present application also provides a test kit 20, which includes a box body 100 and the aforementioned assembly seat 160, the box body 100 is provided with a front pool 120 and an installation cavity 130 that are connected, the assembly cylinder 168 is connected to the installation cavity 130, and the front pool 120 is connected to the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170.

[0329] The reagent kit 20 and its assembly seat 160 provided in this embodiment can facilitate the discharge of bubbles in the assembly tube 168 by limiting the distance between the inner end of the radial liquid outlet groove 1611 and the inner end of the rear cell electrode 165 and the inner end of the assembly tube 168, thereby preventing bubbles from remaining in the assembly tube 168 and affecting the detection accuracy during impedance detection.

[0330] Please refer to the 20th embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides an assembly seat 160 , which includes an assembly tube 168 and a rear cell electrode 165 . The rear cell electrode 165 is a columnar electrode, and the rear cell electrode 165 is connected to the assembly tube 168 .

[0331] The assembly cylinder 168 is provided with a radial liquid outlet groove 1611 and a buckling portion 1631, and the buckling portion 1631 is aligned with the radial liquid outlet groove 1611. The buckling portion 1631 can be a buckling hole or a buckling protrusion. Correspondingly, the outer surface of the mounting cavity 130 is provided with a buckle block 131 corresponding to the buckling hole. The opening directions of one buckling portion 1631 and the radial liquid outlet groove 1611 are consistent and aligned, which can facilitate demolding from the same direction during injection molding.

[0332] The assembly tube 168 is provided with an axial drainage cavity 167 , which is communicated with the radial liquid outlet groove 1611 , and the rear cell electrode 165 extends toward the axial drainage cavity 167 .

[0333] The rear cell electrode 165 can be embedded in the assembly seat 160 by injection molding and flush with, protruding from, or recessed from the outer end surface of the assembly seat 160 .

[0334] In the embodiment of the present application, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 that are integrally connected. The rear pool cylinder 161 is provided with a radial liquid outlet groove 1611 and an axial drainage cavity 167, and the end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163.

[0335] The outer cylinder 163 may extend a plurality of positioning protrusions 1632 spaced apart from each other along the axial direction, and the buckling portion 1631 is disposed corresponding to the positioning protrusions 1632 .

[0336] There is a first preset distance between the inner end of the radial liquid outlet groove 1611 and the inner end of the assembly tube 168, and there is a second preset distance between the inner end of the rear cell electrode 165 and the inner end of the assembly tube 168. The first preset distance is less than or equal to the second preset distance.

[0337] The rear cell electrode 165 and the assembly tube 168 may be integrally formed or detachably connected.

[0338] A sink 1613 is provided at the inner end of the rear cell tube 161 for assembling a microporous sheet 170 that allows cells to pass through one by one.

[0339] The present application also provides a test kit 20, which includes a box body 100 and the aforementioned assembly seat 160, the box body 100 is provided with a front pool 120 and an installation cavity 130 that are connected, the assembly cylinder 168 of the assembly seat 160 is connected to the installation cavity 130, and the front pool 120 is connected to the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170.

[0340] The reagent kit 20 and its assembly seat 160 provided in this embodiment can facilitate the demolding operation after mold forming by aligning the radial liquid outlet groove 1611 and the buckling portion 1631.

[0341] Please refer to the twenty-first embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides an assembly seat 160 , which includes an assembly tube 168 and a rear cell electrode 165 .

[0342] The assembly cylinder 168 is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611 which are connected to each other. The outer surface of the assembly cylinder 168 is provided with a recessed area connected to the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 can make the gas or liquid in the axial drainage chamber 167 flow out through the radial liquid outlet groove 1611 (for example, directly above) and then flow smoothly to the pressure action chamber 140 through the guide groove 1612 (for example, obliquely above). Thus, the two assembly seats 160 can share a pressure action chamber 140 connected to the two assembly seats 160. The pressure action chamber 140 passes through the through hole 133 (which can be Fig.17The fan-shaped structure shown in FIG. 1 is connected to the installation cavity 130 and is further connected to the axial drainage cavity 167 through the guide groove 1612 and the radial liquid outlet groove 1611 in sequence.

[0343] The rear cell electrode 165 is connected to the assembly tube 168 and extends to the axial drainage cavity 167 .

[0344] The guide groove 1612 is arranged along the outer surface of the assembly tube 168 in the circumferential direction. The guide groove 1612 is an arc-shaped structure with a central angle of 0° to 360°, which allows the sample liquid to flow more smoothly to the pressure chamber 140. The recessed depth of the guide groove 1612 is 1 / 5 to 4 / 5 of the wall thickness of the assembly tube 168, which ensures that the liquid flows out smoothly, and the guide groove 1612 has a certain mechanical strength and is not easy to be damaged.

[0345] The radial liquid outlet groove 1611 is connected to the guide groove 1612 via a transition surface, and the transition surface is a vertical plane, an inclined surface or a curved surface.

[0346] The rear cell electrode 165 is embedded in the assembly seat 160 and is flush with, protruding from, or recessed from the outer end surface of the assembly seat 160 .

[0347] There is a first preset distance between the inner end of the radial liquid outlet groove 1611 and the inner end of the assembly tube 168; there is a second preset distance between the inner end of the rear cell electrode 165 and the inner end of the assembly tube 168; the first preset distance is less than or equal to the second preset distance.

[0348] like Fig.16 As shown in the figure, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 which are integrally connected. The rear pool cylinder 161 is provided with a radial liquid outlet groove 1611 and an axial drainage cavity 167. The end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163.

[0349] The outer cylinder 163 extends axially to form a plurality of positioning protrusions 1632 that are spaced apart from each other, and the buckling portions 1631 are disposed corresponding to the positioning protrusions 1632 .

[0350] The present application also provides a test kit 20, which includes a box body 100 and the aforementioned assembly seat 160, the box body 100 is provided with a front pool 120 and an installation cavity 130 that are connected; the assembly cylinder 168 of the assembly seat 160 is connected to the installation cavity 130, and the front pool 120 is connected to the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170.

[0351] The test kit 20 and its assembly seat 160 provided in this embodiment can facilitate two assembly seats 160 to share a pressure action chamber 140 connected to the two assembly seats 160, by arranging a radial liquid outlet groove 1611 on the assembly cylinder 168 and forming a recessed area on the outer surface of the radial liquid outlet groove 1611 to form a guide groove 1612, so as to facilitate negative pressure drainage of the pressure action chamber 140.

[0352] Please refer to the twenty-second embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides an assembly seat 160 , which includes an assembly tube 168 and a back cell electrode 165 .

[0353] The assembly tube 168 is a plastic assembly tube, which is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611 which are connected to each other. The radial liquid outlet groove 1611 is used to discharge the gas or liquid in the axial drainage chamber 167; the rear cell electrode 165 is connected to the assembly tube 168 and extends to the axial drainage chamber 167.

[0354] The outer surface of the assembly cylinder 168 is provided with a recessed area connected to the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 can make the gas or liquid in the axial drainage chamber 167 flow smoothly to the pressure chamber 140 through the guide groove 1612 (for example, obliquely above) after it comes out of the radial liquid outlet groove 1611 (for example, directly above). Thus, the two assembly seats 160 can share a pressure chamber 140 connected to the two assembly seats 160. The pressure chamber 140 passes through the through hole 133 (which can be Fig.17 The fan-shaped structure shown in FIG. 1 is connected to the installation cavity 130 and is further connected to the axial drainage cavity 167 through the guide groove 1612 and the radial liquid outlet groove 1611 in sequence.

[0355] The assembly cylinder 168 is provided with a buckling portion 1631, which is aligned with the radial liquid outlet groove 1611. The buckling portion 1631 can be a buckling hole. The buckling portion 1631 and the radial liquid outlet groove 1611 are aligned to facilitate demolding from the same direction during injection molding.

[0356] The inner end of the radial liquid outlet groove 1611 and the inner end of the assembly tube 168 are at a first preset distance; the inner end of the rear cell electrode 165 and the inner end of the assembly tube 168 are at a second preset distance; the first preset distance is less than or equal to the second preset distance. When the first preset distance is less than the second preset distance, the bubbles in the axial drainage cavity 167 are more easily discharged from the axial drainage cavity 167. If bubbles remain in the axial drainage cavity 167, the detection accuracy will be affected.

[0357] The axial length of the radial liquid outlet groove 1611 in the axial drainage cavity 167 is greater than or equal to the length of the rear cell electrode 165 extending toward the axial drainage cavity 167 .

[0358] The inner end of the assembly tube 168 is provided with a microporous sheet 170 that allows cells to pass through one by one. The distance from the inner end of the rear cell electrode 165 to the microporous sheet 170 is a third preset distance, and the third preset distance is 0.2-2 times the axial length of the axial drainage cavity 167. The inner end of the assembly tube 168 is provided with a sink 1613, and the microporous sheet 170 is connected to the sink 1613 in a cooperative manner.

[0359] In the embodiment of the present application, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 that are integrally connected. The rear pool cylinder 161 is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced apart and sleeved on the outer periphery of the rear pool cylinder 161. The buckling portion 1631 is provided on the outer cylinder 163.

[0360] The present application also provides a test kit 20, which includes a box body 100 and the aforementioned assembly seat 160, the box body 100 is provided with a front pool 120 and an installation cavity 130 that are connected to each other; the assembly cylinder 168 of the assembly seat 160 is connected to the installation cavity 130, the front pool 120 is connected to the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170, the front pool 120, the installation cavity 130, the axial drainage cavity 167, the radial liquid outlet groove 1611, and the pressure action cavity 140 together constitute a drainage channel, and the axial drainage cavity 167 can receive negative pressure to draw the sample to be tested in the front pool 120 through the micropores 172 of the microporous sheet 170.

[0361] The reagent kit 20 and its assembly seat 160 provided in this embodiment have novel structures and are easy to manufacture and assemble.

[0362] Please refer to the twenty-third embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a reagent kit 20 , which includes a box body 100 , a hemolytic agent pool 106 disposed on the box body 100 , and a diluent pool 111 disposed near the hemolytic agent pool 106 .

[0363] At least any two of the hemolytic agent pool 106, the diluent pool 111 and the box body 100 are integrally connected, and at least any two of the hemolytic agent pool 106, the diluent pool 111 and the box body 100 are detachably connected. In other words, the box body 100, the hemolytic agent pool 106 and the diluent pool 111 can be a conjoined structure or any separate structure.

[0364] For example, the box body 100, the hemolytic agent pool 106, and the diluent pool 111 are three independent parts and can be detachably connected to each other; or, the diluent pool 111 and the box body 100 are an integral structure, and a separate hemolytic agent container 206 is detachably assembled in the hemolytic agent pool 106; or, the hemolytic agent pool 106 and the box body 100 are an integral structure, and a separate diluent pool test tube is detachably assembled in the diluent pool 111; or, the hemolytic agent pool 106 and the diluent pool 111 are connected as a whole and are detachably connected to the box body 100.

[0365] Any two of the hemolytic agent pool 106, the diluent pool 111, and the box body 100 form a combined unit, and an assembly portion (e.g., the inner peripheral wall of the insertion hole provided on the box body 100) is provided on the combined unit, and a disassembly portion (e.g., the inner peripheral wall of the insertion hole provided on the box body 100) is provided on the other one of the hemolytic agent pool 106, the diluent pool 111, and the box body 100. Fig.15 The outer peripheral wall of the hemolytic agent container 206 shown in the figure), the disassembly portion is cooperatively connected with the assembly portion.

[0366] The surface of the assembly portion or the disassembly portion is further provided with a protruding structure 2061 , and the protruding structure 2061 can make the hemolytic agent container 206 abut and fit tightly with the insertion hole provided on the box body 100 .

[0367] In one embodiment, two assembly positions are provided on the box body 100 , and the hemolytic agent pool 106 and the diluent pool 111 are respectively installed in the two assembly positions.

[0368] In the embodiment of the present application, the box body 100 includes a front pool 120 and a mounting cavity 130 that are connected to each other. The front pool 120 and the mounting cavity 130 are connected through a through hole 132 (see Fig.17 and Fig.18 ) are connected, the front pool 120 is equipped with a front pool 120 electrode 121, the reagent kit 20 also includes an assembly seat 160 connected to the installation cavity 130, the assembly seat 160 is provided with a microporous sheet 170 and a rear pool electrode 165, the microporous sheet 170 is installed at the through hole 132, the front pool 120 electrode 121 and the rear pool electrode 165 are respectively spaced apart on both sides of the microporous sheet 170, the assembly seat 160 includes an assembly cylinder 168, the assembly cylinder 168 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611.

[0369] In the embodiment of the present application, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 that are integrally connected. The rear pool cylinder 161 is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced apart and sleeved on the outer periphery of the rear pool cylinder 161.

[0370] The usage of the diluent pool 111 is generally relatively large, so in the embodiment of the present application, the volume of the diluent pool 111 is greater than or equal to the volume of the hemolytic agent pool 106 .

[0371] The open ends of the diluent reservoir 111 and the hemolytic agent reservoir 106 are provided with sealing films to facilitate long-term storage of the diluent and hemolytic agent.

[0372] The reagent kit 20 provided in this embodiment has a novel structure and multiple detachable solutions, and can adapt to a variety of different detection requirements.

[0373] Please refer to the twenty-fourth embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a test kit 20, which includes a box body 100 and at least one item detection pool, and the at least one item detection pool is integrally connected to the box body 100 or detachably connected.

[0374] The item detection pool includes a first item detection pool for performing a first item detection and a second item detection pool for performing a second item detection. The first item detection pool and / or the second item detection pool are integrally connected to the box body 100 or detachably connected.

[0375] The test kit 20 further includes a third item detection pool for performing a third item detection, and the third item detection pool is integrally connected to the box body 100 or detachably connected thereto.

[0376] The second project detection pool and the third project detection pool are arranged on the same side of the first project detection pool; or the second project detection pool and the third project detection pool are arranged on both sides of the first project detection pool respectively.

[0377] The detection items of the first item detection pool, the second item detection pool, and the third item detection pool can be selected from specific protein detection, biochemical detection, immunological detection, and routine blood test. For example, routine blood test can be performed by cooperating with the front pool 120, the installation cavity 130, the assembly seat 160, and the pressure action cavity 140, and specific protein detection, biochemical detection, immunological detection, etc. can be performed by cooperating with the optical detection cup.

[0378] The detection items of the first item detection pool, the second item detection pool, and the third item detection pool are the same, different, or not completely the same; or any one of the first item detection pool, the second item detection pool, and the third item detection pool supports more than two detection items.

[0379] The box body 100 is provided with an insertion hole, which may include a rectangular insertion hole 107 and a circular insertion hole 108. The second item detection pool is inserted through the insertion hole, or the second item detection pool is provided with a flange to cooperate with the insertion hole.

[0380] The second item detection cell includes a first cell body 207, a second cell body 208, and a connector 2071 connecting the first cell body 207 and the second cell body 208. The connector 2071 connects the first cell body 207 and the second cell body 208 to the box body 100 in the form of an assembly.

[0381] The first item detection pool and / or the second item detection pool are light-transmitting plastic pools or glass pools. The opening ends of the first item detection pool and / or the second item detection pool are provided with sealing films, which can be pierced by the piercing head 204 provided on the box body 100 for use.

[0382] The reagent kit 20 provided in this embodiment has a novel structure and multiple detachable solutions, and can adapt to a variety of different detection requirements.

[0383] Please refer to the twenty-fifth embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a test kit 20, which includes a box body 100. The box body 100 includes a front pool 120, an installation cavity 130 and a pressure chamber 140. The front pool 120 is connected to the installation cavity 130, and the pressure chamber 140 is connected to the installation cavity 130. The pressure chamber 140 is vertically arranged, and the installation cavity 130 is horizontally arranged.

[0384] The opening end of the installation cavity 130 faces the outer surface of the box body 100 . The reagent kit 20 further includes an assembly seat 160 , which is connected to the installation cavity 130 . The assembly seat 160 is provided with an axial drainage cavity 167 , which is connected to the pressure action cavity 140 .

[0385] A drainage gap is formed between the installation cavity 130 and the assembly seat 160 , and the axial drainage cavity 167 is connected with the pressure action cavity 140 through the drainage gap.

[0386] The assembly seat 160 is provided with a radial liquid outlet groove 1611, and the axial drainage chamber 167 is connected to the pressure action chamber 140 through the radial liquid outlet groove 1611; the assembly seat 160 is provided with a guide groove 1612 adjacent to the radial liquid outlet groove 1611, and the axial drainage chamber 167 is connected to the pressure action chamber 140 through the radial liquid outlet groove 1611 and the guide groove 1612.

[0387] The assembly seat 160 is provided with a microporous sheet 170 and a rear cell electrode 165 , and the front cell 120 is provided with a front cell 120 electrode 121 . The front cell 120 electrode 121 and the rear cell electrode 165 are respectively spaced apart and located on both sides of the microporous sheet 170 .

[0388] The assembly seat 160 and the installation cavity 130 are snap-fitted, threaded, interference-fitted, laser-welded, or adhesively-fitted.

[0389] The reagent kit 20 further includes an inner sealing ring 164 and an outer sealing ring 166 . The inner sealing ring 164 is disposed between the microporous sheet 170 and the box body 100 , and the outer sealing ring 166 is disposed between the assembly seat 160 and the box body 100 .

[0390] In the embodiment of the present application, the front pool 120 and the installation cavity 130 are both in two groups, and one pressure action cavity 140 is connected to the two groups of installation cavities 130 .

[0391] The test kit 20 provided in this embodiment has a novel structure, wherein the front pool 120, the installation cavity 130, and the pressure action cavity 140 are interconnected and arranged vertically in sequence. The opening end of the pressure action cavity 140 is located on the upper surface of the box body 100, and the negative pressure source can be applied from above the box body 100, thereby simplifying the structure of the box body 100 and avoiding the side of the box body 100 having more protruding structures.

[0392] Please refer to the twenty-sixth embodiment. Fig.15 and Fig. 20 An embodiment of the present application provides a reagent kit 20, which includes a plurality of first pool positions, and the centers of the plurality of first pool positions are arranged roughly in a first straight line or a first arc.

[0393] The reagent kit 20 also includes a plurality of second pools arranged approximately in a second straight line at the center, and the first straight line and the second straight line are arranged in parallel or vertically spaced relation.

[0394] Alternatively, the reagent kit 20 includes a plurality of second pool positions arranged in a second arc at the center, the first arc and the second arc are arranged in parallel and spaced apart, and the arc can be an arc corresponding to a central angle of 0 to 360 degrees.

[0395] The plurality of first cell sites and / or the plurality of second cell sites include impedance detection cells and / or optical detection cells.

[0396] The multiple first pool positions and / or the multiple second pool positions also include a diluent pool 111, which is used to encapsulate the diluent; the multiple first pool positions and / or the multiple second pool positions also include a hemolytic agent pool 106, which is used to encapsulate the hemolytic agent; or the multiple first pool positions and / or the multiple second pool positions also include a sample dilution pool 112, which is used for sample dilution.

[0397] The impedance detection pool is provided with a light-transmitting detection window for cooperating with optical detection, that is, one of the front pools 120 is provided with a light-transmitting detection window for cooperating with optical detection. The front pool 120 can be made of transparent plastic as a whole, and the transmittance and smoothness of the light-transmitting detection window can be the same as those of the front pool 120 or higher than other parts of the front pool 120.

[0398] The plurality of first pool positions and / or the plurality of second pool positions further include a plurality of insertion holes.

[0399] The plurality of first pool positions and / or the plurality of second pool positions include at least one tip head holding pool (101, 102, 103) and / or a sample holding pool 105.

[0400] The plurality of first pool positions and / or the plurality of second pool positions include at least one puncture head accommodating pool 104. The reagent kit 20 provided in the present application is a degradable plastic reagent kit 20, and by arranging the plurality of pool positions in a straight line, it is convenient for the pipetting device to move a shorter path during automated testing, wherein the pipetting device is used to transfer and mix the liquids in each pool body.

[0401] Please refer to the twenty-seventh embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a test kit 20, which includes a box body 100, on which are provided a detection area (such as the area formed by the front pool 120, the installation cavity 130, the pressure action cavity 140, and the assembly seat 160), a reagent area (such as the area formed by the diluent pool 111, the hemolytic agent pool 106, and the sample holding pool 105), and an accessory placement area (such as the area formed by the tip head holding pools 101, 102, 103 and the puncture head holding pool 104).

[0402] The box body 100 also includes an expandable area (such as the area formed by the rectangular insertion hole 107 and the circular insertion hole 108 in the figure), which is arranged close to the detection area, located on one side of the detection area, or located on one side of the reagent area.

[0403] The accessory placement area is arranged close to the reagent area, and the detection area is arranged close to the reagent area.

[0404] The detection area includes an impedance detection cell and / or an optical detection cell, and the optical detection cell is used to detect any one of the parameters of HGB, CRP, and SAA.

[0405] The reagent area includes at least one of a diluent pool, a hemolytic agent pool, and a sample holding pool 105 .

[0406] The reagent area includes a cell body area, and the cell body area is used to detachably mount at least one of a diluent container, a hemolytic agent container 206 and a sample containing cell 105 .

[0407] The accessory placement area includes placement positions for tip heads 201 , 202 , 203 and / or puncture head 204 .

[0408] The expandable area includes a detachable optical detection cell and / or an impedance detection cell, and the optical detection cell is used to detect any one of the parameters of HGB, CRP, and SAA.

[0409] The test kit 20 provided in the present application is a degradable plastic test kit 20, which can facilitate the movement of the pipetting device over a shorter path during automatic detection through a partitioned setting.

[0410] Please refer to the twenty-eighth embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a reagent kit 20, which includes a box body 100, on which a detection area (such as the area formed by the front pool 120, the installation cavity 130, the pressure action cavity 140, and the assembly seat 160), a reagent area (such as the area formed by the diluent pool 111, the hemolytic agent pool 106, and the sample holding pool 105) and an accessory placement area (such as the area formed by the tip head holding pools 101, 102, 103 and the puncture head holding pool 104) are arranged, and the reagent area is arranged close to the accessory placement area.

[0411] The box body 100 also includes an expandable area (such as the area formed by the rectangular insertion hole 107 and the circular insertion hole 108 in the figure), which is arranged close to the detection area. Alternatively, the expandable area is arranged close to the side of the detection area or close to the side of the reagent area.

[0412] The reagent area includes a plurality of reagent cells, and the plurality of reagent cells are arranged roughly in a straight line or in an arc.

[0413] The detection area includes an impedance detection cell and / or an optical detection cell, and the optical detection cell is used to detect any one of the parameters of HGB, CRP, and SAA.

[0414] The accessory placement area includes placement positions for tip heads 201 , 202 , 203 and / or puncture head 204 .

[0415] The expandable area includes a detachable optical detection cell and / or an impedance detection cell, and the optical detection cell is used to detect any one of the parameters of HGB, CRP, and SAA.

[0416] The reagent area includes at least two cell body areas, and at least two cell body areas include bottomed pools and / or bottomless insertion holes.

[0417] The accessory area includes one or more bottomed pools for mounting tip heads 201 , 202 , 203 and / or piercing head 204 .

[0418] The expandable area is provided with a circular insertion hole 108 and a rectangular insertion hole 107, and the circular insertion hole 108 and the rectangular insertion hole 107 are used to install a detection pool, which is an optical detection pool and / or an impedance detection pool. The reagent kit 20 provided in the present application is a degradable plastic reagent kit 20, and the partitioned setting can facilitate the movement of the pipette device for a shorter path during automatic detection.

[0419] Please refer to the twenty-ninth embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a test kit 20, which includes a box body 100. The test kit 20 is used for sample detection. The box body 100 is provided with a supporting portion so that the test kit 20 can be stably supported on a placing surface.

[0420] In one embodiment, the box body 100 includes two support plates (not shown), which serve as support parts. The support plates may be provided with a mark, which may be a barcode, a QR code or an identification chip to record relevant parameters of the reagent kit 20.

[0421] At least one supporting platform or supporting surface is provided at the box body 100 near the center of gravity of the reagent box 20, and the supporting platform or supporting surface serves as a supporting portion.

[0422] In one embodiment, the box body 100 is provided with three supporting protrusions, and the three supporting protrusions form a triangle, and the projection of the center of gravity of the reagent box 20 falls within the area surrounded by the triangle.

[0423] In one embodiment, the box body 100 includes at least one pool body, and the bottom of the pool body can be used as a support portion.

[0424] Optionally, the supporting plate or the supporting platform or the supporting surface or the supporting protrusion and the bottom of the pool body serve together as a supporting portion.

[0425] The pool body includes a receiving chamber and a skirt 124 extending downward from the receiving chamber (see Fig.18 , the structure below the bottom 123 of the front pool 120), the skirt 124 serves as a support portion, and the accommodating cavity can be used to hold reagents, samples, or accessories (201-204).

[0426] The box body 100 provided in the present application is a degradable plastic box body. The reagent box 20 is provided with an impedance detection cell and / or an optical detection cell.

[0427] The reagent kit 20 provided in this embodiment is provided with more pools, and a stable support may be formed through the bottom of the pools and / or additionally provided support parts to prevent the reagent kit 20 from tipping over easily.

[0428] Please refer to the 30th embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a test kit 20, which includes a box body 100. The box body 100 includes a plurality of pool bodies. The pool body includes a receiving cavity and a support portion connected to the receiving cavity. The support portion is a solid structure or a hollow structure. The provision of a solid or hollow support portion can increase the relative height of the bottom of the receiving cavity, which can facilitate the absorption of reagents. The corresponding pipetting device does not need to be inserted too deeply, thereby saving the pre-stored amount of reagents. If it is a solid structure, the center of gravity of the box body 100 can also be moved down, making the overall structure more stable; when it is hollow, the plastic material of the box body 100 can be saved.

[0429] The height of the supporting portion is greater than or equal to the wall thickness of the accommodating cavity. The bottom of the accommodating cavity may be a conical bottom, specifically a conical arc bottom, a triangular pyramid bottom or a polygonal pyramid bottom, and the conical bottom extends into the hollow area at the bottom of the supporting portion; or the bottom of the accommodating cavity is a plane bottom, and the accommodating cavity is connected to the supporting portion through the plane bottom.

[0430] When the supporting part is a hollow structure, an open end or a closed end is provided at the bottom of the supporting part.

[0431] The accommodating cavity and the supporting part are integrally formed or detachably connected.

[0432] When the support part is a hollow structure, the cross section of the hollow structure is square, circular, polygonal or irregular, and the height of the support part is 0.1 to 0.8 times the height of the pool body.

[0433] The aforementioned cell body may specifically be any cell body such as a reagent cell, a detection cell, a sample dilution cell 112 or an accessory placement cell (101-104).

[0434] The aforementioned detection cell is used for impedance detection or optical detection.

[0435] An embodiment of the present application also provides a sample detection device, which includes the aforementioned test kit 20 and a detection seat 300 that cooperates with the test kit 20. The detection seat 300 is used for sample analysis and detection. The detection seat 300 is provided with a power supply component that is electrically connected to the front cell 120 electrode 121 and the rear cell electrode 165. The detection seat 300 may also be provided with optical detection components located on opposite sides of the optical detection cell (for example, the first cell body 207).

[0436] The reagent kit 20 provided in this embodiment can facilitate liquid transfer by a liquid transfer device by setting pool bottoms of different heights. The liquid transfer device does not need to be inserted too deep, thereby saving the pre-stored amount of reagents.

[0437] Please refer to the thirty-first embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a test kit 20, which includes a box body 100. The box body 100 is provided with an accessory placement area (101-104), and the accessory placement area is used to place accessories (201-204).

[0438] The lower side of the accessory placement area is provided with a liquid-leakage-proof structure, which can be a bottom pool. The liquid-leakage-proof structure is integrally formed with the box body 100 or is detachably connected. The accessory placement area is provided with a placement hole, and the accessories (201-204) are placed in the placement hole.

[0439] The aperture of the placement hole is smaller than or equal to the maximum radial dimension of the accessory (201-204), so as to prevent the accessory (201-204) from being completely sunk into the placement hole and to prevent the bottom of the accessory (201-204) from contacting and deforming with the bottom of the pool body when the accessory (201-204) is plugged in.

[0440] The box body 100 is provided with a receiving cavity corresponding to the placement hole, and the accessories (201-204) are received in the receiving cavity. The bottom of the receiving cavity is a sealing structure.

[0441] The accommodating cavity and the box body 100 are integrally formed or detachably connected.

[0442] The inner cavity depth of the accommodating cavity is greater than or equal to the length of the accessory (201-204) extending into the accommodating cavity, so as to prevent the accessory (201-204) from protruding too much from the surface of the box body 100 when placed in the accommodating cavity, and at the same time, to prevent the bottom of the accessory (201-204) from contacting and deforming with the bottom of the pool body when the accessory (201-204) is plugged in.

[0443] The length of the attachment (201-204) extending into the accommodating cavity is 0.4 to 1 times the length of the attachment.

[0444] The embodiment of the present application further provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20, and the detection seat 300 is used for sample analysis and detection.

[0445] When the accessories of the reagent kit 20 provided in this embodiment are removed, the accessories can be received by the bottom pool to prevent the residual liquid carried on the surface of the accessories from leaking.

[0446] Please refer to the thirty-second embodiment. Fig.15 and Fig. 20 An embodiment of the present application provides a detection cup assembly, which includes a first cell body 207 and a second cell body 208 connected to the first cell body 207 .

[0447] The first cell body 207 is connected to the second cell body 208 via a connecting piece 2071 , and the connecting piece 2071 is integrally formed with at least one of the first cell body 207 and the second cell body 208 or is detachably connected thereto.

[0448] The connecting member 2071 is provided with at least one cup holder 2072 , and the cup holder 2072 is detachably connected to the first cell body 207 or the second cell body 208 .

[0449] In one embodiment, the connecting member 2071 is provided with a cup holder 2072 and is integrally connected to the first tank body 207 . The cup holder 2072 is provided with a placement hole, and the shape of the placement hole is the same as or different from the cross-sectional shape of the first tank body 207 .

[0450] In one embodiment, the connecting member 2071 is provided with two cup holders 2072 , and the two cup holders 2072 are respectively provided with a first placement hole and a second placement hole, and the shapes of the first placement hole and the second placement hole are the same or different.

[0451] The first cell body 207 is a detection cup, and the second cell body 208 is a reagent cup. During detection, the reagent in the second cell body 208 is added to the first cell body 207 to prepare the sample to be detected and then the detection is performed.

[0452] An optical detection window is provided on the first cell body 207 for optical detection, such as optical detection by transmitted light or optical detection by scattered light.

[0453] The cross-sectional shapes of the first cell body 207 and the second cell body 208 are the same or different; or the heights of the first cell body 207 and the second cell body 208 are the same or different. The differentiated settings can achieve a fool-proof effect and facilitate assembly and identification.

[0454] The embodiment of the present application further provides a test kit 20, which includes a box body 100 and the aforementioned detection cup assembly, the box body 100 is provided with a mounting portion, the detection cup assembly is provided with a matching portion, and the mounting portion is connected to the matching portion.

[0455] The mating part may be a raised part or a recessed part provided on the side surface of the detection cup assembly, which is matingly connected with the mounting part; or the mating part may be a positioning flange provided on the detection cup assembly, which is matingly connected with the mounting part, for example, by using a flange structure to be hung with the insertion hole on the box body 100.

[0456] The detection cup assembly provided in this embodiment has a novel structure. Specific detection items can be equipped with corresponding required reagents on the cup holder 2072, which can greatly improve the detection efficiency and allow flexible selection of detection items.

[0457] Please refer to the thirty-third embodiment. Figures 15 to 20 An embodiment of the present application provides a reagent kit 20 , which includes a box body 100 and an assembly seat 160 .

[0458] The box body 100 includes a front cell 120, which is equipped with a front cell 120 electrode 121; an assembly seat 160 is connected to the box body 100, and the assembly seat 160 is equipped with a rear cell electrode 165, and the front cell 120 electrode 121 and the rear cell electrode 165 are arranged at intervals.

[0459] In the embodiment of the present application, the axis of the front cell 120 electrode 121 and the axis of the rear cell electrode 165 are roughly in the same straight line. Experimental verification shows that when the axis of the front cell 120 electrode 121 and the rear cell electrode 165 are coaxial, the detection accuracy is relatively high. The front cell 120 electrode 121 and the rear cell electrode 165 are both columnar electrodes, and the manufacturing process and assembly process are relatively simple.

[0460] The assembly seat 160 is provided with an axial drainage cavity 167 , which is connected to the front cell 120 via a microporous sheet 170 . The front cell 120 electrode 121 and the rear cell electrode 165 are located on both sides of the microporous sheet 170 .

[0461] The microporous sheet 170 includes a sheet body 171 , which is provided with micropores 172 that allow cells to pass through one by one. The microporous sheet 170 is mounted on the assembly seat 160 or the box body 100 , or the microporous sheet 170 and the assembly seat 160 or the box body 100 are an integrally formed structure.

[0462] The axis of the electrode 121 of the front cell 120 , the axis of the rear cell electrode 165 , and the axis of the microporous sheet 170 are substantially in the same straight line.

[0463] The front cell 120 electrode 121 protrudes, is flush with, or is recessed in the inner wall of the front cell 120; the rear cell electrode 165 protrudes, is flush with, or is recessed in the bottom wall of the axial drainage cavity 167. The two ends of the front cell 120 electrode 121 and the rear cell electrode 165 are not particularly limited. The front cell 120 electrode 121 and the rear cell electrode 165 are in contact with the liquid to be tested during detection, and the outer ends of the front cell 120 electrode 121 and the rear cell electrode 165 are used for external power supply components.

[0464] The box body 100 is provided with a mounting cavity 130 , and the assembly seat 160 includes an assembly tube 168 , and the mounting cavity 130 is coaxially connected with the assembly tube 168 .

[0465] The assembly cylinder 168 and the installation cavity 130 are snap-fitted, threaded, interference-fitted, laser-welded, or adhesively-fitted.

[0466] The front cell 120 electrode 121 is disposed on the box body 100 and protrudes, is flush with, or is recessed on the outer side of the box body 100 . The rear cell electrode 165 is disposed on the assembly seat 160 and protrudes, is flush with, or is recessed on the outer side of the assembly seat 160 .

[0467] The embodiment of the present application further provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20, and the detection seat 300 is used for sample analysis and detection.

[0468] Please refer to the thirty-fourth embodiment. Fig.15 and Fig. 20 , an embodiment of the present application provides a reagent kit 20 , the reagent kit 20 includes a box body 100 and a gripping portion 150 , and the gripping portion 150 is disposed on the box body 100 .

[0469] The gripping portion 150 may be disposed near the center of the side edge of the box body 100 , or near the center of the upper surface of the box body 100 .

[0470] There are two gripping portions 150 , which are disposed on two opposite sides of the box body 100 .

[0471] The gripping portion 150 protrudes from the upper surface or the side surface of the box body 100. The outer surface of the gripping portion 150 is provided with a buckle protrusion 151 or a buckle recess.

[0472] An anti-slip portion 152 is disposed on the top or outer side of the grip portion 150 .

[0473] The gripping portion 150 is an elastic member, and a recessed portion 154 is provided on the side of the box body 100 . The gripping portion 150 is connected with the recessed portion 154 in a cooperative manner.

[0474] The gripping portion 150 and the recessed portion 154 are connected integrally or detachably.

[0475] The gripping portion 150 is slidably connected to the recessed portion 154 so that the gripping portion 150 and the box body 100 can move relative to each other. The gripping portion 150 can be extended and retracted relative to the box body 100, which is convenient for storage and does not occupy space. The gripping portion 150 can be pulled out when needed.

[0476] The embodiment of the present application further provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20 , and the gripping portion 150 is connected to the detection seat 300 in a cooperative manner.

[0477] Please refer to the thirty-fifth embodiment. Fig.15 and Fig. 20 The embodiment of the present application provides a reagent kit 20 , which includes a box body 100 and a blocking portion 153 .

[0478] The box body 100 includes at least one pool position (eg, a sample containing pool 105 ); the pool position is used to place a test tube (eg, a sample tube 205 ).

[0479] The blocking portion 153 is disposed at one side of the pool, and is used to hook the test tube cap (not shown) of the test tube to prevent the opened test tube cap from returning to cover the open end of the test tube.

[0480] In a specific embodiment, the blocking portion 153 may be protruding, flush, or recessed on the upper surface or side surface of the box body 100 .

[0481] In one embodiment, the blocking portion 153 includes a connecting member connected to the box body 100 and a stopper portion extending from the connecting member. A hook portion extending toward the box body 100 is disposed at the end of the stopper portion.

[0482] In another embodiment, the side surface of the box body 100 protrudes outward to form the blocking portion 153; or the blocking portion 153 is a groove set on the upper surface of the box body 100; or the upper surface of the box body 100 is provided with a recessed portion, and the inner wall of the recessed portion forms the blocking portion 153, and the test tube cap of the test tube can be inserted into the recessed portion after being opened, and the size of the recessed portion can correspond to the size of the test tube cap to achieve a relatively tight fit.

[0483] The blocking portion 153 is integrally formed or detachably connected with the box body 100. Specifically, the blocking portion 153 is snap-fitted, plug-fitted, threadedly connected with the box body 100, or detachably connected with the box body 100 via screws / pins.

[0484] In other embodiments, the blocking portion 153 is also slidably connected to the box body 100 .

[0485] In the embodiment of the present application, the pool position (eg, the sample containing pool 105 ) is disposed close to the edge of the box body 100 , and the blocking portion 153 is disposed at the edge of the box body 100 .

[0486] The above are only implementation methods of the present application, and are not intended to limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A POCT blood cell analyzer, characterized in that: The POCT blood cell analyzer comprises: A detection seat, used to install a test kit, wherein the test kit includes an impedance detection cell for performing impedance detection, wherein the impedance detection cell includes a front cell and a rear cell connected by micropores; A pressure building component is connected to the gas path of the front pool and / or the rear pool, and is used to provide pressure so that the liquid to be tested in the front pool flows to the rear pool through the micropores, and is also used to provide pressure to the front pool or the rear pool before the liquid to be tested is injected into the front pool so that the micropores are unobstructed; A pipette connected to the air path of the pressure building component, the pipette is arranged above the detection seat and is used to perform corresponding operations on the reagent kit; The pressure building component includes a pressure chamber, an air pressure connection device, a first syringe, a second syringe and a driving member, the first syringe is at least connected to the pressure chamber to establish positive pressure or negative pressure in the pressure chamber, the pressure chamber is connected to the air pressure connection device, the second syringe is connected to the pipette to suck out samples and / or reagents, the driving member is used to drive the first syringe and the second syringe at the same time, the first syringe and the second syringe are linked syringes, The POCT blood cell analyzer also includes a first solenoid valve, a second solenoid valve, and a third solenoid valve. The first solenoid valve is used to selectively connect the first syringe with the pressure chamber and the second syringe; the second solenoid valve is used to selectively connect the first solenoid valve with the pressure chamber and the outside atmosphere; the third solenoid valve is used to selectively connect the air pressure connection device with the pressure chamber.

2. The POCT blood cell analyzer according to claim 1, characterized in that: The pressure building component is also used to mix the liquid to be tested.

3. The POCT blood cell analyzer according to claim 1, characterized in that: The POCT blood cell analyzer comprises a plurality of groups of gate valves, and the gate valves are connected to the pressure chamber and the syringe.

4. The POCT blood cell analyzer according to claim 3, characterized in that: The POCT blood cell analyzer includes a metal shielding cover matched with the detection seat, and the metal shielding cover is provided with a pipeline connected to the pressure chamber or the syringe, and the pipeline is used to communicate with the rear pool or the front pool.

5. The POCT blood cell analyzer according to claim 4, characterized in that: The metal shielding cover is configured to move in a vertical direction.

6. The POCT blood cell analyzer according to claim 4, characterized in that: The metal shielding cover is configured to rotate in a horizontal direction and reciprocate in a vertical direction.

7. A method for using a POCT blood cell analyzer, characterized in that: Based on the POCT blood cell analyzer according to any one of claims 1 to 6, wherein the method of use comprises: The receiving reagent kit is loaded into the detection seat, wherein the reagent kit includes an impedance detection cell for performing impedance detection, and the impedance detection cell includes a front cell and a rear cell connected by micropores; Before the test liquid is injected into the front cell, pressure is provided to the front cell or the back cell to make the micropores unobstructed.

8. The method of use according to claim 7, characterized in that: The step of providing pressure to the front pool or the back pool to make the micropores unobstructed before the test liquid is injected into the front pool comprises: Providing pressure to the front pool or the rear pool by the pressure in the pressure chamber of the pressure building component so that the micropores are unblocked; or The syringe of the pressure building component provides pressure to the front pool or the rear pool to make the micropores unblocked.

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