A device for detecting carbonyl mycotoxins in food

Abstract

The present invention discloses a device for detecting carbonyl mycotoxins in food in the field of food detection, comprising an external detection device, the detection device comprising a photodetector and a biosensor, and also comprising a built-in refining device, the refining device comprising a high-pressure pump and a tank body connected to each other, the top of the tank body being provided with a tank cover, the interior of the tank body being provided with a coarse filter component and a fine filter component in sequence from top to bottom; the coarse filter component comprising a first filter disc and a first filter membrane, the fine filter component comprising a membrane cover, a second filter disc and a second filter membrane, the surfaces of the first filter disc and the second filter disc being provided with a plurality of vertical grids, parallel flow channels being formed between adjacent grids, and stratified treatment of oil and fat and alcohol substances being performed, so as to facilitate improvement of detection targeting and sensitivity, and at the same time, due to the arrangement of the first filter membrane and the second filter membrane, a membrane wrapping structure is formed, so as to facilitate subsequent colony culture (especially membrane wrapping culture method) and substance detection.

Description

Technical Field

[0001] The invention belongs to the field of food detection, and in particular relates to a device for detecting carbonyl mycotoxins in food. Background Art

[0002] "Food is the first necessity of the people, and food safety is the first priority." With the continuous improvement of living standards, people's requirements for food are much stricter than before. It is not limited to being able to eat enough. How to eat well and eat nutritiously has become the main direction of today's life. Everyone's attention is gradually turning to food. With the rapid development of food production, processing and circulation in my country, many unscrupulous vendors have begun to take shortcuts, so food safety has become a major issue of concern to everyone.

[0003] As the number of diseases caused by pathogenic bacteria in microorganisms continues to increase, and pathogenic bacteria in food can endanger people's health, the incidents of microbial contamination of food and human health announced abroad still exist in my country. Therefore, the determination of total colony count, mold, and pathogenic bacteria in food is an important indicator to judge whether the product quality is qualified and whether it poses a hazard to human health.

[0004] In order to achieve the above-mentioned purpose, the prior art CN207036694U discloses a food fluorescence detection device. This detection device adds a fixing base on the basis of the existing food fluorescence detection device. The main body of the fluorescence detector is fixed on the operating table through the suction cup at the bottom of the fixing base, which can reduce the possibility of the main body of the fluorescence detector falling due to collision.

[0005] However, the fluorescence detector of the above scheme can only detect separated substances. For some oily or alcoholic liquid foods, the above scheme lacks colony culture equipment and cannot provide good detection results. At the same time, for oily or alcoholic foods, carbonyl is an important evaluation indicator. The above fluorescence detector cannot detect liquid foods after staining, and the detection range is not comprehensive. Summary of the invention

[0006] In order to solve the above problems, the purpose of the present invention is to provide a carbonyl mycotoxin detection device that is convenient for realizing the diversion of liquid food.

[0007] In order to achieve the above-mentioned object, the technical scheme of the present invention is as follows: a device for detecting carbonyl mycotoxins in food, comprising an external detection device, the detection device comprising a photon detector and a biosensor, and a built-in refining device, the refining device comprising a high-pressure pump and a tank body connected to each other, the top of the tank body is provided with a tank cover, and the inside of the tank body is provided with a coarse filter component and a fine filter component in sequence from top to bottom;

[0008] The coarse filter component includes a first filter disc and a first filter membrane, and the fine filter component includes a membrane cover, a second filter disc and a second filter membrane. The surfaces of the first filter disc and the second filter disc are provided with a plurality of vertical grids, and parallel flow channels are formed between adjacent grids. The cross-sectional area of ​​the parallel flow channels is the same as the cross-sectional area of ​​the flow channels connected to the high-pressure pump. The surfaces of the parallel flow channels are provided with filter holes. The first filter membrane is bonded to the first filter disc of the coarse filter component, the membrane cover and the second filter disc are buckled together, and the position of the membrane cover facing the second filter disc is provided with spacer bars, and the spacer bars are grouped in pairs, and each group of spacer bars is directly opposite to the coverage range of the parallel flow channels, and a pressure flow channel with a gap is formed between the spacer bars and the parallel flow channels.

[0009] The following beneficial effects are achieved after adopting the above scheme: 1. When using this technical scheme, the alcohol and oil foods to be tested are first placed in the refining device, and the refining device is closed at this time, and then high pressure is continuously introduced. Since the cross-sectional area of ​​the parallel flow channel of the first filter disk is the same as the cross-sectional area of ​​the flow channel connected to the high-pressure pump, the food to be tested flows along the surface of the first filter disk under high pressure. At this time, the pressure gradually causes the food to be tested to flow along the parallel flow channel, and then the food to be tested is gradually pressed down through the filter holes by the pressure, and then the macromolecular substances in the food to be tested are retained by the first filter membrane, thereby completing the first screening. At this time, the macromolecular substances remain on the surface of the first filter disk.

[0010] 2. After the first screening is completed, the food to be tested passes through the membrane cover and enters the surface of the second filter disc. At this time, the spacer divides the parallel flow channels into a finer part of the flow channels. At this time, the parallel flow channels and the spacer are closed to each other, so that the food to be tested flowing here forms an extruded and closed space, thereby causing the food to be tested to gradually leak out under pressure, increasing the role and effect of extrusion. When the food to be tested passes through the second filter disc, the remaining strain components on the surface of the second filter membrane, and the remaining water and residues are at the bottom of the tank.

[0011] 3. This technical solution performs stratified treatment on oil and alcohol substances, which is convenient for improving the detection target and sensitivity. At the same time, due to the setting of the first filter membrane and the second filter membrane, it is convenient to form a membrane-wrapped structure, which is convenient for subsequent colony culture (especially membrane-wrapped culture method) and substance detection.

[0012] Furthermore, a carrying space is formed between the fine filtration component and the bottom of the refining device, a metering component is provided on one side of the width of the carrying space, the metering component includes a metal tube and a prompt circuit, the top of the metal tube is provided with opposing metal guide plates, the metal guide plates are connected to the prompt circuit, and a disconnection space is formed between the metal guide plates, a conductive block is slidably connected in the metal tube, the width of the conductive block is equal to the width of the disconnection space, and the movement stroke of the conductive block passes through the disconnection space.

[0013] Beneficial effect: When this technical solution is used, the gradually accumulated liquid in the carrying space forms the working conditions of the metering group. Therefore, when the liquid gradually accumulates, the liquid enters the metal tube. At this time, the liquid continuously piles up the conductive block in the metal tube. When the conductive block contacts the metal guide plate, the prompt circuit is connected. After being connected, the prompt circuit starts to conduct electricity. Therefore, the carried liquid is measured, which is convenient for monitoring the accumulation and filtration of the liquid in a closed high-pressure environment, so as to release the high pressure in time and extend the service life of the device. At the same time, the use of glass materials is avoided to prevent the glass material from bursting under pressure.

[0014] Furthermore, the prompt circuit includes a power supply and a lamp bead, and when the conductive block moves to the disconnected space, a closed loop is formed in the prompt circuit.

[0015] Beneficial effect: This technical solution uses the opening and closing conditions of external lamp beads to detect the filling conditions of the solution.

[0016] Furthermore, the cross section of the conductive block is trapezoidal, the inside of the conductive block is hollow, and the outside of the conductive block is made of copper.

[0017] Beneficial effect: This technical solution increases the contact area between the conductive block and the liquid surface, thereby allowing the conductive block to float on the liquid surface.

[0018] Furthermore, a vertical slide rail is built into the metal tube, and the slide rail is slidably connected to the conductive block.

[0019] Beneficial effect: the sliding mode of the conductive block is restricted, and at the same time, it is convenient for the conductive block to slide down along the slide rail after the liquid disappears.

[0020] Furthermore, an upper space is formed from the coarse filter assembly to the top of the tank body, a middle space is formed between the coarse filter assembly and the fine filter assembly, and the tank cover, the upper space, the middle space and the bearing space are threadedly connected.

[0021] Beneficial effect: The detachable connection is adopted, so the partial removal and docking can be realized.

[0022] Furthermore, the top of the tank cover is provided with an injection connection pipe and a pressure connection pipe.

[0023] Furthermore, a penetration hole is provided on the top of the film-closing cover.

[0024] Beneficial effect: The hole facilitates the inflow of food to be tested.

[0025] Furthermore, the mesh number of the first filter membrane is greater than the mesh number of the second filter membrane. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a schematic diagram of an embodiment of the present invention;

[0027] Figure 2 for Figure 1 A structural diagram of the first filter disc;

[0028] Figure 3 for Figure 1 Structural diagram of the second filter disc and the combined membrane cover. DETAILED DESCRIPTION

[0029] The following is further described in detail through specific implementation methods:

[0030] The figure marks in the drawings of the specification include: tank cover 1, injection connecting pipe 2, pressure connecting pipe 3, upper space 4, middle space 5, carrying space 6, first filter disc 7, first filter membrane 8, membrane cover 9, second filter disc 10, second filter membrane 11, through hole 12, grid 13, parallel flow channel 14, spacer bar 15, pressure flow channel 16, lamp bead 17, metal tube 18, metal guide plate 19, conductive block 20, slide rail 21.

[0031] Embodiment 1

[0032] The embodiment is basically as shown in the attached Figure 1 As shown: A device for detecting carbonyl mycotoxins in food includes an external detection device, which includes a photodetector and a biosensor, and also includes a built-in extraction device, which includes a high-pressure pump and a tank body connected to each other, the top of the tank body is provided with a tank cover 1, and the top of the tank cover 1 is provided with an injection pipe 2 and a pressure pipe 3.

[0033] The interior of the tank body is provided with a coarse filter component and a fine filter component in sequence from top to bottom; an upper space 4 is formed from the coarse filter component to the top of the tank body, a middle space 5 is formed between the coarse filter component and the fine filter component, a bearing space 6 is formed between the fine filter component and the bottom of the refining device, and the tank cover 1, the upper space 4, the middle space 5 and the bearing space 6 are threadedly connected.

[0034] Please refer to Figure 2 and Figure 3 The coarse filter component includes a first filter disc 7 and a first filter membrane 8, and the fine filter component includes a membrane cover 9, a second filter disc 10 and a second filter membrane 11. The top of the membrane cover 9 is provided with a through hole 12. The surfaces of the first filter disc 7 and the second filter disc 10 are provided with a plurality of vertical grids 13. The mesh number of the first filter membrane 8 is greater than the mesh number of the second filter membrane 11. Parallel flow channels 14 are formed between adjacent grids 13. The cross-sectional area of ​​the parallel flow channels 14 is the same as the cross-sectional area of ​​the flow channels connected to the high-pressure pump. The surface of the parallel flow channels 14 has filter holes. The first filter membrane 8 is bonded to the first filter disc 7 of the coarse filter component. The membrane cover 9 and the second filter disc 10 are buckled with each other, and the membrane cover 9 is provided with spacer bars 15 at the position facing the second filter disc 10. The spacer bars 15 are grouped in pairs, and each group of spacer bars 15 is directly opposite to the coverage range of the parallel flow channels 14. The spacer bars 15 and the parallel flow channels 14 form a pressure flow channel 16 with a gap.

[0035] The specific implementation process is as follows: first, put the alcohol and oil foods to be tested into the refining device, then close the refining device, and then continue to pass high pressure. Since the cross-sectional area of ​​the parallel flow channel 14 of the first filter disc 7 is the same as the cross-sectional area of ​​the flow channel connected to the high-pressure pump, the food to be tested flows along the surface of the first filter disc 7 under high pressure. At this time, the pressure gradually causes the food to be tested to flow along the parallel flow channel 14, and then the food to be tested is gradually pressed down through the filter holes by the pressure, and then the macromolecular substances of the food to be tested are retained by the first filter membrane 8, thereby completing the first screening. At this time, the macromolecular substances remain on the surface of the first filter disc 7.

[0036] After the first screening is completed, the food to be tested passes through the membrane cover 9 and enters the surface of the second filter disc 10. At this time, the spacer bars 15 divide the parallel flow channels 14 into a finer portion of the flow channels. At this time, the parallel flow channels 14 and the spacer bars 15 are closed to each other, so that the food to be tested flowing here forms an extruded and closed space, thereby causing the food to be tested to gradually leak out under pressure, thereby increasing the role and effect of extrusion. When the food to be tested passes through the second filter disc 10, the remaining strain components on the surface of the second filter membrane 11, and the remaining water and residues are at the bottom of the tank.

[0037] This technical solution performs stratified treatment on oil and alcohol substances, so as to improve the detection target and sensitivity. At the same time, due to the arrangement of the first filter membrane 8 and the second filter membrane 11, it is easy to form a membrane wrapping structure, which is convenient for subsequent colony culture (especially membrane wrapping culture method) and material detection using a photodetector or biosensor.

[0038] Embodiment 2

[0039] The difference between this embodiment and the above embodiment is that a metering component is provided on one side of the width of the carrying space 6, the metering component includes a metal tube 18 and a prompt circuit, the prompt circuit includes a power supply and a lamp bead 17, and a closed loop is formed in the prompt circuit when the conductive block 20 moves to the disconnected space.

[0040] The top of the metal tube 18 is provided with opposed metal guides 19, and the metal tube 18 has a vertical slide rail 21 built therein, the slide rail 21 is slidably connected to the conductive block 20, the metal guides 19 are connected to the prompt circuit, and a disconnection space is formed between the metal guides 19, and the conductive block 20 is slidably connected inside the metal tube 18, the cross section of the conductive block 20 is trapezoidal, and the inside of the conductive block 20 is hollow, and the outside of the conductive block 20 is made of copper. The width of the conductive block 20 is equal to the width of the disconnection space, and the movement of the conductive block 20 passes through the disconnection space.

[0041] The specific implementation process is as follows: when in use, the gradually accumulated liquid in the carrying space 6 is used to form the working conditions of the metering group. Therefore, when the liquid gradually accumulates, the liquid enters the metal tube 18. At this time, the liquid continuously piles up the conductive block 20 in the metal tube 18. When the conductive block 20 contacts the metal guide plate 19, the prompt circuit is connected. After being connected, the prompt circuit starts to conduct electricity. Therefore, the carried liquid is measured, which is convenient for monitoring the accumulation and filtration of the liquid in a closed high-pressure environment, so as to release the high pressure in time to extend the service life of the device. At the same time, the use of glass material is avoided to prevent the glass material from bursting under pressure.

[0042] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0043] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A device for detecting carbonyl mycotoxins in food, comprising an external detection device, the detection device comprising a spectrometer and a biosensor, characterized in that: Also included is a built-in refining device, the refining device includes a high-pressure pump and a tank body connected to each other, the top of the tank body is provided with a tank cover, and the inside of the tank body is provided with a coarse filter component and a fine filter component in sequence from top to bottom; The coarse filter assembly includes a first filter disc and a first filter membrane, and the fine filter assembly includes a membrane cover, a second filter disc and a second filter membrane. The surfaces of the first filter disc and the second filter disc are provided with a plurality of vertical grids, and parallel flow channels are formed between adjacent grids. The cross-sectional area of ​​the parallel flow channels is the same as the cross-sectional area of ​​the flow channels connected to the high-pressure pump. The surfaces of the parallel flow channels are provided with filter holes. The first filter membrane is bonded to the first filter disc of the coarse filter assembly, and the membrane cover and the second filter disc are buckled with each other. The membrane cover is provided with spacer bars at a position facing the second filter disc. The spacer bars are arranged in groups of two, and each group of spacer bars faces the coverage range of the parallel flow channels. A pressure flow channel with a gap is formed between the spacer bars and the parallel flow channels. A penetration hole is provided on the top of the membrane cover. A bearing space is formed between the fine filter assembly and the bottom of the refining device, a metering assembly is provided on one side of the width of the bearing space, the metering assembly includes a metal tube and a prompt circuit, the top of the metal tube is provided with opposing metal guides, the metal guides are connected to the prompt circuit, and a disconnection space is formed between the metal guides, a conductive block is slidably connected in the metal tube, the width of the conductive block is equal to the width of the disconnection space, and the movement stroke of the conductive block passes through the disconnection space, a vertical slide rail is built into the metal tube, and the slide rail is slidably connected to the conductive block; The prompt circuit includes a power supply and a lamp bead, and when the conductive block moves to the disconnection space, a closed loop is formed in the prompt circuit.

2. The device for detecting carbonyl mycotoxins in food according to claim 1, characterized in that: The cross section of the conductive block is trapezoidal, the inside of the conductive block is hollow, and the outside of the conductive block is made of copper.

3. The device for detecting carbonyl mycotoxins in food according to claim 1, characterized in that: An upper space is formed from the coarse filter assembly to the top of the tank body, a middle space is formed between the coarse filter assembly and the fine filter assembly, and the tank cover, the upper space, the middle space and the bearing space are threadedly connected.

4. The device for detecting carbonyl mycotoxins in food according to claim 1, characterized in that: The top of the tank cover is provided with an injection connection and a pressure connection.

5. The device for detecting carbonyl mycotoxins in food according to claim 1, characterized in that: The mesh number of the first filter membrane is smaller than the mesh number of the second filter membrane.

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