An electrochemical sensing smart cabin integrating environmental parameters

Abstract

The present invention relates to an electrochemical sensing intelligent cabin integrating environmental parameters, including a cabin body of an intelligent cabin for electrochemical sensing detection, wherein a communication control unit, a temperature monitoring unit, a gas detection unit and an electrochemical sensing detection unit are arranged in the cabin body, the communication control unit can communicate with a program control end of an electrochemical sensing detection intelligent control system, the temperature monitoring unit can automatically adjust the temperature in the cabin body, the gas detection unit can detect the carbon dioxide concentration in the cabin body and transmit it to the program control end through the communication control unit, and the electrochemical sensing detection unit can automatically complete the electrochemical detection process under the control of the communication control unit. An environmental parameter monitoring unit capable of monitoring temperature and gas is integrated, and all monitored environmental variables are uploaded to a smart phone for users to view, so as to facilitate subsequent standardized environmental variables. The operating steps in the electrochemical sensor measurement process performed in the cabin body are all automated, which greatly improves the detection efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of electrochemical sensor experimental cabin improvement, in particular to an electrochemical sensor intelligent cabin integrating environmental parameters. Background Art

[0002] Electrochemical detection technology is used in many fields of modern analytical science and has become one of the main and commonly used sensor detection methods. For example, it has a wide range of applications in analytical detection fields such as environmental monitoring, medical diagnosis, and food supervision. Compared with other detection technologies, electrochemical detection technology can convert biological data into easy-to-process electrical signals. It has the characteristics of high sensitivity, low cost, and miniaturization, which makes it have broad application prospects. However, the electrochemical sensor detection system used in general laboratories is expensive, not portable, and has cumbersome operation steps.

[0003] In addition, when we study electrochemical sensors, we often emphasize the modification of sensor electrodes but ignore whether external environmental factors affect the measurement process. In fact, temperature can affect the pH value of the measured solution or directly affect the activity of the electrode surface modification, thereby changing the sensitivity of the sensor electrode. In the strict measurement process, external environmental factors cannot be ignored. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a program control terminal, a communication control unit, a temperature monitoring unit, a gas detection unit and an electrochemical sensor detection unit arranged in a cabin. The program control terminal can communicate with the communication control unit. After the program control terminal sets the temperature in the cabin, the temperature monitoring unit can automatically adjust the temperature in the cabin under the control of the communication control unit. The gas detection unit can transmit the CO2 concentration in the cabin to the communication control unit and the communication control unit transmits it to the program control terminal. The communication control unit can receive the operation instructions of the program control terminal and send them to the electrochemical sensor detection unit, and the electrochemical detection is completed by the electrochemical sensor detection unit, thereby realizing an electrochemical sensor intelligent cabin with integrated environmental parameters that is remotely controlled by the program control terminal and can automatically complete the electrochemical detection process.

[0005] The technical solution adopted by the present invention is:

[0006] A smart cabin for electrochemical sensing detection, comprising a cabin body, wherein a communication control unit, a temperature monitoring unit, a gas detection unit and an electrochemical sensing detection unit are arranged inside the cabin body, the communication control unit can communicate with a program control end, the temperature monitoring unit can automatically adjust the temperature inside the cabin body, the gas detection unit can detect the carbon dioxide concentration inside the cabin body and transmit it to the program control end through the communication control unit, and the electrochemical sensing detection unit can automatically complete the electrochemical detection process under the control of the communication control unit.

[0007] Among them, the electrochemical sensing detection unit includes a pipetting module, a reagent bottle transport module and a sensor carrying module. The pipetting module includes a robotic arm on which a syringe is arranged. The reagent bottle transport module includes a dividing plate for carrying reagent bottles and a rotating drive component for driving the dividing plate to rotate. The sensor carrying module includes a trough-shaped container on which an electrode clamp is arranged.

[0008] The robotic arm comprises a transverse moving part and a lifting part, a syringe is arranged on the lifting part, a piston which can be lifted and lowered is arranged in the syringe, a piston driving motor and a laser rangefinder are arranged on the side of the syringe, the laser rangefinder is connected to the syringe, the piston driving motor can drive the piston and a reflector plate connected to the piston to lift and lower, and the laser rangefinder and the reflector plate can work together to measure the position of the piston in the syringe and the distance it moves.

[0009] Wherein, the reagent bottle conveying module includes a dividing plate, a tray and a rotating drive component. A tray is arranged concentrically with the dividing plate and at an interval below the dividing plate. A rotating drive component is arranged below the tray, and the rotating component can drive the dividing plate and the tray to rotate concentrically.

[0010] Among them, the trough-shaped container of the sensor carrying module is provided with a tip storage tank, an ultrapure water storage tank and a waste liquid storage tank in sequence, a tip detacher is provided above the tip storage tank near one end of the trough-shaped container, and an electrode clamp is provided above the waste liquid storage tank near the other end of the trough-shaped container.

[0011] Among them, the transverse movement component includes a transverse movement track, on which a lifting component capable of moving laterally along the transverse movement track is arranged, a transverse movement drive motor is arranged on one side of the transverse movement track, and the transverse movement drive motor can drive a transverse movement screw installed on the lower end of the lifting component to rotate so that the lifting component can move laterally along the transverse movement track; the lifting component includes a vertical slide, on which a slider capable of moving vertically up and down along the slide is arranged, and a lifting drive motor is arranged on the upper end of the slide, and the lifting drive motor can drive a lifting screw installed in the slider to rotate so that the slider can move vertically up and down along the slide.

[0012] The syringe is fixedly connected to the slider, and the piston of the syringe extends above the slider. The slider is provided with a piston driving motor, which can drive a piston driving screw connected to the upper end of the piston to rotate so that the piston can be raised and lowered in the syringe to absorb or squeeze out the liquid in the syringe; a laser rangefinder is provided on the side of the slider, and a reflector is provided on the side of the upper end of the piston aligned with the laser rangefinder, and the reflector can cooperate with the laser rangefinder to measure the position of the piston in the syringe and the moving distance.

[0013] Among them, a plurality of reagent storage stations are evenly spaced on the dividing plate, a tip storage station is provided between every two adjacent reagent storage stations, and each tip storage station is at the same distance from the two adjacent reagent storage stations on its side. A plurality of slits are evenly spaced on the outer edge of the tray, and the number of the slits is equal to the sum of the number of reagent storage stations and tip storage stations. A slot-shaped photoelectric switch is provided on the side of the tray opposite to the slit, and the slot-shaped photoelectric switch can calculate the angle of rotation of the dividing plate by identifying the number of slits passed.

[0014] Among them, a deflection drive motor is arranged on the side of the electrode clamp, and the deflection drive motor is connected to the electrode clamp through a rotating shaft. The deflection drive motor can drive the electrode clamp to deflect a certain angle through the rotating shaft; a glass tube is arranged above the electrode clamp, and a dry air flow is passed into the glass tube. The air outlet of the glass tube is aligned with the electrode clamped by the electrode clamp so that the dry air flow blown out of the glass tube can dry the electrode.

[0015] Among them, a table is arranged in the cabin, the rear end of the upper end surface of the table is arranged with the transverse track, a tray is arranged above the upper end surface of the table at the front end located on one side of the transverse track, a rotating driving component is arranged on the table below the tray, a trough-shaped container is arranged on the upper end surface of the table at the front end located on the other side of the transverse track, a gas detection unit is arranged on the upper end surface of the table at the front end of the trough-shaped container, a temperature monitoring unit is arranged on the table on the other side of the trough-shaped container, and the temperature monitoring unit includes two heat sinks respectively arranged on the upper end surface and the lower end surface of the table and a semiconductor refrigeration plate located between the two heat sinks.

[0016] An electrochemical sensing detection intelligent control system, which includes a program control terminal, which can communicate with the communication control unit as described above. After the user sets the temperature in the cabin at the program control terminal, the program control terminal sends instructions to the communication control unit and controls the temperature monitoring unit to adjust the temperature in the cabin. The program control terminal can receive the carbon dioxide concentration in the cabin transmitted to the communication control unit by the gas detection unit. When the user selects and starts the electrochemical test at the program control terminal, the program control terminal can send instructions to the communication control unit and control the electrochemical sensing detection unit to automatically complete the electrochemical detection process.

[0017] The advantages and positive effects of the present invention are:

[0018] In the present invention, an electrochemical detection unit is arranged in a sealed cabin, and an environmental parameter monitoring unit including a temperature monitoring unit and a gas monitoring unit is integrated in the cabin. All monitored environmental variables are uploaded to a smart phone for users to view, so as to facilitate the subsequent standardized environmental variables. The temperature monitoring unit uses a semiconductor refrigeration chip combined with an H-bridge driving module with adjustable output voltage and polarity to form a closed-loop negative feedback temperature control system. The semiconductor refrigeration chip can be refrigerated or heated on one side when fixed, and the speed of refrigeration or heating is adjustable, which prevents the circuit from burning out due to excessive circuit current when the initial deviation is too large, and also avoids large temperature fluctuations in the cabin. A series of operation steps required in the electrochemical sensor measurement process in the cabin are all automated, which greatly improves the detection efficiency of the staff. A stepper motor is used as a motion actuator in the electrochemical detection unit, and the precise and controllable movement of the stepper motor greatly reduces the possibility of errors in the electrochemical detection unit. The precise movement of the syringe piston is driven by the lead screw stepper motor, and the position of the piston is determined in conjunction with a laser rangefinder, which avoids the measurement error caused by the target analyte solution entering the syringe. Setting multiple stations on the indexing plate can load 12 reagent bottles and 12 pipette tips at one time, avoiding the tedious steps of "test once, load once", which is especially suitable for the detection of reagents of the same type with different concentrations. Electrochemical sensing detection in a sealed cabin requires fewer use conditions, is easy to operate, has good operating stability, strong versatility, and is easy to promote and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of the present invention;

[0020] Figure 2 yes Figure 1 Left view of

[0021] Figure 3 yes Figure 1 A top view of

[0022] Figure 4 It is a schematic diagram of the electrochemical detection process of the present invention;

[0023] Figure 5 It is the display operation interface of the WeChat applet of the present invention. DETAILED DESCRIPTION

[0024] The present invention is further described below in conjunction with examples. The following examples are illustrative rather than restrictive, and the protection scope of the present invention cannot be limited by the following examples.

[0025] The present invention provides an electrochemical sensing intelligent cabin integrating environmental parameters, comprising an intelligent cabin for electrochemical sensing detection capable of controlling environmental parameters and an electrochemical sensing detection intelligent control system capable of communicating with the intelligent cabin.

[0026] A smart cabin for electrochemical sensing detection, such as Figure 1 , 2 As shown in , 3, the innovation of the present invention lies in: comprising a cabin, the cabin can be sealed, and a communication control unit, a temperature monitoring unit, a gas detection unit and an electrochemical sensor detection unit are arranged in the cabin, the communication control unit can communicate with the program control end, the temperature monitoring unit can automatically adjust the temperature in the cabin, the gas detection unit can detect the carbon dioxide concentration in the cabin and transmit it to the program control end through the communication control unit, and the electrochemical sensor detection unit can automatically complete the electrochemical detection process under the control of the communication control unit.

[0027] A table 28 is arranged in the cabin, and the temperature monitoring unit, gas detection unit and electrochemical sensing detection unit are all arranged on the table. The electrochemical sensing detection unit includes a pipetting module, a reagent bottle conveying module and a sensor carrying module. The pipetting module includes a mechanical arm arranged on the upper end surface of the table. The mechanical arm is a two-degree-of-freedom mechanical arm, and a syringe is arranged on the mechanical arm. The mechanical arm can carry the syringe to load and unload the pipette tip and absorb and drip the reagent; the reagent bottle conveying module includes a dividing plate for carrying the reagent bottle and a rotating driving component for driving the dividing plate to rotate. The sensor carrying module includes a trough-shaped container, and an electrode clamp 45 is arranged above the trough-shaped container 23. The trough-shaped container is used to hold the used pipette tip and the waste liquid after the electrochemical detection, and the electrode clamp is used to clamp the electrode for electrochemical sensing detection.

[0028] The mechanical arm includes a transverse moving part and a lifting part. The transverse moving part includes a transverse moving track 46, and the transverse moving track is arranged on the rear side of the upper end surface of the table. A vertical lifting part is arranged on the transverse moving track, and baffles 33 are arranged at both ends of the transverse moving track respectively. The lifting part can move laterally between the above-mentioned baffles along the transverse moving track. A transverse moving driving motor 36 is arranged at one end of the transverse moving track, and the output shaft of the transverse moving driving motor is connected to a transverse moving lead screw 19 arranged above the transverse moving track. The transverse moving lead screw is installed at the lower end of the lifting part and cooperates with the internal thread arranged at the lower end of the lifting part or the nut arranged at the lower end of the lifting part. The transverse moving driving motor drives the transverse moving lead screw to rotate so as to drive the lifting part to move laterally along the transverse moving track. A guide column spaced and parallel to the lead screw can be arranged at the lower end of the lifting part to enable the lifting part to move smoothly. Other components such as limit grooves, limit blocks, etc. that can enable the lifting part to move smoothly are also possible. The transverse moving driving motor can adopt a stepping motor, so that the distance of the lifting part moving laterally on the transverse moving track can be accurately controlled.

[0029] The lifting component includes a vertical slide 9, and the upper and lower ends of the slide are respectively provided with a top plate 5 and a bottom plate. The bottom plate at the lower end of the slide is provided with the above-mentioned traverse screw or connected with the above-mentioned nut and is provided on the traverse track for lateral limit movement. The slide is provided with a slider that can move along the vertical limit lifting and lowering of the slide, and the top plate of the slide is provided with a lifting drive motor 3. The output shaft of the lifting drive motor is connected with a lifting screw 12 provided on the front side of the slide. The lifting screw is installed in the slider and cooperates with the internal thread (such as a nut) in the slider. It is also possible to provide a nut on the slider. The lifting drive motor drives the lifting screw to rotate, thereby driving the slider to move along the vertical limit lifting and lowering of the slide. The front side of the slide between the slide and the lifting screw is provided with a lifting limit track 40, which can limit the slider so that the slider remains stable when it moves vertically. The lifting drive motor can adopt a stepping motor, so that the vertical movement distance of the slider can be accurately controlled.

[0030] In the present invention, both the traverse drive motor and the lift drive motor use a screw stepper motor with a step angle of 1.8° and a lead of 1mm, and a resolution of 0.005mm. The travel switches are arranged at the extreme positions of the traverse track and the slide table to eliminate the cumulative error, ensuring that the syringe carried by the robot arm can perform a series of operations accurately.

[0031] A connecting plate is provided at the front end of the slider, and a fixing seat 41 is provided on the connecting plate. The fixing seat is a hollow cube type, and a syringe is provided in the fixing seat. The opening of the syringe barrel 10 faces upward and is located at the upper end of the fixing seat. The lower end of the syringe barrel extends below the fixing seat. A suction head seat is provided at the bottom of the syringe barrel, and a suction head 11 for sucking the reagent solution can be installed on the suction head seat. A piston is provided in the upward opening of the syringe, and the lifting and lowering of the piston in the syringe can enable the syringe to suck or squeeze out the reagent solution. The piston is connected to the lower end of a piston rod 43, and the upper end of the piston rod is connected to a lifting plate 42. A plurality of guide posts 2 are provided at the upper end of the fixing seat, and the lifting plate is sleeved on the guide posts and moves along the vertical limit of the guide posts.

[0032] There are three guide posts in this embodiment, and the lifting plate is vertically limited and moved above the fixed seat by the three guide posts. A piston drive motor 8 is arranged in the fixed seat on the front side of the syringe, and the output shaft of the piston drive motor is connected to a piston screw 4, which is installed in the lifting plate and cooperates with the internal thread (such as a nut) or a nut in the lifting plate. The piston drive motor drives the piston screw to rotate to drive the lifting plate to move vertically, and the vertical movement of the lifting plate can drive the piston to rise and fall in the syringe through the piston rod. The guide posts can make the lifting of the piston in the syringe more stable while limiting the lifting plate. The piston drive motor can adopt a stepping motor, so that the distance moved by the piston in the syringe can be accurately controlled to control the amount of reagent liquid drawn by the syringe. In the present invention, the piston drive motor adopts a stepping motor.

[0033] A mounting plate 7 is provided at the other end of the fixing seat, a laser rangefinder 6 is provided on the upper end surface of the mounting plate, a reflector 1 is provided at the other end of the lifting plate in alignment with the laser rangefinder, and the reflector can be lifted and lowered together with the lifting plate, and the laser rangefinder and the reflector can work together to measure the position of the piston in the syringe barrel and the moving distance, thereby measuring the amount of reagent liquid absorbed or squeezed out by the syringe.

[0034] The reagent bottle conveying module includes a dividing plate 37, a tray 35 and a rotating drive component. The dividing plate and the tray are arranged at one end of the traverse track ( Figure 1 A rotating drive component is provided on the upper end surface of the table on the front side (the left side in the figure), and on the table below the dividing plate and the tray.

[0035] A tray is arranged at intervals below the indexing plate, and a gasket 38 is arranged between the indexing plate and the tray to keep a gap between the two. A driven gear 13 is arranged on the lower end surface of the tray, and an upper shaft support 31 is arranged at the lower end of the driven gear. A lower shaft support 29 is arranged at intervals below the upper shaft support, and the lower shaft support is fixedly arranged on the upper end surface of the table through a fixing foot 27. The indexing plate, the tray, the driven gear, the upper shaft support and the lower shaft support are sequentially sleeved on the same rotating shaft 26 from top to bottom, and a bearing 30 is arranged between the upper shaft support and the lower shaft support. The lower shaft support is fixed above the table. The upper shaft support can support the driven gear to rotate freely above the lower shaft support under the load of the bearing, and the driven gear can drive the tray to rotate synchronously. The indexing plate is fixedly arranged above the tray through a plurality of positioning columns 39, and the rotation of the tray can drive the indexing plate to rotate synchronously.

[0036] There are four positioning columns in this embodiment. The rotary drive component includes a rotary drive motor 25 fixedly arranged on the upper end surface of the table through a motor fixing seat 24, and a driving gear 14 is arranged on the output shaft of the rotary drive motor, and the driving gear is meshed with the driven gear. When the rotary drive motor drives the driving gear to rotate, it can drive the driven gear to rotate, and the driven gear can drive the indexing plate and the tray to rotate synchronously. In this embodiment, the rotary drive motor adopts a stepping motor (28 series), but the stepping motor with a step angle of 1.8° cannot complete the conversion of the reagent storage station once every 30° without subdivision. Therefore, here, a pair of driving gears and driven gears with a transmission ratio of 1.8 are used to change its transmission characteristics: the rotary drive motor rotates only 1° for each step angle, and the indexing plate can complete the conversion of the reagent storage station once when the rotary drive motor inputs 30 pulses.

[0037] A plurality of reagent storage stations 51 are evenly spaced on the indexing disk. The reagent storage stations are larger circular holes. A tip storage station 52 is provided between each two adjacent reagent storage stations. The tip storage stations are smaller circular holes. The distance between each tip storage station and the two adjacent reagent storage stations is the same. In this embodiment, 12 reagent storage stations are evenly spaced on the indexing disk. Each reagent storage station can accommodate a reagent bottle. Accordingly, 12 tip storage stations are provided on the indexing disk. The tip storage stations and the reagent storage stations are alternately arranged in sequence. The 24 stations are arranged at equal intervals on the circumference. Each reagent storage station has two tip storage stations next to it. Each tip storage station has two reagent storage stations next to it. The intervals between the reagent storage stations and the tip storage stations are the same. The reagent storage stations are arranged in order on the circumference of the indexing disk according to serial numbers 1 to 12, which is convenient for users to identify the corresponding reagent bottles, so that "one loading, 12 measurements" can be achieved, and the space utilization rate is greatly improved.

[0038] The outer edge of the tray is evenly spaced with multiple slits 50, the number of which is equal to the sum of the number of reagent storage stations and pipette tip storage stations. A slot-shaped photoelectric switch 34 is arranged on the side of the tray in alignment with the slits, and the slot-shaped photoelectric switch is arranged on the upper end surface of the table through a support 32. The slot-shaped photoelectric switch can calculate the angle of rotation of the indexing plate by identifying the number of slits passed. In this embodiment, the number of slits is 24, the slit width is 1 mm, and each slit is aligned with a reagent storage station or a pipette tip storage station. The slits and the slot-shaped photoelectric switch cooperate with each other so that each reagent storage station or pipette tip storage station can be identified by the slit passing the slot-shaped photoelectric switch when it rotates.

[0039] The trough-shaped container 23 of the sensor carrying module is arranged on the upper surface of the table at the front side of the other end of the transverse track. The trough-shaped container is a horizontal rectangular parallelepiped, and a plurality of grooves are provided on the upper surface of the rectangular parallelepiped. In this embodiment, a tip storage slot 49, an ultrapure water storage slot 48 and a waste liquid storage slot 47 are provided in sequence on the upper surface of the trough-shaped container. A tip detacher 15 is provided above the tip storage slot near one end of the trough-shaped container, and an electrode clamp 45 is provided above the waste liquid storage slot near the other end of the trough-shaped container. The tip detacher is provided with a V-shaped bayonet opening toward the other end of the trough-shaped container. The tip can be detached from the syringe after being inserted into the V-shaped bayonet, and the detached tip falls into the tip storage slot. The electrode clamp is used to clamp the sensor electrode for electrochemical detection.

[0040] A rotating shaft seat is provided on the trough-shaped container above the waste liquid storage tank, a rotating shaft 44 is passed through the rotating shaft seat, the rotating shaft is connected to the lower end of the electrode holder, and an electrochemical sensor electrode for electrochemical detection is provided in the electrode holder. A mounting frame 16 is provided on the upper surface of the table plate located at the front side of the waste liquid storage tank, and a deflection drive motor 18 is provided on the mounting frame. The output shaft of the deflection drive motor is connected to the rotating shaft. The deflection drive motor can drive the rotating shaft to rotate a certain angle, thereby driving the electrode holder to deflect a certain angle. After the electrode holder is deflected, the waste liquid on the electrode and in the electrode holder can be dumped into the waste liquid storage tank below it. The deflection drive motor in this embodiment adopts a stepping motor.

[0041] A glass tube 17 is provided on the mounting frame, and a dry air flow passes through the glass tube. The air outlet of the glass tube is located above the electrode holder and aligned with the electrode held by the electrode holder. The dry air flow blown out of the glass tube can dry the electrode.

[0042] In order to enable emergency braking when errors occur in the operation of each moving part, each moving part has a corresponding travel switch or slot-type photoelectric switch at the limit position or target position. By continuously detecting whether these switches are triggered or not triggered within a given time, it is determined whether the operation of the smart cabin is wrong, thereby determining whether the system executes the emergency braking procedure. An alarm device can be set on the cabin body. When emergency braking occurs, the alarm device can prompt the operator to urgently cut off the power or manually reset the electrochemical detection unit through an alarm light and a buzzer.

[0043] A gas detection unit is provided on the upper surface of the table on the front side of the trough-shaped container, and the gas detection unit includes a microcontroller and a carbon dioxide concentration sensor 22. The carbon dioxide concentration sensor in this embodiment adopts an SGP30 metal oxide gas sensor. SGP30 mainly detects the CO2 concentration in the workspace and transmits the data to the microcontroller, which is connected to the SGP30 metal oxide gas sensor via an IIC bus. The microcontroller converts the returned raw data to obtain the true value of the CO2 concentration and transmits it to the communication control module.

[0044] A temperature monitoring unit is arranged on the table on the other side of the trough-shaped container. The temperature monitoring unit includes a 12V lithium battery, a microcontroller, a semiconductor cooling sheet 20, a voltage regulating module and a temperature and humidity sensor. The temperature and humidity sensor in this embodiment adopts an SHT40 digital temperature and humidity sensor. The 12V lithium battery is connected to the voltage regulating module, the PWM signal line of the microcontroller is also connected to the voltage regulating module, the voltage regulating module is connected to the semiconductor cooling sheet, and the SHT40 sensor is connected to the microcontroller. The semiconductor cooling sheet is embedded in the table on the other side of the trough-shaped container, and two heat sinks 21 are arranged on the upper and lower end surfaces of the semiconductor cooling sheet. The voltage regulating module can output a variable voltage from -12V to 12V under the control of the microcontroller PWM signal. The user first needs to set a target temperature, measure the actual temperature and humidity through the SHT40 digital temperature and humidity sensor and feed the actual temperature and humidity back to the microcontroller. The microcontroller calculates and processes the deviation between the actual temperature and the target temperature and outputs it to the actuator (semiconductor refrigeration chip and voltage regulation module) to control the temperature in the entire smart cabin. This deviation will cause the actuator to continue to work until the actual temperature of the system reaches the target temperature value with a small fluctuation.

[0045] A communication control unit is also provided in the cabin, and the communication control unit includes a microcontroller based on STM32F415RGT6 and a Bluetooth module. The Bluetooth module receives the control instruction and transmits it to the microcontroller. The microcontroller, as a control and data processing unit, sends control signals to each actuator and receives data sent back by each sensor, so as to finally coordinate the operation of each moving part in the smart cabin. The communication control unit can be provided on the inner wall of the cabin or on the platform. In this embodiment, the communication control unit is provided on the platform.

[0046] An electrochemical sensing detection intelligent control system comprises a program control terminal, which can communicate with a communication control unit in the above-mentioned intelligent cabin for electrochemical sensing detection.

[0047] Since the electrochemical sensing detection smart cabin is not fully automatic when working, the loading of reagents and tips needs to be done manually, so the preferred communication method in this embodiment is Bluetooth connection, and other wireless communication methods such as WIFI or wired communication methods can also be selected according to the actual environment. The preferred program control terminal in this embodiment is mounted on a smart phone and runs in the form of a WeChat applet, which is convenient and fast, and the communication control unit can display the data detected by the electrochemical sensor in real time in the WeChat applet.

[0048] like Figure 5As shown in the figure, the WeChat applet includes a communication establishment interface, a parameter setting and control interface, and a drawing and data display interface. The communication establishment interface includes switch buttons for "turn on Bluetooth" and "turn off Bluetooth", and has the function of pulling down to refresh Bluetooth devices. In the Bluetooth device display list, a Bluetooth signal strength symbol is designed, which is divided into four strength levels. A full grid indicates the strongest signal. After Bluetooth is connected, a "disconnect Bluetooth" button is displayed to facilitate flexible use by users.

[0049] The parameter setting and control interface includes the numerical display of the temperature, humidity, and carbon dioxide concentration in the smart cabin, as well as an input box for manually setting the target temperature of the smart cabin. A selector for the electrochemical test method is provided at the bottom of the interface. After selecting the electrochemical test method, the interface will be redirected to the electrochemical test parameter setting interface. After the parameter setting is completed, click the "OK" button to exit the interface. Click the "START" button to start the electrochemical test process. After clicking, the interface will automatically enter the data display and drawing interface.

[0050] The drawing and data display interface includes a drawing area at the top, which shows the curve of current with respect to potential; two areas, a receiving area and a sending area, and related buttons are set at the bottom. The sending area allows the user to manually enter the command code and send it to the lower computer to test the device. The receiving area is the data display area, where the user can see the specific values ​​of the electrochemical test in real time. There is also a "clear data" button below the data display area to facilitate users to perform related operations.

[0051] The program control end can set the temperature in the smart cabin. After setting, the program control end sends instructions to the communication control unit, and the communication control unit controls the temperature monitoring unit to adjust the temperature in the cabin to the set value. The gas detection unit can transmit the detected concentration of carbon dioxide in the cabin to the communication control unit, and then the communication control unit transmits it to the program control end. The user can view the gas environment in the cabin on the program control end. The accuracy of the electrochemical detection results can be judged by the temperature and gas environment. The error of the electrochemical detection can be controlled or adjusted according to the environmental value to make the detection result more accurate.

[0052] After the user selects the electrochemical test on the program control end, he presses the "START" button. At this time, the program control end sends a command to the communication control unit, and the program control end controls the electrochemical sensing detection unit to automatically complete the electrochemical detection process.

[0053] like Figure 4 As shown, the electrochemical detection process in the present invention is:

[0054] 1. Open the cabin, place the reagent bottles and pipette tips containing pre-configured standard reagents on the indexing plate in order (reagent bottle-pipette tip-reagent bottle-pipette tip), and check whether the components in the cabin are damaged or abnormal;

[0055] 2. Close the cabin, establish a Bluetooth connection between your smartphone and the smart cabin, and view the environmental parameters inside the cabin through the WeChat applet;

[0056] 3. Set the temperature in the cabin to 25°C in the WeChat applet, select DPV as the electrochemical detection method, and enter the parameters required for the electrochemical detection method: starting reference voltage -0.4V, ending reference voltage 0.4V, increment 0.005V, amplitude 0.05V, pulse width 0.05s, period 0.5s;

[0057] 4. Press the "START" button to check whether the components of the electrochemical detection unit are reset. After reset, the electrochemical detection process begins;

[0058] 5. The lifting drive motor drives the syringe down, the suction head seat at the bottom of the syringe is inserted into the suction head on the indexing plate, and the lifting drive motor drives the syringe to carry the suction head up;

[0059] 6. The rotary drive motor drives the indexing plate to rotate one station (i.e., from the current slit to the next slit), the lifting motor drives the syringe down, and the pipette tip carried by the syringe is inserted into the reagent solution in the reagent bottle;

[0060] 7. The piston drive motor drives the piston to rise, the syringe absorbs the reagent liquid and determines the amount of the absorbed reagent liquid through the laser rangefinder;

[0061] 8. The lifting motor drives the syringe to rise, and the traverse drive motor drives the slide and the syringe to move to the top of the electrode holder;

[0062] 9. The piston drive motor drives the piston to descend, and the reagent solution in the syringe is completely dripped onto the electrochemical sensor electrode clamped by the electrode holder for electrochemical detection;

[0063] 10. The traverse drive motor drives the slide and the syringe to move to the top of the tip detacher, and the lifting drive motor drives the syringe to descend. After the tip of the syringe descends and is inserted into the V-shaped bayonet of the tip detacher, the traverse drive motor drives the slide and the syringe to move to the tip of the V-shaped bayonet so that the tip is clamped by the tip detacher, and the lifting drive motor drives the syringe to rise so that the tip is detached from the syringe;

[0064] 11. The traverse drive motor drives the slide and the syringe to move to the top of the ultrapure water storage tank, the lifting drive motor drives the syringe down and inserts the bottom of the syringe into the ultrapure water storage tank, the piston drive motor drives the piston up, and the syringe absorbs the ultrapure water in the ultrapure water storage tank;

[0065] 12. The lifting drive motor drives the syringe to rise, the traverse drive motor drives the slide and the syringe to move above the electrode holder, the piston drive motor drives the piston to descend, and the ultrapure water in the syringe is dripped onto the electrode to rinse the electrode;

[0066] 13. The deflection drive motor drives the electrode holder to deflect. After the waste liquid in the electrode holder is discharged, the deflection drive motor drives the electrode holder to reset, and the glass tube blows out a dry airflow to dry the electrode;

[0067] 14. The rotating drive motor drives the indexing plate to rotate one station, and the above steps 5 to 13 are repeated until all reagent bottles on the indexing plate are used and enter the standby state. Reagents can be reloaded to continue electrochemical testing, or the power can be turned off, and the smart cabin can be opened and cleaned.

[0068] The above process is an electrochemical detection based on the DPV method, which is only an example. The electrochemical sensing detection and analysis that integrates detection process automation and data image analysis on the smartphone platform has far-reaching significance. This technology can be applied to the detection process of electrochemical sensors using screen-printed electrodes. It has the advantages of good device stability and convenient operation, and can meet the requirements of most electrochemical sensor detection.

[0069] In the present invention, an electrochemical detection unit is arranged in a sealed cabin, and an environmental parameter monitoring unit including a temperature monitoring unit and a gas monitoring unit is integrated in the cabin. All monitored environmental variables are uploaded to a smart phone for users to view, so as to facilitate the subsequent standardized environmental variables. The temperature monitoring unit uses a semiconductor refrigeration chip combined with an H-bridge driving module with adjustable output voltage and polarity to form a closed-loop negative feedback temperature control system. The semiconductor refrigeration chip can be refrigerated or heated on one side when fixed, and the speed of refrigeration or heating is adjustable, which prevents the circuit from burning out due to excessive circuit current when the initial deviation is too large, and also avoids large temperature fluctuations in the cabin. A series of operation steps required in the electrochemical sensor measurement process in the cabin are all automated, which greatly improves the detection efficiency of the staff. A stepper motor is used as a motion actuator in the electrochemical detection unit, and the precise and controllable movement of the stepper motor greatly reduces the possibility of errors in the electrochemical detection unit. The precise movement of the syringe piston is driven by the lead screw stepper motor, and the position of the piston is determined in conjunction with a laser rangefinder, which avoids the measurement error caused by the target analyte solution entering the syringe. Setting multiple stations on the indexing plate can load 12 reagent bottles and 12 pipette tips at one time, avoiding the tedious steps of "test once, load once", which is especially suitable for the detection of reagents of the same type with different concentrations. Electrochemical sensing detection in a sealed cabin requires fewer use conditions, is easy to operate, has good operating stability, strong versatility, and is easy to promote and use.

Claims

1. An intelligent cabin for electrochemical sensing detection, characterized in that: It comprises a cabin, in which a communication control unit, a temperature monitoring unit, a gas detection unit and an electrochemical sensing detection unit are arranged, wherein the communication control unit can communicate with a program control terminal, the temperature monitoring unit can automatically adjust the temperature in the cabin, the gas detection unit can detect the carbon dioxide concentration in the cabin and transmit it to the program control terminal through the communication control unit, and the electrochemical sensing detection unit can automatically complete the electrochemical detection process under the control of the communication control unit; The electrochemical sensing detection unit includes a pipetting module, a reagent bottle conveying module and a sensor carrying module, wherein the pipetting module includes a mechanical arm on which a syringe is arranged, the reagent bottle conveying module includes a dividing plate for carrying reagent bottles and a rotating driving component for driving the dividing plate to rotate, and the sensor carrying module includes a slot-shaped container, above which an electrode holder is arranged; The mechanical arm includes a traverse component and a lifting component, a syringe is arranged on the lifting component, a piston capable of lifting and lowering is arranged in the syringe, a piston driving motor and a laser rangefinder are arranged beside the syringe, the laser rangefinder is connected to the syringe, the piston driving motor can drive the piston and a reflector connected to the piston to lift and lower, and the laser rangefinder and the reflector can work together to measure the position of the piston in the syringe and the moving distance; The reagent bottle conveying module comprises a dividing plate, a tray and a rotating driving component. A tray is arranged concentrically with and spaced from the dividing plate below the dividing plate. A rotating driving component is arranged below the tray. The rotating component can drive the dividing plate and the tray to rotate concentrically. The trough-shaped container of the sensor carrying module is provided with a tip storage tank, an ultrapure water storage tank and a waste liquid storage tank in sequence, a tip detacher is provided above the tip storage tank near one end of the trough-shaped container, and an electrode holder is provided above the waste liquid storage tank near the other end of the trough-shaped container; The transverse moving part comprises a transverse moving track, on which a lifting part capable of transversely moving along the transverse moving track is arranged, a transverse moving driving motor is arranged on one side of the transverse moving track, and the transverse moving driving motor can drive a transverse moving screw installed at the lower end of the lifting part to rotate so that the lifting part can be transversely moved along the transverse moving track; the lifting part comprises a vertical slide, on which a slider capable of vertically lifting and moving along the slide is arranged, and a lifting driving motor is arranged at the upper end of the slide, and the lifting driving motor can drive a lifting screw installed in the slider to rotate so that the slider can be vertically lifted and moved along the slide; the syringe is fixedly connected to the slider, and the piston of the syringe extends above the slider, and a piston driving motor is arranged on the slider, and the piston driving motor can drive a piston driving screw connected to the upper end of the piston to rotate so that the piston can be lifted and lowered in the syringe to absorb or squeeze out the liquid in the syringe; a laser rangefinder is arranged on the side of the slider, and a reflector is arranged on the side of the upper end of the piston aligned with the laser rangefinder, and the reflector can cooperate with the laser rangefinder to measure the position of the piston in the syringe and the moving distance; The indexing disk is evenly spaced with a plurality of reagent storage stations, a tip storage station is provided between every two adjacent reagent storage stations, and each tip storage station is at the same distance from the two reagent storage stations adjacent to it; a plurality of slits are evenly spaced with a plurality of slits on the outer edge of the tray, and the number of the slits is equal to the sum of the number of the reagent storage stations and the tip storage stations; a slot-shaped photoelectric switch is provided on the side of the tray in alignment with the slits, and the slot-shaped photoelectric switch can calculate the angle of rotation of the indexing disk by identifying the number of slits passed; A deflection drive motor is arranged beside the electrode holder, and the deflection drive motor is connected to the electrode holder through a rotating shaft, and the deflection drive motor can drive the electrode holder to deflect a certain angle through the rotating shaft; a glass tube is arranged above the electrode holder, and a dry airflow is introduced into the glass tube, and the air outlet of the glass tube is aligned with the electrode clamped by the electrode holder so that the dry airflow blown out of the glass tube can dry the electrode; A table is arranged in the cabin, the transverse track is arranged at the rear end of the upper end surface of the table, a tray is arranged above the upper end surface of the table at the front end on one side of the transverse track, a rotating driving component is arranged on the table below the tray, a trough-shaped container is arranged on the upper end surface of the table at the front end on the other side of the transverse track, a gas detection unit is arranged on the upper end surface of the table at the front end of the trough-shaped container, a temperature monitoring unit is arranged on the table on the other side of the trough-shaped container, and the temperature monitoring unit includes two heat sinks respectively arranged on the upper end surface and the lower end surface of the table and a semiconductor refrigeration plate located between the two heat sinks.

2. An electrochemical sensing detection intelligent control system, characterized in that: The program control terminal includes a program control terminal, which can communicate with the communication control unit as described in claim 1. After the user sets the temperature in the cabin at the program control terminal, the program control terminal sends instructions to the communication control unit and controls the temperature monitoring unit to adjust the temperature in the cabin. The program control terminal can receive the carbon dioxide concentration in the cabin transmitted to the communication control unit by the gas detection unit. When the user selects and starts the electrochemical test at the program control terminal, the program control terminal can send instructions to the communication control unit and control the electrochemical sensing detection unit to automatically complete the electrochemical detection process.

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