Method and device for screening excellent bromegrass germplasm based on efficient nitrogen utilization

Abstract

The present invention relates to artificial intelligence technology, and discloses a method for screening excellent germplasms of Bromus inermis based on efficient nitrogen utilization, including: dividing preset N Bromus inermis seedlings into M experimental groups and planting them in an experimental field, selecting M kinds of nitrogen fertilizers with different concentrations, and applying them to the experimental fields of the M experimental groups respectively; collecting P Bromus inermis samples from each experimental field, detecting the nitrogen fertilizer content in the sample soil and the forage yield of the sample forage, and calculating the weighted average to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field; calculating the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the Bromus inermis samples in each experimental field; and screening out excellent germplasms according to the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity. In addition, the present invention also relates to blockchain technology, and the nitrogen fertilizer content in the soil and the forage yield of the samples can be stored in the nodes of the blockchain. The present invention also proposes a screening device for Bromus inermis based on efficient nitrogen utilization. The present invention can improve the forage yield of Bromus inermis, reduce the amount of nitrogen fertilizer used and production costs.

Description

Technical Field

[0001] The present invention relates to the field of artificial intelligence technology, and in particular to a method and device for screening excellent germplasm of awnless bromegrass based on efficient nitrogen utilization. Background Art

[0002] As the current animal husbandry industry becomes increasingly developed, the demand for high-quality forage is also increasing. The shortage of high-quality forage is one of the key bottleneck issues restricting the high-quality development of animal husbandry. Bromus inermis is a high-quality forage that livestock like to eat because of its high yield, rich nutrition and good palatability. Screening and breeding bromus inermis varieties with strong adaptability, higher forage yield and better nutritional quality is of great significance for the development of high-quality forage production and supply and the promotion of sustainable development of animal husbandry.

[0003] At present, the production of high-quality forage in my country is mainly based on low-quality land such as general cultivated land, saline-alkali land, abandoned land, and retired farmland. The nutritional status of these lands is poor, and nitrogen and phosphorus deficiencies often occur. How to achieve high-quality and high-yield forage on lands that are deficient in nitrogen and phosphorus is an important issue that needs to be solved urgently. Screening for high-quality germplasm of awnless brome with high nitrogen efficiency and cultivating new varieties of nitrogen-efficient awnless brome is an effective way to solve this problem. Summary of the invention

[0004] The invention provides a method and a device for screening excellent germplasm of awnless brome based on efficient nitrogen utilization, and the main purpose is to solve the problems of low nitrogen utilization efficiency and low forage yield of awnless brome.

[0005] To achieve the above object, the present invention provides a method for screening superior germplasm of Bromus inermis based on efficient nitrogen utilization, comprising:

[0006] Select N different varieties or wild materials of smooth brome seedlings, divide the N kinds of smooth brome seedlings into M groups of control experiments, each group has N different varieties of smooth brome seedlings;

[0007] Plant M control experiments in M*N*4 experimental fields with the same nutritional basis, select M nitrogen fertilizers with different concentrations and apply them in the experimental fields of M control experiments;

[0008] After a preset time period, in M*N*4 experimental fields, P awnless brome samples of each experimental field are collected by using a spiral sampling method, and the soil nitrogen fertilizer content and sample forage yield of the P awnless brome samples are detected and weighted averaged to obtain the final nitrogen fertilizer content and final forage yield of the experimental field;

[0009] Calculate the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the smooth brome sample in each experimental field according to the final nitrogen fertilizer content and the final forage yield;

[0010] Identify the bromegrass varieties or germplasm materials with nitrogen fertilizer utilization rate and nitrogen fertilizer productivity greater than the preset utilization rate threshold and productivity threshold in the M control groups as high-quality germplasm.

[0011] Optionally, the selection of M different concentrations of nitrogen fertilizers and their application to the experimental fields in the M control experiments includes:

[0012] Within the preset nitrogen fertilizer concentration threshold range, select a nitrogen concentration value every preset interval, and a total of M values are selected;

[0013] Apply nitrogen fertilizers to the experimental fields in the M control experiments according to the M nitrogen concentration values.

[0014] Optionally, the use of the spiral sampling method to collect P bromegrass samples from each experimental field includes:

[0015] Draw a spiral line in the preset experimental field model diagram according to the spiral line formula;

[0016] Take the center of the experimental field as the fixed point of the spiral line and calculate the arc length of the spiral line;

[0017] Divide the arc length into P parts, and take bromegrass samples of the same area in the experimental fields corresponding to each arc length segment.

[0018] Optionally, the drawing of the spiral line in the preset experimental field model diagram according to the spiral line formula includes:

[0019] Draw a spiral line in the preset experimental field model diagram using the following spiral line formula:

[0020] θ = ωt

[0021]

[0022] where ρ is the spiral line, v is the rotational speed of the fixed point of the spiral line in polar coordinates, ω is the rotational angular velocity of the fixed point of the spiral line in polar coordinates, t is the time at t, and θ is the angle rotated at time t.

[0023] Optionally, the calculation of the arc length of the spiral line with the center of the experimental field as the fixed point of the spiral line includes:

[0024] Calculate the arc length of the spiral line using the following arc length calculation formula:

[0025]

[0026] where s is the arc length of the spiral line, v is the rotational speed of the fixed point of the spiral line in polar coordinates, ω is the rotational angular velocity of the fixed point of the spiral line in polar coordinates, n is the number of half turns the spiral line has turned, and θ is the angle by which the fixed point of the spiral line rotates.

[0027] Optionally, calculating the weighted average of the soil nitrogen fertilizer content and the sample forage yield of the P smooth bromegrass samples to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field, including:

[0028] Detecting the sample soil nitrogen fertilizer content of the P smooth bromegrass samples one by one according to the soil detection method;

[0029] Weighing the P smooth bromegrass samples to calculate the sample forage yield;

[0030] Obtaining the preset nitrogen fertilizer weights of the P sample soil nitrogen fertilizer contents, calculating the nitrogen fertilizer weighted average of the nitrogen fertilizer weights, and determining the nitrogen fertilizer weighted average as the final nitrogen fertilizer content of the experimental field;

[0031] Optionally, the obtaining the preset nitrogen fertilizer weights of the P sample soil nitrogen fertilizer contents and calculating the nitrogen fertilizer weighted average of the nitrogen fertilizer weights includes:

[0032] Calculating the nitrogen fertilizer weighted average of the nitrogen fertilizer weights by using the following weighted average formula:

[0033]

[0034] where X is the nitrogen fertilizer weighted average, P is the number of samples, x i is the soil nitrogen fertilizer content of the i-th sample, f i is the preset nitrogen fertilizer weight of the i-th sample, and i is the number of the currently calculated sample.

[0035] Optionally, calculating the nitrogen fertilizer utilization rate and the nitrogen fertilizer productivity of the smooth bromegrass samples in each test field according to the final nitrogen fertilizer content and the final forage yield includes:

[0036] Calculating the nitrogen fertilizer utilization rate of the smooth bromegrass samples in the test field by using the following nitrogen fertilizer utilization rate formula:

[0037]

[0038] where AE is the nitrogen fertilizer utilization rate of the smooth bromegrass samples, α is the final forage yield of the test field corresponding to the smooth bromegrass samples, β is the final forage yield of the test field without applying nitrogen fertilizer preset, and γ is the final nitrogen fertilizer content of the test field corresponding to the smooth bromegrass samples.

[0039] Optionally, calculating the nitrogen fertilizer utilization rate and the nitrogen fertilizer productivity of the smooth bromegrass samples in each test field according to the final nitrogen fertilizer content and the final forage yield includes:

[0040] Calculating the nitrogen fertilizer productivity of the smooth bromegrass samples in the test field by using the following nitrogen fertilizer productivity formula:

[0041]

[0042] Among them, PFP is the nitrogen fertilizer productivity of the smooth bromegrass sample, γ is the final nitrogen fertilizer content of the experimental field corresponding to the smooth bromegrass sample, and β is the final forage yield of the experimental field without applying nitrogen fertilizer preset.

[0043] To solve the above problems, the present invention also provides a smooth bromegrass screening device based on efficient nitrogen utilization, and the device includes:

[0044] Seed selection module: Select smooth bromegrass seedlings of N different varieties or wild materials, and divide the N smooth bromegrass seedlings into M groups of control experiments, and each group has smooth bromegrass seedlings of N different varieties;

[0045] Experiment module: Plant the M groups of control experiments into M*N*4 experimental fields with the same nutritional basis respectively, select M different concentrations of nitrogen fertilizers, and apply them to the experimental fields of the M groups of control experiments respectively;

[0046] First calculation module: After a preset time period, in the M*N*4 experimental fields, use the spiral sampling method to collect P smooth bromegrass samples from each experimental field, detect the soil nitrogen fertilizer content and the sample forage yield of the P smooth bromegrass samples, and perform weighted averaging to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field;

[0047] Second calculation module: Calculate the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the smooth bromegrass samples in each experimental field according to the final nitrogen fertilizer content and the final forage yield;

[0048] Final determination module: Determine the smooth bromegrass varieties or germplasm materials with nitrogen fertilizer utilization rate and nitrogen fertilizer productivity both greater than the preset utilization rate threshold and productivity threshold in the M groups of control groups as high-quality germplasms.

[0049] In the embodiment of the present invention, P smooth bromegrass samples are collected from each experimental field by the spiral sampling method, the soil nitrogen fertilizer content and the sample forage yield of the P smooth bromegrass samples are detected and weighted averaged to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field, which is beneficial to improving the calculation accuracy, and then calculating the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity according to the calculated final nitrogen fertilizer content and the final forage yield; screening out high-quality and excellent smooth bromegrass varieties according to the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity is convenient for increasing the forage yield of smooth bromegrass. Therefore, the smooth bromegrass excellent germplasm screening method and device based on efficient nitrogen utilization proposed by the present invention can solve the problem of low forage yield of smooth bromegrass. Description of the Drawings

[0050] Figure 1Schematic flow chart of a method for screening excellent germplasms of Bromus inermis based on efficient nitrogen utilization provided by an embodiment of the present invention;

[0051] Figure 2 Schematic flow chart of a method for obtaining Bromus inermis samples provided by an embodiment of the present invention;

[0052] Figure 3 Schematic flow chart of a method for selecting and calculating the final nitrogen fertilizer content and the final forage yield provided by an embodiment of the present invention;

[0053] Figure 4 Functional module diagram of a screening device for Bromus inermis based on efficient nitrogen utilization provided by an embodiment of the present invention;

[0054] The implementation, functional features, and advantages of the objectives of the present invention will be further described in conjunction with the embodiments with reference to the accompanying drawings. Detailed implementation manners

[0055] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0056] An embodiment of the present application provides a method for screening excellent germplasms of Bromus inermis based on efficient nitrogen utilization. The execution subject of the method for screening excellent germplasms of Bromus inermis based on efficient nitrogen utilization includes, but is not limited to, at least one of electronic devices such as a server, a terminal, etc. that can be configured to execute the method provided by the embodiment of the present application. In other words, the method for screening excellent germplasms of Bromus inermis based on efficient nitrogen utilization can be executed by software or hardware installed on a terminal device or a server device, and the software can be a blockchain platform. The server includes, but is not limited to: a single server, a server cluster, a cloud server, or a cloud server cluster, etc. The server can be an independent server or a cloud server that provides basic cloud computing services such as cloud services, cloud databases, cloud computing, cloud functions, cloud storage, network services, cloud communications, middleware services, domain name services, security services, Content Delivery Network (CDN), and big data and artificial intelligence platforms.

[0057] Refer to Figure 1 As shown, it is a schematic flow chart of a method for screening excellent germplasms of Bromus inermis based on efficient nitrogen utilization provided by an embodiment of the present invention. In this embodiment, the method for screening excellent germplasms of Bromus inermis based on efficient nitrogen utilization includes:

[0058] S1. Select N seedlings of Bromus inermis of different varieties or wild materials, and divide the N seedlings of Bromus inermis into M groups of control experiments, with each group having N seedlings of different varieties of Bromus inermis;

[0059] In the embodiments of the present invention, the Bromus inermis seedlings are herbaceous plant seedlings of the Poaceae family and are the most widely cultivated Bromus forage grasses at present. Due to the strong adaptability and good regenerative ability of Bromus inermis, it is an excellent experimental subject. Moreover, Bromus inermis has high nutritional value, providing feed for the vast livestock industry and having high research and practical value.

[0060] In the embodiments of the present invention, N Bromus inermis seedlings of different varieties or wild materials are selected. Since many cultivated varieties have been bred since the breeding improvement of Bromus inermis began, it is necessary to screen different varieties of Bromus inermis, such as Xinque No. 1, Wusu No. 1, and Xilinguole Bromus inermis, etc.

[0061] In the embodiments of the present invention, the N Bromus inermis seedlings are divided into M groups of control experiments, and each group has N Bromus inermis seedlings of different varieties. For example, there are currently five Bromus inermis seedlings of different varieties, which are divided into two control groups for experiments, and each control group has the same five Bromus inermis seedlings of different varieties.

[0062] Specifically, different varieties of Bromus inermis are studied by setting up control groups, and the growth environments of different varieties of Bromus inermis are controlled. For example, in the M groups of control experiments, it can be M experimental fields with different soil environments. The Bromus inermis seedlings of different varieties are planted in the soils with different growth conditions, which is convenient for analyzing the most suitable soil environment for growth and the Bromus inermis variety with the best growth.

[0063] S2. Plant the M groups of control experiments into M * N * 4 experimental fields with the same nutritional basis respectively, select M different concentrations of nitrogen fertilizers, and apply them to the experimental fields of the M groups of control experiments respectively;

[0064] In the embodiments of the present invention, the experimental field is a farmland for agricultural technicians to conduct projects such as variety comparison, fertilizer comparison, season comparison, pest and disease control comparison, etc. In this case, it is a farmland for variety comparison.

[0065] Specifically, the nitrogen fertilizer is a fertilizer mainly composed of nitrogen elements. There are many types of nitrogen fertilizers, but their functions are basically the same. They can provide the necessary nutrients for the growth of plants and are beneficial to most plants. However, too concentrated nitrogen fertilizer will also inhibit the growth of plants.

[0066] Specifically, the control experiment is an experiment in which, when exploring the influence of a certain condition on the research object, the research object is made to have the same other conditions except that this condition is different. According to the variable, a group of control experiments is set up to make the experimental results more persuasive.

[0067] In the embodiment of the present invention, the M groups of control experiments are planted in M*N*4 experimental fields with the same nutritional basis. For example, N is 3 and M is 2, that is, there are 3 different varieties of awnless brome, which are to be divided into two groups for experiments. A total of 2*3, i.e., 6 experimental fields with the same soil basis are required to ensure that each type of awnless brome can be planted in two different experimental fields to form a control experiment.

[0068] In an embodiment of the present invention, the selecting of M nitrogen fertilizers of different concentrations and applying them respectively in experimental fields of M groups of control experiments includes: within a preset nitrogen fertilizer concentration threshold range, selecting a nitrogen concentration value every preset interval, taking a total of M; and applying nitrogen fertilizer in the M groups of control experimental fields according to the M nitrogen concentration values.

[0069] In detail, since the addition of nitrogen fertilizer will also increase the content of other elements, for example, if the nitrogen fertilizer is calcium nitrate, the concentration of calcium fertilizer will also increase while the nitrogen fertilizer concentration is supplemented, which destroys the principle of the control experiment, increases the variables of the control experiment, and causes errors in the result analysis. Therefore, in the experimental field with low nitrogen fertilizer concentration, additional calcium fertilizer is needed to control the variables and complete the establishment of the control experiment.

[0070] S3. After a preset time period, in M*N*4 experimental fields, P awnless brome samples of each experimental field are collected by spiral sampling method, and the soil nitrogen fertilizer content and sample forage yield of the P awnless brome samples are detected and weighted averaged to obtain the final nitrogen fertilizer content and final forage yield of the experimental field;

[0071] In the embodiment of the present invention, the preset time period is the period when most of the awnless brome in the experimental fields are mature, which is convenient for harvesting.

[0072] In the embodiment of the present invention, the spiral line is a curve formed by spiraling outwards in circles starting from a fixed point.

[0073] For details, refer to Figure 2 As shown, the spiral sampling method is used to collect P awnless brome samples from each experimental field, including:

[0074] S21. Draw a spiral line in a preset experimental field model diagram according to the spiral line formula;

[0075] S22, taking the center of the experimental field as the fixed point of the spiral line, calculating the arc length of the spiral line;

[0076] S23, dividing the arc length into P parts, and taking awnless brome samples of the same area from the experimental field corresponding to each arc length.

[0077] Specifically, drawing a spiral line in a preset experimental field model diagram according to the spiral line formula includes:

[0078] Use the following spiral formula to draw a spiral in the preset experimental field model diagram:

[0079] θ = ωt

[0080]

[0081] Where ρ is the spiral, v is the rotational speed of the fixed point of the spiral in polar coordinates, ω is the rotational angular velocity of the fixed point of the spiral in polar coordinates, t is the time at t, and θ is the angle rotated at time t.

[0082] Specifically, taking the center of the experimental field as the fixed point of the spiral and calculating the arc length of the spiral includes:

[0083] Use the following arc length calculation formula to calculate the arc length of the spiral:

[0084]

[0085] Where s is the arc length of the spiral, v is the rotational speed of the fixed point of the spiral in polar coordinates, ω is the rotational angular velocity of the fixed point of the spiral in polar coordinates, n is the number of half turns the spiral has turned, and θ is the angle the fixed point of the spiral has rotated.

[0086] Specifically, determining the sampling distance according to the calculated arc length is more average and scientific, can truly reflect the growth state of Bromus inermis in the experimental field, and the sample distribution is uniform, with strong representativeness and small sampling error.

[0087] In the embodiment of the present invention, referring to Figure 3 As shown, detecting the soil nitrogen fertilizer content of the P Bromus inermis samples and the sample forage yield and calculating the weighted average to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field includes:

[0088] S31. Detect the sample soil nitrogen fertilizer content of the P Bromus inermis samples one by one according to the soil detection method;

[0089] S32. Weigh the P Bromus inermis samples and calculate the sample forage yield according to the forage yield;

[0090] S33. Obtain the preset nitrogen fertilizer weight of the P sample soil nitrogen fertilizer contents, calculate the nitrogen fertilizer weighted average of the nitrogen fertilizer weights, and determine the nitrogen fertilizer weighted average as the final nitrogen fertilizer content of the experimental field;

[0091] In the embodiment of the present invention, when detecting the soil nitrogen fertilizer content of the P Bromus inermis samples one by one according to the soil detection method, the soil analysis method includes but is not limited to potentiometry, chromic acid redox titration method, correction factor method, indophenol blue colorimetric method, etc.

[0092] Specifically, the preset nitrogen fertilizer weight for obtaining the nitrogen fertilizer content of P sample soils, and calculating the nitrogen fertilizer weighted average value of the nitrogen fertilizer weight includes:

[0093] Calculating the nitrogen fertilizer weighted average value of the nitrogen fertilizer weight by using the following weighted average formula:

[0094]

[0095] Wherein, X is the nitrogen fertilizer weighted average value, P is the number of samples, x i is the nitrogen fertilizer content of the soil of the i-th sample, f i is the preset nitrogen fertilizer weight of the i-th sample, and i is the number of samples currently calculated.

[0096] Specifically, by calculating the forage yield of the samples, the final forage yield of the entire experimental field is deduced according to the weight, and by calculating the nitrogen fertilizer content of the sample soil, the final nitrogen fertilizer content of the experimental field is deduced according to the weight. The calculation method is simple, the calculation steps are concise and not easy to make mistakes, greatly reducing the waste of human resources, and at the same time avoiding the errors that may occur in large-scale calculations of human resources.

[0097] S4. Calculate the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the smooth bromegrass samples in each test field according to the final nitrogen fertilizer content and the final forage yield;

[0098] In the embodiment of the present invention, calculating the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the smooth bromegrass samples in each test field according to the final nitrogen fertilizer content and the final forage yield includes:

[0099] Calculating the nitrogen fertilizer utilization rate of the smooth bromegrass samples in the test field by using the following nitrogen fertilizer utilization rate formula:

[0100]

[0101] Wherein AE is the nitrogen fertilizer utilization rate of the smooth bromegrass sample, α is the final forage yield of the test field corresponding to the smooth bromegrass sample, β is the final forage yield of the test field with no nitrogen fertilizer application preset, and γ is the final nitrogen fertilizer content of the test field corresponding to the smooth bromegrass sample;

[0102] Calculating the nitrogen fertilizer productivity of the smooth bromegrass samples in the test field by using the following nitrogen fertilizer productivity formula:

[0103]

[0104] Wherein, PFP is the nitrogen fertilizer productivity of the smooth bromegrass sample, γ is the final nitrogen fertilizer content of the test field corresponding to the smooth bromegrass sample, and β is the final forage yield of the test field with no nitrogen fertilizer application preset.

[0105] Specifically, by calculating the nitrogen utilization rate of each experimental plot, the nitrogen absorption capacity of different varieties of smooth bromegrass can be obtained. By calculating the nitrogen productivity of each experimental plot, the nitrogen conversion capacity of different varieties of smooth bromegrass can be obtained. Through different experimental control groups, the nitrogen absorption capacity and nitrogen conversion capacity of different varieties of smooth bromegrass in experimental soils with different nitrogen concentrations can be obtained, so as to screen out high-quality varieties of smooth bromegrass.

[0106] S5. Determine the smooth bromegrass varieties with nitrogen utilization rate and nitrogen productivity both greater than the preset utilization rate threshold and productivity threshold in the M group of control groups as high-quality and excellent varieties.

[0107] Since the higher the nitrogen utilization rate and nitrogen productivity, the better the growth state and forage yield of smooth bromegrass, the high-quality and excellent varieties of smooth bromegrass need to be screened according to the comparison of the nitrogen utilization rate and the nitrogen productivity.

[0108] In the embodiment of the present invention, the smooth bromegrass varieties with nitrogen utilization rate and nitrogen productivity both greater than the preset utilization rate threshold and productivity threshold in the M group of control groups are determined as high-quality and excellent varieties. For example, the nitrogen utilization rate and nitrogen productivity of variety A are 65% and 70% respectively, and the preset utilization rate threshold and productivity threshold are 60% and 60% respectively. Then variety A of smooth bromegrass is a high-quality and excellent variety.

[0109] Specifically, smooth bromegrass is one of the forage grasses with the highest feeding value. Studying and screening high-quality and excellent varieties of smooth bromegrass has great value for the livestock industry. It is liked by various livestock and poultry throughout the year and is an excellent forage grass suitable for both grazing and mowing. Screening high-quality and excellent varieties through nitrogen utilization rate and nitrogen productivity is more accurate and practical, and can better meet the requirements of seed selection.

[0110] As Figure 4 shown, it is a functional module diagram of a smooth bromegrass screening device based on efficient nitrogen utilization provided by an embodiment of the present invention.

[0111] Based on the functions realized by the smooth bromegrass screening device 100 for efficient nitrogen utilization of the present invention, the smooth bromegrass screening device 100 for efficient nitrogen utilization may include a seed selection module 101, an experiment module 102, a first calculation module 103, a second calculation module 104, and a final determination module 105. The modules of the present invention can also be referred to as units.

[0112] In this embodiment, the functions of each module / unit are as follows:

[0113] The seed selection module 101: Select N seedlings of smooth bromegrass of different varieties or wild materials, and divide the N seedlings of smooth bromegrass into M groups of control experiments, with each group having N seedlings of different varieties of smooth bromegrass;

[0114] The experimental module 102: Plant M groups of control experiments into M*N*4 experimental fields with the same nutritional basis, select M different concentrations of nitrogen fertilizers, and apply them to the experimental fields of M groups of control experiments respectively;

[0115] The first calculation module 103: After a preset time period, in the M*N*4 experimental fields, use the spiral sampling method to collect P Bromus inermis samples from each experimental field, detect the soil nitrogen fertilizer content and the sample forage yield of the P Bromus inermis samples, and calculate the weighted average to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field;

[0116] The second calculation module 104: Calculate the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the Bromus inermis samples in each experimental field according to the final nitrogen fertilizer content and the final forage yield;

[0117] The final determination module 105: Determine the Bromus inermis varieties or germplasm materials with nitrogen fertilizer utilization rate and nitrogen fertilizer productivity greater than the preset utilization rate threshold and productivity threshold in the M groups of control groups as high-quality germplasms.

[0118] Specifically, each module in the Bromus inermis screening device 100 based on nitrogen efficient utilization described in the embodiments of the present invention adopts the same technical means as the Figures 1 to 3 Bromus inermis excellent germplasm screening method based on nitrogen efficient utilization described above, and can produce the same technical effects, which will not be elaborated here.

[0119] In addition, obviously, the word "including" does not exclude other units or steps, and the singular does not exclude the plural. The multiple units or devices stated in the system claims can also be implemented by one unit or device through software or hardware. The words such as first and second are used to represent names and do not represent any specific order.

[0120] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for screening excellent germplasms of Bromus inermis Leyss. based on efficient nitrogen utilization, characterized in that, The method includes: Selecting Bromus inermis seedlings of N different varieties or wild materials, dividing the N Bromus inermis seedlings into M groups of control experiments, and each group has Bromus inermis seedlings of N different varieties; Planting the M groups of control experiments into M * N * 4 experimental fields with the same nutritional basis respectively, selecting M different concentrations of nitrogen fertilizers, and applying them to the experimental fields of the M groups of control experiments respectively; After a preset time period, in the M * N * 4 experimental fields, using the spiral sampling method to collect P Bromus inermis samples from each experimental field, detecting the soil nitrogen fertilizer content of the P Bromus inermis samples and the forage yield of the samples, and calculating the weighted average to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field; Calculating the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the Bromus inermis samples in each experimental field according to the final nitrogen fertilizer content and the final forage yield; Determining the Bromus inermis varieties or germplasm materials with nitrogen fertilizer utilization rate and nitrogen fertilizer productivity greater than the preset utilization rate threshold and productivity threshold in the M groups of control groups as high-quality germplasms; The using the spiral sampling method to collect P Bromus inermis samples from each experimental field includes: Drawing a spiral in the preset experimental field model diagram according to the spiral formula; Taking the center of the experimental field as the fixed point of the spiral and calculating the arc length of the spiral; Dividing the arc length into P parts, and taking Bromus inermis samples with the same area in the experimental fields corresponding to each arc length segment; It is characterized in that, the drawing a spiral in the preset experimental field model diagram according to the spiral formula includes: Drawing a spiral in the preset experimental field model diagram using the following spiral formula: ； ； Among them, is the said spiral line, is the rotational speed of the fixed point of the spiral line in polar coordinates, is the angular velocity of the fixed point of the spiral line in polar coordinates, is the moment, is the angle of rotation of the fixed point of the spiral line; The taking the center of the experimental field as the fixed point of the spiral and calculating the arc length of the spiral includes: Calculating the arc length of the spiral using the following arc length calculation formula: ； Among them, is the arc length of the spiral line, is the rotational speed of the fixed point of the spiral line in polar coordinates, is the angular velocity of the fixed point of the spiral line in polar coordinates, is the number of half turns the spiral line has turned, is the angle by which the fixed point of the spiral line rotates.

2. The method for screening excellent Bromus inermis germplasms based on efficient nitrogen utilization according to claim 1, characterized in that, The selecting M different concentrations of nitrogen fertilizers and applying them to the experimental fields of the M groups of control experiments respectively includes: Within the preset nitrogen fertilizer concentration threshold range, selecting a nitrogen concentration value every preset interval, and a total of M are selected; applying nitrogen fertilizers to the experimental fields of the M groups of control experiments according to the M nitrogen concentration values.

3. The method for screening excellent Bromus inermis germplasm based on efficient nitrogen utilization according to any one of claims 1 to 2, characterized in that The detecting the soil nitrogen fertilizer content of the P Bromus inermis samples and the forage yield of the samples, and calculating the weighted average to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field includes: Detecting the sample soil nitrogen fertilizer content of the P Bromus inermis samples one by one according to the soil detection method; Weighing the P Bromus inermis samples, and calculating the forage yield of the samples according to the preset forage yield; Obtaining the preset nitrogen fertilizer weight of the P sample soil nitrogen fertilizer contents, calculating the nitrogen fertilizer weighted average of the nitrogen fertilizer weights, and determining the nitrogen fertilizer weighted average as the final nitrogen fertilizer content of the experimental field; Obtaining the preset weight of the P sample forage yields, calculating the weighted average of the weights, and determining the weighted average as the final forage yield of the experimental field.

4. The method for screening excellent Bromus inermis germplasms based on efficient nitrogen utilization according to claim 3, characterized in that, The obtaining the preset nitrogen fertilizer weight of the P sample soil nitrogen fertilizer contents and calculating the nitrogen fertilizer weighted average of the nitrogen fertilizer weights includes: Calculating the nitrogen fertilizer weighted average of the nitrogen fertilizer weights using the following weighted average formula: ； Among them, is the weighted average of the nitrogen fertilizers, is the number of samples, is the soil nitrogen fertilizer content of the th sample, is the currently calculated number of samples.

5. The method for screening excellent Bromus inermis germplasm based on efficient nitrogen utilization according to claim 1, wherein Calculating the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the smooth bromegrass samples in each test field according to the final nitrogen fertilizer content and the final forage yield, including: Calculating the nitrogen fertilizer utilization rate of the smooth bromegrass samples in the test field by using the following nitrogen fertilizer utilization rate formula: ； wherein is the nitrogen fertilizer utilization rate of the smooth bromegrass sample, is the final forage yield of the test field corresponding to the smooth bromegrass sample, is the final forage yield of the test field with no nitrogen fertilizer application preset, is the final nitrogen fertilizer content of the test field corresponding to the smooth bromegrass sample.

6. The method for screening excellent Bromus inermis germplasms based on efficient nitrogen utilization according to claim 1, wherein Calculating the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the smooth bromegrass samples in each test field according to the final nitrogen fertilizer content and the final forage yield, including: Calculating the nitrogen fertilizer productivity of the smooth bromegrass samples in the test field by using the following nitrogen fertilizer productivity formula: ； wherein, is the nitrogen fertilizer productivity of the smooth bromegrass sample, is the final nitrogen fertilizer content of the test field corresponding to the smooth bromegrass sample, is the final forage yield of the test field without nitrogen fertilizer application preset.

7. A Bromus inermis screening device based on efficient nitrogen utilization, which is applied to the method for screening excellent germplasm of Bromus inermis based on efficient nitrogen utilization according to any one of claims 1-6, and is characterized in that The device includes: Seed selection module: Selecting smooth bromegrass seedlings of N different varieties or wild materials, and dividing the N smooth bromegrass seedlings into M groups of control experiments, with each group having N smooth bromegrass seedlings of different varieties; Experiment module: Planting the M groups of control experiments into M*N*4 experimental fields with the same nutritional basis respectively, selecting M different concentrations of nitrogen fertilizers, and applying them to the experimental fields of the M groups of control experiments respectively; First calculation module: After a preset time period, in the M*N*4 experimental fields, using the spiral sampling method to collect P smooth bromegrass samples from each test field, detecting the soil nitrogen fertilizer content and the sample forage yield of the P smooth bromegrass samples, and calculating the weighted average to obtain the final nitrogen fertilizer content and the final forage yield of the experimental field; Second calculation module: Calculating the nitrogen fertilizer utilization rate and nitrogen fertilizer productivity of the smooth bromegrass samples in each test field according to the final nitrogen fertilizer content and the final forage yield; Final determination module: Determining the smooth bromegrass varieties or germplasm materials with nitrogen fertilizer utilization rate and nitrogen fertilizer productivity greater than the preset utilization rate threshold and productivity threshold in the M groups of control groups as high-quality germplasms.

Images