A device and method for measuring droplet flight position

Abstract

The application belongs to the technical field of ultrasonic pipetting and relates to a device and a method for measuring a droplet splashing position, comprising: a plurality of measuring chips, a data processing module and a display module; when a droplet splashes onto the measuring chip, the resistance of the measuring chip changes; the plurality of measuring chips are arranged in a rectangular array; the data processing module receives the resistance values of the measuring chips at various positions, so that the droplet splashing position is determined through the positions where the resistance values change; and the display module displays according to the droplet splashing position. The application can determine the droplet splashing position and is used for testing and calibrating the droplet splashing position in an ultrasonic pipetting system.

Description

TECHNICAL FIELD

[0001] The present application relates to a device and method for measuring the droplet splashing position, belonging to the technical field of ultrasonic pipetting. BACKGROUND

[0002] With the development of biomedical technology, the scarcity and high cost of experimental samples, the large number of experimental times, and the high cost of labor have become a prominent contradiction, and automatic micro sampling has become a necessary equipment in many biochemical laboratories. Among them, the ultrasonic micro sampling technology is an important means of micro sampling with its high precision, full automation and other characteristics.

[0003] In the ultrasonic sampling process, because it is micro sampling, and the droplets splash at a high speed, it is difficult to identify the droplet splashing position with the naked eye. Because the droplet diameter is too small, even if the droplet splashes onto the surface of the object, the static observation of the object surface with the droplet adhered, it is also difficult to observe such small droplets with the naked eye. SUMMARY

[0004] In view of the above problems, the purpose of the present application is to provide a device and method for measuring the droplet splashing position, which can determine the droplet splashing position, and is used for testing and calibrating the droplet splashing position in the ultrasonic pipetting system.

[0005] To achieve the above purpose, the present application provides the following technical scheme: a device for measuring the droplet splashing position, comprising: a plurality of measuring chips, a data processing module and a display module, when the droplet splashes onto the measuring chip, the resistance of the measuring chip changes, and a plurality of measuring chips are arranged in a rectangular array; the data processing module accepts the resistance value of each position of the measuring chip, so as to determine the droplet splashing position through the resistance value change position; the display module displays according to the droplet splashing position.

[0006] Further, the measuring chip comprises a porous water-absorbing material, when the droplet splashes onto the measuring chip, the resistance of the measuring chip changes, and after the droplet evaporates, the resistance of the measuring chip returns to normal.

[0007] Further, the measuring chip comprises two layers of conductive layers, each layer of conductive layer is tightly arranged by conductive filaments, and the arrangement directions of the conductive filaments in the two layers of conductive layers are different, when the droplet splashes onto the measuring chip, the conductive filaments in the two layers of conductive layers are connected, so as to change the conductivity of the measuring chip, and after the droplet evaporates, the measuring chip returns to the non-conductive state.

[0008] Further, when the droplet splashes onto the measuring chip, the droplet exists to cause the light flux received by the area covered by the droplet to change, and through the photoelectric effect, the change of the light flux is converted into an electric signal, so as to measure the existence or distribution of the droplet.

[0009] Further, when the droplet splashes on the measuring chip, the droplet generates pressure on the measuring chip, and the pressure is converted into an electric signal through a piezoelectric effect, so that the presence or distribution of the droplet is measured.

[0010] Further, the arrangement direction of the conductive filaments in the two conductive layers is perpendicular.

[0011] Further, the display module is an LED array or an LED pattern arranged in the same way as the measuring chip, and the LED array or the LED pattern can store the signal output by the measuring chip and can reflect the dynamic effect of the droplet distribution caused by multiple droplets in a preset time.

[0012] The application also discloses a method for measuring the droplet splashing position, which adopts the device for measuring the droplet splashing position.

[0013] Further, the measuring chip comprises a porous water-absorbing material, and when the droplet splashes on the measuring chip, the resistance of the measuring chip changes, and the resistance of the measuring chip returns to normal after the droplet evaporates.

[0014] Further, the measuring chip comprises two conductive layers, each of which is tightly arranged by conductive filaments, and the arrangement direction of the conductive filaments in the two conductive layers is perpendicular, so that the conductive filaments in the two conductive layers are connected when the droplet splashes on the measuring chip, thereby changing the conductivity of the measuring chip, and the measuring chip returns to the non-conductive state after the droplet evaporates.

[0015] The application has the following advantages due to the above technical scheme: the application can determine the droplet splashing position and is used for testing and calibrating the droplet splashing position in the ultrasonic pipetting system; the droplet splashing position which is difficult to observe with naked eyes can be obtained through the measuring chip array, and the application can be used for testing and calibrating the droplet splashing position in the ultrasonic pipetting system. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is a structural schematic view of the device for measuring the droplet splashing position in an embodiment of the application;

[0017] Figure 2 is a structural schematic view of the measuring chip matrix in an embodiment of the application;

[0018] Figure 3This is a schematic diagram of a measurement chip usage scenario in one embodiment of the present invention;

[0019] Figure 4 This is a schematic diagram of a device for measuring the position of a droplet splash in another embodiment of the present invention;

[0020] Figure 5 This is a schematic diagram of the structure of the measuring chip in one embodiment of the present invention;

[0021] Figure 6 This is a schematic diagram of the measurement chip structure in another embodiment of the present invention. Detailed Implementation

[0022] To enable those skilled in the art to better understand the technical solutions of the present invention, the present invention is described in detail through specific embodiments. However, it should be understood that the specific embodiments are provided only for a better understanding of the present invention and should not be construed as limiting the present invention. In the description of the present invention, it should be understood that the terminology used is for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0023] To address the challenge of visually identifying droplet splash locations during ultrasonic sampling, which involves micro-volume sampling and high-velocity droplet splashing, this invention discloses a device and method for measuring droplet splash locations. The device includes several measuring chips, a data processing module, and a display module. When a droplet splashes onto a measuring chip, the resistance of that chip changes. The measuring chips are arranged in a rectangular array. The data processing module receives the resistance values ​​of the measuring chips at various locations, thereby determining the droplet splash location based on the change in resistance value. The display module displays the droplet splash location. This invention can determine droplet splash locations and is used for testing and calibrating droplet splash locations in ultrasonic pipetting systems. By using a measuring chip array, it can determine the splash locations of droplets that are difficult to observe with the naked eye, making it suitable for testing and calibrating droplet splash locations in ultrasonic pipetting systems. The following detailed description, in conjunction with the accompanying drawings and embodiments, illustrates the invention.

[0024] Example 1

[0025] This embodiment provides a device for measuring the position of droplet splash, such as... Figure 1 As shown, it includes: several highly sensitive measurement chips 1, a data processing module, and a display module. When a droplet splashes onto the measurement chip 1, the electrical charge of the measurement chip 1 changes. The several measurement chips 1 are arranged in a rectangular array or an arbitrary LED pattern, such as... Figure 2 As shown, Figure 2Each small square in the diagram represents a resistor chip, which can operate independently without interfering with each other. Each measuring chip 1 can individually measure the resistance change of that chip, store the signal output by the measuring chip 1, directly provide the horizontal and vertical phase coordinates of the droplets, and reflect the dynamic effect of droplet distribution caused by multiple droplets within a preset time. In this embodiment, the measuring chip 1 is preferably a resistance measuring chip 1, but it is not limited to resistance measuring chip 1. It can also be measured using other electrical parameters or combinations of parameters, such as voltage, current, inductance, capacitance, etc., or by utilizing the photoelectric effect, piezoelectric effect, electromagnetic effect, etc., by sensing light signals, pressure signals, or magnetic field signals and converting them into electrical signals, thereby reflecting the presence information of the droplets.

[0026] like Figure 3 As shown, the jetting device controls the transducer 2 to generate ultrasonic waves through an excitation signal source, and focuses the ultrasonic waves onto the jetting outlet orifice through an acoustic lens on the dielectric substrate. Simultaneously, a pulsed voltage is applied to the orifice plate 3 where the jetting outlet orifice is located to form an electrostatic field between the orifice plate 3 and the substrate. Under the combined action of acoustic pressure and electric field force, the liquid surface rapidly deforms and forms jetting droplets. The formed jetting droplets splash onto the measuring chip 1. The resistance change of the measuring chip 1 has only two states: change and no change. The data processing module receives the resistance values ​​of the measuring chip 1 at various locations, thereby determining the droplet splash position based on the resistance value change position. The display module displays the droplet splash position. This array of measuring chips 1 can also be of other shapes. The above-described resistance change applies to voltage measuring chip 1, current measuring chip 1, inductance measuring chip 1, and capacitance measuring chip 1.

[0027] In another embodiment of this application, the measuring chip 1 can be configured such that when a droplet splashes onto the measuring chip 1, the presence of the droplet causes a change in the light flux received by the area covered by the droplet. Through the photoelectric effect, the change in light flux is converted into an electrical signal, thereby measuring the presence or distribution of the droplet. Alternatively, the measuring chip 1 can be configured such that when a droplet splashes onto the measuring chip 1, the droplet exerts pressure on the measuring chip 1. Through the piezoelectric effect, the pressure is converted into an electrical signal, thereby measuring the presence or distribution of the droplet.

[0028] The data processing module can be a computer or other device with computing capabilities, facilitating data processing and quickly obtaining the changing position of the resistor chip. If the data processing module is a computer, the display module can be the computer's built-in display module, meaning no additional display module is needed. Alternatively, if the data processing module does not have a display function, a separate display module can be provided. Figure 4As shown in the figure, the display module can be an LED array with the same arrangement as the measurement chip 1. The measurement chip 1 can also be connected to the same LED array, and the position of the liquid droplet splashing can be visually obtained by the on and off of the LED lights. The embodiment can obtain the splashing position of the liquid droplet which is difficult to observe with the naked eye through the measurement chip 1 array, and can be used for testing and calibration of the liquid droplet splashing position in the ultrasonic pipetting system.

[0029] As shown in the figure, Figure 5 The measurement chip 1 includes a porous water-absorbing material with moderate conductivity. When the liquid droplet splashes onto the measurement chip 1, the liquid droplet enters the measurement chip 1, the resistance of the measurement chip 1 changes, and after the liquid droplet evaporates, the resistance of the measurement chip 1 returns to normal. The measurement chip 1 can be cleaned and reused multiple times, is energy-saving and environmentally friendly, and can reduce the cost of raw materials. The splashed liquid droplet, even deionized water, cannot completely lose conductivity, and whether the liquid is wetted will inevitably affect the conductivity. In this embodiment, the detection of the change in conductivity of the measurement chip 1 only needs to exceed the threshold value, that is, only the change is concerned, and the size of the change in conductivity does not need to be considered.

[0030] As shown in the figure, Figure 6 The measurement chip 1 includes two layers of conductive layers arranged very close to each other. Each layer of conductive layer is tightly arranged by conductive filaments, and the arrangement direction of the conductive filaments in the two layers of conductive layers is perpendicular. The two layers of conductive layers are normally not conductive. When the liquid droplet splashes onto the measurement chip 1, the liquid droplet changes the conductivity of the measurement chip 1 due to the surface tension and capillary effect of the two layers of conductive layers. After the liquid droplet evaporates, the measurement chip 1 returns to the non-conductive state and can be reused.

[0031] Embodiment two

[0032] Based on the same inventive concept, the embodiment discloses a method for measuring the splashing position of a liquid droplet, which adopts any of the above-mentioned devices for measuring the splashing position of a liquid droplet, and includes the following steps:

[0033] S1 arrange a plurality of measurement chips 1 into a matrix, and test each chip separately;

[0034] As shown in the figure, Figure 5 The measurement chip 1 includes a porous water-absorbing material with moderate conductivity. When the liquid droplet splashes onto the measurement chip 1, the liquid droplet enters the measurement chip 1, the resistance of the measurement chip 1 changes, and after the liquid droplet evaporates, the resistance of the measurement chip 1 returns to normal. The measurement chip 1 can be cleaned and reused multiple times, is energy-saving and environmentally friendly, and can reduce the cost of raw materials. The splashed liquid droplet, even deionized water, cannot completely lose conductivity, and whether the liquid is wetted will inevitably affect the conductivity. In this embodiment, the detection of the change in conductivity of the measurement chip 1 only needs to exceed the threshold value, that is, only the change is concerned, and the size of the change in conductivity does not need to be considered.

[0035] As Figure 6 shown, the measuring chip 1 includes two layers of conductive layers close to each other, each layer of conductive layer is formed by closely arranging conductive filaments, the arrangement direction of the conductive filaments in the two layers of conductive layers is perpendicular, the two layers of conductive layers are not conductive normally, when the liquid droplet splashes to the measuring chip 1, the liquid droplet changes the conductivity of the measuring chip 1 due to the surface tension and capillary effect, the conductive filaments in the two layers of conductive layers are connected together, after the liquid droplet evaporates, the measuring chip 1 returns to the non-conductive state, and can be reused.

[0036] S2 connects the lead wire of each measuring chip 1 with the data processing module or with the display module;

[0037] S3 when the data processing module detects the resistance change of a certain measuring chip 1, judges that the position is the liquid droplet splashing position, and displays through the display module.

[0038] Finally, it should be noted that: the above examples are used to illustrate the technical solutions of the present application, but not to limit it, although the above embodiments of the present application have been described in detail, those skilled in the art should understand that: the specific embodiments of the present application can be modified or replaced, without departing from the spirit and scope of the present application, any modification or equivalent replacement, which should be covered in the protection scope of the claims of the present application. The above content is only the specific embodiments of the present application, but the protection scope of the present application is not limited to this, any person skilled in the art can easily think of changes or replacements within the technical range disclosed in the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. An apparatus for measuring a droplet flight position, characterized by, The application relates to a device for measuring the position of a liquid drop, comprising: a plurality of measuring chips, a data processing module and a display module, when a liquid drop splashes on the measuring chip, the electrical signal of the measuring chip changes, and the plurality of measuring chips are arranged in a rectangular array; the data processing module receives the electrical signal of each position of the measuring chip, so that the position of the liquid drop is determined by the change of the electrical signal; the display module displays according to the position of the liquid drop; the display module is an LED array or an LED pattern which is arranged in the same way as the measuring chip, and the LED array or the LED pattern can store the signal output by the measuring chip and can reflect the dynamic effect of the distribution of the liquid drop caused by multiple liquid drops within a preset time; the measuring chip comprises a porous water-absorbing material, when a liquid drop splashes on the measuring chip, the conductivity of the measuring chip changes, and the conductivity of the measuring chip recovers after the liquid drop evaporates; the measuring chip comprises two layers of conductive layers, each layer of the conductive layer is closely arranged by conductive filaments, and the arrangement directions of the conductive filaments in the two layers of the conductive layers are different, when a liquid drop splashes on the measuring chip, the conductive filaments in the two layers of the conductive layers are connected, so that the conductivity of the measuring chip changes, and the measuring chip returns to the non-conductive state after the liquid drop evaporates; the arrangement directions of the conductive filaments in the two layers of the conductive layers are perpendicular.

2. The apparatus for measuring a droplet flight position according to claim 1, wherein when a liquid drop splashes on the measuring chip, the light flux received by the covering area of the liquid drop changes, the change of the light flux is converted into an electrical signal through a photoelectric effect, so that the existence of the liquid drop or the distribution of the liquid drop is measured.

3. The apparatus for measuring a droplet flight position according to claim 1, wherein when a liquid drop splashes on the measuring chip, the liquid drop generates pressure on the measuring chip, the pressure is converted into an electrical signal through a piezoelectric effect, so that the existence or the distribution of the liquid drop is measured.

4. A method for measuring a droplet flight position, characterized by, The device for measuring the position of a liquid drop is used, and the device comprises the following steps: a plurality of measuring chips are arranged in a matrix or a preset pattern, and each chip is tested separately; the lead wire of each measuring chip is connected with the data processing module or the display module; when the data processing module detects the change of the electrical signal of a certain measuring chip, the position of the liquid drop is determined, and the display module displays the position of the liquid drop; the measuring chip comprises a porous water-absorbing material, when a liquid drop splashes on the measuring chip, the resistance of the measuring chip changes, and the resistance of the measuring chip recovers after the liquid drop evaporates; the measuring chip comprises two layers of conductive layers, each layer of the conductive layer is closely arranged by conductive filaments, the arrangement directions of the conductive filaments in the two layers of the conductive layers are perpendicular, when a liquid drop splashes on the measuring chip, the conductive filaments in the two layers of the conductive layers are connected, so that the conductivity of the measuring chip changes, and the measuring chip returns to the non-conductive state after the liquid drop evaporates; the arrangement directions of the conductive filaments in the two layers of the conductive layers are perpendicular.

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