Detection device and reagent bottle

Abstract

The application discloses a detection equipment and a reagent bottle, the detection equipment comprises a positioning module, the positioning module has an insertion port, and the positioning module is configured for reagent bottle insertion and locking; a heating module, the heating module is configured for heating the extraction film in the reagent bottle; a shooting module, the shooting module comprises a turntable, a probe, a plurality of filter assemblies and a sensing element, the turntable is rotatably connected to the positioning module, the probe is fixed opposite to the positioning module and opposite to the end of the insertion port, the plurality of filter assemblies are arranged at intervals around the rotation axis of the turntable, the plurality of filter assemblies are driven by the turntable to be opposite to the probe selectively, and the sensing element is configured to cooperate with the probe to take the light passing through the filter assembly. According to the detection equipment provided by the embodiment of the application, a plurality of detection modules are integrated into the same equipment, an integrated design is adopted, and fluorescent quantitative detection is conveniently realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medicine, in particular to a detection device and a reagent bottle suitable for the detection device. BACKGROUND

[0002] In the medical diagnosis industry, the detection results of reagents are often needed to diagnose the disease, such as COVID-19. For the sake of accuracy and stability, it is usually necessary to collect samples by swabs and then extract samples by reagents. The existing detection device is large in size, and needs to be in different negative pressure spaces during the detection process. Moreover, since an open detection method is adopted, the detection results are easily affected, and the surrounding environment may be polluted. SUMMARY

[0003] One object of the present application is to provide a detection device, which integrates multiple detection modules into the same device and adopts an integrated design to facilitate fluorescence quantitative detection.

[0004] The detection device according to the embodiment of the present application comprises: a positioning module having an insertion port, the positioning module being configured for reagent bottle insertion and locking; a heating module configured for heating an extraction film in the reagent bottle; a shooting module comprising a turntable, a probe, multiple filter assemblies and a sensing element, the turntable being rotatably connected to the positioning module, the probe being fixed opposite to the positioning module and opposite to the end of the insertion port, the multiple filter assemblies being arranged at intervals around the rotation axis of the turntable, the multiple filter assemblies being driven by the turntable to be opposite to the probe selectively, and the sensing element being configured to cooperate with the probe to take light passing through the filter assembly.

[0005] The detection device according to the embodiment of the present application integrates multiple detection modules into the same device and adopts an integrated design to facilitate fluorescence quantitative detection.

[0006] In addition, the detection device according to the above-mentioned embodiment of the present application can also have the following additional technical features:

[0007] Optionally, the turntable is provided with multiple power connection structures insulated from each other, the power connection structure comprising: an insulating column fixed opposite to the positioning module; a power connection ring rotatably sleeved on the insulating column; a conductive part provided on the insulating column and slidably abutting against the power connection ring, the conductive part being electrically connected with the power connection ring; and an insulating ring provided between the conductive part and the turntable to electrically isolate the conductive part from the turntable, wherein the filter assembly is electrically connected with the power connection ring.

[0008] Optionally, the insulating column is a hollow structure and is provided with a through hole on the peripheral surface, and the conductive member is a ball head plunger arranged in the insulating column and extending out of the through hole, and a ball head of the ball head plunger abuts against the conductive member.

[0009] Optionally, the rotating disc comprises a hub and a plurality of filter seats, the plurality of filter seats are connected to the hub and arranged around the hub, a plurality of filter assemblies are matched with the plurality of filter seats one by one, and opposite ends of the hub are provided with mounting grooves, and the mounting grooves are provided with the power connection structures.

[0010] Optionally, the detection device further comprises a support seat and a mounting shell, the mounting shell covers the support seat, the inductive element is arranged on the support seat, the probe is arranged on the mounting shell, the rotating disc and the plurality of filter assemblies are arranged in the mounting shell, and the insulating columns of the two power connection structures are connected to the support seat and the mounting shell respectively.

[0011] Optionally, the detection device further comprises a support seat and a mounting shell, the mounting shell covers the support seat, the inductive element is arranged on the support seat, the probe is arranged on the mounting shell, the inductive element is opposite to the probe, and the plurality of filter assemblies are driven by the rotating disc to be opposite to the probe and the inductive element selectively.

[0012] Optionally, the rotating disc further comprises an outer gear ring, the outer gear ring surrounds and is fixedly connected to the rotating disc seat and is driven to rotate by the rotating disc motor.

[0013] Optionally, the positioning module comprises a mounting seat, the mounting seat is provided with a heating device for heating a reagent bottle, the mounting seat is arranged between the rotating disc and the insertion port, the mounting seat is provided with a matching hole, and the insertion port, the matching hole and the probe are opposite along an axis of the insertion port.

[0014] Optionally, the detection device further comprises a machine shell, the positioning module, the heating module and the shooting module are arranged in the machine shell, the insertion port is exposed from an outer surface of the machine shell, and the machine shell is provided with a heat dissipation opening in communication with an internal space of the machine shell.

[0015] Optionally, the detection device further comprises a display screen, a display surface of the display screen is configured as part of an appearance surface of the positioning module.

[0016] According to the reagent bottle of the embodiment of the present application, the reagent bottle is suitable for the detection device described above, and is characterized in that: a tube body is provided with an extraction cavity for reaction and extraction, a sample cavity for sample injection, and a waste liquid cavity for waste liquid collection, the sample cavity and the waste liquid cavity are connected to the extraction cavity through micro-channels, and the extraction cavity is suitable for accommodating an extraction membrane; a tube cap is suitable for sealing the tube body; at least one of the tube cap and the tube body is provided with a sealed reagent cavity, and the tube cap is configured to be movable to pierce the reagent cavity when sealing the tube body, and the reagent cavity is configured to be suitable for reagent flowing into the sample cavity and / or the extraction cavity after being pierced, and the extraction membrane is suitable for being inserted into the positioning module and being opposite to the shooting module.

[0017] Optionally, the tube body and the tube cap are suitable for being locked by the positioning module respectively, and the positioning module is configured to be suitable for driving the tube cap to rotate relative to the tube body.

[0018] Optionally, an anti-falling groove is arranged on the outer circumferential surface of the first end of the tube body, the anti-falling groove is arranged along the circumferential direction of the tube body, and the positioning module is suitable for cooperating with the anti-falling groove to position the reagent bottle along the axial direction of the insertion port.

[0019] Optionally, a plurality of tooth grooves are arranged on the outer circumferential surface of the tube cap, the tooth grooves are parallel to the axis of the tube body, and a plurality of the tooth grooves are arranged along the circumferential direction of the tube cap at intervals, so as to be suitable for the positioning module to lock the tube cap in the circumferential direction.

[0020] Optionally, an anti-skid rib is arranged on the outer circumferential surface of the first end of the tube body, the anti-skid rib extends along the direction parallel to the axis of the tube body, and a plurality of the anti-skid ribs are arranged at intervals along the circumferential direction of the tube body.

[0021] Optionally, a positioning groove is arranged on the outer circumferential surface of the first end of the tube body, the positioning groove extends along the direction parallel to the axis of the tube body, so as to be suitable for the positioning module to lock the tube body in the circumferential direction. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 FIG. 1 is a schematic diagram of a detection device according to an embodiment of the present application.

[0023] Figure 2 FIG. 2 is a schematic diagram of a detection device according to an embodiment of the present application after removing the shell.

[0024] Figure 3 FIG. 3 is a partial schematic diagram of a detection device according to an embodiment of the present application.

[0025] Figure 4 FIG. 4 is a partial schematic diagram of a detection device according to an embodiment of the present application in another direction.

[0026] Figure 5 Figure 1 is a schematic diagram of a mounting base and positioning assembly of a detection device according to an embodiment of the present application.

[0027] Figure 6 Figure 2 is a schematic diagram of a mounting base and heating element, hot air module of a detection device according to an embodiment of the present application.

[0028] Figure 7 Figure 3 is a schematic diagram of a mounting base of a detection device according to an embodiment of the present application.

[0029] Figure 8 Figure 4 is a partial schematic diagram of a detection device according to an embodiment of the present application.

[0030] Figure 9 Figure 5 is a schematic diagram of Figure 8 Figure 6 is a schematic diagram showing the structure after removal of the mounting shell.

[0031] Figure 10 Figure 7 is a schematic diagram of a turntable, probe, filter assembly cooperation in a detection device according to an embodiment of the present application.

[0032] Figure 11 Figure 8 is a schematic diagram of a turntable, probe, filter assembly and inductive element cooperation in a detection device according to an embodiment of the present application.

[0033] Figure 12 Figure 9 is a schematic diagram of the inner side of a power connection structure of a detection device according to an embodiment of the present application.

[0034] Figure 13 Figure 10 is a schematic diagram of a reagent bottle according to an embodiment of the present application.

[0035] Figure 14 Figure 11 is a sectional view of a reagent bottle according to an embodiment of the present application.

[0036] Figure 15 Figure 12 is a schematic diagram of Figure 14 Figure 13 is a partial enlarged schematic diagram of the A area of the circle.

[0037] Figure 16 Figure 14 is a sectional view of a reagent bottle according to an embodiment of the present application.

[0038] Figure 17 Figure 15 is a schematic diagram of a septum of a reagent bottle according to an embodiment of the present application.

[0039] Figure 18 Figure 16 is a schematic diagram of a septum of a reagent bottle according to an embodiment of the present application.

[0040] Figure 19 Figure 17 is a schematic diagram of a tube body of a reagent bottle according to an embodiment of the present application.

[0041] Figure 20is a schematic view of a tube of a reagent bottle according to an embodiment of the present application.

[0042] Figure 21 is a schematic view of a tube of a reagent bottle according to an embodiment of the present application.

[0043] Figure 22 is a schematic view of an inner side of an inner end plate of a reagent bottle according to an embodiment of the present application.

[0044] Figure 23 is a schematic view of an outer side of an inner end plate of a reagent bottle according to an embodiment of the present application.

[0045] Figure 24 is a schematic view of an inner side of an outer end plate of a reagent bottle according to an embodiment of the present application. DETAILED DESCRIPTION

[0046] Embodiments of the present application are described in detail below with reference to the attached drawing figures, wherein the same or like designations indicate the same or like elements or elements having the same or similar function throughout the attached drawing figures. The embodiments described below are exemplary and are intended to be illustrative of the present application and are not to be used to limit the present application.

[0047] The detection device 200 according to an embodiment of the present application comprises a positioning module, and the positioning module is provided with an insertion opening 201 for inserting the reagent bottle 100. After the reagent bottle 100 is inserted, the reagent bottle 100 can be locked, heated, heat-sealed, and subjected to quantitative fluorescence detection, etc. After the reagent bottle is inserted into the insertion opening, the reagent bottle can be locked by the positioning module. The different operation devices of the reagent bottle 100 are described below with reference to the drawing.

[0048] In combination with Figures 1 to 7 , a device for heating the reagent bottle 100 according to an embodiment of the present application is described, which comprises a plurality of heating elements 22 and a driving device 23. The plurality of heating elements 22 are respectively movable to selectively heat the extraction membrane in the reagent bottle 100. That is, the plurality of heating elements 22 can all heat the extraction membrane in the reagent bottle 100. In general, in order to facilitate the control of the heating temperature, one or more of the plurality of heating elements 22 can be used to heat the reagent bottle 100, so as to avoid damaging the reaction reagent and to avoid fatigue and improve the service life of the detection device 200. The driving device 23 is connected with the heating elements 22 to drive the heating elements 22 to move.

[0049] The detection equipment 200 according to the embodiment of the present application can selectively heat the extraction film in the reagent bottle 100 by arranging a plurality of heating members 22, so as to meet different detection temperatures, thereby improving the application range of the detection equipment 200, and meanwhile, the detection of the same detection target under a plurality of temperature conditions can be met.

[0050] The heating member 22 in the present application can move in a plurality of directions to approach and move away from the reagent bottle 100, that is, to switch between heating and not heating the reagent bottle 100. For example, the heating member 22 can be rotated to approach and move away from the reagent bottle 100. The heating member 22 can also be arranged to move in the radial direction of the insertion hole to approach and move away from the reagent bottle 100. In addition, some embodiments of the present application are provided to heat the reagent bottle 100.

[0051] In some embodiments of the present application, the heating member 22 is movably arranged on the positioning module in a direction parallel to the axis of the insertion hole 201. That is, the heating member 22 moves in a direction parallel to the axis of the insertion hole 201 to approach and move away from the reagent bottle 100, so as to heat and not heat the reagent bottle 100. This can avoid interference between the heating member 22 and other devices (for example, the device for rotating the pipe cap, the device for locking the pipe body, etc.). The stability of the movement of the heating member 22 is improved, and the structure of the detection equipment 200 is simplified, which facilitates the design, production and maintenance of the detection equipment 200.

[0052] As Figure 5 The heating member 22 has a heating end for heating the reagent bottle 100, and the heating end is used to approach or abut against the reagent bottle 100 to heat the reagent bottle 100. The heating end is opposite to the insertion hole 201 along the axis of the insertion hole 201, and the projection of the heating ends of the plurality of heating members 22 in the direction of the axis of the insertion hole 201 is configured to have an annular shape with an observation port. Therefore, the reagent bottle 100 can be conveniently heated, and meanwhile, by arranging the observation port, the viewing of the reagent bottle 100 by the detection device (for example, the probe 272 described below) can be avoided. In the case of convenient heating, the observation can be facilitated, so as to improve the detection efficiency.

[0053] As Figure 6The driving device 23 includes a plurality of heating motors and heating cams corresponding to the plurality of heating pieces 22. The heating cam is rotatably embedded in the corresponding heating piece 22, and the heating motor is connected with the heating cam for driving the corresponding heating piece 22 to move. Specifically, the heating piece 22 can be provided with a plug-in hole, and the size of the plug-in hole can be larger than the size of the heating cam. Since the heating cam is a non-rotating body relative to the motor shaft of the heating motor, the outer periphery of the heating cam has different positions relative to the motor shaft of the heating motor, thereby achieving the driving of the heating piece 22. Of course, other structures in the prior art can also be used to achieve the driving of the heating piece 22 in the present application.

[0054] As shown in Figure 6 and Figure 7 , the positioning module includes a mounting seat 26, and the heating piece 22 and the driving device 23 are arranged on the mounting seat 26 and away from the insertion port 201. The mounting seat 26 is provided with a matching hole 203, and the heating piece 22 is adapted to extend into the matching hole 203 to heat the reagent bottle 100. By arranging the mounting seat 26, the integration of the heating device can be facilitated, the assembly and maintenance of the detection equipment 200 can be facilitated, and the stability of the movement of the heating piece 22 is ensured.

[0055] As shown in Figure 5 , the mounting seat 26 is provided with a positioning assembly opposite to the insertion port 201 and adapted to accommodate and position the end of the reagent bottle 100. In use, the reagent bottle is inserted from the insertion port 201, and the end of the reagent bottle is inserted into the positioning assembly, and the reagent bottle is positioned by the positioning assembly, so that the pipe body of the reagent bottle is fixed, and the pipe cap is conveniently rotated.

[0056] As shown in Figure 5 , the positioning assembly can include a positioning ring 211 adapted to accommodate the end of the reagent bottle 100. In use, the end of the reagent bottle is inserted into the positioning ring 211.

[0057] Optionally, as shown in Figure 5The inner circumferential surface of the positioning ring 211 is provided with a positioning rib 212 extending along the direction parallel to the axis of the insertion port 201, and the positioning rib 212 is used to position the reagent bottle 100 along the circumference of the insertion port 201. Correspondingly, the bottle body of the reagent bottle 100 is provided with a positioning groove which can be a structure matched with the positioning rib 212. During assembly, the reagent bottle 100 can be inserted into the insertion port 201, and at the same time, the positioning rib 212 is inserted into the positioning groove, so as to fix the bottle body of the reagent bottle 100. The positioning rib 212 can be arranged in an asymmetric form relative to the axis of the insertion port 201, so that the reagent bottle 100 will be inserted into the insertion port 201 along a specific orientation. For example, when performing a heat sealing process, by limiting the orientation of the reagent bottle 100, the heat sealing needle can be inserted into the reagent bottle 100 to complete the heat sealing of the corresponding opening in the reagent bottle 100.

[0058] Optionally, as Figure 5 The positioning assembly further includes a plug 213 movably connected to the mounting seat 26 along the radial direction of the positioning ring 211, and the plug 213 is used to position the reagent bottle 100 along the axial direction of the insertion port 201. Correspondingly, the end of the reagent bottle 100 can be provided with an anti-extraction groove. During use, the plug 213 can be inserted into the anti-extraction groove, so as to position the reagent bottle 100 along the axial direction of the insertion port 201, and prevent the reagent bottle 100 from being extracted from the insertion port 201.

[0059] In the present application, the reagent can be put into the reagent bottle 100 before the reagent bottle 100 is inserted into the detection device 200, and the mixing and reaction of the reagent and the sample can also be completed. Alternatively, the reagent bottle 100 can be inserted into the detection device 200, and the actual putting of the reagent can be realized through the corresponding structure on the detection device 200.

[0060] In combination Figure 3 and Figure 4 In some embodiments of the present application, the positioning module further includes a rotating barrel 214 and a driving structure 215. The insertion port 201 is constructed in the rotating barrel 214, and the rotating barrel 214 is rotatable about the axis of the insertion port 201. The driving structure 215 is connected to the rotating barrel 214 to drive the rotating barrel 214 to rotate, and the rotating barrel 214 is adapted to lock the cap of the reagent bottle 100 to drive the cap to rotate. During use, the reagent bottle 100 is inserted into the positioning module, wherein the reagent bottle 100 includes a tube body and a cap. By rotating the cap relative to the tube body, the reagent can be put into the reagent bottle 100. Through the foregoing positioning assembly, the tube body of the reagent bottle 100 can be fixed, and the cap can be locked and rotated by the rotating barrel, so that the rotation of the cap relative to the tube body can be realized, and the putting of the reagent can be completed. Therefore, by arranging the rotating barrel 214 and the driving structure 215, the automatic putting of the reagent in the reagent bottle 100 can be realized.

[0061] Optionally, the heating element 22 of the present application is a constant temperature module. Through the constant temperature module, the extraction membrane in the reagent bottle 100 can be heated to a predetermined temperature to complete the detection, improve the detection effect, and avoid fatigue caused by excessive heating speed, thereby improving the structural strength and service life.

[0062] In addition, the present application also provides some technical solutions to realize the heat sealing of the opening in the reagent bottle 100. In some embodiments of the present application, the detection device 200 further comprises a heat sealing device 24 and a heat sealing drive 25. The heat sealing device 24 is opposite to the insertion port 201 along the axial direction of the insertion port 201, and is movably arranged in the positioning module along the axial direction of the insertion port 201. The heat sealing device 24 comprises a heat sealing needle adapted to be inserted into the reagent bottle 100 to perform heat sealing. The heat sealing drive 25 is arranged on the positioning module, and the heat sealing drive 25 is connected to the heat sealing device 24 to drive the heat sealing device 24 to move. After the reagent bottle 100 is inserted into the detection device 200, the heat sealing needle can be inserted into the reagent bottle 100 by moving the heat sealing device 24, so as to complete the heat sealing of the corresponding opening in the reagent bottle 100, thereby improving the accuracy of the detection result.

[0063] Optionally, as Figure 6 , the heat sealing drive 25 comprises a heat sealing motor and a heat sealing cam. The heat sealing cam is rotatably embedded in the heat sealing device 24, and the heat sealing motor is connected to the heat sealing cam to drive the heat sealing device 24 to move. The driving mode of the heat sealing drive 25 is basically the same as that of the heating drive.

[0064] Optionally, in combination with the foregoing description, the positioning module comprises a mounting seat 26, and the heat sealing device 24 is arranged on the mounting seat 26 and away from the insertion port 201. The mounting seat 26 is provided with a matching hole 203, and the heat sealing device 24 is adapted to extend into the insertion port 201 through the matching hole 203. This facilitates the integration of various components in the detection device 200, and facilitates assembly and maintenance. In combination with the foregoing embodiments, the heat sealing device 24 and the heating element 22 are both arranged on the mounting seat 26.

[0065] As Figure 5 , in some embodiments of the present application, a limiting plate 261 is arranged in the matching hole 203. The limiting plate 261 is a hollow ring, and the limiting plate 261 is provided with a guide hole 262 corresponding to the heat sealing needle. This realizes the guidance of the heat sealing needle and improves the stability of the heat sealing effect. In addition, a guide cylinder can also be arranged on the limiting plate 261. The guide cylinder is a hollow cylindrical structure, and the heat sealing cover can pass through the guide cylinder. The heat sealing needle is guided through the guide cylinder. The guide cylinder is embedded in the heating element 22, so that the guide cylinder realizes the guidance of the heating element 22, and the guide cylinder simultaneously realizes the guidance of the heating cylinder and the heat sealing needle, thereby simplifying and optimizing the structure.

[0066] In addition, the detection device 200 of the present application further comprises a device for observing the reagent bottle 100, and through observation of the reagent bottle 100 under different temperature conditions, a reasonable and accurate conclusion can be finally given.

[0067] As Figures 8 to 12 In some embodiments of the present application, the shooting module comprises a rotating disc 271, a probe 272, a plurality of filter assemblies 273 and a sensing element 274. The rotating disc 271 is rotatably connected to the positioning module. The probe 272 is fixed opposite to the positioning module and opposite to the end of the insertion port 201. The plurality of filter assemblies 273 are arranged at intervals around the rotation axis of the rotating disc 271. The plurality of filter assemblies 273 are driven by the rotating disc 271 to be selectively opposite to the probe 272. The sensing element 274 is configured to cooperate with the probe 272 to take light after passing through the filter assembly 273. In use, different filter assemblies 273 can be used to observe the reagent bottle 100 by rotating the rotating disc 271 to meet the detection requirements and improve the accuracy of the detection results.

[0068] Among them, one sensing element 274 can be arranged on each filter assembly 273 to improve the detection efficiency, and one sensing element 274 can also be arranged to observe the form effect under different filter assemblies 273. Alternatively, in combination with Figure 10 and Figure 11 The sensing element 274 is fixed opposite to the positioning module and opposite to the probe 272. The plurality of filter assemblies 273 are driven by the rotating disc 271 to be selectively opposite to the probe 272 and the sensing element 274. By fixing the sensing element 274 to the positioning module, the wiring of the sensing element 274 can be simplified, the wire bundle can be prevented from being entangled during the rotation of the rotating disc 271, the stability of signal transmission can be effectively improved, and the number of sensing elements 274 can be reduced to reduce costs.

[0069] In combination with Figure 11 and Figure 12In some embodiments of the present invention, the turntable 271 is provided with multiple mutually insulated electrical connection structures. Each electrical connection structure includes an insulating post 281, an electrical connection ring 282, and a conductive element 283. The insulating post 281 is fixed relative to the positioning module. The electrical connection ring 282 is rotatably sleeved on the insulating post 281. The conductive element 283 is disposed on the insulating post 281 and slidably abuts against the electrical connection ring 282. The conductive element 283 is electrically connected to the electrical connection ring 282. The filter assembly 273 is electrically connected to the electrical connection ring 282. The conductive element 283 can be connected to a power source via a wiring harness. Since the insulating post 281 is fixedly connected to the positioning module, the electrical connection between the conductive element 283 and the corresponding structure on the positioning module is positioned, preventing wire entanglement. The contact ring 282 can be fixedly connected to the turntable 271. Since the conductive part 283 abuts against the contact ring 282, the two can achieve a stable electrical connection during relative movement, thereby achieving stable power connection of the conductive part 283 and connecting the filter assembly 273 to the power supply.

[0070] In addition, an insulating ring 284 can be provided between the conductive component 283 and the turntable 271 to electrically isolate the conductive component 283 and the turntable 271, thereby improving the safety of the electrical connection structure.

[0071] The insulating post 281 has a hollow structure and a through hole on its circumference. The conductive element 283 is a ball-headed plunger located inside the insulating post 281 and extending out of the through hole. The ball head of the ball-headed plunger abuts against the conductive element 283. This improves the stability of the conductive structure.

[0072] Optionally, such as Figure 11 The turntable 271 includes a hub 2711 and multiple filter holders 2712. The multiple filter holders 2712 are connected to the hub 2711 and arranged around the hub 2711. Multiple filter assemblies 273 are matched one-to-one with the multiple filter holders 2712.

[0073] In addition, the hub 2711 has mounting slots at both ends, and electrical connection structures are installed in the mounting slots.

[0074] like Figure 8 In some embodiments of the present invention, the detection device further includes a support base 208 and a mounting shell 209, with the mounting shell 209 covering the support base 208. The sensing element 274 is disposed on the support base 208, the probe 272 is disposed on the mounting shell 209, and the turntable 271 and multiple filter assemblies 273 are all disposed within the mounting shell 209. This allows for stable installation of each component, optimizing the structure and stability.

[0075] Alternatively, the insulating posts 281 of the two electrical connection structures can be connected to the support base 208 and the mounting shell 209 respectively. This achieves a stable connection between the insulating posts 281 and the positioning module.

[0076] In addition, a circular cavity can be arranged in the mounting shell 209, and the rotating disc 271 is rotatably arranged in the circular cavity. The rotating disc 271 can be positioned by the circular cavity, and stable rotation of the rotating disc 271 is guided.

[0077] Optionally, the support base 208 is provided with a rotating shaft, the rotating disc 271 comprises a rotating disc base and a rotating disc cover, the rotating disc base is rotatably connected to the rotating shaft, the rotating disc base is provided with a plurality of mounting grooves arranged around the rotating shaft and used for mounting the filter assembly 273, and the rotating disc cover covers the rotating disc base and the mounting grooves. By arranging the rotating disc base and the rotating disc cover, damage of other components to the filter assembly 273 can be avoided, the influence of the surrounding environment on the observation result is effectively excluded, and the accuracy of the observation result is improved.

[0078] Optionally, the rotating disc cover comprises a bottom plate and a plurality of partition plates, the bottom plate covers the rotating disc base, the bottom plate is provided with holes corresponding to the plurality of filter assemblies 273, the partition plates are connected to the bottom plate and extend towards the rotating disc base, and the plurality of partition plates are arranged at intervals along the circumference of the bottom plate. There is a partition plate between every adjacent two filter assemblies 273. By the bottom plate, the plurality of filter assemblies 273 can be packaged, and by the partition plates, the plurality of filter assemblies 273 can be conveniently separated, the entry of dust and other impurities into the filter assemblies 273 is reduced, and the accuracy of the detection result is improved.

[0079] As Figure 11 In some embodiments of the present application, the rotating disc 271 further comprises an outer gear ring 2713, the outer gear ring 2713 surrounds and is fixedly connected to the rotating disc base, the support base or the mounting shell is provided with a rotating disc motor, the rotating disc is driven by the rotating disc motor, and the rotating disc motor is in transmission connection with the outer gear ring 2713. Thus, the rotating disc 271 can be conveniently driven to rotate, and the stability of the rotating process of the rotating disc 271 is improved.

[0080] In combination Figures 1 to 12 In combination with the foregoing embodiments, the positioning module further comprises a mounting seat 26, the mounting seat 26 is provided with a heating device used for heating the reagent bottle 100, the mounting seat 26 is arranged between the rotating disc 271 and the insertion port 201, the mounting seat 26 is provided with a matching hole 203, and the insertion port 201, the matching hole 203 and the probe 272 are opposite along the axis of the insertion port 201. The heating device can heat the extraction membrane, so that a good detection result is obtained. Meanwhile, the mounting seat 26 is arranged between the rotating disc 271 and the insertion port 201, so that the structure of the heating device and the observation device is simplified. The matching hole 203 arranged on the mounting seat 26 can facilitate light transmission, so that observation is facilitated and the detection efficiency is improved.

[0081] In the present application, the mounting seat 26 and the support base 208 can be assembled together after being assembled respectively, so that the assembly process is simplified.

[0082] As Figure 1In some embodiments of the present application, the positioning module comprises a casing 21, and a plug-in port 201 is exposed on the outer surface of the casing 21, and the casing 21 is provided with a heat dissipation port in communication with the internal space of the casing 21. By providing the heat dissipation port, the heat dissipation of the components in the positioning module can be facilitated, and the working environment of each component in the detection device 200 can be optimized.

[0083] The positioning module, the heating module and the shooting module are all arranged in the casing.

[0084] Optionally, the detection device 200 further comprises a display screen 29, and the display surface of the display screen 29 is configured as part of the appearance surface of the positioning module.

[0085] In addition, the detection device further comprises a signal processing module arranged on the positioning module and in signal transmission with the sensing element 274; and the display screen 29 is in signal transmission with the signal processing module. By integrating the display screen 29 on the casing 21, the miniaturization and portability of the detection device 200 can be facilitated.

[0086] As can be seen from the foregoing description, the detection device 200 in the present application can be used for PCR detection, and can improve the detection efficiency of PCR and the portability of the detection device 200. The detection process can be divided into the following steps:

[0087] ①The mixing of the sample and the reagent in the reagent bottle 100 is completed, that is, the reagent is added to the cavity where the sample is located, and is extracted and collected through the extraction film. This process can be completed before the reagent bottle 100 is inserted into the detection device 200, or the reagent bottle 100 can be inserted into the detection device 200, and the detection device 200 automatically completes the process of adding the reagent. The addition of the reagent and the extraction process of the extraction film will be described in detail below;

[0088] ②After the reagent bottle 100 is inserted into the plug-in port 201, the reagent bottle 100 is locked and positioned through the structures such as the plug-in pin 213 and the positioning rib 212, so that the reagent bottle 100 can be inserted into the detection device 200 according to the predetermined trajectory and direction.

[0089] ③After the reagent bottle 100 is inserted and the reagent is added, the reagent bottle 100 can be heat-sealed to seal the mold cavity in the reagent bottle 100, and the multi-component actual storage reaction is integrally performed in the mold cavity, and the mold cavity is separated from the sample cavity and the waste liquid cavity.

[0090] ④Subsequently, the extraction membrane in the reagent bottle 100 can be heated by the heating element 22, since the extraction membrane and the outer end plate are relatively thin, the whole extraction membrane can be heated by heating one part of the extraction membrane, wherein three heating elements 22 (of course, one, two, four or more heating elements 22) can be provided to heat the reagent bottle 100, wherein the heating element 22 can be a constant temperature module, the heating element 22 can be lifted along the axis direction of the insertion port 201 to realize heating and non-heating of the reagent bottle 100, wherein the plurality of heating elements 22 are uniformly spaced around the insertion port 201, for example, the Sagen heating element 22 is arranged in a manner spaced by 120°, and the plurality of heating elements 22 can be heated by the program to heat the extraction membrane in the reagent bottle 100.

[0091] ⑤After or during heating, the reagent bottle 100 can be observed by the probe 272, the filter assembly 273 and the sensing element 274, wherein the filter assembly 273 can be switched by rotating the rotating disc 271, the filter assembly 273 can be switched by continuous one-way rotation, the wavelength switching time is shortened, and power supply can be achieved by a slip ring power supply structure. Wherein, five different color fluorescent filter assemblies 273 can be provided to improve detection accuracy.

[0092] Through the above steps, the detection of the reagent can be realized, and the detection efficiency is improved, wherein the microscopic light path is miniaturized in the present application, a high-sensitivity color camera is provided, a colorless fluorescent filter assembly 273 is adopted, and the fluorescent image of each fluorescent channel can be shot.

[0093] In combination with the foregoing detection device 200, the present application further provides a reagent bottle 100, which can be applied to the foregoing detection device 200.

[0094] As Figure 13As shown, the present application also provides a reagent bottle 100, which can be applied to the aforementioned detection device, and can also be produced, sold and used independently. The reagent bottle 100 comprises a tube body 11 and a tube cap 12, the tube body 11 is provided with an extraction cavity, a sample cavity and a waste liquid cavity, and the sample cavity and the waste liquid cavity are communicated with the extraction cavity through micro flow channels. The extraction cavity is used for reaction and extraction, the sample cavity is used for sample injection, and the waste liquid cavity is used for waste liquid collection. The tube cap 12 is suitable for closing the tube body 11. At least one of the tube cap and the tube body is provided with a closed reagent cavity, and the tube cap is configured to be movable when closing the tube body to be suitable for piercing the reagent cavity, and the reagent cavity is configured to be suitable for reagent flowing into the sample cavity and / or the extraction cavity after being pierced. Therefore, in the reagent cavity of the present application, after the tube cap closes the tube body, the injection of the reagent can still be carried out by moving the tube cap, so as to realize the closed-tube injection of the reagent, the closed-tube extraction and the closed-tube reaction. For example, in the PCR detection, the extraction and reaction of the sample are carried out in the detection tube, so as to effectively avoid the influence of the external environment on the detection process, and also can avoid the influence of the aerosol generated in the detection process on the surrounding environment.

[0095] In addition, in combination with the foregoing embodiments, after the reagent bottle 100 is inserted into the insertion port, the extraction cavity of the reagent bottle 100 can be opposite to the heating module and the shooting module, so as to realize the heating and shooting of the extraction film of the reagent bottle 100, thereby realizing effective quantitative fluorescence detection, and the process of the fluorescence quantitative detection can be completed by one device, and the detection is simplified.

[0096] In some embodiments of the present application, the tube body 11 is provided with a sleeve 111, the first end (referring to the lower end of the tube body 11 in the drawings) of the tube body 11 is provided with an extraction cavity, the sleeve 111 is configured with a sample cavity 1102, and the sleeve 111 and the tube body 11 are configured with a waste liquid cavity. The waste liquid cavity and the sample cavity are communicated with the extraction cavity through micro flow channels. The tube cap 12 covers the second end (referring to the upper end of the tube body 11 in the drawings) of the tube body 11. The sample cavity 1102 can be placed with a sample, for example, a sample swab A collected is placed into the sample cavity 1102, wherein in order to reduce the size of the reagent bottle 100, the sample swab A such as a cotton swab can be broken, and the effective part is placed in the sample cavity 1102. The tube cap 12 covers the tube body 11 and can move along the tube body 11, so that the injection of the reagent can be realized by moving the tube cap 12 along the tube body 11. Different embodiments are provided in the present application to realize the injection of the reagent, for example, the reagent is placed in the tube cap 12 and pierced by the sleeve 111; the reagent can also be placed in the tube body 11, and the injection of the reagent is realized by moving the tube cap 12. During the injection of the reagent, the tube cap 12 closes the tube body 11, which can avoid the generation of aerosol and other pollutants, avoid the pollution of the sample and the reagent in the reagent bottle, and also can avoid the pollution to the external environment. The present application will be described in detail.

[0097] The closed reagent adding mode is adopted in the application, which can prevent bottle pollution and prevent pollution to the external environment, and reduce the influence of the external environment on the reagent bottle during extraction and reaction process. Thus, moderate or room temperature storage and work can be realized.

[0098] As Figure 14 In some embodiments of the application, a closed reagent cavity 1201 is arranged in the cap 12, the cap 12 is movably connected to the tube body 11, and the tube body also has a piercing structure adapted to pierce the reagent cavity 1201 during movement of the cap 12 relative to the tube body 11, and to communicate the reagent cavity 1201 with the sleeve, so that the reagent can still be put, the sample can still be extracted, and the like when the cap 12 closes the tube body 11, and closed tube extraction can be realized. That is, during movement of the cap 12, the piercing structure in the tube body also moves relative to the cap 12 and pierces the reagent cavity 1201 in the cap 12, and after the reagent cavity 1201 in the cap 12 is pierced, the reagent cavity 1201 can be communicated with the sample cavity 1102, so that the reagent in the reagent cavity 1201 is put into the sample cavity 1102. The reagent in the reagent cavity 1201 can be a liquid reagent to facilitate reagent putting.

[0099] In the application, the reagent cavity 1201 in the cap 12 can be constructed in various ways, for example, an air bag is arranged in the cap 12, a plurality of partitions are arranged in the cap 12 to construct a plurality of reagent cavities 1201, and of course, in order to facilitate piercing and communication of the sleeve 111 with the reagent cavity 1201, an inner tube 121 can also be arranged in the cap 12 to construct the reagent cavity 1201, which will be described in detail below.

[0100] In some embodiments of the application, only one reagent cavity can be arranged in the cap, or a plurality of reagent cavities can be arranged in the cap, and the plurality of reagent cavities are adapted to be pierced by the piercing structure in sequence and connected to the sleeve in sequence. When one reagent cavity is arranged in the cap, the reagent can be quickly put after the reagent cavity is pierced, and when a plurality of reagent cavities are arranged in the cap, the actual separate and sequential putting of the plurality of reagent cavities can be realized.

[0101] The plurality of reagent cavities in the cap can be arranged in various ways to facilitate piercing by the piercing structure.

[0102] Alternatively, the cap is movably connected to the tube body along the axial direction of the tube body, and the plurality of reagent cavities are arranged in the cap in the axial direction. At this time, by moving the cap along the axial direction of the tube body, the plurality of reagent cavities can be pierced in sequence and connected to the sample cavity in sequence, and the reagent can be put.

[0103] As Figures 14 to 16The inner tube 121 is inserted into the tube body 11 from the second end of the tube body 11, and the sleeve 111 can sequentially pierce and connect the plurality of reagent cavities 1201. By arranging the plurality of reagent cavities 1201 in the inner tube 121, the reagents in the reagent cavities 1201 can be conveniently delivered. The plurality of reagent cavities 1201 are arranged along the axis of the tube body 11, so that the sleeve 111 can sequentially pierce the plurality of reagent cavities 1201 during the movement of the cap 12, thereby achieving separate delivery of the reagents in the reagent cavities 1201 and meeting different detection requirements.

[0104] Optionally, in combination with Figures 14 to 16 The end of the inner tube 121 away from the sleeve 111 is closed, and the inner tube 121 is provided with a plurality of diaphragms 122 arranged along the axis of the tube body 11. Each adjacent two diaphragms 122 and the closed end of the inner tube 121 form a reagent cavity 1201. By arranging the diaphragm 122, the piercing of the sleeve 111 is facilitated, and the sleeve 111 is further facilitated to pierce and connect the reagent cavities 1201, thereby improving the delivery efficiency of the reagents.

[0105] Further, as Figure 17 and Figure 18 The diaphragm 122 includes a hard film layer 122a and a flexible film layer 122b. The hard film layer 122a is connected to the periphery of the flexible film layer 122b, and the hard film layer 122a and the flexible film layer 122b are connected into a flat plate. The flexible film layer 122b is opposite to the sleeve 111 in the axial direction of the tube body 11, and the thickness of the flexible film layer 122b is lower than the thickness of the hard film layer 122a. In use, the flexible film layer 122b with smaller thickness is opposite to the sleeve 111, so that the sleeve 111 will easily pierce the flexible film layer 122b during the movement of the cap 12, thereby further improving the delivery efficiency of the reagents.

[0106] Optionally, the flexible film layer 122b and the hard film layer 122a are configured as a recess structure with an opening facing the sleeve 111. In use, the end of the sleeve 111 will penetrate into the recess structure, and the sleeve 111 can be guided through the recess structure to facilitate the sleeve 111 to quickly and stably pierce the flexible film layer 122b. Moreover, the recess structure can also play a certain sealing role to enable the reagents in the reagent cavities 1201 to be stably delivered into the sample cavity 1102.

[0107] Optionally, in combination with Figure 17 and Figure 18The surface of the flexible film layer 122b is provided with a plurality of strip-shaped grooves 122c which are connected together and configured in a diverging shape. By providing the strip-shaped grooves 122c, the flexible film layer 122b is more easily punctured, and the efficiency of reagent delivery is improved. In combination with Figure 17 and Figure 18 It can be seen that at least one side surface of the flexible film layer 122b is provided with the strip-shaped grooves 122c, and in addition, the outer side surface of the outermost diaphragm 122 of the inner tube is provided with a plurality of annular ribs which extend in a plurality of concentric circles centered on the sleeve.

[0108] Optionally, in combination with Figures 14 to 16 The inner tube 121 is configured as a variable-diameter tube which gradually decreases in radial dimension in the direction away from the sleeve 111, and a plurality of steps are configured on the inner circumferential surface of the inner tube 121. The diaphragm 122 is arranged in the sleeve 111 and supported on the steps on the inner circumferential surface of the inner tube 121. In other words, the inner tube 121 is configured to include a plurality of tube segments which are sequentially connected in the axial direction of the sleeve 111, and the radial dimension of the tube segment away from the sleeve 111 is smaller than the radial dimension of the tube segment close to the sleeve 111, so that a step facing the sleeve 111 is configured between the adjacent two tube segments, and the diaphragm 122 can be supported on the step. In the process of puncturing the diaphragm 122 through the sleeve 111, the step can provide support for the diaphragm 122, so that the sleeve 111 can stably puncture the diaphragm 122, thereby improving the stability of the puncturing process.

[0109] The inner tube 121 in the present application can be integrally formed with the tube cap 12, and the inner tube 121 and the tube cap 12 can also be provided as separate parts. The integral forming can effectively improve the structural strength of the tube cap 12, and also simplify the forming process, but the integral forming has higher requirements for the mold, and the cost of the mold is higher in the early stage. Therefore, the present application provides a structure in which the tube cap 12 and the inner tube 121 are assembled together after separate forming, so as to simplify the production of the tube cap 12 and reduce the cost. Specifically, Figure 14 and Figure 15 The inner end surface of the tube cap 12 is provided with an annular clamping groove, and the end portion of the inner tube 121 is provided with an annular clamping hook 1202 which is clamped with the annular clamping groove. Specifically, a rib structure can be provided on the inner end surface of the tube cap 12 to configure the annular clamping groove. The annular clamping groove can be configured in a form which gradually contracts inward in the direction away from the inner end surface of the tube cap 12, or the radial dimension of the annular clamping groove gradually decreases in the direction away from the inner end surface of the tube cap 12. A structure corresponding to the annular clamping groove can be provided on the inner shell, for example, the annular clamping hook 1202 on the inner shell is provided in a gradually outward expanding shape. Through the cooperation of the annular clamping groove and the annular clamping hook 1202, stable connection between the inner tube 121 and the tube cap 12 can be achieved, and the connection strength between the inner tube 121 and the tube cap 12 is improved.

[0110] In addition, the plurality of reagent cavities can also be arranged along the circumference of the cap, and the plurality of reagent cavities can be sequentially punctured by the puncture structure through rotation of the cap relative to the tube body.

[0111] As described above, the plurality of reagent cavities can be arranged in the cap, and the plurality of reagent cavities can respectively hold a sample preservation liquid, a lysis liquid, a first washing liquid, a second washing liquid, and an air column, which are sequentially arranged to achieve separate addition. The sample preservation liquid, the lysis liquid, the first washing liquid, the second washing liquid, and the air column are sequentially arranged in the cap along the puncture order of the puncture structure. For example, in the embodiment in which the plurality of reagent cavities are arranged along the axial direction of the tube body, the sample preservation liquid, the lysis liquid, the first washing liquid, the second washing liquid, and the air column can be sequentially arranged in the plurality of reagent cavities arranged along the axial direction of the tube body, and the reagent cavity for storing the sample preservation liquid is arranged close to the opening of the cap to facilitate the first placement of the sample preservation liquid into the sample cavity.

[0112] In the present application, the end of the sleeve can be configured as the puncture structure described above, thereby simplifying the tube body.

[0113] In combination with Figure 14 and Figure 21 In order to further facilitate the puncture of the sleeve 111 to the reagent cavity 1201, a plurality of notched grooves 1103 can be arranged along the circumference of the sleeve 111 at the end of the sleeve 111. In this way, during the process of puncturing the reagent cavity 1201 by the sleeve 111, the side of the notched groove 1103 can provide a plurality of puncture points for the sleeve 111 to improve the efficiency of puncture. In addition, the cap 12 in the present application can be arranged in the form of threaded connection with the tube body 11, and the sleeve 111 punctures the reagent cavity 1201 by rotating the cap. During this process, the rotation of the cap 12 enables the sleeve 111 to rotationally puncture the reagent cavity 1201, and by arranging the notched groove 1103, the stress exerted by the circumferential edge of the notched groove 1103 on the reagent cavity 1201 will have a good stress concentration phenomenon to achieve rapid puncture of the reagent cavity 1201.

[0114] As Figure 14In some embodiments of the present application, a sealing rib 1204 is arranged on the inner end face of the cap 12, and the sealing rib 1204 is adapted to abut against the inner periphery of the second end of the tube body 11, so as to seal the cap 12 and the tube body 11. By arranging the sealing rib 1204, when the cap 12 is moved to a predetermined position along the axial direction of the tube body 11, for example, the end of the tube body 11 abuts against the inner end face of the cap 12, the sealing rib 1204 will be in contact with the inner periphery of the tube body 11, so as to seal the cap 12 and the tube body 11, avoid the reagent in the reagent bottle 100 from leaking out, improve the accuracy of the detection result, and avoid waste.

[0115] In the present application, the cap 12 can be moved along the axial direction of the tube body 11 in a straight line, of course, other ways can also be used to realize the movement of the cap 12 relative to the tube body 11. Preferably, in an embodiment of the present application, the cap 12 is threadedly connected with the tube body 11, which not only facilitates the stable connection of the cap 12 and the tube body 11, but also realizes the movement of the cap 12 relative to the tube body 11 through threaded transmission. In addition, threaded transmission can enable the sleeve 111 to have greater force to pierce the reagent cavity 1201, improve the stability of the structure and the efficiency of reagent feeding. Figures 14 to 16 In some embodiments of the present application, the cap 12 is sleeved on the tube body 11, the inner periphery of the cap 12 is provided with a first coarse thread 1205, the outer periphery of the tube body 11 is provided with a second coarse thread 1104, and the first coarse thread 1205 and the second coarse thread 1104 are matched. Through the cooperation of the first coarse thread 1205 and the second coarse thread 1104, the stable connection of the cap 12 and the tube body 11 can be realized.

[0116] The outer periphery of the tube body 11 is also provided with a fine thread, the fine thread is matched with the second coarse thread 1104, and the first coarse thread 1205 is in contact with the fine thread when the sleeve 111 pierces the reagent cavity 1201. Through the cooperation of the first coarse thread 1205 and the second coarse thread 1104, the threaded cooperation of the cap 12 and the tube body 11 can be realized, and the fine thread arranged on the tube body 11 can enhance the resistance to rotating the cap 12, so as to give appropriate feedback to the user and remind the user. The position of the fine thread in the present application can increase the resistance of the cap 12 during the process of piercing the reagent cavity 1201 by the sleeve 111, so as to facilitate the user.

[0117] In addition, as described above, the cap 12 in the present application is provided with a plurality of reagent cavities 1201, and the fine thread can facilitate the reminding of the user. If the required reagent types are less than the actual number of reagent cavities 1201, the user can find and control in time.

[0118] In other words, during the rotation of the cap 12 in this invention, when the wall of the reagent chamber 1201 inside the cap 12 (as described above, the diaphragm 122) begins or is about to begin touching the sleeve 111, the first coarse thread 1205 will engage with the second coarse thread 1104 and the fine thread, thereby providing timely feedback to the user and making it easier for the user to understand the working status of the reagent bottle 100 so as to make adjustments.

[0119] Optionally, combined Figure 16 , Figures 22-24 The tube body 11 has an inner end plate 112 and an outer end plate 113 at its first end, forming a mold cavity between them. A first through hole 1106 is formed on the inner end plate 112, connecting the sample cavity 1102 and the mold cavity. By setting the inner end plate 112 and the outer end plate 113, the first end of the tube body 11 can be easily closed, and the structure of the mold cavity is simplified, facilitating the molding of the tube body 11. In addition, an extraction membrane 118 can be placed inside the mold cavity, and the extraction membrane 118 can be held by the inner end cap and the outer end cap, improving the structural strength and stability of the reagent bottle 100.

[0120] The first and second through holes are suitable for heat sealing by a heat sealing device. Specifically, after the reagent bottle is inserted into the insertion port, a heat sealing needle can be inserted through the outer end plate into the inner end plate to seal the first and second through holes on the inner end plate.

[0121] Furthermore, to facilitate heating and observation of the reagent bottle, the positioning module in the aforementioned embodiments can be used to lock the reagent bottle. The positioning module can lock the body and cap of the reagent bottle, and can also drive the cap to move relative to the body to facilitate reagent dispensing. For example, the positioning module can drive the cap to rotate relative to the body.

[0122] Optionally, combined Figure 13 and Figure 19 An anti-detachment groove 1107 is provided on the outer circumferential surface of the first end of the tube body 11, and the anti-detachment groove 1107 is arranged along the circumference of the tube body 11. The anti-detachment groove 1107 can be an annular shape extending along the circumference of the tube body 11, or it can be a groove extending intermittently along the circumference of the tube body 11. When the tube body 11 is placed in the testing equipment, the tube body 11 can be axially positioned by the corresponding structure on the testing equipment to prevent the reagent bottle 100 from falling out of the testing equipment.

[0123] In conjunction with the preceding embodiments, the positioning module is adapted to cooperate with the anti-dislodgement groove to position the reagent bottle axially along the insertion port, wherein the pin in the aforementioned embodiments can be inserted into the anti-dislodgement groove to lock the reagent bottle.

[0124] In addition, a anti-coming-off protrusion or the like can be arranged on the outer circumferential surface of the first end of the tube body 11, and axial positioning of the reagent bottle 100 can also be achieved by the anti-coming-off protrusion or the like.

[0125] Optionally, in combination with Figure 19 , a positioning groove 1108 can also be arranged on the outer circumferential surface of the first end of the tube body 11, and the positioning groove 1108 can extend in a direction parallel to the axis of the tube body 11. In use, a positioning rib can be arranged at a corresponding position of the detection equipment to achieve circumferential positioning of the tube body 11.

[0126] Circumferential positioning of the tube body can be achieved by the aforementioned positioning module, for example, embedding the aforementioned positioning rib into the positioning groove to achieve circumferential locking of the tube body.

[0127] Optionally, as Figure 13 , a plurality of tooth grooves 1206 are arranged on the outer circumferential surface of the cap 12, the tooth grooves 1206 are parallel to the axis of the tube body 11, and the plurality of tooth grooves 1206 are arranged at intervals in the circumferential direction of the cap 12. The plurality of tooth grooves 1206 can increase the friction of the outer circumferential surface of the cap 12, and when the cap 12 is manually or automatically driven to move along the axis of the tube body 11, the stability of the reagent bottle 100 can be improved.

[0128] The tooth grooves 1206 can be used to improve the stability of manual or motorized rotation of the cap 12, for example, the positioning module can be matched with the tooth grooves to achieve circumferential locking of the cap, and then the cap is rotated to dispense reagents.

[0129] Among them, the tooth grooves 1206 on the outer circumferential surface of the cap 12 can be arranged to extend in a direction parallel to the axis of the tube body 11.

[0130] Optionally, as Figure 13 and Figure 19 , an anti-skid rib 1109 is arranged on the outer circumferential surface of the first end of the tube body 11, the anti-skid rib 1109 extends in a direction parallel to the axis of the tube body 11, and a plurality of anti-skid ribs 1109 are arranged at intervals in the circumferential direction of the tube body 11. The anti-skid rib 1109 can increase the friction of the tube body 11, and in use, when the cap 12 is rotated, force needs to be applied to the cap 12 and the tube body 11 at the same time. Therefore, by arranging tooth grooves 1206 on the cap 12 and anti-skid ribs 1109 on the tube body 11, the rotation of the cap 12 relative to the tube body 11 can be facilitated.

[0131] Of course, other structures can also be arranged in the present application to increase the friction of the outer circumferential surface of the cap 12 and the outer circumferential surface of the tube body 11, for example, grooves, protrusions or other anti-skid structures can be arranged on the outer circumferential surface of the cap 12 and the outer circumferential surface of the tube body 11.

[0132] In addition, the extraction cavity in the application can be provided with replaceable extraction membrane, or the extraction membrane can be placed in the extraction cavity in advance to facilitate use and avoid pollution from the external environment. The extraction cavity is provided with the extraction membrane, the waste liquid cavity is communicated to the inner side of the extraction membrane, and the sample cavity is communicated to the outer side of the extraction membrane.

[0133] As described above, the reagent cavity 1201 is arranged in the cap 12 in the application, and in other embodiments of the application, a cavity can also be arranged in the tube body 11 to place reagents, or reagents can be placed in the tube body 11 and the cap 12.

[0134] As Figure 16 In some embodiments of the application, the tube body has a piston cavity 1110 and a piston rod 114, the piston cavity 1110 is communicated to the extraction cavity, and the inner end surface of the piston cavity 1110 is provided with a piercing structure; the cap 12 is adapted to press the piston rod 114 to make the reagent in the piston cavity 1110 enter the mold cavity. In the tube body 11, the piston cavity 1110 can be provided with reagents, and when the cap 12 moves in the direction parallel to the axis of the tube body 11, the cap 12 can press the piston rod 114, so that the reagent in the piston cavity 1110 enters the mold cavity.

[0135] In the piston cavity 1110, reagents such as freeze-dried powder can be placed, and the reagent can be in powder or other forms. In addition, reagents can be placed in the cap 12 and the tube body 11, so as to improve the application range of the reagent bottle 100 in the application. In actual use, the reagent in the cap 12 can be added first, and then the reagent in the piston cavity 1110 can be added. That is, when the sleeve 111 pierces all the reagent cavities 1201 in the cap 12 during the movement of the cap 12, the cap 12 drives the piston rod 114 to inject the reagent in the piston cavity 1110 into the mold cavity. Of course, the order of adding reagents in the cap 12 and the tube body 11 can be selected according to actual use.

[0136] Optionally, as Figure 16 A sealing ring 115 is arranged between the outer periphery of the piston rod 114 and the inner periphery of the piston cavity 1110, and the sealing ring 115 seals the gap between the piston rod 114 and the piston cavity 1110. The sealing ring 115 can seal the gap between the piston rod 114 and the piston cavity 1110, so as to prevent the reagent in the piston cavity 1110 from flowing out, and prevent foreign matters outside the piston cavity 1110 from entering the piston cavity 1110, thereby improving the stability of the reagent bottle 100.

[0137] The outer circumferential surface of the piston rod 114 is provided with a first sealing groove, and the sealing ring 115 is embedded in the first sealing groove and protrudes from the outer circumferential surface of the piston rod 114. Thus, the sealing ring 115 can be stably installed on the piston rod 114. In addition, the inner circumferential surface of the piston cavity 1110 is provided with a second sealing groove, and the part of the sealing ring 115 protruding from the outer circumferential surface of the piston rod 114 is adapted to be embedded in the second sealing ring 115 and adapted to slide out of the second sealing groove. In this way, before the piston rod 114 starts to move, the cooperation of the first sealing groove, the second sealing groove and the sealing ring 115 realizes the stable sealing between the piston rod 114 and the inner circumferential surface of the piston cavity 1110, and when the piston rod 114 is driven to move by the cap 12, the sealing ring 115 can be slid out of the second sealing groove to realize the stable extension and contraction of the piston rod 114. The piston rod 114 and the piston cavity 1110 are constructed in a structure similar to a syringe, which facilitates the injection of reagents into the mold cavity.

[0138] Of course, in the present application, the sealing ring 115 can also be fixed on the inner circumferential surface of the sealing cavity, and the piston rod 114 is movable relative to the sealing ring 115.

[0139] In combination Figure 14 and Figure 16 In some embodiments of the present application, the cap 12 is provided with an inner tube 121 extending along the axis of the tube body 11, and the cap 12 is adapted to drive the inner tube 121 to press against the piston rod 114. Thus, the stability of driving the piston rod 114 can be improved. In combination with the foregoing embodiments, the inner tube 121 is provided with a plurality of reagent cavities 1201, so that the inner tube 121 can press and drive the piston rod 114 while constructing a plurality of reagent cavities 1201 in the cap 12, thereby optimizing and simplifying the structure of the reagent bottle 100.

[0140] Optionally, the inner tube 121 is provided with a plurality of reagent cavities 1201 spaced along the axis of the tube body 11, and the inner tube 121 is adapted to be inserted into the tube body 11 from the second end of the tube body 11, so as to sequentially pierce and communicate the plurality of reagent cavities 1201 and make the liquid reagents in the reagent cavities 1201 enter the sample cavity 1102.

[0141] As mentioned above, in combination Figures 13 to 24The reagent bottle 100 provided in the present application is characterized in that the sleeve 111 and the tube body 11 are in a concentric column shape, the hollow structure of the sleeve 111 is configured to form the sample cavity 1102, and a partition structure is arranged in the cavity between the sleeve 111 and the tube body 11 to form the piston cavity 1110, and a piston rod 114 is arranged to be telescopically inserted into the piston cavity 1110, the sleeve 111 and the tube body 11 can be integrally formed, and the partition structure configured to form the piston cavity 1110 can also be integrally formed with the sleeve 111 and the tube body 11. A second coarse thread 1104 and a fine thread are arranged on the outer peripheral surface of the second end of the tube body 11, and the fine thread cooperates with the second coarse thread. The cap 12 is rotatably sleeved on the tube body 11, and a first coarse thread 1205 is arranged on the inner peripheral surface of the cap 12, which cooperates with the second coarse thread 1104 to realize the thread transmission between the cap 12 and the tube body 11, and the fine thread on the outer peripheral surface of the tube body 11 can provide resistance to the rotation of the cap 12. A sealing rib 1204 is arranged on the inner end surface of the cap 12, and when the tube body 11 is inserted into the top of the cap 12, the second end of the tube body 11 can cooperate with the sealing rib 1204 to improve the sealing effect. An inner tube 121 is arranged in the cap 12, the inner tube 121 is in a trumpet shape, and a plurality of diaphragms 122 are arranged in the inner tube 121, which form a plurality of reagent cavities 1201 in the inner tube 121, and different reagents can be stored in the plurality of reagent cavities 1201. In use, the sample is placed in the sleeve 111, the cap 12 is covered on the tube body 11, the cap 12 is rotated, the cap 12 is opposite to the central region of the diaphragm 122, and as the cap 12 is rotated, the diaphragm 122 will touch the sleeve 111, at this time, the first coarse thread 1205 contacts the fine thread, and further rotating the cap 12, the sleeve 111 will pierce the diaphragm 122, at this time, the reagent in the reagent cavity 1201 will enter the sleeve 111 to contact and mix with the sample in the sample cavity 1102. As the cap 12 is rotated, a plurality of reagent cavities 1201 can be pierced by the sleeve 111, so as to complete the injection of a plurality of reagents.

[0142] Meanwhile, when the cap 12 moves to a predetermined position, the cap 12 will contact the piston rod 114, at this time, the piston rod 114 will provide a reverse force to the cap 12 to slow down the movement of the cap 12, so as to facilitate the sufficient mixing of the reagent and the sample, and avoid the influence of the simultaneous mixing of a plurality of reagents on the detection effect, and the reagent can also be arranged in the piston cavity 1110, so that when the cap 12 moves to a specific position, the cap 12 can drive the piston rod 114 to inject the reagent in the piston cavity 1110 into the mold cavity.

[0143] In the present application, the mold cavity can be placed with test paper, extraction film 118, etc.

[0144] As mentioned above, the reagent can be added into the sleeve 111 in the present application, and different reagents can be added into the sleeve 111 to complete the detection. Moreover, after the reagent and the sample are mixed in the sleeve 111, the mixture can enter into the mold cavity. The reagent entering and leaving the mold cavity in some embodiments of the present application will be described below with reference to the accompanying drawings.

[0145] In combination Figure 14 , Figures 16 and Figures 22 to 24 , in some embodiments of the present application, the waste liquid cavity is formed between the sleeve 111 and the pipe body 11, and the mold cavity is communicated with the waste liquid cavity. The sample cavity 1102 is formed in the sleeve 111, and the waste liquid cavity is formed outside the sleeve 111. In use, the reagent can be added into the sample cavity 1102, and the reagent can enter into the mold cavity through the sample cavity 1102. The excess reagent can flow back into the waste liquid cavity, so as to improve the stability of the reagent bottle 100. The extraction membrane 118 (for example, for nucleic acid extraction) can be arranged in the mold cavity. The reagent and the sample can be retained in the mold cavity through the extraction membrane 118, and the excess part can enter into the waste liquid cavity, so as to avoid waste.

[0146] In some embodiments of the present application, the first end of the pipe body 11 is provided with the inner end plate 112 and the outer end plate 113, and the mold cavity is formed between the inner end plate 112 and the outer end plate 113. The first through hole 1106 and the second through hole 1113 are formed on the inner end plate 112. The first through hole 1106 is communicated with the sample cavity 1102 and the mold cavity, and the second through hole 1113 is communicated with the waste liquid cavity and the mold cavity. By arranging the first through hole 1106 and the second through hole 1113, the sample cavity 1102 and the waste liquid cavity can be communicated with the mold cavity, the reagent can flow in the sample cavity 1102, the mold cavity and the waste liquid cavity, the extraction of the sample can be completed, and the purpose of detecting the sample can be achieved.

[0147] Optionally, as Figure 22 and Figure 23 , the inner end plate 112 is provided with a plurality of first through holes 1106 and a plurality of second through holes 1113. The plurality of first through holes 1106 are arranged along the circumference of the inner end plate 112, and the plurality of second through holes 1113 are arranged along the circumference of the inner end plate 112. In this way, the efficiency of the reagent entering the mold cavity and contacting the extraction membrane 118 in the mold cavity can be improved, and the excess reagent can flow back into the waste liquid cavity.

[0148] Further, the distance between the first through hole 1106 and the sample cavity 1102 is greater than the distance between the second through hole 1113 and the sample cavity 1102. At this time, after the reagent enters the mold cavity, it will flow back to the waste liquid cavity after passing through the extraction membrane 118, so as to avoid the reagent flowing back to the waste liquid cavity directly through the mold cavity, and provide sufficient reaction time for the reagent and the sample.

[0149] In combination Figure 16 and Figure 21 In some embodiments of the present application, the first end of the tube body 11 is provided with an annular plate 116, the inner periphery of the annular plate 116 is along the connecting sleeve 111, the outer periphery of the annular plate 116 is along the connecting tube body 11, the outer end surface of the annular plate 116 is provided with a plurality of first guide grooves 1119, the plurality of first guide grooves 1119 are all communicated with the sample cavity 1102 and extend in a diverging manner away from the sample cavity 1102, and the first guide grooves 1119 are communicated with the first through hole 1106. Thus, the time and distance of the reagent flowing in the process of entering and discharging the mold cavity can be increased, more reaction time is provided for the reagent and the sample, and the extraction effect is enhanced.

[0150] Optionally, in combination Figure 16 and Figure 21 The annular plate 116 is provided with a third through hole 1114, the third through hole 1114 is spaced apart from the first guide groove 1119, and the third through hole 1114 is opposite to the second through hole 1113. The second through hole 1113 cooperates with the third through hole 1114 to connect the mold cavity and the waste liquid cavity. The mold cavity and the waste liquid cavity are conveniently connected, excessive pressure of the reagent in the mold cavity is avoided, and subsequent other reagents can quickly enter the mold cavity.

[0151] Optionally, as Figure 23 The outer side surface of the inner end plate 112 is provided with a containing groove 1115, and the outer end plate 113 covers the containing groove 1115 to construct the mold cavity. The bottom surface of the containing groove 1115 is provided with longitudinally and transversely intersecting flow-through grooves 1116, and the second through hole 1113 connects the flow-through grooves 1116. The extraction membrane 118 and the like can be conveniently placed in the mold cavity, and the coverage of the reagent in the mold cavity is further increased to facilitate the extraction of the extraction membrane 118.

[0152] In combination Figure 23 It can be seen that the containing groove 1115 is in a circular groove shape, the bottom surface of the containing groove is provided with a circular flow-through groove 1116, the flow-through groove 1116 is provided with a plurality of annular ribs, the plurality of annular ribs are arranged in a spaced-apart manner from inside to outside, and the plurality of annular ribs have a plurality of radial grooves for conduction. Thus, the reagent can uniformly flow to the extraction membrane in the mold cavity.

[0153] As Figure 24 In some embodiments of the present application, the inner side surface of the outer end plate 113 is provided with a center groove 1117 and a second guide groove 1118. The center groove 1117 constructs part of the mold cavity, the second guide groove 1118 is communicated with the center groove 1117 and extends in a diverging manner away from the center groove 1117, and the second guide groove 1118 is communicated with the first through hole 1106.

[0154] The reagent bottle 100 according to the embodiment of the application has a structure of multiple layers of membranes 122 separating reagents, and is suitable for detection reactions requiring reagents to be added in batches. For example, nucleic acid detection of the new coronavirus.

[0155] There are various modes in specific technical solutions: (1) the multi-layer design shown in the figure, the rotating cap 12 is used to add reagents by gradually piercing; (2) different reagents are sealed in the same plane, and the piercing structure is designed at the edge position, the first reagent is pierced by the downward displacement of the cap 12, and the piercing of other reagent cavities 1201 can be achieved by rotating the cap 12 to gradually pierce the reagent separation membranes 122. Figure 14

[0156] The reagent bottle 100 in the application can be provided with a rotating scale on the bottle body to prompt the type of reagent to be added.

[0157] The application stores multiple-component reagents in a reaction-integrated tube, and each component is stored in a container and separated by a “splitting sheet” (which can be a film or a sheet) to avoid cross-contamination. The position of each component reagent in the tube is set according to experimental needs. The number of reagent components is set according to experimental needs. After the sample is placed in the sleeve 111, the cap is screwed on. During use, the sleeve 111 pierces the membranes 122 of different components reagents one by one by screwing or pressing to displace the sleeve 111, so that different components reagents flow into the sleeve 111 in sequence, pass through the sample, and then enter the mold cavity for reaction. The reagent components that do not need to flow through the sample can flow into the mold cavity through a separate micro-channel. The mold cavity is connected to the sample cavity 1102 and the waste liquid cavity through a micro-pipe. The micro-pipe has multiple groups (multiple first through holes 1106 and multiple second through holes 1113), which can achieve large particle filtration and prevent blockage of a certain component.

[0158] An extraction membrane 118 can be provided in the mold cavity. The extraction membrane 118 can be a sheet (the optimized height is 0.2 mm), which has a large heat transfer area and uniform heat conduction, thereby shortening the reaction time of PCR. For particulate samples, the focal plane of the microscopic image is stable. The cap 12 is designed with a step corresponding to the liquid level of each reagent component, and when the cap reaches this position, the resistance increases, which serves as a position reminder during manual operation. The outer circumferential surface of the tube body 11 can be printed with a position line of the reagent components, which serves as a position reminder during manual operation.

[0159] The reagent bottle 100 in the application can be operated manually or on a machine.

[0160] ​The pipe body 11 and the pipe cap 12 in the application have a sealing design, and the pipe body 11 and the pipe cap 12 are sealed after the last kind of component reagent flows into the sleeve 111, all reagents are sealed into the pipe to avoid leakage. The waste liquid cavity can be filled with water absorption materials (filter paper or sponge, etc.), and when the waste liquid enters the waste liquid cavity, it is adsorbed by the water absorption material to prevent backflow into the micro-pipe.

[0161] In the application, any auxiliary tool is not required, and nucleic acid extraction is realized.

[0162] The PCR reagent bottle 100 in the application focuses on the structure of the liquid flow channel and the small chamber for dispensing enzyme and eluent, and the PCR reaction chamber. It is suitable for directly adding extracted nucleic acid. The dispensing enzyme in each reagent cavity 1201 in the application adopts a freeze-drying method, and each reagent cavity 1201 in the application can be respectively provided with a sample storage liquid, a lysis liquid, a washing liquid 1, a washing liquid 2, an eluent, and an enzyme reaction system. The mold cavity in the application can serve as a PCR reaction chamber, and the reaction liquid can enter the mold cavity from the sample cavity 1102 or enter the waste liquid cavity from the mold cavity.

[0163] The reaction can be promoted by heating the extraction membrane in the mold cavity at different temperatures, and detection can be realized by using different filters.

[0164] In addition, the application also provides a detection system, which comprises a positioning module, a heating module, a shooting module, and a reagent bottle. The reagent bottle is the reagent bottle according to the foregoing embodiments, wherein the positioning module has an insertion port, the first end of the reagent bottle is adapted to be inserted into the insertion port and positioned by the positioning module, the heating module is used for heating the extraction cavity, and the shooting module is opposite to the end face of the first end of the reagent bottle to be adapted to obtain a fluorescent quantitative image.

[0165] According to the detection system of the embodiment of the application, the addition of various reagents can be realized in a closed tube, and after the addition of various reagents, the reaction and nucleic acid extraction in the closed tube can be realized. In addition, the reagent bottle can also serve as a reaction chamber. In the process of obtaining a fluorescent quantitative image, the extraction membrane does not need to be taken out, but can be directly obtained and reacted, so that the detection precision is effectively improved, the pollution to the external environment is avoided, and the influence of the external environment on the detection process is avoided.

[0166] Although the embodiments of the application have been shown and described above, it should be understood that the above embodiments are exemplary and should not be construed as limiting the application. Those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the application.

Claims

1. A detection device, characterized by The detection device comprises: a positioning module having an insertion opening, the positioning module being configured for reagent bottle insertion and locking; a heating module configured for heating an extraction membrane in the reagent bottle; a shooting module comprising a rotating disc, a probe, a plurality of filter assemblies and a sensing element, the rotating disc being rotatably connected to the positioning module, the probe being fixed opposite to the positioning module and opposite to the end of the insertion opening, the plurality of filter assemblies being arranged at intervals around the rotation axis of the rotating disc, the plurality of filter assemblies being driven by the rotating disc to be selectively opposite to the probe, the sensing element being configured to cooperate with the probe to capture light passing through the filter assembly, the positioning module comprises a mounting seat, the mounting seat being provided with a heating device for heating the reagent bottle, the mounting seat being arranged between the rotating disc and the insertion opening, the mounting seat being provided with a matching hole, the insertion opening, the matching hole and the probe being opposite along the axis of the insertion opening, the rotating disc is provided with a plurality of electrically connected structures which are insulated from each other, the electrically connected structure comprising: an insulating column fixed opposite to the positioning module; an electrically connected ring rotatably sleeved on the insulating column; a conductive part provided on the insulating column and slidably abutting against the electrically connected ring, the conductive part being electrically connected with the electrically connected ring; an insulating ring arranged between the conductive part and the rotating disc to electrically isolate the conductive part and the rotating disc, wherein the filter assembly is electrically connected with the electrically connected ring, the insulating column is a hollow structure and is provided with a through hole on the peripheral surface, the conductive part is a ball head plunger arranged in the insulating column and extending out of the through hole, and the ball head of the ball head plunger abuts against the conductive part.

2. The detection device of claim 1, wherein, The rotating disc comprises a hub and a plurality of filter seats, the plurality of filter seats being connected to the hub and arranged around the hub, the plurality of filter assemblies being correspondingly matched with the plurality of filter seats, the opposite ends of the hub are provided with mounting grooves, and the electrically connected structures are mounted in the mounting grooves.

3. The detection device of claim 2, wherein, The detection device further comprises: a support seat; a mounting shell covering the support seat, wherein the sensing element is arranged on the support seat, the probe is arranged on the mounting shell, the rotating disc and the plurality of filter assemblies are arranged in the mounting shell, and the insulating columns of the two electrically connected structures are respectively connected to the support seat and the mounting shell.

4. The detection device of claim 1, wherein, The detection device further comprises a support seat and a mounting shell, the mounting shell covering the support seat, the sensing element being arranged on the support seat, the probe being arranged on the mounting shell, the sensing element being opposite to the probe, the plurality of filter assemblies being driven by the rotating disc to be selectively opposite to the probe and the sensing element.

5. The detection device of claim 4, wherein, The rotating disc further comprises an outer gear ring, the outer gear ring surrounding and fixedly connected to the rotating disc seat and being driven to rotate by a rotating disc motor.

6. The detection device of claim 1, wherein, The detection device further comprises a machine shell, the positioning module, the heating module and the shooting module being arranged in the machine shell, the insertion opening being exposed from the outer surface of the machine shell, and the machine shell being provided with a heat dissipation opening communicating with the internal space of the machine shell.

7. The detection device of claim 6, wherein, The detection device further comprises a display screen, a display surface of the display screen being configured as a part of an appearance surface of the positioning module.

8. A reagent bottle suitable for use in the detection device according to any one of claims 1 to 7, characterized in that The detection device comprises: a tube body, the tube body being provided with an extraction cavity for reaction and extraction, a sample cavity for sample injection, and a waste liquid cavity for waste liquid collection, the sample cavity and the waste liquid cavity being communicated with the extraction cavity through micro flow channels, and the extraction cavity being adapted to accommodate an extraction membrane; a tube cap, the tube cap being adapted to seal the tube body; wherein at least one of the tube cap and the tube body is provided with a closed reagent cavity, the tube cap being configured to be movable to pierce the reagent cavity when sealing the tube body, and the reagent cavity being configured to allow reagent to flow into the sample cavity and / or the extraction cavity after being pierced, and the extraction membrane being adapted to be inserted into the positioning module and opposite to the shooting module.

9. The reagent bottle of claim 8, wherein, The tube body and the tube cap are adapted to be locked by the positioning module respectively, and the positioning module is configured to drive the tube cap to rotate relative to the tube body.

10. The reagent bottle of claim 8, wherein, An anti-falling groove is provided on an outer peripheral surface of a first end of the tube body, the anti-falling groove being arranged along a circumferential direction of the tube body, and the positioning module is adapted to cooperate with the anti-falling groove to position the reagent bottle along an axial direction of the insertion opening.

11. The reagent bottle of claim 8, wherein, A plurality of tooth grooves are provided on an outer peripheral surface of the tube cap, the tooth grooves being parallel to an axis of the tube body and being arranged along a circumferential direction of the tube cap at intervals, so as to be adapted to be locked by the positioning module in the circumferential direction.

12. The reagent bottle of claim 8, wherein, An anti-skid rib is provided on an outer peripheral surface of a first end of the tube body, the anti-skid rib extending along a direction parallel to an axis of the tube body, and a plurality of anti-skid ribs are arranged along a circumferential direction of the tube body at intervals.

13. The reagent bottle of claim 12, wherein, A positioning groove is provided on an outer peripheral surface of a first end of the tube body, the positioning groove extending along a direction parallel to an axis of the tube body, so as to be adapted to be locked by the positioning module in the circumferential direction.

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