Use of a fungicide composition containing tetramycin and quinoline copper for preventing and controlling plant Xanthomonas diseases

Abstract

The invention belongs to the field of pesticide fungicides, and discloses a use of a fungicide composition containing tetramycin and quinoline copper for preventing and controlling plant Xanthomonas diseases; the active ingredients of the fungicide composition include active ingredient A tetramycin and active ingredient B quinoline copper. The fungicide composition of the invention can be used to prevent and control various diseases on plants, has good prevention and control effects, and has the characteristics of a wide fungicide spectrum, low dosage, obvious synergistic effect, etc.

Description

Technical Field

[0001] The invention relates to the field of pesticide fungicides, and in particular to use of a fungicide composition containing tetramycin and quinoline copper for preventing and controlling plant Xanthomonas diseases. Background Art

[0002] Prokaryotic diseases of plants mainly occur in higher angiosperms. Whether it is field crops or fruit trees and vegetables, there are one or several bacterial diseases. There are more bacterial diseases in crops of Gramineae, Leguminosae and Solanaceae, and some crops may have more than three bacterial diseases. Plant pathogenic bacteria generally invade host plants through wounds and natural openings (such as water holes, stomata, and lenticels). After plants are infected by prokaryotes, the main symptoms are necrosis, rot, wilting and deformity, with less discoloration and frequent bacterial pus overflow.

[0003] Tetramycin, also known as wuningmycin, is a fermentation metabolite of Streptomyces ahygroscopicus wuzhouensis subusp l1371, which has high inhibitory activity against ascomycetes, basidiomycetes, and deuteromycetes. Tetramycin has systemic antibacterial activity, preventing the invasion and spread of pathogens. In addition, tetramycin not only has a strong antibacterial effect, but also can enhance the disease resistance of plants by inducing the increase of defense enzyme activity.

[0004] Copper8-quinolinolate is a chelated organic copper broad-spectrum fungicide. It forms a dense protective film on the surface of crops to block the contact between pathogens and crops, preventing pathogens from infecting crops. It can also act on the inside of pathogens to inhibit the activity and conduction of the main transmitters of pathogens, thereby killing pathogens. Copper8-quinolinolate has a preventive and therapeutic effect on fungal and bacterial diseases, and is suitable for the prevention and control of various diseases on fruit trees, vegetables, melons, tobacco and other crops.

[0005] Prior art CN104982446A discloses a fungicide composition containing tetramycin and quinoline copper, and discloses that the fungicide composition is used to prevent and control rice and wheat sheath blight and cucumber and grape downy mildew, etc. However, there is no relevant report on the use of tetramycin and quinoline copper for preventing and controlling plant Xanthomonas diseases. Summary of the invention

[0006] Based on the above situation, the purpose of the present invention is to provide a fungicide composition containing tetramycin and quinoline copper for preventing and controlling plant Xanthomonas diseases. The fungicide composition has good prevention and control effects, and has the characteristics of a wide fungicide spectrum, low dosage, and obvious synergistic effect.

[0007] In order to achieve the above object, a fungicide composition containing tetramycin and quinoline copper is used for preventing and controlling plant Xanthomonas diseases, wherein the active ingredients of the fungicide composition include active ingredient A tetramycin and active ingredient B quinoline copper; the mass ratio of tetramycin to quinoline copper is 1:1 to 1:100;

[0008] Furthermore, the mass ratio of the active ingredient tetramycin to quinoline copper is 1:30 to 1:90;

[0009] Furthermore, the mass ratio of tetramycin to quinoline copper is 1:49 to 1:89;

[0010] Furthermore, the mass ratio of tetramycin to quinoline copper is 1:49 to 1:79;

[0011] Furthermore, the mass ratio of tetramycin to quinoline copper is 1:49, 1:59, 1:69, 1:79;

[0012] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 5 to 90 wt%,

[0013] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 10 to 50 wt%,

[0014] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 20 to 50 wt%;

[0015] Furthermore, the formulation of the bactericidal composition may be any one of emulsifiable concentrate, aqueous emulsion, microemulsion, suspension, wettable powder or water dispersible granules;

[0016] Furthermore, the dosage form of the bactericidal composition is a suspension;

[0017] Furthermore, the suspension concentrate comprises, by weight percentage, 0.01% to 10% tetramycin, 1% to 80% quinoline copper, and the rest is 1% to 20% agriculturally acceptable wetting and dispersing agent, 0.1% to 2% thickener, 1% to 10% antifreeze agent, 0.01% to 0.3% preservative, 0.1% to 5% defoaming agent, and deionized water to 100%;

[0018] Further, the wetting agent and dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0019] The thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0020] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0021] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0022] The defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C 8 -C 10 One or more of fatty alcohols; and / or

[0023] Plant prokaryotes include Phylum Gracilicutes, Phylum Firmicutes, and Phylum Tenericutes; among them, Phylum Gracilicutes includes Agrobacterium, Erwinia, Pseudomonas, Xanthomonas, and Xylella; Firmicutes includes Clavibacter and Streptomyces; and Phylum Tenericutes includes Spiroplasma and Phytoplasma.

[0024] Furthermore, the plant pathogenic prokaryotes include: Agrobacterium, Clavibacter, Erwinia, Pseudomonas, Xanthomonas, Streptomyces;

[0025] Furthermore, the plant diseases caused by the Xanthomonas genus include: sugarcane white stripe disease [Xanthomonas albilineans (Ashby) Dowson], banana necrotic stripe disease [Xanthomonas arboricolapv.Celebensis (Gaumann) Vauterin et al.], pepper leaf spot disease [Xanthomonas axonopodis pv.Betlicola (Patel et al.) Vauterin et al.], cassava bacterial wilt disease [Xanthomonasaxonopodis pv.Manihotis (Bondar) Vauterin et al.], sugarcane gummosis [Xanthomonasaxonopodis pv.vasculorum (Cobb) Vauterin et al.], mango black spot disease [Xanthomonascampestris pv.mangiferaeindicae (Patel et al.) Robbs et al.], citrus canker disease [Xanthomonas campestris pv.Citri(Hasse)Vauterin et al.], bacterial angular leaf spot of cotton[Xanthomonas campestris pv.Musacearum(Yirgou et Bradbury)Dye], bacterial wilt of banana[Xanthomonas campestris pv.Musacearum(Yirgou et Bradbury)Dye], black rot of cruciferous vegetables[Xanthomonas campestris pv.campestris(Pammel)Dowson], scab of pepper[Xanthomonas campestris pv.Vesicatoria], bacterial leaf spot of cassava[Xanthomonas cassavae(ex Wiehe et Dowson)Vauterin et al.], angular leaf spot of strawberry[Xanthomonas fragariae Kennedyet King], yellow rot of hyacinth[Xanthomonas hyacinthi(Wakker)Vauterin et al.], bacterial leaf blight of rice[Xanthomonas oryzae pv.Oryzae(Ishiyama)Swings et al.], Xanthomonas oryzae pv.Oryzicola(Fang et al.)Swings et al.], poplar bacterial canker [Xanthomonas populi (ex Ride) Ride et Ride];.

[0026] Furthermore, the Xanthomonas is Xanthomonas campestris;

[0027] Furthermore, the plant diseases caused by the Xanthomonas genus include: mango black spot [Xanthomonas campestris pv. mangiferae indicae (Patel et al.) Robbs et al.], citrus canker [Xanthomonas campestris pv. Citri (Hasse) Vauterin et al.], cotton bacterial angular spot [Xanthomonas campestris pv. Musacearum (Yirgou et Bradbury) Dye], banana bacterial wilt [Xanthomonas campestris pv. Musacearum (Yirgou et Bradbury) Dye], cruciferous vegetable black rot [Xanthomonas campestris pv. campestris (Pammel) Dowson], pepper scab [Xanthomonas campestris pv. Vesicatoria];

[0028] Furthermore, the plant diseases caused by Xanthomonas campestris are mango black spot [Xanthomonas campestris pv. mangiferae indicae (Patel et al.) Robbs et al.] and citrus canker [Xanthomonas campestris pv. Citri (Hasse) Vauterin et al.];

[0029] Mango bacterial black spot pathogen mainly harms mango leaves, branches, flowers and fruits, producing ulcer spots, causing leaf and fruit fall and branch dieback. After the leaves are infected, polygonal or irregular dark brown spots limited by leaf veins appear; branches and flower spikes after infection appear dark brown ulcer spots, sometimes cracking longitudinally and gumming; after the fruit is infected, black star-shaped cracking spots appear, and gumming flows.

[0030] Citrus canker pathogens mainly harm citrus leaves, branches and fruits. Severe damage to seedlings and young trees will cause leaf fall and branch dieback, affecting tree vigor; severely damaged fruits will fall off, while mildly damaged fruits will have scars and be unable to withstand storage and rot, greatly reducing the commercial value of the fruits, increasing the cost of pest control for fruit farmers and damaging their economic benefits.

[0031] A use of a fungicide composition containing tetramycin and quinoline copper for preventing and controlling plant Xanthomonas diseases, wherein the fungicide composition is applied to the disease to be controlled or its growth medium at an effective dose;

[0032] Furthermore, the effective dose of the bactericidal composition is 147-189 g.ai / hm 2 .

[0033] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0034] 1) By compounding tetramycin with quinoline copper, the fungicidal activity is increased, and it can effectively prevent and control plant Xanthomonas diseases;

[0035] 2) It is safe and environmentally friendly, has broad development prospects, reduces the use of pesticides, and reduces agricultural costs;

[0036] 3) It is ultra-efficient and has a broad bactericidal spectrum, and can delay the development of drug resistance in harmful bacteria and prolong the effectiveness of the drug. DETAILED DESCRIPTION

[0037] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0038] Indoor toxicity assay

[0039] Examples Reference is made to the Guidelines for Indoor Bioassay Tests for Pesticides, Part 16: Turbidimetric Method for Inhibition of Bacterial Growth, NY / T 1156.16-2008; Part 6: Determination of Combined Effects of Mixtures, NY / T 1156.6-2006.

[0040] Example 1

[0041] Toxicity Determination of Tetramycin and Quinoline Copper against Bacterial Black Spot of Mango

[0042] Test pathogen: mango bacterial black spot (Xanthomonas campestrispv. mangiferaeindicae), provided by the group's research and development center.

[0043] Test reagents: 15% tetramycin stock drug, 98% quinoline copper technical drug, all of which are provided by the group's R&D center.

[0044] The mango bacterial black spot pathogen was transferred to nutrient agar medium (NA) and placed in a constant temperature light incubator at 28°C for continuous dark culture for 24 hours. Then the mango bacterial black spot pathogen grown on NA medium was transferred to NB medium for shaking culture for 12 hours and diluted with sterile water to 1×10 7 Prepare a spore suspension with a concentration of spores / mL for later use.

[0045] Preparation of drug mother solution: prepare 1×10 tetramycin and quinoline copper 4 mg / L mother solution; tetramycin and quinoline copper were mixed in the following ratios: tetramycin: quinoline copper = 1:1, 1:30, 1:49, 1:55, 1:60, 1:65, 1:70, 1:75, 1:89, 1:100, and each ratio was also prepared into 1×10 4 Mix the mother solution at 500 mg / L for later use.

[0046] Dosage setting: Each single dose and mixed doses of different proportions are treated with 5 series of mass concentrations according to the content of active ingredients. The specific treatment doses are as follows:

[0047] Tetramycin (A): 2, 1, 0.5, 0.25, 0.125 mg / L;

[0048] Quinoline copper (B): 320, 160, 80, 40, 20 mg / L;

[0049] A:B(1:1): 4, 2, 1, 0.5, 0.25mg / L;

[0050] A:B(1:30): 40, 20, 10, 5, 2.5mg / L;

[0051] A:B(1:49): 40, 20, 10, 5, 2.5mg / L;

[0052] A:B(1:55): 40, 20, 10, 5, 2.5mg / L;

[0053] A:B(1:60): 40, 20, 10, 5, 2.5mg / L;

[0054] A:B(1:65): 40, 20, 10, 5, 2.5mg / L;

[0055] A:B(1:70): 60, 30, 15, 7.5, 3.75mg / L;

[0056] A:B(1:75): 60, 30, 15, 7.5, 3.75mg / L;

[0057] A:B(1:89): 80, 40, 20, 10, 5mg / L;

[0058] A:B(1:100): 100, 50, 25, 12.5, 6.25mg / L;

[0059] Preparation of the drug: dilute the tetramycin mother solution to 20, 10, 5, 2.5, and 1.25 mg / L; dilute the quinoline copper mother solution to 3200, 1600, 800, 400, and 200 mg / L; dilute the mother solution of tetramycin and quinoline copper in a 1:1 ratio to 40, 20, 10, 5, and 2.5 mg / L; dilute the mother solution of tetramycin and quinoline copper in a 1:30, 1:49, 1:55, 1:60, and 1:65 ratio to 400, 200, and 100 , 50, 25 mg / L; dilute the mother liquor of tetramycin and quinoline copper in a ratio of 1:70, 1:75 to 600, 300, 150, 75, 37.5 mg / L; dilute the mother liquor of tetramycin and quinoline copper in a ratio of 1:89 to 800, 400, 200, 100, 50 mg / L; dilute the mother liquor of tetramycin and quinoline copper in a ratio of 1:89, 1:100 to 1000, 500, 250, 125, 62.5 mg / L.

[0060] Four Erlenmeyer flasks were used to treat each concentration of the test agent, with one Erlenmeyer flask for each replicate, for a total of four replicates, and an aqueous solution without the agent was used as a blank control.

[0061] The mango bacterial black spot pathogen that had been transferred, activated and cultured on NA medium for 24 hours was treated with the drug according to the above dosage setting.

[0062] Under aseptic operation conditions, in a pre-sterilized 100mL Erlenmeyer flask containing 45mL NB culture medium, quantitatively pipette 5mL of each treatment solution with a concentration of 10 times the designed concentration from low concentration to high concentration, add it to the above Erlenmeyer flask, and shake it thoroughly. The experiment set up a blank control with an aqueous solution without drug, and each treatment was repeated 4 times. Then, the pre-prepared 1×10 7 The mango bacterial black spot pathogen with a concentration of spores / mL was inoculated into a triangular flask, and 100ul of the bacterial solution was inoculated into each flask. After thorough mixing, 10mL of the solution was taken out from each treatment and placed in a test tube for later use. The triangular flask was then sealed and placed at 28°C for continuous shaking culture (120r / min).

[0063] Investigation method, time and frequency: Measure 10 ml of the mixed solution taken from each treatment and record the turbidity of each treatment; after a certain period of cultivation, when the control treatment reaches the exponential growth phase, measure and record the turbidity of each treatment.

[0064] Data processing: Based on the survey results, the bacterial growth inhibition rate of each treatment concentration on the test target was calculated according to formulas (1) and (2).

[0065] The experimental results were calculated using IBM SPSS Statistics 20 data processing software, and the toxicity regression equations, EC 50 and 95% confidence limits and R 2 .

[0066] A=A 1 -A 2······ (1)

[0067] Where:

[0068] A represents the increase in turbidity of bacterial solution;

[0069] A 1 Indicates the turbidity of bacterial solution after culture;

[0070] A 2 Indicates the turbidity of bacterial solution before culture.

[0071] P=[(A 0 -At) / A 0 ]×100······(2)

[0072] Where:

[0073] P represents growth inhibition rate;

[0074] A 0 Indicates the turbidity increase of the blank control;

[0075] At represents the increase in turbidity after chemical treatment.

[0076] The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method, and the synergistic effect of drug mixing was evaluated based on the co-toxicity coefficient (CTC), that is, CTC≤80 was antagonistic effect, 80<CTC<120 was additive effect, and CTC≥120 was synergistic effect.

[0077] The co-toxicity coefficient (CTC) is calculated according to formulas (3), (4) and (5).

[0078] TI=(S / T)×100······(3)

[0079] Where:

[0080] TI represents the measured toxicity index of the test agent;

[0081] S represents the EC of the standard agent 50 (mg / L);

[0082] T represents the EC of the test agent 50 (mg / L).

[0083] TTI=TI A ×P A +TI B ×P B······ (4)

[0084] Where:

[0085] TTI represents the theoretical toxicity index of the mixture;

[0086] TI A It represents the toxicity index of agent A;

[0087] P A Indicates the percentage of agent A in the mixture (%);

[0088] TI B It represents the toxicity index of agent B;

[0089] P B Indicates the percentage (%) of agent B in the mixture.

[0090] CTC=(ATI / TTI)×100······(5)

[0091] Where:

[0092] CTC stands for co-toxicity coefficient;

[0093] ATI means the measured toxicity index of the mixture;

[0094] TTI stands for Theoretical Toxicity Index.

[0095] Results and Analysis:

[0096] It can be seen from Table 1 that tetramycin and quinoline copper alone and their combined agents have good fungicidal activity against mango bacterial black spot pathogen. 50 The value is 0.402mg / L, single dose EC of quinoline copper 50 The value is 71.536 mg / L.

[0097] The mass ratio of tetramycin to quinoline copper was 1:30-1:100, but no antagonism was observed. The mass ratio of tetramycin to quinoline copper was 1:49-1:89, but synergistic effect was observed. When the mass ratio of tetramycin to quinoline copper was 1:70, the EC 50 It is 13.973 mg / L, the co-toxicity coefficient is 146.598, and the synergistic effect is obvious.

[0098] Table 1 Indoor combined toxicity results of different ratios of tetramycin and quinoline copper against bacterial black spot of mango

[0099]

[0100]

[0101] Example 2

[0102] Toxicity Determination of Tetramycin and Copper Quinoline against Citrus Canker

[0103] Test pathogen: Citrus canker (Xanthomonas campestris pv. Citri), provided by the Group's R&D Center.

[0104] Test reagents: 15% tetramycin stock drug, 98% quinoline copper technical drug, all of which are provided by the group's R&D center.

[0105] The citrus canker bacteria were transferred to nutrient agar medium (NA) and placed in a constant temperature light incubator at 28°C for continuous dark culture for 24 hours. The citrus canker bacteria grown on NA medium were then transferred to NB medium for shaking culture for 12 hours and diluted with sterile water to 1×10 7 Prepare a spore suspension with a concentration of spores / ml for later use.

[0106] Preparation of drug mother solution: prepare 1×10 tetramycin and quinoline copper 4 mg / L mother solution; tetramycin and quinoline copper were mixed in the following ratios: tetramycin: quinoline copper = 1:49, 1:59, 1:69, 1:79, 1:89, and each ratio was also prepared into 1×10 4 Mix the mother solution at 500 mg / L for later use.

[0107] Dosage setting: Each single dose and mixed doses of different proportions are treated with 5 series of mass concentrations according to the content of active ingredients. The specific treatment doses are as follows:

[0108] Tetramycin (A): 2, 1, 0.5, 0.25, 0.125 mg / L;

[0109] Quinoline copper (B): 320, 160, 80, 40, 20 mg / L;

[0110] A:B(1:49): 60, 30, 15, 7.5, 3.75mg / L;

[0111] A:B(1:59): 60, 30, 15, 7.5, 3.75mg / L;

[0112] A:B(1:69): 80, 40, 20, 10, 5mg / L;

[0113] A:B(1:79): 80, 40, 20, 10, 5mg / L;

[0114] A:B(1:89): 80, 40, 20, 10, 5mg / L;

[0115] Preparation of the drug: dilute the tetramycin mother solution to 20, 10, 5, 2.5, and 1.25 mg / L; dilute the quinoline copper mother solution to 3200, 1600, 800, 400, and 200 mg / L; dilute the mother solution of tetramycin and quinoline copper in a ratio of 1:49 and 1:59 to 600, 300, 150, 75, and 37.5 mg / L; dilute the mother solution of tetramycin and quinoline copper in a ratio of 1:69, 1:79, and 1:89 to 800, 400, 200, 100, and 50 mg / L.

[0116] Four Erlenmeyer flasks were used to treat each concentration of the test agent, with one Erlenmeyer flask for each replicate, for a total of four replicates, and an aqueous solution without the agent was used as a blank control.

[0117] The citrus canker bacteria that had been transferred, activated and cultured on NA medium for 24 hours were treated with the drug according to the above-mentioned dosage settings.

[0118] Under aseptic operation conditions, in a pre-sterilized 100mL Erlenmeyer flask containing 45mL NB culture medium, quantitatively pipette 5mL of each treatment solution with a concentration of 10 times the designed concentration from low concentration to high concentration, add it to the above Erlenmeyer flask, and shake it thoroughly. The experiment set up a blank control with an aqueous solution without drug, and each treatment was repeated 4 times. Then, the pre-prepared 1×10 7 The bacterial solution of citrus canker pathogen at a concentration of spores / mL was inoculated into a triangular flask, with 100ul of bacterial solution inoculated into each flask. After thorough mixing, 10mL of the bacterial solution was taken out from each treatment and placed in a test tube for later use. The triangular flask was then sealed and placed at 28°C for continuous shaking culture (120r / min).

[0119] Investigation method, time and frequency: Measure 10 mL of the mixed solution taken from each treatment and record the turbidity of each treatment; after a certain period of cultivation, when the control treatment reaches the exponential growth phase, measure and record the turbidity of each treatment.

[0120] Data processing: According to the survey results, the bacterial growth inhibition rate of each treatment concentration on the test target was calculated according to formulas (1) and (2). IBM SPSS Statistics 20 data processing software was used to calculate the experimental results, and the toxicity regression equations, EC 50 and 95% confidence limits and R 2 .

[0121] A=A 1 -A 2 ······(1)

[0122] Where:

[0123] A represents the increase in turbidity of bacterial solution;

[0124] A 1 Indicates the turbidity of bacterial solution after culture;

[0125] A 2 Indicates the turbidity of bacterial solution before culture.

[0126] P=[(A 0 -At) / A 0 ]×100······(2)

[0127] Where:

[0128] P represents growth inhibition rate;

[0129] A 0 Indicates the turbidity increase of the blank control;

[0130] At represents the increase in turbidity after chemical treatment.

[0131] The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method, and the synergistic effect of drug mixing was evaluated based on the co-toxicity coefficient (CTC), that is, CTC≤80 was antagonistic effect, 80<CTC<120 was additive effect, and CTC≥120 was synergistic effect.

[0132] The co-toxicity coefficient (CTC) is calculated according to formulas (3), (4) and (5).

[0133] TI=(S / T)×100······(3)

[0134] Where:

[0135] TI represents the measured toxicity index of the test agent;

[0136] S represents the EC of the standard agent 50 (mg / L);

[0137] T represents the EC of the test agent 50 (mg / L).

[0138] TTI=TI A ×P A +TI B ×P B ······(4)

[0139] Where:

[0140] TTI represents the theoretical toxicity index of the mixture;

[0141] TIA It represents the toxicity index of agent A;

[0142] P A Indicates the percentage of agent A in the mixture (%);

[0143] TI B It represents the toxicity index of agent B;

[0144] P B Indicates the percentage (%) of agent B in the mixture.

[0145] CTC=(ATI / TTI)×100······(5)

[0146] Where:

[0147] CTC stands for co-toxicity coefficient;

[0148] ATI means the measured toxicity index of the mixture;

[0149] TTI stands for Theoretical Toxicity Index.

[0150] Results and Analysis:

[0151] It can be seen from Table 2 that tetramycin and quinoline copper alone and their combined agents have good fungicidal activity against citrus canker. 50 The value is 0.485mg / L, single dose EC of quinoline copper 50 The value is 79.886 mg / L.

[0152] The mass ratio of tetramycin to quinoline copper was 1:49-1:89, which showed no antagonistic effect. The mass ratio of tetramycin to quinoline copper was 1:49-1:79, which showed synergistic effect. When the mass ratio of tetramycin to quinoline copper was 1:69, EC 50 It is 17.485 mg / L, the co-toxicity coefficient is 136.842, and the synergistic effect is obvious.

[0153] Table 2 Indoor combined toxicity results of different ratios of tetramycin and quinoline copper against citrus canker pathogen

[0154]

[0155] Specific preparation example:

[0156] Preparation Example 1: 35wt% tetramycin·quinoline copper suspension (1:69)

[0157] Formula: tetramycin 0.5%, quinoline copper 34.5%, polyether 3%, phenethylphenol polyether phosphate ester salt 2.5%, Guerbet alcohol polyoxyethylene ether (xp-70) 2%, magnesium aluminum silicate 0.9%, xanthan gum 0.1%, propylene glycol 3%, glycerol 1%, benzisothiazolinone 0.02%, dimethyl silicone oil 0.4%, deionized water to make up;

[0158] Preparation method: Add tetramycin and additives (except preservatives and thickeners) to the feeding kettle, start shearing to dissolve all the additives. Add quinoline copper under high shear stirring, shear evenly and then sand grind, transfer to a homogenizing kettle after sand grinding, add preservatives and thickeners, add remaining water to make up the balance, shear, homogenize and mix to obtain the corresponding product.

[0159] Preparation Example 2: 36wt% tetramycin·quinoline copper suspension (1:44)

[0160] Formula: 0.8% tetramycin, 35.2% quinoline copper, 3% polyether, 3.5% phenethylphenol polyether phosphate, 1.2% Guerbet alcohol polyoxyethylene ether, 0.8% magnesium aluminum silicate, 0.2% xanthan gum, 5% propylene glycol, 0.02% benzisothiazolinone, 0.35% dimethyl silicone oil, supplemented with deionized water

[0161] Preparation method: Same as Preparation Example 1.

[0162] Preparation Example 3: 42.6wt% tetramycin·quinoline copper suspension (1:70)

[0163] Formula: Tetramycin 0.6%, quinoline copper 42%, alkyl sulfonate 0.5%, polycarboxylate 0.5%, phenethylphenol polyether phosphate ester 3%, isomeric tridecyl alcohol polyoxyethylene ether 2%, magnesium aluminum silicate 1%, xanthan gum 0.2%, propylene glycol 5%, benzoic acid 0.1%, dimethyl silicone oil 0.35%, deionized water to make up;

[0164] Preparation method: Same as Preparation Example 1.

[0165] Preparation Example 4: 33wt% tetramycin·quinoline copper water dispersible granules (1:65)

[0166] Formula: 0.5% tetramycin, 32.5% quinoline copper, 5% lignin sulfonate, 7.5% sodium dodecylbenzene sulfonate, 8% aluminum chloride, and white carbon black as the remainder.

[0167] Preparation method: According to the formula ratio of the embodiment, the active ingredients tetramycin and quinoline copper are added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow pulverization, 10-25% of water is added, and then kneading, granulation, drying, and sieving are performed to obtain a water-dispersible granule product.

[0168] Preparation Example 5: 40wt% tetramycin·quinoline copper water dispersible granules (1:39)

[0169] Formula: 1% tetramycin, 39% quinoline copper, 5% lignin sulfonate, 7.5% sodium dodecylbenzene sulfonate, 4% aluminum chloride, 10% bentonite, and white carbon black as the balance.

[0170] Preparation method: Same as Preparation Example 4.

[0171] Example 3

[0172] Field trials to control bacterial black spot in mango

[0173] Tested crops: mango;

[0174] Test subjects: bacterial black spot;

[0175] Test agent: 35% tetramycin·quinoline copper suspension;

[0176] Control drugs: 40% quinoline copper suspension, 0.3% tetramycin aqueous solution;

[0177] Drug treatment and dosage:

[0178] Table 3 Experimental design of test drugs

[0179]

[0180]

[0181] The experiment was conducted at the mango planting base in Guangzhou, with open field cultivation, above-average fertilizer and water management, and medium soil fertility. The mangoes grew well, and no fungicide was sprayed from the time the plants were planted to the first application of the drug.

[0182] The bacterial black spot disease has caused serious damage in the experimental areas over the years. The soil, cultivation, water and fertilizer management conditions in each experimental area are consistent.

[0183] Experimental treatments: Each treatment was arranged in random blocks, with buffer rows set between adjacent plots, repeated 4 times, and 3 mango trees.

[0184] Application time: Application was carried out during the tender shoot stage, twice on April 18 and April 28, 2020. The maximum daily temperature during the test was 31°C and the minimum daily temperature was 14°C.

[0185] Investigation time: On May 23, 2020, when the condition of the blank control plot was stable, the condition of the mangoes in each treatment was investigated.

[0186] Investigation method: At the beginning of the experiment, only a few leaves showed bacterial black spot spots, so the disease base was not investigated. During the experimental investigation, two plants were investigated in each plot, and samples were taken from five points of east, west, south, north, and center. All leaves of two shoots were investigated at each point, and the total number of leaves investigated and the number of diseased leaves at each level were recorded.

[0187] Leaf Grading Method:

[0188] Level 0: no disease;

[0189] Level 1: There are 1 to 5 lesions on each leaf;

[0190] Level 3: There are 6 to 10 lesions on each leaf;

[0191] Level 5: There are 11 to 15 lesions on each leaf;

[0192] Level 7: There are 16 to 20 lesions on each leaf;

[0193] Level 9: There are more than 21 spots on each leaf.

[0194] Survey time and frequency: Investigate the disease base before applying the pesticide, and investigate the prevention and control effect 25 days after the last application of the pesticide.

[0195] Calculation method of drug efficacy:

[0196] Disease index = disease index (%) = [Σ(number of diseased plants at each level × relative level value) / (total number of plants investigated × 9)] × 100

[0197] Control effect (%) = [(disease index of control area - disease index of treated area) / disease index of control area] × 100

[0198] During the experiment, it was observed that mangoes in each treatment plot grew well, and no phytotoxicity was observed in any treatment.

[0199] The test results of each treatment agent for controlling mango bacterial black spot are shown in the following table:

[0200] Table 4 Field test results of different tested agents on mango bacterial black spot

[0201]

[0202]

[0203] Note: The protective effect (%) in the above table is the average value of each replicate. Lowercase letters represent significant differences at the 5% level, and uppercase letters represent significant differences at the 1% level.

[0204] The results showed that the tested agent 35% tetramycin·quinoline copper SC had a good control effect on mango bacterial black spot disease, and the control efficiencies of the three treatments were 90.48%, 90.98% and 92.17% respectively.

[0205] The tested pesticide 35% tetramycin·quinoline copper suspension had no phytotoxic effect on mangoes, and no other effects of the tested pesticide on the test plants were found during the observation period.

[0206] Example 4

[0207] Field trials to control citrus canker

[0208] Standard: Field efficacy test guidelines (II) Part 103: Fungicides for the control of citrus canker GB / T17980.103-2004 Test crop: Citrus

[0209] Test subjects: ulcer disease

[0210] Test location: Guilin, Guangxi

[0211] Experimental time: April 12 and April 26, 2020, with two applications. At the beginning of the experiment, sporadic ulcer spots were visible on the leaves of the plants. The weather was good during the experiment, with the highest daily temperature of 26°C and the lowest temperature of 8°C.

[0212] Experimental site: The soil fertility of the experimental site is moderate, the terrain is flat, the fertility is uniform, and the irrigation conditions are good. The cultivation conditions (soil type, fertilization, tillage, row spacing, etc.) of all experimental plots are uniform and consistent with the local agricultural cultivation practice (GAP).

[0213] Experimental treatment: Each treatment was arranged in random blocks, with buffer rows set between adjacent plots, repeated 4 times, and 3 citrus trees in each plot.

[0214] Survey method: Two plants were surveyed in each plot, and samples were taken from five points of each plant, namely, east, west, south, north and center. Ten fruits and all leaves on two shoots were surveyed at each point.

[0215] Leaf (fruit) grading method:

[0216] Level 0: no disease;

[0217] Level 1: There are 1 to 5 lesions on each leaf (fruit);

[0218] Level 3: There are 6 to 10 lesions on each leaf (fruit);

[0219] Level 5: There are 11 to 15 lesions on each leaf (fruit);

[0220] Level 7: There are 16 to 20 lesions on each leaf (fruit);

[0221] Level 9: There are more than 21 spots on each leaf (fruit).

[0222] Survey time and frequency: Investigate the disease base before applying the pesticide, and investigate the prevention and control effect 20 days after the last application of the pesticide.

[0223] Calculation method of drug efficacy:

[0224] Disease index = disease index (%) = [Σ(number of diseased plants at each level × relative level value) / (total number of plants investigated × 9)] × 100

[0225] Control effect (%) = [(disease index of control area - disease index of treated area) / disease index of control area] × 100

[0226] During the experiment, citrus fruits in each treatment plot grew well and no pesticide damage was observed in any treatment.

[0227] Results of the experiment on the control of citrus canker with various treatment agents:

[0228] Table 5 Field test results of different tested agents on citrus canker

[0229]

[0230] Note: The protective effect (%) in the above table is the average value of each replicate. Lowercase letters represent significant differences at the 5% level, and uppercase letters represent significant differences at the 1% level.

[0231] The results showed that the tested agent 35% tetramycin·quinoline copper SC had a good control effect on mango bacterial black spot disease, and the control efficiencies of the three treatments were 90.13%, 90.65% and 92.94% respectively.

[0232] Example 5

[0233] 35% tetramycin·quinoline copper suspension (0.5%+34.5%) for controlling plant Xanthomonas diseases

[0234] Field test results:

[0235] Table 6 Field test results of 35% tetramycin·quinoline copper suspension (0.5%+34.5%) for controlling plant Xanthomonas diseases

[0236]

[0237]

[0238] Through indoor toxicity determination and field tests, the pesticide composition prepared by combining tetramycin and quinoline copper according to the present invention exhibits a good control effect on plant Xanthomonas diseases.

[0239] The pesticide composition or its preparation obtained by compounding the present invention has significant prevention effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging the duration of effect. In addition, no compound agent was found to cause phytotoxicity to crops in the test, indicating that the obtained pesticide composition or preparation can reduce production cost and use cost and is safe for crops when the bactericidal synergistic effect is improved.

[0240] Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A fungicidal composition containing tetramycin and quinoline copper for preventing and controlling plant Xanthomonas diseases, characterized in that: The active ingredients of the bactericidal composition include tetramycin and quinoline copper, and the mass ratio of tetramycin to quinoline copper is 1:49-1:

79.

2. The use according to claim 1, characterized in that: The mass ratios of tetramycin and quinoline copper are 1:49, 1:59, 1:69 and 1:

79.

3. The use according to claim 1, characterized in that: Based on the total weight of the bactericidal composition being 100 wt %, the sum of the contents of tetramycin and quinoline copper in the bactericidal composition is 5 to 90 wt %.

4. The use according to claim 3, characterized in that: Based on the total weight of the bactericidal composition being 100wt%, the sum of the contents of tetramycin and quinoline copper in the bactericidal composition is 10-50wt%.

5. The use according to claim 4, characterized in that: Based on the total weight of the bactericidal composition being 100 wt %, the sum of the contents of tetramycin and quinoline copper in the bactericidal composition is 20-50 wt %.

6. The use according to claim 1, characterized in that: The dosage form of the bactericidal composition is any one of emulsifiable concentrate, aqueous emulsion, microemulsion, suspension, wettable powder or water dispersible granule.

7. The use according to claim 6, characterized in that: The dosage form of the bactericidal composition is a suspension.

8. The use according to claim 7, characterized in that: The suspending agent comprises, by weight percentage, 0.01% to 10% tetramycin, 1% to 80% quinoline copper, and the rest is 1% to 20% of an agriculturally acceptable wetting and dispersing agent, 0.1% to 2% of a thickener, 1% to 10% of an antifreeze agent, 0.01% to 0.3% of a preservative, 0.1% to 5% of a defoaming agent, and deionized water to make up to 100%.

9. The use according to claim 1, characterized in that: The plant Xanthomonas is Xanthomonas campestris ( Xanthomonas campestris).

10. The use according to claim 9, characterized in that: The plant diseases caused by Xanthomonas rape include: mango black spot disease [ Xanthomonas campestris pv. mangiferaeindicae (Patel et al.) Robbs et al.], citrus canker [ Xanthomonas campestris pv. Citri (Hasse) Vauterin et al.], cotton bacterial angular leaf spot [ Xanthomonas campestris pv.Musacearum (Yirgou et Bradbury) Dye], banana bacterial wilt [ Xanthomonas campestris pv. Musacearum (Yirgou et Bradbury) Dye], black rot of cruciferous vegetables [ Xanthomonas campestris pv.campestris (Pammel) Dowson], pepper scab[ Xanthomonas campestris pv.Vesicatoria ].

11. The use according to claim 10, characterized in that: The plant diseases caused by Xanthomonas rapeseed are mango black spot disease and citrus canker disease.

12. The use according to claim 1, characterized in that The fungicide composition is applied to the disease to be controlled or its growth medium at an effective dose.

13. The use according to claim 12, characterized in that The effective dose of the bactericidal composition is 147-189 g.ai / hm 2 .

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