A flow tube and method of use thereof

Abstract

The application discloses a cell experiment instrument, and aims to provide a flow pipe and a use method thereof, and the technical scheme is as follows: the flow pipe comprises a pipe body and a cover body which is threadedly connected with the pipe body, and an adjusting ring pipe for adjusting air permeability is arranged between the cover body and the pipe body, the adjusting ring pipe comprises a hollow ring pipe which is provided with a plurality of air permeation holes and air permeability adjusting particles which are arranged in the hollow ring pipe, the air permeability adjusting particles are configured to continue air permeation through the gaps between the particles when not under pressure, and the gaps between the particles are closed when under pressure, so that the air permeation holes of the hollow ring pipe are closed. Compared with the existing flow pipe, the flow pipe and the use method thereof can realize air permeability without leakage, and can realize three states, namely, a completely air-permeable state, a semi-air-permeable state and a sealed state. The application is suitable for the technical field of cell experiments.

Description

Technical Field

[0001] This invention relates to a cell experiment apparatus, and more specifically, to a flow cytometer and its method of use. Background Technology

[0002] Flow cytometer tubes are cell containers specifically designed for flow cytometry, available in 5ml and 14ml sizes. They are widely used in flow cytometry experiments such as detection and sorting.

[0003] Existing flow cytometry tubes have some drawbacks in use: While they are directly connected to the tube body via a cap, ensuring a tight seal, achieving permeability requires unscrewing or loosening the cap. Loosening the cap can easily cause liquid leakage, affecting experimental results. Flow cytometry tubes themselves cannot achieve permeability without leakage. Summary of the Invention

[0004] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a flow meter and its usage method.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a flow tube and its method of use, comprising a tube body and a cover body threadedly connected to the tube body, wherein an adjusting ring tube for adjusting air permeability is provided between the cover body and the tube body, the adjusting ring tube comprising a hollow ring tube having a plurality of air permeability holes and air permeability adjusting particles disposed within the hollow ring tube, the air permeability adjusting particles being configured to allow air to continue to permeate through the gaps between the particles when not under pressure; and to close the gaps between the particles when under pressure, thereby closing the air permeability holes of the hollow ring tube.

[0006] The present invention is further configured such that: a pressure plate structure is provided between the cover and the tube, the pressure plate structure includes an upper pressure plate connected to the cover and a lower pressure plate connected to the end face of the tube, each of the air permeability regulating particles constitutes an adjustable air permeable layer, and the air permeability regulating particles are arranged such that when not under pressure, they can continue to allow air to pass through; when under pressure, they can close the air vents of the hollow ring tube.

[0007] The present invention is further configured such that: the upper and lower pressure plates are also provided with a breathable structure, the breathable structure including a breathable channel provided on the upper pressure plate, an embedded groove provided on the lower side of the breathable channel, and a sealing ball provided on the upper side of the breathable channel and adapted to the embedded groove; the upper pressure plate is provided with an elastic structure for fitting the sealing ball to the embedded groove after being subjected to force, and opening the breathable channel when not subjected to force.

[0008] The present invention is further configured such that: the elastic structure includes a movable pressure plate disposed inside the upper pressure plate and slidably connected to the upper pressure plate, an elastic support member disposed between the movable pressure plate and the upper pressure plate, and a force-bearing body disposed on the movable pressure plate; the force-bearing body presses down on the sealing ball to seal the inner groove when the cover is tightened and pressed down, and when the pressing force is lost, it is subjected to the reverse force of the elastic support member to achieve a reset.

[0009] The present invention is further configured such that the structure of the lower pressure plate is the same as that of the upper pressure plate, and the upper and lower pressure plates are mirror images of each other.

[0010] A method for using a flow cytometer includes the following steps:

[0011] S1, Fully ventilated stage: Twist the cover and the tube so that the lower pressure plate on the tube and the adjusting ring tube structure inside the cover are just in contact, forming a fully ventilated state between the cover and the tube.

[0012] S2, Semi-permeable stage: Twist the cover and the tube body again to squeeze the lower pressure plate on the tube body against the adjusting ring tube structure inside the cover body. Press the cover body to ensure that the cover body is still in a state where it can move axially, forming a semi-permeable state between the cover body and the tube body.

[0013] S3, Sealing state: Twist the cover and the tube body again to press the upper and lower pressure plates, press the cover body to ensure that the cover body is in a state where it cannot move axially, thus forming a sealing state between the cover body and the tube body.

[0014] The beneficial effects of this invention are:

[0015] 1. Compared with existing flow tubes, the flow tube of the present invention and its method of use can achieve air permeability without leakage, and can achieve three states: fully permeable state, semi-permeable state and sealed state.

[0016] 2. The present invention provides an adjusting ring tube, which includes a hollow ring tube with a plurality of vent holes and ventilating adjusting particles disposed within the hollow ring tube. The ventilating adjusting particles are configured to allow air to continue to pass through the gaps between the particles when not under pressure; and to close the gaps between the particles when under pressure, thereby sealing the vent holes of the hollow ring tube and ensuring airtightness.

[0017] 3. By setting upper and lower pressure plates, the three states of the present invention can be further realized in conjunction with the adjusting ring pipe. While achieving air permeability, the possibility of leakage that may occur in the prior art will not occur.

[0018] 4. This invention has a simple structure, is easy to manufacture and operate, and is suitable for widespread implementation and application. Attached Figure Description

[0019] Figure 1 This is a structural diagram of the flow meter and its usage method according to the present invention.

[0020] Figure 2 This is a structural diagram of the pressure plate of the flow tube and its usage method of the present invention.

[0021] Figure 1-2 Reference numerals: 1. Tube body; 2. Cover body; 3. Adjusting ring tube; 4. Hollow ring tube; 5. Air permeability adjusting particles; 6. Upper pressure plate; 7. Lower pressure plate; 8. Air permeability channel; 9. Embedded groove; 10. Sealing ball; 11. Movable pressure plate; 12. Elastic support; 13. Force-bearing body. Detailed Implementation

[0022] Reference Figure 1-2 The present invention provides further explanation of embodiments of the flow meter and its usage.

[0023] For ease of explanation, spatial relative terms such as “up,” “down,” “left,” and “right” are used in the embodiments to describe the relationship of one element or feature shown in the figures relative to another element or feature. It should be understood that, in addition to the orientations shown in the figures, spatial terms are intended to include different orientations of the device in use or operation. For example, if the device in the figures is inverted, an element described as being “down” of other elements or features would be positioned “up” of those other elements or features. Therefore, the exemplary term “down” can encompass both up and down orientations. The device may be positioned in other ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0024] Moreover, relational terms such as “first” and “second” are used merely to distinguish one component from another that has the same name, without necessarily requiring or implying any such actual relationship or order between the components.

[0025] Figures 1 to 2The present invention discloses a flow meter and its method of use, comprising a pipe body 1 and a cap 2 threadedly connected to the pipe body 1. An adjusting ring pipe 3 for adjusting air permeability is provided between the cap 2 and the pipe body 1. The adjusting ring pipe 3 includes a hollow ring pipe 4 with a plurality of air vents and air permeability adjusting particles 5 disposed within the hollow ring pipe 4. The air permeability adjusting particles 5 are configured to allow air to continue to permeate through the gaps between the particles when not under pressure; under pressure, the gaps between the particles are closed, thereby sealing the air vents of the hollow ring pipe. Compared to existing flow meters, the flow meter and its method of use of the present invention can achieve air permeability without leakage and can achieve three states: a fully permeable state, a semi-permeable state, and a fully permeable state. And a sealed state; a pressure plate structure is provided between the cover 2 and the tube 1. The pressure plate structure includes an upper pressure plate 6 connected to the cover 2 and a lower pressure plate 7 connected to the end face of the tube 1. Each of the air permeability regulating particles 5 constitutes an adjustable air permeable layer. The air permeability regulating particles 5 are arranged such that, when not under pressure, they can continue to allow air to pass through; when under pressure, they can close the air holes of the hollow ring tube 4. The present invention provides an adjusting ring tube 3, which includes a hollow ring tube 4 with a plurality of air holes and air permeability regulating particles 5 disposed within the hollow ring tube 4. The air permeability regulating particles 5 are configured such that, when not under pressure, they can continue to allow air to pass through the gaps between the particles; when under pressure, they close the gaps between the particles, allowing air to pass through. The vent holes of the closed hollow annular tube 4 ensure airtightness. The upper and lower pressure plates 6 and 7 are also equipped with a ventilating structure, which includes a ventilating channel 8 on the upper pressure plate 6, an embedded groove 9 on the lower side of the ventilating channel 8, and a sealing ball 10 on the upper side of the ventilating channel 8 that is adapted to the embedded groove 9. The upper pressure plate 6 is provided with an elastic structure for the sealing ball 10 to fit against the embedded groove 9 when under force, and to open the ventilating channel 8 when not under force. The elastic structure includes a movable pressure plate 11 disposed within and slidably connected to the upper pressure plate 6, an elastic support member 12 disposed between the movable pressure plate 11 and the upper pressure plate 6, and a force-bearing body 13 disposed on the movable pressure plate 11. The force-bearing body 13, when subjected to… When the cover 2 is tightened and pressed down, it presses down on the sealing ball 10 to seal the embedded groove 9. When the pressing force is lost, it is subjected to the reverse force of the elastic support member 12, thus restoring its original position. The structure of the lower pressure plate 7 is the same as that of the upper pressure plate 6, and the upper and lower pressure plates 6 and 7 are mirror images of each other. By setting the upper and lower pressure plates 6 and 7, they can be used in conjunction with the adjusting ring pipe 3 to achieve the three states of the present invention. While achieving air permeability, the possibility of leakage that may occur in the prior art will not occur. It should be noted that because the upper and lower pressure plates 6 and 7 have elastic support members 12, in the completely sealed state, the cover 2 needs to be tightened again to overcome the elastic support members 12 and achieve a completely sealed effect. The present invention has a simple structure, is easy to manufacture, and is easy to operate, making it suitable for widespread implementation and application.

[0026] A method for using a flow cytometer includes the following steps:

[0027] S1. Fully breathable stage: Twist the cover 2 and the tube 1 so that the lower pressure plate 7 on the tube 1 and the adjusting ring tube 3 inside the cover 2 are just in contact, forming a fully breathable state between the cover 2 and the tube 1.

[0028] S2, Semi-permeable stage: Twist the cover 2 and the tube 1 again so that the lower pressure plate 7 on the tube 1 is squeezed against the adjusting ring tube 3 structure inside the cover 2. Press the cover 2 to ensure that the cover 2 is still in a state that can move axially, forming a semi-permeable state between the cover 2 and the tube 1.

[0029] S3, Sealing state: Twist the cover 2 and the tube 1 again to press the upper and lower pressure plates 6 and 7 again, press the cover 2 to ensure that the cover 2 is in a state where it cannot move axially, thus forming a sealing state between the cover 2 and the tube 1.

[0030] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any ordinary changes and substitutions made by those skilled in the art within the scope of the technical solution of the present invention should be included within the protection scope of the present invention.

Claims

1. A flow pipe, comprising a pipe body (1) and a cap (2) threadedly connected to the pipe body (1), characterized in that, An adjustment ring tube (3) for adjusting air permeability is provided between the cover (2) and the tube (1). The adjustment ring tube (3) includes a hollow ring tube (4) with a plurality of air holes and air permeability adjustment particles (5) disposed in the hollow ring tube (4). The air permeability adjustment particles (5) are configured to continue to permeate air through the gaps between the particles when not under pressure; when under pressure, the gaps between the particles are closed, thereby closing the air holes of the hollow ring tube (4). A pressure plate structure is provided between the cover (2) and the tube (1). The pressure plate structure includes an upper pressure plate (6) connected to the cover (2) and a lower pressure plate (7) connected to the end face of the tube (1). Each of the air permeability adjustment particles (5) constitutes an adjustable air permeable layer. The air permeability adjustment particles (5) are arranged such that when not under pressure, they can continue to permeate air; when under pressure, they can close the air holes of the hollow ring tube (4). The upper pressure plate (6) and the lower pressure plate (7) are also provided with a breathable structure. The breathable structure includes a breathable channel (8) provided on the upper pressure plate (6), an inner groove (9) provided on the lower side of the breathable channel (8), and a sealing ball (10) provided on the upper side of the breathable channel (8) and adapted to the inner groove (9). The upper pressure plate (6) is provided with an elastic structure for the sealing ball (10) to fit with the inner groove (9) after being subjected to force, and to open the breathable channel (8) when not subjected to force. The elastic structure includes a movable pressure plate (11) disposed inside the upper pressure plate (6) and slidably connected to the upper pressure plate (6), an elastic support member (12) disposed between the movable pressure plate (11) and the upper pressure plate (6), and a force-bearing body (13) disposed on the movable pressure plate (11). When the force-bearing body (13) is tightened and pressed down by the cover (2), it presses down on the sealing ball (10) to seal the inner groove (9), and when the pressing force is lost, it is subjected to the reverse force of the elastic support member (12) to form a reset.

2. A flow meter according to claim 1, characterized in that, The structure of the lower pressure plate (7) is the same as that of the upper pressure plate (6), and the upper pressure plate (6) and the lower pressure plate (7) are mirror images of each other.

3. A method of using the flow meter tube according to any one of claims 1-2, characterized in that, Includes the following steps: S1, Fully breathable stage: Twist the cover (2) and the tube (1) so that the lower pressure plate (7) on the tube (1) and the adjusting ring tube (3) inside the cover (2) are just in contact, forming a fully breathable state between the cover (2) and the tube (1); S2, Semi-permeable stage: Twist the cover (2) and the tube (1) again so that the lower pressure plate (7) on the tube (1) and the adjusting ring tube (3) structure inside the cover (2) are squeezed. Press the cover (2) to ensure that the cover (2) is still in a state that can move axially, forming a semi-permeable state between the cover (2) and the tube (1); S3, Sealing state: Twist the cover and the tube body again to press the upper pressure plate (6) and the lower pressure plate (7) again, press the cover (2) to ensure that the cover (2) is in a state where it cannot move axially, thus forming a sealing state between the cover (2) and the tube body (1).

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