Composite probiotic agent for degrading cat hair, degradation method, and preparation method of freeze-dried cat snacks

Abstract

The present invention provides a composite probiotic agent for degrading cat hair, a degradation method, and a preparation method for freeze-dried cat snacks. The composite probiotic agent includes Bacillus subtilis strain CHD1 and Bacillus subtilis strain CHD2. The present invention provides a composite probiotic agent for effectively degrading cat hair. The composite probiotic agent of the present invention can effectively degrade cat hair, reduce the formation of hairballs in the cat's intestines, and reduce the risk of hairball disease. The cat hair degradation product not only provides nutrients for the strains of the probiotic agent, but also provides nutrients for the cat. In addition, the composite probiotic agent can improve the intestinal environment, promote intestinal peristalsis, and help the cat's stomach and intestines to excrete excess hair.

Description

Technical Field

[0001] The present invention relates to the field of animal food, in particular to a composite probiotic agent for degrading cat hair, a degradation method and a preparation method of freeze-dried cat snacks. Background Art

[0002] As living standards improve, more and more people keep animals. Cats were domesticated over 3,000 years ago and have become widely kept pets worldwide. Cats are extremely clean and tidy animals, and they lick their fur for self-cleaning, making licking a natural instinct. However, cats' digestive systems are not designed to digest hair. Hair enters the stomach and is not excreted, gradually forming hairballs in the intestines. Over time, this can develop into a disease known as "hairball syndrome." Common symptoms of mild hairball syndrome include: 1) frequent retching or the regurgitation of cylindrical, mucus-covered hairballs, sometimes with nothing at all; 2) dry, hard stools with bits of hair in them. Severe symptoms include poor spirits, lack of appetite, coarse, dull fur, and constipation. More severe symptoms include hairballs blocking the gastrointestinal tract, causing obstruction, dehydration, and organ failure.

[0003] Pets are becoming increasingly important in families, and their health is receiving increasing attention. To prevent or treat hairballs in cats, owners need to regularly feed their cats hairball removers or cat grass to help them expel hairballs. However, the hairball removers currently sold on the market not only have poor palatability, but also mostly contain chemical additives and preservatives, and their main ingredient is mineral oil. While mineral oil has a lubricating effect, helping cats vomit out hairballs smoothly, cats cannot absorb or digest it. If hairball removers are fed too frequently or in excessive amounts, a difficult-to-break-down film will form on the cat's intestines, affecting the intestinal absorption of nutrients and damaging the cat's own stomach balance, causing gastrointestinal disorders. Summary of the Invention

[0004] The present invention aims to provide a composite probiotic agent that effectively degrades cat hair, a degradation method, and a method for preparing freeze-dried cat snacks. The composite probiotic agent degrades cat hair in the cat's intestines, reducing the risk of hairball formation, improving the intestinal environment, promoting intestinal motility, and helping to excrete excess hair and other waste from the cat's stomach and intestines.

[0005] The composite probiotic agent for degrading cat hair of the present invention comprises Bacillus subtilis strain CHD1 and Bacillus subtilis strain CHD2.

[0006] Preferably, the Bacillus subtilis CHD1 and the Bacillus subtilis CHD2 are mixed in a quantitative ratio of 1:1.

[0007] Preferably, the preparation method of the composite probiotic agent is: Bacillus subtilis CHD1 and Bacillus subtilis CHD2 are respectively inoculated into a probiotic culture medium for cultivation, and cultured to the logarithmic growth phase to obtain a probiotic culture solution; the probiotic culture solution is centrifuged to obtain bacterial cells, and then Bacillus subtilis CHD1 and Bacillus subtilis CHD2 are mixed in a quantity ratio of 1:1 to obtain the composite probiotic agent.

[0008] Preferably, the probiotic culture medium is prepared by the following raw materials in percentage by weight: 10% tryptone, 5% yeast extract, 10% NaCl, and the balance water.

[0009] Preferably, the inoculated probiotic culture medium is cultured at 37° C. and 180 rpm.

[0010] Preferably, the centrifugation is carried out at 6000 rpm for 10 min.

[0011] A second object of the present invention is to provide a method for degrading cat hair, which uses the above-mentioned composite probiotic agent to degrade cat hair.

[0012] Preferably, the above-mentioned composite probiotic agent is inoculated into a cat hair degradation culture medium to degrade cat hair. The cat hair degradation culture medium contains, by weight, 300 mg of cat hair, 3.821 mg of potassium chloride, 11.416 mg of dipotassium hydrogen phosphate trihydrate, 6.162 mg of magnesium sulfate heptahydrate, 0.279 mg of sodium iron EDTA, 0.039 mg of boric acid, 0.008 mg of manganese sulfate monohydrate, 0.003 mg of copper sulfate, 0.014 mg of zinc sulfate monohydrate, 0.003 mg of sodium molybdate, and the balance is water per 25 ml.

[0013] The third object of the present invention is to provide a method for preparing freeze-dried cat snacks, which comprises inoculating the above-mentioned composite probiotic agent into freeze-dried cat snack raw materials for fermentation to obtain freeze-dried cat snacks.

[0014] Preferably, the specific preparation method is:

[0015] S1 measured according to the following weight percentage of chicken paste raw materials: chicken breast 69.9%, 10% oats, 10% barley, 5% oat grass, 5% barley grass, 0.1% catnip, raw materials mixed minced cooked set aside;

[0016] S2 measured according to the following weight percentage of chicken liver paste raw materials: chicken liver 69.9%, 10% oats, 10% barley, 5% oat grass, 5% barley grass, 0.1% catnip, raw materials mixed minced cooked set aside;

[0017] S3 measured according to the following percentage by weight of salmon paste raw materials: salmon 69.9%, 10% oats, 10% barley, 5% oat seedlings, 5% barley seedlings, 0.1% catnip, raw materials mixed minced cooked set aside;

[0018] S4 measured according to the following weight percentage of bonito paste raw materials: bonito 69.9%, 10% oats, 10% barley, 5% oat seedlings, 5% barley seedlings, 0.1% catnip, raw materials mixed minced and cooked set aside;

[0019] S5 were added to the chicken paste, chicken liver paste, salmon paste and bonito paste by weight percentage of the composite probiotic agent 10%, were mixed into squares and cut into small pieces, freeze-dried;

[0020] S6. Cat snacks were prepared according to the following weight percentages: 50% freeze-dried chicken chunks, 10% freeze-dried chicken liver chunks, 20% freeze-dried salmon chunks, and 20% freeze-dried bonito chunks.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. The present invention provides a composite probiotic agent that effectively degrades cat hair. This composite probiotic agent can effectively degrade cat hair, reducing the formation of hairballs in the cat's intestines and lowering the risk of hairball syndrome. The degraded cat hair provides nutrients not only for the probiotic strains but also for the cat itself. Furthermore, the composite probiotic agent can improve the intestinal environment, promote intestinal motility, and help expel excess hair from the cat's stomach and intestines.

[0023] 2. The cat snacks provided by the present invention contain a large amount of animal protein, which can enhance the cat's physique and resistance, thereby improving immunity and vitality. In addition, the cat snacks contain a small amount of catnip and a large amount of dietary fiber, which can further promote intestinal peristalsis, help cats excrete waste from the stomach and intestines, and prevent constipation.

[0024] 3. The cat snacks provided by the present invention are made from abundant and natural raw materials, are non-toxic and non-irritating to cats. The cat snacks in the present invention have simple raw materials and are easy to prepare and operate, making them easy to produce industrially.

[0025] Bacillus subtilis strain CHD1 and Bacillus subtilis strain CHD2 are disclosed in NCBI with accession numbers OP787953.1 and OP787954.1, respectively. The applicant also holds the copies of these two strains and guarantees that they will be released to the public within 20 years from the date of application. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1This is a diagram showing the degradation effect of the probiotic agents obtained in Examples 1 to 3 on cat hair;

[0027] Figure 2 is the amino acid content in the degraded cat hair culture solution obtained in Examples 1 to 3;

[0028] Figure 3 is the degradation amount of cat hair by the probiotic agents obtained in Examples 1 to 3;

[0029] Among them, Example 1 is a treatment with a composite probiotic agent, Example 2 is a treatment with Bacillus subtilis CHD1, and Example 3 is a treatment with Bacillus subtilis CHD2. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] Example 1

[0032] The preparation of a composite probiotic agent that effectively degrades cat hair comprises the following steps:

[0033] S1. Prepare a probiotic culture medium using the following weight percentages: 10% tryptone, 5% yeast extract, 10% NaCl, and the balance water. Mix the ingredients thoroughly, adjust the pH to 7.0 with NaOH solution, and sterilize under autoclave at 121°C for 15 minutes before use.

[0034] S2. Bacillus subtilis CHD1 and Bacillus subtilis CHD2 were inoculated into the probiotic culture medium and cultured separately at 37°C and 180 rpm until the logarithmic growth phase. 600 Measure the OD of the bacterial solution and adjust the OD values ​​of the two with sterile water to make their OD equal to obtain the probiotic culture solution.

[0035] S3. Centrifuge the culture broth of two probiotic strains with equal OD at 6000 rpm for 10 min respectively, discard the supernatant, and obtain the probiotic cells.

[0036] S4. Bacillus subtilis CHD1 and Bacillus subtilis CHD2 are mixed in a quantity ratio of 1:1 to obtain a composite probiotic agent.

[0037] S5. Degraded cat hair culture medium was prepared according to the following weight of raw materials (mg / 25mL): 300g cat hair, 3.82l potassium chloride, 11.416g potassium hydrogen phosphate trihydrate, 6.162g magnesium sulfate heptahydrate, 0.27g sodium iron EDTA, 0.03g boric acid, 0.008g manganese sulfate monohydrate, 0.003g copper sulfate, 0.014g zinc sulfate monohydrate, 0.003g sodium molybdate, and the balance water. After mixing the ingredients evenly, sterilize and set aside.

[0038] S6. Add 10% by mass of the composite probiotic agent to the cat hair degradation culture medium and culture at 37°C and 180 rpm for 7 days to obtain a cat hair degradation culture medium. A culture medium without the composite probiotic agent was used as a control.

[0039] S7. Centrifuge the degraded cat hair culture medium at 6000 rpm for 10 min. The supernatant is used to detect the amino acid content, and the precipitate is dried and used to detect the amount of cat hair degradation.

[0040] A method for preparing a compound probiotic freeze-dried cat snack that effectively degrades cat hair comprises the following steps:

[0041] S1 measured according to the following weight percentage of chicken paste raw materials: chicken breast 69.9%, 10% oats, 10% barley, 5% oat grass, 5% barley grass, 0.1% catnip, raw materials mixed minced cooked set aside;

[0042] S2 measured according to the following weight percentage of chicken liver paste raw materials: chicken liver 69.9%, 10% oats, 10% barley, 5% oat grass, 5% barley grass, 0.1% catnip, raw materials mixed minced cooked set aside;

[0043] S3 measured according to the following percentage by weight of salmon paste raw materials: salmon 69.9%, 10% oats, 10% barley, 5% oat seedlings, 5% barley seedlings, 0.1% catnip, raw materials mixed minced cooked set aside;

[0044] S4 measured according to the following weight percentage of bonito paste raw materials: bonito 69.9%, 10% oats, 10% barley, 5% oat seedlings, 5% barley seedlings, 0.1% catnip, raw materials mixed minced and cooked set aside;

[0045] S5 were added to the chicken paste, chicken liver paste, salmon paste and bonito paste by weight percentage of the composite probiotic agent 10%, were mixed into squares and cut into small pieces, freeze-dried to obtain freeze-dried chicken pieces, freeze-dried chicken liver pieces, freeze-dried salmon pieces and freeze-dried bonito pieces;

[0046] S6. Cat snacks were prepared according to the following weight percentages: 50% freeze-dried chicken chunks, 10% freeze-dried chicken liver chunks, 20% freeze-dried salmon chunks, and 20% freeze-dried bonito chunks, and were vacuum-packed and stored.

[0047] Example 2

[0048] This embodiment is basically the same as Example 1, except that Bacillus subtilis CHD1 is used instead of the composite probiotic agent.

[0049] Example 3

[0050] This embodiment is basically the same as Example 1, except that Bacillus subtilis CHD2 is used instead of the composite probiotic agent.

[0051] pass Figure 1 The results showed that compared with the control group (not containing probiotics), the probiotics obtained in Examples 1 to 3 all showed a degradation effect on cat hair, among which the composite probiotics obtained in Example 1 had the most obvious degradation effect on cat hair.

[0052] After 7 days of culture, the degraded cat hair culture medium of Examples 1 to 3 was centrifuged at 6000 rpm for 10 min, and the supernatant was taken and diluted 10 times. The amino acid content was detected at 570 nm according to the instructions of the amino acid content detection kit (Beijing Solebow Technology Co., Ltd., Beijing). Figure 2 As can be seen, the amino acid content in the supernatant of the cat hair degraded culture solution of Examples 1 to 3 was 101.67, 106.94, and 122.22 μmol / mL, respectively. This indicates that the probiotic agents obtained in Examples 1 to 3 degrade cat hair into short peptides and amino acids in a culture medium containing no other carbon and nitrogen sources besides cat hair, and use these degradation products as carbon and nitrogen sources to sustain their own growth.

[0053] The degraded cat hair culture solution of Examples 1 to 3 after 7 days of culture was centrifuged at 6000 rpm for 10 minutes, and the precipitate was dried at 80°C. The dried cat hair was weighed to detect the amount of cat hair degradation. Figure 3 It can be seen that after 7 days of culture, the probiotic agents obtained in Examples 1 to 3 all effectively degraded cat hair, among which the composite probiotic agent obtained in Example 1 had the highest degradation rate of cat hair, which was 109.5 mg. These results show that the composite probiotic agent of the present invention is a probiotic agent that can effectively degrade cat hair.

Claims

1. A composite probiotic agent for degrading cat hair, characterized in that: By Bacillus subtilis ( Bacillus subtilis ) strain CHD1 and Bacillus subtilis ( Bacillus subtilis ) strain CHD2 is mixed in a quantity ratio of 1:

1.

2. The composite probiotic agent according to claim 1, characterized in that The preparation method of the composite probiotic agent comprises the following steps: respectively inoculating Bacillus subtilis CHD1 and Bacillus subtilis CHD2 into a probiotic culture medium for culturing until the logarithmic growth phase to obtain a probiotic culture solution; centrifuging the probiotic culture solution to obtain bacterial cells, and then mixing Bacillus subtilis CHD1 and Bacillus subtilis CHD2 in a quantity ratio of 1:1 to obtain the composite probiotic agent.

3. The composite probiotic agent according to claim 2, characterized in that The probiotic culture medium is prepared by raw materials in the following weight percentages: 10% tryptone, 5% yeast extract, 10% NaCl, and the balance is water.

4. The composite probiotic agent according to claim 2 or 3, characterized in that The inoculated probiotic culture medium is cultured at 37° C. and 180 rpm.

5. The composite probiotic agent according to claim 2, characterized in that The centrifugation condition is 6000 rpm for 10 min.

6. A method for degrading cat hair, characterized in that: The composite probiotic agent according to claim 1 is used to degrade cat hair.

7. The method according to claim 6, characterized in that The method is to inoculate a composite probiotic agent into a cat hair degradation culture medium to degrade cat hair. The cat hair degradation culture medium contains, by weight, 300 mg of cat hair, 3.821 mg of potassium chloride, 11.416 mg of dipotassium hydrogen phosphate trihydrate, 6.162 mg of magnesium sulfate heptahydrate, 0.279 mg of sodium iron EDTA, 0.039 mg of boric acid, 0.008 mg of manganese sulfate monohydrate, 0.003 mg of copper sulfate, 0.014 mg of zinc sulfate monohydrate, 0.003 mg of sodium molybdate, and the balance is water per 25 ml.

8. A method for preparing freeze-dried cat snacks, characterized in that: The composite probiotic agent according to claim 1 is inoculated into freeze-dried cat snack raw materials for fermentation to prepare freeze-dried cat snacks.

9. The preparation method according to claim 8, characterized in that The specific preparation method is: S1 measured according to the following weight percentage of chicken paste raw materials: chicken breast 69.9%, 10% oats, 10% barley, 5% oat grass, 5% barley grass, 0.1% catnip, raw materials minced and cooked together; S2 measured according to the following weight percentage of chicken liver paste raw materials: chicken liver 69.9%, 10% oats, 10% barley, 5% oat grass, 5% barley grass, 0.1% catnip, raw materials mixed minced cooked standby; S3 measured according to the following percentage by weight of salmon paste raw materials: salmon 69.9%, 10% oats, 10% barley, 5% oat seedlings, 5% barley seedlings, 0.1% catnip, raw materials minced and cooked together; S4 measured according to the following weight percentages of bonito paste raw materials: bonito 69.9%, 10% oats, 10% barley, 5% oat seedlings, 5% barley seedlings, 0.1% catnip, raw materials mixed minced and cooked set aside; S5 were added to the chicken paste, chicken liver paste, salmon paste and bonito paste by weight percentage of the composite probiotic agent 10%, were mixed into squares and cut into small pieces, freeze-dried; S6. Cat treats were prepared according to the following weight percentages: 50% freeze-dried chicken chunks, 10% freeze-dried chicken liver chunks, 20% freeze-dried salmon chunks, and 20% freeze-dried bonito chunks.

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