Herbicidal composition and method for controlling herbicide-resistant weeds

Abstract

The present invention relates to a method for effectively controlling unwanted weeds. In particular, the present invention relates to a method for controlling unwanted weeds that are resistant to herbicides.

Description

Technical Field

[0001] The present invention relates to the field of plant protection, in particular to a method for effectively controlling undesirable weeds. More particularly, the present invention relates to a method for selectively controlling undesirable vegetation that is resistant to herbicides. Background Art

[0002] Weeds are unwelcome plants that can seriously damage crop yields. Farmers usually control these plants before planting and after sowing. Modern herbicides are used to control or suppress these unwelcome plants so that more nutrients are available to the sown crops.

[0003] Protecting planted crops from unwelcome plants that inhibit crop growth is a long-standing struggle for all farmers. Herbicides have been used to control the growth of such plants. Many herbicides and combinations are known in the art.

[0004] Current practice involves combining herbicides with different modes of action to expand the scope of control and resistance management. However, currently known combinations are insufficient for controlling resistant and persistent weeds. There is a continuing need in the art for improved herbicidal compositions with enhanced weed control efficacy.

[0005] However, herbicide combinations do not always achieve the desired effect. Herbicide combinations may result in additive or antagonistic effects. It may also result in phytotoxicity to crops, making it an undesirable combination. Therefore, agronomists must carefully select herbicides that can be combined to provide a synergistic effect in controlling weeds while having no phytotoxic effects on crops and reducing the chance of developing herbicide-resistant weeds. Herbicide tank mixes can be used to prevent the development of herbicide-resistant weeds by attacking weeds at more than one lethal site of action.

[0006] However, tank mixing may also lead to unintended interactions between herbicides, such as antagonism.

[0007] Glufosinate is a glutamine synthase inhibitor herbicide or Group H inhibitor herbicide, which is a broad-spectrum post-emergence herbicide that controls most annual grasses and broadleaf plants. It is a contact herbicide with limited migration throughout the plant. Glutamine synthase herbicides inhibit the activity of glutamine synthase, the enzyme that converts glutamate and ammonia to glutamine. Glufosinate is a good resistance management tool. It is primarily used for post-emergence control of grass and grass weeds.

[0008] Glycine (glyphosate) is a herbicide that inhibits 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, a key enzyme in the shikimate pathway that participates in the synthesis of aromatic amino acids. Glyphosate [N-(phosphonomethyl)glycine] is a non-selective, broad-spectrum, systemic, post-emergence herbicide that has been widely used for weed management throughout the world over the past three decades. It inhibits the biosynthesis of aromatic amino acids (phenylalanine, tryptophan, and tyrosine), leading to several metabolic disorders, including inhibition of protein and secondary product biosynthesis and dysregulation of the shikimate pathway, leading to general metabolic disorders.

[0009] Intensive glyphosate selection has led to the widespread evolution of glyphosate-resistant (GR) weed populations, threatening the sustainability of this valuable once-in-a-century agrochemical. The management of GR weeds is a challenge, and the widespread occurrence of GR weeds in multiple countries requires alternative weed management programs.

[0010] ACCase inhibitors are mainly used for post-emergence weed control in broadleaf crops. These herbicides inhibit acetyl-CoA carboxylase (ACCase), which catalyzes the first step in fatty acid synthesis and is important for membrane synthesis. At least 43 grass weeds are known to be resistant to acetyl-CoA carboxylase herbicides. When weeds become resistant to ACCase inhibitors, their management becomes more challenging due to the lack of alternative MoA (mechanism of action) that can be used for post-emergence selective weed control. In addition, when the resistance mechanism involves herbicide metabolism, they can confer resistance to multiple herbicide modes of action.

[0011] Various weed species have developed resistance to many herbicides. Goosegrass, an annual grass, is considered one of the top ten "most important herbicide-resistant species" and its population has developed resistance to multiple herbicides, including PSI electron diverter (D / 22), ACCase inhibitor (A / 1), EPSP synthase inhibitor (G / 9), glutamine synthase inhibitor (H / 10), microtubule inhibitor (K1 / 3), PPO inhibitor (E / 14), multiple resistance: 2 sites of action - PSI electron diverter (D / 22), glutamine synthase inhibitor (H / 10), etc. (http: / / weedscience.org).

[0012] Goosegrass (Eleusine indica) is one of the most common and troublesome weeds worldwide. Goosegrass is a monocotyledonous weed in the Poaceae family. Glyphosate-resistant (GR) goosegrass has recently been confirmed in Brazil. These specific biotypes are known to be resistant to fenoxaprop-ethyl, glyphosate, and haloxyfop-ethyl, and they may have cross-resistance to other herbicides in the A / 1 and G / 9 families. This GR weed species needs to be controlled by more than one herbicide in a program, which can be difficult and challenging because the range of effective herbicide options is very limited.

[0013] Sea crabgrass, commonly known as two-eared grass, is one of the most troublesome weeds in South America due to glyphosate resistance and is widely distributed in most crop production areas.

[0014] Herbicides have become increasingly important in agriculture, and this reliance has led to an increase in herbicide-resistant weed populations.

[0015] Interactions between glyphosate and glufosinate have been previously reported (Chuah et al., 2008; Kudsk and Mathiassen, 2004). Antagonism was observed between these two herbicides in goosegrass at all rates studied (Chuah et al., 2008). This antagonism has been attributed to the rapid action of glufosinate, which causes plant injury before the slower systemic glyphosate can take effect. A holistic strategy for managing herbicide-resistant weeds is essential for agricultural sustainability and increasing crop yields, which will provide food, fiber, and energy for a growing population. Integrated weed management is a combination of multiple weed control methods in order to reduce weed nuisance to below economic threshold levels. Therefore, when weeds become resistant to herbicides, their management becomes more challenging due to the lack of alternative modes of action that can be used for post-emergence selective weed control.

[0016] Therefore, there remains a pressing need for improved methods to prevent, delay or effectively manage the growth of herbicide-resistant weeds, thereby increasing overall yield and plant health while reducing phytotoxicity. Therefore, there is also a need to develop herbicide combination strategies that are synergistic and helpful for herbicide resistance management.

[0017] Therefore, embodiments of the present invention can improve one or more of the above problems:

[0018] Purpose of the Invention

[0019] An object of the present invention is to provide a method for effectively controlling herbicide-resistant weeds.

[0020] Another object of the present invention is to provide a method of controlling herbicide resistant weeds, wherein the method comprises applying a combination of herbicides simultaneously or subsequently or sequentially to target the weeds.

[0021] It is a further object of the present invention to improve methods of controlling the growth of herbicide resistant weeds and to address associated problems such as poor herbicide control and phytotoxicity.

[0022] Another object of the present invention is to provide a method for controlling herbicide-resistant weeds, wherein the method does not adversely affect the normal growth of useful plants, in particular crop plants, growing in the vicinity of or in the environment of the weeds.

[0023] An advantageous object of the present invention is to provide an effective preventive and therapeutic method for controlling herbicide-resistant weeds that is superior to conventional methods.

[0024] The above and related objects of the invention will become apparent from the description of the invention set forth hereinafter. Summary of the invention

[0025] In one aspect, the present invention relates to the field of plant protection, in particular to a method for effectively controlling undesirable weeds.

[0026] In another aspect, the present invention provides herbicidal compositions which exhibit enhanced herbicidal action against undesirable harmful plants and / or have improved compatibility with plants, in particular crops.

[0027] Another aspect of the present invention is to provide a method for effectively controlling herbicide-resistant weeds, particularly weeds resistant to 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase-inhibiting herbicides, glutamine synthase-inhibiting herbicides and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides in agricultural fields.

[0028] In another aspect, the present invention provides a method of controlling the growth of herbicide-resistant weeds comprising contacting such weeds or parts thereof or their immediate environment with an essential herbicide combination as provided below.

[0029] Thus, in one aspect, the present invention provides a method of controlling herbicide-resistant weeds by applying a combination of herbicides to a locus simultaneously, or subsequently, or sequentially, the method comprising;

[0030] (i) comprising a first application of one or more herbicides selected from 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibiting herbicides and / or glutamine synthase inhibiting herbicides,

[0031] (ii) a second application comprising one or more herbicides selected from the group consisting of a 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibiting herbicide, a glutamine synthase inhibiting herbicide, and an acetyl-CoA carboxylase (ACCase) inhibiting herbicide;

[0032] wherein the second administration is administered at least 7 days after the first administration.

[0033] In another aspect, the present invention provides a method of controlling weeds resistant to herbicides, wherein the weeds are treated by simultaneously or subsequently or sequentially applying a combination comprising a herbicide to target the weeds.

[0034] In another aspect, the present invention provides a method for inhibiting, controlling, preventing, delaying and reducing the population of grass weeds, particularly goosegrass and crabgrass, by simultaneously or sequentially applying a combination of herbicides to target the weeds.

[0035] In another aspect, the present invention provides an effective and rapid method for controlling herbicide resistant weeds, particularly goosegrass and crabgrass.

[0036] In another aspect, the present invention provides a method of controlling goosegrass weeds, wherein the method comprises applying a combination of herbicides simultaneously or subsequently or sequentially to target the weeds.

[0037] In another aspect, the present invention improves the control method and solves problems such as poor control effect, effective prevention and treatment purposes, which is superior to conventional methods.

[0038] In another aspect, the invention provides a method that does not adversely affect the normal growth of the plant.

[0039] In one aspect, the invention provides a method for controlling herbicide-resistant weeds, which can be applied pre-emergence or post-emergence.

[0040] Still another aspect of the present invention is to provide a method for increasing the yield of crops by applying the method using the herbicidal combination.

[0041] Another aspect of the present invention is to provide a method for improving crop health by applying a herbicidal combination.

[0042] In another aspect, the present invention provides a complete strategy for managing herbicide-resistant weeds to achieve agricultural sustainability and increase crop yields. DETAILED DESCRIPTION

[0043] For the purpose of the following detailed description, it should be understood that the present invention can take various alternative variations, unless there is a clear provision to the contrary. In addition, except in any operating examples, or where otherwise indicated, all numbers representing the quantity of materials / ingredients used in the specification should be understood to be modified by the term "about" in all cases. The term "about" used to define the amount of the active component should be interpreted as meaning "approximately" or "reasonably close" and any statistically insignificant changes.

[0044] As used herein, the terms "including," "comprising," "having," "containing," "involving," etc. should be construed as open-ended, meaning including but not limited to.

[0045] In any aspect or embodiment described below, the phrase comprising may be replaced by the phrase "consisting of" or "consisting essentially of. In these aspects or embodiments, the combination or composition comprises or includes the specific components listed therein, or consists of or consists essentially of or consists essentially of, to the exclusion of other ingredients or excipients not specifically listed therein.

[0046] As used herein, the term herbicide shall mean an active ingredient that kills, controls or otherwise adversely modifies the growth of undesirable vegetation.

[0047] As used herein, an herbicidally effective amount or vegetation controlling amount is an amount of the active ingredient that causes a "herbicidal effect," ie, an adversely altered effect, and includes departures from natural development, killing, regulation, desiccation, delayed development, stunting, and the like.

[0048] The terms "plant" and "vegetation" include, but are not limited to, germinating seeds, emerging seedlings, plants emerging from vegetative propagules, and established vegetation.

[0049] As used herein, the terms "unwelcome plants," "noxious plants," "unwanted plants," "weeds," and "weed species" are synonymous.

[0050] As used herein, the term "locus" refers to the vicinity of a desired crop where weed control, typically selective weed control, is desired. The locus includes the vicinity of desired crop plants where weed infestation has occurred or is about to occur.

[0051] The term "crop" shall include a plurality of desired crop plants or an individual crop plant growing at a particular location.

[0052] Each of the aspects described above may have one or more embodiments.

[0053] Each embodiment described below can be applied to one or all aspects described above. These embodiments are intended to be understood as preferred features of one or all aspects described above. Each embodiment described below is respectively applicable to each aspect described above.

[0054] Glyphosate-resistant (GR) goosegrass has recently been identified as a weed in Brazil. It has been observed that farmers have no options for controlling this weed because it is resistant to systemic herbicides such as glyphosate or ACCase herbicides and other contact herbicides. Two-ear grass is also one of the most difficult weeds because it is glyphosate-resistant. According to the present invention, an effective and rapid method for controlling this weed is provided. The method comprises sequential application of a herbicide combination, which effectively controls this weed.

[0055] Surprisingly, the present inventors have discovered that the combination of a Group G inhibitor (5-enolpyruvylshikimate-3-phosphate (EPSP)), i.e., glyphosate, with another herbicide selected from the Group A inhibitors (acetyl-CoA carboxylase (ACCase) inhibiting herbicides) and the Group H (glutamine synthase inhibiting herbicides) inhibitors, when applied in a sequential manner, results in effective control of herbicide-resistant weeds at the locus of desired crop plants. When these herbicides are applied in a sequential manner as described in the present invention, the phytotoxicity normally seen when applying glyphosate and glufosinate or a combination thereof to control weeds is also eliminated.

[0056] In one embodiment, the present invention provides preferred combinations, compositions and methods thereof. The methods of the present invention include methods of controlling weeds at a location by applying the combination or the composition to the location, or methods of increasing the yield of a crop by applying the combination or the composition, or methods of improving plant health by applying the combination or the composition at the location of the plant. The embodiments described herein describe preferred embodiments of all these possible combinations, compositions and methods of the present invention.

[0057] Thus, in one aspect, the present invention provides a method of controlling herbicide-resistant weeds by applying a combination of herbicides to a locus simultaneously, sequentially or sequentially, the method comprising,

[0058] (i) comprising a first application of one or more herbicides selected from 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibiting herbicides and / or glutamine synthase inhibiting herbicides, and

[0059] (ii) a second application comprising one or more herbicides selected from the group consisting of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibiting herbicides, glutamine synthase inhibiting herbicides and acetyl-CoA carboxylase (ACCase) inhibiting herbicides.

[0060] Therefore, in one aspect, the present invention provides a herbicidal composition comprising one or more herbicides selected from 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibiting herbicides and / or glutamine synthase inhibiting herbicides, and one or more herbicides selected from 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibiting herbicides, glutamine synthase inhibiting herbicides and acetyl-CoA carboxylase (ACCase) inhibiting herbicides. The present invention also relates to methods and uses for controlling unwelcome vegetation, particularly unwelcome vegetation in crops.

[0061] In another aspect, the present invention provides a method for effectively controlling herbicide-resistant weeds, wherein the herbicide-resistant weeds are resistant to at least one herbicide belonging to Group A (ACCase inhibiting herbicides), Group G (5-enolpyruvylshikimate-3-phosphate (EPSP) inhibiting herbicides) and Group H (glutamine synthase inhibiting herbicides) inhibitors.

[0062] Typically, the target weeds of the present invention are resistant to glyphosate, ACCase herbicides, glufosinate-ammonium, and the like.

[0063] The present invention provides a method for controlling herbicide-resistant weeds, particularly grass weeds.

[0064] In a preferred embodiment, the herbicide resistant weed is Goosegrass.

[0065] In a preferred embodiment, the herbicide resistant weed is Digitaria tangutica (Digitaria tangutica).

[0066] In another aspect, the present invention provides an efficient and rapid method for controlling goosegrass and crabgrass that are resistant to Group A, G, and H herbicides.

[0067] In one embodiment, the Group A or ACCase inhibiting herbicide is selected from aryloxyphenoxypropionate (FOP), cyclohexanedione (DIM), and phenylpyrazole (DEN).

[0068] In one embodiment, the aryloxyphenoxypropionate is selected from clodinafop-butyl, cyhalofop-butyl, fluazifop-butyl, oxadiazol-butyl, fluazifop-butyl and quizalofop-butyl.

[0069] In one embodiment, the cyclohexanedione is selected from clethodim and sethoxydim.

[0070] In one embodiment, the phenylpyrazoline is pinoxaden.

[0071] In one embodiment, the Group G or 5-enolpyruvylshikimate-3-phosphate EPSP synthase inhibitor is selected from the organophosphorus chemical family.

[0072] In one embodiment, the EPSP synthase inhibitor is glyphosate.

[0073] In one embodiment, the Group H or glutamine synthase inhibitor is selected from the organophosphorus chemical family.

[0074] In one embodiment, the glutamine synthase inhibitor is glufosinate.

[0075] Preferably, the composition according to the invention comprises glufosinate in free form or in salt form, in particular the potassium, sodium, ammonium, trimethylsulfonium, monoethanolammonium, isopropylammonium, triazolamide salts. Preferably, glufosinate is used as a selective herbicide.

[0076] In another aspect, the present invention provides a method of controlling weeds resistant to herbicides, wherein the weeds are treated by simultaneously or sequentially applying herbicidally effective amounts of at least two herbicides to target the weeds.

[0077] In another aspect, the present invention provides a method of controlling weeds resistant to herbicides, wherein the weeds are treated by simultaneously or sequentially applying a composition comprising a herbicidally effective amount of a combination of herbicides to the locus of crop plants.

[0078] Preferably, the method comprises applying glyphosate and sequentially applying glufosinate to effectively control the herbicide resistant weed, i.e., goosegrass.

[0079] In one embodiment, the methods of the present invention provide significant control of herbicide resistant weeds within 10 to 30 days.

[0080] In one embodiment, the method comprises applying a herbicidally effective amount of the herbicide combination within 10 to 30 days.

[0081] In one embodiment, the method comprises applying a herbicidally effective amount of a combination of herbicides simultaneously or sequentially within 10 to 30 days, preferably within 10 to 15 days.

[0082] According to the method of the present invention, adult plants of herbicide-resistant goosegrass are best controlled by applying glyphosate followed by another herbicide, glufosinate, at intervals of 10 to 30 days, preferably within 10 to 15 days.

[0083] The combination can be applied to the locus of weeds in a herbicidally effective amount. The selection of a suitable effective amount depends on the density of the weed infestation, weather patterns, crop health and many other factors.

[0084] In one embodiment, glufosinate or glyphosate or a salt thereof may be used in an amount of about 1 g / ha to about 5000 g / ha.

[0085] In one embodiment, glyphosate may be used in an amount of about 5 g / ha to about 500 g / ha.

[0086] In one embodiment, glyphosate may be used in an amount of about 200 g / ha.

[0087] In one embodiment, glyphosate may be used in an amount of about 400 g / ha.

[0088] In one embodiment, glufosinate may be used in an amount of about 5 g / ha to about 500 g / ha.

[0089] In one embodiment, glufosinate may be used in an amount of about 100 g / ha.

[0090] In one embodiment, glufosinate may be used in an amount of about 200 g / ha.

[0091] In one embodiment, the herbicide of the present invention may be applied together with at least one other active ingredient selected from the group consisting of herbicides, insecticides, fungicides, biological agents, plant growth activators, fertilizers, or combinations thereof.

[0092] The composition may further include at least one agrochemically acceptable excipient.

[0093] The dosage level of an individual herbicide may be dictated by the intrinsic efficacy of the compound, pest pressure, soil type, weather, formulation type, and many other uncertain variables. These standard dosage levels are established by regulatory agencies.

[0094] These compositions can be applied by spraying onto the weeds to be treated. The application dosage will of course depend on the nature of the crop and the weeds to be treated. For example, these herbicidal compositions can be applied at a rate of 0.1 to 10 L / ha, preferably 0.5 to 8 L / ha, even more preferably 1 to 5 L / ha, typically 3 L / ha of the crop to be treated.

[0095] In one embodiment, the individual components of the combination of the present invention may be applied to the locus simultaneously or sequentially such that glyphosate and glufosinate may be applied within 10 to 30 days of each other as a tank mix or premix composition.

[0096] The herbicidal composition of the present disclosure may be in any conventional agriculturally useful form, for example, in the form of a ready-to-use formulation or in the form of a tank mix.

[0097] In one embodiment, the herbicidal composition of the present disclosure is a tank mix.

[0098] In one embodiment, the combination of the present invention can be applied before or after emergence. When applied after emergence, the advantage of the combination is a surprisingly good residual effect, resulting in rapid control of weeds.

[0099] In one embodiment, the composition of the present invention can contain agriculturally acceptable adjuvants, carriers, diluents, emulsifiers, fillers, defoamers, thickeners, antifreeze agents, refrigerants, etc. The composition can be solid or liquid. They can be solid, such as, for example, dust, particles, water-dispersible particles, microcapsules or wettable powders, or liquids, such as, for example, emulsifiable concentrates, solutions, emulsions or suspensions, ZC formulations. They can also be provided as premixes or tank mixes.

[0100] Suitable agricultural adjuvants and carriers may include, but are not limited to, crop oil concentrates; methylated seed oil, emulsified methylated seed oil, nonylphenol ethoxylate; benzyl coconut oil alkyl dimethyl quaternary ammonium salt; blends of petroleum hydrocarbons, alkyl esters, organic acids and anionic surfactants; C9-C11 alkyl polyglycosides; phosphated alcohol ethoxylates; natural primary alcohol (C12-C16) ethoxylates; di-sec-butylphenol EO-PO block copolymers; methyl-terminated polysiloxanes; nonylphenol polyoxyethylene ether, urea ammonium nitrate; tridecanol (synthetic) ethoxylate (8EO); tallow amine ethoxylate; PEG (400) dioleate-99, alkyl sulfates, such as diethanolammonium lauryl sulfate; alkyl aryl sulfonates, such as calcium dodecylbenzenesulfonate; alkylphenol-oxyalkylene addition products, such as nonylphenol-C1 8 ethoxylates; alcohol-alkylene oxide addition products, such as tridecanol-C16 ethoxylate; soaps, such as sodium stearate; alkyl-naphthalene-sulfonates, such as sodium dibutylnaphthalene sulfonate; dialkyl esters of sulfosuccinates, such as sodium di(2-ethylhexyl)sulfosuccinate; sorbitol esters, such as sorbitol oleate; quaternary amines, such as lauryltrimethylammonium chloride; polyethylene glycol esters of fatty acids, such as polyethylene glycol stearate; block copolymers of ethylene oxide and propylene oxide; salts of monoalkyl and dialkyl phosphates; vegetable oils or seed oils, such as soybean oil, rapeseed / low-erucic acid canola oil, olive oil, castor oil, sunflower oil, coconut oil, corn oil, cottonseed oil, linseed oil, palm oil, peanut oil, safflower oil, sesame oil, tung oil, etc.; and esters of the above vegetable oils, and in certain embodiments, including methyl esters.

[0101] Suitable liquid carriers that can be used in the compositions of the present invention may include water or organic solvents. Organic solvents include, but are not limited to, petroleum fractions or hydrocarbons, such as mineral oil, aromatic solvents, paraffin oil, etc.; vegetable oils, such as soybean oil, rapeseed oil, olive oil, castor oil, sunflower oil, coconut oil, corn oil, cottonseed oil, linseed oil, palm oil, peanut oil, safflower oil, sesame oil, tung oil, etc.; esters of the above vegetable oils; esters of monohydric alcohols or dihydric, trihydric or other low polyhydric alcohols (containing 4-6 hydroxyl groups), such as 2-ethylhexyl stearate, n-butyl oleate, isopropyl myristate, propylene glycol dioleate, dioctyl succinate, dibutyl adipate, dioctyl phthalate, etc.; esters of monohydric, dihydric and polyhydric carboxylic acids, etc. Organic solvents include but are not limited to toluene, xylene, naphtha, crop oil, acetone, methyl ethyl ketone, cyclohexanone, trichloroethylene, perchloroethylene, ethyl acetate, amyl acetate, butyl acetate, propylene glycol monomethyl ether and diethylene glycol monomethyl ether, methanol, ethanol, isopropanol, amyl alcohol, ethylene glycol, propylene glycol, glycerol, N-methyl-2-pyrrolidone, N,N-dimethylalkylamide, and dimethyl sulfoxide.

[0102] Solid carriers that can be used in the compositions of the present invention may include, but are not limited to, attapulgite, pyrophyllite clay, silica, kaolin, diatomaceous earth, chalk, diatomaceous earth, lime, calcium carbonate, bentonite, Fuller's earth, talc, cottonseed hulls, wheat flour, soybean flour, pumice, wood flour, walnut shell flour, lignin, cellulose, and the like.

[0103] Other exemplary additives that can be used in the compositions provided herein include, but are not limited to, at least one compatibilizer, defoaming agent, chelating agent, neutralizing agent and buffering agent, corrosion inhibitor, coloring agent, flavoring agent, diffusing agent, penetration aid, fixative, dispersing agent, thickening agent, freezing point depression agent, antimicrobial agent, etc.

[0104] In another aspect, the method may further comprise administering at least one adjuvant or at least one safener.

[0105] According to the present invention, the method of controlling target weeds can be carried out by spraying the suggested tank mix, or various herbicides can be formulated into a kit containing various components, which can be mixed according to the instructions before spraying.

[0106] The herbicidal composition of the present invention can be used for targeted crops, such as corn, rice, wheat, barley, rye, oats, sorghum, cotton, soybean, peanut, buckwheat, beet, rapeseed, sunflower, sugarcane, tobacco, etc.; vegetables: Solanaceae vegetables, such as eggplant, tomato, sweet pepper, pepper, potato, etc., melon vegetables, such as cucumber, pumpkin, zucchini, watermelon, melon, pumpkin, etc., cruciferous vegetables, such as radish, white radish, horseradish, kohlrabi, Chinese cabbage, cabbage, mustard, broccoli, cauliflower, etc., Asteraceae vegetables such as burdock, chrysanthemum , artichoke, lettuce, etc., Liliaceae vegetables such as scallions, onions, garlic and asparagus, Umbelliferae vegetables such as carrots, parsley, celery, parsnips, etc., Chenopodiaceae vegetables such as spinach, chard, etc., Lamiaceae vegetables such as perilla, mint, basil, etc., strawberry, sweet potato, yam, taro, etc., flowers, foliage plants, lawn grass, Fruits: pome fruits such as apples, pears, quinces, etc., Nuclear meat fruits such as peaches, plums, nectarines, plums, cherries, apricots, prunes, citrus fruits such as oranges, lemons, rime, grapefruit, etc., nuts. For example, chestnuts, walnuts, hazelnuts, almonds, pistachios, cashews, macadamia nuts, etc., berries, such as blueberries, cranberries, blackberries, raspberries, etc., vines, persimmons, olives, plums, bananas, oil palms, coffee, date palms, coconuts, etc., trees other than fruit trees; tea, mulberry, flowering plants, weeds in trees, such as ash, birch, dogwood, eucalyptus, ginkgo, lilac, maple, oak, poplar, Bauhinia, Liquidambar, sycamore, beech, Japanese cypress, fir, hemlock, juniper, pine, spruce and northeastern yew, etc.

[0107] The target weeds are selected from grass weeds.

[0108] The grass weeds are selected from the group including: barnyard grass, foxtail grass, big foxtail grass, golden foxtail grass, foxtail grass, ciliated crabgrass, blood red crabgrass, flat-branched crabgrass, island crabgrass, goosegrass, bluegrass, wheat field foxtail, big spike foxtail, wild oats, false sorghum, common sorghum, ryegrass, multiflora ryegrass, perennial ryegrass, rigid ryegrass, rye-shaped brome, dry brome, awn barley grass, cylindrical goat grass , reed grass, small-seeded grass, Apira, foreign wild millet, Texas millet, big millet, broad-leaved brachio, Congo brachio, plantain brachio, pitching brachio, signal brachio, humus brachio, prickly tribulus, few-flowered tribulus, wild millet, pasture grass, African tiger grass, teff, red grass, dragon claw grass, field duckbill grass, rice, Baja paspalum, sea paspalum, ground grass, pasture grass or barrel-axis grass.

[0109] Preferably, the target weed is Goosegrass.

[0110] In each of these embodiments, the embodiments may include applying a preferred glutamine synthase herbicide in addition to glyphosate.

[0111] A method of controlling goosegrass weeds according to the present invention, wherein the method comprises applying a herbicide combination simultaneously or subsequently or sequentially to target the weeds.

[0112] In one embodiment, adult plants of Ornithogalum that are resistant to herbicides such as glyphosate and ACCase inhibitors (oxazolidinone, fluazifop-butyl, and clethodim) can be effectively controlled by sequentially applying glyphosate and applying glufosinate 10 to 15 days later to target the herbicide-resistant weeds.

[0113] Thus, in one embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0114] (a) applying glyphosate to the target site; and

[0115] (b) applying glufosinate to the locus within about 10 to about 15 days.

[0116] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0117] (a) applying glyphosate to the locus; and

[0118] (b) applying glufosinate to the locus, wherein the glufosinate is applied at least 7 days after the application of glyphosate.

[0119] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0120] (a) applying glyphosate to the locus; and

[0121] (b) applying glufosinate to the locus, wherein the glufosinate is applied at least 14 days after the application of glyphosate.

[0122] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0123] (a) applying glyphosate to the locus; and

[0124] (b) applying glufosinate to the locus, wherein the glufosinate is applied at least 21 days after the application of glyphosate.

[0125] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0126] (a) applying glyphosate to the locus; and

[0127] (b) applying glufosinate to the locus, wherein the glufosinate is applied at least 28 days after the application of glyphosate.

[0128] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0129] (a) applying glyphosate to the locus; and

[0130] (b) applying glufosinate to the locus, wherein the glufosinate is applied at least 35 days after the application of glyphosate.

[0131] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0132] (a) applying glyphosate to the locus; and

[0133] (b) applying glufosinate to the locus, wherein the glufosinate is applied at least 42 days after the application of glyphosate.

[0134] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0135] (a) applying glyphosate to the locus; and

[0136] (b) applying glufosinate to the locus, wherein the glufosinate is applied at least 49 days after the application of glyphosate.

[0137] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0138] (a) applying glyphosate to the locus; and

[0139] (b) applying glufosinate to the locus, wherein the glufosinate is applied at least 56 days after the application of glyphosate.

[0140] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0141] (a) applying to the locus a combination comprising glyphosate and glufosinate; and

[0142] (b) applying to the locus a combination comprising glyphosate and glufosinate, wherein the second application is applied at least 7 days after the first application.

[0143] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0144] (a) applying to the locus a combination comprising glyphosate and glufosinate; and

[0145] (b) applying to the locus a combination comprising glyphosate and glufosinate, wherein the second application is applied at least 14 days after the first application.

[0146] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0147] (a) applying to the locus a combination comprising glyphosate and glufosinate; and

[0148] (b) applying to the locus a combination comprising glyphosate and glufosinate, wherein the second application is applied at least 21 days after the first application.

[0149] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0150] (a) applying to the locus a combination comprising glyphosate and glufosinate; and

[0151] (b) applying to the locus a combination comprising glyphosate and glufosinate, wherein the second application is applied at least 28 days after the first application.

[0152] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0153] (a) applying to the locus a combination comprising glyphosate and glufosinate; and

[0154] (b) applying to the locus a combination comprising glyphosate and glufosinate, wherein the second application is applied at least 35 days after the first application.

[0155] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0156] (a) applying to the locus a combination comprising glyphosate and glufosinate; and

[0157] (b) applying to the locus a combination comprising glyphosate and glufosinate, wherein the second application is applied at least 42 days after the first application.

[0158] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0159] (a) applying to the locus a combination comprising glyphosate and glufosinate; and

[0160] (b) applying to the locus a combination comprising glyphosate and glufosinate, wherein the second application is applied at least 49 days after the first application.

[0161] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0162] (a) applying to the locus a combination comprising glyphosate and glufosinate; and

[0163] (b) applying to the locus a combination comprising glyphosate and glufosinate, wherein the second application is applied at least 56 days after the first application.

[0164] In one embodiment, glyphosate herbicide is applied at 750 g / kg with nicosulfuron.

[0165] In one embodiment, glyphosate herbicide is applied at 240 g / kg with nicosulfuron.

[0166] In another aspect, the present invention provides a method of controlling herbicide resistant weeds such that the method can be applied pre-emergence or post-emergence of the weeds, thereby providing resistance management and complete control of the weeds.

[0167] Thus, the present invention provides a method for managing goosegrass that is resistant to glyphosate and ACCase inhibitors as well as other herbicides, the method comprising sequentially applying a combination of herbicides.

[0168] Preferably, the target weed is Digitaria tangutica

[0169] In each of these embodiments, the embodiments may include applying a preferred glutamine synthase herbicide and an ACCase inhibitor in addition to glyphosate.

[0170] The method for controlling crabgrass weeds according to the present invention comprises applying a herbicide combination simultaneously or subsequently or sequentially to target the weeds.

[0171] In one embodiment, adult plants of Crabgrass that are resistant to herbicides, such as glyphosate, can be effectively controlled by sequentially applying a glutamine synthase inhibitor and applying a glutamine synthase inhibitor and / or an ACCase inhibitor 7 to 60 days later to target the herbicide resistant weeds.

[0172] In one embodiment, adult plants of Crabgrass that are resistant to herbicides such as glyphosate can be effectively controlled by sequentially applying glufosinate and / or an ACCase inhibitor and applying glufosinate and / or an ACCase inhibitor 7 to 60 days later to target the herbicide resistant weeds.

[0173] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0174] (a) applying glufosinate and / or an ACCase inhibitor herbicide to the site; and

[0175] (b) applying glufosinate and / or an ACCase inhibitor herbicide to the locus, wherein the glufosinate is applied at least 7 days after the first application.

[0176] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0177] (a) applying glufosinate and / or an ACCase inhibitor herbicide to the site; and

[0178] (b) applying glufosinate and / or an ACCase inhibitor herbicide to the locus, wherein the glufosinate is applied at least 14 days after the first application.

[0179] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0180] (a) applying glufosinate and / or an ACCase inhibitor herbicide to the site; and

[0181] (b) applying glufosinate and / or an ACCase inhibitor herbicide to the locus, wherein the glufosinate is applied at least 21 days after the first application.

[0182] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0183] (a) applying glufosinate and / or an ACCase inhibitor herbicide to the site; and

[0184] (b) applying glufosinate and / or an ACCase inhibitor herbicide to the locus, wherein the glufosinate is applied at least 28 days after the first application.

[0185] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0186] (a) applying glufosinate and / or an ACCase inhibitor herbicide to the site; and

[0187] (b) applying glufosinate and / or an ACCase inhibitor herbicide to the locus, wherein the glufosinate is applied at least 35 days after the first application.

[0188] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0189] (a) applying glufosinate and / or an ACCase inhibitor herbicide to the site; and

[0190] (b) applying glufosinate and / or an ACCase inhibitor herbicide to the locus, wherein the glufosinate is applied at least 42 days after the first application.

[0191] In another embodiment, the present invention provides a method of controlling weeds at a location, the method comprising:

[0192] (a) applying glufosinate and / or an ACCase inhibitor herbicide to the site; and

[0193] (b) applying glufosinate and / or an ACCase inhibitor herbicide to the locus, wherein the glufosinate is applied at least 58 days after the first application.

[0194] In another aspect, the methods of the present invention provide effective control of herbicide-resistant weeds, such that the methods can be applied pre- or post-emergence of weeds, thereby providing resistance management and overall control of weeds.

[0195] In one aspect, the compositions of the present invention may be in the form of multiple packages of herbicidal products, or as a kit for treating targeted weeds.

[0196] In one aspect, the invention provides a method for controlling herbicide-resistant weeds by applying an effective amount of a combination of herbicides to a locus of herbicide-resistant plants simultaneously, subsequently or sequentially.

[0197] Thus, the present invention provides a method of controlling weeds, thereby providing resistance management and synergistic control of weeds. The present invention also provides a suitable method to achieve complete weed control, increase yield, and simultaneously target resistance management.

[0198] The present invention will now be described in more detail with reference to the following examples. Although the foregoing written description of the present invention enables those of ordinary skill to make and use what is currently considered to be its best mode, those of ordinary skill will understand and appreciate the existence of variations, combinations and equivalents of the specific embodiments, methods and examples herein. Therefore, the present invention should not be limited to the above-described embodiments, methods and the following examples, but is limited to all embodiments and methods within the scope and spirit of the present invention.

[0199] Examples

[0200] Example 1

[0201] Trial data used to evaluate the management of goosegrass weeds

[0202] Tests were conducted to evaluate the effect of the herbicidal combination on the target weed, Goosegrass. The application effect of the herbicide according to the present invention on the growth of unwelcome plants resistant to herbicides is demonstrated by the following greenhouse experiments. The damage to unwelcome weeds caused by the present composition was evaluated using a scale of 0 to 100% compared to untreated control plants. Here, 0 means no damage, and 100 means that the plant is completely destroyed. The coverage percentage of each weed at time t=0 and time t=T was recorded, and the control percentage was calculated based on the observed weed coverage percentage data. In the following experiment, the herbicidal activity of a single herbicidal composition (applied alone and in combination) was evaluated until 21 days after treatment.

[0203] In this test, time T is the application time after the weeds have emerged and the tested herbicide has been applied, A-0 days, B-7 days, C-14 days and D-21 days (spray rate 150 L / ha). Application "A" is the condition when the weed height is about 30-40 cm. The final value reported is the average of five (5) replicates. The tested herbicides come from readily available formulations, i.e., Glyphotal TR (glyphosate, 480 g ai / L) and Fascinate (glufosinate, 200 g ai / L).

[0204] Therefore, in one embodiment, the present invention also relates to a method of controlling weeds at a location, the method comprising applying glyphosate to the location, and applying glufosinate on the seventh day after applying glyphosate.

[0205] Therefore, in one embodiment, the present invention also relates to a method of controlling weeds at a location, the method comprising applying glyphosate 480g / L formulation to the location at a rate of 3.0L / ha, and applying glufosinate 200g / L formulation at a rate of 3.0L / ha on the seventh day after applying glyphosate.

[0206] Therefore, in one embodiment, the present invention also relates to a method of controlling weeds at a location, the method comprising applying glyphosate to the location, and applying glufosinate on the fourteenth day after applying glyphosate.

[0207] Therefore, in one embodiment, the present invention also relates to a method of controlling weeds at a location, the method comprising applying a glyphosate 480 g / L formulation to the location at a rate of 3.0 L / ha, and applying a glufosinate 200 g / L formulation at a rate of 3.0 L / ha on the fourteenth day after applying the glyphosate.

[0208] Therefore, in one embodiment, the present invention also relates to a method of controlling weeds at a location, the method comprising applying glyphosate to the location, and applying glufosinate on the twenty-first day after applying glyphosate.

[0209] Therefore, in one embodiment, the present invention also relates to a method of controlling weeds at a location, the method comprising applying a glyphosate 480 g / L formulation to the location at a rate of 3.0 L / ha, and applying a glufosinate 200 g / L formulation at a rate of 3.0 L / ha on the twenty-first day after applying glyphosate.

[0210] Therefore, in one embodiment, the present invention also relates to a method of controlling weeds at a location, the method comprising applying a combination of glyphosate and glufosinate to the location, and applying the combination of glyphosate and glufosinate on the fourteenth day after the first application.

[0211] Therefore, in one embodiment, the present invention also relates to a method of controlling weeds at a location, the method comprising applying to the location a glyphosate 480 g / L formulation at a rate of 3.0 L / ha and a glufosinate 200 g / L formulation at a rate of 3.0 L / ha, and applying a glyphosate 480 g / L formulation at a rate of 3.0 L / ha and a glufosinate 200 g / L formulation at a rate of 3.0 L / ha on the fourteenth day after the first application.

[0212] Table 1:

[0213]

[0214] The results are listed below:

[0215] Table 2

[0216]

[0217] The mean values ​​after the same letter were not significantly different (p<0.05) by Tukey's test. Coefficient of variation (CV) % = 12.4

[0218] Comparing the periods of application of glyphosate (first application) and sequential application of glufosinate (second application), the longest period between them, i.e. 21 DAA 'A' showed better weed control, followed by the immediate period, i.e. 14 DAA of 'A', and finally the shortest period, i.e. 7 DAA of 'A'.

[0219] It was observed that a single application of a tank mix of glyphosate and glufosinate at application 'A' and their sequential application at 14 DAA of 'A' showed better control and also showed no regrowth of goosegrass plants.

[0220] Therefore, it can be concluded that the present method of controlling herbicide resistant weeds and the combinations as discussed in the above table surprisingly synergize to control Goosegrass adult weeds.

[0221] Example 2

[0222] Experimental data used to evaluate the management of island crabgrass weeds

[0223] A test was conducted to evaluate the effect of the herbicidal combination on the target weed Crabgrass. The effect of the application of the herbicide on the growth of undesirable herbicide-resistant plants was demonstrated. The damage to the undesirable weeds in the flowering stage caused by the method was evaluated using a scale of 0 to 100% compared to untreated control plants. Here, 0 means no damage, while 100 means that the plants are completely destroyed.

[0224] The percentage cover of each weed at time t=0 and t=T was recorded and the percentage control was calculated from the observed percentage cover data of the weeds.In the following experiments, the herbicidal activity of the individual herbicidal compositions (applied singly and in combination) was assessed up to 21 days after treatment.

[0225] In this test, time T is the application time, where A1-0 day, i.e. the first application time, the sequential second application of the herbicide is carried out after a time interval of 58 days starting from A1, so A2 represents 58 days after the first application (A1), B-14 days after the second application (14DAA2), C-21 days after the second application (21DAA2), D-28 days after the second application (28DAA2) and E-42 days after the second application (42DAA2). The herbicide combination was applied after the weeds emerged at a spray rate of 150L / ha.

[0226] The final value reported is the average of four (4) replicates. The herbicides tested were derived from readily available formulations, namely Glyphotal TR (glyphosate, 480 g ai / L Fascinate (glufosinate, 200 g ai / L) and UPL 340 (clethodim and fluazifop-p-butyl (250+200 g ai / L)).

[0227] In one embodiment, the present invention provides a method for controlling sea crabgrass at a location, the method comprising applying glufosinate to the location, and applying clethodim and fluazifop-butyl on the 58th day after applying glufosinate.

[0228] Therefore, in one embodiment, the present invention provides a method for controlling sea crabgrass at a location, the method comprising applying 200 g / L glufosinate formulation to the location at a rate of 2.5 L / ha, and applying 250 g / L clethodim and 200 g / L fluazifop-butyl formulation at a rate of 0.4 L / ha on the fifty-eighth day after applying glufosinate.

[0229] In one embodiment, the present invention provides a method for controlling sea crabgrass at a location, the method comprising applying a combination of glufosinate, clethodim, and fluazifop-butyl to the location, and applying the combination of glufosinate, clethodim, and fluazifop-butyl to the location on the 58th day after the first application.

[0230] Therefore, in one embodiment, the present invention provides a method of controlling sea crabgrass at a location, the method comprising applying to the location a 200 g / L glufosinate formulation at a rate of 2.5 L / ha and a combination of 250 g / L clethodim and 200 g / L fluazifop-butyl formulation at a rate of 0.4 L / ha; and on the fifty-eighth day after the first application, applying to the location a 200 g / L glufosinate formulation at a rate of 2.5 L / ha and a combination of 250 g / L clethodim and 200 g / L fluazifop-butyl formulation at a rate of 0.4 L / ha.

[0231] Table 3:

[0232]

[0233] Table 4(i): % control of Crabgrass after application 1

[0234]

[0235] Table 4(ii): % control of Crabgrass after application 2 (58DAA1)

[0236]

[0237] It was observed that comparing the control of weeds during the period of application of glufosinate (first application) and the sequential application of clethodim and fluazifop-butyl (second application of 58DAA1), durations of 14 days, 21 days, 28 days and 42 days after the second application showed exemplary control of weeds, with % control reaching up to 93% control.

[0238] It was observed that applying a tank mix of glufosinate + clethodim and haloxyfop-butyl at application 'A1' and sequentially applying the tank mix at A2 (ie 58DAA1) showed enhanced control of Crabgrass.

[0239] Advantages of the present invention:

[0240] The present invention provides a method for effectively controlling herbicide-resistant weeds.

[0241] The present invention provides improved methods for controlling the growth of herbicide-resistant weeds and solves related problems such as poor herbicide control and phytotoxicity.

[0242] The present invention provides a method for controlling herbicide-resistant weeds, wherein the method does not adversely affect the normal growth of useful plants, especially crop plants growing in the vicinity of or in the environment of the weeds.

[0243] The present invention provides an effective preventive and therapeutic method for controlling herbicide-resistant weeds that is superior to conventional methods.

Claims

1. A method for controlling herbicide-resistant weeds by applying a combination of herbicides to a locus simultaneously or sequentially, comprising: i. a first application, which comprises applying glyphosate to the site, ii. a second application comprising applying glufosinate-ammonium to the site, wherein the herbicide-resistant weed is goosegrass, and wherein the second administration is administered 7 days after the first administration.

2. A method for controlling herbicide-resistant weeds by applying a combination of herbicides to a locus simultaneously or subsequently or sequentially, comprising: i. a first application comprising applying glyphosate and glufosinate to the site, ii. a second application comprising applying glyphosate and glufosinate to the site, wherein the herbicide-resistant weed is goosegrass, and wherein the second administration is administered 7 days after the first administration.

3. A method for controlling herbicide-resistant weeds by applying a combination of herbicides to a locus simultaneously or sequentially, comprising: i. a first application comprising applying glufosinate and / or acetyl-CoA carboxylase inhibiting herbicide to the site, ii. a second application comprising applying glufosinate and / or an acetyl-CoA carboxylase inhibiting herbicide to the site, wherein the acetyl-CoA carboxylase inhibiting herbicide is selected from fluazifop-butyl, clethodim or a mixture thereof, wherein the herbicide-resistant weed is Crabgrass, and wherein the second administration is administered 7 days after the first administration.

4. The method of any one of claims 1-3, wherein the second administration is administered 14 days after the first administration.

5. The method of any one of claims 1-3, wherein the second administration is administered 35 days after the first administration.

6. The method according to any one of claims 1 to 3, wherein the herbicide is applied at an amount of 5 g / ha to 500 g / ha.

7. The method of any one of claims 1-3, wherein the herbicide combination of the first application and the second application is applied at a rate of 0.1 to 10 L / ha.

8. The method of any one of claims 1-3, wherein the herbicide combination of the first application and the second application is applied at a rate of 0.5 to 8 L / ha.

9. The method of any one of claims 1-3, wherein the herbicide combination of the first application and the second application is applied at a rate of 1 to 5 L / ha.

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