A hormone combination for improving rapeseed's ability to resist waterlogging and its application

Abstract

The invention discloses a hormone combination for improving the waterlogging resistance of rapeseed, wherein the hormone combination is prepared by mixing 1mmol salicylic acid and 16mL / L indolebutyric acid. The beneficial effect of the invention is that by using a hormone combination of salicylic acid and indolebutyric acid of a certain concentration on rapeseed before and after waterlogging, it is possible to effectively prevent rapeseed from waterlogging disasters and achieve recovery after waterlogging disasters.

Description

Technical Field

[0001] The invention relates to the technical field of rapeseed planting techniques, and in particular to a hormone combination for improving rapeseed's ability to resist waterlogging and an application thereof. Background Art

[0002] Rapeseed [Brassica campestris] is a plant of the cruciferous family. my country is a major producer of rapeseed, accounting for 1 / 3 of the world's area and output. The main rapeseed production areas are distributed in the Yangtze River Basin and 17 provinces (autonomous regions) such as Qinghai, Heilongjiang, and Xinjiang. Among them, Hubei, Anhui, Sichuan, Hunan, and Jiangsu are the main rapeseed planting areas. Due to climatic factors and geographical environment, rapeseed in these areas is prone to waterlogging disasters, resulting in large-scale rapeseed production reductions. Therefore, exploring an economical, convenient, and widely promoted waterlogging control method is an urgent problem to be solved in the development of the rapeseed industry.

[0003] The existing development of waterlogging-tolerant varieties requires a long period of time and has obvious geographical restrictions. Field agronomic measures are time-consuming and labor-intensive, which seriously affects the development of the rapeseed industry and causes waste of resources and manpower costs. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention aims to provide a hormone combination for improving rapeseed's resistance to waterlogging and its application. By applying the hormone combination of the present invention, rapeseed waterlogging disasters can be effectively prevented, and after a waterlogging disaster, the hormone combination of the present invention can be used to effectively restore rapeseed.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] A hormone combination for improving the waterlogging resistance of rapeseed, wherein the hormone combination is prepared by mixing 1mmol salicylic acid and 16mL / L indolebutyric acid.

[0007] As an application of the present invention, a method for effectively preventing rapeseed from waterlogging disasters by using a hormone combination that improves rapeseed's resistance to waterlogging comprises spraying the hormone combination once from the late flowering stage to the silique stage of rapeseed, and spraying is performed in the morning or afternoon on a sunny day; wherein the spraying time is 3 days before waterlogging.

[0008] As another application of the present invention, a method for recovering rapeseed from waterlogging disasters uses a hormone combination that improves rapeseed's resistance to waterlogging. The hormone combination is sprayed once from the end of rapeseed's flowering period to the silique period, and the spraying is carried out in the morning or afternoon on a sunny day; wherein the spraying time is 5 days after the waterlogging.

[0009] The beneficial effect of the present invention is that by using a hormone combination of salicylic acid and indolebutyric acid at a certain concentration on rapeseed before and after waterlogging, it is possible to effectively prevent rapeseed from waterlogging disasters and achieve recovery after rapeseed from waterlogging disasters. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 This is the result diagram of the effects of salicylic acid and indolebutyric acid on the agronomic traits of the rapeseed variety "Xiangyou 420" in preventing the early waterlogging disaster of rapeseed siliques;

[0011] Figure 2 This is the result diagram of the effect of salicylic acid and indolebutyric acid on the yield traits and oil content of the rapeseed variety "Xiangyou 420" in preventing the early waterlogging disaster of rapeseed siliques;

[0012] Figure 3 This is the result of the effect of salicylic acid and indolebutyric acid on SOD and POD of the rapeseed variety "Xiangyou 420" in preventing the early waterlogging disaster of rapeseed siliques;

[0013] Figure 4 This is the result of the effects of salicylic acid and indolebutyric acid on the agronomic traits of the rapeseed variety "Xiangyou 420" after the initial waterlogging of rapeseed siliques;

[0014] Figure 5 This is the result of the effects of salicylic acid and indolebutyric acid on the yield traits and oil content of the rapeseed variety "Xiangyou 420" after the initial waterlogging of rapeseed siliques;

[0015] Figure 6 This figure shows the effects of salicylic acid and indolebutyric acid on SOD and POD of the rapeseed variety "Xiangyou 420" during the recovery of rapeseed siliques after the initial waterlogging disaster. DETAILED DESCRIPTION

[0016] The present invention will be further described below. It should be noted that this embodiment is based on the technical solution and provides a detailed implementation method and a specific operation process, but the protection scope of the present invention is not limited to this embodiment.

[0017] The invention discloses a hormone combination for rape waterlogging. The hormone combination is prepared by mixing 1mmol salicylic acid and 16mL / L indolebutyric acid.

[0018] As an application of the present invention, a method for preventing rapeseed from waterlogging disasters is provided by using a hormone combination that improves rapeseed's resistance to waterlogging. The hormone combination is sprayed once from the end of rapeseed's flowering period to the silique period, and the spraying is performed in the morning or afternoon on a sunny day; wherein the spraying time is 3 days before the waterlogging.

[0019] As another application of the present invention, a method for recovering rapeseed from waterlogging disasters uses a hormone combination that improves rapeseed's resistance to waterlogging. The hormone combination is sprayed once from the end of rapeseed's flowering period to the silique period, and the spraying is carried out in the morning or afternoon on a sunny day; wherein the spraying time is 5 days after the waterlogging.

[0020] Example 1

[0021] This example is used to prove that the hormone combination of the present invention can effectively prevent rapeseed waterlogging disasters.

[0022] 1. Test materials

[0023] The test variety was Xiangyou 420, and the experiment was conducted in the ramie resource garden of the Yunyuan base of Hunan Agricultural University from October 2020 to May 2021 and October 2021 to May 2022. Rapeseed was transplanted in the seedling stage in November 2020, and 5g of fertilizer (N:P:K=15:15:15), 1g of urea, and 4g of superphosphate were applied before transplanting. The experiment set up three treatments: CK1 (waterlogging), CK2 (normal development, no waterlogging), and hormone combination treatment before waterlogging (T1: 1mmol+16mL / L, T2: 2mmol+16mL / L, T3: 4mmol+16mL / L), with 5 replicates. In 2021 and 2022, the aboveground part of rapeseed was first sprayed with salicylic acid and indolebutyric acid for 3 days from the end of flowering to the silique stage (March 15-April 25), and then waterlogged for 5 days. The plants were harvested and sampled in May. The drugs were prepared and used immediately. In the morning or afternoon of a sunny day, the prepared drugs were sprayed on the aboveground part of the rapeseed plants with a hand-pressure spray bottle.

[0024] 2. Measurement items and methods

[0025] Plant height and stem diameter: measured in the field using a tape measure and a vernier caliper.

[0026] Number of yellow leaves and number of first branches: measured during field statistics.

[0027] Thousand-grain weight: electronic automatic grain counter.

[0028] Silique number: 15 siliques were taken from each plant to calculate the average value.

[0029] Oil content: measured using a near-infrared component analyzer.

[0030] Grain weight: measured using a thousandth scale after harvest.

[0031] The formula for calculating the degree of waterlogging is: RIR = (WW(waterlogging)) / W(waterlogging) × 100; where RIR is the relative moisture damage index, W(waterlogging) is the measured value of a certain indicator of spraying clean water, and W is the parallel observation value of the same indicator waterlogging treatment.

[0032] Rapeseed grain composition: analyzed using a near-infrared composition analyzer.

[0033] SOD enzyme: SOD enzyme kit, microplate reader.

[0034] POD enzyme: POD enzyme kit, microplate reader.

[0035] 3. Test results

[0036] Effects of salicylic acid and indolebutyric acid on agronomic and physiological traits of rapeseed siliques to prevent early waterlogging damage

[0037] from Figure 1-3 It can be seen that compared with CK1 and CK2, spraying a combination of 1mmol+16mL / L salicylic acid and indolebutyric acid hormones has a certain increase in agronomic traits such as rapeseed plant height and stem thickness within two years; at the same time, it reduces the impact of waterlogging stress on rapeseed silique weight, number of silique grains, thousand-grain weight and other indicators, and improves the impact of waterlogging stress on the oil content of rapeseed grains to a certain extent; it increases the activity of rapeseed SOD enzyme and POD enzyme and significantly increases rapeseed yield.

[0038] In summary, it is more appropriate to choose a combination of 1mmol salicylic acid and 16mL / L indolebutyric acid hormones. That is, spray 1mmol salicylic acid + 16mL / L indolebutyric acid once from the end of rapeseed flowering to the silique stage (3 days before waterlogging), and spray in the morning or afternoon on a sunny day.

[0039] Example 2

[0040] This example is used to prove that the hormone combination of the present invention can effectively achieve the recovery of rapeseed after waterlogging disaster.

[0041] 1. Test materials

[0042] The test variety was Xiangyou 420. The experiment was conducted in the ramie resource garden of the Yunyuan base of Hunan Agricultural University from October 2020 to May 2021 and October 2021 to May 2022. Rapeseed was transplanted in the seedling stage in November 2020, and 5g of fertilizer (N:P:K=15:15:15), 1g of urea, and 4g of superphosphate were applied before transplanting. The experiment set up three treatments: CK1 (waterlogging), CK2 (normal development, no waterlogging), and hormone combination treatment before waterlogging (T1: 1mmol+16mL / L, T2: 2mmol+16mL / L, T3: 4mmol+16mL / L), with 5 replicates. In 2021 and 2022, the aboveground part of rapeseed was first waterlogged for 5 days from the end of rapeseed flowering to the silique stage (March 15-April 25) and then sprayed. It was harvested and sampled in May. The medicine should be prepared and used immediately. In the morning or afternoon of a sunny day, use a hand-pressure spray bottle to spray the prepared medicine on the above-ground part of the rapeseed plants.

[0043] 2. Measurement items and methods

[0044] Plant height and stem diameter: measured in the field using a tape measure and a vernier caliper.

[0045] Number of yellow leaves and number of first branches: measured during field statistics.

[0046] Thousand-grain weight: electronic automatic grain counter.

[0047] Silique number: 15 siliques were taken from each plant to calculate the average value.

[0048] Oil content: measured using a near-infrared component analyzer.

[0049] Grain weight: measured using a thousandth scale after harvest.

[0050] The formula for calculating the degree of waterlogging is: RIR = (WW(waterlogging)) / W(waterlogging) × 100; where RIR is the relative moisture damage index, W(waterlogging) is the measured value of a certain indicator of spraying clean water, and W is the parallel observation value of the same indicator waterlogging treatment.

[0051] Rapeseed grain composition: analyzed using a near-infrared composition analyzer.

[0052] SOD enzyme: SOD enzyme kit, microplate reader.

[0053] POD enzyme: POD enzyme kit, microplate reader.

[0054] 3. Test results

[0055] Effects of salicylic acid and indolebutyric acid on agronomic and physiological traits of rapeseed siliques after initial waterlogging

[0056] from Figure 4-6 It can be seen that compared with CK1 and CK2, spraying 1mmol+16mL / L salicylic acid and indolebutyric acid hormone combination, two-year data showed that it can slightly increase rapeseed plant height, stem thickness and other indicators; at the same time, it can restore the reduction in rapeseed silique weight, silique grain number, thousand-grain weight and other indicators caused by waterlogging stress, and to a certain extent restore the effect of waterlogging stress on the oil content of rapeseed grains; improve the activity of rapeseed SOD enzyme and POD enzyme and increase rapeseed yield.

[0057] In summary, it is more appropriate to choose a combination of 1mmol salicylic acid and 16mL / L indolebutyric acid hormones. That is, spray 1mmol salicylic acid + 16mL / L indolebutyric acid once from the end of rapeseed flowering to the silique stage (5 days after waterlogging), and spray in the morning or afternoon on a sunny day.

[0058] For those skilled in the art, various corresponding changes and modifications can be made according to the above technical solutions and concepts, and all of these changes and modifications should be included in the protection scope of the claims of the present invention.

Claims

1. A hormone combination for improving rapeseed's ability to resist waterlogging, characterized in that: The hormone combination is prepared by mixing 1 mmol of salicylic acid and 16 mL / L of indolebutyric acid.

2. A method for preventing rapeseed from waterlogging using the hormone combination for improving rapeseed's ability to resist waterlogging as claimed in claim 1, characterized in that: The hormone combination is sprayed once from the end of rapeseed flowering to the silique stage, in the morning or afternoon of a sunny day; the spraying time is 3 days before waterlogging.

3. A method for recovering rapeseed from waterlogging using the hormone combination for improving rapeseed's resistance to waterlogging as claimed in claim 1, characterized in that: The hormone combination is sprayed once from the end of rapeseed flowering to the silique stage, in the morning or afternoon of a sunny day; the spraying time is 5 days after waterlogging.

Images