A method for preparing magnetic fluorescent gel particles driven by controllable magnetic field

Abstract

A method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field, wherein the microfluidic chip used includes a controllable magnetic field generating device consisting of a conductive coil and a magnetic core, and a glass substrate, on which a microfluidic system with a specific microfluidic structure is bonded; the conductive coil and the magnetic core generate a controllable magnetic field under the principle of electromagnetic induction, and magnetic droplets are deflected from a continuous phase solution into a laminar phase solution under the drive of the controllable magnetic field, and the solution inside the magnetic droplets is cross-linked under the action of a cross-linking agent in the laminar phase solution to generate spherical and non-spherical magnetic fluorescent gel particles; the present invention integrates a magnetic field generating device with a conductive coil and a magnetic core, and can regulate the force of the magnetic field by the size of an input electrical signal, thereby achieving precise control of the magnetic droplets; the present invention avoids the problem of gel reaction between the continuous phase and the dispersed phase, which leads to flow channel blockage and contamination, by designing a parallel laminar structure.

Description

Technical Field

[0001] The present invention relates to the field of preparation of magnetic fluorescent gel particles, and in particular to a method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field. Background Art

[0002] Due to their excellent porous structure and biocompatibility, hydrogel microparticles have been applied in biomedical applications, including protein encapsulation, drug release, cell culture, and fluorescent tracers. Traditional methods for preparing hydrogel microspheres include emulsion technology, photolithography, electrohydrodynamics, and mechanical disruption. However, these methods are limited in controlling the size and morphology of hydrogel microspheres, and further improvements are needed in producing monodisperse and uniform hydrogel microspheres or particles.

[0003] In recent years, droplet microfluidics technology has been maturely applied to the synthesis of droplet-based materials. Microparticles and microspheres have been prepared based on the droplet generation method of microfluidic chips with different flow channel structures. This method has unique and significant advantages in the size controllability of microparticles and microspheres. Compared with traditional microsphere preparation methods, microfluidic chips have the advantages of simple preparation process, good controllability, good monodispersity of microspheres, high microsphere yield, low material toxicity, and environmental protection. Droplet microfluidics provides a new solution for the preparation of various morphologically controllable gel microspheres or microparticles. The preparation of hydrogel microspheres and particles usually includes two processes: droplet generation and droplet crosslinking. By controlling the process of droplet generation, the final shape and size of hydrogel microspheres and particles can be controlled.

[0004] However, with the demand for complex functional gel microspheres in biochemical applications, it is often necessary to add crosslinkers to the dispersed phase and the continuous phase, and mix two or more reagents to produce a crosslinking reaction or a chemical reaction. When the catalyst, crosslinker and other reagents are dispersed in the continuous phase and the dispersed phase, it is inevitable that the continuous phase and the dispersed phase will undergo a gelation reaction, which will lead to problems such as flow channel blockage and contamination. Therefore, it is necessary to further develop methods for preparing functional gel microspheres or microparticles to solve the above problems. Summary of the Invention

[0005] In order to solve the above problems and improve the preparation ability of magnetic fluorescent gel particles, the present invention provides a method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field. Magnetic microdroplets are first prepared through a microfluidic chip, and then driven by a controllable magnetic field to induce the magnetic droplets to enter a parallel laminar phase. Under the action of a cross-linking agent in the laminar phase, the gelation of the magnetic polymer droplets is achieved. This invention patent can effectively avoid the problem of direct cross-linking reaction between the continuous phase and the dispersed phase in the flow channel, and is used for the preparation of polyacrylamide fluorescent magnetic microspheres and calcium alginate magnetic gel particles, providing a new idea for the generation of hydrogel microspheres and microparticles.

[0006] The object of the present invention is achieved in the following manner:

[0007] A method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field comprises the following steps:

[0008] A1 configuration of the continuous phase solution, the dispersed phase solution, the laminar phase solution, and the configured solution were loaded into the continuous phase solution reservoir bottle, the dispersed phase solution reservoir bottle, the laminar phase solution reservoir bottle;

[0009] A2. Connect the continuous phase solution reservoir, dispersed phase solution reservoir, and laminar flow phase solution reservoir to nitrogen pressure syringe pumps, adjust the input pressure at each inlet, and use a microfluidic chip to prepare primary magnetic fluorescent gel microspheres or particles.

[0010] A3. Place the primary magnetic fluorescent gel microspheres or particles at room temperature for more than 6 hours to obtain magnetic fluorescent gel microspheres or particles.

[0011] Furthermore, the specific operation of the process of configuring each solution in A1 is as follows: first, take a certain amount of electronic fluorinated oil, add 2% of the mass of electronic fluorinated oil Pico-Surf TIM 2. Shake well to obtain a continuous phase solution for use; weigh the fluorescent reagent fluorescein isothiocyanate dextran, acrylamide, N,N-methylenebisacrylamide, ammonium persulfate and other solid reagents in proportion and mix them into the water-based magnetic fluid, and put them into a magnetic stirring pot. Use a magnetic stirring bar to stir for 30 minutes to fully dissolve the solid reagents, and then obtain a dispersed phase solution for use; then take a certain amount of electronic fluorinated oil and add 0.5% of the mass of the electronic fluorinated oil Pico-Surf TIM 2 and 1% cross-linking agent tetramethylethylenediamine, and then a laminar phase solution is obtained for use; wherein the dispersed phase solution contains 1 mg / ml fluorescent reagent fluorescein isothiocyanate dextran (FITC-DEXTRAN, MW = 5000), 50% by mass of acrylamide, 1% N,N-methylenebisacrylamide and 1% ammonium persulfate water-based magnetic fluid.

[0012] Furthermore, the specific operations of the process of preparing each solution in A1 are as follows: first, a certain amount of hexadecane is taken, Span 80 (3% by mass of hexadecane) is added and shaken to obtain a continuous phase solution for use; 1.1% by mass of sodium alginate and 1 mg / ml of fluorescent reagent fluorescein isothiocyanate dextran (FITC-DEXTRAN, MW = 5000) are added to the water-based magnetic fluid, mixed evenly, and placed in a magnetic stirring pot, stirred with a magnetic stirring bar for 20 minutes to fully dissolve the solid reagent, and then a dispersed phase solution is obtained for use; 2% by mass of calcium chloride and 1% polyether (F127) are added to deionized water, placed in a magnetic stirring pot, stirred with a magnetic stirring bar for 20 minutes to fully dissolve the solid reagent, and then a laminar phase solution is obtained for use.

[0013] Furthermore, the microfluidic chip includes a glass substrate 2 and a controllable magnetic field generating device located on the surface thereof, the controllable magnetic field generating device is composed of a conductive coil 13 and a magnetic core 12, and a microfluidic system 1 with a specific microfluidic structure is bonded to the glass substrate 2; the microfluidic system 1 includes a continuous phase flow channel 5, a dispersed phase flow channel 6 and a laminar phase flow channel 10, the inlet end of the continuous phase flow channel 5 is a continuous phase inlet 3, and the end of the continuous phase flow channel 5 is connected to the intermediate flow channel 18, the inlet end of the dispersed phase flow channel 6 is a dispersed phase inlet 8, and the end of the dispersed phase flow channel 6 is connected to the intermediate flow channel 18, the inlet end of the laminar phase flow channel 11 is a laminar phase inlet 10, and the end of the laminar phase flow channel 11 is connected to the intermediate flow channel 18; the continuous phase inlet 3 is connected to one end of the first delivery hose 4, and the other end of the first delivery hose 4 is connected to the continuous phase inlet 3. The microfluidic system 1 is connected to a phase solution storage bottle, the dispersed phase inlet 8 is connected to one end of the second delivery hose 7, the other end of the second delivery hose 7 is connected to the dispersed phase solution storage bottle, the laminar phase inlet 10 is connected to one end of the third delivery hose 9, the other end of the third delivery hose 9 is connected to the laminar phase solution storage bottle, the continuous phase solution storage bottle, the dispersed phase solution storage bottle, and the laminar phase solution storage bottle are all connected to a nitrogen pressure injection pump, and a gel particle outlet 14 and a waste liquid outlet 17 are provided at the end of the intermediate flow channel 18, the gel particle outlet 14 is connected to one end of the fourth delivery hose 15, and the other end of the fourth delivery hose 15 is connected to a collection container, the waste liquid outlet 17 is connected to one end of the fifth delivery hose 16, and the other end of the fifth delivery hose 16 is connected to a waste liquid container; the material of the microfluidic system 1 is polydimethylsiloxane.

[0014] Furthermore, the specific operations for preparing primary magnetic fluorescent gel particles in step A2 are as follows: connecting the continuous phase solution storage bottle, the dispersed phase solution storage bottle, and the laminar phase solution storage bottle to the nitrogen pressure injection pump respectively; turning on the nitrogen pressure injection pump, pressing the continuous phase solution in the continuous phase solution storage bottle into the continuous phase flow channel 5 through the first delivery hose 4, pressing the dispersed phase solution in the dispersed phase solution storage bottle into the dispersed phase flow channel 6 through the second delivery hose 7, and pressing the laminar phase solution in the laminar phase solution storage bottle into the laminar phase flow channel 10 through the third delivery hose 9; adjusting the input pressure of each inlet so that The dispersed phase solution in the dispersed phase flow channel 6 quickly generates uniform magnetic microdroplets under the fluid shear of the continuous phase solution flowing at high speed in the continuous phase flow channel 5, so that the continuous phase solution and the laminar phase solution form parallel laminar flows in the intermediate flow channel 18; the conductive coil 13 is energized, and the conductive coil 13 and the magnetic core 12 generate a controllable magnetic field. Driven by the controllable magnetic field, the magnetic droplets are deflected from the continuous phase solution into the laminar phase solution, and the solution inside the magnetic droplets is cross-linked under the action of the cross-linking agent in the laminar phase solution, and is collected after passing through the fourth conveying hose 15 to obtain primary magnetic fluorescent gel microspheres.

[0015] Compared with the prior art, the present invention has the following technical effects:

[0016] (1) The present invention integrates the magnetic field into a micro-droplet generation chip with a parallel laminar flow structure, realizing the simple preparation of spherical and non-spherical magnetic fluorescent gel particles.

[0017] (2) The present invention integrates a conductive coil and a magnetic core to generate a magnetic field, which can adjust the force of the magnetic field by adjusting the size of the input electrical signal, thereby achieving precise control of the magnetic droplets.

[0018] (3) The present invention can avoid the problem of flow channel blockage and contamination by designing a parallel laminar flow structure, which occurs when the continuous phase and the dispersed phase undergo gelation reaction. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic structural diagram of the microfluidic chip device in the present invention.

[0020] Figure 2 It is a schematic diagram of the preparation process of the magnetic fluorescent gel particles of the present invention.

[0021] Figure 3 This is an experimental diagram of the magnetic droplet generation process of the present invention.

[0022] Figure 4 These are experimental images of the magnetic droplet of the present invention in the absence of a magnetic field (upper image) and in the presence of a magnetic field (lower image).

[0023] Figure 5 These are the optical image (left) and fluorescent image (right) of the polyacrylamide magnetic fluorescent gel microspheres prepared in the present invention.

[0024] Figure 6 These are the optical image (left) and fluorescent image (right) of the calcium alginate fluorescent gel particles prepared by the present invention. DETAILED DESCRIPTION

[0025] The following will be combined with the accompanying drawings to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] A method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field, such as Figure 1-2As shown, the preparation method is based on a microfluidic chip, which includes a glass substrate 2 and a controllable magnetic field generating device located on its surface, wherein the controlled magnetic field generating device is composed of a conductive coil 13 and a magnetic core 12, and a microfluidic system 1 with a specific microfluidic structure is bonded to the glass substrate 2; the microfluidic system 1 includes a continuous phase flow channel 5, a dispersed phase flow channel 6 and a laminar phase flow channel 10, the inlet end of the continuous phase flow channel 5 is a continuous phase inlet 3, and the end of the continuous phase flow channel 5 is connected to the intermediate flow channel 18, the inlet end of the dispersed phase flow channel 6 is a dispersed phase inlet 8, and the end of the dispersed phase flow channel 6 is connected to the intermediate flow channel 18, the inlet end of the laminar phase flow channel 11 is a laminar phase inlet 10, and the end of the laminar phase flow channel 11 is connected to the intermediate flow channel 18; the continuous phase inlet 3 is connected to one end of the first delivery hose 4, and the first delivery hose 4 is connected to the continuous phase solution storage bottle, the dispersed phase inlet 8 is connected to one end of the second delivery hose 7, the other end of the second delivery hose 7 is connected to the dispersed phase solution storage bottle, the laminar phase inlet 10 is connected to one end of the third delivery hose 9, the other end of the third delivery hose 9 is connected to the laminar phase solution storage bottle, the continuous phase solution storage bottle, the dispersed phase solution storage bottle, and the laminar phase solution storage bottle are all connected to a nitrogen pressure injection pump, the end of the intermediate flow channel 18 is provided with a gel particle outlet 14 and a waste liquid outlet 17, the gel particle outlet 14 is connected to one end of the fourth delivery hose 15, the other end of the fourth delivery hose 15 is connected to a collection container, the waste liquid outlet 17 is connected to one end of the fifth delivery hose 16, and the other end of the fifth delivery hose 16 is connected to a waste liquid container; the material of the microfluidic system 1 is polydimethylsiloxane.

[0027] Example 1

[0028] Taking the preparation of polyacrylamide magnetic fluorescent gel microspheres as an example, the preparation method includes the following steps:

[0029] First, take a certain amount of electronic fluorinated oil and add 2% of the mass of the electronic fluorinated oil to the surfactant (Pico-Surf TIM 2) Shake well for later use; weigh the solid reagents such as fluorescein isothiocyanate dextran, acrylamide, N,N-methylenebisacrylamide, and ammonium persulfate in proportion and mix them into the water-based magnetic fluid. Place the mixture in a magnetic stirring pot and stir with a magnetic stirring bar for 30 minutes to fully dissolve the solid reagents. Then, obtain a dispersed phase solution for later use; take a certain amount of electronic fluorinated oil and add a surfactant (Pico-Surf) at a concentration of 2% by weight of the electronic fluorinated oil. TIM2) and 1% cross-linking agent tetramethylethylenediamine, and then obtain a laminar phase solution for use; connect the continuous phase solution storage bottle, the dispersed phase solution storage bottle, and the laminar phase solution storage bottle to the nitrogen pressure injection pump respectively; turn on the nitrogen pressure injection pump, adjust the input pressure of each inlet, press the continuous phase solution of the continuous phase solution storage bottle into the continuous phase flow channel 5 through the first delivery hose 4 and the continuous phase inlet 3 and fill the continuous phase flow channel 5, press the dispersed phase solution of the dispersed phase solution storage bottle into the dispersed phase flow channel 6 through the second delivery hose 7 and the dispersed phase inlet 8 and fill the dispersed phase flow channel 6, and press the laminar phase solution of the laminar phase solution storage bottle into the laminar phase flow channel 11 through the third delivery hose 9 and the laminar phase inlet 10 and fill the laminar phase flow channel 11; adjust the input pressure of each inlet so that the dispersed phase solution in the dispersed phase flow channel 6 quickly generates uniform magnetic microdroplets under the fluid shear of the high-speed continuous phase solution flowing in the continuous phase flow channel 5 ( Figure 2 , Figure 3 ), so that the continuous phase solution and the laminar phase solution form parallel laminar flows in the middle flow channel 18 ( Figure 2 ), there is a clear interface between the parallel laminar flows; Figure 4 As shown, the conductive coil 13 is energized, and the conductive coil 13 and the magnetic core 12 generate a controllable magnetic field. Driven by the controllable magnetic field, the magnetic droplets are deflected from the continuous phase solution into the laminar phase solution. The solution inside the magnetic droplets is cross-linked under the action of the cross-linking agent in the laminar phase solution and is collected after passing through the fourth conveying hose 15 to obtain primary magnetic fluorescent gel microspheres; the primary magnetic fluorescent gel microspheres are placed at room temperature for more than 6 hours to obtain magnetic fluorescent gel microspheres or particles, whose optical images and fluorescent images are shown as follows. Figure 5 shown.

[0030] Example 2

[0031] Taking the preparation of sodium alginate fluorescent gel microparticles as an example, the preparation method includes the following steps:

[0032] First, a certain amount of hexadecane was taken, and a surfactant (Span 80) with a mass fraction of 3% of hexadecane was added and shaken well for later use; sodium alginate with a mass fraction of 1.1% and a fluorescent reagent fluorescein isothiocyanate dextran (FITC-DEXTRAN, MW = 1.00) were added to the water-based magnetic fluid. 5000) and put it into a magnetic stirring pot, stir it with a magnetic stirring bar for 20 minutes to fully dissolve the solid reagent, and then obtain a dispersed phase solution for use; add 2% by mass of calcium chloride and 1% polyether (F127) into deionized water, put it into a magnetic stirring pot, stir it with a magnetic stirring bar for 20 minutes to fully dissolve the solid reagent, and then obtain a laminar phase solution for use; connect the continuous phase solution storage bottle, the dispersed phase solution storage bottle, and the laminar phase solution storage bottle to the nitrogen pressure injection pump respectively; turn on the nitrogen pressure injection pump, adjust the input pressure of each inlet, press the continuous phase solution of the continuous phase solution storage bottle into the continuous phase flow channel 5 through the first delivery hose 4 and the continuous phase inlet 3 and fill it, press the dispersed phase solution of the dispersed phase solution storage bottle into the dispersed phase flow channel 6 through the second delivery hose 7 and the dispersed phase inlet 8 and fill it, and connect the laminar phase solution storage bottle to the laminar phase solution storage bottle. The fluid phase solution is pressed into and fills the laminar phase flow channel 11 through the third delivery hose 9 and the laminar phase inlet 10; the input pressure of each inlet is adjusted so that the dispersed phase solution in the dispersed phase flow channel 6 quickly generates uniform magnetic microdroplets under the fluid shear of the continuous phase solution flowing at high speed in the continuous phase flow channel 5, so that the continuous phase solution and the laminar phase solution form parallel laminar flows in the intermediate flow channel 18, and there is a clear interface between the parallel laminar flows; the conductive coil 13 is energized, and the conductive coil 13 and the magnetic core 12 generate a controllable magnetic field. Driven by the controllable magnetic field, the magnetic droplets are deflected from the continuous phase solution into the laminar phase solution. The solution inside the magnetic droplets is cross-linked under the action of the laminar phase solution and is collected after passing through the fourth delivery hose 15 to obtain primary magnetic fluorescent gel particles; the primary magnetic fluorescent gel microspheres are placed at room temperature for more than 6 hours to obtain magnetic fluorescent gel particles, whose optical images and fluorescent images are as shown in FIG. Figure 6 shown.

[0033] The above implementation methods are only used to illustrate the embodiments of the present invention, and are not intended to limit the embodiments of the present invention. Ordinary technicians in the relevant technical field may make various changes and modifications without departing from the spirit and scope of the embodiments of the present invention. Therefore, all equivalent technical solutions also fall within the scope of the embodiments of the present invention, and the scope of patent protection of the embodiments of the present invention should be defined by the claims.

Claims

1. A method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field, characterized in that: The following steps are involved: A1 configuration of the continuous phase solution, the dispersed phase solution, the laminar phase solution, and the configured solution were loaded into the continuous phase solution reservoir bottle, the dispersed phase solution reservoir bottle, the laminar phase solution reservoir bottle; The specific operation of the process of configuring each solution in A1 is as follows: first, take a certain amount of electronic fluorinated oil, add 2% of the mass of electronic fluorinated oil Pico-Surf TIM 2. Shake well to obtain a continuous phase solution for use; weigh the fluorescent reagent fluorescein isothiocyanate dextran, acrylamide, N,N-methylenebisacrylamide, and ammonium persulfate solid reagent in proportion and mix them into the water-based magnetic fluid, and put them into a magnetic stirring pot. Use a magnetic stirring bar to stir for 30 minutes to fully dissolve the solid reagents, and then obtain a dispersed phase solution for use; then take a certain amount of electronic fluorinated oil and add 0.5% of the mass of the electronic fluorinated oil Pico-Surf TIM 2% and 1% cross-linking agent tetramethylethylenediamine, and then a laminar phase solution is obtained for use; wherein the dispersed phase solution contains 1 mg / ml fluorescent reagent fluorescein isothiocyanate dextran, 50% by mass of acrylamide, 1% N,N-methylenebisacrylamide and 1% ammonium persulfate water-based magnetic fluid; A2. Connect the continuous phase solution reservoir, dispersed phase solution reservoir, and laminar phase solution reservoir to a nitrogen pressure injection pump, respectively. Adjust the input pressure at each inlet so that the dispersed phase solution in the dispersed phase channel is sheared by the high-speed continuous phase solution flowing in the continuous phase channel, rapidly generating uniform magnetic microdroplets. This allows the continuous phase solution and laminar phase solution to form parallel laminar flows in the intermediate channel. Power is supplied to the conductive coil, which, together with the magnetic core, generates a controllable magnetic field. Driven by the controllable magnetic field, the magnetic droplets are deflected from the continuous phase solution into the laminar phase solution. The solution within the magnetic droplets is then cross-linked by the crosslinker in the laminar phase solution and collected through a fourth delivery hose, yielding primary magnetic fluorescent gel microspheres. A3. Place the primary magnetic fluorescent gel microspheres or particles at room temperature for more than 6 hours to obtain magnetic fluorescent gel microspheres or particles.

2. The method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field according to claim 1, wherein: The specific operations of the process of preparing each solution in A1 are as follows: first, a certain amount of hexadecane is taken, Span 80 (3% by mass of hexadecane) is added and shaken to obtain a continuous phase solution for use; 1.1% by mass of sodium alginate and 1 mg / ml of the fluorescent reagent fluorescein isothiocyanate dextran are added to the water-based magnetic fluid, the mixture is mixed, and the mixture is placed in a magnetic stirring pot and stirred with a magnetic stirrer for 20 minutes to fully dissolve the solid reagent, thereby obtaining a dispersed phase solution for use; 2% by mass of calcium chloride and 1% of polyether are added to deionized water, the mixture is placed in a magnetic stirring pot and stirred with a magnetic stirrer for 20 minutes to fully dissolve the solid reagent, thereby obtaining a laminar phase solution for use.

3. The method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field according to claim 1, wherein: The microfluidic chip includes a glass substrate (2) and a controllable magnetic field generating device located on the surface thereof, wherein the controllable magnetic field generating device is composed of a conductive coil (13) and a magnetic core (12). A microfluidic system (1) having a specific microfluidic structure is bonded to the glass substrate (2); the microfluidic system (1) includes a continuous phase flow channel (5), a dispersed phase flow channel (6) and a laminar phase flow channel (10); the inlet end of the continuous phase flow channel (5) is a continuous phase inlet (3), and the end of the continuous phase flow channel (5) is connected to the intermediate flow channel (18); the inlet end of the dispersed phase flow channel (6) is a dispersed phase inlet (8), and the end of the dispersed phase flow channel (6) is connected to the intermediate flow channel (18); the inlet end of the laminar phase flow channel (11) is a laminar phase inlet (10), and the end of the laminar phase flow channel (11) is connected to the intermediate flow channel (18); the continuous phase inlet (3) is connected to one end of a first delivery hose (4), and the other end of the first delivery hose (4) is connected to the laminar phase inlet (10). The end of the intermediate flow channel (18) is connected to the continuous phase solution storage bottle, the dispersed phase inlet (8) is connected to one end of the second delivery hose (7), the other end of the second delivery hose (7) is connected to the dispersed phase solution storage bottle, the laminar phase inlet (10) is connected to one end of the third delivery hose (9), the other end of the third delivery hose (9) is connected to the laminar phase solution storage bottle, the continuous phase solution storage bottle, the dispersed phase solution storage bottle, and the laminar phase solution storage bottle are all connected to the nitrogen pressure injection pump, the end of the intermediate flow channel (18) is provided with a gel particle outlet (14) and a waste liquid outlet (17), the gel particle outlet (14) is connected to one end of the fourth delivery hose (15), the other end of the fourth delivery hose (15) is connected to the collection container, the waste liquid outlet (17) is connected to one end of the fifth delivery hose (16), and the other end of the fifth delivery hose (16) is connected to the waste liquid container; the material of the microfluidic system (1) is polydimethylsiloxane.

4. A method for preparing magnetic fluorescent gel particles driven by a controllable magnetic field according to any one of claims 1 to 3, characterized in that: The specific operation of preparing primary magnetic fluorescent gel particles in step A2 is as follows: connecting the continuous phase solution storage bottle, the dispersed phase solution storage bottle, and the laminar phase solution storage bottle to the nitrogen pressure injection pump respectively; turning on the nitrogen pressure injection pump, pressing the continuous phase solution in the continuous phase solution storage bottle into the continuous phase flow channel (5) through the first delivery hose (4), pressing the dispersed phase solution in the dispersed phase solution storage bottle into the dispersed phase flow channel (6) through the second delivery hose (7), and pressing the laminar phase solution in the laminar phase solution storage bottle into the laminar phase flow channel (10) through the third delivery hose (9), adjusting the input pressure of each inlet so that the dispersed phase solution in the continuous phase solution storage bottle is pressed into the continuous phase flow channel (5) through the first delivery hose (4), pressing the dispersed phase solution in the dispersed phase solution storage bottle into the dispersed phase flow channel (6) through the second delivery hose (7), and pressing the laminar phase solution in the laminar phase solution storage bottle into the laminar phase flow channel (10) through the third delivery hose (9), and adjusting the input pressure of each inlet so that the dispersed phase solution in the continuous phase solution storage bottle is pressed into the dispersed phase flow channel (5) through the second delivery hose (7). The dispersed phase solution in the flow channel (6) quickly generates uniform magnetic microdroplets under the fluid shear of the continuous phase solution flowing at a high speed in the continuous phase flow channel (5), so that the continuous phase solution and the laminar phase solution form a parallel laminar flow in the middle flow channel (18); the conductive coil (13) is energized, and the conductive coil (13) and the magnetic core (12) generate a controllable magnetic field. Driven by the controllable magnetic field, the magnetic droplets are deflected from the continuous phase solution into the laminar phase solution, and the solution inside the magnetic droplets is cross-linked under the action of the cross-linking agent in the laminar phase solution, and is collected after passing through the fourth conveying hose (15) to obtain primary magnetic fluorescent gel microspheres.

Images