Multi-channel rotary microfluidic immunoassay chip

Abstract

The application relates to the microfluidic technology field and provides a multi-channel rotary microfluidic immunodetection chip, which comprises a chip body, a sample inlet channel, a gas outlet, a waste liquid pool, a liquid return channel and at least two spaced detection units arranged on the chip body; the waste liquid pool is in communication with the detection units, the liquid return channel and the gas outlet respectively; the chip body is further provided with at least two spaced sample storage channels; a rotary assembly is rotatably arranged on the chip body; the rotary assembly is provided with a first connecting channel, an air inlet channel and a second connecting channel for constructing a communication path. The application has the effects of reducing the flow pollution of samples and improving the detection accuracy.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microfluidic technology, in particular to a multi-channel rotary microfluidic immunoassay chip. BACKGROUND

[0002] Microfluidic technology can integrate sample preparation, reaction, separation and other basic operation units in the biochemical analysis process on a chip to automatically complete the analysis process, which has the advantages of less sample consumption, fast detection speed and simple operation, and is often used in marker detection in the medical field to realize the diagnosis of related diseases.

[0003] The existing microfluidic detection chip adopts a single-piece or multi-channel mode. When a single piece is used for detection, the detection efficiency is low, and the sample is wasted. The multi-channel mode is the mainstream detection mode. The main process is that the sample to be tested enters the chip from the sample inlet, flows along the microchannel inlet in the length direction of the main flow channel under the guidance of the driving part, and is guided to each microchannel and flows to the waste pool under the driving of the driving part. When a single microchannel is transported, corresponding air holes and suction ends need to be set to ensure the passage. Multiple detection units need to be set with multiple air holes for one-to-one communication. After the sample to be tested in the sample to be tested reacts with the substance in the detection unit, the sample to be tested is scanned and detected by an external scanner, and then flows into the waste pool for treatment.

[0004] However, in the multi-channel mode, the sample flow needs to be driven for detection. The structure of the ordinary multi-channel chip is complex, and the sample flow is easy to contact with too much air, which pollutes the sample flow and reduces the accuracy of the detection result. SUMMARY

[0005] In order to improve the problem that the existing sample is easy to be polluted by air flow and the accuracy of the detection result is reduced, the present application provides a multi-channel rotary microfluidic immunoassay chip.

[0006] The multi-channel rotary microfluidic immunoassay chip provided by the present application adopts the following technical scheme:

[0007] A multi-channel rotary microfluidic immunoassay chip, comprising a chip main body, wherein the chip main body is provided with a sample inlet channel, an air outlet, a waste pool, a liquid return channel and at least two spaced detection units; the waste pool is in communication with the detection units, the liquid return channel and the air outlet, characterized in that it further comprises a rotating assembly rotatably arranged on the chip main body.

[0008] The chip body is further provided with at least two spaced sample storage channels, the rotating assembly is provided with a first connecting channel, an air inlet channel and a second connecting channel for constructing a communication path; the sample inlet channel, the liquid return channel and the at least two sample storage channels are communicated through the first connecting channel to form a first communication path, the air inlet channel, the first connecting channel and the second connecting channel are rotated by a preset distance along with the rotating assembly, so that the first communication path is disconnected, and the air inlet channel, the sample storage channel and the detection unit are communicated through the second connecting channel to form a second communication path.

[0009] By adopting the above technical scheme, after the first connecting channel on the rotating assembly communicates the first communication path, the air outlet is exhausted by the external driving member, so that the liquid sample in the sample inlet channel enters the first communication path and is temporarily stored in all the sample storage channels. The rotating assembly is rotated, the first connecting channel is disconnected from the first communication path, and the second connecting channel communicates the second communication path, so that the air inlet channel is communicated with the corresponding sample storage channel. The second connecting channel communicates the corresponding sample storage channel with the liquid inlet of the corresponding detection unit. The external driving member exhausts the air outlet to form a negative pressure, so that the liquid sample in the sample storage channel flows into the detection unit through the second connecting channel for detection. After detection, the rotating assembly can be continuously rotated to realize one-by-one delivery and distribution of the liquid sample in the sample storage channel. Only a single air inlet channel is needed to realize switching of the path, without the need to set multiple air inlet channels, thereby reducing errors and air pollution.

[0010] Optionally, when the sample storage channel and the detection unit are provided as two, the sample inlet channel, the liquid return channel and the two sample storage channels are communicated through the first connecting channel to form a first communication path. The air inlet channel, the first connecting channel and the second connecting channel are rotated by a preset distance along with the rotating assembly, so that the air inlet channel, the second connecting channel communicate with the first sample storage channel, the second communication path is communicated, and the first communication path is disconnected. The air inlet channel, the first connecting channel and the second connecting channel are continuously rotated by a preset distance along with the rotating assembly, so that the air inlet channel, the second connecting channel communicate with the second sample storage channel, the second communication path is communicated, and the first sample storage channel and the first communication path are disconnected.

[0011] By adopting the above technical scheme, when the sample storage channel and the detection unit are provided as two, the air inlet channel and the second connecting channel can be rotated by a preset distance along with the rotating assembly, so that the two sample storage channels and the corresponding detection units can be conveniently switched.

[0012] Optionally, when the sample storage channels and the detection units are three, the sample inlet channel, the liquid return channel and the three sample storage channels are connected through the first connecting channel to form a first communication path, the sample inlet channel, the first connecting channel and the second connecting channel rotate by a preset distance with the rotating assembly, so that the sample inlet channel and the second connecting channel communicate with a first sample storage channel, the second communication path is connected, and the first communication path is disconnected; the sample inlet channel, the first connecting channel and the second connecting channel continue to rotate by a preset distance with the rotating assembly, so that the sample inlet channel and the second connecting channel communicate with a second sample storage channel, the second communication path is connected, and the first sample storage channel and the first communication path are disconnected; the sample inlet channel, the first connecting channel and the second connecting channel continue to rotate by a preset distance with the rotating assembly, so that the sample inlet channel and the second connecting channel communicate with a third sample storage channel, the second communication path is connected, and the first sample storage channel and the second sample storage channel and the first communication path are disconnected.

[0013] By adopting the above technical scheme, when the sample storage channels and the detection units are three, the three corresponding sample storage channels and the detection units can be switched in turn through the sample inlet channel and the second connecting channel on the rotating assembly, and the sample detection can be performed according to the selection of the rotating switching to the specified sample storage channel and the detection unit.

[0014] Optionally, the sample inlet channel, the first connecting channel and the second connecting channel rotate by a preset distance with the rotating assembly, the second connecting channel is not connected to the second communication path, so that the second communication path is disconnected, and the first connecting channel communicates with the first communication path.

[0015] By adopting the above technical scheme, the second connecting channel is not connected to the second communication path, so that at least two sample storage channels are not connected to the second connecting channel, and the liquid sample can be transported to the sample storage channel for storage through the external driving member by the connection of the first connecting channel with the sample storage channel and the liquid return channel.

[0016] Optionally, the sensor is further included, and the number of the sensors is at least three, and at least two detection units and the liquid return channel are provided with the sensors.

[0017] By adopting the above technical scheme, the sensor can detect the amount of the flowing liquid sample in the sample storage channel and the detection unit, when the sensor of the liquid return channel detects the liquid sample, the liquid sample has filled the sample storage channel and reached the liquid return channel, and when the sensor at the end of the detection unit detects the liquid sample, it indicates that the liquid sample in the sample storage channel has completely entered the detection unit, and the on-off of the external driving member can be controlled through the electric signal.

[0018] Optionally, the volumes of the sample storage channels are the same.

[0019] By adopting the above technical scheme, under the first communication path, the sample storage channels are filled, and the amount of liquid sample in each sample storage channel is the same, so that the effect of equal detection is achieved, and the detection accuracy is improved.

[0020] Optionally, the rotating assembly further comprises a movable element, an ejection element, and a rotary valve; the movable element is installed on the chip body; the rotary valve is movably connected with the movable element, and the first connecting channel and the second connecting channel are located in the rotary valve; the ejection element is slidably connected with the chip body and is connected with the movable element.

[0021] By adopting the above technical scheme, the rotary valve is ejected by the ejection element until the connecting ports of the first connecting channel and the second connecting channel are located outside the movable element, the rotary valve is manually pressed against, and the rotary valve is rotated by applying force, so that the liquid sample in the first connecting channel and the second connecting channel is affected by the centrifugal force and flows out of the connecting ports, thereby achieving the effect of conveniently cleaning the first connecting channel and the second connecting channel.

[0022] Optionally, the rotating assembly further comprises a recovery element; the recovery element is connected with the movable element and is located at an end of the movable element away from the chip body.

[0023] By adopting the above technical scheme, when the first connecting channel and the second connecting channel are affected by the centrifugal force, the liquid sample splashes from the connecting ports, and the splashed liquid sample is adhered to the recovery element, thereby avoiding the influence of the splashed liquid sample on the surrounding.

[0024] Optionally, the recovery element is larger than the rotary valve and is provided with a recovery groove.

[0025] By adopting the above technical scheme, the recovery element can wrap the rotary valve, and the recovery groove is used to make the liquid sample adhered to the inner wall of the recovery element fall along the wall surface into the recovery groove, thereby being uniformly recovered and preventing from dripping onto the chip body.

[0026] Optionally, the chip body is further provided with a cleaning element and a cleaning channel in communication with the cleaning element; the cleaning channel is in communication with the sample inlet channel and is connected to the first communication path.

[0027] By adopting the above technical scheme, the sample inlet of the sample inlet channel is covered by the external closure cover, and when the external driving element is pumping, only the cleaning liquid in the cleaning element can be pumped, the rotating assembly switches the first connecting channel to the first communication path, so that the cleaning liquid is pumped into the sample storage channel, and then the rotating assembly switches the second connecting channel to the second communication path, so that the cleaning liquid in the sample storage channel flows to the detection unit for cleaning.

[0028] In summary, the present application includes at least one of the following beneficial technical effects:

[0029] 1、the first connecting channel on the rotating assembly accesses the first communication path, and the external driving member draws air from the air outlet, so that the liquid sample in the sample inlet channel enters the first communication path and is temporarily stored in the sample storage channel. The rotating assembly is rotated, the first connecting channel is disconnected from the first communication path, and the second connecting channel is connected to the second communication path, so that the gas inlet channel is connected to the corresponding sample storage channel. The second connecting channel connects the corresponding sample storage channel to the liquid inlet of the corresponding detection unit. The external driving member draws air from the air outlet to form negative pressure, so that the liquid sample in the sample storage channel flows into the detection unit through the second connecting channel for detection. After the detection is completed, the rotating assembly can be continuously rotated to realize one-by-one delivery and distribution of the liquid sample in the sample storage channel. Only a single gas inlet channel is needed to realize switching of the path, and multiple gas inlet channels are not needed, reducing errors and air pollution;

[0030] 2、the sensor can detect the amount of liquid sample flowing in the sample inlet channel and the detection unit, and the on-off of the external driving member can be controlled through an electrical signal;

[0031] 3、in the first communication path, after each sample storage channel is filled, the amount of liquid sample in each sample storage channel is the same, thereby achieving the effect of equal detection and improving the accuracy of detection;

[0032] 4、the rotating valve is ejected by the ejecting member until the connecting ports of the first connecting channel and the second connecting channel are located outside the movable member. The rotating valve is manually pressed against and rotated by applying force to it. The liquid sample in the first connecting channel and the second connecting channel flows out of the connecting ports under the action of centrifugal force during rotation, thereby facilitating cleaning of the first connecting channel and the second connecting channel;

[0033] 5、the sample inlet of the sample inlet channel is covered by the external closure cover. When the external driving member draws air, only the cleaning liquid in the cleaning member can be drawn through the cleaning channel. The rotating assembly switches the first connecting channel to the first communication path, so that the cleaning liquid is drawn into the sample storage channel. Subsequently, the rotating assembly switches the second connecting channel to the second communication path, so that the cleaning liquid in the sample storage channel flows into the detection unit for cleaning. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 is a first cross-sectional structure diagram of the chip main body in the first communication path of embodiment 1 of the present application;

[0035] Figure 2 is a cross-sectional structure diagram of the rotating assembly in embodiment 1 of the present application;

[0036] Figure 3is a schematic diagram of the cross-sectional structure of the second communication path of the chip main body in Embodiment 1 of the present application;

[0037] Figure 4 is a schematic diagram of the exploded structure of the chip main body in Embodiment 2 of the present application;

[0038] Figure 5 is a schematic diagram of the second cross-sectional structure of the first communication path of the chip main body in Embodiment 3 of the present application;

[0039] The labels in the drawings are: 1, chip main body, 11, sample inlet channel, 12, waste liquid pool, 121, gas outlet, 13, detection unit, 131, reaction pool, 132, scanning pool, 133, reaction channel, 2, sample storage channel, 3, rotating assembly, 31, first connecting channel, 32, second connecting channel, 33, gas inlet channel, 34, movable part, 35, ejector, 36, rotary valve, 37, recovery part, 371, recovery groove, 4, sensor, 5, cleaning part, 6, cleaning channel. DETAILED DESCRIPTION

[0040] The following will be described in detail in combination with the accompanying drawings. Figure 1 - the accompanying drawings Figure 5 , the present application will be further described in detail.

[0041] The embodiments of the present application disclose a multi-channel rotary microfluidic immunodetection chip.

[0042] Embodiment 1:

[0043] A multi-channel rotary microfluidic immunodetection chip, referring to Figure 1 , comprising a chip main body 1, the chip main body 1 is provided with a sample inlet channel 11, a gas outlet 121, a waste liquid pool 12 and at least two spaced detection units 13; the waste liquid pool 12 is respectively communicated with the detection unit 13, the liquid return channel and the gas outlet 121;

[0044] Among them, the chip main body 1 is the main bearing tool for installing and detecting the detection structure, the sample inlet channel 11 includes the sample port provided on the chip main body 1 and opened for the sample to enter, the sample port can be sealed by an external sealing cover, the detection unit 13 provides a reaction and detection place for the liquid sample, the number of detection units 13 is determined according to actual needs, Figure 1 The number shown is three. The waste liquid pool 12 can be respectively communicated with the detection unit 13, the sample inlet channel 11 and the liquid return channel, the gas outlet 121 can be externally connected with a driving part, the driving part can adopt a power source capable of forming negative pressure such as a gas pump or a piston syringe, the waste liquid pool 12 is pumped by the driving part through the gas outlet 121, so that negative pressure is formed in the waste liquid pool 12, the circulation of the liquid sample is facilitated, and the waste liquid discharged from the detection unit 13 is finally collected in the waste liquid pool 12.

[0045] The chip body 1 is provided with a rotating assembly 3 rotatably arranged thereon, the rotating assembly 3 is provided with a connecting channel and an air inlet channel 33 for building a communication path, and the rotating assembly 3 is provided with a first connecting channel 31, the air inlet channel 33 and a second connecting channel 32 for building a communication path; and the chip body 1 is further provided with at least two spaced sample storage channels 2.

[0046] When the sample storage channels 2 and the detection unit 13 are provided with two, the sample inlet channel 11, the liquid return channel and the two sample storage channels 2 are communicated through the first connecting channel 31 to form a first communication path, the first connecting channel 31 is divided into three channels which are communicated with the sample inlet channel 11, the liquid return channel and the two sample storage channels 2 in sequence, the air inlet channel 33, the first connecting channel 31 and the second connecting channel 32 rotate with the rotating assembly 3 by a preset distance, so that the air inlet channel 33 and the second connecting channel 32 communicate with the first sample storage channel 2, the second communication path is communicated, and the first communication path is disconnected; the air inlet channel 33, the first connecting channel 31 and the second connecting channel 32 continue to rotate with the rotating assembly 3 by a preset distance, so that the air inlet channel 33 and the second connecting channel 32 communicate with the second sample storage channel 2, the second communication path is communicated, and the first sample storage channel 2 and the first communication path are disconnected.

[0047] When the sample storage channels 2 and the detection unit 13 are provided with three, the sample inlet channel 11, the liquid return channel and the three sample storage channels 2 are communicated through the first connecting channel 31 to form a first communication path, the first connecting channel 31 is divided into four channels which are communicated with the sample inlet channel 11, the liquid return channel and the three sample storage channels 2 in sequence, the air inlet channel 33, the first connecting channel 31 and the second connecting channel 32 rotate with the rotating assembly 3 by a preset distance, so that the air inlet channel 33 and the second connecting channel 32 communicate with the first sample storage channel 2, the second communication path is communicated, and the first communication path is disconnected; the air inlet channel 33, the first connecting channel 31 and the second connecting channel 32 continue to rotate with the rotating assembly 3 by a preset distance, so that the air inlet channel 33 and the second connecting channel 32 communicate with the second sample storage channel 2, the second communication path is communicated, and the first sample storage channel 2 and the first communication path are disconnected, the air inlet channel 33, the first connecting channel 31 and the second connecting channel 32 continue to rotate with the rotating assembly 3 by a preset distance, so that the air inlet channel 33 and the second connecting channel 32 communicate with the third sample storage channel 2, the second communication path is communicated, and the first sample storage channel 2, the second sample storage channel 2 and the first communication path are disconnected.

[0048] Specifically, the rotating assembly 3 is located on the first communication path and the second communication path, and the first communication path and the second communication path are controlled by rotation through the first connecting channel 31, the air inlet channel 33 and the second connecting channel 32 on the rotating assembly 3; when the first connecting channel 31 is rotated to the sample inlet channel 11 and all the sample storage channels 2 through the rotating action of the rotating assembly 3, the sample inlet channel 11, the sample storage channels 2 and the liquid return channel are communicated with the waste liquid pool 12 to form a first communication path in communication with each other, the second connecting channel 32 is not connected to the second communication path, so that the second communication path is disconnected, air is drawn through the air outlet 121 connected to the external driving member to form a negative pressure in the waste liquid pool 12, and the liquid sample is drawn from the sample port of the sample inlet channel 11 to the sample storage channel 2 for temporary storage.

[0049] Referring to Figure 2 When the second connecting channel 32 and the air inlet channel 33 are rotated by a preset distance to the designated second communication path by applying a rotating force to the rotating assembly 3, the air inlet channel 33 is communicated with the corresponding selected sample storage channel 2, the sample storage channel 2 is communicated with the detection unit 13 through the second connecting channel 32, the second communication path is formed, air is drawn through the air outlet 121 connected to the external driving member to form a negative pressure in the waste liquid pool 12, and the liquid sample in the sample storage channel 2 is drawn to the detection unit 13, and the communication of each path can be realized without multiple air inlet channels 33, thereby reducing errors.

[0050] After the detection of a single detection unit 13 is completed, the rotating assembly 3 can be continuously rotated to switch the second connecting channel 32 and the air inlet channel 33 to the second connecting path corresponding to other detection units 13 for detection, so that a single air inlet channel 33 is switched to each detection unit 13, thereby improving the accuracy of detection.

[0051] The number of the detection units 13 and the sample storage channels 2 is consistent, and at least two of each is provided, and the specific number of the detection units 13 and the sample storage channels 2 is set according to the design requirements of the actual product.

[0052] The number of the detection units 13 and the sample storage channels 2 is consistent, and at least two of each is provided, and the specific number of the detection units 13 and the sample storage channels 2 is set according to the design requirements of the actual product.

[0053] The detection unit 13 comprises a reaction pool 131, a scanning pool 132 and a reaction channel 133; the reaction channel 133 sequentially connects the reaction pool 131, the scanning pool 132 and the waste liquid pool 12, and the scanning pool 132 is connected with the sensing end of the external sensor 4; the driving member is used to draw air to form negative pressure in the waste liquid pool 12; the chemical substance, such as freeze-dried reagent, exists in the reaction pool 131; the reaction channel 133 can adopt a serpentine channel S type or a U type channel, and the embodiment adopts the serpentine channel; after the liquid sample enters the reaction channel 133 under the negative pressure state through the first connecting channel 31, the liquid sample melts the freeze-dried reagent in the reaction pool 131 and mixes and reacts in the serpentine channel; after the liquid sample in the scanning pool 132 is detected by the sensor 4, the external driving member is stopped, the incubation time is counted, the scanning instrument is used to scan the scanning pool 132 to make the liquid sample fully react and obtain the detection result; finally, the liquid sample is driven by the external driving member to enter the waste liquid pool 12 for unified recovery.

[0054] The volume of each sample storage channel 2 is the same, so that the liquid sample in each sample storage channel 2 is the same after each sample storage channel 2 is filled under the first connecting path, so as to achieve the effect of equal detection and improve the detection accuracy.

[0055] The sample inlet channel 11, the first connecting channel 31, the second connecting channel 32, the sample storage channel 2 and the reaction channel 133 can all adopt a pipeline or a cavity; when the chip main body 11 is solid, a cavity can be adopted; when the chip main body 11 is hollow, a pipeline can be adopted.

[0056] The implementation principle of the embodiment 1 of the application comprises the following steps: after the first connecting channel 31 of the rotating assembly 3 is connected with the first connecting path, the air outlet 121 is drawn by the external driving member to make the liquid sample in the sample inlet channel 11 enter the first connecting path and be temporarily stored in all the sample storage channels 2; the rotating assembly 3 is rotated, the first connecting channel 31 is disconnected from the first connecting path, the second connecting channel 32 is connected with the second connecting path, the air inlet channel 33 is connected with the corresponding sample storage channel 2, the second connecting channel 32 connects the corresponding sample storage channel 2 with the liquid inlet of the corresponding detection unit 13, the air outlet 121 is drawn by the external driving member to form negative pressure, the liquid sample in the sample storage channel 2 flows into the detection unit 13 through the second connecting channel 32 for detection; after the detection is completed, the rotating assembly 3 can be continuously rotated to realize one-by-one delivery and distribution of the liquid sample in the sample storage channel 2, and only one air inlet channel 33 is needed to realize the switching path, so that multiple air inlet channels 33 are not needed, and errors and air pollution are reduced.

[0057] Embodiment 2

[0058] Reference Figure 4As shown, the embodiment 2 of the present application is optimized on the basis of the embodiment 1, and the optimization includes that the rotating assembly 3 further comprises a movable piece 34, an ejection piece 35 and a rotating valve 36; the movable piece 34 is installed on the chip body 1; the rotating valve 36 is movably connected with the movable piece 34, and the first connecting channel 31 and the second connecting channel 32 are located in the rotating valve 36; the ejection piece 35 is slidably connected with the chip body 1 and is connected with the movable piece 34 in a socket manner. Since the first connecting channel 31 and the second connecting channel 32 serve as intermediate connecting points, after the liquid sample is transported, the liquid sample will be stored in the first connecting channel 31 and the second connecting channel 32, which results in the inconvenience in cleaning the liquid sample in the first connecting channel 31 and the second connecting channel 32. Therefore, the insertion opening for the ejection piece 35 is formed on the movable piece 34. After the ejection piece 35 is inserted into the insertion opening, the rotating valve 36 is ejected until the connecting openings of the first connecting channel 31 and the second connecting channel 32 are located outside the movable piece 34. Since the rotating valve 36 can rotate along the movable piece 34, the rotating valve 36 can be manually resisted and a force can be applied to the rotating valve 36 to make it rotate along the movable piece 34. During the rotation, the liquid sample in the first connecting channel 31 and the second connecting channel 32 is affected by the centrifugal force and flows out of the connecting openings, so as to conveniently clean the first connecting channel 31 and the second connecting channel 32.

[0059] In the embodiment, the ejection piece 35 can be a wedge-shaped block. When the wedge-shaped block slides along the chip body 1, it can be inserted into the movable piece 34 and eject the rotating valve 36 upward, so that the connecting openings of the first connecting channel 31 and the second connecting channel 32 in the rotating valve 36 are exposed above the movable piece 34. Therefore, the rotating force can be manually applied to the liquid sample in the first connecting channel 31 and the second connecting channel 32 to take out the liquid sample.

[0060] In the embodiment, the rotating assembly 3 further comprises a recovery piece 37; the recovery piece 37 is connected with the movable piece 34 and is located at the end of the movable piece 34 away from the chip body 1. Since the liquid sample in the first connecting channel 31 and the second connecting channel 32 is affected by the centrifugal force and splashes around, which affects the surrounding environment, the recovery piece 37 is arranged on the movable piece 34. The recovery piece 37 has a ring structure and the height of the recovery piece 37 is higher than the position of the first connecting channel 31 and the second connecting channel 32. Therefore, when the first connecting channel 31 and the second connecting channel 32 are affected by the centrifugal force, the liquid sample splashes from the connecting openings and adheres to the recovery piece 37 due to the blockage of the recovery piece 37, so as to avoid the influence of the splashing liquid sample on the surrounding environment.

[0061] The recovery piece 37 is larger than the rotary valve 36 and has a recovery groove 371 inside; the recovery piece 37 is larger in size than the rotary valve 36, so that the recovery piece 37 can wrap the rotary valve 36, and the recovery groove 371 is used to make the liquid sample adhered to the inner wall of the recovery piece 3736 fall along the wall to the recovery groove 371, so as to be uniformly recovered and prevented from dripping to the chip main body 1.

[0062] The connection between the recovery piece 37 and the movable piece 34 is detachable connection; the detachable connection between the recovery piece 37 and the movable piece 34 can be clamping or screwing, and the clamping is taken as an example, a clamping block is arranged on the surface of the recovery piece 37, a clamping groove is arranged on the movable piece 34, the recovery piece 37 is clamped into the clamping groove through the clamping block, so as to be clamped, when the recovery piece 37 needs to be detached, the recovery piece 37 is pulled along the direction of the clamping groove, so that the recovery piece 37 can be separated from the adhered liquid sample.

[0063] The implementation principle of the embodiment 2 of the application includes: when the detection is finished, the liquid sample remaining in the first connecting channel 31 and the second connecting channel 32 is inconvenient to process, so the ejector 35 is moved along the chip main body 11 to the rotary valve 36, and the rotary valve 36 is ejected from the movable piece 34, then force is applied to the rotary valve 36, so that the rotary valve 36 rotates along the movable piece 34, the centrifugal force generated in the rotating process of the rotary valve 36 throws the liquid sample in the first connecting channel 31 and the second connecting channel 32 out, and adheres to the recovery piece 37, the liquid sample flows along the wall of the recovery piece 37 to the recovery groove 371 to be collected, when the collection is finished, the recovery piece 37 is detached from the movable piece 34 to be cleaned, and the ejector 35 is moved along the chip main body 1 to be separated from the rotary valve 36, so that the rotary valve 36 loses support, and then can be pushed back into the movable piece 34, so as to conveniently clean the liquid sample in the first connecting channel 31 and the second connecting channel 32.

[0064] Embodiment 3

[0065] Reference Figure 5As shown, the embodiment 3 of the present application is optimized on the basis of the above-mentioned embodiments, and comprises the following optimization: the chip main body 1 is further provided with a cleaning member 5 and a cleaning channel 6 in communication with the cleaning member 5; the cleaning channel 6 is in communication with the sample inlet channel 11 and is connected to the first communication path; the cleaning member 5 can be a rubber bag or a rubber cover containing cleaning liquid; when the detection is completed, it is very inconvenient to clean each channel in the chip main body 1, so the cleaning member 5 is arranged to assist in cleaning each channel of the chip main body 1 and the rotating assembly 3; the main steps are similar to those of the embodiment; the sample inlet of the sample inlet channel 11 is covered by an external closing cover; the first connecting channel 31 is connected to the first communication path; when the external driving member is pumping, only the cleaning liquid in the cleaning member 5 can be pumped through the cleaning channel 6; the position of the second connecting channel 32 and the air inlet channel 33 is switched by the rotating assembly 3, so that the second connecting channel 32 is connected to the second communication path; the starting and stopping of the external driving member is controlled by the sensor 4, so that the cleaning liquid flows to the sample storage channel 2 and the detection unit 13 in the chip main body 1 along with the rotating assembly 3, and finally flows to the waste liquid pool 12 for unified treatment.

[0066] The implementation principle of the embodiment 3 of the present application comprises: the sample inlet of the sample inlet channel 11 is covered by an external closing cover; when the external driving member is pumping, only the cleaning liquid in the cleaning member 5 can be pumped through the cleaning channel 6; the position of the first connecting channel 31 and the second connecting channel 32 is simultaneously switched by the rotating assembly 3; the starting and stopping of the external driving member is controlled by the sensor 4, so that the cleaning liquid flows to the chip main body 1 along with the rotating assembly 3, and finally flows to the detection unit 13 for unified treatment.

[0067] The embodiments of the specific implementation are the preferred embodiments of the present application, but do not limit the protection scope of the present application; the same parts are denoted by the same reference numerals. Therefore: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A multi-channel rotary microfluidic immunoassay chip, characterized in that, The chip body (1) is provided with a sample inlet channel (11), a gas outlet (121), a waste liquid pool (12), a liquid return channel and at least two spaced detection units (13); the waste liquid pool (12) is in communication with the detection units (13), the liquid return channel and the gas outlet (121) respectively, and further comprises a rotating assembly (3) rotatably arranged on the chip body (1); The chip body (1) is further provided with at least two spaced sample storage channels (2), the rotating assembly (3) is provided with a first connecting channel (31) for building a communication path, an air inlet channel (33) and a second connecting channel (32); the sample inlet channel (11), the liquid return channel and the at least two sample storage channels (2) are in communication through the first connecting channel (31) to form a first communication path, the air inlet channel (33), the first connecting channel (31) and the second connecting channel (32) rotate with the rotating assembly (3) by a preset distance, so that the first communication path is disconnected, and the air inlet channel (33), the at least two sample storage channels (2) and the at least two detection units (13) are in communication through the second connecting channel (32) to form a second communication path; the volumes of the sample storage channels (2) are the same; the rotating assembly (3) further comprises a movable part (34), an ejection part (35) and a rotary valve (36); the movable part (34) is mounted on the chip body (1); the rotary valve (36) is movably connected with the movable part (34), and the first connecting channel (31) and the second connecting channel (32) are located in the rotary valve (36); the ejection part (35) is slidably connected with the chip body (1) and is connected with the movable part (34) in a socket manner; the rotating assembly (3) further comprises a recovery part (37); the recovery part (37) is connected with the movable part (34) and located at an end of the movable part (34) away from the chip body (1); the recovery part (37) is larger than the rotary valve (36) and is provided with a recovery groove (371) therein; the chip body (1) is further provided with a cleaning part (5) and a cleaning channel (6) in communication with the cleaning part (5); the cleaning channel (6) is in communication with the sample inlet channel (11) and is connected to the first communication path.

2. The multi-channel rotary microfluidic immunoassay chip according to claim 1, wherein, When the sample storage channel (2) and the detection unit (13) are provided in two, the sample inlet channel (11), the liquid return channel and the two sample storage channels (2) are connected through the first connecting channel (31) to form a first communication path, the air inlet channel (33), the first connecting channel (31) and the second connecting channel (32) rotate with the rotating assembly (3) by a preset distance, so that the air inlet channel (33) and the second connecting channel (32) are connected to the first sample storage channel (2), the second communication path is connected, and the first communication path is disconnected; the air inlet channel (33), the first connecting channel (31) and the second connecting channel (32) continue to rotate with the rotating assembly (3) by a preset distance, so that the air inlet channel (33) and the second connecting channel (32) are connected to the second sample storage channel (2), the second communication path is connected, and the first sample storage channel (2) and the first communication path are disconnected.

3. The multi-channel rotary microfluidic immunoassay chip according to claim 1, wherein, When the sample storage channel (2) and the detection unit (13) are provided in three, the sample inlet channel (11), the liquid return channel and the three sample storage channels (2) are connected through the first connecting channel (31) to form a first communication path, the air inlet channel (33), the first connecting channel (31) and the second connecting channel (32) rotate with the rotating assembly (3) by a preset distance, so that the air inlet channel (33) and the second connecting channel (32) are connected to the first sample storage channel (2), the second communication path is connected, and the first communication path is disconnected; the air inlet channel (33), the first connecting channel (31) and the second connecting channel (32) continue to rotate with the rotating assembly (3) by a preset distance, so that the air inlet channel (33) and the second connecting channel (32) are connected to the second sample storage channel (2), the second communication path is connected, and the first sample storage channel (2) and the first communication path are disconnected, the air inlet channel (33), the first connecting channel (31) and the second connecting channel (32) continue to rotate with the rotating assembly (3) by a preset distance, so that the air inlet channel (33) and the second connecting channel (32) are connected to the third sample storage channel (2), the second communication path is connected, and the first sample storage channel (2) and the second sample storage channel (2) and the first communication path are disconnected.

4. The multi-channel rotary microfluidic immunoassay chip according to claim 1, wherein, The air inlet channel (33), the first connecting channel (31) and the second connecting channel (32) rotate with the rotating assembly (3) by a preset distance, the second connecting channel (32) is not connected to the second communication path, so that the second communication path is disconnected, and the first connecting channel (31) is connected to the first communication path.

5. The multi-channel rotary microfluidic immunoassay chip according to claim 1, wherein, Further comprising a sensor (4); the number of the sensor (4) is at least three, and the liquid return channel and at least two detection units (13) are provided with the sensor (4).

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