Packaging method for samples used for spectral measurement

Abstract

This invention provides a method for packaging a sample for spectral measurement, comprising: firstly, cleaning a first transparent substrate and a second transparent substrate with deionized water; then, sequentially using deionized water and an organic solvent as cleaning agents and employing an ultrasonic cleaner to ultrasonically clean the first and second transparent substrates; subsequently, drying the first and second transparent substrates with a drying gas; next, taking a sample solution and dropping it onto the center of the first surface of the first transparent substrate, shaking the first transparent substrate to spread the sample solution evenly on the first surface; then, placing the first transparent substrate on a heating stage and heating it to completely evaporate the solvent in the sample solution on the first surface, leaving the solute portion; finally, covering the solute portion of the first transparent substrate with a second transparent substrate to obtain a sample for spectral measurement. The packaging method of this invention is easy to operate, produces a uniform sample distribution, and can be tested in a vacuum environment.

Description

Technical Field

[0001] This invention relates to the field of spectral measurement technology, and in particular to a method for packaging samples for spectral measurement. Background Technology

[0002] Spectroscopic measurement is one of the most commonly used analytical testing methods, widely applied in materials science, life sciences, physics, chemistry, and other fields. With the rapid popularization and application of spectroscopic analysis instruments, the range of test samples is constantly expanding. For spectroscopic testing of powder samples, sample pretreatment is required before placing them on the sample holder of the spectroscopic analysis instrument for spectral analysis. This invention provides a rapid, reliable, and low-cost powder encapsulation technology for powder sample pretreatment, facilitating spectroscopic analysis of powder samples.

[0003] Currently, there are several methods for pretreatment of powder samples for spectral analysis. One method involves placing the powder sample in a specific sample holder, but this method is highly specific and not suitable for general powder samples. It also requires a specially designed sample holder, which is relatively expensive. Another method involves placing the powder sample directly on a quartz plate. However, this method cannot guarantee uniform distribution of the powder sample and cannot be used to test the sample in a vacuum environment. A third method involves pressing the powder sample into a pellet before testing. This method may damage the sample and is not suitable for general powder samples.

[0004] Therefore, existing technologies still need to be improved and developed. Summary of the Invention

[0005] The main objective of this invention is to provide a sample encapsulation method that causes almost no damage to the sample, allows the sample to be uniformly distributed on the substrate, and enables the encapsulated sample to be tested in a vacuum environment.

[0006] This invention provides a method for packaging a sample for spectral measurement, the packaging method comprising:

[0007] Provide a first transparent substrate and a second transparent substrate;

[0008] The first and second light-transmitting substrates were cleaned with deionized water.

[0009] Next, deionized water was used as the cleaning agent and an ultrasonic cleaner was used to ultrasonically clean the first and second transparent substrates.

[0010] Next, an organic solvent was used as a cleaning agent and an ultrasonic cleaner was used to ultrasonically clean the first and second light-transmitting substrates.

[0011] Then, the first and second light-transmitting substrates were dried with a drying gas.

[0012] Next, a sample solution is dropped onto the center of the first surface of the first light-transmitting substrate, and the first light-transmitting substrate is shaken to make the sample solution spread evenly on the first surface of the first light-transmitting substrate;

[0013] The first transparent substrate is then placed on a heating stage with the first surface facing upwards and heated to completely evaporate the solvent in the sample solution on the first surface, leaving the solute portion.

[0014] Then, the second light-transmitting substrate is placed over the solute portion of the first light-transmitting substrate to obtain a sample for spectral measurement.

[0015] In an optional embodiment of the present invention, the method of cleaning the first light-transmitting substrate and the second light-transmitting substrate with deionized water can be rinsing or brushing.

[0016] In an optional embodiment of the present invention, the step of subsequently using deionized water as a cleaning agent and ultrasonically cleaning the first and second light-transmitting substrates using an ultrasonic cleaner includes:

[0017] The first and second transparent substrates, after being cleaned with deionized water, are placed in a beaker containing deionized water. The beaker containing deionized water is then placed in the cleaning tank of an ultrasonic cleaner and subjected to ultrasonic vibration for 10-20 minutes.

[0018] In an optional embodiment of the present invention, the subsequent ultrasonic cleaning of the first and second light-transmitting substrates using an organic solvent as a cleaning agent and an ultrasonic cleaning machine includes:

[0019] First, remove the first and second transparent substrates from the cleaning tank of the ultrasonic cleaner containing deionized water; place the first and second transparent substrates into a beaker containing acetone, and then place the beaker containing acetone into the cleaning tank of the ultrasonic cleaner and ultrasonically vibrate for 10-20 minutes.

[0020] Next, the first and second transparent substrates are removed from the cleaning tank of the ultrasonic cleaner containing acetone; then the first and second transparent substrates are placed in a beaker containing ethanol, and then the beaker containing ethanol is placed in the cleaning tank of the ultrasonic cleaner and ultrasonically vibrated for 10-20 minutes.

[0021] Then, the first and second transparent substrates are removed from the cleaning tank of the ultrasonic cleaner containing ethanol; the first and second transparent substrates are then placed into a beaker containing isopropanol, and the beaker containing isopropanol is then placed into the cleaning tank of the ultrasonic cleaner and ultrasonically vibrated for 10-20 minutes.

[0022] In an optional embodiment of the present invention, the subsequent drying of the first and second light-transmitting substrates with a drying gas includes:

[0023] Remove the first and second transparent substrates from the beaker containing isopropanol;

[0024] The first and second light-transmitting substrates were dried with nitrogen gas.

[0025] In an optional embodiment of the present invention, shaking the first light-transmitting substrate to cause the sample solution to spread evenly on the first surface of the first light-transmitting substrate includes:

[0026] Hold the first light-transmitting substrate with tweezers and tilt the first light-transmitting substrate in various directions so that the sample solution evenly covers the first surface of the first light-transmitting substrate.

[0027] In an optional embodiment of the present invention, the packaging method further includes:

[0028] The spectral measurement sample is attached to the sample holder by using tape around the edge of the second transparent substrate.

[0029] In an optional embodiment of the present invention, the first light-transmitting substrate and the second light-transmitting substrate are quartz substrates, sapphire substrates, silicon oxide substrates or organic thin film substrates.

[0030] Beneficial Effects: This invention provides a method for packaging samples for spectral measurement, comprising: firstly, cleaning a first transparent substrate and a second transparent substrate with deionized water; then, sequentially using deionized water and an organic solvent as cleaning agents and employing an ultrasonic cleaner to ultrasonically clean the first and second transparent substrates; subsequently, drying the first and second transparent substrates with a drying gas; then, taking a sample solution and dropping it onto the center of the first surface of the first transparent substrate, shaking the first transparent substrate to spread the sample solution evenly on the first surface of the first transparent substrate; next, placing the first transparent substrate on a heating stage and heating it to completely evaporate the solvent in the sample solution on the first surface, leaving the solute portion; and finally, covering the solute portion of the first transparent substrate with a second transparent substrate to obtain a sample for spectral measurement. The packaging method of this invention is easy to operate, produces uniform sample distribution, and can be tested in a vacuum environment. Attached Figure Description

[0031] Figure 1 This is a schematic diagram of an embodiment of a sample packaging method for spectral measurement according to the present invention;

[0032] Figure 2 For the encapsulated powder carbon dot sample fixed on the sample holder, after undergoing a vacuuming process (10 - 5 A picture of the actual product (mbar);

[0033] Figure 3 For unencapsulated powder carbon dot samples fixed on a sample holder, after undergoing a vacuum process (10 -5 A picture of the actual product (mbar);

[0034] Figure 4 The image shows a combination of terahertz time-domain spectra obtained under vacuum conditions for an unencapsulated powder carbon dot sample (E1), an encapsulated powder carbon dot sample (E2), and a sample with only a double-layer substrate (E0). Detailed Implementation

[0035] This invention provides a method for packaging samples for spectral measurement. The terms "first," "second," "third," and "fourth" are used in the specification, claims, and accompanying drawings of this invention.

[0036] The terms "etc." (if present) are used to distinguish similar objects, not necessarily to describe a specific order or sequence. It should be understood that such use of data can be interchanged where appropriate, so that the actual objects described herein...

[0037] Examples may be implemented in any order other than those illustrated or described herein. Furthermore, the terms "including" or "having" and any variations thereof are intended to cover non-exclusive inclusion, e.g., including...

[0038] A process, method, system, product, or apparatus consisting of a series of steps or units is not necessarily limited to those steps or units that are explicitly listed, but may include other steps or units that are not explicitly listed or that are inherent to those processes, methods, products, or apparatuses.

[0039] See Figure 1 The present invention provides a method for packaging a sample for spectral measurement, the packaging method comprising:

[0040] S100, providing a first transparent substrate and a second transparent substrate; in optical transmission measurement, both the first transparent substrate and the second transparent substrate are transparent substrates, such as in terahertz spectroscopy measurement, the first transparent substrate and the second transparent substrate are quartz substrates, sapphire substrates, silicon oxide substrates or organic thin film substrates.

[0041] 5S200, Clean the first and second light-transmitting substrates with deionized water;

[0042] The method of cleaning the first and second light-transmitting substrates with deionized water can be rinsing, brushing, or wiping by hand.

[0043] S300, Next, using deionized water as a cleaning agent and an ultrasonic cleaner, the first light-transmitting part is cleaned.

[0044] The first and second transparent substrates are then ultrasonically cleaned; specifically, deionized water is used as the cleaning agent and an ultrasonic cleaner is employed to clean the first and second transparent substrates.

[0045] Ultrasonic cleaning includes: placing the first and second transparent substrates, which have been cleaned with deionized water, into a beaker containing deionized water, and then placing the beaker containing deionized water into the cleaning tank of an ultrasonic cleaner, and ultrasonically vibrating for 10-20 minutes (e.g., 10 minutes, 15 minutes, 20 minutes).

[0046] S400, Next, use an organic solvent as a cleaning agent and use an ultrasonic cleaner to perform ultrasonic cleaning on the first and second light-transmitting substrates; the purpose of using an organic solvent to clean the first and second light-transmitting substrates is to use the principle of like dissolves like to thoroughly remove the organic matter attached to the substrates, so as to ensure the cleanliness of the substrate surface.

[0047] Specifically, the next step of using an organic solvent as a cleaning agent and employing an ultrasonic cleaning machine to perform ultrasonic cleaning on the first and second light-transmitting substrates includes:

[0048] First, remove the first and second transparent substrates from the cleaning tank of an ultrasonic cleaner containing deionized water; place the first and second transparent substrates into a beaker containing acetone, and then place the beaker containing acetone into the cleaning tank of the ultrasonic cleaner and ultrasonically vibrate for 10-20 minutes (e.g., 10 minutes, 15 minutes, 20 minutes).

[0049] Next, the first and second light-transmitting substrates are removed from the cleaning tank of the ultrasonic cleaner containing acetone; then the first and second light-transmitting substrates are placed in a beaker containing ethanol, and then the beaker containing ethanol is placed in the cleaning tank of the ultrasonic cleaner and ultrasonically vibrated for 10-20 minutes (e.g., 10 minutes, 15 minutes, 20 minutes).

[0050] Then, the first and second transparent substrates are removed from the cleaning tank of an ultrasonic cleaner containing ethanol; the first and second transparent substrates are then placed in a beaker containing isopropanol, and the beaker containing isopropanol is then placed in the cleaning tank of the ultrasonic cleaner and ultrasonically vibrated for 10-20 minutes (e.g., 10 minutes, 15 minutes, 20 minutes). It should be noted that the reason why the above-mentioned organic solvents are used to clean the organic matter on the first and second transparent substrates is that the above-mentioned organic solvents can effectively remove organic matter on the surface of the first and second transparent substrates that interferes with the terahertz detection spectrum. The reason for adopting the above-mentioned cleaning order is that following the above order can ensure that the cleaning solution does not adhere to the first and second transparent substrates, and the isopropanol used last can evaporate quickly and completely at room temperature.

[0051] S500, then the first and second light-transmitting substrates are dried with a drying gas; the step of drying the first and second light-transmitting substrates with a drying gas includes: specifically, removing the first and second light-transmitting substrates from the beaker containing isopropanol; drying the first and second light-transmitting substrates with nitrogen gas. Nitrogen gas drying ensures that the isopropanol on the substrate surface evaporates quickly and that nitrogen molecules do not remain on the substrate surface. Compared with letting it evaporate naturally, drying can ensure that dust from the air is not introduced into the substrate surface. Of course, the drying gas can also be an inert gas.

[0052] S600, then, take a sample solution and drop it onto the center of the first surface of the first light-transmitting substrate, and shake the first light-transmitting substrate to make the sample solution spread evenly on the first surface of the first light-transmitting substrate; specifically, use a pipette to take 1 mL of sample solution and drop it onto the center of the first light-transmitting substrate that has been cleaned and dried beforehand. For example, shaking the first light-transmitting substrate to make the sample solution spread evenly on the first surface of the first light-transmitting substrate includes: holding the first light-transmitting substrate with tweezers and tilting the first light-transmitting substrate in various directions so that the sample solution evenly covers the first surface of the first light-transmitting substrate. If the sample itself is powder, the powder can be evenly transferred to the first surface of the first light-transmitting substrate.

[0053] S700: Next, the first transparent substrate is placed on a heating stage with the first surface facing upwards and heated to completely evaporate the solvent in the sample solution on the first surface, leaving the solute portion. In this step, if the solute portion does not completely cover the first transparent substrate and the first surface, the operation of step S600 is repeated until the solute portion completely covers the first surface of the first transparent substrate and reaches a sufficient thickness. In this step, since the spot size in optical testing cannot be infinitely small, the spot size is usually on the order of square millimeters. The sample needs to be uniformly distributed within the spot size (mainly uniform thickness and density) so that the sample within the spot is consistent with the influence of the light beam and has the same properties. In addition, if the uniform area of ​​the sample is small, when the sample is installed on the sample holder, it is usually not possible to completely guarantee that the spot passes exactly through the uniform sample. Therefore, in order to ensure that the obtained data is relatively accurate and has a certain degree of repeatability, it is usually required that the prepared powder has a large area of ​​uniform distribution on the substrate.

[0054] S800, then the second light-transmitting substrate is placed over the solute portion of the first light-transmitting substrate to obtain a sample for spectral measurement; in this embodiment, for example, the second light-transmitting substrate and the first light-transmitting substrate are made of the same material and are the same or slightly larger in size to better cover the sample. Specifically, the operation of placing the second light-transmitting substrate over the solute portion of the first light-transmitting substrate can be to use clean tweezers to pick up the second light-transmitting substrate and gently place it on the first light-transmitting substrate on which the sample is evenly distributed in step S700.

[0055] S900. Finally, the spectral measurement sample is attached to the sample holder using tape around the edge of the second transparent substrate. In this embodiment, the sample holder is generally a disc with a central hole. The first transparent substrate side of the spectral measurement sample is aligned with the hole and pressed tightly against the disc, with the first transparent substrate completely covering the hole. Then, the spectral measurement sample is attached to the disc using tape around the edge of the second transparent substrate, and the sample is sealed within the first and second transparent substrates by the tape.

[0056] In simple terms, the general process of the sample packaging method for spectral measurement of the present invention is as follows:

[0057] 1. Substrate cleaning method:

[0058] (1) After cleaning the substrate with deionized water, immerse it in deionized water and ultrasonically vibrate it for 10 minutes using an ultrasonic cleaner.

[0059] (2) After taking it out, soak it in acetone and use an ultrasonic cleaner to ultrasonically vibrate it for 10 minutes;

[0060] (3) After taking it out, soak it in ethanol and use an ultrasonic cleaner to ultrasonically vibrate it for 10 minutes;

[0061] (4) After taking it out, soak it in isopropanol and ultrasonically clean it for 10 minutes. Then dry the quartz substrate with nitrogen gas for later use.

[0062] 2. Powder sample preparation and packaging technology:

[0063] (1) Use a pipette to draw 1 mL of powder sample solution and drop it onto the center of a pre-cleaned and dried substrate. Hold the substrate with clean tweezers and tilt the substrate in all directions to make the solution evenly cover the substrate surface. The substrate can be heated on a heating stage to make the solvent evaporate completely. If the sample itself is a powder, the powder can be evenly transferred to the substrate surface and then proceed directly to step (3).

[0064] (2) Repeat step (1) until a uniformly distributed powder sample appears on the substrate surface, obtaining a suitable powder thickness. If the powder distribution is not uniform, select a new substrate and repeat steps (1) and (2) until a substrate with a uniformly distributed powder sample is obtained. Since the spot size in optical testing cannot be infinitely small, it is usually on the order of square millimeters. The sample needs to be uniformly distributed within the spot area (mainly uniform thickness and density) so that the sample within the spot is consistent with the influence of the beam and has the same properties. In addition, if the uniform area of ​​the sample is small, when the sample is mounted on the sample holder, it is usually not possible to completely guarantee that the spot passes exactly through the uniform sample. Therefore, in order to ensure that the obtained data is relatively accurate and has a certain degree of repeatability, it is usually required that the prepared powder has a large area of ​​uniform distribution on the substrate.

[0065] (3) Using clean tweezers, pick up another substrate of the same material and specifications and gently place it on the substrate on which the sample is evenly distributed in step (2). Finally, seal the sample on the sample holder with tape, ensuring that each edge of the substrate is securely sealed.

[0066] Test example:

[0067] The sample encapsulation method for spectral measurement of the present invention encapsulates nano-carbon dot powder samples on the sample holder of a terahertz time-domain spectroscopy testing system. In order to eliminate the influence of water vapor and other air molecules in the air on the optical test results, the sample cavity needs to be evacuated. Then, the sample is subjected to terahertz transmission time-domain spectroscopy to verify the spectral response of the encapsulated powder sample to the terahertz time-domain spectral signal in a vacuum environment.

[0068] Figure 2 and Figure 3Photos of the powder sample without complete encapsulation (right) and the encapsulated powder sample (middle) after one vacuum pumping (10-5 mbar) are shown. It can be seen that for the incompletely encapsulated powder carbon dots, even though a substrate layer covers the surface, a large amount of shedding still occurs in the high-vacuum environment. In contrast, the encapsulated powder carbon dots are still evenly distributed on the substrate and can be used for transmission and reflection measurements.

[0069] As Figure 4 shown, the terahertz time-domain spectroscopy signal E2 of the encapsulated powder carbon dot sample ( Figure 4 middle) in a vacuum environment is compared with the signal E1 of the sample without complete encapsulation ( Figure 4 right) and the terahertz transmission signal E0 of the double-layer empty substrate without the powder sample. The magnitudes of their terahertz time-domain signal values from weak to strong are E2 < E1 < E0. This indicates that for the unencapsulated sample, due to the influence of the vacuum environment, the surface powder is prone to shedding, and the powder sample cannot completely cover the substrate, resulting in light leakage in some cavities. Therefore, the obtained terahertz transmission signal is not entirely the signal generated by the interaction between the sample and terahertz light. In contrast, the encapsulated powder sample is well-preserved in a vacuum environment and is still very evenly distributed on the substrate. Therefore, what the terahertz light detects is the terahertz optoelectronic properties of the powder sample itself. This encapsulation method can quickly facilitate the optical detection of powder samples and ensure the accuracy and convenience of optical detection while reducing the encapsulation cost.

[0070] This solution aims to solve the problem of testing powder samples on optical testing instruments without a powder sample holder. The encapsulation in this patent avoids the uneven distribution or even complete loss of powder samples caused by the powder samples being pumped away by the vacuum pump under high vacuum. Compared with testing instruments with a powder sample holder, this method can be applied to most powder samples without damaging the samples. Moreover, the influence of the substrate on the measurement light during the testing process can be eliminated by dividing the measurement results of the two-layer substrate without the sample, thus eliminating the influence of the substrate on the sample.

[0071] Compared with using special or general powder sample holders, the consistency of optical measurement results can be ensured. Compared with pressing the powder into tablets, for materials such as carbon dots and perovskite quantum dots, this solution can prepare samples for optical testing without damaging or causing sample aggregation and property change, and the measurement results of the sample itself can be achieved during the measurement process.

[0072] Compared with directly coating the powder sample on the substrate without covering it with an encapsulating substrate on the upper layer, in a high-vacuum environment, the sample will be lost or completely detached from the substrate, resulting in uneven distribution of the powder sample. This solution solves this problem, making the testing of the sample accurate and repeatable.

[0073] This solution addresses the problem of testing powder samples on optical testing instruments without a powder sample holder. The encapsulation of this invention avoids the uneven distribution or even complete loss of powder samples caused by the vacuum pump removing the powder sample under high vacuum. (1) Compared to testing instruments with a powder sample holder, this method is applicable to most powder samples without damaging the sample, and the influence of the substrate on the measurement light during the test can be eliminated by subtracting the measurement results of the two substrates without the sample; (2) Compared to using special or general powder sample holders, it can ensure the consistency and repeatability of optical measurement results; (3) Compared to pressing powder into sheets, this solution can prepare samples suitable for optical testing without damaging or causing the sample to agglomerate and change its properties, such as carbon dots and perovskite quantum dots, and can achieve the measurement results of the sample itself during the measurement process; (4) Compared to directly coating the powder sample onto the substrate without adding an encapsulation substrate, the sample may be lost or completely detached from the substrate under high vacuum, resulting in uneven distribution of the powder sample. This solution solves this problem, making the test of the sample both accurate and repeatable.

[0074] In summary, the packaging method of this invention is mainly used to solve the problem of powder sample pretreatment for spectroscopic analysis tests such as terahertz spectroscopy, infrared spectroscopy, ultrafast pump-probe, and Raman spectroscopy. These advanced technologies are widely used optical measurement methods. For example, terahertz time-domain spectroscopy (THz-TDS) can be used to study the carrier dynamics of electronic systems, the interactions between small biomolecules in biomolecules, and the low-frequency characteristics of biomacromolecules. Infrared spectroscopy has a wide range of applicability to samples, including solid, liquid, and gaseous samples, and can detect inorganic, organic, and polymeric compounds. Infrared spectroscopy can not only be used to study molecular structure and chemical bonds, such as the determination of force constants and criteria for molecular symmetry, but also as a method for characterizing and identifying chemical species, and has become one of the most commonly used and indispensable tools in modern structural chemistry and analytical chemistry. Spectroscopic analysis techniques such as terahertz spectroscopy and infrared spectroscopy have been widely used in materials science, life sciences, physics, chemistry, and other fields. However, pretreatment of the powder sample before testing is required before loading it onto a sample holder for testing. This invention provides a rapid, reliable, and low-cost method for packaging powder samples, enabling the samples to be tested to undergo spectral analysis and testing, such as terahertz spectroscopy and infrared spectroscopy, in a high vacuum environment after pretreatment.

[0075] The above-described embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for packaging a sample for spectral measurement, characterized in that, The encapsulation method includes: Provide a first transparent substrate and a second transparent substrate; The first and second light-transmitting substrates were cleaned with deionized water. Next, deionized water was used as the cleaning agent and an ultrasonic cleaner was used to ultrasonically clean the first and second transparent substrates. Next, an organic solvent was used as a cleaning agent and an ultrasonic cleaner was used to ultrasonically clean the first and second light-transmitting substrates. Then, the first and second light-transmitting substrates were dried with a drying gas. Next, a sample solution is dropped onto the center of the first surface of the first light-transmitting substrate, and the first light-transmitting substrate is shaken to make the sample solution spread evenly on the first surface of the first light-transmitting substrate; The first transparent substrate is then placed on a heating stage with the first surface facing upwards and heated to completely evaporate the solvent in the sample solution on the first surface, leaving the solute portion. Then, the second light-transmitting substrate is placed over the solute portion of the first light-transmitting substrate to obtain a sample for spectral measurement.

2. The method for packaging a sample for spectral measurement according to claim 1, characterized in that, The method of cleaning the first and second light-transmitting substrates with deionized water can be rinsing or brushing.

3. The method for packaging a sample for spectral measurement according to claim 2, characterized in that, The next step involves using deionized water as a cleaning agent and employing an ultrasonic cleaner to perform ultrasonic cleaning on the first and second light-transmitting substrates, including: The first and second transparent substrates, after being cleaned with deionized water, are placed in a beaker containing deionized water. The beaker containing deionized water is then placed in the cleaning tank of an ultrasonic cleaner and subjected to ultrasonic vibration for 10-20 minutes.

4. The method for packaging a sample for spectral measurement according to claim 3, characterized in that, The next step involves using an organic solvent as a cleaning agent and an ultrasonic cleaner to perform ultrasonic cleaning on the first and second light-transmitting substrates, including: First, remove the first and second transparent substrates from the cleaning tank of the ultrasonic cleaner containing deionized water; place the first and second transparent substrates into a beaker containing acetone, and then place the beaker containing acetone into the cleaning tank of the ultrasonic cleaner and ultrasonically vibrate for 10-20 minutes. Next, the first and second transparent substrates are removed from the cleaning tank of the ultrasonic cleaner containing acetone; then the first and second transparent substrates are placed in a beaker containing ethanol, and then the beaker containing ethanol is placed in the cleaning tank of the ultrasonic cleaner and ultrasonically vibrated for 10-20 minutes. Then, the first and second transparent substrates are removed from the cleaning tank of the ultrasonic cleaner containing ethanol; the first and second transparent substrates are then placed into a beaker containing isopropanol, and the beaker containing isopropanol is then placed into the cleaning tank of the ultrasonic cleaner and ultrasonically vibrated for 10-20 minutes.

5. The method for packaging a sample for spectral measurement according to claim 4, characterized in that, The subsequent drying of the first and second light-transmitting substrates with a drying gas includes: Remove the first and second transparent substrates from the beaker containing isopropanol; The first and second light-transmitting substrates were dried with nitrogen gas.

6. The method for packaging a sample for spectral measurement according to claim 1, characterized in that, The step of shaking the first light-transmitting substrate to spread the sample solution evenly on the first surface of the first light-transmitting substrate includes: Hold the first light-transmitting substrate with tweezers and tilt the first light-transmitting substrate in various directions so that the sample solution evenly covers the first surface of the first light-transmitting substrate.

7. The method for packaging a sample for spectral measurement according to claim 6, characterized in that, The encapsulation method further includes: The spectral measurement sample is attached to the sample holder by using tape around the edge of the second transparent substrate.

8. The method for packaging a sample for spectral measurement according to any one of claims 1-7, characterized in that, The first and second light-transmitting substrates are quartz substrates, sapphire substrates, silicon oxide substrates, or organic thin film substrates.

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