Multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring and its usage method

Abstract

The present invention provides a multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring and a usage method thereof; an X-axis linear motion unit disposed on an experimental platform, a pipette pump fixed on the X-axis linear motion unit, and two groups of pipette needle assemblies communicated through the pipette pump; a liquid level monitoring vision camera assembly driven by a Z-axis linear motion unit is simultaneously fixed on the X-axis linear motion unit; a pipette needle cleaning unit and two rows of extraction bottle racks are provided on the experimental platform, and each row of bottle racks can place multiple groups of extraction bottles; the liquid level monitoring vision camera assembly is used to detect whether a liquid-liquid extraction liquid level is generated and monitor the liquid level; the cleaning unit is used to clean the pipette needle body; a barcode reader is further provided on the experimental platform. The present invention realizes the pretreatment and liquid-liquid extraction tasks of multiple samples through automatic control of multiple stations, avoids reagent contamination by cleaning the pipette needle body through the cleaning unit, realizes automatic switching of multiple tasks, improves efficiency, and reduces mistakes.

Description

Technical Field

[0001] The present invention belongs to the field of laboratory automated extraction equipment, and particularly relates to a multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring and a usage method thereof. Background Art

[0002] Liquid-liquid extraction is based on the different distribution coefficients of solutes in two immiscible liquids to achieve the separation and concentration of target substances. The phase to be separated is the extraction phase, and the solvent after extraction is called the raffinate phase. Liquid-liquid extraction is one of the common experimental and analytical sample pretreatment methods. The extraction test operation is complicated, has strong repeatability, long duration, and has continuity requirements. At present, the vast majority of these series of complex test operations are completed manually, which causes great harm to the human body, has low efficiency, and the error operation rate also increases during long-term tests. Summary of the Invention

[0003] In view of this, the present invention aims to provide a multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring and a usage method thereof, which realizes the pretreatment of multiple samples through automated control of multiple stations, improves efficiency, and reduces mistakes.

[0004] To achieve the above object, the technical solution of the present invention is realized as follows:

[0005] A multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring includes an X-axis linear motion unit arranged on an experimental platform, a liquid transfer pump, a liquid level monitoring vision camera assembly, two groups of liquid transfer needle assemblies, an extraction bottle rack, a cleaning unit, and a computer central control system;

[0006] The two groups of extraction bottle racks are arranged in parallel on the experimental platform, and a magnetic stirrer is provided at the bottom of each group of extraction bottle racks. The extraction bottle rack is used to place extraction bottles, and the magnetic stirrer is used to rotate the liquid in the extraction bottle to achieve full mixing of the liquid;

[0007] The X-axis linear motion unit is arranged between the two groups of extraction bottle racks, and the X-axis linear motion unit is further provided with a Z-axis linear motion unit to realize the movement of the Z-axis linear motion unit in the X-axis direction;

[0008] The Z-axis linear motion unit is provided with a liquid transfer pump and two groups of liquid transfer needle assemblies, and each group of liquid transfer needle assemblies corresponds to a Z-axis linear motion unit; realizing the simultaneous movement or single movement of the two groups of liquid transfer needle assemblies in the Z-axis direction; the two groups of liquid transfer needle assemblies are interconnected and are respectively connected to the liquid transfer pump; the two groups of liquid transfer needle assemblies correspond one by one to the two groups of extraction bottle racks;

[0009] A cleaning unit is provided at the end of each group of extraction bottle racks, and the cleaning unit is used to clean the inner and outer walls of the liquid transfer needles of the liquid transfer needle assemblies;

[0010] The X-axis linear motion unit is also provided with a liquid level monitoring vision camera assembly for monitoring whether a liquid-liquid extraction liquid level is generated in the extraction bottle;

[0011] A barcode reader is also provided on the experimental platform; the X-axis linear motion unit, the Z-axis linear motion unit, the pipette pump, the liquid level monitoring vision camera assembly, the magnetic stirrer and the cleaning unit are all connected to the computer central control system.

[0012] Further, the position where each magnetic stirrer is located is used as a work station, and the position information of each work station is stored in the computer central control system.

[0013] Further, the moving trajectories of the two pipette needle bodies of the two pipette needle assemblies respectively correspond to two groups of extraction bottle racks.

[0014] Further, the pipette needle body is used to suck the liquid in the extraction bottle or input the liquid into the extraction bottle.

[0015] Further, the cleaning unit includes a cleaning pipe and a drainage pipeline. The cleaning pipe is arranged inside the drainage pipeline. The bottom of the cleaning pipe is connected to a cleaning liquid storage container through a first pipeline, and the bottom of the drainage pipeline is connected to a waste liquid drainage system through a second pipeline.

[0016] Further, the diameter of the drainage pipeline is larger than that of the cleaning pipe, and the diameter of the cleaning pipe is larger than that of the pipette needle body.

[0017] Further, a cleaning pump and an electromagnetic valve are provided in the first pipeline, and the cleaning pump and the electromagnetic valve are signal-connected to the computer central control system.

[0018] A method for using a multi-station continuous full-automatic liquid-liquid extraction device based on AI vision liquid level monitoring is characterized by comprising the following steps:

[0019] The extraction liquid and the mixed liquid are in the same extraction bottle;

[0020] S11. Determine the components; place the extraction bottle containing the liquid to be extracted into the extraction bottle rack in the row with the magnetic stirrer, and place a new empty bottle of the same specification at the corresponding position in the other row of extraction bottle racks; before placing the extraction bottle into the extraction bottle rack, the bottle must be held to the barcode reader to read the identity code of the bottle;

[0021] S12. Magnetic stirring; control the magnetic stirrer to work, set the rotation speed and stirring time of the magnetic stirrer through the computer central control system, and after stirring is completed, the computer central control system controls the magnetic stirrer to turn off.

[0022] S13. Layering; let it stand for layering and wait for extraction.

[0023] S14. Analyze the liquid level: The extraction bottle that has completed static settling sends a notification instruction to the computer central control system. The computer central control system controls the vision camera and the pipetting unit to move to this position through the X-axis linear movement unit. Driven by the X-axis linear movement unit, the vision camera scans and takes pictures of the extraction bottle after static settling from top to bottom, and determines the position of the liquid separation level by an image algorithm.

[0024] S15. Extract and pipette: The controller of the Z-axis linear movement unit automatically moves the first pipetting needle downward. According to the actual situation of the liquid to be extracted in the upper or lower layer after stratification, the first pipetting needle moves down to the top of the liquid separation level or the bottom of the bottle, and the second pipetting needle simultaneously moves to an empty bottle on the other side. Control the pipetting pump to work and perform a pipetting operation to move the liquid in the extraction bottle to the empty bottle. The position of the second pipetting needle in the empty bottle is set by the computer central control system. During pipetting, the liquid level in the second extraction bottle is detected by the liquid level monitoring vision camera assembly. When the liquid level reaches the tip position of the second pipetting needle, stop pipetting.

[0025] S16. Clean the pipetting needles: The remaining substances in the extraction bottle are extraction residues. At this time, the first pipetting needle and the second pipetting needle that have completed the pipetting operation enter the cleaning unit for cleaning under the cooperation of the X-axis linear movement unit and the Z-axis linear movement unit, and wait for the next extraction after cleaning.

[0026] S17. Collect the extraction bottle: Use the manipulator to take away the second extraction bottle after extraction is completed; continue the above cyclic operation until all extractions are completed.

[0027] The extract and the mixture are in two extraction bottles respectively.

[0028] S21. Place in separate bottles: Place the extract in the first extraction bottle and the mixture in the second extraction bottle.

[0029] S22. Pipette: The computer central control system controls the second moving frame to move the first pipetting needle into the first extraction bottle and the second pipetting needle into the second extraction bottle, and controls the pipetting pump to work to add the extract in the first extraction bottle to the mixture in the second extraction bottle.

[0030] S23. Stratify: After adding, the first pipetting needle and the second pipetting needle are lifted, and the computer central control system controls the magnetic stirrer to work to shake the second extraction bottle to accelerate stratification and set the shaking time.

[0031] S24. Liquid level detection: The first moving frame controls the liquid level monitoring vision camera assembly to move to the position of the second extraction bottle to detect the stratification situation of the liquid level. If the detected stratification is qualified, the extraction is completed. If the detected stratification is unqualified, continue the above operation until the extraction is completed.

[0032] S25. Pipette needle cleaning: After the extraction is completed, the pipette needle is driven by the second moving rack and enters the cleaning unit for cleaning. After cleaning, it waits for the next extraction. The manipulator takes away the second extraction bottle after the extraction is completed.

[0033] Compared with the prior art, the multi-station continuous fully automatic liquid-liquid extraction device and its usage method based on AI vision liquid level monitoring of the present invention have the following advantages:

[0034] (1) The multi-station continuous fully automatic liquid-liquid extraction device and its usage method based on AI vision liquid level monitoring of the present invention are controlled by a computer central control system, which issues task instructions simultaneously, pre-treats multiple samples on multiple stations at the same time, works automatically in segments, and automatically detects the work progress.

[0035] (2) The multi-station continuous fully automatic liquid-liquid extraction device and its usage method based on AI vision liquid level monitoring of the present invention adopt an automatically controlled moving rack, and the vision liquid level detector monitors in real time. After the extraction bottle is completed, the extraction bottle is automatically transported by the manipulator, saving manpower and making the extraction quality higher.

[0036] (3) The multi-station continuous fully automatic liquid-liquid extraction device and its usage method based on AI vision liquid level monitoring of the present invention use a liquid transfer pump for automatic liquid transfer, automatic needle washing and pipeline cleaning, realizing a series of complex test processes of multi-task continuous fully automatic liquid-liquid extraction; it can fully realize an intelligent fully automatic unmanned experimental process, with higher efficiency and lower misoperation rate compared with manual extraction, enabling the system to continuously execute multiple extraction tasks without pollution and realizing liquid-liquid extraction efficiently and automatically. Description of the Drawings

[0037] The drawings forming a part of the present invention are used to provide a further understanding of the present invention. The schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation to the present invention. In the drawings:

[0038] Figure 1 It is a schematic diagram of the multi-station continuous fully automatic liquid-liquid extraction device and its usage method based on AI vision liquid level monitoring in the embodiment of the present invention.

[0039] Description of the reference numerals:

[0040] 1. Experimental platform; 2. X-axis linear movement unit; 3. Extraction bottle; 4. Liquid transfer pump; 5. Liquid level monitoring vision camera assembly; 6. Pipette needle assembly; 7. Extraction bottle rack; 8. Cleaning unit; 81. Cleaning pipe; 82. Drainage pipeline; 9. Computer central control system. Detailed Embodiments

[0041] It should be noted that, without conflict, the embodiments in the present invention and the features in the embodiments can be combined with each other.

[0042] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first", "second", etc. may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise specified, the meaning of "a plurality" is two or more.

[0043] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood through specific situations.

[0044] The present invention will be described in detail below with reference to the drawings and in conjunction with embodiments.

[0045] A multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring, as Figure 1 shown, includes an X-axis linear motion unit arranged on the experimental platform 1, a liquid transfer pump 4, a liquid level monitoring vision camera assembly 5, two groups of liquid transfer needle assemblies 6, an extraction bottle 3 rack, a cleaning unit 8, and a computer central control system 9;

[0046] The two groups of the extraction bottle 3 racks are arranged in parallel on the experimental platform 1. A magnetic stirrer is provided at the bottom of each group of the extraction bottle 3 racks. The extraction bottle 3 racks place the extraction bottles 3, and the magnetic stirrer is used to rotate the liquid in the extraction bottles 3 to achieve full mixing of the liquid;

[0047] Example 1, magnetic stirrers are provided at the bottoms of both groups of extraction bottle racks. Example 2, magnetic stirrers are provided at the bottoms of only one group of the two groups of extraction bottle racks;

[0048] The X-axis linear motion unit is arranged between two sets of extraction bottle racks 3. The X-axis linear motion unit is also provided with a Z-axis linear motion unit to realize the movement of the Z-axis linear motion unit in the X-axis direction. The X-axis linear motion unit and the Z-axis linear motion unit are respectively modules capable of realizing linear movement, which are components that can be purchased on the market;

[0049] The Z-axis linear motion unit is provided with a liquid transfer pump 4 and two sets of liquid transfer needle assemblies 6. Each set of liquid transfer needle assemblies 6 corresponds to one Z-axis linear motion unit; it realizes the simultaneous movement or single movement of the two sets of liquid transfer needle assemblies 6 in the Z-axis direction; the two sets of liquid transfer needle assemblies 6 are interconnected, and liquid exchange is carried out between them through a transfer pump, and they are respectively connected to the liquid source through the liquid transfer pump 4 for sucking the extraction liquid; the two sets of liquid transfer needle assemblies 6 correspond one by one to the two sets of extraction bottle racks 3;

[0050] Each end of each set of extraction bottle racks 3 is provided with a cleaning unit 8, and the cleaning unit 8 is used to clean the inner and outer walls of the liquid transfer needle body of the liquid transfer needle assembly 6;

[0051] The X-axis linear motion unit is also provided with a liquid level monitoring vision camera assembly 5 for monitoring whether a liquid-liquid extraction liquid level is generated in the extraction bottle 3;

[0052] A barcode reader is also provided on the experimental platform 1; the X-axis linear motion unit, the Z-axis linear motion unit, the liquid transfer pump 4, the liquid level monitoring vision camera assembly 5, the magnetic stirrer and the cleaning unit 8 are all connected to the computer central control system 9.

[0053] Preferably, the position of each magnetic stirrer is used as a work station, and the position information of each work station is stored in the computer central control system 9.

[0054] As Example 1, the layout of the magnetic stirrers is chaotic and disorderly. Before use, the position information of these magnetic stirrers is input into the computer central control system 9, and the liquid transfer needle body is controlled to the target set position through the computer central control system 9;

[0055] As Example 2, the magnetic stirrers in the extraction area are grouped and distributed. At least every two magnetic stirrers are grouped as an extraction group, and the extraction bottles 3 and the magnetic stirrers correspond one by one. For example: two magnetic stirrers are grouped as one group, and there are a total of 4 groups arranged in an orderly manner, that is, the magnetic stirrers are arranged in two rows, with 4 in each row; specifically, the magnetic stirrers are devices that can be purchased on the market, and the liquid in the extraction bottle 3 is shaken by the magnetic stirrers to accelerate stratification.

[0056] Preferably, the moving trajectories of the two liquid transfer needle bodies of the two liquid transfer needle assemblies 6 respectively correspond to the two sets of extraction bottle racks 3.

[0057] As an embodiment, the number of pipette needle bodies is two or more. Two or more pipette needle bodies are connected to the pipette pump 4 through pipelines. The pipette pump 4 is further connected to other liquid sources through pipelines. For two or more pipette needle bodies, one pipette needle body may correspond to one liquid source, or multiple pipette needle bodies may correspond to one liquid source. Preferably, two or more pipette needle bodies can be connected through the pipette pump 4. That is, for pipette needle body A and pipette needle body B, under the operation of the pipette pump 4, pipette needle body A can suck liquid C, and then output liquid C to the target position through pipette needle body B;

[0058] The pipette pump 4 is a product that can be purchased in the market. Preferably, the liquid can flow directly in the pipeline without passing through the pipette pump 4 to avoid contamination of the liquid after passing through the pipette pump 4 and inaccurate experimental data.

[0059] Preferably, the pipette needle body is used to suck the liquid in the extraction bottle 3 or input the liquid into the extraction bottle 3.

[0060] Preferably, the cleaning unit 8 includes a cleaning pipe 81 and a drain pipeline 82. The cleaning pipe 81 is arranged inside the drain pipeline 82. The bottom of the cleaning pipe 81 is connected to the cleaning liquid storage container through a first pipeline, and the bottom of the drain pipeline 82 is connected to the waste liquid drainage system through a second pipeline. Preferably, a cleaning pump and an electromagnetic valve are provided in the first pipeline, and the cleaning pump and the electromagnetic valve are signal-connected to the computer central control system 9.

[0061] The computer central control system 9 controls the cleaning pump and the electromagnetic valve to open. Clean liquid flows from the liquid source through the first pipeline to the cleaning pipe 81. The water for cleaning flows out unidirectionally and continuously. The water flows out of the cleaning pipe 81 and into the drain pipeline 82, and then is discharged, without causing pollution and damage to the environment of the workbench.

[0062] Preferably, the diameter of the drain pipeline 82 is larger than the diameter of the cleaning pipe 81, and the diameter of the cleaning pipe 81 is larger than the diameter of the pipette needle body. The positions of the drain pipeline 82 and the cleaning pipe 81 are fixed, and the position information is input into the computer central control system 9. Under the control of the computer central control system 9, it is convenient for the pipette needle body to quickly and accurately enter the cleaning pipe 81 under the drive of the second moving stage.

[0063] Preferably, the computer central control system 9 is a PLC or other electronic control unit. By using a mature computer central control system 9 to control the overall extraction process, it reduces physical objects and improves efficiency.

[0064] Before each extraction process starts, the manipulator grabs the extraction bottle 3 and moves it to the barcode reader device. An identity information QR code is provided on the body of each extraction bottle 3. After the barcode reader reads its identity, it transmits a signal to the computer central control system 9. After the computer central control system 9 identifies it, the extraction bottle 3 is placed at the target position. After this procedure, the correspondence between the identity number of the extraction bottle 36 and the liquid data is uploaded to the computer central control system 9 to complete the recording and tracking of this data identity.

[0065] The usage method of a multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring includes the following steps:

[0066] Example 1: The extraction liquid and the mixed liquid are in the same extraction bottle 3.

[0067] S11. Determine the components: Place the extraction bottle 3 containing the liquid to be extracted into the extraction bottle rack in the row with the magnetic stirrer, and place a new empty bottle of the same specification at the corresponding position in the other row of the extraction bottle rack. For the fully automatic liquid-liquid extraction system, the manipulator accepts the central control instruction to put in and take out various bottles; for the independent liquid-liquid extraction station, the personnel complete the putting in and taking out of various bottles. Whether it is a fully automatic system or an independent workstation, before placing the extraction bottle 3 into the extraction bottle rack before putting various bottles into the liquid-liquid extraction station, the bottle must be taken to the barcode reader to read the identity code of the bottle; the identity code on the bottle can be a barcode, QR code, RFID code, etc.

[0068] S12. Magnetic stirring: Control the magnetic stirrer to work, set the rotation speed and stirring time of the magnetic stirrer through the computer central control system 9, and after stirring is completed, the computer central control system 9 controls the magnetic stirrer to turn off.

[0069] S13. Layers separation: Let it stand for layers separation and wait for extraction.

[0070] S14. Analyze the liquid level: The extraction bottle 3 that has completed standing sends a notification instruction to the computer central control system 9. The computer central control system 9 controls the vision camera and the pipetting unit to move to this position through the X-axis linear movement unit 2. Driven by the X-axis linear movement unit 2, the vision camera scans and shoots the extraction bottle 3 after standing from top to bottom, and determines the position of the liquid separation surface by the image algorithm.

[0071] S15. Extraction and pipetting; the controller of the Z-axis linear movement unit moves the first pipetting needle downward automatically; according to the actual situation of whether the liquid to be extracted is in the upper or lower layer after layering, the first pipetting needle moves down to the top of the liquid separation surface or the bottom of the bottle, and the second pipetting needle moves to an empty bottle on the other side at the same time. Control the pipetting pump 4 to work, and perform pipetting operation to move the liquid in the extraction bottle 3 to the empty bottle. The position of the second pipetting needle in the empty bottle is set by the computer central control system 9. During pipetting, the liquid level of the second extraction bottle 3 is detected by the liquid level monitoring vision camera assembly. When the liquid level reaches the tip position of the second pipetting needle, stop pipetting;

[0072] S16. Pipetting needle cleaning; the remaining substances after extraction are in the extraction bottle 3. At this time, the first pipetting needle and the second pipetting needle that have completed the pipetting operation enter the cleaning unit 8 for cleaning under the cooperation of the X-axis linear movement unit 2 and the Z-axis linear movement unit, and wait for the next extraction after cleaning;

[0073] S17. Collection of the extraction bottle 3; use the manipulator to take away the second extraction bottle 3 after extraction; continue the above cyclic operation until all extractions are completed.

[0074] Example 2. The extraction liquid and the mixed liquid are in two extraction bottles 3 respectively;

[0075] S21; Placing in separate bottles; the extraction liquid is placed in the first extraction bottle 3, and the mixed liquid is placed in the second extraction bottle 3.

[0076] S22; Pipetting; the computer central control system 9 controls the second moving frame to move the first pipetting needle into the first extraction bottle 3 and the second pipetting needle into the second extraction bottle 3, and controls the pipetting pump 4 to work to add the extraction liquid in the first extraction bottle 3 into the mixed liquid in the second extraction bottle 3.

[0077] S23. Layering; after adding, the first pipetting needle and the second pipetting needle are lifted, and the computer central control system 9 controls the magnetic stirrer to work to shake the second extraction bottle 3 to accelerate layering and set the shaking time;

[0078] S24. Liquid level detection; the first moving frame controls the liquid level monitoring vision camera assembly to move to the position of the second extraction bottle 3 to detect the layering situation of the liquid level. If the detected layering is qualified, the extraction is completed. If the detected layering is unqualified, continue the above operation until the extraction is completed;

[0079] S25. Pipetting needle cleaning; the pipetting needle after extraction enters the cleaning unit 8 for cleaning under the drive of the second moving frame, and waits for the next extraction after cleaning; use the manipulator to take away the second extraction bottle 3 after extraction.

[0080] Example 3. Through the cooperation among the cleaning unit 8, the pipetting needle body, and the pipetting pump 4, the cleaning of the pipetting needle body and the pipeline is achieved, enabling the device to continuously perform the extraction task without pollution.

[0081] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. Method for using a multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring, characterized in that The extraction device includes an X-axis linear motion unit arranged on the experimental platform, a pipette pump, a liquid level monitoring vision camera assembly, two groups of pipette needle assemblies, an extraction bottle rack, a cleaning unit and a computer central control system; The two groups of extraction bottle racks are arranged in parallel on the experimental platform. A magnetic stirrer is provided at the bottom of each group of extraction bottle racks. The extraction bottle rack is used to place extraction bottles, and the magnetic stirrer is used to rotate the liquid in the extraction bottle to achieve full mixing of the liquid; The X-axis linear motion unit is arranged between the two groups of extraction bottle racks. The X-axis linear motion unit is also provided with a Z-axis linear motion unit to realize the movement of the Z-axis linear motion unit in the X-axis direction; The Z-axis linear motion unit is provided with a pipette pump and two groups of pipette needle assemblies. Each group of pipette needle assemblies corresponds to a Z-axis linear motion unit; to realize the simultaneous movement or single movement of the two groups of pipette needle assemblies in the Z-axis direction; the two groups of pipette needle assemblies are connected to each other and are respectively connected to the pipette pump; the two groups of pipette needle assemblies correspond one by one to the two groups of extraction bottle racks; A cleaning unit is provided at the end of each group of extraction bottle racks. The cleaning unit is used to clean the inner and outer walls of the pipette needle body of the pipette needle assembly; The X-axis linear motion unit is also provided with a liquid level monitoring vision camera assembly for monitoring whether a liquid-liquid extraction separation liquid level is generated in the extraction bottle; A barcode reader is also provided on the experimental platform; the X-axis linear motion unit, the Z-axis linear motion unit, the pipette pump, the liquid level monitoring vision camera assembly, the magnetic stirrer and the cleaning unit are all connected to the computer central control system; The method of use includes the following steps: The extraction liquid and the mixed liquid are in the same extraction bottle; S11. Determine the composition; place the extraction bottle containing the liquid to be extracted into the extraction bottle rack in the row with the magnetic stirrer, and place a new empty bottle of the same specification at the corresponding position in the other row of extraction bottle racks; before placing the extraction bottle into the extraction bottle rack, the bottle must be held to the barcode reader to read the identity code of the bottle; S12. Magnetic stirring; control the magnetic stirrer to work, set the rotation speed and stirring time of the magnetic stirrer through the computer central control system, and after stirring, the computer central control system controls the magnetic stirrer to turn off; S13. Layering; let it stand for layering and wait for extraction; S14. Analyze the liquid level; the extraction bottle that has completed standing sends a notification command to the computer central control system. The computer central control system controls the vision camera and the pipetting unit to move to this position through the X-axis linear movement unit. The vision camera scans and photographs the standing extraction bottle from top to bottom under the drive of the X-axis linear movement unit, and determines the position of the liquid separation liquid level by an image algorithm; S15. Extraction and pipetting; the controller of the Z-axis linear movement unit moves the first pipetting needle downward automatically; according to the actual situation of the liquid to be extracted in the upper or lower layer of the stratified liquid, the first pipetting needle moves down to the top of the liquid separation surface or the bottom of the bottle, and the second pipetting needle moves to an empty bottle on the other side at the same time. Control the pipetting pump to work to perform the pipetting operation to move the liquid in the extraction bottle to the empty bottle. The position of the second pipetting needle in the empty bottle is set by the computer central control system. During pipetting, the liquid level of the second extraction bottle is detected by the liquid level monitoring vision camera assembly. When the liquid level reaches the tip position of the second pipetting needle, stop pipetting; S16. Pipetting needle cleaning; the remaining extraction in the extraction bottle. At this time, the first pipetting needle and the second pipetting needle that have completed the pipetting operation enter the cleaning unit for cleaning under the cooperation of the X-axis linear movement unit and the Z-axis linear movement unit, and wait for the next extraction after cleaning; S17. Extraction bottle collection; use the manipulator to take away the second extraction bottle after extraction is completed; continue the above cyclic operation until all extractions are completed; The extraction liquid and the mixed liquid are in two extraction bottles respectively; S21. Placing in separate bottles; place the extraction liquid in the first extraction bottle and the mixed liquid in the second extraction bottle, S22. Pipetting; the computer central control system controls the second moving rack to move the first pipetting needle into the first extraction bottle and the second pipetting needle into the second extraction bottle, and controls the pipetting pump to work to add the extraction liquid in the first extraction bottle into the mixed liquid in the second extraction bottle, S23. Stratification; after adding, the first pipetting needle and the second pipetting needle are lifted, and the computer central control system controls the magnetic stirrer to work to shake the second extraction bottle to accelerate stratification, and set the shaking time; S24. Liquid level detection; the first moving rack controls the liquid level monitoring vision camera assembly to move to the position of the second extraction bottle to detect the stratification situation of the liquid level. If the detected stratification is qualified, the extraction is completed. If the detected stratification is unqualified, continue the above operations until the extraction is completed; S25. Pipetting needle cleaning; the pipetting needle after extraction is completed enters the cleaning unit for cleaning under the drive of the second moving rack, and waits for the next extraction after cleaning; use the manipulator to take away the second extraction bottle after extraction is completed.

2. The usage method of the multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring according to claim 1, characterized in that: The position where each magnetic stirrer is located is used as a work station, and the position information of each work station is stored in the computer central control system.

3. The usage method of the multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring according to claim 1, characterized in that: The moving trajectories of the two pipetting needle bodies of the two pipetting needle assemblies respectively correspond to two groups of extraction bottle racks.

4. The method of using the multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring according to claim 2, characterized in that: The pipetting needle body is used to suck the liquid in the extraction bottle or input the liquid into the extraction bottle.

5. The usage method of the multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring according to claim 1, characterized in that: The cleaning unit includes a cleaning pipe and a drain pipeline. The cleaning pipe is arranged in the drain pipeline. The bottom of the cleaning pipe is connected to the cleaning liquid storage container through the first pipeline, and the bottom of the drain pipeline is communicated with the waste liquid drainage system through the second pipeline.

6. The method for using a multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring according to claim 5, characterized in that: The diameter of the drain pipeline is larger than the diameter of the cleaning pipe, and the diameter of the cleaning pipe is larger than the diameter of the pipetting needle body.

7. The method of using the multi-station continuous fully automatic liquid-liquid extraction device based on AI vision liquid level monitoring according to claim 5, characterized in that: A cleaning pump and an electromagnetic valve are provided in the first pipeline, and the cleaning pump and the electromagnetic valve are signal-connected to the computer central control system.

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