A chemiluminescence detection device and its detection method

Abstract

The present application discloses a chemiluminescence detection device, comprising: a housing, a mounting rack disposed inside the housing, a sample inlet chamber door and a sample outlet chamber door disposed on one side of the housing, a human-computer interaction window embedded in the front of the housing, an injection mechanism, a sample processing mechanism, a detection module and a sample outlet mechanism are sequentially annularly disposed on the mounting rack corresponding to a turntable mechanism, and a main control module is disposed on the side of the mounting rack and is electrically connected to the detection module and each mechanism. The device uses a light excitation method to provide energy for the corresponding sample, causing a series of reactions in the sample in the reagent, and after performing specific conversions on the measured light intensity readings, the detection of the sample is achieved. At the same time, this device provides 16 detection channels. After the sample is added, the sample is automatically injected, and the sample is automatically processed, mixed, incubated and detected, without the need for professional personnel to be on duty, simplifying the operation and the working hours of personnel.

Description

Technical Field

[0001] This application relates to the field of chemiluminescence detection technology, and particularly relates to a chemiluminescence detection device and a detection method thereof. Background Art

[0002] Chemiluminescence detection equipment is mainly used for the detection of related reagents based on the chemiluminescence method excited by light. Currently, common semi-automatic chemiluminescence equipment is based on equipment using the enzyme-catalyzed reaction luminescence or electrochemiluminescence method, and only provides a single-channel test or requires manual sample introduction or manual reagent strip removal. This greatly limits the throughput of the equipment and the working hours of operators.

[0003] CN112730389A discloses a chemiluminescence detection device, including a liquid transfer robotic arm mechanism, a card feeding mechanism, an incubation module, a cleaning module, a transportation module, a reading module and a housing. The bottom end of the housing is bolted with a tabletop. The outer bottom of the housing is hinged with a lower machine cover. A touch screen is embedded above the lower machine cover. A sample rack and a tip box are fixedly connected to the upper surface of the tabletop. The liquid transfer robotic arm mechanism includes a motion guide rail and a sampling head. The bottom end of the sampling head is an electric telescopic structure. The present invention uses an incubation module, a transportation module, a cleaning module and a reading module, and cooperates with the guide rails connected to each component to realize the incubation, transportation, cleaning and final reading procedures of the sample. The whole process is mechanized control, making the whole test process coherent and fast, and the cooperation between mechanisms is tight, which is superior to traditional chemiluminescence detection equipment.

[0004] CN110940818A discloses a highly integrated fully automatic chemiluminescence detection device. Compared with traditional large-scale chemiluminescence detection equipment, the chemiluminescence detection device of the present invention simultaneously includes a control module, a first motor, a reagent wheel disc compartment, a sampling module, a cleaning module, a second motor and a detection module. It has the advantage of high integration, greatly reducing the overall volume and weight of the chemiluminescence detection equipment.

[0005] The above discloses the detection of liquid samples, which requires reagent preparation and complex operations. Currently, in order to improve the detection speed, reagent strips are selected for sampling and detection. Reagent strips can be prepared in advance and have a long shelf life, and can be mass-produced to meet the situation of a particularly large number of detections in a short time. Especially for the detection of novel coronavirus infection, the existing detection methods are difficult to meet the large-scale detection requirements, and there is no corresponding fast, accurate and intelligent equipment to complete the detection. The detection efficiency is low, and the requirements for detection personnel are high, and the degree of automation is low. Summary of the Invention

[0006] In view of the above problems, the present application provides a chemiluminescence detection device and a detection method thereof, which can complete the process of sample processing, detection and exit without human intervention after the reagent strip sample is placed in the injection slot.

[0007] In the first aspect, the present application provides a chemiluminescence detection device, comprising: a casing, and a mounting frame arranged in the casing, and a sample injection chamber door and a sample return chamber door arranged on one side of the casing, a human-computer interaction window embedded in the front of the casing, and a sample injection mechanism, a turntable mechanism, a sample return mechanism, a main control module, a sample processing mechanism and a detection module arranged on the mounting frame, the sample injection mechanism is connected to the sample injection chamber door and the turntable mechanism, the sample return mechanism is connected to the turntable mechanism and the sample return chamber door, the turntable mechanism is arranged in the mounting frame, the sample injection mechanism, the sample processing mechanism, the detection module and the sample return mechanism are arranged in a ring on the mounting frame in sequence corresponding to the turntable mechanism, and the main control module is arranged on the side of the mounting frame and is connected to the detection module and each mechanism by electrical signals.

[0008] In some embodiments, the injection mechanism is provided with a two-stage propulsion mechanism, the first-stage propulsion mechanism and the second-stage propulsion mechanism are connected by a push-card assembly, the first-stage propulsion mechanism is arranged in the installation, the second-stage propulsion mechanism is arranged on the installation frame, and the first-stage propulsion mechanism and the second-stage propulsion mechanism are provided with a driving assembly.

[0009] The driving assembly is provided with a base plate, a sample feeding motor, a synchronous wheel, a driven wheel, a linear guide rail, a connecting plate, a synchronous belt, a pressure plate and a photoelectric switch. A vertical mounting plate is provided on one side of the base plate. The sample feeding motor is fixed on the mounting plate. The synchronous wheel is fastened to the rotating shaft of the sample feeding motor. The driven wheel is arranged on the other end of the base plate. The linear guide rail is arranged on the base plate. The connecting plate is arranged on the slider of the linear guide rail. The synchronous belt is arranged on the synchronous wheel and the driven wheel and is fixed to the connecting plate through the pressure plate. The photoelectric switches are respectively arranged at the front end of the primary propulsion mechanism and the secondary propulsion mechanism. The end of the mechanism; the connecting plate of the first-stage propulsion mechanism is matched with the photoelectric switch for induction, and the front end of the connecting plate of the first-stage propulsion mechanism is also provided with a sample injection slot, and the connecting plate of the second-stage propulsion mechanism is a push card connecting plate, and the cross-section of the push card connecting plate is "L"-shaped, and the side of the push card connecting plate is provided with an optical coupling sensor sheet that matches the photoelectric switch for induction; the push card assembly is provided with a push card mounting plate, a torsion spring, a push plate and a plug screw, and the push plate and the torsion spring are installed on the push card mounting plate by the plug screw, and the push plate extends toward the first-stage propulsion mechanism, and the push plate rotates unidirectionally toward the rear end.

[0010] The sample injection chamber door is installed on the casing through a rotating shaft and a torsion spring.

[0011] A sample injection baffle assembly is provided between the sample injection mechanism and the turntable mechanism to prevent the reagent card strip from falling into the mounting frame when the user misoperates. The sample injection baffle assembly is fixed to the mounting frame.

[0012] In some embodiments, the sample injection baffle assembly is arranged on the mounting shell. The sample injection baffle assembly is provided with a baffle frame, a baffle motor, a baffle photoelectric switch, a sleeve and a sample injection baffle. The baffle frame is arranged on the mounting frame and is located directly above the running track of the sample injection slot. The baffle motor is arranged on the baffle frame. The sample injection baffle is fixed to the rotating shaft of the baffle motor through the sleeve. The baffle photoelectric switch is arranged on the baffle frame and is matched with the sample injection baffle for induction.

[0013] In some embodiments, the turntable mechanism is provided with a turntable fixing plate, a bearing, a turntable, a turntable motor, a motor mounting frame, a turntable synchronous pulley, a turntable synchronous belt, heat insulation cotton, a heating pressing plate, a heating sheet, a temperature sensor, an annular limiting frame, a code disc opto-coupler bracket, a turntable photoelectric switch, a driven wheel shaft, a turntable driven wheel and a code disc. The bearing is connected to the turntable fixing plate. The annular limiting frame and the turntable are sequentially fixed on the upper side of the turntable fixing plate. A plurality of slots are annularly arranged on the turntable. The heating sheet is installed on the lower side of the turntable fixing plate through the heating pressing plate. The driven wheel shaft connects the turntable driven wheel and the bearing and is fixed. The heat insulation cotton is attached to the heating pressing plate. The temperature sensor is arranged in the turntable fixing plate. The turntable synchronous pulley is fixed on the turntable motor shaft. The turntable motor is installed on the turntable motor mounting frame. The turntable synchronous pulley and the turntable driven wheel are connected through the turntable synchronous belt. The code disc is fixed at the other end of the driven wheel shaft. The turntable photoelectric switch is fixed on the heating pressing plate through the code disc opto-coupler bracket and is matched with the code disc for induction.

[0014] The code disc is provided with an origin induction piece perpendicular to the code disc. The heating pressing plate is provided with an origin opto-coupler bracket to fix the origin photoelectric switch to be matched with the origin induction piece for induction. The origin photoelectric switch is used to locate the initial position of the turntable. The turntable photoelectric switch is used to locate the rotation angle of the turntable and corresponds one by one to the slots annularly arranged on the turntable.

[0015] In some embodiments, the sample ejection mechanism is provided with a sample ejection base, a sample ejection motor, a sample ejection synchronous pulley, a sample ejection driven wheel, a sample ejection linear guide rail, a sample ejection connecting block, a sample ejection synchronous belt, a sample ejection pressing plate, a sample ejection lever and a sample ejection photoelectric switch. A vertical motor plate is arranged on one side of the sample ejection base. The sample ejection motor is fixed on the motor plate. The sample ejection synchronous pulley is fastened on the rotating shaft of the sample ejection motor. The sample ejection driven wheel is arranged at the other end of the sample ejection base. The sample ejection linear guide rail is arranged on the base. The sample ejection connecting plate is arranged on the slider of the sample ejection linear guide rail. The sample ejection synchronous belt is arranged on the sample ejection synchronous pulley and the sample ejection driven wheel and is fixed on the sample ejection connecting plate through the sample ejection pressing plate. The sample ejection lever is arranged on the sample ejection connecting plate. The sample ejection photoelectric switch is arranged on the sample ejection base and is matched with the sample ejection lever for induction.

[0016] In some embodiments, the sample return chamber door is provided with cushion blocks, a base, a lead screw motor, a chamber door photoelectric switch, a chamber door bracket, and a chamber door. The cushion blocks are arranged at both ends of the base. The lead screw motor is fixed on the base. The chamber door bracket is arranged on the slider of the lead screw motor. The chamber door is arranged at one end of the chamber door bracket. The chamber door photoelectric switch is arranged on the base and is matched for induction with the other end of the chamber door bracket.

[0017] In some embodiments, the sample processing mechanism is provided with a motor mounting bracket, a lead screw motor, a suction cup backing plate, a suction cup mounting bracket, an optocoupler sensing sheet, a suction cup, a sample photoelectric switch, an air pump support plate, an air pump bracket, a buffer cotton, and an air pump. The lead screw motor is mounted on the motor mounting bracket. The suction cup backing plate is arranged on the slider of the lead screw motor. The suction cup mounting bracket and the optocoupler sensing sheet are fixed on the suction cup backing plate. The suction cup is fixed on the suction cup mounting bracket. The sample photoelectric switch is arranged on the side of the motor mounting bracket and is matched for induction with the optocoupler sensing sheet. The air pump is mounted on the air pump bracket. The air pump support plate is arranged at the front end of the air pump. The buffer cotton is arranged between the air pump and the air pump support plate. The air pump is connected to the suction cup through a hose to suck the detection hole position of the reagent strip sample, so that a relative pressure difference is formed between the detection hole of the reagent strip sample and the sample hole, so that the sample flows from the sample hole position through the processing structure of the reagent strip sample to the detection hole. Since there is a slight vibration when the air pump operates, the buffer cotton is added to support the air pump to prevent the tail end of the air pump from falling off due to long-term vibration.

[0018] In some embodiments, the detection module is provided with a fixed bracket, a lifting motor, a dual optical channel module, an optical module bracket, a detection sensing sheet, a detection photoelectric switch, and a buffer silica gel. The lifting motor is arranged on the fixed bracket. The dual optical channel module is fixed on the slider of the lifting motor through the optical module bracket. The detection sensing sheet is fixed on the optical module bracket. The detection photoelectric switch is arranged on the fixed bracket and is matched for induction with the detection sensing sheet. A buffer silica gel is arranged on the lower side of the dual optical channel module. Due to factors such as processing and assembly errors, there is a situation where the detection positions of the equipment are inconsistent between different batches. Therefore, by controlling the lifting motor, the consistency of the detection positions between batches can be achieved, thus ensuring the difference between stations. An electric crystal gasket is also arranged at the lower end of the optical installation shell to realize the locking of the buffer silica gel.

[0019] In some embodiments, the dual optical channel module is provided with an upper mold cover, an optical window, plano-convex lens a, plano-convex lens b, a filter, a PMT photomultiplier tube, a PMT mounting bracket, a lower mold cover, a dichroic mirror, and a laser. Vertical channels are provided on the upper mold cover and the lower mold cover, and mounting grooves are provided in the channels. The excitation light, plano-convex lens a, dichroic mirror, and optical window are sequentially arranged in the horizontal channel. The PMT photomultiplier tube, filter, plano-convex lens a, plano-convex lens b, dichroic mirror, and optical window are sequentially arranged in the vertical channel. The PMT photomultiplier tube is provided with a PMT mounting bracket and is fixed at the upper port of the vertical channel of the dual optical channel module.

[0020] In some embodiments, the detection module is further provided with a counting unit. The model of the counting unit is CH297. The counting unit can count the number of electrons received by the PMT. The PMT is connected to the counter signal line. The main board supplies 12V power to the counter through the power line and communicates with the main board through the serial line. When powered on, the main board is configured through the communication connection line with the counter. The gating parameters are set, and then the pulse pair resolution time is set according to the PMT specification parameters for its initialization configuration.

[0021] In some embodiments, the upper end of the longitudinal channel has a flared structure that is smaller at the top and larger at the bottom.

[0022] In some embodiments, the main control module. The operation of the main control module adopts timing control to ensure that the time and mixing times experienced by each reagent card inside are the same, ensuring that the reaction conditions of each reagent card are consistent. The operation of the turntable of the main control module adopts a servo motor, and the detection and positioning are carried out by the number of steps of the motor operation, reducing the data acquisition difference between different channels. The excitation light circuit adopts a constant current power supply design to improve the stability of the excitation light.

[0023] In some embodiments, a scanning module is provided on the side of the sample injection mechanism.

[0024] In a second aspect, the present application provides a detection method for a chemiluminescence detection device, including the following steps:

[0025] 1) Trigger the corresponding command through the human-machine interaction window and send it to the main control module to control the opening of the sample injection chamber door, and place the reagent strip sample with the added sample in the sample injection slot of the sample injection mechanism;

[0026] 2) Trigger the corresponding command through the human-machine interaction window to make the sample injection slot enter the chamber. The scanning module scans the reagent strip sample to confirm relevant information such as the item and batch number, and through the combined mechanical operation of the sample injection baffle assembly and the sample injection mechanism, the sample reagent strip sample enters the turntable mechanism.

[0027] 3) The turntable mechanism runs to the sample processing mechanism. After the sample is processed, the heating sheet of the turntable mechanism heats the turntable fixing plate, and the incubation of the sample is realized through heat conduction. During this period, the turntable always rotates left and right at a fixed frequency, driving the sample in the reagent strip sample to shake and continuously mixing;

[0028] 4) After the incubation time ends, the turntable mechanism runs to the darkroom of the detection module, and the optical detection unit inside the darkroom of the detection module runs downward in the Z direction to the detection position to detect the sample reagent strip sample;

[0029] 5) After the test is completed, the darkroom of the test module is reset, the turntable mechanism moves to the position of the sample return mechanism, the main control module controls the sample return chamber door to open, and the sample return mechanism returns the reagent strip sample to the external waste box, completing one round of testing.

[0030] Beneficial effects of the present invention: The device uses a photoluminescence method to provide energy for the corresponding reagent strip sample, so that the sample in the reagent produces a series of reactions, emitting a light wave with a wavelength of 610nm, and the detection of the sample is achieved by performing a specific conversion on the measured reading of the light intensity. At the same time, the device provides 16 detection channels. After the sample is added to the reagent strip sample, the reagent strip sample is automatically injected, and the sample is automatically processed, mixed, incubated and tested. After the test is completed, the sample reagent strip sample is automatically withdrawn to the waste card box, without the need for professional personnel to be on duty, simplifying the operation and the working hours of personnel. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Various other advantages and benefits will become apparent to those of ordinary skill in the art by reading the detailed description of the preferred embodiments below. The accompanying drawings are only for the purpose of illustrating the preferred embodiments and are not to be considered as limiting the present application. Moreover, the same reference numerals are used throughout the drawings to represent the same components. In the drawings:

[0032] Figure 1 This is a schematic diagram of the overall structure of some embodiments of the present application;

[0033] Figure 2 Another structural schematic diagram of some embodiments of the present application;

[0034] Figure 3 This is a schematic diagram of the internal structure of the detection device in some embodiments of the present application;

[0035] Figure 4 This is a schematic diagram of the structure of the feed bin door in some embodiments of the present application;

[0036] Figure 5 This is a schematic diagram of the structure of the sample injection mechanism of some embodiments of the present application;

[0037] Figure 6 Another schematic diagram of the sample injection mechanism of some embodiments of the present application;

[0038] Figure 7 Another schematic diagram of the sample injection mechanism of some embodiments of the present application;

[0039] Figure 8 This is a schematic diagram of the structure of the first-stage propulsion mechanism of some embodiments of the present application;

[0040] Figure 9 A schematic diagram of the structure of a secondary propulsion mechanism in some embodiments of the present application;

[0041] Figure 10 Explosion diagram of the primary propulsion mechanism for some embodiments of the present application;

[0042] Figure 11 Explosion diagram of the secondary propulsion mechanism for some embodiments of the present application;

[0043] Figure 12 Explosion diagram of the sample injection baffle assembly for some embodiments of the present application;

[0044] Figure 13 Schematic structural diagram of the turntable mechanism for some embodiments of the present application;

[0045] Figure 14 Explosion diagram of the turntable mechanism for some embodiments of the present application;

[0046] Figure 15 Partial schematic structural diagram of the turntable mechanism for some embodiments of the present application;

[0047] Figure 16 Explosion diagram of the sample processing mechanism for some embodiments of the present application;

[0048] Figure 17 Schematic structural diagram of the detection module for some embodiments of the present application;

[0049] Figure 18 Another schematic structural diagram of the detection module for some embodiments of the present application;

[0050] Figure 19 Another schematic structural diagram of the detection module for some embodiments of the present application;

[0051] Figure 20 Explosion diagram of the detection module for some embodiments of the present application;

[0052] Figure 21 Schematic structural diagram of the sample ejection mechanism for some embodiments of the present application;

[0053] Figure 22 Explosion diagram of the sample ejection mechanism for some embodiments of the present application;

[0054] Figure 23 Schematic diagram of the counting unit for some embodiments of the present application;

[0055] Figure 24 Schematic structural diagram of the sample ejection chamber door for some embodiments of the present application;

[0056] Figure 25 Explosion diagram of the sample ejection chamber door for some embodiments of the present application;

[0057] Figure 26Schematic diagram of the internal structure decomposition of the detection device in some embodiments of the present application;

[0058] Figure 27 Schematic diagram of the bell mouth structure of the detection module in some embodiments of the present application;

[0059] Figure 28 Circuit schematic diagram of the main control module in some embodiments of the present application;

[0060] Figure 29 Another circuit schematic diagram of the main control module in some embodiments of the present application;

[0061] Figure 30 Another circuit schematic diagram of the main control module in some embodiments of the present application.

[0062] The reference numerals in the specific embodiments are as follows:

[0063] Machine housing 1; mounting frame 11; sample inlet door 2; rotating shaft 21; torsion spring 22;

[0064] Sample outlet door 3; cushion block 31; base 32; lead screw motor 33; door photoelectric switch 34; door support 35; door 36;

[0065] Sample inlet mechanism 4; primary propulsion mechanism 41; secondary propulsion mechanism 42; push card assembly 43; push card mounting plate 431; torsion spring 432; push plate 433; set screw 434; drive assembly 44; bottom plate 441; mounting plate 4411; sample inlet motor 442; synchronous pulley 443; driven pulley 444; linear guide 445; sample inlet connecting plate 446; sample inlet groove 4461; push card connecting plate 4462; sample inlet opto-coupler sensing sheet 4463; synchronous belt 447; sample inlet pressing plate 448; sample inlet photoelectric switch 449;

[0066] Sample inlet baffle assembly 45; baffle frame 451; baffle motor 452; baffle photoelectric switch 453; sleeve 454; sample inlet baffle 455; scanning module 46;

[0067] Turntable mechanism 5; turntable fixing plate 51; bearing 52; turntable 53; turntable motor 54; turntable motor mounting frame 55; turntable synchronous pulley 56; turntable synchronous belt 57; heat insulation cotton 58; heating pressing plate 59; heating sheet 510; temperature sensor 511; annular limit frame 512; code disk opto-coupler support 513; turntable photoelectric switch 514; driven wheel shaft 515; turntable driven wheel 516; code disk 517; origin sensing sheet 518; origin photoelectric switch 519;

[0068] Sample Return Mechanism 6; Sample Return Base 61; Sample Return Motor 62; Sample Return Synchronous Pulley 63; Sample Return Driven Pulley 64; Sample Return Linear Guide 65; Sample Return Connecting Block 66; Sample Return Synchronous Belt 67; Sample Return Pressure Plate 68; Sample Return Poking Rod 69; Sample Return Photoelectric Switch 610; Main Control Module 7;

[0069] Sample Processing Mechanism 8; Motor Mounting Bracket 81; Lead Screw Motor 82; Suction Cup Pad 83; Suction Cup Mounting Bracket 84; Opto - Coupler Induction Sheet 85; Suction Cup 86; Sample Photoelectric Switch 87; Air Pump Support Plate 88; Air Pump Bracket 89; Buffer Cotton 810; Air Pump 811;

[0070] Detection Module 9; Fixed Bracket 91; Lifting Motor 92; Dual - Optical - Channel Module 93; Upper Mold Cover 931; Optical Window 932; Plano - Convex Lens a 933; Plano - Convex Lens b 934; Filter 935; PMT Photomultiplier Tube 936; PMT Mounting Bracket 937; Lower Mold Cover 938; Dichroic Mirror 939; Laser 9310; Counting Unit 9311; Optical Module Bracket 94; Detection Induction Sheet 95; Detection Photoelectric Switch 96; Buffer Silicone 97; Human - Machine Interaction Window 10. Detailed Implementation Manner

[0071] The embodiments of the technical solutions of the present application will be described in detail below with reference to the accompanying drawings. The following embodiments are only used to illustrate the technical solutions of the present application more clearly, so they are only examples and cannot be used to limit the protection scope of the present application.

[0072] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the description of the specification, claims and drawings of this application are intended to cover non - exclusive inclusion.

[0073] In the description of the embodiments of this application, technical terms such as "first" and "second" are only used to distinguish different objects and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity, specific order or primary - secondary relationship of the indicated technical features. In the description of the embodiments of this application, "a plurality of" means more than two unless otherwise specifically defined.

[0074] Reference to "embodiment" herein means that a particular feature, structure or characteristic described in connection with the embodiment can be included in at least one embodiment of this application. The phrase appears in various places in the specification and does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0075] In the description of the embodiments of the present application, the term "a plurality of" means two or more (including two). Similarly, "a plurality of groups" means two or more groups (including two groups), and "a plurality of sheets" means two or more sheets (including two sheets).

[0076] In the description of the embodiments of the present application, for technical terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., the orientation or positional relationship indicated is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the embodiments of the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation on the embodiments of the present application.

[0077] In the description of the embodiments of the present application, unless otherwise clearly specified and limited, technical terms such as "installation", "connection", "connection", "fixation", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can also be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to specific situations.

[0078] Refer to the attached Figure 1-27 As shown, taking the detection of a reagent strip sample as an example, a chemiluminescence detection device of the present application includes: a housing 1, a mounting rack 11 provided inside the housing 1, a sample loading chamber door 2 and a sample unloading chamber door 3 provided on one side of the housing 1, a human-machine interaction window 10 embedded in the front of the housing 1, and a sample loading mechanism 4, a turntable mechanism 5, a sample unloading mechanism 6, a main control module 7, a sample processing mechanism 8, and a detection module 9 provided on the mounting rack. The sample loading mechanism 4 is connected to the sample loading chamber door 2 and the turntable mechanism 5, the sample unloading mechanism 6 is connected to the turntable mechanism 5 and the sample unloading chamber door 3. The turntable mechanism 5 is provided inside the mounting rack 11. The sample loading mechanism 4, the sample processing mechanism 8, the detection module 9, and the sample unloading mechanism 6 are sequentially and annularly provided on the mounting rack 11 corresponding to the turntable mechanism. The main control module 7 is provided on the side of the mounting rack and is electrically connected to the detection module 9 and each mechanism.

[0079] The mounting rack 11 is composed of upper and lower mounting plates and mounting columns around. The mounting positions of each mechanism on the upper mounting plate and the detection module are correspondingly provided with slot holes.

[0080] The sample loading chamber door 2 and the sample unloading chamber door 3 are used to seal the openings for the reagent strip sample to be inserted and removed, so that the detection process is in a closed environment, avoiding the influence of the external environment and preventing the entry of dust and other substances. This setting method is superior to the existing open-top multi-detection slot setting method, and the open-top type is prone to foreign objects entering the detection device.

[0081] After the reagent strip sample enters, it is sent into the turntable mechanism 5 through the sample loading mechanism 4, then transported to the sample processing mechanism 8 through the turntable mechanism 5, and then enters the detection module 9 for detection. After the detection is completed, it enters the sample unloading mechanism 6, and the reagent strip sample is transferred to the waste box from the sample unloading chamber door 3.

[0082] The detection process is automatically controlled and completed by the main control module 7, without the need for professional personnel to be on duty, simplifying the operation and the working hours of personnel, reducing the interference of personnel, and improving the detection efficiency and accuracy.

[0083] In some embodiments, the sample loading mechanism 4 is provided with a two-stage propulsion mechanism. The first-stage propulsion mechanism 41 and the second-stage propulsion mechanism 42 are connected by a push card assembly 43. The first-stage propulsion mechanism 41 is arranged on the mounting shell, and the second-stage propulsion mechanism 42 is arranged on the mounting frame 11. The first-stage propulsion mechanism 41 and the second-stage propulsion mechanism 42 are provided with a drive assembly 44. As Figure 5-11 。

[0084] The setting of the two-stage propulsion mechanism can reduce the running length of the sample loading structure, reduce the overall volume of the instrument, and make the instrument structure more compact, which is beneficial to the stability of the equipment operation. The setting of the two-stage propulsion mechanism facilitates sample loading and sample transmission. The drive assembly 44 can achieve linear motion through belt transmission, cylinder transmission, screw motor, etc. After the first-stage propulsion mechanism 41 transports the reagent strip sample to the push card assembly 43, the second-stage propulsion mechanism 42 acts to further push the reagent strip sample forward to complete the sample loading.

[0085] The driving assembly 44 is provided with a base plate 441, a sample injection motor 442, a synchronous wheel 443, a driven wheel 444, a linear guide 445, a connecting plate 446, a synchronous belt 447, a sample injection pressure plate 448 and a sample injection photoelectric switch 449. A vertical mounting plate 4411 is provided on one side of the base plate 441. The sample injection motor 442 is fixed on the mounting plate 4411. The synchronous wheel 443 is fastened to the rotating shaft of the sample injection motor 442. The driven wheel 444 is arranged at the other end of the base plate 441. The linear guide 445 is arranged on the base plate 441. The connecting plate 446 is arranged on the slider of the linear guide 445. The synchronous belt 447 is arranged on the synchronous wheel 443 and the driven wheel 444 and is fixed to the connecting plate 446 through the sample injection pressure plate 448. The sample injection photoelectric switch 449 is respectively arranged at the end of the primary propulsion mechanism 41 and the front end of the secondary propulsion mechanism 42 to control the reagent. The strip samples are transferred between the primary propulsion mechanism 41 and the secondary propulsion mechanism 42; the connection plate 446 of the primary propulsion mechanism 41 is matched with the sample injection photoelectric switch 449 for induction, and a sample injection slot 4461 is also provided at the front end of the connection plate 446 of the primary propulsion mechanism 41; the connection plate 446 of the secondary propulsion mechanism 42 is a push card connection plate 4462, and the push card connection plate 4462 has an "L"-shaped cross section; a sample injection optical coupling sensor sheet 4463 is provided on the side of the push card connection plate 4462 for induction matching with the sample injection photoelectric switch 449; the push card assembly 43 is provided with a push card mounting plate 431, a torsion spring 432, a push plate 433 and a plug screw 434; the push plate 433 and the torsion spring 432 are installed on the push card mounting plate 431 by means of the plug screw 434; the push plate 433 extends toward the primary propulsion mechanism 41, and the push plate 433 rotates unidirectionally toward the rear end. Figure 5-11 .

[0086] The present application arranges a synchronous belt in a limited detection device to drive the sample injection slot to realize transmission, in order to alleviate the impact force of transmission, so that the transmission of the reagent strip sample is more stable while ensuring the speed.

[0087] The driving assembly 44 of the first-stage propulsion mechanism 41 is installed on the annular limit frame 512, and the driving assembly 44 of the second-stage propulsion mechanism 42 is installed on the upper mounting plate of the mounting frame 11. The two-stage propulsion mechanisms are arranged opposite to each other, and a conveying channel is formed in the middle. The injection slot 4461 and the card pushing assembly 43 are located in the channel, and the card pushing assembly 43 receives the reagent strip sample conveyed halfway by the injection slot 4461.

[0088] The push plate 433 is limited by the push plate mounting plate 431 in the initial state, and the push plate 433 is located in the channel. When the sample injection slot 4461 passes by, it rotates and avoids under the push of the reagent strip sample rack. The sample injection slot 4461 is reset by the rear push plate 433, and then the secondary propulsion mechanism 42 is activated, and the push plate 433 further propels the reagent strip sample forward.

[0089] In some embodiments, the sample injection chamber door 2 is mounted on the housing 1 through a rotating shaft 21 in cooperation with a torsion spring 22. When the sample injection chamber door 2 moves outward from the sample injection slot 4461, it is pushed open, and the reagent strip sample can be placed into the sample injection slot 4641. When the sample injection slot 4461 moves inward, the sample injection chamber door 2 closes under the action of the torsion spring 22. As Figure 2 , 3 .

[0090] In some embodiments, a sample injection baffle assembly 45 is provided between the sample injection mechanism 4 and the turntable mechanism 5, and the sample injection baffle assembly 45 is fixed to the mounting frame 11. The sample injection baffle assembly 45 is located directly above the running track of the sample injection slot to prevent the reagent card strip from falling into the mounting frame when the user operates by mistake. As Figure 3 .

[0091] In some embodiments, the sample injection baffle assembly 45 is provided on the mounting shell. The sample injection baffle assembly 45 is provided with a baffle frame 451, a baffle motor 452, a baffle photoelectric switch 453, a sleeve 454, and a sample injection baffle 455. The baffle frame 451 is provided on the mounting frame 11, the baffle motor 452 is provided on the baffle frame 451, the sample injection baffle 451 is fixed to the rotating shaft of the baffle motor 452 through the sleeve 454, and the baffle photoelectric switch 453 is provided on the baffle frame 451 and matches the sample injection baffle 455 for induction. As Figure 3 , 12 .

[0092] In some embodiments, the turntable mechanism 5 is provided with a turntable fixing plate 51, a bearing 52, a turntable 53, a turntable motor 54, a turntable motor mounting frame 55, a turntable synchronous pulley 56, a turntable synchronous belt 57, a heat preservation cotton 58, a heating pressing plate 59, a heating sheet 510, a temperature sensor 511, an annular limiting frame 512, a code disk opto-coupler bracket 513, a turntable photoelectric switch 514, a driven wheel shaft 515, a turntable driven wheel 516, and a code disk 517. The bearing 52 is connected to the turntable fixing plate 51, the annular limiting frame 512 and the turntable 53 are sequentially fixed on the upper side of the turntable fixing plate 51. A plurality of slots are annularly arranged on the turntable 53. The heating sheet 510 is installed on the lower side of the turntable fixing plate 51 through the heating pressing plate 59. The driven wheel shaft 515 connects the turntable driven wheel 516 and the bearing 52 and is fixed. The heat preservation cotton 58 is attached to the heating pressing plate 59. The temperature sensor 511 is arranged in the side mounting holes of the turntable fixing plate 51 and is arranged at two places front and back at 180°. The turntable synchronous pulley 56 is fixed on the shaft of the turntable motor 54. The turntable motor 54 is installed on the turntable motor mounting frame 55. The turntable synchronous pulley 56 and the turntable driven wheel 516 are connected through the turntable synchronous belt 57. The code disk 517 is fixed to the other end of the driven wheel shaft 515. The turntable photoelectric switch 514 is fixed to the heating pressing plate 59 through the code disk opto-coupler bracket 513 and matches the code disk 517 for induction. AsFigure 3 , 13 -15, 26.

[0093] The turntable mechanism 5 can accurately record the information of each slot and control the temperature conditions for detecting the actual strip. The annular limiting frame 512 is provided with notches corresponding to each mechanism, and the annular limiting frame 512 is also provided with a frame cover, and working slots are provided on the frame cover corresponding to each mechanism and the detection module.

[0094] An origin sensing piece 518 perpendicular to the code disc 517 is provided on the code disc 517, and an origin optocoupler bracket for fixing the origin optoelectronic switch 519 is provided on the heating pressing plate 59 to match and sense the origin sensing piece 518. The origin optoelectronic switch 519 is used to locate the initial position of the turntable 53, and the turntable optoelectronic switch 514 is used to locate the rotation angle of the turntable 53, which corresponds one by one to the slots annularly arranged on the turntable 53.

[0095] In some embodiments, the sample return mechanism 6 is provided with a sample return base 61, a sample return motor 62, a sample return synchronous pulley 63, a sample return driven pulley 64, a sample return linear guide 65, a sample return connecting block 66, a sample return synchronous belt 67, a sample return pressing plate 68, a sample return lever 69 and a sample return optoelectronic switch 610. A vertical motor plate is provided on one side of the sample return base 61, the sample return motor 62 is fixed on the motor plate, the sample return synchronous pulley 63 is fastened to the rotating shaft of the sample return motor 62, the sample return driven pulley 64 is arranged at the other end of the sample return base 61, the linear guide 65 is arranged on the sample return base 61, the sample return connecting plate 66 is arranged on the slider of the sample return linear guide 65, the sample return synchronous belt 67 is arranged on the sample return synchronous pulley 63 and the sample return driven pulley 64 and fixed to the sample return connecting plate 66 through the sample return pressing plate 68, the sample return lever 69 is arranged on the sample return connecting plate 66, and the sample return optoelectronic switch 610 is arranged on the sample return base 61 to match and sense the sample return lever 69. As Figure 20-21 .

[0096] In some embodiments, the sample return chamber door 3 is provided with a cushion block 31, a base 32, a lead screw motor 33, a chamber door optoelectronic switch 34, a chamber door bracket 35 and a chamber door 36. The cushion block 31 is arranged at both ends of the base 32, the lead screw motor 33 is fixed on the base 32, the chamber door bracket 35 is arranged on the slider of the lead screw motor 33, the chamber door 36 is arranged at one end of the chamber door bracket 35, and the chamber door optoelectronic switch 34 is arranged on the base 32 to match and sense the other end of the chamber door bracket 35. As Figure 24-25 .

[0097] In some embodiments, the sample processing mechanism 8 is provided with a motor mounting bracket 81, a lead screw motor 82, a suction cup backing plate 83, a suction cup mounting bracket 84, an optocoupler sensing piece 85, a suction cup 86, a sample optoelectronic switch 87, an air pump support plate 88, an air pump bracket 89, a buffer cotton 810, and an air pump 811. The lead screw motor 82 is mounted on the motor mounting bracket 81. The suction cup backing plate 83 is arranged on the slider of the lead screw motor 82. The suction cup mounting bracket 84 and the optocoupler sensing piece 85 are fixed on the suction cup backing plate 83. The suction cup 86 is fixed on the suction cup mounting bracket 84. The sample optoelectronic switch 87 is arranged on the side of the motor mounting bracket 81 to match and sense with the optocoupler sensing piece 85. The air pump 811 is mounted on the air pump bracket 89. The air pump 811 is connected to the suction cup 86 through a hose to suck the detection hole position of the reagent strip sample, so that a relative pressure difference is formed between the detection hole of the reagent strip sample and the sample hole, so that the sample flows from the sample hole position through the processing structure of the reagent strip sample to the detection hole. The air pump support plate 88 is arranged at the front end of the air pump 811. The buffer cotton 810 is arranged between the air pump 811 and the air pump support plate 88. Since there is a slight vibration when the air pump 811 operates, the situation of the tail end of the air pump 811 falling off occurred in the previous models. Therefore, the buffer cotton 810 is added to support the air pump to prevent it from falling off due to long-term vibration. As Figure 3 , 16 , 26.

[0098] In some embodiments, the detection module 9 is provided with a fixed bracket 91, a lifting motor 92, a dual optical channel module 93, an optical module bracket 94, a detection sensing piece 95, a detection optoelectronic switch 96, and a buffer silica gel 97. The lifting motor 92 is arranged on the fixed bracket 91. The dual optical channel module 93 is fixed on the slider of the lifting motor 92 through the optical module bracket 94. The detection sensing piece 95 is fixed on the optical module bracket 94. The detection optoelectronic switch 96 is arranged on the fixed bracket 91 to match and sense with the detection sensing piece 95. A buffer silica gel 97 is arranged on the lower side of the dual optical channel module 93. As Figure 3 , 17 -20, 26. Due to factors such as processing and assembly errors, there is a situation where the detection positions of the equipment are inconsistent among different batches. Therefore, by controlling the lifting motor 92, the consistency of the detection positions among batches can be achieved, thereby ensuring the inter-table difference.

[0099] In some embodiments, the dual optical channel module 93 is provided with an upper mold cover 931, an optical window 932, plano-convex lens a 933, plano-convex lens b 934, a filter 935, a PMT photomultiplier tube 936, a PMT mounting bracket 937, a lower mold cover 938, a dichroic mirror 939, and a laser 9310. There are two plano-convex lenses a 933. The upper mold cover 931 and the lower mold cover 938 are provided with vertical channels, and the channels are provided with mounting grooves. The laser 9310, the plano-convex lens a 933, the dichroic mirror 939, and the optical window 932 are sequentially arranged in the horizontal channel, and the PMT photomultiplier tube 936, the filter 935, the plano-convex lens a 933, the plano-convex lens b 934, the dichroic mirror 939, and the optical window 932 are sequentially arranged in the vertical channel; the PMT photomultiplier tube 936 is fixed to the upper port of the vertical channel of the dual optical channel module 93 by the PMT mounting bracket 937. As Figure 17-20 。

[0100] There are two plano-convex lenses a 933. The dual optical channel module 93 has an excitation optical path and a light emission optical path. The horizontal channel is the excitation optical path, and the vertical channel is the light emission optical path.

[0101] In some embodiments, the detection module 9 is further provided with a counting unit 9311. As Figure 3 、 23 、26. The model of the counting unit 9311 is CH297. The counting unit 9311 can count the number of electrons received by the PMT; setting method: when powered on, the main board is configured through the communication connection line with the counter; set the gating parameters, and then set the pulse pair resolution time according to the PMT specification parameters, and perform initialization configuration; connection method: the PMT is connected to the counter signal line, the main board supplies 12V power to the counter through the power line, and communicates with the main board through the serial port line.

[0102] In some embodiments, the upper end of the vertical channel has a flared structure that is smaller at the top and larger at the bottom. The flared structure cooperates with the focusing effect of the plano-convex lens a 933 to maximize the concentration of optical energy on the effective area of the detection window of the multiplier tube. As Figure 27 。

[0103] In some embodiments, the main control module 7. As Figure 28-30 。The main control module 7 operates with timing control to ensure that the time and the number of mixing times experienced by each reagent card inside are the same, and to ensure that the reaction conditions of each reagent card are consistent; the main control module 7 uses a servo motor for the turntable operation, and detects and locates by the number of steps of the motor operation to reduce the data acquisition difference between different channels; the excitation light circuit adopts a constant current power supply design to improve the stability of the excitation light.

[0104] In some embodiments, a scanning module 46 is provided on the side of the sample injection mechanism 4. The model of the scanning module 46 is the Vancoo VM2290 embedded two-dimensional imaging module, which adopts advanced CMOS imaging recognition technology and an intelligent image recognition system, and is equipped with a 300,000-pixel high-definition camera. It has excellent reading performance and can easily read the barcodes on the reagent strips. The front part of the module includes a lighting lamp, and the aiming lamp camera is used for barcode scanning. The bottom is fixed to the frame through two M1.6 threaded holes.

[0105] In some embodiments, the human-computer interaction window 10 at least includes a main menu display area, a to-be-tested sample display area, a channel display area, and a test result list. The main menu includes test, calibration, quality control, record, setting, and account windows. The to-be-tested sample display area at least displays the sample number, name, age, gender, sample ID, doctor, and department. The channel display area at least displays the channel number and status. The test result list at least includes a selection box, serial number, name, sample ID, reagent batch number, test result, report time, query, print, data export, and export.

[0106] In a second aspect, the present application provides a chemiluminescence detection device detection method for a reagent strip sample, including the following steps:

[0107] 1) Trigger (which can be achieved by clicking, mouse manipulation, touch screen, etc.) a corresponding command 1 through the human-computer interaction window 10, and send it to the main control module 7 to control the opening of the sample injection chamber door 2, and place the reagent strip sample with the added sample in the sample injection slot 4461 of the sample injection mechanism 4;

[0108] 2) Trigger a corresponding command 2 through the human-computer interaction window 10 to make the sample injection slot 4461 enter the chamber. The scanning module 46 scans the reagent strip sample to confirm relevant information such as the item and batch number, and through the combined mechanical operation of the sample injection baffle assembly 45 and the sample injection mechanism 4, the sample reagent strip sample enters the turntable mechanism 5.

[0109] 3) The turntable mechanism 5 runs to the sample processing mechanism 8 to complete the processing of the sample. The heating sheet 510 of the turntable mechanism 5 heats the turntable fixing plate 51, and realizes the incubation of the sample through heat conduction. During this period, the turntable 53 always rotates left and right at a fixed frequency, driving the sample in the reagent strip sample to shake and continuously mix;

[0110] 4) After the incubation is completed, the turntable mechanism 5 runs to the darkroom of the detection module 9. The optical detection unit inside the darkroom of the detection module 9 runs downward in the Z direction to the detection position to detect the sample reagent strip sample;

[0111] 5) After the detection is completed, the detection module 9 resets the darkroom, the turntable mechanism 5 runs to the position of the sample ejection mechanism 6, the main control module 7 controls the opening of the sample ejection bin door 3, and the sample ejection mechanism 6 ejects the reagent strip sample into the external waste box to complete one round of detection.

[0112] The whole device is in the form of a turntable, and there are 16 detection channels arranged on the turntable 53. In order to achieve the rapid switching of the turntable 53 between different channels while ensuring the accurate rotation of the turntable 53, a servo motor is used to match the synchronous belt for motion control.

[0113] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present application, and they should all be covered by the scope of the claims and the description of the present application. In particular, as long as there is no structural conflict, the technical features mentioned in each embodiment can be combined in any way. The present application is not limited to the specific embodiments disclosed in the text, but includes all technical solutions falling within the scope of the claims.

Claims

1. A chemiluminescence detection device, characterized in that, include: A housing, a mounting frame arranged in the housing, a sample feeding chamber door and a sample return chamber door arranged on one side of the housing, a human-machine interaction window inlaid on the front of the housing, and a sample feeding mechanism, a turntable mechanism, a sample return mechanism, a main control module, a sample processing mechanism and a detection module arranged on the mounting frame, wherein the sample feeding mechanism is connected to the sample feeding chamber door and the turntable mechanism, the sample return mechanism is connected to the turntable mechanism and the sample return chamber door, the turntable mechanism is arranged in the mounting frame, the sample feeding mechanism, the sample processing mechanism, the detection module and the sample return mechanism are arranged in a circular manner on the mounting frame in sequence corresponding to the turntable mechanism, and the main control module is arranged on the side of the mounting frame and is connected to the detection module and various mechanisms by electrical signals; An injection baffle assembly is provided between the injection mechanism and the turntable mechanism, and the injection baffle assembly is fixed to the mounting frame; The sample injection baffle assembly is arranged on the mounting shell, and the sample injection baffle assembly is provided with a baffle frame, a baffle motor, a baffle photoelectric switch, a sleeve and a sample injection baffle. The baffle frame is arranged on the mounting frame, the baffle motor is arranged on the baffle frame, the sample injection baffle is fixed to the rotating shaft of the baffle motor through the sleeve, and the baffle photoelectric switch is arranged on the baffle frame to match the sample injection baffle for induction; The sample injection mechanism is provided with a two-stage propulsion mechanism, and the first-stage propulsion mechanism and the second-stage propulsion mechanism are connected by a card pushing assembly; the first-stage propulsion mechanism and the second-stage propulsion mechanism are provided with a driving assembly; The driving assembly is provided with a base plate, a sample injection motor, a synchronous wheel, a driven wheel, a linear guide rail, a connecting plate, a synchronous belt, a sample injection pressing plate and a sample injection photoelectric switch; The connecting plate is arranged on the slider of the linear guide rail, and the sample injection photoelectric switch is respectively arranged at the end of the first-level propulsion mechanism and the front end of the second-level propulsion mechanism to control the reagent strip sample to complete the acceptance and transmission between the first-level propulsion mechanism and the second-level propulsion mechanism; the connecting plate of the first-level propulsion mechanism is matched with the sample injection photoelectric switch for induction, and the front end of the connecting plate of the first-level propulsion mechanism is also provided with a sample injection slot, and the connecting plate of the second-level propulsion mechanism is a push card connecting plate, and the side of the push card connecting plate is provided with a sample injection optical coupling sensor sheet that matches the sample injection photoelectric switch for induction; the push card assembly is provided with a push card mounting plate, a torsion spring, a push plate and a plug screw, and the push plate and the torsion spring are installed on the push card mounting plate by the plug screw, and the push plate extends to the first-level propulsion machine, and the push plate rotates unidirectionally toward the rear end; The two-stage propulsion mechanisms are arranged opposite to each other, forming a conveying channel in the middle. The sample injection slot and the card pushing assembly are located in the channel, and the card pushing assembly receives the reagent strip sample that is conveyed halfway by the sample injection slot.

2. A chemiluminescence detection device according to claim 1, wherein, The turntable mechanism is provided with a turntable fixing plate, a bearing, a turntable, a turntable motor, a turntable motor mounting bracket, a turntable synchronous pulley, a turntable synchronous belt, heat insulation cotton, a heating pressing plate, a heating sheet, a temperature sensor, an annular limiting frame, a code disk optocoupler bracket, a turntable photoelectric switch, a driven wheel shaft, a turntable driven wheel, and a code disk. The bearing is connected to the turntable fixing plate. The annular limiting frame and the turntable are sequentially fixed on the upper side of the turntable fixing plate. A plurality of slots are annularly arranged on the turntable. The heating sheet is installed on the lower side of the turntable fixing plate through the heating pressing plate. The driven wheel shaft connects the turntable driven wheel and the bearing and is fixed. The heat insulation cotton is attached to the heating pressing plate. The temperature sensor is arranged inside the turntable fixing plate. The turntable synchronous pulley is fixed on the turntable motor shaft. The turntable motor is installed on the turntable motor mounting bracket. The turntable synchronous pulley and the turntable driven wheel are connected by the turntable synchronous belt. The code disk is fixed at the other end of the driven wheel shaft. The turntable photoelectric switch is fixed on the heating pressing plate through the code disk optocoupler bracket and matches and senses the code disk.

3. A chemiluminescence detection device according to claim 2, characterized in that, The code disk is provided with an origin sensing piece perpendicular to the code disk. The heating pressing plate is provided with an origin optocoupler bracket to fix the origin photoelectric switch to match and sense the origin sensing piece.

4. A chemiluminescence detection device according to claim 1, wherein The sample returning mechanism is provided with a sample returning base, a sample returning motor, a sample returning synchronous pulley, a sample returning driven wheel, a sample returning linear guide rail, a sample returning connecting block, a sample returning synchronous belt, a sample returning pressing plate, a sample returning lever, and a sample returning photoelectric switch. A vertical motor plate is provided on one side of the sample returning base. The sample returning motor is fixed on the motor plate. The sample returning synchronous pulley is fastened on the rotating shaft of the sample returning motor. The sample returning driven wheel is arranged at the other end of the sample returning base. The sample returning linear guide rail is arranged on the sample returning base. The sample returning connecting plate is arranged on the slider of the sample returning linear guide rail. The sample returning synchronous belt is arranged on the sample returning synchronous pulley and the sample returning driven wheel and is fixed on the sample returning connecting plate through the sample returning pressing plate. The sample returning lever is arranged on the sample returning connecting plate. The sample returning photoelectric switch is arranged on the sample returning base to match and sense the sample returning lever.

5. A chemiluminescence detection device according to claim 1, wherein The sample returning chamber door is provided with a cushion block, a base, a lead screw motor, a chamber door photoelectric switch, a chamber door bracket, and a chamber door. The cushion blocks are arranged at both ends of the base. The lead screw motor is fixed on the base. The chamber door bracket is arranged on the slider of the lead screw motor. The chamber door is arranged at one end of the chamber door bracket. The chamber door photoelectric switch is arranged on the base to match and sense the other end of the chamber door bracket.

6. The chemiluminescence detection device according to claim 1, wherein The sample processing mechanism is provided with a motor mounting bracket, a lead screw motor, a suction cup backing plate, a suction cup mounting bracket, an optocoupler sensing piece, a suction cup, a sample photoelectric switch, an air pump support plate, an air pump bracket, buffer cotton, and an air pump. The lead screw motor is installed on the motor mounting bracket. The suction cup backing plate is arranged on the slider of the lead screw motor. The suction cup mounting bracket and the optocoupler sensing piece are fixed on the suction cup backing plate. The suction cup is fixed on the suction cup mounting bracket. The sample photoelectric switch is arranged on the side of the motor mounting bracket to match and sense the optocoupler sensing piece. The air pump is installed on the air pump bracket. The air pump support plate is arranged at the front end of the air pump. The buffer cotton is arranged between the air pump and the air pump support plate.

7. A chemiluminescence detection device according to claim 1, characterized in that, The detection module is provided with a fixing frame, a lifting motor, a dual optical channel module, an optical module bracket, a detection induction sheet, a detection optoelectronic switch, and a buffer silica gel. The lifting motor is arranged on the fixing frame. The dual optical channel module is fixed to the slider of the lifting motor through the optical module bracket. The detection induction sheet is fixed to the optical module bracket. The detection optoelectronic switch is arranged on the fixing frame and is matched with the detection induction sheet for induction.

8. The detection method of a chemiluminescence detection device according to any one of claims 1-7, comprising the following steps: 1) Trigger a corresponding command through the human-computer interaction window, send it to the main control module, control the opening of the sample injection chamber door, and place the sample with the added sample in the sample injection slot of the sample injection mechanism; 2) Trigger a corresponding command through the human-computer interaction window to make the sample injection slot enter the chamber. The scanning module scans the sample to confirm the relevant information of the item and batch number. Through the combined mechanical operation of the sample injection baffle assembly and the sample injection mechanism, the sample sample enters the turntable mechanism; 3) The turntable mechanism runs to the sample processing mechanism. After the sample is processed, the heating sheet of the turntable mechanism heats the turntable fixing plate, and the incubation of the sample is realized through heat conduction. During this period, the turntable always rotates left and right at a fixed frequency, driving the sample in the sample to shake and continuously mixing; 4) After the incubation time ends, the turntable mechanism runs to the darkroom of the detection module. The optical detection unit inside the darkroom of the detection module moves downward in the Z direction to the detection position to detect the sample sample; 5) After the detection is completed, the darkroom of the detection module resets. The turntable mechanism runs to the position of the sample ejection mechanism. The main control module controls the opening of the sample ejection chamber door, and the sample ejection mechanism ejects the sample to the external waste box to complete a round of detection.

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