A push piece dyeing machine, a control method thereof and a sample detection device

Abstract

The application provides a slide pushing and dyeing machine, a control method thereof and a sample detection device. The slide pushing and dyeing machine comprises: a sample adding unit configured to add a sample to a slide; an image acquisition unit configured to acquire a sample image of the sample on the slide; a slide pushing unit configured to spread the sample on the slide; a dyeing unit configured to dye the slide; and a controller in communication connection with the image acquisition unit, the slide pushing unit and the dyeing unit. The controller is configured to determine a contact angle of the sample and the slide according to the sample image, determine a slide pushing parameter of the slide pushing unit according to the contact angle, control the slide pushing unit to push the slide according to the slide pushing parameter, and control the dyeing unit to dye the slide. The contact angle reflects the viscosity of the sample, so that the slide pushing unit can perform a slide pushing operation according to the slide pushing parameter corresponding to the viscosity of the sample, and the high-quality standard of a smear formed by spreading the sample on the slide is ensured.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, in particular to a push piece dyeing machine, a control method thereof and a sample detection device. BACKGROUND

[0002] In the diagnosis and treatment of diseases, a sample is smeared on a slide to form a smear by a push piece, and then the smear is dyed to complete the preparation, and finally microscopic detection is performed to obtain a detection result, which provides a reference basis for doctors to diagnose diseases. For example, microscopic detection of a smear is a basic method of liquid cytology detection, which is widely used, and has great value for the diagnosis of various liquid diseases.

[0003] In the prior art, in order to ensure that the smear has high quality standards such as appropriate thickness and uniform distribution, the push piece is generally operated at a set push piece parameter by setting push piece parameters such as push piece angle and push piece speed. However, when the viscosity of the sample is different, if the same push piece parameter is used for push piece operation, the smear may not meet the high quality standards. SUMMARY

[0004] The present application mainly provides a push piece dyeing machine, a control method thereof and a sample detection device, which can ensure the high quality standards of the smear formed by smearing the sample on the slide.

[0005] To solve the above technical problems, one technical solution adopted by the present application is to provide a push piece dyeing machine, which comprises: a sample adding unit for adding a sample to a slide; an image acquisition unit for acquiring a sample image of the sample on the slide; a push piece unit for smearing the sample on the slide; a dyeing unit for dyeing the slide; a controller in communication connection with the image acquisition unit, the push piece unit and the dyeing unit, the controller being configured to determine a contact angle of the sample with the slide according to the sample image, determine a push piece parameter of the push piece unit according to the contact angle, control the push piece unit to push the slide according to the push piece parameter, and control the dyeing unit to dye the slide.

[0006] In one specific embodiment, the controller is configured to determine the viscosity of the sample according to the contact angle, and determine the push piece parameter according to the viscosity of the sample.

[0007] In one specific embodiment, the controller is further configured to determine whether the sample amount on the slide meets a push piece standard, control the sample adding unit to extract part of the sample on the slide when the sample amount is determined to be higher than the push piece standard, and control the sample adding unit to supplement the sample on the slide when the sample amount is determined to be lower than the push piece standard.

[0008] In an embodiment, the slide dyeing machine further comprises a weight detection unit, which is in communication with the controller and is configured to detect the weight of the slide, and the controller is configured to determine whether the sample amount on the slide meets the slide pushing standard according to the weight of the slide.

[0009] In an embodiment, the image acquisition unit is arranged on the side of the slide.

[0010] In an embodiment, the side of the slide is further provided with a background plate, which is arranged opposite to the image acquisition unit.

[0011] In an embodiment, the slide dyeing machine further comprises a conveying unit for conveying the slide, and a slide pushing position is arranged along the movement track of the slide on the conveying unit, and the slide pushing unit is configured to apply the sample on the slide at the slide pushing position.

[0012] In an embodiment, the sample adding unit is configured to add the sample on the first slide, and the slide dyeing machine further comprises a detection carrying mechanism configured to carry the first slide to allow the image acquisition unit to acquire a sample image of the sample on the first slide, and the controller is configured to determine a contact angle of the sample and the first slide according to the sample image, determine a slide pushing parameter of the slide pushing unit according to the contact angle, and control the slide pushing unit to apply the sample on the first slide at the slide pushing position according to the slide pushing parameter; or the sample adding unit is configured to add the same sample on the first slide and the second slide, and the slide dyeing machine further comprises a detection carrying mechanism configured to carry the first slide to allow the image acquisition unit to acquire a sample image of the sample on the first slide, and the controller is configured to determine a contact angle of the sample and the first slide according to the sample image, determine a slide pushing parameter of the slide pushing unit according to the contact angle, and control the slide pushing unit to apply the sample on the second slide at the slide pushing position according to the slide pushing parameter.

[0013] In an embodiment, the image acquisition unit is configured to acquire a sample image of the sample on the slide on the conveying unit, and the controller is configured to control the conveying unit to move the slide to the slide pushing position after the image acquisition unit finishes acquiring the sample image.

[0014] To solve the above technical problems, another technical solution adopted by the present application is to provide a sample detection device, which comprises a detection device and the push slice staining machine, the detection device is used for detecting the sample and is in communication connection with the controller, the controller is used for receiving the detection result of the detection device and judging whether the push slice parameters of the sample exist in the database, and the controller is further used for acquiring the push slice parameters of the sample when the push slice parameters of the sample exist in the database, and controlling the image acquisition unit to acquire the sample image of the sample on the slide when the push slice parameters of the sample do not exist in the database.

[0015] To solve the above technical problems, another technical solution adopted by the present application is to provide a control method of a push slice staining machine, the push slice staining machine comprising a sample adding unit, an image acquisition unit, a push slice unit and a staining unit, the control method comprising: controlling the sample adding unit to add an ex vivo biological sample to a slide; controlling the image acquisition unit to acquire an image of the ex vivo biological sample on the slide to obtain a microscopic image; determining a contact angle of the ex vivo biological sample and the slide according to the microscopic image; determining push slice parameters of the push slice unit according to the contact angle; and controlling the push slice unit to push the slide according to the push slice parameters, and controlling the staining unit to stain the slide.

[0016] The present application has the following beneficial effects: Different from the prior art, the push slice staining machine provided by the embodiments of the present application comprises a sample adding unit, an image acquisition unit, a push slice unit, a staining unit and a controller, the sample adding unit is used for adding a sample to a slide, the image acquisition unit is used for acquiring a sample image of the sample on the slide, the push slice unit is used for coating the sample on the slide, the staining unit is used for staining the slide, the controller is in communication connection with the image acquisition unit, the push slice unit and the staining unit, the controller is used for determining a contact angle of the sample and the slide according to the sample image, determining push slice parameters of the push slice unit according to the contact angle, controlling the push slice unit to push the slide according to the push slice parameters, and controlling the staining unit to stain the slide, the contact angle reflects the viscosity of the sample, so that the push slice unit can perform the push slice operation according to the push slice parameters corresponding to the viscosity of the sample, and the high-quality standard of the smear formed by coating the sample on the slide is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the following embodiment description will be briefly introduced, and obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without any creative effort.

[0018] Figure 1 is a schematic diagram of the three-dimensional assembly structure of an embodiment of the pusher device provided in the application;

[0019] Figure 2 is Figure 1 is a schematic diagram of the three-dimensional exploded structure of the pusher device in the application;

[0020] Figure 3 is Figure 1 is a control schematic diagram of the pusher device in the application;

[0021] Figure 4 is Figure 2 is a schematic diagram of the three-dimensional structure of the sample adding unit in the application;

[0022] Figure 5 is Figure 4 is a schematic diagram of the principle structure of the sample adding needle in the application;

[0023] Figure 6 is Figure 1 is a position schematic diagram of an embodiment of the pusher device in the application;

[0024] Figure 7 is Figure 1 is a position schematic diagram of another embodiment of the pusher device in the application;

[0025] Figure 8 is Figure 2 is a schematic diagram of the three-dimensional structure of the pusher unit in the application;

[0026] Figure 9 is Figure 2 is a schematic diagram of the three-dimensional structure of the conveying unit in the application;

[0027] Figure 10 is Figure 2 is a schematic diagram of the three-dimensional structure of the carrying unit in the application;

[0028] Figure 11 is Figure 8 is a schematic diagram of the pusher state of the pusher in the application;

[0029] Figure 12 is a schematic diagram of the three-dimensional structure of an embodiment of the pusher dyeing machine provided in the application;

[0030] Figure 13 is a schematic diagram of an embodiment of the sample detection equipment provided in the application;

[0031] Figure 14 is a flowchart of an embodiment of the control method of the pusher dyeing machine provided in the application. DETAILED DESCRIPTION

[0032] The application will be described in further detail below with reference to the drawings and embodiments. It is particularly pointed out that the following embodiments are only for illustration of the application and do not limit the scope of the application. Similarly, the following embodiments are only part of the embodiments of the application, and all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the application.

[0033] The terms "first", "second", "third" in the present application are only for description purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined with "first", "second", "third" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise specifically limited. All directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present application are only used to explain the relative positional relationship, movement condition, etc. between components in a certain posture (as shown in the drawings), and if the certain posture changes, the directional indications also change accordingly. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device including a series of steps or units is not limited to the listed steps or units, but can optionally include steps or units not listed, or can optionally include other steps or units inherent to the process, method, product or device.

[0034] In this document, reference to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily mutually exclusive or alternative embodiments. It is explicitly and implicitly understood that the embodiments described herein can be combined with other embodiments.

[0035] Please refer to Figure 1 , Figure 2 and Figure 3 , Figure 1 is a schematic diagram of the three-dimensional assembly structure of the push piece device 10 according to an embodiment of the application, Figure 2 is a schematic diagram of the three-dimensional exploded structure of the push piece device 10 in Figure 1 , Figure 3 is a control diagram of the push piece device 10 in Figure 1 , The push piece device 10 in the embodiment includes a sample adding unit 11, an image unit 12, a controller 13 and a push piece unit 14.

[0036] Please refer toFigure 4 and Figure 5 , Figure 4 is Figure 2 a schematic diagram of a perspective structure of the sample adding unit 11, Figure 5 is Figure 4 a schematic diagram of a principle structure of the sample adding needle 112, wherein the sample adding unit 11 is used to add the sample onto the slide 110.

[0037] Specifically, the sample adding unit 11 comprises a sample adding power element 111 and a sample adding needle 112, the sample adding power element 111 is in communication with the sample adding needle 112, so as to collect the sample through the sample adding needle 112 and add the collected sample onto the slide 110, in the embodiment, the sample adding power element 111 is in communication with the sample adding needle 112 through a sample adding pipe 111a.

[0038] In order to improve the accuracy of the sample amount added onto the slide, the sample adding power element 111 can be selected as a high-precision sample adding pump, such as a plunger pump, a peristaltic pump, etc.

[0039] Further, the sample adding unit 11 further comprises a sample adding driving mechanism 113, the sample adding needle 112 is installed on the sample adding driving mechanism 113, so that the sample adding driving mechanism 113 drives the sample adding needle 112 to move.

[0040] In the embodiment, the sample adding driving mechanism 113 comprises a first sample adding driving assembly 113a and a second sample adding driving assembly 113b, the second sample adding driving assembly 113b is connected with the first sample adding driving assembly 113a and the sample adding needle 112 respectively, so that the first sample adding driving assembly 113a drives the sample adding needle 112 to reciprocate in a first sample adding direction A, and the second sample adding driving assembly 113b drives the sample adding needle 112 to reciprocate in a second sample adding direction B, in the embodiment, the first sample adding direction A is a horizontal direction, and the second sample adding direction B is a vertical direction.

[0041] Please refer to Figure 6 , Figure 7 , Figure 8 , Figure 9 , Figure 10 and Figure 11 , Figure 6 is Figure 1 a schematic diagram of a position of an embodiment of the push piece device 10, Figure 7 is Figure 1 a schematic diagram of a position of another embodiment of the push piece device 10, Figure 8 is Figure 2 a schematic diagram of a perspective structure of the push piece unit 14, Figure 9 is Figure 2 a schematic diagram of a perspective structure of the conveying unit 16, Figure 10 is Figure 2A perspective view of the middle supporting unit 17, Figure 11 is Figure 8 A schematic diagram of the push piece state of the middle push piece 120, the image acquisition unit 12 is used to acquire a sample image of the sample on the slide 110, the controller 13 is in communication connection with the image acquisition unit 12, and the controller 13 is used to determine the contact angle of the sample and the slide 110 according to the sample image, and determine the push piece parameter of the sample according to the contact angle.

[0042] Specifically, the controller 13 first controls the sample adding power element 111 to collect the sample and add the sample to the slide 110, and then after the image acquisition unit acquires the sample image of the sample, the controller 13 receives the sample image, thereby identifying the sample image. In actual application, in order to prevent the sample from splashing during the sample adding process, the controller 13 first controls the first sample adding driving assembly 113a to drive the sample adding needle 112 to move above the slide 110, then controls the second sample adding driving mechanism 113b to drive the sample adding needle 112 to descend to a position in contact with the slide 110, then controls the second sample adding driving mechanism 113b to drive the sample adding needle 112 to rise to a preset height, and finally controls the sample adding power element 111 to add the sample in the sample adding needle 112 to the slide 110. The preset height can be set according to actual conditions, such as 0-20mm.

[0043] It can be understood that when the sample is added to the slide 110, and after a period of time, the sample will spread on the slide 110. In this embodiment, the image acquisition unit 12 can acquire an image before the sample spreads, or an image after the sample spreads.

[0044] In this embodiment, the image acquisition unit 12 is arranged on the side of the slide 110, so that the image acquisition unit 12 acquires the sample image of the sample on the side of the slide 110, so that the controller 13 can better determine the contact angle of the sample and the slide 110.

[0045] Further, in this embodiment, the side of the slide 110 is also provided with a background plate 12a, and the background plate 12a is arranged opposite to the image acquisition unit 12. The background plate 12a is used to provide an acquisition background for the image acquisition unit 12 when the image acquisition unit 12 acquires the sample image, which can reduce the interference information of the image and facilitate subsequent detection.

[0046] Optionally, the image acquisition unit 12 can be selected from a camera, a CCD element or a CMOS element, etc., which is not limited.

[0047] The push piece unit 14 is used to spread the sample on the slide 110 and is in communication connection with the controller 13, and the controller 13 is further used to control the push piece unit 14 to push the slide 110 according to the push piece parameter.

[0048] Specifically, the controller 13 is used to determine the viscosity of the sample based on the contact angle and to determine the dispensing parameters based on the viscosity of the sample. The contact angle reflects the viscosity of the sample, thereby enabling the dispensing unit 14 to perform the dispensing operation according to the dispensing parameters corresponding to the viscosity of the sample, ensuring the high quality standard of the smear formed by coating the sample on the glass slide 110.

[0049] The controller 13 can store the correlation between sample volume N, contact angle J, viscosity M, and slide pushing parameter F in advance, as shown in the table below. When the controller 13 controls the sample addition unit 11 to add a certain amount of sample N to the glass slide 110, the controller 13 determines the contact angle J (J1, J2, J3) based on the sample image acquired by the image acquisition unit 12, then determines the sample viscosity M (M1, M2, M3) based on the correlation in the table below, and finally determines the slide pushing parameter F (F1, F2, F3). In practical applications, the correlation between contact angle J, viscosity M, and slide pushing parameter F can be obtained from experimental tests or from manual experience.

[0050]

[0051] The slide pushing unit 14 includes a first slide pushing driving mechanism 14a, a second slide pushing driving mechanism 14b, and a slide pushing assembly 14c. The slide pushing assembly 14c is used to mount the slide 120. The second slide pushing driving mechanism 14b is connected to the first slide pushing driving mechanism 14a and the slide pushing assembly 14c respectively, so that the first slide pushing driving mechanism 14a drives the slide 120 away from or close to the glass slide 110 in the first slide pushing direction C, and the second slide pushing driving mechanism 14b drives the slide 140 in the second slide pushing direction D to coat the sample onto the glass slide 110, thereby completing the slide pushing operation and forming a smear. In the embodiment, the first slide pushing direction C is a vertical direction, and the second slide pushing direction D is a horizontal direction.

[0052] The aforementioned pusher parameters can be as follows: Figure 11 The pushing angle α shown can also be the pushing speed or pushing time of the pusher 120 moving in the second pushing direction D, or the distance L between the pusher 120 and the glass slide 110 in the first pushing direction C. Of course, it can also be other pushing parameters. Therefore, in practical applications, the pushing parameters can be set according to actual needs, and there are no limitations on this.

[0053] Furthermore, in this embodiment, the controller 13 is also used to determine whether the sample quantity on the slide 110 meets the slide pushing standard. When the sample quantity is higher than the slide pushing standard, the controller 13 controls the sample addition unit 11 to extract part of the sample on the slide 110. When the sample quantity is lower than the slide pushing standard, the controller 13 controls the sample addition unit 11 to add additional sample on the slide 110.

[0054] For example, when the sample amount on the slide 110 is higher than the standard, the sample amount is too much, which causes the sample to be coated on the printing area of the slide 110 when the pushing unit 14 performs the pushing operation. Therefore, in this case, the controller 13 controls the sample adding unit 11 to extract part of the sample on the slide 110, so that the sample amount on the slide 110 meets the standard.

[0055] For another example, when the sample amount on the slide 110 is lower than the standard, the sample amount is too little, which causes the coating range of the sample on the slide 110 to be too small after the pushing unit 14 completes the pushing operation. Therefore, in this case, the controller 13 controls the sample adding unit 11 to add sample on the slide 110, so that the sample amount on the slide 110 meets the standard.

[0056] In this embodiment, the slide pushing device 10 further comprises a weight detection unit 15, which is in communication connection with the controller 13 and is used to detect the weight of the slide 110. The controller 13 is used to determine whether the sample amount on the slide 110 meets the standard according to the weight of the slide 110. For example, when the weight of the slide 110 is lower than a preset weight, it means that the sample amount is lower than the pushing standard, and vice versa.

[0057] Further, the slide pushing device 10 in this embodiment further comprises a conveying unit 16, which is used to convey the slide 110. A pushing position P1 is arranged along the movement track of the conveying unit 16. The pushing unit 14 is used to coat the sample on the slide 110 at the pushing position P1.

[0058] Specifically, the conveying unit 16 comprises a conveying driving mechanism 161 and a conveying bearing mechanism 162. The conveying bearing mechanism 162 is used to bear the slide 110. The conveying driving mechanism 161 is connected with the conveying bearing mechanism 162. The controller 13 is used to control the conveying driving mechanism 161 to drive the conveying bearing mechanism 162 to move. The pushing position P1 is arranged along the movement track of the conveying bearing mechanism 162.

[0059] Further, the slide pushing device 10 in this embodiment further comprises a bearing unit 17, which comprises a detection bearing mechanism 171. The sample adding unit is used to add sample on the first slide, so that the image acquisition unit acquires the sample image of the sample on the first slide. The controller 13 is used to determine the contact angle of the sample and the first slide according to the sample image, and determine the pushing parameter of the pushing unit according to the contact angle.

[0060] Specifically, the conveying bearing mechanism 162 is configured to bear the first slide, and the conveying unit 16 further comprises a first pushing driving mechanism 163 and a first pushing member 164, the first pushing driving mechanism 163 is connected with the first pushing member 164, so as to drive the first pushing member 164 to push the first slide from the conveying bearing mechanism 162 to the detection bearing mechanism 171, so that the sample adding unit 11 adds the sample on the first slide.

[0061] In an embodiment, the controller 13 is configured to control the pushing unit 14 to apply the sample on the first slide at the pushing position P1 according to the pushing parameter, so that in this embodiment, the sample adding operation of the sample adding unit 11, the image acquisition operation of the image acquisition unit 12 and the pushing operation of the pushing unit 14 are all completed on the first slide, in this way, the sample on the first slide can be used for both detection and pushing operation, so as to avoid waste of the sample.

[0062] Specifically, the slide pushing-out position P2 is further arranged along the movement track of the conveying bearing mechanism 162, and the bearing unit 17 further comprises a second pushing driving mechanism 172 and a second pushing member 173, the second pushing driving mechanism 172 is connected with the second pushing member 173, the controller 13 first controls the conveying driving mechanism 161 to drive the conveying bearing mechanism 162 to move the first slide to the slide pushing-out position P2, and controls the first pushing driving mechanism 163 to drive the first pushing member 164 to push the first slide from the conveying bearing mechanism 162 to the detection bearing mechanism 171, after the image acquisition unit 12 acquires the sample image of the sample on the first slide, the controller 13 controls the second pushing driving mechanism 172 to drive the second pushing member 173 to push the first slide after detection from the detection bearing mechanism 171 to the conveying bearing mechanism 173, then the controller 13 controls the conveying driving mechanism to drive the conveying bearing mechanism 162 to move the first slide to the pushing position P1, finally the controller 13 controls the pushing unit 14 to apply the sample on the slide 110.

[0063] In another embodiment, the sample adding unit 11 is further configured to add the same sample on the second slide, and the controller 13 is configured to control the pushing unit 14 to apply the sample on the second slide at the pushing position P1 according to the pushing parameter, so that the image acquisition operation of the image acquisition unit 12 and the pushing operation of the pushing unit 14 are not performed on the same slide, in this way, during the image acquisition of the image acquisition unit 12, the conveying driving mechanism 161 can drive the conveying bearing mechanism 162 to move the second slide to the pushing position P1, so as to save the time of the whole process and improve the efficiency.

[0064] It is understandable that the first and second slides mentioned above can be the same type of slide or different types of slides. When they are the same type of slide, the first and second slides are named only for the purpose of differentiation and do not limit the slides.

[0065] like Figure 7 As shown, in another embodiment, the image acquisition unit 12 is used to acquire sample images of the sample on the glass slide 110 on the transport unit 16, and the controller 13 is used to control the transport unit 16 to move the glass slide 110 to the slide push position P1 after the image acquisition unit 12 has completed the acquisition.

[0066] Specifically, a sample application position P3 is provided along the movement trajectory of the conveying and carrying mechanism 162. After the conveying and carrying mechanism 162 receives the glass slide 110, the controller 13 first controls the conveying drive mechanism 161 to drive the conveying and carrying mechanism 162 to move the glass slide 110 to the sample application position P3, and controls the sample application unit 11 to add the sample to the glass slide 110. After the image acquisition unit 12 finishes acquiring the image, the controller 13 controls the conveying drive mechanism 161 to drive the conveying and carrying mechanism 162 to move the glass slide 110 to the slide pushing position P1. In this way, the carrying unit 17 in the above embodiment is not required. The receiving of the glass slide 110, the acquisition of the image and the slide pushing operation can be completed directly through the conveying unit 16, which simplifies the structure of the slide pushing device 10 and the entire process, reduces costs and saves time.

[0067] Further reading Figure 2 and Figure 3 The slide pushing device 10 in this embodiment also includes a slide storage unit 18, which is used to store slides 110. A slide receiving position P4 is also provided along the movement trajectory of the conveying and carrying mechanism 162. The conveying and carrying mechanism 162 is used to receive the slides 110 stored in the slide storage unit 18 at the slide receiving position P4.

[0068] For example, in one of the above optional embodiments, the conveying drive mechanism 161 drives the conveying carrier mechanism 162 to move to the slide receiving position P4, so that the conveying carrier mechanism 162 receives the first slide stored in the slide storage unit 18 at the slide receiving position P4, and then moves the first slide 110 to the slide pushing position P2, thereby completing the subsequent sample addition, image acquisition and slide pushing operations.

[0069] For example, in another optional embodiment of the above, the conveying driving mechanism 161 drives the conveying carrier mechanism 162 to move to the slide receiving position P4, so that the conveying carrier mechanism 162 receives the first slide stored by the slide storage unit 18 at the slide receiving position P4, and then moves the first slide to the slide pushing-out position P2, and after the detection carrier mechanism 171 receives the first slide, the conveying driving mechanism 161 drives the conveying carrier mechanism 162 to move to the slide receiving position P4 again, so that the conveying carrier mechanism 162 receives the second slide stored by the slide storage unit 18, and then moves the second slide to the slide pushing position P1, and when the controller 13 completes the detection of the part of the sample on the first slide, the slide pushing unit 14 performs the slide pushing operation on another part of the sample on the second slide.

[0070] Further referring to Figure 2 and Figure 3 , the slide pushing device 10 in the embodiment further includes a printing unit 19, and a printing position P5 is further arranged along the movement track of the conveying carrier mechanism 162, and the printing unit 19 is used for performing slide printing at the printing position P5, such as performing slide printing on the first slide described above.

[0071] Please refer to Figure 12 , Figure 12 is a schematic perspective view of an embodiment of the slide pushing and dyeing machine 20 provided in the application, and the slide pushing and dyeing machine 20 in the embodiment includes the dyeing unit 21 and the slide pushing device 10 in the above embodiment.

[0072] Among them, the controller 13 is further in communication connection with the dyeing unit 21, and the controller 13 is further used for controlling the dyeing unit 21 to perform dyeing on the slide, and it can be understood that the dyeing unit 21 is used for dyeing the smear formed after the slide pushing operation of the slide pushing unit 14.

[0073] Please refer to Figure 13 , Figure 13 is a schematic block diagram of an embodiment of the sample detection equipment 30 provided in the application, and the sample detection equipment 30 in the embodiment includes the slide pushing and dyeing machine 20 in the above embodiment and the detection device 31.

[0074] Among them, the detection device 31 is used for detecting the sample and is in communication connection with the controller 13, the controller 13 is used for receiving the detection result of the detection device 31 and judging whether there is the slide pushing parameter of the sample in the database, and the controller 13 is further used for acquiring the slide pushing parameter of the sample when there is the slide pushing parameter of the sample in the database, and controlling the image acquisition unit 12 to acquire the sample image of the sample on the slide when there is no slide pushing parameter of the sample in the database.

[0075] It can be understood that the specific detection method of the detection device 31 can be determined according to the type of the sample. For example, if the sample is a gynecological sample, the detection device 31 uses the detection method of the gynecological sample. If the sample is a blood sample, the detection device 31 uses the detection method of the blood sample.

[0076] Please refer to Figure 14 , Figure 14 is a flowchart of an embodiment of a control method of the push film staining machine 20 provided by the present application. The control method in the embodiment includes:

[0077] S110: controlling the sample adding unit to add the ex vivo biological sample to the slide;

[0078] S120: controlling the image acquisition unit to acquire an image of the ex vivo biological sample on the slide to obtain a microscopic image;

[0079] S130: determining a contact angle of the ex vivo biological sample and the slide according to the microscopic image;

[0080] S140: determining a push film parameter of the push film unit according to the contact angle;

[0081] S150: controlling the push film unit to push the film on the slide according to the push film parameter, and controlling the staining unit to stain the slide.

[0082] It can be understood that the specific description of each step can be referred to the related description in the above-mentioned push film device 10 and the push film staining machine 20 embodiment, which will not be repeated here.

[0083] Unlike the prior art, the push film staining machine provided by the embodiment includes: a sample adding unit for adding a sample to a slide; an image acquisition unit for acquiring a sample image of the sample on the slide; a push film unit for coating the sample on the slide; a staining unit for staining the slide; a controller in communication connection with the image acquisition unit, the push film unit and the staining unit, the controller being configured to determine a contact angle of the sample and the slide according to the sample image, determine a push film parameter of the push film unit according to the contact angle, control the push film unit to push the film on the slide according to the push film parameter, and control the staining unit to stain the slide. The contact angle reflects the viscosity of the sample, so that the push film unit can perform the push film operation according to the push film parameter corresponding to the viscosity of the sample, and the high-quality standard of the smear formed by coating the sample on the slide is ensured.

[0084] ​The above merely describes some embodiments of the present application, and is not intended to limit the protection scope of the present application, and any equivalent device or equivalent process transformation using the content of the present application specification and drawings, or direct or indirect application in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A push-through dyeing machine, characterized in that The push piece dyeing machine comprises: a sample adding unit configured to add a sample onto a slide; an image acquisition unit configured to acquire a sample image of the sample on the slide; a push piece unit configured to apply the sample onto the slide; a dyeing unit configured to dye the slide; a controller communicatively connected to the image acquisition unit, the push piece unit and the dyeing unit, the controller configured to determine a contact angle of the sample with the slide according to the sample image, determine a push piece parameter of the push piece unit according to the contact angle, control the push piece unit to push the sample onto the slide according to the push piece parameter, and control the dyeing unit to dye the slide; the controller is further configured to determine whether a sample amount of the sample on the slide meets a push piece standard, control the sample adding unit to extract part of the sample on the slide when the sample amount is determined to be higher than the push piece standard, and control the sample adding unit to supplement the sample on the slide when the sample amount is determined to be lower than the push piece standard.

2. The jig dyeing machine according to claim 1, characterized by the controller is configured to determine a viscosity of the sample according to the contact angle, and determine the push piece parameter according to the viscosity of the sample.

3. The slide stainer of claim 1, wherein, The push piece dyeing machine further comprises a weight detection unit communicatively connected to the controller and configured to detect a weight of the slide, and the controller is configured to determine whether the sample amount of the sample on the slide meets the push piece standard according to the weight of the slide.

4. The slide stainer of claim 1, wherein, The image acquisition unit is arranged on a side of the slide.

5. The slide stainer of claim 4, wherein, The side of the slide is further provided with a background plate, and the background plate is arranged opposite to the image acquisition unit.

6. The slide stainer of claim 1, wherein, The push piece dyeing machine further comprises a conveying unit configured to convey the slide, a push piece position is arranged along a movement track of the slide conveyed by the conveying unit, and the push piece unit is configured to apply the sample onto the slide at the push piece position.

7. The jig dyeing machine according to claim 6, characterized by The sample adding unit is configured to add the sample onto a first slide, the push piece dyeing machine further comprises a detection bearing mechanism configured to bear the first slide, the image acquisition unit is configured to acquire a sample image of the sample on the first slide, and the controller is configured to determine a contact angle of the sample with the first slide according to the sample image, determine a push piece parameter of the push piece unit according to the contact angle, and control the push piece unit to apply the sample onto the first slide at the push piece position according to the push piece parameter. Or the sample adding unit is configured to add the same sample onto a first slide and a second slide, the push piece dyeing machine further comprises a detection bearing mechanism configured to bear the first slide, the image acquisition unit is configured to acquire a sample image of the sample on the first slide, and the controller is configured to determine a contact angle of the sample with the first slide according to the sample image, determine a push piece parameter of the push piece unit according to the contact angle, and control the push piece unit to apply the sample onto the second slide at the push piece position according to the push piece parameter.

8. The slide stainer of claim 6, wherein, The image acquisition unit is configured to acquire a sample image of the sample on the slide on the conveying unit, and the controller is configured to control the conveying unit to move the slide to the slide pushing position after the image acquisition unit finishes the acquisition.

9. A sample testing device, characterized by, The sample detection device comprises a detection apparatus and the slide pushing and staining machine according to any one of claims 1-8, the detection apparatus is configured to detect the sample and is communicatively connected with the controller, the controller is configured to receive the detection result of the detection apparatus and determine whether the slide pushing parameters of the sample exist in the database, and the controller is further configured to acquire the slide pushing parameters of the sample when the slide pushing parameters of the sample exist in the database, and control the image acquisition unit to acquire the sample image of the sample on the slide when the slide pushing parameters of the sample do not exist in the database.

10. A control method of a push piece dyeing machine, characterized by, The slide pushing and staining machine comprises a sample adding unit, an image acquisition unit, a slide pushing unit and a staining unit, and the control method comprises: controlling the sample adding unit to add the ex vivo biological sample to the slide; determining whether the sample amount of the ex vivo biological sample on the slide meets the slide pushing standard, controlling the sample adding unit to extract part of the ex vivo biological sample on the slide when it is determined that the sample amount is higher than the slide pushing standard, and controlling the sample adding unit to supplement the addition of the ex vivo biological sample on the slide when it is determined that the sample amount is lower than the slide pushing standard; controlling the image acquisition unit to acquire an image of the ex vivo biological sample on the slide to obtain a microscopic image; determining a contact angle of the ex vivo biological sample and the slide according to the microscopic image; determining slide pushing parameters of the slide pushing unit according to the contact angle; controlling the slide pushing unit to push the slide according to the slide pushing parameters, and controlling the staining unit to stain the slide.

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