Plant extract with skin barrier repairing effect and preparation method and application thereof

Abstract

The present invention discloses a plant extract with skin barrier repair efficacy, a preparation method and an application thereof. The plant extract is obtained by extracting Sargassum, Cyperus rotundus and Lycium barbarum with a supramolecular low eutectic solvent. The plant extract first replenishes moisture to the skin, instantly relieving the skin redness, itching, tingling and discomfort symptoms; then inhibits the expression of inflammatory factors, reduces the occurrence of inflammatory reactions, and can remove free radicals and some oxidation products, protect the skin from the attack of oxidative free radicals, and relieve skin discomfort; finally, rebuilds and strengthens the skin's natural protective layer, enhances the skin's own resistance and tolerance, and better copes with external stimuli and skin discomfort, thereby achieving a healthy state of the skin barrier from short-term relief to long-term maintenance, and preventing the recurrence of allergic reactions and damage to the skin barrier.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a plant extract with skin barrier repairing effect, a composition, and a preparation method and application thereof. Background Art

[0002] The structural basis of the skin barrier is mainly the stratum corneum, epidermal lipids, natural moisturizing factors, etc. A healthy skin barrier can resist harmful external physical and chemical factors such as ultraviolet rays, and has moisturizing, regulating and anti-inflammatory effects.

[0003] In recent years, people have been exposed to radiation from electronic devices such as computers and mobile phones for a long time, and have been staying up late, lacking sleep, and staying in air-conditioned rooms for a long time, which has led to a lack of moisture in the skin and an imbalance of oil and water in the skin. Over time, the skin barrier is damaged, and symptoms such as dryness, peeling, and erythema will appear. In addition, with the continuous development of the industry, more and more new products have appeared. Many people do not understand the nature of the product, over-cleaning, applying masks, exfoliating, and over-pursuing strong efficacy products (retinol), which ultimately causes some users to damage the skin barrier.

[0004] When the skin barrier is damaged, on the one hand, the skin's ability to resist external stimuli will decrease, moisturizing factors and anti-inflammatory factors will decrease, allergens will enter the skin more easily, and the skin will become dry and sensitive; on the other hand, when the skin barrier function is damaged, the skin's keratinocytes will produce pro-allergenic factors, which can cause repeated allergies, thereby causing great physical and psychological distress.

[0005] Therefore, it is particularly important to develop a raw material that can repair the skin barrier, soothe skin discomfort in the short term, repair barrier damage, maintain the healthy state of the skin barrier in the long term, and prevent the recurrence of allergic reactions. Summary of the invention

[0006] The purpose of the present invention is to provide a plant extract, composition and preparation method and application thereof with skin barrier repair effect. Based on the principle of "mild use, low irritation" and "monarch, minister, assistant and envoy", the main ingredients are from natural plants. Sargassum extract, Thistle extract and Black Wolfberry extract are selected for compounding. The obtained plant extract can not only replenish moisture instantly and relieve discomfort symptoms, but also reduce inflammatory response, strengthen the barrier, and repair the skin inside and outside.

[0007] The technical solution of the present invention is as follows:

[0008] The first aspect of the present invention provides a method for preparing a plant extract having skin barrier repairing effect, which comprises the steps of:

[0009] Provide sargassum, giant thistle, and black wolfberry;

[0010] Providing a deep eutectic solvent;

[0011] Mixing the Sargassum, giant thistle and black wolfberry to obtain a mixture;

[0012] The mixture is crushed and sieved to obtain powder;

[0013] The powder is mixed with the low eutectic solvent, and after ultrasonic treatment, the mixture is centrifuged to remove impurities and concentrated to obtain a plant extract with skin barrier repairing effect.

[0014] Optionally, the weight ratio of the Sargassum, the giant winged thistle, and the black wolfberry is 1-15:1-10:0.5-8.

[0015] Optionally, the weight ratio of the Sargassum, the giant winged thistle, and the black wolfberry is 8-13:6-10:3-6.

[0016] Optionally, the method for preparing the low eutectic solvent comprises the steps of: mixing a hydrogen bond acceptor and a hydrogen bond donor according to a preset molar ratio, and heating for a preset time at a preset temperature and a preset speed to obtain the low eutectic solvent;

[0017] The hydrogen bond acceptor is one or two of choline chloride, L-carnitine, and betaine, and the hydrogen bond donor is one of 1,3-butanediol, urea, 1,3-propylene glycol, ethylene glycol, glycerol, and glycerol;

[0018] And / or, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2-1:8.

[0019] Optionally, the powder is mixed with the low eutectic solvent in a mass volume ratio of 1 g:30-65 mL.

[0020] Optionally, the ultrasonic treatment time is 40-100 min, and the ultrasonic treatment temperature is 65-85°C.

[0021] The second aspect of the present invention provides a plant extract having skin barrier repairing efficacy, wherein the plant extract is prepared by the method for preparing the plant extract having skin barrier repairing efficacy described in the present invention.

[0022] The third aspect of the present invention provides a method for preparing a composition with skin barrier repair efficacy, which comprises the steps of: mixing the plant extract with skin barrier repair efficacy described in the present invention, pentanediol, and deionized water, stirring and sieving to obtain a composition with skin barrier repair efficacy.

[0023] Optionally, the preparation method specifically comprises the steps of:

[0024] After heating 60-85 parts of deionized water to 50-60° C., add 1-20 parts of plant extract and stir evenly until transparent to obtain solution 1;

[0025] After the solution 1 cools to room temperature, 5-15 parts of pentanediol are added, stirred evenly, and passed through 200-2000 mesh gauze to obtain a composition with skin barrier repairing effect.

[0026] The fourth aspect of the present invention provides a composition with skin barrier repair efficacy, wherein the composition is prepared by the preparation method of the composition with skin barrier repair efficacy described in the present invention.

[0027] The fifth aspect of the present invention provides a use of the composition with skin barrier repair efficacy of the present invention in the preparation of skin care products.

[0028] Beneficial effects: The present invention discloses a plant extract, composition, preparation method and application thereof with skin barrier repair efficacy. A low eutectic solvent is used to extract a certain proportion of Sargassum, Thistle and Black Wolfberry to obtain a plant extract. This plant extract first replenishes moisture to the skin, instantly relieving the skin redness, itching, tingling and discomfort symptoms; then inhibits the expression of inflammatory factors, reduces the occurrence of inflammatory reactions, and can remove free radicals and some oxidation products, protect the skin from the attack of oxidative free radicals, and relieve skin discomfort; finally, rebuilds and strengthens the skin's natural protective layer, enhances the skin's own resistance and tolerance, and better copes with external stimuli and skin discomfort, thereby achieving a transition from short-term relief to long-term maintenance of a healthy skin barrier, and preventing the recurrence of allergic reactions and damage to the skin barrier. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The extraction rate test results obtained by water, ethanol and different deep eutectic solvent extraction methods.

[0030] Figure 2 Results based on macrophage cytotoxicity assay.

[0031] Figure 3 Results of keratinocyte cytotoxicity assay.

[0032] Figure 4 Based on the results of mast cell cytotoxicity assay.

[0033] Figure 5 , Figure 6 Cell proliferation test results.

[0034] Figure 7 These are the test results for barrier repair related protein genes.

[0035] Figure 8 This is the result of hyaluronidase activity inhibition test.

[0036] Fig. 9 This is the result of the IL-6 inflammatory factor inhibition test.

[0037] Fig.10 This is the result of the TNF-α inflammatory factor inhibition test.

[0038] Fig.11 This is the Tivi sensitivity test result.

[0039] Fig.12 Test image results for Tivi700.

[0040] Fig.13 This is the result of the skin erythema E test.

[0041] Fig.14 This is the skin redness a* test result. DETAILED DESCRIPTION

[0042] The present invention provides a plant extract, a composition and a preparation method and application thereof with skin barrier repair efficacy. To make the purpose, technical scheme and effect of the present invention clearer and more specific, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0043] At present, the products used for repair on the market are mainly the following two categories: one is that the products use chemical ingredients or too many plant ingredients; the other is to solve the problem of skin barrier repair from a single perspective and in a short time. There are not many products that solve the problem of skin barrier repair from multiple dimensions and maintain stability in the long term. The inventors have found that the use of Sargassum extract, Big Wing Thistle extract, and Black Wolfberry extract can solve these drawbacks. Based on this, the purpose of this embodiment is to provide a plant extract that can be applied to skin barrier skin care products. The three natural plants (i.e., Sargassum, Big Wing Thistle, and Black Wolfberry) are combined and extracted in a certain proportion. The obtained plant extract can solve skin problems from the dimensions of nourishing and moisturizing, soothing and anti-inflammatory, strong barrier, and long-term stability, thereby more effectively exerting the repair effect.

[0044] Sargassum is a traditional Chinese medicinal seaweed. It has the effects of clearing away phlegm and heat, softening hard masses, and promoting diuresis. It is mainly used to treat goiter, scrofula, hernia, testicular swelling and pain, and edema caused by phlegm and fluid. In terms of skin care, it has the effects of moisturizing, soothing, stabilizing, and repairing the barrier.

[0045] Thistle, also known as big-fin thistle and Scottish thistle. In traditional medicine, thistle has a long history of medicinal use. In Xinjiang, Uyghur medicine uses it to treat skin diseases, while Chinese medicine believes that thistle "has a cool and cool effect, enters the liver meridian, cools blood and stops bleeding" and "the whole plant is used for medicinal purposes and has a hemostatic effect." Modern research shows that it is rich in bioactive ingredients such as flavonoids, sesquiterpenes and their lactones and sterol compounds, and has pharmacological activities such as anti-tumor, antibacterial, regulating cardiopulmonary function, and healing wounds. The document "Study on the repair effect of extracts from thistle plants on skin barrier function damage" mentioned that extracts from thistle plants have an effect on the expression of related proteins FLG and LOR in damaged human skin, and have the effect of repairing the epidermal barrier function.

[0046] Black wolfberry is sweet, neutral in nature, and rich in protein, wolfberry polysaccharides, amino acids, vitamins, minerals, trace elements and other nutrients. It is also rich in natural proanthocyanidins, which are the most effective natural water-soluble free radical scavenger, with an efficacy of 20 times that of VC and 50 times that of VE. It can resist the damage of ultraviolet rays to the skin, resist the oxidation of cells by the outside world, effectively remove the accumulation and precipitation of harmful free radicals in the skin, delay the aging of human cell tissues, protect collagen, and make the skin fresh and elastic.

[0047] The above plants are traditionally used as medicine, and have the effects of nourishing and moisturizing, strengthening the skin barrier, soothing and anti-inflammatory, etc. In this embodiment, these plants are compounded and extracted in a certain proportion to complement each other and synergize to achieve the effect of skin barrier repair.

[0048] Specifically, an embodiment of the present invention provides a method for preparing a plant extract having skin barrier repairing efficacy, which comprises the steps of:

[0049] Provide sargassum, giant thistle, and black wolfberry;

[0050] Providing a deep eutectic solvent;

[0051] Mixing the Sargassum, giant thistle and black wolfberry to obtain a mixture;

[0052] The mixture is crushed and sieved to obtain powder;

[0053] The powder is mixed with the low eutectic solvent, and after ultrasonic treatment, the mixture is centrifuged to remove impurities and concentrated to obtain a plant extract with skin barrier repairing effect.

[0054] Many skin problems are caused by dry skin, such as damaged skin barrier, redness and itchiness. In fact, as long as the moisture is stable, the skin can repair many problems by itself. Since Sargassum comes from the deep sea, it has a strong moisturizing ability, which can input moisture into skin cells, stimulate skin self-repair, and strengthen the skin's natural barrier. Combined with giant thistle and wolfberry, it has a good soothing and repairing effect, which is conducive to maintaining the healthy state of the skin barrier for a long time and preventing the recurrence of allergic reactions and barrier damage.

[0055] In one embodiment, the weight ratio of the sargassum, the giant winged thistle, and the black wolfberry is 1-15: 1-10: 0.5-8. Through efficacy tests of various ratios, it is found that the effect is better within this ratio range, and an effective balance is achieved between efficacy and cost.

[0056] Preferably, the weight ratio of the Sargassum, the giant winged thistle and the black wolfberry is 8-13:6-10:3-6.

[0057] Within this ratio range, the skin barrier repair efficacy of plant extracts can be further improved, and a more effective balance can be achieved between efficacy and cost.

[0058] More preferably, the weight ratio of the Sargassum, the giant thistle, and the black wolfberry is 10: 8: 5. Through efficacy tests of various ratios, it is found that this ratio has the best effect and achieves a maximum balance between efficacy and cost.

[0059] In one embodiment, the method for preparing the deep eutectic solvent comprises the steps of: mixing the hydrogen bond acceptor and the hydrogen bond donor according to a preset molar ratio, and heating them at a preset temperature and a preset speed for a preset time to obtain the deep eutectic solvent. The deep eutectic solvent prepared by the above method can effectively extract Sargassum, giant winged thistle, and black wolfberry.

[0060] In one embodiment, the hydrogen bond acceptor is one or two of choline chloride, L-carnitine, and betaine, and the hydrogen bond donor is one of 1,3-butanediol, urea, 1,3-propylene glycol, ethylene glycol, glycerol, and glycerol; the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2-1:8. Preferably, the hydrogen bond acceptor is betaine, and the hydrogen bond donor is urea. Betaine-urea ionic liquid can extract more flavonoids and polysaccharide components in plants and improve efficacy.

[0061] The above hydrogen bond acceptors and hydrogen bond donors are combined in a certain molar ratio to form a low eutectic solvent. These ingredients are also commonly used ingredients in the field of skin care products, so the plant extracts obtained after extraction can be directly used in skin care products.

[0062] In one embodiment, the preset temperature is 60-90° C., the preset time is 1-2 hours, and the preset rotation speed is 400-800 rpm (such as 400 rpm). Under this condition, a uniform and transparent low eutectic solvent can be obtained.

[0063] In one embodiment, in the step of crushing and sieving the mixture, the sieving may be through a 40-60 mesh sieve.

[0064] In one embodiment, the powder is mixed with the deep eutectic solvent in a mass volume ratio of 1 g: 30-65 mL. Within this ratio range, the deep eutectic solvent can effectively extract Sargassum, Thistle and Black Wolfberry.

[0065] In one embodiment, the ultrasonic treatment time is 40-100 min, and the ultrasonic treatment temperature is 65-85°C.

[0066] In one embodiment, the centrifugal impurity removal and concentration step specifically comprises: centrifuging at a speed of 2000-3500 rpm (such as 3000 rpm) to obtain a supernatant and a residue, further extracting the residue with the low eutectic solvent for 1-3 times (such as 2 times), collecting the supernatant, and concentrating. The centrifugation time is 20-30 minutes.

[0067] The embodiment of the present invention provides a plant extract having skin barrier repairing efficacy, wherein the plant extract is prepared by the preparation method of the plant extract having skin barrier repairing efficacy described in the embodiment of the present invention.

[0068] The plant extracts described in this embodiment contain Sargassum extract, Thistle extract, Black Wolfberry extract and low eutectic solvent. The plant extracts can solve skin problems from the dimensions of nourishing and moisturizing, soothing and anti-inflammatory, strong barrier, long-term stability, etc., so as to more effectively play the repair effect. The low eutectic solvent is also a common ingredient in the field of skin care products, so the plant extracts can be directly used in cosmetics.

[0069] The embodiment of the present invention provides a method for preparing a composition having skin barrier repairing effect, which comprises the steps of:

[0070] The plant extract with skin barrier repairing effect described in the embodiment of the present invention, pentylene glycol (as a preservative) and deionized water (as a diluent) are mixed, stirred and sieved to obtain a composition with skin barrier repairing effect.

[0071] In one embodiment, the preparation method specifically comprises the steps of:

[0072] After heating 60-85 parts of deionized water to 50-60° C., add 1-20 parts of plant extract and stir evenly until transparent to obtain solution 1;

[0073] After the solution 1 cools to room temperature, 5-15 parts of pentanediol are added, stirred evenly, and passed through 200-2000 mesh gauze to obtain a composition with skin barrier repairing effect.

[0074] An embodiment of the present invention provides a composition with skin barrier repair efficacy, wherein the composition is obtained by using the preparation method of the composition with skin barrier repair efficacy described in the embodiment of the present invention.

[0075] An embodiment of the present invention provides a use of the composition having skin barrier repairing efficacy as described above in the preparation of cosmetics.

[0076] Compared with the prior art, this embodiment has the following technical effects:

[0077] (1) The plant extract used in this embodiment is obtained by extraction using a low eutectic solvent. The obtained ingredients are safe and low-irritating. This extraction method is green and environmentally friendly. The plant extract obtained after extraction does not need to remove the solvent and can be directly used in the preparation of a composition with skin barrier repair effect. This solvent can also be used in cosmetics.

[0078] (2) Sargassum extract, thistle extract, and black wolfberry extract are compounded to obtain a composition with skin barrier repair effect. This composition first replenishes moisture to the skin, instantly relieving the symptoms of skin redness, itching, and tingling; then inhibits the expression of inflammatory factors and reduces the occurrence of inflammatory reactions. At the same time, it can remove free radicals and some oxidation products, protect the skin from the attack of oxidative free radicals, and relieve skin discomfort; finally, it rebuilds and strengthens the skin's natural protective layer, enhances the skin's own resistance and tolerance, and better copes with external stimuli and skin discomfort, thereby achieving a transition from short-term relief to long-term maintenance of a healthy skin barrier and preventing the recurrence of allergic reactions and barrier damage.

[0079] The present invention will be further described below by means of specific examples.

[0080] 70g of Sargassum, 80g of thistle and 50g of black wolfberry were cleaned and crushed, and passed through a 60-mesh sieve to obtain a powder. 10L of betaine-urea was mixed with the powder, ultrasonicated at 70°C for 80 minutes, centrifuged to remove impurities, and concentrated to obtain a plant extract with skin barrier repair effect.

[0081] The other steps are the same, except that the betaine-urea is replaced by water, ethanol, L-carnitine-1,3-propylene glycol, choline chloride-1,3-propylene glycol, betaine-1,3-propylene glycol, and betaine-1,3-butylene glycol, respectively.

[0082] The extraction rates obtained by water, ethanol and different kinds of deep eutectic solvents (the molar ratio of hydrogen bond acceptor to hydrogen bond donor is 1:4) were tested respectively. The test results are as follows: Figure 1 As shown. Figure 1 It can be seen that the polysaccharide extraction rate under betaine-urea is the highest.

[0083] Example 1

[0084] 65g of Sargassum, 80g of thistle and 50g of black wolfberry were cleaned and crushed, and passed through a 60-mesh sieve to obtain a powder. 9.75L of betaine-urea was mixed with the powder, ultrasonicated at 70°C for 80 minutes, centrifuged to remove impurities, and concentrated to obtain a plant extract with skin barrier repair effect.

[0085] 65 parts of deionized water are heated to 55° C., 15 parts of plant extract are added and stirred evenly until transparent to obtain a solution 1; after cooling to room temperature, 8 parts of pentanediol are added to the solution 1, stirred evenly, and passed through a 400-mesh gauze to obtain a composition 1 with skin barrier repair efficacy.

[0086] Example 2

[0087] 100g of Sargassum, 80g of thistle and 50g of black wolfberry were cleaned and crushed, and passed through a 60-mesh sieve to obtain a powder. 11.5L of betaine-urea was mixed with the powder, ultrasonicated at 70°C for 80 minutes, centrifuged to remove impurities, and concentrated to obtain a plant extract with skin barrier repair effect.

[0088] 65 parts of deionized water were heated to 55° C., 15 parts of plant extract were added and stirred evenly until transparent to obtain solution 1; after cooling to room temperature, 8 parts of pentanediol were added to the solution 1, stirred evenly, and passed through 400 mesh gauze to obtain a composition 2 with skin barrier repair effect.

[0089] Example 3

[0090] 100g of Sargassum, 105g of thistle and 50g of black wolfberry were cleaned and crushed, and passed through a 60-mesh sieve to obtain a powder. 12.75L of betaine-urea was mixed with the powder, ultrasonicated at 70°C for 80 minutes, centrifuged to remove impurities, and concentrated to obtain a plant extract with skin barrier repair effect.

[0091] Heat 65 parts of deionized water to 55° C., add 15 parts of plant extract and stir until transparent to obtain solution 1; after cooling to room temperature, add 8 parts of pentanediol to the solution 1, stir evenly, and pass through 400 mesh gauze to obtain composition 3 with skin barrier repair effect.

[0092] Test Example 1

[0093] Safety tests were performed on Examples 1-3:

[0094] 1. Based on the macrophage cytotoxicity test, the results are as follows Figure 2 As shown. Figure 2 It can be seen that the compositions obtained in Examples 1-3 are based on macrophages, and the compositions do not show obvious cytotoxicity within a concentration range of 1.25% (v / v).

[0095] 2. Based on the keratinocyte cytotoxicity test, the results are as follows Figure 3 As shown. Figure 3 It can be seen that the composition obtained in Examples 1-3 showed no obvious cytotoxicity based on keratinocytes within a concentration range of 0.1563% (v / v).

[0096] 3. Based on the mast cell cytotoxicity test, the results are as follows Figure 4 As shown. Figure 4 It can be seen that the compositions obtained in Examples 1-3 did not show obvious cytotoxicity based on mast cells within a concentration range of 0.0078% (v / v).

[0097] 4. Chicken embryo chorioallantoic membrane vascular test. The results are shown in Table 1 below.

[0098] Table 1. Experimental results of toxic effects of samples on chicken embryo chorioallantoic membrane

[0099]

[0100] As can be seen from the table above, the RC of compositions 1-3 on chicken chorioallantoic membrane 50 All were within the range of (95% CI: 23.9-48.54%) and were non-irritating.

[0101] Test Example 2

[0102] The following efficacy tests were performed for Examples 1-3:

[0103] 1. Cell proliferation test, the results are as follows Figure 5 and Figure 6 shown.

[0104] Compared with the BC group (blank control group), the PC group (positive control group) showed an increasing trend of cell viability at 24h, 48h, and 72h, and the positive control group had a proliferation effect, indicating that this test system is effective;

[0105] Compared with the BC group, compositions 1-3 showed an increasing trend of cell viability at 24h, 48h, and 72h of administration, indicating that the compositions have a certain repair ability, and the repair ability is: composition 3 ≥ composition 2 > composition 1.

[0106] 2. Barrier repair related protein gene test, the results are as follows Figure 7 shown.

[0107] Compared with the BC group, the expression of FLG and LOR genes in the PC group was significantly increased, indicating that the experimental system was effective;

[0108] Compared with the BC group, compositions 1-3 showed a significant improvement effect on the expression of FLG and LOR genes, indicating that the compositions also play a repairing role in the skin barrier, and the repair ability is: composition 3>composition 2>composition 1.

[0109] 3. Hyaluronidase activity inhibition test, the results are as follows Figure 8 As shown. Figure 8 It can be seen that the compositions obtained in Examples 1-3 have the effect of inhibiting the activity of hyaluronidase, so the compositions have anti-inflammatory effects, and the inhibitory ability is: Composition 2 ≥ Composition 3 Composition 1.

[0110] 4. IL-6 (pg / mL) inflammatory factor inhibition test, the results are as follows Fig. 9 shown.

[0111] Among them, BC is the blank control group, NC is the negative control group, and PC (dexamethasone) is the positive control group. When the 1-test method is used for statistical analysis, compared with the BC group, the significance is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; compared with the NC group, the significance is indicated by *, P-value < 0.05 is indicated by *, and P.value < 0.01 is indicated by **.

[0112] Test results:

[0113] Compared with the blank control group, the IL-6 secretion level in the negative control group was significantly increased (P<0.01), indicating that the stimulation conditions of this experiment were effective;

[0114] Compared with the negative control group, the IL-6 secretion level of the positive control group was significantly reduced (P<0.01), indicating that the positive control group in this experiment was effective;

[0115] Compared with the negative control group, the compositions obtained in Examples 1-3 can significantly reduce the IL-6 content, indicating that they have anti-inflammatory effects, and the anti-inflammatory ability is: composition 2>composition 3>composition 1.

[0116] 5. TNF-α inflammatory factor inhibition test, the results are as follows Fig.10 shown.

[0117] Among them, BC is the blank control group, NC is the negative control group, and PC (dexamethasone) is the positive control group. When the 1-test method is used for statistical analysis, compared with the BC group, the significance is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; compared with the NC group, the significance is indicated by *, P-value < 0.05 is indicated by *, and P.value < 0.01 is indicated by **.

[0118] Test results:

[0119] Compared with the blank control group, the secretion level of TNF-a in the negative control group was significantly increased (P<0.01), indicating that the stimulation conditions of this experiment were effective;

[0120] Compared with the negative control group, the secretion level of TNF-a in the positive control group was significantly reduced (P<0.01), indicating that the positive control group in this experiment was effective;

[0121] Compared with the negative control group, the compositions obtained in Examples 1-3 can significantly reduce the content of TNF-a, have a certain anti-inflammatory effect, and the anti-inflammatory ability is: Composition 2> Composition 3≥ Composition 1. .

[0122] From the test results of 1-5, it can be seen that Example 2 increases the amount of Sargassum on the basis of Example 1, so the expression of cell proliferation, FLG, LOR genes, and anti-inflammatory effect of Composition 2 is better than Composition 1; Example 3 increases the amount of Macrochiliaceae on the basis of Example 2, and the expression of FLG and LOR genes is improved, but the expression effect of cell proliferation is almost the same, and the anti-inflammatory effect is weakened. Therefore, from the perspective of repair ability and economy, the composition obtained in Example 2 has the best effect on repairing the skin barrier.

[0123] Test Example 3

[0124] Example 2 was selected to carry out lactic acid stinging test, and the results were as follows Figure 11-14 shown.

[0125] (1) Tivi sensitivity test:

[0126] In the blank group, the sensitivity (TiVi) fluctuated around the basal value at different test time points, and the difference was not statistically significant (P>0.05), indicating that the skin barrier function of the subjects remained stable and consistent during the test, so the influence of the subjects' own skin condition on the experimental results was negligible.

[0127] After the model was successfully established, the skin sensitivity of the negative control group slowly decreased over time, and the difference was statistically significant compared with after modeling (P<0.05); and the difference in skin sensitivity (TiVi) of the negative control group at 90 minutes after use was significantly lower than that of the blank group (P<0.05), indicating that the skin itself (negative control group) has a certain repair ability.

[0128] The composition obtained in Example 2 showed a significant decrease in the mean sensitivity (TiVi) at 40 min, 60 min and 90 min after use (p < 0.05), which was better than the negative control group, indicating that the composition can reduce skin sensitivity.

[0129] Tivi histogram combined with Tivi700 test image (such as Fig.12 As shown in the figure, it can be further illustrated that at 40min, 60min and 90min after the lactic acid stinging test, the composition obtained in Example 2 can promote skin soothing and reduce skin sensitivity.

[0130] (2) Skin erythema (E) test, the results are as follows Fig.13 shown.

[0131] In the blank group, skin erythema (E) fluctuated around the baseline value at different test time points, and the difference was not statistically significant (P>0.05), indicating that the skin barrier function of the subjects remained stable and consistent during the test, so the influence of the subjects' own skin condition on the experimental results was negligible.

[0132] After the model was successfully established in the negative control group, the skin sensitivity slowly decreased over time, and the difference was statistically significant compared with that after model establishment (P<0.05). Skin erythema (E) fluctuated around the baseline value, and the difference was not statistically significant (P>0.05), indicating that the redness of the skin remained stable and consistent during the test.

[0133] The composition obtained in Example 2 significantly decreased the skin erythema (E) value at 40 minutes, 60 minutes, and 90 minutes after use (p < 0.005), which was better than the negative control group, indicating that the composition has a better ability to improve the skin erythema state;

[0134] (3) Skin redness (a*) test, the results are as follows Fig.14 shown.

[0135] In the blank group, redness (a*) fluctuated around the basal value at different test time points, and the difference was not statistically significant (P>0.05), indicating that the skin barrier function of the subjects remained stable and consistent during the test, so the influence of the subjects' own skin condition on the experimental results was negligible.

[0136] After the model was successfully established in the negative control group, the skin sensitivity slowly decreased over time, and the difference was statistically significant compared with after the model was established (P<0.05); redness (a*) fluctuated around the baseline value, and the difference was not statistically significant (P>0.05), indicating that the redness of the skin remained stable and consistent during the test.

[0137] At 40 minutes, 60 minutes and 90 minutes after use, the redness (a*) value of the composition decreased significantly (p<0.05), which was better than that of the negative control group, indicating that the composition has better redness removal ability.

[0138] The lactic acid stinging test, combined with the test results of (1), (2) and (3), shows that the composition has a good effect of removing redness and soothing allergies.

[0139] Based on the above test results, it is shown that the composition of this embodiment can repair the skin barrier, relieve skin inflammation, and reduce redness caused by skin inflammatory reactions, and can be used in the field of skin barrier repair skin care products.

[0140] It should be understood that the application of the present invention is not limited to the above examples. For ordinary technicians in this field, improvements or changes can be made based on the above description. All these improvements and changes should fall within the scope of protection of the claims attached to the present invention.

Claims

1. A method for preparing a plant extract having skin barrier repairing effect, characterized in that: Includes steps: Provide sargassum, giant thistle, and black wolfberry; Providing a deep eutectic solvent; Mixing the Sargassum, giant thistle and black wolfberry to obtain a mixture; The mixture is crushed and sieved to obtain powder; The powder is mixed with the low eutectic solvent, and after ultrasonic treatment, impurities are removed by centrifugation and concentrated to obtain a plant extract with skin barrier repair effect; The method for preparing the low eutectic solvent comprises the steps of: mixing a hydrogen bond acceptor and a hydrogen bond donor according to a preset molar ratio, and heating them at a preset temperature and a preset speed for a preset time to obtain the low eutectic solvent; The hydrogen bond acceptor is betaine, and the hydrogen bond donor is urea; The molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2-1:8; The powder is mixed with the deep eutectic solvent in a mass volume ratio of 1 g: 30-65 mL; The weight ratio of the sargassum, giant winged thistle and black wolfberry is 8-13:6-10:3-6.

2. The method for preparing the plant extract having skin barrier repairing effect according to claim 1, characterized in that: The ultrasonic treatment time is 40-100 min, and the ultrasonic treatment temperature is 65-85°C.

3. A plant extract having skin barrier repairing effect, characterized in that: The product is prepared by the method for preparing the plant extract with skin barrier repairing effect as described in any one of claims 1 to 2.

4. A method for preparing a composition having skin barrier repairing effect, characterized in that: The method comprises the steps of: mixing the plant extract with skin barrier repairing effect as claimed in claim 3, pentanediol and deionized water, stirring and sieving to obtain a composition with skin barrier repairing effect.

5. The method for preparing the composition having skin barrier repairing effect according to claim 4, characterized in that: The preparation method specifically comprises the following steps, in parts by weight: After heating 60-85 parts of deionized water to 50-60° C., add 1-20 parts of plant extract and stir evenly until transparent to obtain solution 1; After the solution 1 cools to room temperature, 5-15 parts of pentanediol are added, stirred evenly, and passed through 200-2000 mesh gauze to obtain a composition with skin barrier repairing effect.

6. A composition having skin barrier repairing effect, characterized in that: The composition is prepared by the method for preparing the composition with skin barrier repairing effect as described in any one of claims 4 to 5.

7. Use of the composition with skin barrier repairing effect according to claim 6 in the preparation of cosmetics.

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